MD16G1239400.v1.1

Arginine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015592
Physical Location & Seq
Forward (+)
25392882 .. 25396109
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1239400.v1.1.491

Sequence Viewer

Length: 1701 bp
ATGTTTTCAACTTCCTCAGCATCGTCCTTAAACTTTAGTAGTTTTCATTTGGGTCTTGGAAATAACTGGAATTTCAGGACTTGTGCAAGACCAACCGCTTGTCTTTCCCAGTCCCAGGAAAGTGGGAATCTAGAGCCAATCAAGAAGCAAGATAAAACTAGTGGGATAGGAAAAACCAGGTCTGCCACGAAGGATCAGGTCGTCATTTCCAACTCTCAGGTTTCTCAGCAGGAGAGCTCCCCTCCACTGATTACTGCATTGAAGGCTTCAGCTGAACAAAGTGCTGCTACATTTCACTTCCCTGGGCACAACAGAGGTCGAGCTGCACCATCTTCTTTAACTAAGCTTATTGGCTTAAAGCCATTTGTTCATGATTTGCCTGAGCTTCCTGATCTTGACGACCTCTTTTCCCCGAAAGGGCCCATTTTAGAAGCACAACAACAGGCGGCCAGTCTCTTTGGGTCAACCGAGACATGGTTCCTTGTAGGAGGAACCACATGTGGAATTCAGGCAGCAATTATGGCTACATGTTCCCCTGGAGAAATTTTAATTCTCCCTCGCAATTCGCATATATCAGCCATATCTGCTATGATATTGTCGGGTGCAGTACCTAAGTACATCATCCCGCATTATAATTTTCACTGGGACATTGCTGGTGGGGTCACTCCATCACAGGTAGAGGCTGCAATCAAGGAAGTGGAGAAAGAAGGCCGAAAAGCAGCTGCAGTTTTGGTCACTTCCCCCACTTATCATGGTATATGCAGCAACGTGAGGGAGATAACCCAGTTGTGCCATTCTCATGGAATTCCTGTGATTGTTGATGAGGCTCATGGCGCACATCTAGGATTTCATCCTGAGATGCCATATTCGGCCATGCAGCAAGGTGCTGACCTAGCCGTTCAATCCACTCACAAGGTTCTCTGCTCTCTCACACAATCATCAATGTTGCATATGTCTGGGAGTATTGTAGATAGAGAGAGAATTTCAAGATGTCTTCAAACGCTTCAAAGTAGTAGTCCTAGTTATCTGCTTCTGGCATCATTAGATGCTGCTAGAGCTCAAATTAGTGAAAATCCAGAAAATATTTTTGATAAAGCGTTACAGTTGGCCATTGAAGTGAAGAATACGATAAGAAAAATTTCAGGTATTTCAGTTCTCGACCATCAAAGCTTCCCTAATTTCCCTTCAACTGATCCCCTGCGGCTTACCATAGGATTCCAACAGCTTGGCTTGTCCGGTTATGAAGCCGATGAAATCTTGTACAAGGATCATGAAATCATTTGTGAACTTGTTGAAACCCAATCCATTACTTTTGCACTTAACCTTGGAACCTGTAGAGAGCATGTCCAGAGGTTATTATCGGGAATAAAGCACCTGGTGGCCGCTTCTGCATCAACTCAGGCTGCTAAAAGGAAAGTGGAAGGAGTTGGTGAGTCTGCAATCTTTGCAGAAATTGAAACAAGCTTAGTTCCTAGAGATGCCTTTTTTAGTGGTAAAAGAAGAGTGAGGGTTGAAAACAGCCTTGGGGAAATTTGTGGGGAGCTTATATGTTCATATCCGCCTGGGATTCCGGTAATAATCCCGGGGGAGACTATCACAAAGAAAGCTTTGGATTATCTGCTACATGTTAGAAGCAAAGGTGCTGTAATCAGTGGAGCTTCTGATCCCGAACTCGCTTCCATAGTTGTCTGCAGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OKR_DC_1 OKR_DC_1_C

Protein Analysis

567

Amino Acids

60.87

Weight (kDa)

6.47

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OKR_DC_1 PF01276 81 - 386 8.2e-71 Orn/Lys/Arg decarboxylase, major domain
