FvH4_4g06230

Arginine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015592
Physical Location & Seq
Forward (+)
5578422 .. 5581903
3482 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06230.t1

Sequence Viewer

Length: 1689 bp
ATGCTCAGTGTTTCTGTGTTCTCAGCTTCCTCAGACCTTCAGACTTTGCATTTGGGTTTTAGGAATAACTGGACTTTCGGAAGACCACGCGCTTCTCTATCTCAGGAAAGTATAAATTTAGAGCCTAGAGAGAAGGAAGATGAAACAAGTTGGACAGTGACGGCCAGGTCTGCCACCAAGGATAAGAGTTTAGTTTCTAGCTCTCTGACGACTTCCAAGCAACAGAACTCTACTCCACTTGTCACTGCCTTGAAGGCTTCAGCTGAACAAAATGCCGCCACCTTTCACTTTCCTGGGCACAACAGAGGTCGAGCTGCACCAAATTCTATAACTGAACTTATTGGTTCCAAACCTTTTCTTCATGACTTACCTGAGCTTCCAGAACTTGACAACCTCTTTTCTCCGGAAGGGCCAATTTTAGATGCACAACAACAGGCAGCCAAACTCTTTGGCTCATTGGAGACATGGTTCCTTGTAGGAGGTACGACATGTGGAATTCAAGCAGCCATTATGGCTACTTGTTCCCCTGGAGATATTTTAGTTCTCCCTCGGAATTCCCATATATCAGCTGTATCGGCTTTAATATTGTCTGGTGCGGTACCCAAGTACATTATCCCTGATTATAACTCTGACTGGAACATTGCTGTTGGGGTCACTCCATCACAGGTGGAGACGGCTATCAAGGAAGTGGAGAAAGAAGGGCAAAAGGCAGCTGCTGTTTTCATCACTTCTCCAACATATCATGGTATATGCAGCAACTTGAGCGAGATAACCCAATTGTGCCATTCTCATGGGATCCCTGTGATTGTTGATGAGGCCCATGGAGCGCATTTGGGATTTCATCCCCATATGCCGAGTTCAGCCATCCAGCAAGGTGCTGATATAGCTGTCCAATCCACTCACAAGGTTCTTTGCTCGCTCACACAATCATCAATGTTGCACTTGTCTGGTACACTTGTAGATAGAGGAAAGATTTCTAGATGCCTTCAAACACTTCAAAGCAGTAGTCCTAGTTATCTTCTATTGGCATCATTAGATGCAGCTAGAGCTCAAATTAGTGAAGATCCTGAAACCATTTTTGATAAAGCATTGCAACTGGCTATTGAAGCTAGGAATATGTTGAGAAAGACTACTGGTATTTCAGTGCTTGATGTTACAAGCTTCCCAAAGTTTCCTGCAATTGATCCTTTGCGGCTTACTATAGGATTTCGACAGCTTGGCTTGTCTGGTTTTGAAGCTGATGAAATTTTGTACAAGGATCATGATATCATTTGTGAACTAGTTGAGACCCAATCCATTACTTTTGTAATTACCCTTGGAACTTGTAGAGAGCATGTTCAGAAGCTTGTATCAGGCATACAGCATATAGCAGCCGCTTCTCCATCATCTTGTGCTGCTAAAATGAAGGTGGACGGTAATAATCTTGCACCGTTTGCAGATATTAAAACTAGCTTGATTCCTAGAGATGCATTTTTTAGTGGTAAAAAGAGAGCGAGTATCGAAAACAGCTTGGGCGAGGTTTGTGGGGAGCTTATATGTCCATATCCACCTGGGATTCCAGTGTTGATTCCTGGAGAGATTATAACAAAGAAAGCTTTGGATTATCTGCTACATGTTAGAAGCAAGGGCGCTGTCATCACTGGTGCTTCTGATTCCCTACTTTCATCCATAGTTATCTGTAATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OKR_DC_1 OKR_DC_1_C

Protein Analysis

563

Amino Acids

60.32

Weight (kDa)

6.14

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OKR_DC_1 PF01276 78 - 385 4.4e-69 Orn/Lys/Arg decarboxylase, major domain
Aminotran_1_2 PF00155 141 - 357 4.8e-11 Aminotransferase class I and II
Beta_elim_lyase PF01212 141 - 302 9.1e-07 Beta-eliminating lyase
