FvH4_4g08220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015341
Physical Location & Seq
Forward (+)
8078809 .. 8080083
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08220.t1

Sequence Viewer

Length: 810 bp
ATGGGAGACGACAATATCAACAACGCTGATTTTCAATTTCAACAAGACTCCTACGAAGACGAAGAAGCCGAGGAGGCACTTTCTCTCTGCGACCTTCCACTGGACAACGAAGCTCAAGAGTTCGACGACTTGTCGACCAAGTTCCTAAAGGCTCGCTGCTCTTCCTCTGAGTTCTTCGAATTCTTCAGCGACCACAGCTCCGATAGCTTCATGTGTTCCGCCGAGGACATCATCGTCTGTGGGAAACTCATACCCTTCAAAGAGAACACACCTACTCCTCAAGCCCCGAAGCTTTCTTCCAATCCCAAGCATGATCATCAGAAGAAACAAACAGCCTTCAGACGACGGTCCGAATCACTTTCCGGGATAGAAAGCCCCGTCACGAGATCAAGCAGCACTAAAAACAAGATCATGATGACCAACAGCCGATCTTTGGATTACAGGAAGCTTCACCGCCAGAGCTCGATGGTGTCACCGGTGCCGGAGATGGAGCGTAATTCGTCGGTGAGGAGTGTGGGATCATCAAAGTCCGATAAGAAGAGTACTGGTAGTGGTAAGCCAAAGTGGTCATTTCTCTTGTTTGGGATCGTCAAGTTTCCGGCGGAGATGGATCTCAGCGACATCAAAAGCCGGCAGGTGAGGAAAAACCCGTCTACGATTCTGTTTCCTGATACTTCCAGCGGGAAGTGTTCTTCGGTCAGTCGGAGCAACTCCGGTAACAAGGGGTCGTGGAAGCTGCTCAGGGCATTGAGCTGCAAGGACCATGCGAGTGTTTCCGTAACGACGTCGTTTTACGTCTCACAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

270

Amino Acids

30.02

Weight (kDa)

8.15

Isoelectric Point (pI)

