Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0010438

Physical Location & Seq

Genomic Coordinates Forward (+)

14502920 .. 14519594

Gene Structure 16675 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g10800.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3723 bp

ATGGGACACAGTTCGAAGAAGAAGAAGCGCGGCGGAGCAGGAGGGAGTGGGAAGAAGAAGAAGCCCTTGAAAGATCATGGCGAGTCCTACGATAACGAGCTTCTTTCTGAAGAGATCACTGCTCTGTCTGCCATTTTTCAAGATGACTGCAAGATTGTTTCGGGATCACAACCTCAAATCATCATCAAGCTTAGGCCTCACTCAAAGGATATGGGTTATGAGGATCTGGATGTCTCCGCACTTCTTACAGTTAGGTGCTTACCAGGGTATCCTAACAAGTGTCCCAAGTTGCAGATCACTCCAGAGAAAGGATTATCCATAACTGATGCTGATAAGCTACTATCTCTTATCAATGATCAGGCAAGTTCCAATGCCCGAGAAGGGCGAGTAATGATCTTCAATTTGGTGGAGGCAGCACAGGAATTCCTATCTGAGATTGTTCCAGTGGGTCAATCACAGGGACCGGTAACATGTTCAACAGCGGATAGCAGTGCTCAATTGTTTCAAAATGACGCTGCAGTTTCTAGTAGCAAGAAGGGGCCTTTTGTTTATGGTTTCATAGACCTCTTCAGTGGCTCTGGAAAGTCTTGGAGTTGGAGTTTTGAAGTTAACGAGACTAAAGGGATAAATTCTAGTGTCCAGTTGCCTAGATTGGATGGATCCAAACTCATGCATGAGATTCAGGAAAATAAACTTGATAAGGAAGCAGGACCTTTGAAATCACAAGAGATAAAGCAAGCCTCAGTAATTTCCCCTAATGTAAAGTTAGAAACCCTTGAAGAAGAGAGTGAAGACAGCAAGAAGAGTAATTATTCTGTGGATTCAAGTGGATTTCTATTAGAAGAAATGGACGGAAACAGTGAAGAAACTGAGAATGAGAATTCAGTTCCTGAGGAGGATTCAACCGAAGATGATTGGGAATCCAGAAGTCAGCAGTCGGACTCACTCTCTTTGACTTCCTTGGCTCATGATCAAGGATCCCAAAATATTAAAAGGGATCTGATAATGGTTCATCTGCTCCGTCTTGCCTGCACTAAAGGACCATTGGCTGATGCTTTGCCACAGATAACCACAGAACTGCAAAACATAGGGGTCTTATCAGAATGGGCAGGGGATTTAGCTACGAAACCAATTTCACTTCTAAATCGAAAATTTAATCATGCCTTCGGACAACATATGGTTTCATCCCGTATCTCTAAATTCTGGGAGCTCACTTCTGATTTAGAAGAGCCAAGTACATCTCTCCCAAGTTCTCGATATCTCAATGACTTTGAGGAGCTACATTCCCTTGGTCATGGTGGCTTTGGCCATGTTGTATTGTGCAAAAATAAGCTAGATGGAAGGCAGTATGCAGTAAAGAAAATTCGCCTTAAGGATAAAAGCCTGCCACTTAATGACCGGATATTGAGGGAAGTCGCTACACTTTCTCGTTTGCAACATCAACATGTTGTACGGTACTATCAGGCCTGGTTTGAAACAGGAGGTGTTGGTGCTCATGGTGATACTACTTGGGGTTCAAGAACTGCAGCCAGCTCCACTTTCAGCTTTAAAGGTACAAGCTCAGCAGATGATATTGGGAATGAGAATAAACTTGAATCAACATATTTGTACATTCAAATGGAGTACTGTCCTAGGACATTACGCCAGGTTTTTGAGTCCTATAGTCATTTTGACAAAGAATTAGCATGGCATTTGTTTCGTCAAATTGTTGAAGGCCTGGCACACATACATGGACAAGGAATCATCCATCGGGACTTAACCCCGAATAACATATTCTTTGATGCACGTAATGATATTAAAATCGGGGATTTCGGTTTGGCCAAGTTTTTAAAGTTCGAGCAGCTGGATCAAGATCCAATCCCTGCAGATACAACTGGAGTTTCTCTAGATGGTACTGGTCAAGTTGGTACATATTTTTACACAGCACCTGAAATTGAGCAAGGGTGGCCGAAGATTGATGAAAAGGCTGATATGTACAGCTTGGGAATTGTATTTTTGGAGCTATGGCACCCATTTGGGACTGCAATGGAACGGCACCTTGTCCTATCTGACTTAAAACAGAAGGGTGTGCTGCCTTCTGCTTGGGTTGCTGAATATCCGGAACAAGCTTCCTTGTTGCGACTCTTAATGTCCCCAAGTCCGTCAGATCGCCCTTCTGCTACAGAACTTATAAAGCATGCATTTCCACCACGAATGGAATCTGAGTTGCTCGACAATATTCTACGAACAATGCAAACCTCAGAGGACAGAACTGTATATGATAAAGTTCTAAATGCTATATTTGATGAGGAGATGCTAAGCTTGAAAGACCAGCAACATCATGATGGTAGGTTAAGGTTGGCTGGTGGTGATACTTCTGCAATTCAGTATTCAGATTTAGATACCGAGGCTCGTGATTATGTTGTTGAGATCACAAGGGAGGTGTTCAGACAACATTGTGCCAAGCACCTTGAAGTAATACCTATGCGTCTGTTGGATGATTGCCAACATTTTATGAGGAACACTGTCAAACTTTTGACCCATGGAGGAGATATGCTTGAACTTTTGCATGAGCTGCGCTTGCCTTTTGTTAGTTGGGTGATTTCGAATCAGAAATCTTCATTCAAAAGATATGAAATTTCATGTGTCTACAGAAGGCCAATTGGACATGCATCCCCAAGTCGATACCTGCAGGGTGATTTTGACATTATTGGAGGTGCATCAGCGTTGACAGAGGCAGAAGTTATCAAGGTGACAAGGGACATTGTAACCCGTTTTTTTCATTCGGAGTTCTGTGACATTCATCTAAATCACGGGGACTTACTGGAGGCAATTTGGTCTTGGGTAGGAGTCAAGGCAGACCATAGACAAAAAGTAGCTGAGCTTCTTTCTATGATGGGCTCCTTACGTCCTCAGTCATCTGAACGAAAATCCAAATGGGTGGTAATAAGGCGTCAGCTTTTGCAGGAACTTAATTTACAAGAGGCTGTGGTGAATAGATTGCAGACTGTTGGTTTAAGGTTCTGTGGAGCTGCAGATCAGGCACTTCCCAGACTTAGGGGAGCCTTGCCAAATGATAAACCAACACGTACGGCACTTGATGAGTTGTCAGATCTCTGTAACTACTTGAGAGCTTGGAGGATTGAAGGACATGTTTATATAGATCCACTTATTCCCCCAACTGAGAGTTATCATAGAGATTTGTTTTTCCAGGTATATTTGGTGAAGGATAGTAGTCCTGGATCACCTACCGAAGGTGCACTCCTTGCTATTGGGGGTCGCAATGATTATTTGCTTCATCATATGTGGGGTTTTGAACATAAATCTAGTCCTCCTGGTTCTGTTGGGACCAGCCTTGCTTTAGAGACCATAATTCAGCATTATCCGGTGGATTTTAGACCAATTAGAAATGAAACTAGCAGCAGTGTTCTTGTTTGTTCAAAGGGAGGAGGGGGTCTATTGGCAGAACGCATGGAACTAGTCAATGAGCTCTGGGAAGAAAATATTAAGGCTGAGTTTCTCCCAACACCCGATCCAAGTCTTACAGAGCAGTATGAATATGCCAACGAACATGATATTAAATGTCTTGTCATCATAACAGACACTGGTGTTTCTCAGAAAGGTTCTGTTAAGGTCCGTCATCTTGAATTGAAGAAGGAAAAGGAGGTTGAAAGGGAATTTCTCGTCAGGTTTCTGCTGGATGCAGTGGCAATACAATTTAAAAATCCTTCCATTTGGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0014070 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034198 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0046677 GO:0050896 GO:0051716 GO:0060992 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928