Aminotran_1_2 PF00155 143 - 389 1.9e-10 Aminotransferase class I and II
Beta_elim_lyase PF01212 144 - 308 3.2e-09 Beta-eliminating lyase
Aminotran_5 PF00266 145 - 307 5e-06 Aminotransferase class-V
DegT_DnrJ_EryC1 PF01041 165 - 279 2.6e-06 DegT/DnrJ/EryC1/StrS aminotransferase family
SepSecS PF05889 179 - 307 4.1e-06 O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS
OKR_DC_1_C PF03711 499 - 545 4.7e-08 Orn/Lys/Arg decarboxylase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015592)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06230 FvH4_4g06230 FvH4_4g06230
malus_domestica MD16G1239400.v1.1
prunus_persica Prupe.1G065500_v2.0.a1
pyrus_communis pycom16g20030
rosa_chinensis RchiOBHm_Chr4g0397951
rosa_laevigata RLG00000009394
rosa_multiflora Rmu_sc0000361.1_g000052 Rmu_sc0001074.1_g000007
rosa_roxburghii Rroxscaffold_5G00342850
rosa_rugosa Rorug04G0004800
rosa_samantha Rh4AG081100 Rh4DG074600
rosa_wichuraiana Rw0G020270 Rw4G006760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AciI CCGC 6 cut(s) 96, 446, 626, 1201, 1383, 1559
AclWI GGATC 4 cut(s) 201, 1187, 1275, 1658
AcoI YGGCCR 4 cut(s) 447, 870, 1107, 1380
AcsI RAATTY 7 cut(s) 70, 504, 543, 804, 981, 1137, 1530
AcuI CTGAAG 1 cut(s) 252
AdeI CACNNNGTG 1 cut(s) 1378
AfaI GTAC 3 cut(s) 609, 617, 1262
AfiI CCNNNNNNNGG 3 cut(s) 115, 417, 474
AflIII ACRYGT 3 cut(s) 497, 527, 1624
AhlI ACTAGT 1 cut(s) 158
AjnI CCWGG 6 cut(s) 114, 176, 301, 535, 1374, 1561
AjuI GAANNNNNNNTTGG 2 cut(s) 1506, 1538
Alw21I GWGCWC 2 cut(s) 239, 1060
Alw26I GTCTC 3 cut(s) 458, 464, 1583
AlwI GGATC 4 cut(s) 201, 1187, 1275, 1658
Ama87I CYCGRG 1 cut(s) 1582
AoxI GGCC 6 cut(s) 419, 447, 709, 870, 1107, 1380
ApaI GGGCCC 1 cut(s) 423
ApoI RAATTY 7 cut(s) 70, 504, 543, 804, 981, 1137, 1530
ArsI GACNNNNNNTTYG 2 cut(s) 1000, 1032
Asp700I GAANNNNTTC 1 cut(s) 1138
AspLEI GCGC 1 cut(s) 836
AspS9I GGNCC 2 cut(s) 419, 420
AsuC2I CCSGG 2 cut(s) 1583, 1584
AsuHPI GGTGA 1 cut(s) 1442
AvaI CYCGRG 1 cut(s) 1582
BaeGI GKGCMC 2 cut(s) 309, 423
BalI TGGCCA 1 cut(s) 1109
BanII GRGCYC 3 cut(s) 239, 423, 1060
BbsI GAAGAC 1 cut(s) 986
Bbv12I GWGCWC 2 cut(s) 239, 1060
BbvCI CCTCAGC 1 cut(s) 16
BccI CCATC 3 cut(s) 337, 676, 1170
BceAI ACGGC 1 cut(s) 881
