MD16G1257900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015341
Physical Location & Seq
Forward (+)
30341656 .. 30342435
780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1257900.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGAGGACAAAAATTACATTGAGAATATTCTTTGCTGTGGAGATCAATCAGAAGAAGAAGAAGAAGATGCAGAAGAAGCACTCTCTCTCTGCGACCTTCCGCTGGACAATATCCATGATCAAGTCAATGAATTCCACGACATGTCCTCCAAGCACCTACACGCTCGTCGTTCTTCCGCTGATCAGTTTTTCGAGTTCTTCAGCGAGTTCAGCACCGACAGCTTCATGTGTTCGGCCGAAGACATCATCGTCTGTGGGAAACTCATACCCTTCAAAGATCACGCAAATGAAGCCCCTCAGAAGACCTGTACTAACAATGGTTATCACAAAAAAAATTCATCTTTCCGAAGGAGGTCGGAGTCACTGCCCGGGTTACAAAGCTCGGTAGCAAGATCAAGCAGCACAAAGAATCAGATCATGATGAGAAACAGCAGATCATTGGATTATCAAAAGCTTCACGGGAAATCCTCGATGGTCTCCCCGACCCCCGCAGAGATGGAGAGGATTTCTTCCGTGAAGAGTGTTGGAAAATACGATAAGAAGTGTGGTAACAAGCCAAAGTGGTTTTTACTCATGTTTGGGATTGTGAGGTTTCAGGCTGAGTTGGACCTCAGCGATATCAAGAACCGGCAGATTCGACGAAACCCTTCCACGCTCTTCCGCAGTGAAGCCCCTGACAATCGGAGCTCCGGTAAGGGCTCTTGGAAACTGCTCAAGGTATTGAGCTGCAAGGACCATGCAAGTGTTGCTGCAAAGATGCCGCACTGTACACAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

29.4

Weight (kDa)

7.59

Isoelectric Point (pI)