KEGG Pathways

Metabolic & Signaling

ko04138 ko04140 ko04141 ko05160 ko05162 ko05164 ko05168 ko05169 map04138 map04140 map04141 map05160 map05162 map05164 map05168 map05169

Pfam Domains

Protein Families

HGTP_anticodon2 Pkinase RWD tRNA-synt_His

Protein Analysis

1241

Amino Acids

139.45

Weight (kDa)

5.6

Isoelectric Point (pI)

43.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	10 - 272	3e-35	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	78 - 269	1.5e-15	Protein tyrosine and serine/threonine kinase
tRNA-synt_His	PF13393	418 - 660	1.9e-08	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	679 - 772	2.7e-09	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	680 - 770	4.1e-08	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59410 AT3G59410 AT3G59410 AT3G59410 AT3G59410
fragaria_vesca	FvH4_4g10800 FvH4_4g10800
malus_domestica	MD04G1040400.v1.1
prunus_persica	Prupe.1G194100_v2.0.a1 Prupe.1G194100_v2.0.a1
pyrus_communis	pycom04g03520
rosa_chinensis	RchiOBHm_Chr4g0406781
rosa_laevigata	RLG00000008759
rosa_multiflora	Rmu_sc0006408.1_g000006
rosa_roxburghii	Rroxscaffold_5G00351540
rosa_rugosa	Rorug04G0072900
rosa_samantha	Rh4AG140400 Rh4BG137400 Rh4CG147300 Rh4DG134400
rosa_wichuraiana	Rw4G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2171
Acc36I	ACCTGC	1 cut(s)	2676
AccB1I	GGYRCC	2 cut(s)	2007, 2034
AccI	GTMKAC	1 cut(s)	2628
AccII	CGCG	1 cut(s)	30
AccIII	TCCGGA	1 cut(s)	2098
AciI	CCGC	4 cut(s)	30, 33, 237, 482
AcoI	YGGCCR	3 cut(s)	1306, 1818, 1946
AcsI	RAATTY	8 cut(s)	422, 628, 880, 1151, 1199, 1362, 2616, 3656
AcuI	CTGAAG	2 cut(s)	129, 553
AcyI	GRCGYC	1 cut(s)	2932
AfiI	CCNNNNNNNGG	4 cut(s)	308, 381, 3036, 3233
AflII	CTTAAG	1 cut(s)	1370
AflIII	ACRYGT	4 cut(s)	470, 1444, 3065, 3130
AgeI	ACCGGT	1 cut(s)	463
AhlI	ACTAGT	1 cut(s)	3457
AjnI	CCWGG	7 cut(s)	262, 1466, 1644, 1716, 3189, 3217, 3313
Alw21I	GWGCWC	5 cut(s)	496, 1212, 1495, 3241, 3471
Alw26I	GTCTC	3 cut(s)	238, 608, 3338
Alw44I	GTGCAC	1 cut(s)	3237
AlwNI	CAGNNNCTG	3 cut(s)	890, 1928, 3584
Ama87I	CYCGRG	1 cut(s)	375
Aor13HI	TCCGGA	1 cut(s)	2098
AoxI	GGCC	8 cut(s)	194, 539, 1306, 1464, 1714, 1818, 1946, 2636
ApaLI	GTGCAC	1 cut(s)	3237
ApeKI	GCWGC	8 cut(s)	413, 515, 1526, 1840, 2071, 2554, 3011, 3399
ApoI	RAATTY	8 cut(s)	422, 628, 880, 1151, 1199, 1362, 2616, 3656
AsiGI	ACCGGT	1 cut(s)	463
AspA2I	CCTAGG	1 cut(s)	1631
AspLEI	GCGC	2 cut(s)	30, 2559
AspS9I	GGNCC	6 cut(s)	461, 539, 710, 1040, 3327, 3613
AsuHPI	GGTGA	8 cut(s)	1511, 2360, 2590, 2687, 2743, 2983, 3214, 3216
AsuII	TTCGAA	2 cut(s)	14, 2585
AvaI	CYCGRG	1 cut(s)	375
AvaII	GGWCC	5 cut(s)	461, 710, 1040, 3327, 3613
AvrII	CCTAGG	1 cut(s)	1631
AxyI	CCTNAGG	1 cut(s)	891
BaeGI	GKGCMC	1 cut(s)	3241
BaeI	ACNNNNGTAYC	2 cut(s)	251, 284
BalI	TGGCCA	2 cut(s)	1308, 1820
BamHI	GGATCC	2 cut(s)	659, 977
