MD13G1253900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015341
Physical Location & Seq
Forward (+)
27406146 .. 27407219
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1253900.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGAGGACGAAAATTACGTTGAGAATATTCCTCGTTATCGAGATCAATCAGAAGAAGAAGAAGATGCAGAAGAAGCACTCTCTCTCAGCGACCTTCCACTAGACAATATCCACGAACAAGTCAACGAAGTCCACGACAGGTCCTCCAAGCACCTGCACACTCGCCGTTCTTCAGCCGATCAGTTTTTCGAGTTCTTCAGCGACTTCAGCTCCGACAGCTTCATGTGTTCAGCCGAAGATATCATCGTCTGCGGGAAACTCATACCCTTCAAAGATCACACAAATGAAGCCCCTAAGACGACATGTACTAACAATGGTTATCACAAAAAAGATTCATCTTTTCGAAGGCGGTCAGAGTCGCTGCCTGGGTTACAAAGTTCGGTAGCACGATCAAGCAGCACAAAGAATCAGATCATGATGAGGAACAGCAGATCCTTGGATTATCAAAAGCTTCACCGGCAATCTTCGATGGTATCCCCGACCCCGGCAGAGATGGAGAGGAATTCTTCCACGAAGAGTGTTGGAAAATACGATAAGAAGAGTGGTAACAAGCCAAAGTGGTTTTTTCTCATGTTTGGGATTGTGAGGTTTCCGTCTGAGATGGACCTAAGCGACATCAAGAACCGGCAGATTCGACGAAACCCATCCACCACGCTCTTCCGGAGTGAAGCCTCTGACAATCGGAGCTCCGAAAAGGGCTCCTGGAGACTGCTCAGGGCATTGAGCTGCAAGGACCATGCAAGTGTTGCTGTAACGCCGCCGTACTGTACACAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

29.77

Weight (kDa)

7.09

Isoelectric Point (pI)

69.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30230
fragaria_vesca FvH4_4g08220
malus_domestica MD13G1253900.v1.1 MD16G1257900.v1.1
prunus_persica Prupe.1G086100_v2.0.a1
pyrus_communis pycom13g22430
rosa_chinensis RchiOBHm_Chr4g0402471
rosa_laevigata RLG00000009081
rosa_multiflora Rmu_co8180070.1_g000001 Rmu_ssc0000330.1_g000029
rosa_roxburghii Rroxscaffold_5G00347160
rosa_rugosa Rorug04G0035700
rosa_samantha Rh4BG105500 Rh4CG119900 Rh4DG104900
rosa_wichuraiana Rw4G009060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 162
AccIII TCCGGA 1 cut(s) 660
AciI CCGC 3 cut(s) 252, 349, 758
AclWI GGATC 1 cut(s) 426
AcsI RAATTY 1 cut(s) 502
AcuI CTGAAG 3 cut(s) 156, 181, 190
AfaI GTAC 3 cut(s) 307, 764, 769
AfiI CCNNNNNNNGG 1 cut(s) 484
AflIII ACRYGT 1 cut(s) 302
AgsI TTSAA 1 cut(s) 271
AjnI CCWGG 2 cut(s) 364, 701
AleI CACNNNNGTG 1 cut(s) 775
AloI GAACNNNNNNTCC 2 cut(s) 416, 448
AluBI AGCT 5 cut(s) 210, 219, 451, 687, 726
AluI AGCT 5 cut(s) 210, 219, 451, 687, 726
Alw21I GWGCWC 1 cut(s) 689
Alw26I GTCTC 1 cut(s) 700
AlwI GGATC 1 cut(s) 426
Aor13HI TCCGGA 1 cut(s) 660
ApeKI GCWGC 3 cut(s) 361, 396, 726
ApoI RAATTY 1 cut(s) 502
AspS9I GGNCC 3 cut(s) 141, 604, 733
AsuC2I CCSGG 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 446
AsuII TTCGAA 1 cut(s) 343
AvaII GGWCC 3 cut(s) 141, 604, 733
BanII GRGCYC 2 cut(s) 689, 701
Bbv12I GWGCWC 1 cut(s) 689