BciT130I CCWGG 6 cut(s) 116, 178, 303, 537, 1376, 1563
BcnI CCSGG 2 cut(s) 1583, 1584
BcoDI GTCTC 3 cut(s) 458, 464, 1583
BcuI ACTAGT 1 cut(s) 158
BfaI CTAG 7 cut(s) 131, 159, 842, 893, 1020, 1053, 1473
BfmI CTRYAG 3 cut(s) 723, 1333, 1690
Bme1390I CCNGG 8 cut(s) 116, 178, 303, 537, 1376, 1563, 1583, 1584
BmeT110I CYCGRG 1 cut(s) 1582
BmgT120I GGNCC 2 cut(s) 419, 420
BmiI GGNNCC 4 cut(s) 421, 479, 493, 1330
BmrFI CCNGG 8 cut(s) 116, 178, 303, 537, 1376, 1563, 1583, 1584
BmrI ACTGGG 3 cut(s) 103, 652, 778
BmsI GCATC 6 cut(s) 29, 849, 1036, 1046, 1400, 1468
BmuI ACTGGG 3 cut(s) 103, 652, 778
BpiI GAAGAC 1 cut(s) 986
BplI GAGNNNNNCTC 2 cut(s) 226, 258
BpmI CTGGAG 1 cut(s) 558
Bpu10I CCTNAGC 2 cut(s) 16, 381
BpuMI CCSGG 2 cut(s) 1583, 1584
BsaJI CCNNGG 9 cut(s) 114, 301, 302, 535, 1324, 1522, 1562, 1582, 1583
BsaWI WCCGGW 2 cut(s) 1235, 1570
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 417, 474
Bse1I ACTGG 5 cut(s) 71, 109, 450, 647, 784
Bse3DI GCAATG 1 cut(s) 648
BseBI CCWGG 6 cut(s) 116, 178, 303, 537, 1376, 1563
BseDI CCNNGG 9 cut(s) 114, 301, 302, 535, 1324, 1522, 1562, 1582, 1583
BseGI GGATG 2 cut(s) 621, 850
BseLI CCNNNNNNNGG 3 cut(s) 115, 417, 474
BseMI GCAATG 1 cut(s) 648
BseMII CTCAG 6 cut(s) 30, 230, 239, 372, 846, 1412
BseNI ACTGG 5 cut(s) 71, 109, 450, 647, 784
BseSI GKGCMC 2 cut(s) 309, 423
BsgI GTGCAG 2 cut(s) 309, 624
BshFI GGCC 6 cut(s) 421, 449, 711, 872, 1109, 1382
BsiHKAI GWGCWC 2 cut(s) 239, 1060
BsiHKCI CYCGRG 1 cut(s) 1582
BsiSI CCGG 3 cut(s) 1236, 1571, 1583
BslFI GGGAC 2 cut(s) 97, 659
BslI CCNNNNNNNGG 3 cut(s) 115, 417, 474
BsmAI GTCTC 3 cut(s) 458, 464, 1583
BsmFI GGGAC 2 cut(s) 97, 659
BsnI GGCC 6 cut(s) 421, 449, 711, 872, 1109, 1382
BsoBI CYCGRG 1 cut(s) 1582
Bsp120I GGGCCC 1 cut(s) 419
Bsp1286I GDGCHC 4 cut(s) 239, 309, 423, 1060
Bsp1407I TGTACA 1 cut(s) 1260
Bsp143I GATC 5 cut(s) 193, 391, 1192, 1267, 1663
BspACI CCGC 6 cut(s) 96, 446, 626, 1201, 1383, 1559
BspANI GGCC 6 cut(s) 421, 449, 711, 872, 1109, 1382
BspCNI CTCAG 6 cut(s) 29, 229, 238, 373, 847, 1411
BspHI TCATGA 2 cut(s) 370, 1270
BspLI GGNNCC 4 cut(s) 421, 479, 493, 1330
BspMAI CTGCAG 2 cut(s) 727, 1694
BspPI GGATC 4 cut(s) 201, 1187, 1275, 1658
