RLG00000027595

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017137
Physical Location & Seq
Reverse (-)
12994828 .. 12996875
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027595

Sequence Viewer

Length: 1128 bp
ATGGCAACCAAAGCAAGCCTATTTTTCATGCTTAGCTCCTTGTTTCTTCTTTGGAAGTTAAAAGTTAGCTCCTCGTTTCTTCTTCGGAATTTAAGCAATGCAGCATCATTGAAAGCTTGTAAGTTGGAAGCCATATATCAATTTGGGGACTCTTTCTCCGACACCGGAAATGCGATACGTGAAAACAAAGCTCTAGAGGCTACTAAGCTCCCTTATGGCTTAAATCTTCCCAGGGAACCAAAAGGTCGATTTTCCGACGGGATGTTGGTTATAGATTTTGAAGCACAAGCAGCTGGGCTTCCATTTCTTAAACCATATTTGGATCGGGAAGCGAAAGATCACCACAACGGAAAGAACTTTGCTATTAGTTTTGCTAGCGTTTTGCCAAAAGATGTTCTTAAAGAGAAATCAATATTCTTCCCAGAAGCAATGACAAACACTACTCTACATAAGCAAGTGGATTGGATGGCCACCTACTTCAATGAAACCCCCTATTTTATTAAAGACCGCGCAGAGAAGCTTAAGAGGTCACTCTTTTTCATCGGACCAATGGCACATGGTGATTATATGTATTCAATGGACTTGAGTCGTAAATCTTCAGAATGGGTAAAAAGCAATTTGGTTCCAGATGTTGTCCAAACCATCAAAGAAGCTGCCACAAGGGTCATTGCATTTGGTGCTGTGAAAGTGGTCATCCCAGGAATATACGCATTTGGTTGTTTTAAAGCTCCAGGTTATAATGTATCGCTATGCGACCAGGGCAAAAATAGTGTTGCAACCACTCACAACGATCTGCTTCAGAGAGCAATTGAGGAGTTAAAGAAACAACACCCTAATGTGACTATTGTATATGCAGATTATTACAATGCATTCATGTCCATCTTCCCCAAGGCACTTAGTCTAGGGTTTGAACCTGCGAACAAGCCATGCTGTTTTGGTACACACAAAAATCGTCCAGGACAAGATTTTAACCCGGATAACATGTGTGGAAAGCCTGGAGTACAAGCTTGTGCTAACCCTGCGAAACGAATCAACTGGGATGGTAGTCATATGACGCAACATGCATTCAAGTTAATTGCAGATTTGATAATTCCTGACATCGCAGCCAAGCTTAAATGCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00901 ko01110 map00901 map01110
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

376

Amino Acids

41.83

Weight (kDa)

9.13

Isoelectric Point (pI)

