Rh4AG081100

Arginine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0015592
Physical Location & Seq
Forward (+)
16978838 .. 16982636
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG081100.1

Sequence Viewer

Length: 1686 bp
ATGTTCAGTGTTTCTGTGTTCTCAGCTTCCTCAGACCTCAGTTTTCATTTGGGTTATAGAAATAACTGGACTTTTGGAAGACCAAGTGCTTCTCTCTCAGAGGAAAGTAGCTATTTAGAGCCTAGAAAGAAGGAAGAGAAAACAAGTGGGAGAGTGACGGCCTGGTCTGCCACCGAGGATAAGATTTTACTTTCTAGCTCTCTGACAACTTCTCAGCAACAGAACCCGACTCCACTTGTTACCGCCTTAAAGGCTTCAGCTGAACAAAATGCTGCTACCTTTCACTTTCCTGGGCACAACAGAGGTCGAGCTGCACCTGATTCTATAACTGAACTTATTGGTCTAAAACCTTTTCTTCATGACTTACCTGAGCTTCCAGAACTTGACAACCTCTTTTCTCCGGAAGGCCCAATTTTAGATGCACAACAACAGGCAGCCAAACTCTTTGGCTCATTGGAGACATGGTTCCTTGTAGGAGGTACGACATGTGGAATTCAAGCAGCCATTATGGCTACTTGTTCCCCTGGAGATATTTTAGTTCTCCCTCGGAATTCCCATATATCAGCTGTATCTGCTTTAATATTGTCTGGTGCAGTACCCAAGTACATTATCCCTGATTATAATTGTGACTGGGACATTGCTGTTGGGGTCACTCCATCACAGGTGGAGACGGCTATCAAGGAAGTAGAGAAAGAAGGGCAAAAGGCAGCCGCTGTTTTCATCACTTCCCCAACATATCATGGTATATGCAGCAACTTGAGCGAGATAACCCAATTGTGCCATTCTCATGGTATTCCTGTAATTGTTGATGAGGCCCATGGGGCACATTTGGGATTTCATCCCCATATGCCGAATTCAGCCATGCAGCAAGGTGCTGATATAGCTGTCCAATCCACTCACAAGGTTCTTTGCTCGCTCACACAATCATCGATGTTGCACTTATCAGGCAGACTTGTAGATAGAGAAAAGATTTCTAGATGCCTTCAAACACTTCAAAGCACTAGTCCTAGTTATCTTCTTTTGGCATCATTAGATGCTGCTAGAGCTCAAATTAGTGAAGATCCCGAAACTATTTTTGAAAAAGCATTGCAACTGGCTATTGAAGCTAGGAATATGTTGAGAAAGACTAGAGGCATTTCAGTGCTTGATATTTCAAGCTTCCCTAAGTTCCCTGCAATTGATCCTTTGCGGCTTACTATAGGATTTCGACAGCTTGGCTTGTCTGGTTATGAAGCTGATGAAATTCTGTATGAGGATCATGATATCATTTGTGAACTTGTTGAGACCCAATGCATTACTTTTGTAATTACCCTTGGAACTTGCAGAGAGCATGTTCAGAAGCTTGTATCAGGCATAAAGCATCTAGCAGCCACTTTTGCATCGACCTGTGCTGATAAAAGGAAGGTGGACGGCAATAATCTTGCACCGTTTGCAGATATTAAAACTAGCTTGATTCCTAGAGATGCATTTTTTAGTGGTAAAAGGAGAGTGAGTGTCGAAAAGAGCTTGGGCGAAGTTTGTGGTGAGCTTATATGTCCATATCCACCTGGGATTCCAGTATTGATTCCTGGAGAGATTATCACAAAGAAAGCTTTGGATTATCTGCTACAAGTTAGAAGCAAGGGCGCTGTCATCACTGGAGCTTCTGATTCCCCACTTTCTTCCATAATTGTCTGCAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OKR_DC_1 OKR_DC_1_C

Protein Analysis

561

Amino Acids

60.74

Weight (kDa)