BsrDI GCAATG 1 cut(s) 648
BsrGI TGTACA 1 cut(s) 1260
BsrI ACTGG 5 cut(s) 71, 109, 450, 647, 784
BssECI CCNNGG 9 cut(s) 114, 301, 302, 535, 1324, 1522, 1562, 1582, 1583
BssMI GATC 5 cut(s) 193, 391, 1192, 1267, 1663
BssT1I CCWWGG 2 cut(s) 1324, 1522
Bst2UI CCWGG 6 cut(s) 116, 178, 303, 537, 1376, 1563
Bst4CI ACNGT 1 cut(s) 1104
Bst6I CTCTTC 1 cut(s) 1495
BstAPI GCANNNNNTGC 1 cut(s) 1445
BstAUI TGTACA 1 cut(s) 1260
BstDEI CTNAG 9 cut(s) 16, 216, 225, 342, 381, 612, 855, 1398, 1465
BstF5I GGATG 2 cut(s) 621, 850
BstHHI GCGC 1 cut(s) 836
BstKTI GATC 5 cut(s) 196, 394, 1195, 1270, 1666
BstMAI GTCTC 3 cut(s) 458, 464, 1583
BstMBI GATC 5 cut(s) 193, 391, 1192, 1267, 1663
BstMWI GCNNNNNNNGC 8 cut(s) 263, 521, 584, 833, 893, 1055, 1388, 1445
BstNI CCWGG 6 cut(s) 116, 178, 303, 537, 1376, 1563
BstNSI RCATGY 4 cut(s) 501, 531, 1346, 1628
BstSCI CCNGG 8 cut(s) 114, 176, 301, 535, 1374, 1561, 1581, 1582
BstSFI CTRYAG 3 cut(s) 723, 1333, 1690
BstSLI GKGCMC 2 cut(s) 309, 423
BstV2I GAAGAC 1 cut(s) 986
BstXI CCANNNNNNTGG 3 cut(s) 122, 800, 1226
BsuRI GGCC 6 cut(s) 421, 449, 711, 872, 1109, 1382
BtsCI GGATG 2 cut(s) 621, 850
BtsIMutI CAGTG 3 cut(s) 245, 640, 1657
CciI TCATGA 2 cut(s) 370, 1270
CfoI GCGC 1 cut(s) 836
Cfr13I GGNCC 2 cut(s) 419, 420
Cfr9I CCCGGG 1 cut(s) 1582
CsiI ACCWGGT 2 cut(s) 176, 1374
Csp6I GTAC 3 cut(s) 608, 616, 1261
CviQI GTAC 3 cut(s) 608, 616, 1261
DdeI CTNAG 9 cut(s) 16, 216, 225, 342, 381, 612, 855, 1398, 1465
DpnI GATC 5 cut(s) 195, 393, 1194, 1269, 1665
DpnII GATC 5 cut(s) 193, 391, 1192, 1267, 1663
DraIII CACNNNGTG 1 cut(s) 1378
EaeI YGGCCR 4 cut(s) 447, 870, 1107, 1380
Eam1104I CTCTTC 1 cut(s) 1495
EarI CTCTTC 1 cut(s) 1495
EciI GGCGGA 1 cut(s) 1548
Ecl136II GAGCTC 2 cut(s) 237, 1058
Eco130I CCWWGG 2 cut(s) 1324, 1522
Eco24I GRGCYC 3 cut(s) 239, 423, 1060
Eco53kI GAGCTC 2 cut(s) 237, 1058
Eco57I CTGAAG 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 1582
EcoICRI GAGCTC 2 cut(s) 237, 1058
EcoO109I RGGNCCY 1 cut(s) 419
EcoRI GAATTC 2 cut(s) 504, 804
EcoRII CCWGG 6 cut(s) 114, 176, 301, 535, 1374, 1561
EcoT14I CCWWGG 2 cut(s) 1324, 1522
EcoT38I GRGCYC 3 cut(s) 239, 423, 1060
ErhI CCWWGG 2 cut(s) 1324, 1522
FaqI GGGAC 2 cut(s) 97, 659
FauI CCCGC 1 cut(s) 633