27.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 363 4e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03980
fragaria_vesca FvH4_4g05740
prunus_persica Prupe.4G256100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0363081
rosa_laevigata RLG00000027595
rosa_multiflora Rmu_sc0004472.1_g000006
rosa_roxburghii Rroxscaffold_4G00293480
rosa_rugosa Rorug01G0304500
rosa_samantha Rh1AG313500 Rh1BG276700 Rh1CG293400 Rh1DG307400
rosa_wichuraiana Rw1G027770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 922
AccII CGCG 1 cut(s) 510
AciI CCGC 1 cut(s) 508
AclWI GGATC 1 cut(s) 330
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 1 cut(s) 88
AcuI CTGAAG 2 cut(s) 582, 782
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 2 cut(s) 940, 1002
AflII CTTAAG 1 cut(s) 521
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 6 cut(s) 112, 281, 481, 576, 911, 1069
AjnI CCWGG 6 cut(s) 230, 697, 730, 756, 955, 994
AlwI GGATC 1 cut(s) 330
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 4 cut(s) 101, 290, 653, 1103
ApoI RAATTY 1 cut(s) 88
AspLEI GCGC 1 cut(s) 512
AspS9I GGNCC 1 cut(s) 545
AsuC2I CCSGG 1 cut(s) 974
AsuHPI GGTGA 2 cut(s) 332, 572
AsuNHI GCTAGC 1 cut(s) 374
AvaII GGWCC 1 cut(s) 545
BalI TGGCCA 1 cut(s) 470
BbvI GCAGC 4 cut(s) 113, 302, 640, 1115
BccI CCATC 4 cut(s) 460, 650, 887, 1034
BciT130I CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
BcnI CCSGG 1 cut(s) 974
BfaI CTAG 3 cut(s) 194, 375, 902
BfrI CTTAAG 1 cut(s) 521
BfuAI ACCTGC 1 cut(s) 922
BisI GCNGC 4 cut(s) 102, 291, 654, 1104
BlpI GCTNAGC 1 cut(s) 32
BlsI GCNGC 4 cut(s) 103, 292, 655, 1105
Bme1390I CCNGG 7 cut(s) 232, 699, 732, 758, 957, 974, 996
Bme18I GGWCC 1 cut(s) 545
BmgT120I GGNCC 1 cut(s) 545
BmiI GGNNCC 2 cut(s) 237, 624
BmrFI CCNGG 7 cut(s) 232, 699, 732, 758, 957, 974, 996
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 1 cut(s) 113
BmtI GCTAGC 1 cut(s) 378
BmuI ACTGGG 1 cut(s) 1045
BoxI GACNNNNGTC 1 cut(s) 585
BpmI CTGGAG 2 cut(s) 714, 1017
Bpu1102I GCTNAGC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 974
BsaAI YACGTR 1 cut(s) 179
BsaJI CCNNGG 5 cut(s) 230, 231, 697, 757, 888
BsaWI WCCGGW 1 cut(s) 164
BsaXI ACNNNNNCTCC 2 cut(s) 140, 170
Bse1I ACTGG 1 cut(s) 1040
Bse3DI GCAATG 3 cut(s) 103, 435, 666
BseBI CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
BseDI CCNNGG 5 cut(s) 230, 231, 697, 757, 888
BseGI GGATG 4 cut(s) 267, 471, 693, 1045
BseMI GCAATG 3 cut(s) 103, 435, 666
BseNI ACTGG 1 cut(s) 1040
BseRI GAGGAG 2 cut(s) 61, 827
BseXI GCAGC 4 cut(s) 113, 302, 640, 1115
BseYI CCCAGC 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 1 cut(s) 470
BsiSI CCGG 2 cut(s) 165, 974
BslFI GGGAC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 161
BsmI GAATGC 2 cut(s) 869, 1064
BsnI GGCC 1 cut(s) 470
Bsp143I GATC 3 cut(s) 322, 337, 790
Bsp1720I GCTNAGC 1 cut(s) 32
BspACI CCGC 1 cut(s) 508
BspANI GGCC 1 cut(s) 470
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 237, 624
BspMI ACCTGC 1 cut(s) 922
BspOI GCTAGC 1 cut(s) 378
BspPI GGATC 1 cut(s) 330
BspTI CTTAAG 1 cut(s) 521
BsrDI GCAATG 3 cut(s) 103, 435, 666
BsrI ACTGG 1 cut(s) 1040