Aminotran_5 PF00266 155 - 304 6e-06 Aminotransferase class-V
DegT_DnrJ_EryC1 PF01041 162 - 276 5.5e-08 DegT/DnrJ/EryC1/StrS aminotransferase family
SepSecS PF05889 178 - 303 5.6e-06 O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS
OKR_DC_1_C PF03711 497 - 541 1.4e-07 Orn/Lys/Arg decarboxylase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015592)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06230 FvH4_4g06230 FvH4_4g06230
malus_domestica MD16G1239400.v1.1
prunus_persica Prupe.1G065500_v2.0.a1
pyrus_communis pycom16g20030
rosa_chinensis RchiOBHm_Chr4g0397951
rosa_laevigata RLG00000009394
rosa_multiflora Rmu_sc0000361.1_g000052 Rmu_sc0001074.1_g000007
rosa_roxburghii Rroxscaffold_5G00342850
rosa_rugosa Rorug04G0004800
rosa_samantha Rh4AG081100 Rh4DG074600
rosa_wichuraiana Rw0G020270 Rw4G006760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 624, 1583
AasI GACNNNNNNGTC 1 cut(s) 166
Acc65I GGTACC 1 cut(s) 598
AccB1I GGYRCC 1 cut(s) 598
AccII CGCG 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 403
AciI CCGC 4 cut(s) 276, 596, 1192, 1374
AclWI GGATC 5 cut(s) 790, 803, 1058, 1178, 1266
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 5 cut(s) 115, 322, 495, 553, 1245
AcuI CTGAAG 2 cut(s) 23, 243
AfaI GTAC 5 cut(s) 484, 600, 608, 952, 1253
AflIII ACRYGT 2 cut(s) 488, 1612
AgsI TTSAA 6 cut(s) 253, 500, 989, 998, 1106, 1235
AhlI ACTAGT 1 cut(s) 1279
AjnI CCWGG 5 cut(s) 164, 292, 526, 1549, 1570
AloI GAACNNNNNNTCC 2 cut(s) 452, 484
Alw21I GWGCWC 1 cut(s) 1051
Alw26I GTCTC 3 cut(s) 455, 665, 1280
AlwI GGATC 5 cut(s) 790, 803, 1058, 1178, 1266
AlwNI CAGNNNCTG 1 cut(s) 716
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 3 cut(s) 162, 410, 816
ApeKI GCWGC 9 cut(s) 314, 437, 503, 710, 713, 753, 1040, 1370, 1394
ApoI RAATTY 5 cut(s) 115, 322, 495, 553, 1245
ArsI GACNNNNNNTTYG 4 cut(s) 34, 66, 991, 1023
Asp700I GAANNNNTTC 1 cut(s) 973
Asp718I GGTACC 1 cut(s) 598
AspLEI GCGC 3 cut(s) 92, 829, 1631
AspS9I GGNCC 2 cut(s) 410, 817
BaeGI GKGCMC 1 cut(s) 300
BaeI ACNNNNGTAYC 4 cut(s) 590, 623, 942, 975
BamHI GGATCC 1 cut(s) 795
BanI GGYRCC 1 cut(s) 598
BanII GRGCYC 1 cut(s) 1051
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 1051
BbvI GCAGC 9 cut(s) 301, 449, 515, 700, 722, 765, 1052, 1381, 1382