57.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30230
fragaria_vesca FvH4_4g08220
malus_domestica MD13G1253900.v1.1 MD16G1257900.v1.1
prunus_persica Prupe.1G086100_v2.0.a1
pyrus_communis pycom13g22430
rosa_chinensis RchiOBHm_Chr4g0402471
rosa_laevigata RLG00000009081
rosa_multiflora Rmu_co8180070.1_g000001 Rmu_ssc0000330.1_g000029
rosa_roxburghii Rroxscaffold_5G00347160
rosa_rugosa Rorug04G0035700
rosa_samantha Rh4BG105500 Rh4CG119900 Rh4DG104900
rosa_wichuraiana Rw4G009060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 625
AasI GACNNNNNNGTC 1 cut(s) 233
AatII GACGTC 1 cut(s) 788
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 478
AccI GTMKAC 2 cut(s) 134, 653
AciI CCGC 4 cut(s) 219, 454, 602, 681
AclWI GGATC 3 cut(s) 526, 593, 618
AcsI RAATTY 1 cut(s) 179
AcuI CTGAAG 2 cut(s) 169, 322
AcyI GRCGYC 1 cut(s) 785
AfaI GTAC 1 cut(s) 544
AfiI CCNNNNNNNGG 2 cut(s) 100, 433
AgeI ACCGGT 1 cut(s) 475
AgsI TTSAA 3 cut(s) 35, 41, 259
AhdI GACNNNNNGTC 1 cut(s) 130
AluBI AGCT 8 cut(s) 113, 198, 207, 292, 448, 462, 736, 753
AluI AGCT 8 cut(s) 113, 198, 207, 292, 448, 462, 736, 753
Alw21I GWGCWC 1 cut(s) 464
Alw26I GTCTC 1 cut(s) 802
AlwI GGATC 3 cut(s) 526, 593, 618
ApeKI GCWGC 4 cut(s) 156, 393, 736, 753
ApoI RAATTY 1 cut(s) 179
AsiGI ACCGGT 1 cut(s) 475
AspS9I GGNCC 2 cut(s) 348, 760
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 4 cut(s) 443, 465, 517, 649
AsuII TTCGAA 1 cut(s) 177
AvaII GGWCC 2 cut(s) 348, 760
BanI GGYRCC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 464
BauI CACGAG 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 464
BbvI GCAGC 4 cut(s) 143, 405, 723, 740
BccI CCATC 3 cut(s) 460, 481, 601
BclI TGATCA 1 cut(s) 313
BcnI CCSGG 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 802
BfuAI ACCTGC 1 cut(s) 625
BglI GCCNNNNNGGC 1 cut(s) 74
BisI GCNGC 4 cut(s) 157, 394, 737, 754
BlsI GCNGC 4 cut(s) 158, 395, 738, 755
BmcAI AGTACT 1 cut(s) 544
Bme1390I CCNGG 1 cut(s) 364
Bme18I GGWCC 2 cut(s) 348, 760
BmeRI GACNNNNNGTC 1 cut(s) 130
BmgT120I GGNCC 2 cut(s) 348, 760
BmiI GGNNCC 1 cut(s) 480
BmrFI CCNGG 1 cut(s) 364
BoxI GACNNNNGTC 1 cut(s) 346
BpiI GAAGAC 1 cut(s) 63
Bpu10I CCTNAGC 1 cut(s) 740
Bpu14I TTCGAA 1 cut(s) 177
BpuEI CTTGAG 2 cut(s) 99, 264
BpuMI CCSGG 1 cut(s) 364
BsaHI GRCGYC 1 cut(s) 785
BsaJI CCNNGG 2 cut(s) 69, 222
BsaWI WCCGGW 2 cut(s) 475, 713
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 259, 289
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 433
Bse118I RCCGGY 2 cut(s) 475, 630
Bse1I ACTGG 2 cut(s) 105, 550
BseDI CCNNGG 2 cut(s) 69, 222
BseLI CCNNNNNNNGG 2 cut(s) 100, 433
BseMII CTCAG 3 cut(s) 159, 628, 754
BseNI ACTGG 2 cut(s) 105, 550
BseRI GAGGAG 3 cut(s) 86, 267, 523
BseXI GCAGC 4 cut(s) 143, 405, 723, 740
BshNI GGYRCC 1 cut(s) 478
BshTI ACCGGT 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 464
BsiSI CCGG 6 cut(s) 363, 476, 482, 599, 631, 714
BslI CCNNNNNNNGG 2 cut(s) 100, 433
BsmAI GTCTC 1 cut(s) 802
BsmBI CGTCTC 1 cut(s) 802
Bsp119I TTCGAA 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 464
Bsp143I GATC 7 cut(s) 313, 386, 408, 428, 518, 585, 610
BspACI CCGC 4 cut(s) 219, 454, 602, 681