BanI	GGYRCC	2 cut(s)	2007, 2034
BanII	GRGCYC	3 cut(s)	1212, 2882, 3471
BarI	GAAGNNNNNNTAC	2 cut(s)	2847, 2879
BauI	CACGAG	1 cut(s)	2391
BbsI	GAAGAC	1 cut(s)	798
Bbv12I	GWGCWC	5 cut(s)	496, 1212, 1495, 3241, 3471
BbvI	GCAGC	8 cut(s)	425, 502, 1538, 1852, 2058, 2541, 2998, 3411
BccI	CCATC	6 cut(s)	650, 1331, 1755, 1883, 2318, 2869
BceAI	ACGGC	2 cut(s)	2048, 3087
BcgI	CGANNNNNNTGC	2 cut(s)	1679, 1713
BciT130I	CCWGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
BciVI	GTATCC	1 cut(s)	279
BclI	TGATCA	2 cut(s)	355, 970
BcoDI	GTCTC	3 cut(s)	238, 608, 3338
BcuI	ACTAGT	1 cut(s)	3457
BfaI	CTAG	9 cut(s)	525, 633, 648, 1334, 1632, 1886, 3306, 3396, 3458
BfmI	CTRYAG	8 cut(s)	516, 1524, 1660, 1863, 2160, 2629, 2669, 3012
BfrI	CTTAAG	1 cut(s)	1370
BfuAI	ACCTGC	1 cut(s)	2676
BfuI	GTATCC	1 cut(s)	279
BglII	AGATCT	1 cut(s)	3091
BisI	GCNGC	9 cut(s)	31, 414, 516, 1527, 1841, 2072, 2555, 3012, 3400
BlnI	CCTAGG	1 cut(s)	1631
BlpI	GCTNAGC	3 cut(s)	1561, 2297, 2859
BlsI	GCNGC	9 cut(s)	32, 415, 517, 1528, 1842, 2073, 2556, 3013, 3401
BmcAI	AGTACT	1 cut(s)	1625
Bme1390I	CCNGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
Bme18I	GGWCC	5 cut(s)	461, 710, 1040, 3327, 3613
BmeT110I	CYCGRG	1 cut(s)	375
BmgT120I	GGNCC	6 cut(s)	461, 539, 710, 1040, 3327, 3613
BmiI	GGNNCC	9 cut(s)	462, 540, 661, 979, 2009, 2036, 2881, 3043, 3328
BmrFI	CCNGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
BmsI	GCATC	7 cut(s)	316, 1042, 1771, 2283, 2660, 2708, 3670
BpiI	GAAGAC	1 cut(s)	798
BpmI	CTGGAG	3 cut(s)	285, 1896, 2825
Bpu10I	CCTNAGC	1 cut(s)	191
Bpu1102I	GCTNAGC	3 cut(s)	1561, 2297, 2859
Bpu14I	TTCGAA	2 cut(s)	14, 2585
BpuEI	CTTGAG	1 cut(s)	3127
BsaAI	YACGTR	2 cut(s)	1787, 3068
BsaBI	GATNNNNATC	1 cut(s)	1851
BsaHI	GRCGYC	1 cut(s)	2932
BsaI	GGTCTC	1 cut(s)	3338
BsaJI	CCNNGG	6 cut(s)	263, 960, 1288, 1631, 2385, 2521
BsaWI	WCCGGW	4 cut(s)	463, 1398, 2098, 3364
BsaXI	ACNNNNNCTCC	2 cut(s)	583, 613
Bsc4I	CCNNNNNNNGG	4 cut(s)	308, 381, 3036, 3233
Bse118I	RCCGGY	1 cut(s)	463
Bse1I	ACTGG	6 cut(s)	443, 640, 1879, 1900, 2808, 3589
Bse21I	CCTNAGG	1 cut(s)	891
Bse3DI	GCAATG	2 cut(s)	2031, 3268
Bse8I	GATNNNNATC	1 cut(s)	1851
BseAI	TCCGGA	1 cut(s)	2098
BseBI	CCWGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
BseDI	CCNNGG	6 cut(s)	263, 960, 1288, 1631, 2385, 2521
BseGI	GGATG	7 cut(s)	235, 661, 1184, 1743, 2482, 2651, 3685
BseJI	GATNNNNATC	1 cut(s)	1851
BseLI	CCNNNNNNNGG	4 cut(s)	308, 381, 3036, 3233
BseMI	GCAATG	2 cut(s)	2031, 3268
BseNI	ACTGG	6 cut(s)	443, 640, 1879, 1900, 2808, 3589
BseRI	GAGGAG	5 cut(s)	908, 1289, 2303, 2541, 3441
BseSI	GKGCMC	1 cut(s)	3241
BseXI	GCAGC	8 cut(s)	425, 502, 1538, 1852, 2058, 2541, 2998, 3411
BsgI	GTGCAG	1 cut(s)	1015
Bsh1236I	CGCG	1 cut(s)	30
BshFI	GGCC	8 cut(s)	196, 541, 1308, 1466, 1716, 1820, 1948, 2638