BbvI GCAGC 3 cut(s) 348, 408, 713
BccI CCATC 4 cut(s) 463, 487, 595, 652
BceAI ACGGC 2 cut(s) 150, 745
BciT130I CCWGG 2 cut(s) 366, 703
BciVI GTATCC 1 cut(s) 484
BcnI CCSGG 1 cut(s) 485
BcoDI GTCTC 1 cut(s) 700
BfaI CTAG 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 162
BfuI GTATCC 1 cut(s) 484
BisI GCNGC 4 cut(s) 362, 397, 727, 758
BlsI GCNGC 4 cut(s) 363, 398, 728, 759
Bme1390I CCNGG 3 cut(s) 366, 485, 703
Bme18I GGWCC 3 cut(s) 141, 604, 733
BmgT120I GGNCC 3 cut(s) 141, 604, 733
BmiI GGNNCC 1 cut(s) 700
BmrFI CCNGG 3 cut(s) 366, 485, 703
BmsI GCATC 1 cut(s) 55
BpmI CTGGAG 1 cut(s) 724
Bpu10I CCTNAGC 2 cut(s) 608, 713
Bpu14I TTCGAA 1 cut(s) 343
BpuMI CCSGG 1 cut(s) 485
BsaJI CCNNGG 3 cut(s) 365, 435, 483
BsaWI WCCGGW 1 cut(s) 660
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse118I RCCGGY 2 cut(s) 456, 624
BseAI TCCGGA 1 cut(s) 660
BseBI CCWGG 2 cut(s) 366, 703
BseDI CCNNGG 3 cut(s) 365, 435, 483
BseGI GGATG 1 cut(s) 644
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMII CTCAG 3 cut(s) 100, 588, 727
BseXI GCAGC 3 cut(s) 348, 408, 713
BsgI GTGCAG 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 689
BsiSI CCGG 4 cut(s) 457, 485, 625, 661
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 1 cut(s) 700
Bsp119I TTCGAA 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 689, 701
Bsp13I TCCGGA 1 cut(s) 660
Bsp1407I TGTACA 1 cut(s) 767
Bsp143I GATC 6 cut(s) 43, 178, 274, 389, 411, 431
BspACI CCGC 3 cut(s) 252, 349, 758
BspCNI CTCAG 3 cut(s) 99, 589, 726
BspEI TCCGGA 1 cut(s) 660
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 700
BspMI ACCTGC 1 cut(s) 162
BspPI GGATC 1 cut(s) 426
BspQI GCTCTTC 1 cut(s) 662
BspT104I TTCGAA 1 cut(s) 343
BsrFI RCCGGY 2 cut(s) 456, 624
BsrGI TGTACA 1 cut(s) 767
BssAI RCCGGY 2 cut(s) 456, 624
BssECI CCNNGG 3 cut(s) 365, 435, 483
BssMI GATC 6 cut(s) 43, 178, 274, 389, 411, 431
BssT1I CCWWGG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 366, 703
Bst4CI ACNGT 1 cut(s) 767
Bst6I CTCTTC 3 cut(s) 509, 533, 662
BstAPI GCANNNNNTGC 1 cut(s) 746
BstAUI TGTACA 1 cut(s) 767
BstBI TTCGAA 1 cut(s) 343
BstDEI CTNAG 5 cut(s) 86, 294, 597, 608, 713
BstF5I GGATG 1 cut(s) 644
BstKTI GATC 6 cut(s) 46, 181, 277, 392, 414, 434
BstMAI GTCTC 1 cut(s) 700
BstMBI GATC 6 cut(s) 43, 178, 274, 389, 411, 431
BstMWI GCNNNNNNNGC 5 cut(s) 74, 207, 216, 457, 746
BstNI CCWGG 2 cut(s) 366, 703
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 3 cut(s) 364, 483, 701
BstV1I GCAGC 3 cut(s) 348, 408, 713
BstX2I RGATCY 1 cut(s) 431