BccI CCATC 3 cut(s) 667, 872, 1390
BceAI ACGGC 2 cut(s) 177, 690
BciT130I CCWGG 5 cut(s) 166, 294, 528, 1551, 1572
BcoDI GTCTC 3 cut(s) 455, 665, 1280
BcuI ACTAGT 1 cut(s) 1279
BfaI CTAG 9 cut(s) 126, 198, 978, 1011, 1044, 1110, 1280, 1449, 1461
BfmI CTRYAG 1 cut(s) 1200
BfoI RGCGCY 1 cut(s) 1632
BglI GCCNNNNNGGC 2 cut(s) 254, 512
Bme1390I CCNGG 5 cut(s) 166, 294, 528, 1551, 1572
BmgT120I GGNCC 2 cut(s) 410, 817
BmiI GGNNCC 4 cut(s) 346, 470, 600, 797
BmrFI CCNGG 5 cut(s) 166, 294, 528, 1551, 1572
BmsI GCATC 5 cut(s) 412, 971, 1027, 1037, 1456
BpiI GAAGAC 1 cut(s) 88
BpmI CTGGAG 2 cut(s) 549, 1593
Bpu10I CCTNAGC 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 781
BsaI GGTCTC 1 cut(s) 1280
BsaJI CCNNGG 7 cut(s) 177, 293, 526, 548, 820, 1315, 1550
BsaWI WCCGGW 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bse1I ACTGG 6 cut(s) 74, 638, 1101, 1138, 1559, 1645
Bse3DI GCAATG 2 cut(s) 639, 1088
BseAI TCCGGA 1 cut(s) 403
BseBI CCWGG 5 cut(s) 166, 294, 528, 1551, 1572
BseDI CCNNGG 7 cut(s) 177, 293, 526, 548, 820, 1315, 1550
BseGI GGATG 3 cut(s) 841, 864, 1664
BseMI GCAATG 2 cut(s) 639, 1088
BseMII CTCAG 5 cut(s) 19, 36, 45, 116, 363
BseNI ACTGG 6 cut(s) 74, 638, 1101, 1138, 1559, 1645
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 9 cut(s) 301, 449, 515, 700, 722, 765, 1052, 1381, 1382
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 164, 412, 818
BshNI GGYRCC 1 cut(s) 598
BsiHKAI GWGCWC 1 cut(s) 1051
BsiSI CCGG 1 cut(s) 404
BsmAI GTCTC 3 cut(s) 455, 665, 1280
BsmBI CGTCTC 1 cut(s) 665
BsnI GGCC 3 cut(s) 164, 412, 818
Bso31I GGTCTC 1 cut(s) 1280
Bsp1286I GDGCHC 2 cut(s) 300, 1051
Bsp13I TCCGGA 1 cut(s) 403
Bsp1407I TGTACA 1 cut(s) 1251
Bsp143I GATC 4 cut(s) 795, 1063, 1183, 1258
Bsp19I CCATGG 1 cut(s) 820
BspACI CCGC 4 cut(s) 276, 596, 1192, 1374
BspANI GGCC 3 cut(s) 164, 412, 818
BspCNI CTCAG 5 cut(s) 18, 35, 44, 115, 364
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 1 cut(s) 90
BspHI TCATGA 2 cut(s) 361, 1261
BspLI GGNNCC 4 cut(s) 346, 470, 600, 797
BspPI GGATC 5 cut(s) 790, 803, 1058, 1178, 1266
BspT107I GGYRCC 1 cut(s) 598
BspTNI GGTCTC 1 cut(s) 1280
BsrDI GCAATG 2 cut(s) 639, 1088
BsrGI TGTACA 1 cut(s) 1251
BsrI ACTGG 6 cut(s) 74, 638, 1101, 1138, 1559, 1645
BssECI CCNNGG 7 cut(s) 177, 293, 526, 548, 820, 1315, 1550