BspCNI CTCAG 3 cut(s) 160, 627, 753
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 1 cut(s) 480
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 3 cut(s) 526, 593, 618
BspQI GCTCTTC 1 cut(s) 166
BspT104I TTCGAA 1 cut(s) 177
BspT107I GGYRCC 1 cut(s) 478
BsrFI RCCGGY 2 cut(s) 475, 630
BsrI ACTGG 2 cut(s) 105, 550
BssAI RCCGGY 2 cut(s) 475, 630
BssECI CCNNGG 2 cut(s) 69, 222
BssMI GATC 7 cut(s) 313, 386, 408, 428, 518, 585, 610
BssNI GRCGYC 1 cut(s) 785
BssSI CACGAG 1 cut(s) 382
Bst2BI CACGAG 1 cut(s) 382
Bst4CI ACNGT 1 cut(s) 348
Bst6I CTCTTC 2 cut(s) 166, 533
BstACI GRCGYC 1 cut(s) 785
BstBI TTCGAA 1 cut(s) 177
BstC8I GCNNGC 2 cut(s) 154, 632
BstDEI CTNAG 3 cut(s) 168, 614, 740
BstKTI GATC 7 cut(s) 316, 389, 411, 431, 521, 588, 613
BstMAI GTCTC 1 cut(s) 802
BstMBI GATC 7 cut(s) 313, 386, 408, 428, 518, 585, 610
BstMWI GCNNNNNNNGC 3 cut(s) 74, 195, 204
BstPAI GACNNNNGTC 1 cut(s) 346
BstSCI CCNGG 1 cut(s) 362
BstV1I GCAGC 4 cut(s) 143, 405, 723, 740
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BtsIMutI CAGTG 1 cut(s) 98
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 2 cut(s) 154, 632
CciI TCATGA 1 cut(s) 411
Cfr10I RCCGGY 2 cut(s) 475, 630
Cfr13I GGNCC 2 cut(s) 348, 760
CpoI CGGWCCG 1 cut(s) 348
Csp6I GTAC 1 cut(s) 543
CspAI ACCGGT 1 cut(s) 475
CspI CGGWCCG 1 cut(s) 348
CviAII CATG 4 cut(s) 211, 311, 412, 764
CviQI GTAC 1 cut(s) 543
DdeI CTNAG 3 cut(s) 168, 614, 740
DpnI GATC 7 cut(s) 315, 388, 410, 430, 520, 587, 612
DpnII GATC 7 cut(s) 313, 386, 408, 428, 518, 585, 610
DrdI GACNNNNNNGTC 1 cut(s) 233
DriI GACNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 233
Eam1104I CTCTTC 2 cut(s) 166, 533
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 2 cut(s) 166, 533
EciI GGCGGA 2 cut(s) 208, 617
Ecl136II GAGCTC 1 cut(s) 462
Eco24I GRGCYC 1 cut(s) 464
Eco47I GGWCC 2 cut(s) 348, 760
Eco53kI GAGCTC 1 cut(s) 462
Eco57I CTGAAG 2 cut(s) 169, 322
EcoICRI GAGCTC 1 cut(s) 462
EcoRI GAATTC 1 cut(s) 179
EcoT38I GRGCYC 1 cut(s) 464
Esp3I CGTCTC 1 cut(s) 802
FaeI CATG 4 cut(s) 214, 314, 415, 767
FaiI YATR 6 cut(s) 212, 251, 312, 413, 765, 808
FatI CATG 4 cut(s) 210, 310, 411, 763
FauI CCCGC 1 cut(s) 674
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 2 cut(s) 134, 653
Fnu4HI GCNGC 4 cut(s) 157, 394, 737, 754
FriOI GRGCYC 1 cut(s) 464
Fsp4HI GCNGC 4 cut(s) 157, 394, 737, 754
GluI GCNGC 4 cut(s) 157, 394, 737, 754
HapII CCGG 6 cut(s) 363, 476, 482, 599, 631, 714
Hin1I GRCGYC 1 cut(s) 785
Hin1II CATG 4 cut(s) 214, 314, 415, 767
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HindIII AAGCTT 2 cut(s) 290, 446
HinfI GANTC 3 cut(s) 47, 353, 658
HpaII CCGG 6 cut(s) 363, 476, 482, 599, 631, 714
HphI GGTGA 4 cut(s) 443, 465, 517, 649
Hpy166II GTNNAC 2 cut(s) 135, 654
Hpy188I TCNGA 7 cut(s) 169, 202, 321, 341, 352, 532, 705
Hpy188III TCNNGA 4 cut(s) 116, 382, 412, 668
Hpy8I GTNNAC 2 cut(s) 135, 654
Hpy99I CGWCG 5 cut(s) 128, 348, 505, 787, 790
HpyAV CCTTC 3 cut(s) 104, 265, 346
HpyCH4III ACNGT 1 cut(s) 348
HpyCH4IV ACGT 2 cut(s) 785, 795
HpyCH4V TGCA 1 cut(s) 756
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 195, 204
HpyF3I CTNAG 3 cut(s) 168, 614, 740
