Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0010438

Physical Location & Seq

Genomic Coordinates Reverse (-)

32399709 .. 32417513

Gene Structure 17805 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG147300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3714 bp

ATGGGACATAGTTCGAAAAAGAAGAAGCGCGGAGGAGGAGGAGGAGGCAGTGGGAAGAAGAAGAAGCCCTTGAAAGATCACGCCGGGGCTTACGATAATGACCTTCTTTCTGAAGAGATCACTGCTTTGTGTGCAATATTTCAAGATGACTGCAAAATTGCGTCCGGAGCACAACCTCAAATCACCATCAAGCTTAGGCCTCACTCAAAGGATATGGGTTATGAGGATCTGGATGTCTCTGCACTTCTTTTAGTTAGGTGCTTACCAGGGTATCCTTACAAGTGTCCCAAGTTGCAGATAACTCCAGAGAAAGGATTATCGAAAAGTGACTCTGATAAGCTATTATCTCTTATCAATGATCAGGCAAATTCCAATGCCCGAGAAGGACGGGTAATGATCTTCAATTTGGTCGAGGCAGCTCAGGAATTTCTATCTGAAATTGTGCCAGTGGGTCAATCACAGGGACCAGTAATATGTTCAACTATGGATAACAGTGCTCAGTTGTTTCAAAATGACACTGCAATTTCTAGTAACAAGAAGGGGCCTTTTGTTTATGGTTTCATAGACCTCTTCAGTGGCTCTGGAAAGTCTTGGAGTTGGAGTTTTGGAGTTGACGAGACTAAAGGGATAAATTCTACTGTCCCATTGCCTAGGGTGGATGGGTCCAAACTCATGCATGAGATTCAGGAAAAGAAACATGATAAGCAAGCAGAACCTTTAAAATCACAAGATATAAAGCAAGGGCCAATACTTTCCCCTAATGTCAAGTTAGAAACGCTTGAAGAAGAGAGTGAAGGCAGGGATTCAAGTAGATTTTTGTTGGAAGAAATGGACAGAAATGGTGGTGAAACTGAGAAAGAGAGTTCAGTTCCTGAGGAGGATTCAATAGAAGATGATTGGGAATTCGGAAGTCAGCAGTCGGACTCGCTCTCTTTTACTTCCTTGGCTCATGAGCAAGGATCCCAAAATGTTAAAAGGGATCTAATAATGGTTCATCTGCTCCGTCTCGCCTGCACTAAAGGACCATTGGCTGATGCTTTGCCACAGATAACCACAGAACTGCAAAACATAGGGGTCTTATCAGAATGGGCAAGGGATTTAGCTTCAAAACCAATTTCACTTTTAAATCGAACATTTAATCATGCCTTCGGACAACAGATGGTTTCATCCCGTATCTCTCAATTCTGGGAGCTCACTTCTGATTTAGAAGGACCAAGTACATCTCTCCCAAGTTCTCGATATCTCAATGACTTTGAGGAGCTACAGTCTCTTGGTCATGGTGGCTTTGGCCATGTTGTGTTGTGCAAAAATAAGCTAGATGGAAGGCAGTATGCAGTAAAGAAAATCCGCCTTAAGGATAAAAGCCTGCCACTTGATGACCGCATATTGAGGGAAGTCGCTACACTTTCTCGTTTGCAACATCAACACGTTGTACGATACTATCAGGCCTGGTTTGAAACAGGAGGTGTTGGTGCTCATGGTGATACTACCTGGGGTTCAAGGACTGCAGCCAGCTCCATTTTTAGCTTTAAAGGTACAAGCTCAGCAGATGATCTTGGGAATGAGAATAAACTTGAATCAACATATTTGTACATTCAAATGGAGTATTGCCCTAGGACATTACGCCAGGTTTTTGAGTCATATAGTCAATTTGACAAAGAATTAGCATGGCATTTGTTTCGTCAAATTGTTGAAGGCCTGGCACACATACATGGACAAGGAATCATCCATCGGGACTTAACGCCAAATAACATATTCTTTGATGCACGTAATGATATTAAAATTGGAGATTTTGGCTTGGCCAAGTTTTTGAAGTTGGAGCAGCTGGATCAAGATCCAAGTTTTCCTGCAGATACAACTGGAGTTTCTCTCGATGGTACTGGTCAAGTTGGTACATATTTTTACACCGCACCTGAAATTGAGCAAGGCTGGCCAAAGATTGATGAAAAGGCTGATATGTACAGCTTGGGAATTGTATTTTTCGAGCTATGGCACCCATTTGGGACTGCAATGGAACGGCACCTTGTCCTATCCGACTTGAAACAGAAGGGTGAACTGCCTTCTGCTTGGGTTGCTGAATTTCCAGAACAAGCATCCTTGTTGCGGAGCTTAATGTCCCCAAGTCCATCAGATCGCCCTTCTGCTACAGAACTTATAAAGCATGCATTTCCACCACGAATGGAATCTGAGTTGCTAGACAATATTCTACGAACAATGCAAACCTCTGAGGACAGAAGTGTGTATGATAAAGTTCTGAATGCTATATTTGATGAAGAGATGCTAAGCTTGAAAGACCAGCAACATCATGATGGTAGGTTGAGGTTGGCTGGTAGTGATACTTCTGCGATTCAGTATGCAGATTTAGATTCGGAGGCTCGTGATTATGTTGTTGAGATCACAAGGGAGGTGTTCAGACAACATTGTGCGAAGCACCTTGAAGTAATACCCATGCGTCTGTTGGATGATTGCCAACATTTTATGAGAAGGAACACTGTCAAACTTTTGACCCATGGAGGAGATATGCTTGAACTTTTGCATGAGCTGCGTTTGCCTTTTGTTAGTTGGGTGATTTCGAATCAGAAATCTTCATTCAAAAGATATGAAATTTCATGTGTCTACAGAAGACCAATTGGACATGCACCCCCAAGTCGCTACCTGCAGGGTGATTTCGACATTATTGGAGGTGCATCAGCATTGACAGAGGCAGAAGTGATCAAGGTGACAAGGGACATTGTAACCCGTTTTGTCAATTGGGAGTTCTGCGACATTCATCTAAACCACGGGGACTTACTGGAGGCAATTTGGTCTTGGGTAGGAGTCAAGGCAGACCATAGACAAAAAGTAGCGGAGCTTCTTTCTATGATGGGCTCCTTACGTCCTCAGTCTTCTGAACGGAAATCCAAATGGGTGGTAATAAGGCGTCAGCTTTTGCAGGAACTTAATTTACAAGAGGCTGTTGTGAATAGGTTGCAGACTGTTGGTTTAAGGTTCTGTGGAGCTGCAGATCAGTCACTTCATAGACTTAGGGGAGCCCTGCCAAACGATAAACCAACACGGAAGGCACTTGATGAGTTGTCAGATCTCTGTAGCTACTTGAGAGCTTGGAGGATTGAAGAACATGTTTATATAGATCCACTTATCCCCCCAACTGAGAGTTATCATAGAGACTTGTTTTTCCAGGTATATTTGGTGAAGGATAGTAATCCTGGATCAGTTACCGAAGGTGCACTACTTGCAATTGGGGGTCGGTATGACTATTTGCTTCATCATATGTGGGGTCTTGAACATAAATCTAATCCTCCTGGTTCTGTTGGGACCAGCCTTGCTTTAGAGACCATAATTCAGCATTCTCCTGTGGATTTTAAACCAATTAGCAGAAATGAAGTTAGCAATAGTGTTCTTGTTTGTTCAAAAGGAGGAGGGGGTCTATTGGCAGAACGCATGGAACTAGTCAATGAGCTTTGGGAAGAAAACATTAAGGCTGAGTTTCTCCCAACACCAGATCCAAGCCTTACGGAGCAGTATGAATATGCCAACGAACATGATATTAAATGTCTTGTCATCATAACAGACACTGGTGTTTCTCAGAAAGGTTCTGTTAAGGTCCGTCATCTTGAACTGAAGAAGGAAAAGGAGGTTGAAAGGGAAATTCTCGTCAGGTTTCTGCTGGATGCAATGGCAATACAATTTAAAAATCCTTCTATTTGGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0014070 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034198 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0046677 GO:0050896 GO:0051716 GO:0060992 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928