70.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30230
fragaria_vesca FvH4_4g08220
malus_domestica MD13G1253900.v1.1 MD16G1257900.v1.1
prunus_persica Prupe.1G086100_v2.0.a1
pyrus_communis pycom13g22430
rosa_chinensis RchiOBHm_Chr4g0402471
rosa_laevigata RLG00000009081
rosa_multiflora Rmu_co8180070.1_g000001 Rmu_ssc0000330.1_g000029
rosa_roxburghii Rroxscaffold_5G00347160
rosa_rugosa Rorug04G0035700
rosa_samantha Rh4BG105500 Rh4CG119900 Rh4DG104900
rosa_wichuraiana Rw4G009060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AciI CCGC 5 cut(s) 101, 177, 489, 661, 761
AcoI YGGCCR 1 cut(s) 234
AcsI RAATTY 2 cut(s) 131, 334
AcuI CTGAAG 1 cut(s) 184
AfaI GTAC 2 cut(s) 310, 769
AfiI CCNNNNNNNGG 1 cut(s) 103
AflIII ACRYGT 1 cut(s) 141
AgsI TTSAA 1 cut(s) 274
AleI CACNNNNGTG 1 cut(s) 775
AluBI AGCT 5 cut(s) 222, 381, 454, 687, 726
AluI AGCT 5 cut(s) 222, 381, 454, 687, 726
Alw21I GWGCWC 1 cut(s) 689
Alw26I GTCTC 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 3 cut(s) 399, 726, 749
ApoI RAATTY 2 cut(s) 131, 334
Asp700I GAANNNNTTC 1 cut(s) 646
AspS9I GGNCC 2 cut(s) 607, 733
AsuC2I CCSGG 2 cut(s) 369, 370
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 2 cut(s) 607, 733
BanII GRGCYC 2 cut(s) 689, 701
BbsI GAAGAC 2 cut(s) 246, 308
Bbv12I GWGCWC 1 cut(s) 689
BbvCI CCTCAGC 1 cut(s) 611
BbvI GCAGC 3 cut(s) 411, 713, 736
BccI CCATC 2 cut(s) 466, 490
BclI TGATCA 2 cut(s) 118, 181
BcnI CCSGG 2 cut(s) 369, 370
BcoDI GTCTC 1 cut(s) 481
BisI GCNGC 4 cut(s) 400, 727, 750, 761
BlsI GCNGC 4 cut(s) 401, 728, 751, 762
Bme1390I CCNGG 2 cut(s) 369, 370
Bme18I GGWCC 2 cut(s) 607, 733
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 607, 733
BmrFI CCNGG 2 cut(s) 369, 370
BmsI GCATC 2 cut(s) 58, 747
BpiI GAAGAC 2 cut(s) 246, 308
Bpu10I CCTNAGC 1 cut(s) 611
BpuEI CTTGAG 1 cut(s) 698
BpuMI CCSGG 2 cut(s) 369, 370
BsaI GGTCTC 1 cut(s) 481
BsaJI CCNNGG 1 cut(s) 368
BsaWI WCCGGW 1 cut(s) 689
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 676, 706
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse118I RCCGGY 1 cut(s) 627
BseDI CCNNGG 1 cut(s) 368
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 3 cut(s) 311, 591, 625
BseX3I CGGCCG 1 cut(s) 234
BseXI GCAGC 3 cut(s) 411, 713, 736
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 1 cut(s) 236
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 689
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 3 cut(s) 369, 628, 690
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 481
BsnI GGCC 1 cut(s) 236
Bso31I GGTCTC 1 cut(s) 481
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 689, 701
Bsp1407I TGTACA 1 cut(s) 767
Bsp143I GATC 7 cut(s) 43, 118, 181, 277, 392, 414, 434
BspACI CCGC 5 cut(s) 101, 177, 489, 661, 761
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 3 cut(s) 310, 592, 624
BspHI TCATGA 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 481
BsrFI RCCGGY 1 cut(s) 627
BsrGI TGTACA 1 cut(s) 767
BssAI RCCGGY 1 cut(s) 627
BssECI CCNNGG 1 cut(s) 368
BssMI GATC 7 cut(s) 43, 118, 181, 277, 392, 414, 434
Bst4CI ACNGT 1 cut(s) 767
Bst6I CTCTTC 2 cut(s) 512, 662
BstAPI GCANNNNNTGC 1 cut(s) 746
BstAUI TGTACA 1 cut(s) 767
BstDEI CTNAG 3 cut(s) 297, 600, 611
BstKTI GATC 7 cut(s) 46, 121, 184, 280, 395, 417, 437
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 7 cut(s) 43, 118, 181, 277, 392, 414, 434
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 5 cut(s) 77, 210, 219, 290, 746
BstNSI RCATGY 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 367, 368
BstV1I GCAGC 3 cut(s) 411, 713, 736
BstV2I GAAGAC 2 cut(s) 246, 308
BstZI CGGCCG 1 cut(s) 234
BsuRI GGCC 1 cut(s) 236
BtsI GCAGTG 2 cut(s) 362, 670
BtsIMutI CAGTG 3 cut(s) 362, 670, 763
CciI TCATGA 1 cut(s) 417
Cfr10I RCCGGY 1 cut(s) 627
Cfr13I GGNCC 2 cut(s) 607, 733
Cfr9I CCCGGG 1 cut(s) 368
Csp6I GTAC 2 cut(s) 309, 768
CviAII CATG 6 cut(s) 116, 142, 226, 418, 574, 737
CviQI GTAC 2 cut(s) 309, 768
DdeI CTNAG 3 cut(s) 297, 600, 611
DpnI GATC 7 cut(s) 45, 120, 183, 279, 394, 416, 436
DpnII GATC 7 cut(s) 43, 118, 181, 277, 392, 414, 434
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 234
EagI CGGCCG 1 cut(s) 234