BstYI RGATCY 1 cut(s) 431
BsuI GTATCC 1 cut(s) 484
BtsCI GGATG 1 cut(s) 644
BveI ACCTGC 1 cut(s) 162
CciI TCATGA 1 cut(s) 414
Cfr10I RCCGGY 2 cut(s) 456, 624
Cfr13I GGNCC 3 cut(s) 141, 604, 733
Csp6I GTAC 3 cut(s) 306, 763, 768
CviAII CATG 5 cut(s) 223, 303, 415, 571, 737
CviQI GTAC 3 cut(s) 306, 763, 768
DdeI CTNAG 5 cut(s) 86, 294, 597, 608, 713
DpnI GATC 6 cut(s) 45, 180, 276, 391, 413, 433
DpnII GATC 6 cut(s) 43, 178, 274, 389, 411, 431
Eam1104I CTCTTC 3 cut(s) 509, 533, 662
EarI CTCTTC 3 cut(s) 509, 533, 662
Ecl136II GAGCTC 1 cut(s) 687
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 2 cut(s) 689, 701
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 3 cut(s) 141, 604, 733
Eco53kI GAGCTC 1 cut(s) 687
Eco57I CTGAAG 3 cut(s) 156, 181, 190
EcoICRI GAGCTC 1 cut(s) 687
EcoO109I RGGNCCY 1 cut(s) 141
EcoRI GAATTC 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 364, 701
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 2 cut(s) 689, 701
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 5 cut(s) 226, 306, 418, 574, 740
FaiI YATR 6 cut(s) 224, 263, 304, 416, 572, 738
FatI CATG 5 cut(s) 222, 302, 414, 570, 736
FauI CCCGC 1 cut(s) 245
Fnu4HI GCNGC 4 cut(s) 362, 397, 727, 758
FokI GGATG 1 cut(s) 631
FriOI GRGCYC 2 cut(s) 689, 701
Fsp4HI GCNGC 4 cut(s) 362, 397, 727, 758
FspBI CTAG 1 cut(s) 101
GluI GCNGC 4 cut(s) 362, 397, 727, 758
GsuI CTGGAG 1 cut(s) 724
HapII CCGG 4 cut(s) 457, 485, 625, 661
Hin1II CATG 5 cut(s) 226, 306, 418, 574, 740
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 4 cut(s) 332, 356, 406, 631
HpaII CCGG 4 cut(s) 457, 485, 625, 661
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 3 cut(s) 124, 133, 770
Hpy188I TCNGA 8 cut(s) 52, 214, 355, 411, 598, 676, 684, 691
Hpy188III TCNNGA 4 cut(s) 41, 415, 619, 661
Hpy8I GTNNAC 3 cut(s) 124, 133, 770
Hpy99I CGWCG 1 cut(s) 639
HpyAV CCTTC 3 cut(s) 104, 277, 339
HpyCH4III ACNGT 1 cut(s) 767
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 4 cut(s) 68, 157, 729, 740
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 207, 216, 457, 746
HpyF3I CTNAG 5 cut(s) 86, 294, 597, 608, 713
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 5 cut(s) 226, 306, 418, 574, 740
Kpn2I TCCGGA 1 cut(s) 660
Kzo9I GATC 6 cut(s) 43, 178, 274, 389, 411, 431
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 4 cut(s) 215, 684, 692, 704
Lsp1109I GCAGC 3 cut(s) 348, 408, 713
LweI GCATC 1 cut(s) 55
MaeI CTAG 1 cut(s) 101
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 3 cut(s) 369, 545, 751