BssECI CCNNGG 5 cut(s) 230, 231, 697, 757, 888
BssMI GATC 3 cut(s) 322, 337, 790
BssT1I CCWWGG 1 cut(s) 888
Bst2UI CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
BstAFI CTTAAG 1 cut(s) 521
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 2 cut(s) 16, 376
BstDEI CTNAG 3 cut(s) 32, 204, 896
BstF5I GGATG 4 cut(s) 267, 471, 693, 1045
BstFNI CGCG 1 cut(s) 510
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 3 cut(s) 325, 340, 793
BstMBI GATC 3 cut(s) 322, 337, 790
BstMWI GCNNNNNNNGC 6 cut(s) 11, 197, 290, 677, 759, 1019
BstNI CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
BstNSI RCATGY 2 cut(s) 985, 1064
BstPAI GACNNNNGTC 1 cut(s) 585
BstSCI CCNGG 7 cut(s) 230, 697, 730, 756, 955, 972, 994
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 4 cut(s) 113, 302, 640, 1115
BsuRI GGCC 1 cut(s) 470
BtgZI GCGATG 1 cut(s) 1084
BtsCI GGATG 4 cut(s) 267, 471, 693, 1045
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 1 cut(s) 1126
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 2 cut(s) 16, 376
CfoI GCGC 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 545
CseI GACGC 1 cut(s) 1063
Csp6I GTAC 2 cut(s) 939, 1001
CviAII CATG 6 cut(s) 28, 557, 874, 927, 982, 1061
CviQI GTAC 2 cut(s) 939, 1001
DdeI CTNAG 3 cut(s) 32, 204, 896
DpnI GATC 3 cut(s) 324, 339, 792
DpnII GATC 3 cut(s) 322, 337, 790
DraI TTTAAA 1 cut(s) 724
DraIII CACNNNGTG 1 cut(s) 560
EaeI YGGCCR 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 888
Eco47I GGWCC 1 cut(s) 545
Eco57I CTGAAG 2 cut(s) 582, 782
EcoRII CCWGG 6 cut(s) 230, 697, 730, 756, 955, 994
EcoT14I CCWWGG 1 cut(s) 888
EcoT22I ATGCAT 2 cut(s) 871, 1066
ErhI CCWWGG 1 cut(s) 888
FaeI CATG 6 cut(s) 31, 560, 877, 930, 985, 1064
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 1 cut(s) 161
FatI CATG 6 cut(s) 27, 556, 873, 926, 981, 1060
FauNDI CATATG 1 cut(s) 1050
Fnu4HI GCNGC 4 cut(s) 102, 291, 654, 1104
FokI GGATG 4 cut(s) 274, 478, 680, 1052
Fsp4HI GCNGC 4 cut(s) 102, 291, 654, 1104
FspBI CTAG 3 cut(s) 194, 375, 902
GlaI GCGC 1 cut(s) 511
GluI GCNGC 4 cut(s) 102, 291, 654, 1104
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 2 cut(s) 714, 1017
HaeIII GGCC 1 cut(s) 470
HapII CCGG 2 cut(s) 165, 974
HgaI GACGC 1 cut(s) 1063
HhaI GCGC 1 cut(s) 512
Hin1II CATG 6 cut(s) 31, 560, 877, 930, 985, 1064
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HindIII AAGCTT 4 cut(s) 114, 518, 1005, 1109
HinfI GANTC 3 cut(s) 149, 586, 1029
HpaII CCGG 2 cut(s) 165, 974
HphI GGTGA 2 cut(s) 332, 572
Hpy166II GTNNAC 1 cut(s) 941
Hpy188I TCNGA 6 cut(s) 87, 160, 256, 545, 601, 801
Hpy188III TCNNGA 4 cut(s) 194, 326, 626, 1094
Hpy8I GTNNAC 1 cut(s) 941
Hpy99I CGWCG 1 cut(s) 260
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 9 cut(s) 101, 671, 776, 854, 869, 1064, 1079, 1119, 1124
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 197, 290, 677, 759, 1019
HpyF3I CTNAG 3 cut(s) 32, 204, 896
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 6 cut(s) 31, 560, 877, 930, 985, 1064
HspAI GCGC 1 cut(s) 510
Kzo9I GATC 3 cut(s) 322, 337, 790
LmnI GCTCC 4 cut(s) 41, 74, 213, 733
Lsp1109I GCAGC 4 cut(s) 113, 302, 640, 1115