FauNDI CATATG 1 cut(s) 951
FokI GGATG 2 cut(s) 608, 837
FriOI GRGCYC 3 cut(s) 239, 423, 1060
FspBI CTAG 7 cut(s) 131, 159, 842, 893, 1020, 1053, 1473
GlaI GCGC 1 cut(s) 835
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 6 cut(s) 421, 449, 711, 872, 1109, 1382
HapII CCGG 3 cut(s) 1236, 1571, 1583
HhaI GCGC 1 cut(s) 836
Hin6I GCGC 1 cut(s) 834
HinP1I GCGC 1 cut(s) 834
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 4 cut(s) 344, 1168, 1462, 1605
HinfI GANTC 4 cut(s) 127, 1215, 1433, 1567
HpaII CCGG 3 cut(s) 1236, 1571, 1583
HphI GGTGA 1 cut(s) 1442
Hpy166II GTNNAC 2 cut(s) 465, 1286
Hpy188I TCNGA 1 cut(s) 1663
Hpy8I GTNNAC 2 cut(s) 465, 1286
HpyAV CCTTC 5 cut(s) 184, 256, 701, 1194, 1415
HpyCH4III ACNGT 1 cut(s) 1104
HpyCH4IV ACGT 1 cut(s) 768
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 521, 584, 833, 893, 1055, 1388, 1445
HpyF3I CTNAG 9 cut(s) 16, 216, 225, 342, 381, 612, 855, 1398, 1465
HpySE526I ACGT 1 cut(s) 768
HspAI GCGC 1 cut(s) 834
Kzo9I GATC 5 cut(s) 193, 391, 1192, 1267, 1663
LmnI GCTCC 3 cut(s) 242, 1540, 1655
LweI GCATC 6 cut(s) 29, 849, 1036, 1046, 1400, 1468
MabI ACCWGGT 2 cut(s) 176, 1374
MaeI CTAG 7 cut(s) 131, 159, 842, 893, 1020, 1053, 1473
MaeII ACGT 1 cut(s) 768
MaeIII GTNAC 3 cut(s) 661, 733, 1098
MalI GATC 5 cut(s) 195, 393, 1194, 1269, 1665
MboI GATC 5 cut(s) 193, 391, 1192, 1267, 1663
MboII GAAGA 4 cut(s) 324, 986, 1132, 1512
MhlI GDGCHC 4 cut(s) 239, 309, 423, 1060
MlsI TGGCCA 1 cut(s) 1109
MluNI TGGCCA 1 cut(s) 1109
MlyI GAGTC 1 cut(s) 1442
MmeI TCCRAC 2 cut(s) 234, 1243
Mox20I TGGCCA 1 cut(s) 1109
MroXI GAANNNNTTC 1 cut(s) 1138
MscI TGGCCA 1 cut(s) 1109
MseI TTAA 5 cut(s) 29, 338, 356, 548, 1320
MslI CAYNNNNRTG 2 cut(s) 798, 1115
Msp20I TGGCCA 1 cut(s) 1109
MspA1I CMGCKG 2 cut(s) 272, 722
MspI CCGG 3 cut(s) 1236, 1571, 1583
MspR9I CCNGG 8 cut(s) 116, 178, 303, 537, 1376, 1563, 1583, 1584
MvaI CCWGG 6 cut(s) 116, 178, 303, 537, 1376, 1563
MwoI GCNNNNNNNGC 8 cut(s) 263, 521, 584, 833, 893, 1055, 1388, 1445
NciI CCSGG 2 cut(s) 1583, 1584
NdeI CATATG 1 cut(s) 951
NdeII GATC 5 cut(s) 193, 391, 1192, 1267, 1663
NlaIV GGNNCC 4 cut(s) 421, 479, 493, 1330
NmuCI GTSAC 2 cut(s) 661, 733
NspI RCATGY 4 cut(s) 501, 531, 1346, 1628