HpySE526I ACGT 2 cut(s) 785, 795
Hsp92I GRCGYC 1 cut(s) 785
Hsp92II CATG 4 cut(s) 214, 314, 415, 767
KroI GCCGGC 1 cut(s) 630
KroNI GCCGGC 1 cut(s) 632
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 7 cut(s) 313, 386, 408, 428, 518, 585, 610
LguI GCTCTTC 1 cut(s) 166
LmnI GCTCC 3 cut(s) 203, 490, 705
Lsp1109I GCAGC 4 cut(s) 143, 405, 723, 740
MaeII ACGT 2 cut(s) 785, 795
MaeIII GTNAC 4 cut(s) 379, 471, 716, 778
MalI GATC 7 cut(s) 315, 388, 410, 430, 520, 587, 612
MboI GATC 7 cut(s) 313, 386, 408, 428, 518, 585, 610
MboII GAAGA 9 cut(s) 68, 74, 153, 166, 175, 288, 334, 550, 684
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 1 cut(s) 464
MluCI AATT 3 cut(s) 35, 179, 496
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 683
MnlI CCTC 7 cut(s) 64, 67, 175, 217, 288, 501, 633
MroNI GCCGGC 1 cut(s) 630
MslI CAYNNNNRTG 2 cut(s) 768, 805
MspA1I CMGCKG 1 cut(s) 681
MspI CCGG 6 cut(s) 363, 476, 482, 599, 631, 714
MspR9I CCNGG 1 cut(s) 364
MwoI GCNNNNNNNGC 3 cut(s) 74, 195, 204
NaeI GCCGGC 1 cut(s) 632
NciI CCSGG 1 cut(s) 364
NdeII GATC 7 cut(s) 313, 386, 408, 428, 518, 585, 610
NgoMIV GCCGGC 1 cut(s) 630
NlaIII CATG 4 cut(s) 214, 314, 415, 767
NlaIV GGNNCC 1 cut(s) 480
NmeAIII GCCGAG 2 cut(s) 94, 247
NmuCI GTSAC 2 cut(s) 379, 471
NspV TTCGAA 1 cut(s) 177
PagI TCATGA 1 cut(s) 411
PaqCI CACCTGC 1 cut(s) 625
PciSI GCTCTTC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdiI GCCGGC 1 cut(s) 632
PfeI GAWTC 2 cut(s) 353, 658
PfoI TCCNGGA 1 cut(s) 362
PinAI ACCGGT 1 cut(s) 475
PkrI GCNGC 4 cut(s) 158, 395, 738, 755
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PshAI GACNNNNGTC 1 cut(s) 346
Psp124BI GAGCTC 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 480
PspPI GGNCC 2 cut(s) 348, 760
PsuI RGATCY 1 cut(s) 610
RsaI GTAC 1 cut(s) 544
RsaNI GTAC 1 cut(s) 543
RseI CAYNNNNRTG 2 cut(s) 768, 805
Rsr2I CGGWCCG 1 cut(s) 348
RsrII CGGWCCG 1 cut(s) 348
SacI GAGCTC 1 cut(s) 464
SalI GTCGAC 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 166
SatI GCNGC 4 cut(s) 157, 394, 737, 754
Sau3AI GATC 7 cut(s) 313, 386, 408, 428, 518, 585, 610
Sau96I GGNCC 2 cut(s) 348, 760
ScaI AGTACT 1 cut(s) 544
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 364
SduI GDGCHC 1 cut(s) 464
SfuI TTCGAA 1 cut(s) 177
SgrAI CRCCGGYG 1 cut(s) 475
SinI GGWCC 2 cut(s) 348, 760
SmiMI CAYNNNNRTG 2 cut(s) 768, 805
SmlI CTYRAG 2 cut(s) 114, 279
SmoI CTYRAG 2 cut(s) 114, 279
Sse9I AATT 3 cut(s) 35, 179, 496
SsiI CCGC 4 cut(s) 219, 454, 602, 681
SstI GAGCTC 1 cut(s) 464
StyD4I CCNGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 348
TaiI ACGT 2 cut(s) 788, 798
TaqI TCGA 4 cut(s) 123, 134, 177, 464
TaqII GACCGA 1 cut(s) 685
TasI AATT 3 cut(s) 35, 179, 496
TatI WGTACW 1 cut(s) 542
TfiI GAWTC 2 cut(s) 353, 658
TscAI CASTG 1 cut(s) 105
TseFI GTSAC 2 cut(s) 379, 471
TseI GCWGC 4 cut(s) 156, 393, 736, 753
Tsp45I GTSAC 2 cut(s) 379, 471
TspDTI ATGAA 1 cut(s) 199
TspGWI ACGGA 1 cut(s) 766
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 2 cut(s) 348, 760
XapI RAATTY 1 cut(s) 179
XmiI GTMKAC 2 cut(s) 134, 653
ZraI GACGTC 1 cut(s) 786
ZrmI AGTACT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.