BssMI GATC 4 cut(s) 795, 1063, 1183, 1258
BssT1I CCWWGG 3 cut(s) 177, 820, 1315
Bst2UI CCWGG 5 cut(s) 166, 294, 528, 1551, 1572
Bst4CI ACNGT 3 cut(s) 157, 1415, 1431
BstAPI GCANNNNNTGC 1 cut(s) 1433
BstAUI TGTACA 1 cut(s) 1251
BstC8I GCNNGC 1 cut(s) 917
BstDEI CTNAG 5 cut(s) 5, 22, 31, 102, 372
BstDSI CCRYGG 1 cut(s) 820
BstF5I GGATG 3 cut(s) 841, 864, 1664
BstFNI CGCG 1 cut(s) 90
BstH2I RGCGCY 1 cut(s) 1632
BstHHI GCGC 3 cut(s) 92, 829, 1631
BstKTI GATC 4 cut(s) 798, 1066, 1186, 1261
BstMAI GTCTC 3 cut(s) 455, 665, 1280
BstMBI GATC 4 cut(s) 795, 1063, 1183, 1258
BstNI CCWGG 5 cut(s) 166, 294, 528, 1551, 1572
BstNSI RCATGY 3 cut(s) 492, 1337, 1616
BstSCI CCNGG 5 cut(s) 164, 292, 526, 1549, 1570
BstSFI CTRYAG 1 cut(s) 1200
BstSLI GKGCMC 1 cut(s) 300
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 9 cut(s) 301, 449, 515, 700, 722, 765, 1052, 1381, 1382
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 795, 1063
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 2 cut(s) 795, 1063
BsuRI GGCC 3 cut(s) 164, 412, 818
BtgI CCRYGG 1 cut(s) 820
BtsCI GGATG 3 cut(s) 841, 864, 1664
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 6 cut(s) 13, 162, 243, 1149, 1566, 1638
Cac8I GCNNGC 1 cut(s) 917
CaiI CAGNNNCTG 1 cut(s) 716
CciI TCATGA 2 cut(s) 361, 1261
CfoI GCGC 3 cut(s) 92, 829, 1631
Cfr13I GGNCC 2 cut(s) 410, 817
Csp6I GTAC 5 cut(s) 483, 599, 607, 951, 1252
CviAII CATG 9 cut(s) 362, 465, 489, 743, 791, 821, 1262, 1334, 1613
CviQI GTAC 5 cut(s) 483, 599, 607, 951, 1252
DdeI CTNAG 5 cut(s) 5, 22, 31, 102, 372
DpnI GATC 4 cut(s) 797, 1065, 1185, 1260
DpnII GATC 4 cut(s) 795, 1063, 1183, 1258
DrdI GACNNNNNNGTC 1 cut(s) 166
DseDI GACNNNNNNGTC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 162
Ecl136II GAGCTC 1 cut(s) 1049
Eco130I CCWWGG 3 cut(s) 177, 820, 1315
Eco24I GRGCYC 1 cut(s) 1051
Eco31I GGTCTC 1 cut(s) 1280
Eco32I GATATC 1 cut(s) 1267
Eco53kI GAGCTC 1 cut(s) 1049
Eco57I CTGAAG 2 cut(s) 23, 243
EcoICRI GAGCTC 1 cut(s) 1049
EcoRI GAATTC 2 cut(s) 495, 553
EcoRII CCWGG 5 cut(s) 164, 292, 526, 1549, 1570
EcoRV GATATC 1 cut(s) 1267
EcoT14I CCWWGG 3 cut(s) 177, 820, 1315
EcoT22I ATGCAT 1 cut(s) 1471
EcoT38I GRGCYC 1 cut(s) 1051
ErhI CCWWGG 3 cut(s) 177, 820, 1315
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 9 cut(s) 365, 468, 492, 746, 794, 824, 1265, 1337, 1616