PagI TCATGA 2 cut(s) 370, 1270
PasI CCCWGGG 1 cut(s) 302
PciI ACATGT 3 cut(s) 497, 527, 1624
PdmI GAANNNNTTC 1 cut(s) 1138
PfeI GAWTC 3 cut(s) 127, 1215, 1567
PleI GAGTC 1 cut(s) 1441
PpsI GAGTC 1 cut(s) 1441
PscI ACATGT 3 cut(s) 497, 527, 1624
PsiI TTATAA 1 cut(s) 633
Psp124BI GAGCTC 2 cut(s) 239, 1060
Psp6I CCWGG 6 cut(s) 114, 176, 301, 535, 1374, 1561
PspGI CCWGG 6 cut(s) 114, 176, 301, 535, 1374, 1561
PspN4I GGNNCC 4 cut(s) 421, 479, 493, 1330
PspOMI GGGCCC 1 cut(s) 419
PspPI GGNCC 2 cut(s) 419, 420
PstI CTGCAG 2 cut(s) 727, 1694
PvuII CAGCTG 2 cut(s) 272, 722
RsaI GTAC 3 cut(s) 609, 617, 1262
RsaNI GTAC 3 cut(s) 608, 616, 1261
RseI CAYNNNNRTG 2 cut(s) 798, 1115
SacI GAGCTC 2 cut(s) 239, 1060
SaqAI TTAA 5 cut(s) 29, 338, 356, 548, 1320
Sau3AI GATC 5 cut(s) 193, 391, 1192, 1267, 1663
Sau96I GGNCC 2 cut(s) 419, 420
SchI GAGTC 1 cut(s) 1442
ScrFI CCNGG 8 cut(s) 116, 178, 303, 537, 1376, 1563, 1583, 1584
SduI GDGCHC 4 cut(s) 239, 309, 423, 1060
SexAI ACCWGGT 2 cut(s) 176, 1374
SfaNI GCATC 6 cut(s) 29, 849, 1036, 1046, 1400, 1468
SfcI CTRYAG 3 cut(s) 723, 1333, 1690
SmaI CCCGGG 1 cut(s) 1584
SmiMI CAYNNNNRTG 2 cut(s) 798, 1115
SpeI ACTAGT 1 cut(s) 158
SsiI CCGC 6 cut(s) 96, 446, 626, 1201, 1383, 1559
SspI AATATT 1 cut(s) 1084
SspMI CTAG 7 cut(s) 131, 159, 842, 893, 1020, 1053, 1473
SstI GAGCTC 2 cut(s) 239, 1060
StyD4I CCNGG 8 cut(s) 114, 176, 301, 535, 1374, 1561, 1581, 1582
StyI CCWWGG 2 cut(s) 1324, 1522
TaaI ACNGT 1 cut(s) 1104
TaiI ACGT 1 cut(s) 771
TaqI TCGA 2 cut(s) 319, 1158
TatI WGTACW 2 cut(s) 615, 1260
TauI GCSGC 3 cut(s) 449, 1204, 1385
TfiI GAWTC 3 cut(s) 127, 1215, 1567
Tru1I TTAA 5 cut(s) 29, 338, 356, 548, 1320
Tru9I TTAA 5 cut(s) 29, 338, 356, 548, 1320
TscAI CASTG 3 cut(s) 252, 647, 1657
TseFI GTSAC 2 cut(s) 661, 733
Tsp45I GTSAC 2 cut(s) 661, 733
TspDTI ATGAA 7 cut(s) 35, 359, 839, 1257, 1266, 1287, 1542
TspMI CCCGGG 1 cut(s) 1582
TspRI CASTG 3 cut(s) 252, 647, 1657
XapI RAATTY 7 cut(s) 70, 504, 543, 804, 981, 1137, 1530
XbaI TCTAGA 1 cut(s) 130
XceI RCATGY 4 cut(s) 501, 531, 1346, 1628
XmaI CCCGGG 1 cut(s) 1582
XmnI GAANNNNTTC 1 cut(s) 1138
XspI CTAG 7 cut(s) 131, 159, 842, 893, 1020, 1053, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.