BshNI	GGYRCC	2 cut(s)	2007, 2034
BshTI	ACCGGT	1 cut(s)	463
BsiHKAI	GWGCWC	5 cut(s)	496, 1212, 1495, 3241, 3471
BsiHKCI	CYCGRG	1 cut(s)	375
BsiSI	CCGG	4 cut(s)	464, 1399, 2099, 3365
BsiWI	CGTACG	1 cut(s)	3068
BslFI	GGGAC	9 cut(s)	18, 267, 474, 1766, 2032, 2116, 2753, 2810, 3340
BslI	CCNNNNNNNGG	4 cut(s)	308, 381, 3036, 3233
BsmAI	GTCTC	3 cut(s)	238, 608, 3338
BsmFI	GGGAC	9 cut(s)	18, 267, 474, 1766, 2032, 2116, 2753, 2810, 3340
BsnI	GGCC	8 cut(s)	196, 541, 1308, 1466, 1716, 1820, 1948, 2638
Bso31I	GGTCTC	1 cut(s)	3338
BsoBI	CYCGRG	1 cut(s)	375
Bsp119I	TTCGAA	2 cut(s)	14, 2585
Bsp1286I	GDGCHC	6 cut(s)	496, 1212, 1495, 2882, 3241, 3471
Bsp13I	TCCGGA	1 cut(s)	2098
Bsp1407I	TGTACA	2 cut(s)	1608, 1974
Bsp1720I	GCTNAGC	3 cut(s)	1561, 2297, 2859
Bsp19I	CCATGG	1 cut(s)	2521
BspACI	CCGC	4 cut(s)	30, 33, 237, 482
BspANI	GGCC	8 cut(s)	196, 541, 1308, 1466, 1716, 1820, 1948, 2638
BspEI	TCCGGA	1 cut(s)	2098
BspFNI	CGCG	1 cut(s)	30
BspHI	TCATGA	2 cut(s)	967, 2320
BspLI	GGNNCC	9 cut(s)	462, 540, 661, 979, 2009, 2036, 2881, 3043, 3328
BspMAI	CTGCAG	5 cut(s)	520, 1528, 1867, 2673, 3016
BspMI	ACCTGC	1 cut(s)	2676
BspQI	GCTCTTC	1 cut(s)	1221
BspT104I	TTCGAA	2 cut(s)	14, 2585
BspT107I	GGYRCC	2 cut(s)	2007, 2034
BspTI	CTTAAG	1 cut(s)	1370
BspTNI	GGTCTC	1 cut(s)	3338
BsrDI	GCAATG	2 cut(s)	2031, 3268
BsrFI	RCCGGY	1 cut(s)	463
BsrGI	TGTACA	2 cut(s)	1608, 1974
BsrI	ACTGG	6 cut(s)	443, 640, 1879, 1900, 2808, 3589
BssAI	RCCGGY	1 cut(s)	463
BssECI	CCNNGG	6 cut(s)	263, 960, 1288, 1631, 2385, 2521
BssNI	GRCGYC	1 cut(s)	2932
BssSI	CACGAG	1 cut(s)	2391
BssT1I	CCWWGG	4 cut(s)	960, 1288, 1631, 2521
Bst2BI	CACGAG	1 cut(s)	2391
Bst2UI	CCWGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
Bst4CI	ACNGT	8 cut(s)	11, 250, 860, 1455, 1628, 2254, 2506, 2989
Bst6I	CTCTTC	5 cut(s)	105, 572, 777, 797, 1221
BstACI	GRCGYC	1 cut(s)	2932
BstAFI	CTTAAG	1 cut(s)	1370
BstAPI	GCANNNNNTGC	2 cut(s)	2554, 3245
BstAUI	TGTACA	2 cut(s)	1608, 1974
BstBAI	YACGTR	2 cut(s)	1787, 3068
BstBI	TTCGAA	2 cut(s)	14, 2585
BstC8I	GCNNGC	6 cut(s)	738, 1030, 1385, 1531, 2178, 2561
BstDSI	CCRYGG	1 cut(s)	2521
BstENI	CCTNNNNNAGG	1 cut(s)	3231
BstF5I	GGATG	7 cut(s)	235, 661, 1184, 1743, 2482, 2651, 3685
BstFNI	CGCG	1 cut(s)	30
BstHHI	GCGC	2 cut(s)	30, 2559
BstMAI	GTCTC	3 cut(s)	238, 608, 3338
BstMWI	GCNNNNNNNGC	7 cut(s)	128, 1945, 2087, 2554, 2560, 3020, 3245
BstNI	CCWGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
BstNSI	RCATGY	5 cut(s)	474, 1448, 2180, 2651, 3134
BstSCI	CCNGG	7 cut(s)	262, 1466, 1644, 1716, 3189, 3217, 3313
BstSFI	CTRYAG	8 cut(s)	516, 1524, 1660, 1863, 2160, 2629, 2669, 3012
BstSLI	GKGCMC	1 cut(s)	3241
BstUI	CGCG	1 cut(s)	30
BstV1I	GCAGC	8 cut(s)	425, 502, 1538, 1852, 2058, 2541, 2998, 3411
BstV2I	GAAGAC	1 cut(s)	798
BstX2I	RGATCY	7 cut(s)	223, 659, 977, 997, 1852, 3091, 3142