5.95

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OKR_DC_1 PF01276 77 - 383 6.8e-70 Orn/Lys/Arg decarboxylase, major domain
Aminotran_1_2 PF00155 140 - 357 1.8e-11 Aminotransferase class I and II
Beta_elim_lyase PF01212 140 - 303 7.2e-07 Beta-eliminating lyase
Aminotran_5 PF00266 154 - 303 7.4e-06 Aminotransferase class-V
DegT_DnrJ_EryC1 PF01041 161 - 275 2.2e-07 DegT/DnrJ/EryC1/StrS aminotransferase family
SepSecS PF05889 177 - 303 2.5e-06 O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS
OKR_DC_1_C PF03711 495 - 540 2.7e-08 Orn/Lys/Arg decarboxylase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015592)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06230 FvH4_4g06230 FvH4_4g06230
malus_domestica MD16G1239400.v1.1
prunus_persica Prupe.1G065500_v2.0.a1
pyrus_communis pycom16g20030
rosa_chinensis RchiOBHm_Chr4g0397951
rosa_laevigata RLG00000009394
rosa_multiflora Rmu_sc0000361.1_g000052 Rmu_sc0001074.1_g000007
rosa_roxburghii Rroxscaffold_5G00342850
rosa_rugosa Rorug04G0004800
rosa_samantha Rh4AG081100 Rh4DG074600
rosa_wichuraiana Rw0G020270 Rw4G006760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AasI GACNNNNNNGTC 1 cut(s) 163
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 3 cut(s) 243, 711, 1189
AclWI GGATC 3 cut(s) 1055, 1175, 1263
AcsI RAATTY 4 cut(s) 492, 550, 853, 1242
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 3 cut(s) 481, 597, 605
AflIII ACRYGT 1 cut(s) 485
AgsI TTSAA 6 cut(s) 497, 986, 995, 1079, 1103, 1155
AhlI ACTAGT 1 cut(s) 1001
AjnI CCWGG 5 cut(s) 161, 289, 523, 1546, 1567
AloI GAACNNNNNNTCC 2 cut(s) 449, 481
Alw21I GWGCWC 1 cut(s) 1048
Alw26I GTCTC 3 cut(s) 452, 662, 1277
AlwI GGATC 3 cut(s) 1055, 1175, 1263
AlwNI CAGNNNCTG 1 cut(s) 713
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 3 cut(s) 159, 406, 813
ApeKI GCWGC 9 cut(s) 272, 311, 434, 500, 707, 750, 865, 1037, 1367
ApoI RAATTY 4 cut(s) 492, 550, 853, 1242
ArsI GACNNNNNNTTYG 2 cut(s) 988, 1020
AspLEI GCGC 1 cut(s) 1628
AspS9I GGNCC 2 cut(s) 407, 814
AsuHPI GGTGA 1 cut(s) 1535
BaeGI GKGCMC 2 cut(s) 297, 826
BanII GRGCYC 1 cut(s) 1048
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 1048
BbvI GCAGC 9 cut(s) 259, 298, 446, 512, 719, 762, 877, 1024, 1379
BccI CCATC 1 cut(s) 664
BceAI ACGGC 3 cut(s) 174, 687, 1426
BciT130I CCWGG 5 cut(s) 163, 291, 525, 1548, 1569
BcoDI GTCTC 3 cut(s) 452, 662, 1277
BcuI ACTAGT 1 cut(s) 1001
BfmI CTRYAG 1 cut(s) 1197
BfoI RGCGCY 1 cut(s) 1629
BglI GCCNNNNNGGC 3 cut(s) 251, 509, 821
Bme1390I CCNGG 5 cut(s) 163, 291, 525, 1548, 1569
BmgT120I GGNCC 2 cut(s) 407, 814
BmiI GGNNCC 1 cut(s) 467
BmrFI CCNGG 5 cut(s) 163, 291, 525, 1548, 1569