FatI CATG 9 cut(s) 361, 464, 488, 742, 790, 820, 1261, 1333, 1612
FauNDI CATATG 1 cut(s) 849
FokI GGATG 3 cut(s) 828, 851, 1651
FriOI GRGCYC 1 cut(s) 1051
FspBI CTAG 9 cut(s) 126, 198, 978, 1011, 1044, 1110, 1280, 1449, 1461
GlaI GCGC 3 cut(s) 91, 828, 1630
GsuI CTGGAG 2 cut(s) 549, 1593
HaeII RGCGCY 1 cut(s) 1632
HaeIII GGCC 3 cut(s) 164, 412, 818
HapII CCGG 1 cut(s) 404
HhaI GCGC 3 cut(s) 92, 829, 1631
Hin1II CATG 9 cut(s) 365, 468, 492, 746, 794, 824, 1265, 1337, 1616
Hin6I GCGC 3 cut(s) 90, 827, 1629
HinP1I GCGC 3 cut(s) 90, 827, 1629
HindIII AAGCTT 3 cut(s) 1159, 1343, 1593
HinfI GANTC 4 cut(s) 1456, 1555, 1567, 1652
HpaII CCGG 1 cut(s) 404
Hpy166II GTNNAC 3 cut(s) 953, 1277, 1411
Hpy188I TCNGA 8 cut(s) 34, 42, 80, 207, 552, 631, 1341, 1651
Hpy188III TCNNGA 7 cut(s) 104, 362, 380, 404, 978, 1067, 1262
Hpy8I GTNNAC 3 cut(s) 953, 1277, 1411
HpyAV CCTTC 7 cut(s) 47, 127, 247, 401, 692, 995, 1399
HpyCH4III ACNGT 3 cut(s) 157, 1415, 1431
HpyF3I CTNAG 5 cut(s) 5, 22, 31, 102, 372
Hsp92II CATG 9 cut(s) 365, 468, 492, 746, 794, 824, 1265, 1337, 1616
HspAI GCGC 3 cut(s) 90, 827, 1629
Kpn2I TCCGGA 1 cut(s) 403
KpnI GGTACC 1 cut(s) 602
Kzo9I GATC 4 cut(s) 795, 1063, 1183, 1258
LmnI GCTCC 2 cut(s) 824, 1528
Lsp1109I GCAGC 9 cut(s) 301, 449, 515, 700, 722, 765, 1052, 1381, 1382
LweI GCATC 5 cut(s) 412, 971, 1027, 1037, 1456
MaeI CTAG 9 cut(s) 126, 198, 978, 1011, 1044, 1110, 1280, 1449, 1461
MaeIII GTNAC 4 cut(s) 157, 241, 652, 1153
MalI GATC 4 cut(s) 797, 1065, 1185, 1260
MboI GATC 4 cut(s) 795, 1063, 1183, 1258
MboII GAAGA 5 cut(s) 93, 149, 350, 1010, 1073
MfeI CAATTG 2 cut(s) 776, 1179
MflI RGATCY 2 cut(s) 795, 1063
MhlI GDGCHC 2 cut(s) 300, 1051
MmeI TCCRAC 2 cut(s) 131, 758
MnlI CCTC 8 cut(s) 40, 299, 404, 473, 558, 808, 959, 1510
Mph1103I ATGCAT 1 cut(s) 1471
MroI TCCGGA 1 cut(s) 403
MroXI GAANNNNTTC 1 cut(s) 973
MseI TTAA 2 cut(s) 581, 1443
MslI CAYNNNNRTG 1 cut(s) 789
MspA1I CMGCKG 3 cut(s) 263, 569, 713
MspI CCGG 1 cut(s) 404
MspR9I CCNGG 5 cut(s) 166, 294, 528, 1551, 1572
MunI CAATTG 2 cut(s) 776, 1179
MvaI CCWGG 5 cut(s) 166, 294, 528, 1551, 1572
MvnI CGCG 1 cut(s) 90
NcoI CCATGG 1 cut(s) 820
NdeI CATATG 1 cut(s) 849
NdeII GATC 4 cut(s) 795, 1063, 1183, 1258
NlaIII CATG 9 cut(s) 365, 468, 492, 746, 794, 824, 1265, 1337, 1616