BstXI	CCANNNNNNTGG	1 cut(s)	2920
BstYI	RGATCY	7 cut(s)	223, 659, 977, 997, 1852, 3091, 3142
Bsu36I	CCTNAGG	1 cut(s)	891
BsuI	GTATCC	1 cut(s)	279
BsuRI	GGCC	8 cut(s)	196, 541, 1308, 1466, 1716, 1820, 1948, 2638
BtgI	CCRYGG	1 cut(s)	2521
BtsCI	GGATG	7 cut(s)	235, 661, 1184, 1743, 2482, 2651, 3685
BtsI	GCAGTG	4 cut(s)	117, 496, 3409, 3690
BtsIMutI	CAGTG	9 cut(s)	117, 450, 496, 577, 865, 2502, 3409, 3582, 3690
BveI	ACCTGC	1 cut(s)	2676
Cac8I	GCNNGC	6 cut(s)	738, 1030, 1385, 1531, 2178, 2561
CaiI	CAGNNNCTG	3 cut(s)	890, 1928, 3584
CciI	TCATGA	2 cut(s)	967, 2320
CfoI	GCGC	2 cut(s)	30, 2559
Cfr10I	RCCGGY	1 cut(s)	463
Cfr13I	GGNCC	6 cut(s)	461, 539, 710, 1040, 3327, 3613
CseI	GACGC	3 cut(s)	521, 2456, 2921
CspAI	ACCGGT	1 cut(s)	463
DraI	TTTAAA	3 cut(s)	1549, 1830, 3700
EaeI	YGGCCR	3 cut(s)	1306, 1818, 1946
Eam1104I	CTCTTC	5 cut(s)	105, 572, 777, 797, 1221
EarI	CTCTTC	5 cut(s)	105, 572, 777, 797, 1221
EciI	GGCGGA	1 cut(s)	48
Ecl136II	GAGCTC	2 cut(s)	1210, 3469
Eco130I	CCWWGG	4 cut(s)	960, 1288, 1631, 2521
Eco147I	AGGCCT	3 cut(s)	196, 1466, 1716
Eco24I	GRGCYC	3 cut(s)	1212, 2882, 3471
Eco31I	GGTCTC	1 cut(s)	3338
Eco32I	GATATC	1 cut(s)	1259
Eco47I	GGWCC	5 cut(s)	461, 710, 1040, 3327, 3613
Eco53kI	GAGCTC	2 cut(s)	1210, 3469
Eco57I	CTGAAG	2 cut(s)	129, 553
Eco81I	CCTNAGG	1 cut(s)	891
Eco88I	CYCGRG	1 cut(s)	375
EcoICRI	GAGCTC	2 cut(s)	1210, 3469
EcoNI	CCTNNNNNAGG	1 cut(s)	3231
EcoO109I	RGGNCCY	2 cut(s)	539, 710
EcoRI	GAATTC	2 cut(s)	422, 880
EcoRII	CCWGG	7 cut(s)	262, 1466, 1644, 1716, 3189, 3217, 3313
EcoRV	GATATC	1 cut(s)	1259
EcoT14I	CCWWGG	4 cut(s)	960, 1288, 1631, 2521
EcoT22I	ATGCAT	3 cut(s)	675, 2182, 2653
EcoT38I	GRGCYC	3 cut(s)	1212, 2882, 3471
ErhI	CCWWGG	4 cut(s)	960, 1288, 1631, 2521
FalI	AAGNNNNNCTT	2 cut(s)	50, 82
FaqI	GGGAC	9 cut(s)	18, 267, 474, 1766, 2032, 2116, 2753, 2810, 3340
FauNDI	CATATG	2 cut(s)	1176, 3282
FbaI	TGATCA	2 cut(s)	355, 970
FblI	GTMKAC	1 cut(s)	2628
Fnu4HI	GCNGC	9 cut(s)	31, 414, 516, 1527, 1841, 2072, 2555, 3012, 3400
FokI	GGATG	7 cut(s)	242, 668, 1171, 1730, 2489, 2638, 3692
FriOI	GRGCYC	3 cut(s)	1212, 2882, 3471
Fsp4HI	GCNGC	9 cut(s)	31, 414, 516, 1527, 1841, 2072, 2555, 3012, 3400
FspBI	CTAG	9 cut(s)	525, 633, 648, 1334, 1632, 1886, 3306, 3396, 3458
GlaI	GCGC	2 cut(s)	29, 2558
GluI	GCNGC	9 cut(s)	31, 414, 516, 1527, 1841, 2072, 2555, 3012, 3400
GsuI	CTGGAG	3 cut(s)	285, 1896, 2825
HaeIII	GGCC	8 cut(s)	196, 541, 1308, 1466, 1716, 1820, 1948, 2638
HapII	CCGG	4 cut(s)	464, 1399, 2099, 3365
HgaI	GACGC	3 cut(s)	521, 2456, 2921
HhaI	GCGC	2 cut(s)	30, 2559
Hin1I	GRCGYC	1 cut(s)	2932
Hin6I	GCGC	2 cut(s)	28, 2557
HinP1I	GCGC	2 cut(s)	28, 2557
HincII	GTYRAC	2 cut(s)	610, 2709
HindII	GTYRAC	2 cut(s)	610, 2709
HindIII	AAGCTT	3 cut(s)	188, 2106, 2299
HpaI	GTTAAC	1 cut(s)	610
HpaII	CCGG	4 cut(s)	464, 1399, 2099, 3365