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 7 cut(s) 409, 968, 1024, 1034, 1369, 1388, 1453
BmuI ACTGGG 1 cut(s) 640
BpiI GAAGAC 1 cut(s) 85
BpmI CTGGAG 3 cut(s) 546, 1590, 1659
Bpu10I CCTNAGC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 778
Bsa29I ATCGAT 1 cut(s) 929
BsaI GGTCTC 1 cut(s) 1277
BsaJI CCNNGG 7 cut(s) 174, 290, 523, 545, 817, 1312, 1547
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 4 cut(s) 449, 479, 1477, 1507
Bse1I ACTGG 5 cut(s) 71, 635, 1098, 1556, 1642
Bse3DI GCAATG 2 cut(s) 636, 1085
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 5 cut(s) 163, 291, 525, 1548, 1569
BseCI ATCGAT 1 cut(s) 929
BseDI CCNNGG 7 cut(s) 174, 290, 523, 545, 817, 1312, 1547
BseGI GGATG 1 cut(s) 838
BseMI GCAATG 2 cut(s) 636, 1085
BseMII CTCAG 6 cut(s) 36, 45, 52, 111, 227, 360
BseNI ACTGG 5 cut(s) 71, 635, 1098, 1556, 1642
BseSI GKGCMC 2 cut(s) 297, 826
BseXI GCAGC 9 cut(s) 259, 298, 446, 512, 719, 762, 877, 1024, 1379
BsgI GTGCAG 2 cut(s) 297, 612
BshFI GGCC 3 cut(s) 161, 408, 815
BshVI ATCGAT 1 cut(s) 929
BsiHKAI GWGCWC 1 cut(s) 1048
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 1 cut(s) 647
BsmAI GTCTC 3 cut(s) 452, 662, 1277
BsmBI CGTCTC 1 cut(s) 662
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 3 cut(s) 161, 408, 815
Bso31I GGTCTC 1 cut(s) 1277
Bsp1286I GDGCHC 3 cut(s) 297, 826, 1048
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 3 cut(s) 1060, 1180, 1255
Bsp19I CCATGG 1 cut(s) 817
BspACI CCGC 3 cut(s) 243, 711, 1189
BspANI GGCC 3 cut(s) 161, 408, 815
BspCNI CTCAG 6 cut(s) 35, 44, 51, 110, 226, 361
BspDI ATCGAT 1 cut(s) 929
BspEI TCCGGA 1 cut(s) 400
BspHI TCATGA 2 cut(s) 358, 1258
BspLI GGNNCC 1 cut(s) 467
BspPI GGATC 3 cut(s) 1055, 1175, 1263
BspTNI GGTCTC 1 cut(s) 1277
BsrDI GCAATG 2 cut(s) 636, 1085
BsrI ACTGG 5 cut(s) 71, 635, 1098, 1556, 1642
BssECI CCNNGG 7 cut(s) 174, 290, 523, 545, 817, 1312, 1547
BssMI GATC 3 cut(s) 1060, 1180, 1255
BssT1I CCWWGG 2 cut(s) 817, 1312
Bst2UI CCWGG 5 cut(s) 163, 291, 525, 1548, 1569
Bst4CI ACNGT 1 cut(s) 1428
Bst6I CTCTTC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 1430
BstC8I GCNNGC 1 cut(s) 914
BstDEI CTNAG 7 cut(s) 22, 31, 38, 97, 213, 369, 1164
BstDSI CCRYGG 1 cut(s) 817
BstF5I GGATG 1 cut(s) 838
BstH2I RGCGCY 1 cut(s) 1629
BstHHI GCGC 1 cut(s) 1628
BstKTI GATC 3 cut(s) 1063, 1183, 1258
BstMAI GTCTC 3 cut(s) 452, 662, 1277
BstMBI GATC 3 cut(s) 1060, 1180, 1255
BstNI CCWGG 5 cut(s) 163, 291, 525, 1548, 1569
BstNSI RCATGY 2 cut(s) 489, 1334
BstSCI CCNGG 5 cut(s) 161, 289, 523, 1546, 1567
BstSFI CTRYAG 1 cut(s) 1197
BstSLI GKGCMC 2 cut(s) 297, 826
BstV1I GCAGC 9 cut(s) 259, 298, 446, 512, 719, 762, 877, 1024, 1379
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 1 cut(s) 1060