LweI GCATC 1 cut(s) 113
MaeI CTAG 3 cut(s) 194, 375, 902
MaeII ACGT 1 cut(s) 178
MaeIII GTNAC 2 cut(s) 528, 838
MalI GATC 3 cut(s) 324, 339, 792
MboI GATC 3 cut(s) 322, 337, 790
MboII GAAGA 7 cut(s) 38, 71, 74, 218, 409, 588, 874
MfeI CAATTG 1 cut(s) 807
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 6 cut(s) 88, 140, 616, 807, 1074, 1089
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 2 cut(s) 143, 595
MmeI TCCRAC 3 cut(s) 105, 183, 279
MnlI CCTC 4 cut(s) 82, 190, 519, 805
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 2 cut(s) 871, 1066
MscI TGGCCA 1 cut(s) 470
MslI CAYNNNNRTG 1 cut(s) 834
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 2 cut(s) 165, 974
MspR9I CCNGG 7 cut(s) 232, 699, 732, 758, 957, 974, 996
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 2 cut(s) 869, 1064
MvaI CCWGG 6 cut(s) 232, 699, 732, 758, 957, 996
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 6 cut(s) 11, 197, 290, 677, 759, 1019
NciI CCSGG 1 cut(s) 974
NdeI CATATG 1 cut(s) 1050
NdeII GATC 3 cut(s) 322, 337, 790
NheI GCTAGC 1 cut(s) 374
NlaIII CATG 6 cut(s) 31, 560, 877, 930, 985, 1064
NlaIV GGNNCC 2 cut(s) 237, 624
NmuCI GTSAC 2 cut(s) 528, 838
NsiI ATGCAT 2 cut(s) 871, 1066
NspI RCATGY 2 cut(s) 985, 1064
PasI CCCWGGG 1 cut(s) 231
PciI ACATGT 1 cut(s) 981
PctI GAATGC 2 cut(s) 869, 1064
PfeI GAWTC 1 cut(s) 1029
PfoI TCCNGGA 1 cut(s) 955
PkrI GCNGC 4 cut(s) 103, 292, 655, 1105
PleI GAGTC 2 cut(s) 143, 594
PpsI GAGTC 2 cut(s) 143, 594
Ppu21I YACGTR 1 cut(s) 179
PscI ACATGT 1 cut(s) 981
PshAI GACNNNNGTC 1 cut(s) 585
PsiI TTATAA 1 cut(s) 738
Psp6I CCWGG 6 cut(s) 230, 697, 730, 756, 955, 994
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 6 cut(s) 230, 697, 730, 756, 955, 994
PspN4I GGNNCC 2 cut(s) 237, 624
PspPI GGNCC 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 2 cut(s) 940, 1002
RsaNI GTAC 2 cut(s) 939, 1001
RseI CAYNNNNRTG 1 cut(s) 834
SatI GCNGC 4 cut(s) 102, 291, 654, 1104
Sau3AI GATC 3 cut(s) 322, 337, 790
Sau96I GGNCC 1 cut(s) 545
SchI GAGTC 2 cut(s) 143, 595
ScrFI CCNGG 7 cut(s) 232, 699, 732, 758, 957, 974, 996
SfaNI GCATC 1 cut(s) 113
SinI GGWCC 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 2 cut(s) 521, 583
SmoI CTYRAG 2 cut(s) 521, 583
Sse9I AATT 6 cut(s) 88, 140, 616, 807, 1074, 1089
SsiI CCGC 1 cut(s) 508
SspI AATATT 1 cut(s) 414
SspMI CTAG 3 cut(s) 194, 375, 902
StyD4I CCNGG 7 cut(s) 230, 697, 730, 756, 955, 972, 994
StyI CCWWGG 1 cut(s) 888
TaiI ACGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 247
TasI AATT 6 cut(s) 88, 140, 616, 807, 1074, 1089
TatI WGTACW 1 cut(s) 1000
TfiI GAWTC 1 cut(s) 1029
TscAI CASTG 1 cut(s) 1126
TseFI GTSAC 2 cut(s) 528, 838
TseI GCWGC 4 cut(s) 101, 290, 653, 1103
Tsp45I GTSAC 2 cut(s) 528, 838
TspDTI ATGAA 4 cut(s) 16, 498, 529, 862
TspGWI ACGGA 1 cut(s) 363
TspRI CASTG 1 cut(s) 1126
Vha464I CTTAAG 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 545
XapI RAATTY 1 cut(s) 88
XbaI TCTAGA 1 cut(s) 193
XceI RCATGY 2 cut(s) 985, 1064
XspI CTAG 3 cut(s) 194, 375, 902
Zsp2I ATGCAT 2 cut(s) 871, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.