HphI	GGTGA	8 cut(s)	1511, 2360, 2590, 2687, 2743, 2983, 3214, 3216
Hpy166II	GTNNAC	4 cut(s)	610, 2629, 2709, 3239
Hpy8I	GTNNAC	4 cut(s)	610, 2629, 2709, 3239
HpyCH4III	ACNGT	8 cut(s)	11, 250, 860, 1455, 1628, 2254, 2506, 2989
HpyCH4IV	ACGT	3 cut(s)	1786, 2887, 3067
HpyF10VI	GCNNNNNNNGC	7 cut(s)	128, 1945, 2087, 2554, 2560, 3020, 3245
HpySE526I	ACGT	3 cut(s)	1786, 2887, 3067
Hsp92I	GRCGYC	1 cut(s)	2932
HspAI	GCGC	2 cut(s)	28, 2557
Kpn2I	TCCGGA	1 cut(s)	2098
Ksp22I	TGATCA	2 cut(s)	355, 970
KspAI	GTTAAC	1 cut(s)	610
LguI	GCTCTTC	1 cut(s)	1221
LmnI	GCTCC	9 cut(s)	35, 1023, 1207, 1276, 1538, 1999, 2885, 3008, 3041
Lsp1109I	GCAGC	8 cut(s)	425, 502, 1538, 1852, 2058, 2541, 2998, 3411
LweI	GCATC	7 cut(s)	316, 1042, 1771, 2283, 2660, 2708, 3670
MaeI	CTAG	9 cut(s)	525, 633, 648, 1334, 1632, 1886, 3306, 3396, 3458
MaeII	ACGT	3 cut(s)	1786, 2887, 3067
MaeIII	GTNAC	5 cut(s)	466, 2731, 2746, 2774, 3098
MfeI	CAATTG	2 cut(s)	497, 2640
MflI	RGATCY	7 cut(s)	223, 659, 977, 997, 1852, 3091, 3142
MhlI	GDGCHC	6 cut(s)	496, 1212, 1495, 2882, 3241, 3471
MlsI	TGGCCA	2 cut(s)	1308, 1820
MluNI	TGGCCA	2 cut(s)	1308, 1820
MlyI	GAGTC	5 cut(s)	92, 935, 1664, 2115, 2838
MmeI	TCCRAC	3 cut(s)	575, 918, 2454
Mox20I	TGGCCA	2 cut(s)	1308, 1820
Mph1103I	ATGCAT	3 cut(s)	675, 2182, 2653
MroI	TCCGGA	1 cut(s)	2098
MscI	TGGCCA	2 cut(s)	1308, 1820
MslI	CAYNNNNRTG	4 cut(s)	1443, 1616, 1728, 2322
Msp20I	TGGCCA	2 cut(s)	1308, 1820
MspA1I	CMGCKG	2 cut(s)	482, 1843
MspCI	CTTAAG	1 cut(s)	1370
MspI	CCGG	4 cut(s)	464, 1399, 2099, 3365
MspR9I	CCNGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
MunI	CAATTG	2 cut(s)	497, 2640
MvaI	CCWGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
MvnI	CGCG	1 cut(s)	30
MwoI	GCNNNNNNNGC	7 cut(s)	128, 1945, 2087, 2554, 2560, 3020, 3245
NcoI	CCATGG	1 cut(s)	2521
NdeI	CATATG	2 cut(s)	1176, 3282
NlaIV	GGNNCC	9 cut(s)	462, 540, 661, 979, 2009, 2036, 2881, 3043, 3328
NmuCI	GTSAC	2 cut(s)	2731, 2774
NsiI	ATGCAT	3 cut(s)	675, 2182, 2653
NspI	RCATGY	5 cut(s)	474, 1448, 2180, 2651, 3134
NspV	TTCGAA	2 cut(s)	14, 2585
PaeI	GCATGC	1 cut(s)	2180
PagI	TCATGA	2 cut(s)	967, 2320
PceI	AGGCCT	3 cut(s)	196, 1466, 1716
PciI	ACATGT	3 cut(s)	470, 1444, 3130
PciSI	GCTCTTC	1 cut(s)	1221
PfeI	GAWTC	8 cut(s)	679, 821, 899, 920, 1595, 1740, 2198, 2587
Pfl23II	CGTACG	1 cut(s)	3068
PfoI	TCCNGGA	1 cut(s)	3217
PinAI	ACCGGT	1 cut(s)	463
PkrI	GCNGC	9 cut(s)	32, 415, 517, 1528, 1842, 2073, 2556, 3013, 3401
PleI	GAGTC	5 cut(s)	91, 935, 1663, 2115, 2837
PpsI	GAGTC	5 cut(s)	91, 935, 1663, 2115, 2837
Ppu21I	YACGTR	2 cut(s)	1787, 3068
PpuMI	RGGWCCY	1 cut(s)	710
PscI	ACATGT	3 cut(s)	470, 1444, 3130
PsiI	TTATAA	1 cut(s)	2171
Psp124BI	GAGCTC	2 cut(s)	1212, 3471
Psp5II	RGGWCCY	1 cut(s)	710
Psp6I	CCWGG	7 cut(s)	262, 1466, 1644, 1716, 3189, 3217, 3313