MalI GATC 6 cut(s) 45, 180, 276, 391, 413, 433
MboI GATC 6 cut(s) 43, 178, 274, 389, 411, 431
MflI RGATCY 1 cut(s) 431
MhlI GDGCHC 2 cut(s) 689, 701
MluCI AATT 2 cut(s) 13, 502
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 2 cut(s) 237, 502
MnlI CCTC 6 cut(s) 42, 154, 414, 492, 579, 682
MroI TCCGGA 1 cut(s) 660
MslI CAYNNNNRTG 3 cut(s) 282, 741, 775
MspI CCGG 4 cut(s) 457, 485, 625, 661
MspR9I CCNGG 3 cut(s) 366, 485, 703
MvaI CCWGG 2 cut(s) 366, 703
MwoI GCNNNNNNNGC 5 cut(s) 74, 207, 216, 457, 746
NciI CCSGG 1 cut(s) 485
NdeII GATC 6 cut(s) 43, 178, 274, 389, 411, 431
NlaIII CATG 5 cut(s) 226, 306, 418, 574, 740
NlaIV GGNNCC 1 cut(s) 700
NspI RCATGY 1 cut(s) 306
NspV TTCGAA 1 cut(s) 343
OliI CACNNNNGTG 1 cut(s) 775
PagI TCATGA 1 cut(s) 414
PaqCI CACCTGC 1 cut(s) 162
PciI ACATGT 1 cut(s) 302
PciSI GCTCTTC 1 cut(s) 662
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 132
PfeI GAWTC 3 cut(s) 332, 406, 631
PfoI TCCNGGA 1 cut(s) 701
PkrI GCNGC 4 cut(s) 363, 398, 728, 759
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 141
PscI ACATGT 1 cut(s) 302
Psp124BI GAGCTC 1 cut(s) 689
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 364, 701
PspGI CCWGG 2 cut(s) 364, 701
PspN4I GGNNCC 1 cut(s) 700
PspPI GGNCC 3 cut(s) 141, 604, 733
PspPPI RGGWCCY 1 cut(s) 141
PsuI RGATCY 1 cut(s) 431
RsaI GTAC 3 cut(s) 307, 764, 769
RsaNI GTAC 3 cut(s) 306, 763, 768
RseI CAYNNNNRTG 3 cut(s) 282, 741, 775
SacI GAGCTC 1 cut(s) 689
SapI GCTCTTC 1 cut(s) 662
SatI GCNGC 4 cut(s) 362, 397, 727, 758
Sau3AI GATC 6 cut(s) 43, 178, 274, 389, 411, 431
Sau96I GGNCC 3 cut(s) 141, 604, 733
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 3 cut(s) 366, 485, 703
SduI GDGCHC 2 cut(s) 689, 701
SfaNI GCATC 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 343
SinI GGWCC 3 cut(s) 141, 604, 733
SmiMI CAYNNNNRTG 3 cut(s) 282, 741, 775
Sse9I AATT 2 cut(s) 13, 502
SsiI CCGC 3 cut(s) 252, 349, 758
SspI AATATT 1 cut(s) 28
SspMI CTAG 1 cut(s) 101
SstI GAGCTC 1 cut(s) 689
StyD4I CCNGG 3 cut(s) 364, 483, 701
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 767
TaiI ACGT 1 cut(s) 21
TaqI TCGA 5 cut(s) 40, 189, 343, 467, 634
TasI AATT 2 cut(s) 13, 502
TatI WGTACW 2 cut(s) 305, 767
TauI GCSGC 1 cut(s) 760
TfiI GAWTC 3 cut(s) 332, 406, 631
TseI GCWGC 3 cut(s) 361, 396, 726
TspDTI ATGAA 3 cut(s) 211, 300, 324
TspGWI ACGGA 1 cut(s) 582
VpaK11BI GGWCC 3 cut(s) 141, 604, 733
XapI RAATTY 1 cut(s) 502
XceI RCATGY 1 cut(s) 306
XspI CTAG 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.