BstXI CCANNNNNNTGG 1 cut(s) 788
BstYI RGATCY 1 cut(s) 1060
Bsu15I ATCGAT 1 cut(s) 929
BsuRI GGCC 3 cut(s) 161, 408, 815
BsuTUI ATCGAT 1 cut(s) 929
BtgI CCRYGG 1 cut(s) 817
BtsCI GGATG 1 cut(s) 838
BtsIMutI CAGTG 3 cut(s) 13, 1146, 1635
Cac8I GCNNGC 1 cut(s) 914
CaiI CAGNNNCTG 1 cut(s) 713
CciI TCATGA 2 cut(s) 358, 1258
CfoI GCGC 1 cut(s) 1628
Cfr13I GGNCC 2 cut(s) 407, 814
ClaI ATCGAT 1 cut(s) 929
Csp6I GTAC 3 cut(s) 480, 596, 604
CviAII CATG 9 cut(s) 359, 462, 486, 740, 788, 818, 862, 1259, 1331
CviQI GTAC 3 cut(s) 480, 596, 604
DdeI CTNAG 7 cut(s) 22, 31, 38, 97, 213, 369, 1164
DpnI GATC 3 cut(s) 1062, 1182, 1257
DpnII GATC 3 cut(s) 1060, 1180, 1255
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 1046
Eco130I CCWWGG 2 cut(s) 817, 1312
Eco24I GRGCYC 1 cut(s) 1048
Eco31I GGTCTC 1 cut(s) 1277
Eco32I GATATC 1 cut(s) 1264
Eco53kI GAGCTC 1 cut(s) 1046
Eco57I CTGAAG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 1046
EcoRI GAATTC 3 cut(s) 492, 550, 853
EcoRII CCWGG 5 cut(s) 161, 289, 523, 1546, 1567
EcoRV GATATC 1 cut(s) 1264
EcoT14I CCWWGG 2 cut(s) 817, 1312
EcoT22I ATGCAT 2 cut(s) 1295, 1468
EcoT38I GRGCYC 1 cut(s) 1048
ErhI CCWWGG 2 cut(s) 817, 1312
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 9 cut(s) 362, 465, 489, 743, 791, 821, 865, 1262, 1334
FaqI GGGAC 1 cut(s) 647
FatI CATG 9 cut(s) 358, 461, 485, 739, 787, 817, 861, 1258, 1330
FauNDI CATATG 1 cut(s) 846
FokI GGATG 1 cut(s) 825
FriOI GRGCYC 1 cut(s) 1048
GlaI GCGC 1 cut(s) 1627
GsuI CTGGAG 3 cut(s) 546, 1590, 1659
HaeII RGCGCY 1 cut(s) 1629
HaeIII GGCC 3 cut(s) 161, 408, 815
HapII CCGG 1 cut(s) 401
HhaI GCGC 1 cut(s) 1628
Hin1II CATG 9 cut(s) 362, 465, 489, 743, 791, 821, 865, 1262, 1334
Hin6I GCGC 1 cut(s) 1626
HinP1I GCGC 1 cut(s) 1626
HindIII AAGCTT 3 cut(s) 1156, 1340, 1590
HinfI GANTC 6 cut(s) 229, 320, 1453, 1552, 1564, 1649
HpaII CCGG 1 cut(s) 401
HphI GGTGA 1 cut(s) 1535
Hpy166II GTNNAC 2 cut(s) 1274, 1408
Hpy188I TCNGA 6 cut(s) 34, 100, 204, 549, 1338, 1648
Hpy188III TCNNGA 6 cut(s) 359, 377, 401, 975, 1064, 1259
Hpy8I GTNNAC 2 cut(s) 1274, 1408
HpyAV CCTTC 5 cut(s) 124, 398, 689, 992, 1396
HpyCH4III ACNGT 1 cut(s) 1428
HpyF3I CTNAG 7 cut(s) 22, 31, 38, 97, 213, 369, 1164
Hsp92II CATG 9 cut(s) 362, 465, 489, 743, 791, 821, 865, 1262, 1334
HspAI GCGC 1 cut(s) 1626
Kpn2I TCCGGA 1 cut(s) 400
Kzo9I GATC 3 cut(s) 1060, 1180, 1255
LmnI GCTCC 1 cut(s) 1640
Lsp1109I GCAGC 9 cut(s) 259, 298, 446, 512, 719, 762, 877, 1024, 1379
LweI GCATC 7 cut(s) 409, 968, 1024, 1034, 1369, 1388, 1453
MaeIII GTNAC 4 cut(s) 154, 238, 626, 649
MalI GATC 3 cut(s) 1062, 1182, 1257
MboI GATC 3 cut(s) 1060, 1180, 1255
MboII GAAGA 6 cut(s) 90, 146, 347, 1007, 1070, 1653