PspGI	CCWGG	7 cut(s)	262, 1466, 1644, 1716, 3189, 3217, 3313
PspLI	CGTACG	1 cut(s)	3068
PspN4I	GGNNCC	9 cut(s)	462, 540, 661, 979, 2009, 2036, 2881, 3043, 3328
PspPI	GGNCC	6 cut(s)	461, 539, 710, 1040, 3327, 3613
PspPPI	RGGWCCY	1 cut(s)	710
PsrI	GAACNNNNNNTAC	2 cut(s)	2940, 2972
PstI	CTGCAG	5 cut(s)	520, 1528, 1867, 2673, 3016
PstNI	CAGNNNCTG	3 cut(s)	890, 1928, 3584
PsuI	RGATCY	7 cut(s)	223, 659, 977, 997, 1852, 3091, 3142
PvuII	CAGCTG	1 cut(s)	1843
RseI	CAYNNNNRTG	4 cut(s)	1443, 1616, 1728, 2322
SacI	GAGCTC	2 cut(s)	1212, 3471
SapI	GCTCTTC	1 cut(s)	1221
SatI	GCNGC	9 cut(s)	31, 414, 516, 1527, 1841, 2072, 2555, 3012, 3400
Sau96I	GGNCC	6 cut(s)	461, 539, 710, 1040, 3327, 3613
SbfI	CCTGCAGG	1 cut(s)	2673
ScaI	AGTACT	1 cut(s)	1625
SchI	GAGTC	5 cut(s)	92, 935, 1664, 2115, 2838
ScrFI	CCNGG	7 cut(s)	264, 1468, 1646, 1718, 3191, 3219, 3315
SdaI	CCTGCAGG	1 cut(s)	2673
SduI	GDGCHC	6 cut(s)	496, 1212, 1495, 2882, 3241, 3471
SfaNI	GCATC	7 cut(s)	316, 1042, 1771, 2283, 2660, 2708, 3670
SfcI	CTRYAG	8 cut(s)	516, 1524, 1660, 1863, 2160, 2629, 2669, 3012
SfuI	TTCGAA	2 cut(s)	14, 2585
SinI	GGWCC	5 cut(s)	461, 710, 1040, 3327, 3613
SmiMI	CAYNNNNRTG	4 cut(s)	1443, 1616, 1728, 2322
SmlI	CTYRAG	2 cut(s)	1370, 3106
SmoI	CTYRAG	2 cut(s)	1370, 3106
SpeI	ACTAGT	1 cut(s)	3457
SphI	GCATGC	1 cut(s)	2180
Sse8387I	CCTGCAGG	1 cut(s)	2673
SseBI	AGGCCT	3 cut(s)	196, 1466, 1716
SsiI	CCGC	4 cut(s)	30, 33, 237, 482
SspI	AATATT	3 cut(s)	988, 2218, 3484
SspMI	CTAG	9 cut(s)	525, 633, 648, 1334, 1632, 1886, 3306, 3396, 3458
SstI	GAGCTC	2 cut(s)	1212, 3471
StuI	AGGCCT	3 cut(s)	196, 1466, 1716
StyD4I	CCNGG	7 cut(s)	262, 1466, 1644, 1716, 3189, 3217, 3313
StyI	CCWWGG	4 cut(s)	960, 1288, 1631, 2521
TaaI	ACNGT	8 cut(s)	11, 250, 860, 1455, 1628, 2254, 2506, 2989
TaiI	ACGT	3 cut(s)	1789, 2890, 3070
TaqI	TCGA	7 cut(s)	14, 1147, 1255, 1836, 2211, 2585, 2662
TatI	WGTACW	4 cut(s)	1235, 1608, 1623, 1974
TauI	GCSGC	1 cut(s)	33
TfiI	GAWTC	8 cut(s)	679, 821, 899, 920, 1595, 1740, 2198, 2587
TscAI	CASTG	9 cut(s)	124, 450, 496, 577, 865, 2509, 3409, 3589, 3690
TseFI	GTSAC	2 cut(s)	2731, 2774
TseI	GCWGC	8 cut(s)	413, 515, 1526, 1840, 2071, 2554, 3011, 3399
Tsp45I	GTSAC	2 cut(s)	2731, 2774
TspGWI	ACGGA	4 cut(s)	867, 1010, 2130, 3605
TspRI	CASTG	9 cut(s)	124, 450, 496, 577, 865, 2509, 3409, 3589, 3690
Vha464I	CTTAAG	1 cut(s)	1370
VneI	GTGCAC	1 cut(s)	3237
VpaK11BI	GGWCC	5 cut(s)	461, 710, 1040, 3327, 3613
XagI	CCTNNNNNAGG	1 cut(s)	3231
XapI	RAATTY	8 cut(s)	422, 628, 880, 1151, 1199, 1362, 2616, 3656
XbaI	TCTAGA	1 cut(s)	1885
XceI	RCATGY	5 cut(s)	474, 1448, 2180, 2651, 3134
XmaJI	CCTAGG	1 cut(s)	1631
XmiI	GTMKAC	1 cut(s)	2628
XspI	CTAG	9 cut(s)	525, 633, 648, 1334, 1632, 1886, 3306, 3396, 3458
ZrmI	AGTACT	1 cut(s)	1625
Zsp2I	ATGCAT	3 cut(s)	675, 2182, 2653

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.