NlaIV GGNNCC 4 cut(s) 346, 470, 600, 797
NmeAIII GCCGAG 1 cut(s) 879
NmuCI GTSAC 3 cut(s) 157, 241, 652
NsiI ATGCAT 1 cut(s) 1471
NspI RCATGY 3 cut(s) 492, 1337, 1616
PagI TCATGA 2 cut(s) 361, 1261
PciI ACATGT 2 cut(s) 488, 1612
PdmI GAANNNNTTC 1 cut(s) 973
PfeI GAWTC 4 cut(s) 1456, 1555, 1567, 1652
PfoI TCCNGGA 1 cut(s) 1570
PscI ACATGT 2 cut(s) 488, 1612
PsiI TTATAA 2 cut(s) 624, 1583
Psp124BI GAGCTC 1 cut(s) 1051
Psp6I CCWGG 5 cut(s) 164, 292, 526, 1549, 1570
PspGI CCWGG 5 cut(s) 164, 292, 526, 1549, 1570
PspN4I GGNNCC 4 cut(s) 346, 470, 600, 797
PspPI GGNCC 2 cut(s) 410, 817
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 2 cut(s) 795, 1063
PvuII CAGCTG 3 cut(s) 263, 569, 713
RsaI GTAC 5 cut(s) 484, 600, 608, 952, 1253
RsaNI GTAC 5 cut(s) 483, 599, 607, 951, 1252
RseI CAYNNNNRTG 1 cut(s) 789
SacI GAGCTC 1 cut(s) 1051
SaqAI TTAA 2 cut(s) 581, 1443
Sau3AI GATC 4 cut(s) 795, 1063, 1183, 1258
Sau96I GGNCC 2 cut(s) 410, 817
ScrFI CCNGG 5 cut(s) 166, 294, 528, 1551, 1572
SduI GDGCHC 2 cut(s) 300, 1051
SfaNI GCATC 5 cut(s) 412, 971, 1027, 1037, 1456
SfcI CTRYAG 1 cut(s) 1200
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 1 cut(s) 760
SmoI CTYRAG 1 cut(s) 760
SpeI ACTAGT 1 cut(s) 1279
SsiI CCGC 4 cut(s) 276, 596, 1192, 1374
SspI AATATT 1 cut(s) 585
SspMI CTAG 9 cut(s) 126, 198, 978, 1011, 1044, 1110, 1280, 1449, 1461
SstI GAGCTC 1 cut(s) 1051
StyD4I CCNGG 5 cut(s) 164, 292, 526, 1549, 1570
StyI CCWWGG 3 cut(s) 177, 820, 1315
TaaI ACNGT 3 cut(s) 157, 1415, 1431
TaqI TCGA 3 cut(s) 310, 1210, 1500
TatI WGTACW 2 cut(s) 606, 1251
TauI GCSGC 3 cut(s) 278, 1195, 1376
TfiI GAWTC 4 cut(s) 1456, 1555, 1567, 1652
Tru1I TTAA 2 cut(s) 581, 1443
Tru9I TTAA 2 cut(s) 581, 1443
TscAI CASTG 6 cut(s) 13, 162, 250, 1149, 1566, 1645
TseFI GTSAC 3 cut(s) 157, 241, 652
TseI GCWGC 9 cut(s) 314, 437, 503, 710, 713, 753, 1040, 1370, 1394
Tsp45I GTSAC 3 cut(s) 157, 241, 652
TspDTI ATGAA 7 cut(s) 156, 350, 712, 830, 1257, 1418, 1653
TspRI CASTG 6 cut(s) 13, 162, 250, 1149, 1566, 1645
XapI RAATTY 5 cut(s) 115, 322, 495, 553, 1245
XbaI TCTAGA 1 cut(s) 977
XceI RCATGY 3 cut(s) 492, 1337, 1616
XmnI GAANNNNTTC 1 cut(s) 973
XspI CTAG 9 cut(s) 126, 198, 978, 1011, 1044, 1110, 1280, 1449, 1461
Zsp2I ATGCAT 1 cut(s) 1471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.