MfeI CAATTG 2 cut(s) 773, 1176
MflI RGATCY 1 cut(s) 1060
MhlI GDGCHC 3 cut(s) 297, 826, 1048
MlyI GAGTC 1 cut(s) 223
Mph1103I ATGCAT 2 cut(s) 1295, 1468
MroI TCCGGA 1 cut(s) 400
MseI TTAA 3 cut(s) 248, 578, 1440
MslI CAYNNNNRTG 2 cut(s) 786, 1139
MspA1I CMGCKG 3 cut(s) 260, 566, 713
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 5 cut(s) 163, 291, 525, 1548, 1569
MunI CAATTG 2 cut(s) 773, 1176
MvaI CCWGG 5 cut(s) 163, 291, 525, 1548, 1569
NcoI CCATGG 1 cut(s) 817
NdeI CATATG 1 cut(s) 846
NdeII GATC 3 cut(s) 1060, 1180, 1255
NlaIII CATG 9 cut(s) 362, 465, 489, 743, 791, 821, 865, 1262, 1334
NlaIV GGNNCC 1 cut(s) 467
NmuCI GTSAC 3 cut(s) 154, 626, 649
NsiI ATGCAT 2 cut(s) 1295, 1468
NspI RCATGY 2 cut(s) 489, 1334
PagI TCATGA 2 cut(s) 358, 1258
PciI ACATGT 1 cut(s) 485
PfeI GAWTC 5 cut(s) 320, 1453, 1552, 1564, 1649
PfoI TCCNGGA 1 cut(s) 1567
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PscI ACATGT 1 cut(s) 485
PsiI TTATAA 1 cut(s) 621
Psp124BI GAGCTC 1 cut(s) 1048
Psp6I CCWGG 5 cut(s) 161, 289, 523, 1546, 1567
PspGI CCWGG 5 cut(s) 161, 289, 523, 1546, 1567
PspN4I GGNNCC 1 cut(s) 467
PspPI GGNCC 2 cut(s) 407, 814
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 1 cut(s) 1060
PvuII CAGCTG 2 cut(s) 260, 566
RsaI GTAC 3 cut(s) 481, 597, 605
RsaNI GTAC 3 cut(s) 480, 596, 604
RseI CAYNNNNRTG 2 cut(s) 786, 1139
SacI GAGCTC 1 cut(s) 1048
SaqAI TTAA 3 cut(s) 248, 578, 1440
Sau3AI GATC 3 cut(s) 1060, 1180, 1255
Sau96I GGNCC 2 cut(s) 407, 814
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 5 cut(s) 163, 291, 525, 1548, 1569
SduI GDGCHC 3 cut(s) 297, 826, 1048
SfaNI GCATC 7 cut(s) 409, 968, 1024, 1034, 1369, 1388, 1453
SfcI CTRYAG 1 cut(s) 1197
SmiMI CAYNNNNRTG 2 cut(s) 786, 1139
SmlI CTYRAG 1 cut(s) 757
SmoI CTYRAG 1 cut(s) 757
SpeI ACTAGT 1 cut(s) 1001
SsiI CCGC 3 cut(s) 243, 711, 1189
SspI AATATT 1 cut(s) 582
SstI GAGCTC 1 cut(s) 1048
StyD4I CCNGG 5 cut(s) 161, 289, 523, 1546, 1567
StyI CCWWGG 2 cut(s) 817, 1312
TaaI ACNGT 1 cut(s) 1428
TaqI TCGA 5 cut(s) 307, 929, 1207, 1382, 1497
TatI WGTACW 1 cut(s) 603
TauI GCSGC 2 cut(s) 713, 1192
TfiI GAWTC 5 cut(s) 320, 1453, 1552, 1564, 1649
Tru1I TTAA 3 cut(s) 248, 578, 1440
Tru9I TTAA 3 cut(s) 248, 578, 1440
TscAI CASTG 3 cut(s) 13, 1146, 1642
TseFI GTSAC 3 cut(s) 154, 626, 649
TseI GCWGC 9 cut(s) 272, 311, 434, 500, 707, 750, 865, 1037, 1367
Tsp45I GTSAC 3 cut(s) 154, 626, 649
TspDTI ATGAA 6 cut(s) 35, 347, 709, 827, 1245, 1254
TspRI CASTG 3 cut(s) 13, 1146, 1642
XapI RAATTY 4 cut(s) 492, 550, 853, 1242
XbaI TCTAGA 1 cut(s) 974
XceI RCATGY 2 cut(s) 489, 1334
Zsp2I ATGCAT 2 cut(s) 1295, 1468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.