Eam1104I CTCTTC 2 cut(s) 512, 662
EarI CTCTTC 2 cut(s) 512, 662
Ecl136II GAGCTC 1 cut(s) 687
EclXI CGGCCG 1 cut(s) 234
Eco24I GRGCYC 2 cut(s) 689, 701
Eco31I GGTCTC 1 cut(s) 481
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 2 cut(s) 607, 733
Eco52I CGGCCG 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 687
Eco57I CTGAAG 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 687
EcoRI GAATTC 1 cut(s) 131
EcoRV GATATC 1 cut(s) 619
EcoT38I GRGCYC 2 cut(s) 689, 701
FaeI CATG 6 cut(s) 119, 145, 229, 421, 577, 740
FaiI YATR 7 cut(s) 117, 143, 227, 266, 419, 575, 738
FatI CATG 6 cut(s) 115, 141, 225, 417, 573, 736
FauI CCCGC 1 cut(s) 496
FbaI TGATCA 2 cut(s) 118, 181
Fnu4HI GCNGC 4 cut(s) 400, 727, 750, 761
FriOI GRGCYC 2 cut(s) 689, 701
Fsp4HI GCNGC 4 cut(s) 400, 727, 750, 761
GluI GCNGC 4 cut(s) 400, 727, 750, 761
HaeIII GGCC 1 cut(s) 236
HapII CCGG 3 cut(s) 369, 628, 690
Hin1II CATG 6 cut(s) 119, 145, 229, 421, 577, 740
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 359, 409, 634
HpaII CCGG 3 cut(s) 369, 628, 690
Hpy166II GTNNAC 1 cut(s) 770
Hpy188I TCNGA 6 cut(s) 52, 300, 347, 358, 414, 684
Hpy188III TCNNGA 2 cut(s) 418, 622
Hpy8I GTNNAC 1 cut(s) 770
Hpy99I CGWCG 2 cut(s) 171, 642
HpyAV CCTTC 4 cut(s) 107, 280, 342, 657
HpyCH4III ACNGT 1 cut(s) 767
HpyCH4V TGCA 4 cut(s) 71, 729, 740, 752
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 210, 219, 290, 746
HpyF3I CTNAG 3 cut(s) 297, 600, 611
Hsp92II CATG 6 cut(s) 119, 145, 229, 421, 577, 740
Ksp22I TGATCA 2 cut(s) 118, 181
Kzo9I GATC 7 cut(s) 43, 118, 181, 277, 392, 414, 434
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 2 cut(s) 684, 692
LpnPI CCDG 7 cut(s) 89, 319, 382, 581, 641, 687, 703
Lsp1109I GCAGC 3 cut(s) 411, 713, 736
LweI GCATC 2 cut(s) 58, 747
MaeIII GTNAC 3 cut(s) 360, 372, 548
MalI GATC 7 cut(s) 45, 120, 183, 279, 394, 416, 436
MboI GATC 7 cut(s) 43, 118, 181, 277, 392, 414, 434
MhlI GDGCHC 2 cut(s) 689, 701
MluCI AATT 3 cut(s) 13, 131, 334
MlyI GAGTC 1 cut(s) 368
MmeI TCCRAC 3 cut(s) 336, 505, 585
MnlI CCTC 7 cut(s) 157, 306, 345, 478, 495, 582, 620
MroXI GAANNNNTTC 1 cut(s) 646
MslI CAYNNNNRTG 3 cut(s) 285, 741, 775
MspA1I CMGCKG 2 cut(s) 103, 179
MspI CCGG 3 cut(s) 369, 628, 690
MspR9I CCNGG 2 cut(s) 369, 370
MwoI GCNNNNNNNGC 5 cut(s) 77, 210, 219, 290, 746
NciI CCSGG 2 cut(s) 369, 370
NdeII GATC 7 cut(s) 43, 118, 181, 277, 392, 414, 434
NlaIII CATG 6 cut(s) 119, 145, 229, 421, 577, 740
NmuCI GTSAC 1 cut(s) 360
NspI RCATGY 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 775
PagI TCATGA 1 cut(s) 417
PciI ACATGT 1 cut(s) 141
PciSI GCTCTTC 1 cut(s) 662
PdmI GAANNNNTTC 1 cut(s) 646
PfeI GAWTC 2 cut(s) 409, 634
PkrI GCNGC 4 cut(s) 401, 728, 751, 762
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PscI ACATGT 1 cut(s) 141
Psp124BI GAGCTC 1 cut(s) 689
PspPI GGNCC 2 cut(s) 607, 733
RsaI GTAC 2 cut(s) 310, 769
RsaNI GTAC 2 cut(s) 309, 768
RseI CAYNNNNRTG 3 cut(s) 285, 741, 775
SacI GAGCTC 1 cut(s) 689
SapI GCTCTTC 1 cut(s) 662
SatI GCNGC 4 cut(s) 400, 727, 750, 761
Sau3AI GATC 7 cut(s) 43, 118, 181, 277, 392, 414, 434
Sau96I GGNCC 2 cut(s) 607, 733
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 369, 370
SduI GDGCHC 2 cut(s) 689, 701
SfaNI GCATC 2 cut(s) 58, 747
SinI GGWCC 2 cut(s) 607, 733
SmaI CCCGGG 1 cut(s) 370
SmiMI CAYNNNNRTG 3 cut(s) 285, 741, 775
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
Sse9I AATT 3 cut(s) 13, 131, 334
SsiI CCGC 5 cut(s) 101, 177, 489, 661, 761
SspI AATATT 1 cut(s) 28
SstI GAGCTC 1 cut(s) 689
StyD4I CCNGG 2 cut(s) 367, 368
TaaI ACNGT 1 cut(s) 767
TaqI TCGA 3 cut(s) 192, 470, 637
TasI AATT 3 cut(s) 13, 131, 334
TatI WGTACW 2 cut(s) 308, 767
TauI GCSGC 1 cut(s) 763
TfiI GAWTC 2 cut(s) 409, 634
TscAI CASTG 3 cut(s) 369, 670, 770
TseFI GTSAC 1 cut(s) 360
TseI GCWGC 3 cut(s) 399, 726, 749
Tsp45I GTSAC 1 cut(s) 360
TspDTI ATGAA 4 cut(s) 144, 214, 303, 327
TspGWI ACGGA 1 cut(s) 502
TspMI CCCGGG 1 cut(s) 368
TspRI CASTG 3 cut(s) 369, 670, 770
VpaK11BI GGWCC 2 cut(s) 607, 733
XapI RAATTY 2 cut(s) 131, 334
XceI RCATGY 1 cut(s) 145
XmaI CCCGGG 1 cut(s) 368
XmnI GAANNNNTTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.