KEGG Pathways

Metabolic & Signaling

ko04138 ko04140 ko04141 ko05160 ko05162 ko05164 ko05168 ko05169 map04138 map04140 map04141 map05160 map05162 map05164 map05168 map05169

Pfam Domains

Protein Families

HGTP_anticodon2 Pkinase RWD tRNA-synt_His

Protein Analysis

1237

Amino Acids

139.4

Weight (kDa)

5.7

Isoelectric Point (pI)

43.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWD	PF05773	35 - 144	1.6e-18	RWD domain
Pkinase	PF00069	418 - 723	8.5e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	527 - 720	4.2e-16	Protein tyrosine and serine/threonine kinase
tRNA-synt_His	PF13393	870 - 1112	1.4e-11	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	1133 - 1227	3.2e-09	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	1134 - 1223	2.3e-07	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59410 AT3G59410 AT3G59410 AT3G59410 AT3G59410
fragaria_vesca	FvH4_4g10800 FvH4_4g10800
malus_domestica	MD04G1040400.v1.1
prunus_persica	Prupe.1G194100_v2.0.a1 Prupe.1G194100_v2.0.a1
pyrus_communis	pycom04g03520
rosa_chinensis	RchiOBHm_Chr4g0406781
rosa_laevigata	RLG00000008759
rosa_multiflora	Rmu_sc0006408.1_g000006
rosa_roxburghii	Rroxscaffold_5G00351540
rosa_rugosa	Rorug04G0072900
rosa_samantha	Rh4AG140400 Rh4BG137400 Rh4CG147300 Rh4DG134400
rosa_wichuraiana	Rw4G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2156
Acc36I	ACCTGC	1 cut(s)	2664
AccB1I	GGYRCC	2 cut(s)	1992, 2019
AccI	GTMKAC	1 cut(s)	2616
AccII	CGCG	1 cut(s)	30
AccIII	TCCGGA	1 cut(s)	164
AciI	CCGC	6 cut(s)	30, 1348, 1381, 1908, 2104, 2846
AclWI	GGATC	9 cut(s)	234, 954, 967, 987, 1829, 1836, 3125, 3217, 3497
AcoI	YGGCCR	3 cut(s)	1288, 1800, 1931
AcsI	RAATTY	7 cut(s)	367, 425, 631, 902, 2078, 2604, 3648
AcuI	CTGAAG	3 cut(s)	132, 556, 3641
AcyI	GRCGYC	1 cut(s)	2920
AfaI	GTAC	7 cut(s)	1219, 1434, 1537, 1592, 1879, 1894, 1961
AfiI	CCNNNNNNNGG	3 cut(s)	311, 1354, 2103
AflII	CTTAAG	1 cut(s)	1352
AflIII	ACRYGT	2 cut(s)	1426, 3118
AhlI	ACTAGT	1 cut(s)	3448
AjnI	CCWGG	8 cut(s)	265, 1448, 1490, 1626, 1698, 3177, 3205, 3301
Alw21I	GWGCWC	5 cut(s)	172, 499, 1194, 1477, 3229
Alw26I	GTCTC	6 cut(s)	241, 611, 1010, 1272, 3159, 3326
Alw44I	GTGCAC	1 cut(s)	3225
AlwI	GGATC	9 cut(s)	234, 954, 967, 987, 1829, 1836, 3125, 3217, 3497
AlwNI	CAGNNNCTG	2 cut(s)	872, 3575
Ama87I	CYCGRG	1 cut(s)	378
Aor13HI	TCCGGA	1 cut(s)	164
AoxI	GGCC	8 cut(s)	197, 542, 743, 1288, 1446, 1696, 1800, 1931
ApaLI	GTGCAC	1 cut(s)	3225
ApeKI	GCWGC	5 cut(s)	416, 1508, 1822, 2542, 2999
ApoI	RAATTY	7 cut(s)	367, 425, 631, 902, 2078, 2604, 3648
AspA2I	CCTAGG	2 cut(s)	650, 1613
AspLEI	GCGC	1 cut(s)	30
AspS9I	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3315, 3604
AsuC2I	CCSGG	1 cut(s)	85
AsuHPI	GGTGA	8 cut(s)	175, 857, 1493, 2063, 2578, 2675, 2731, 3202
AsuII	TTCGAA	2 cut(s)	14, 2573
AvaI	CYCGRG	1 cut(s)	378
AvaII	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3315, 3604
AvrII	CCTAGG	2 cut(s)	650, 1613
AxyI	CCTNAGG	1 cut(s)	873
BaeGI	GKGCMC	1 cut(s)	3229
BaeI	ACNNNNGTAYC	2 cut(s)	254, 287
BalI	TGGCCA	3 cut(s)	1290, 1802, 1933
BamHI	GGATCC	1 cut(s)	959
BanI	GGYRCC	2 cut(s)	1992, 2019
BanII	GRGCYC	3 cut(s)	1194, 2870, 3034
BarI	GAAGNNNNNNTAC	6 cut(s)	2323, 2355, 2835, 2867, 3213, 3245
BauI	CACGAG	1 cut(s)	2376
BbsI	GAAGAC	2 cut(s)	2629, 2877
Bbv12I	GWGCWC	5 cut(s)	172, 499, 1194, 1477, 3229
BbvI	GCAGC	5 cut(s)	428, 1520, 1834, 2529, 2986
BccI	CCATC	9 cut(s)	194, 653, 1153, 1313, 1737, 1868, 2134, 2303, 2857
BceAI	ACGGC	1 cut(s)	2033
BcgI	CGANNNNNNTGC	2 cut(s)	1661, 1695
BciT130I	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
BciVI	GTATCC	1 cut(s)	282
BclI	TGATCA	2 cut(s)	358, 2712
BcnI	CCSGG	1 cut(s)	85
BcoDI	GTCTC	6 cut(s)	241, 611, 1010, 1272, 3159, 3326
BcuI	ACTAGT	1 cut(s)	3448
BfaI	CTAG	6 cut(s)	528, 651, 1316, 1614, 2195, 3449
BfmI	CTRYAG	8 cut(s)	1262, 1506, 1848, 2145, 2617, 2657, 3000, 3085
BfrI	CTTAAG	1 cut(s)	1352
BfuAI	ACCTGC	1 cut(s)	2664
BfuI	GTATCC	1 cut(s)	282
BglII	AGATCT	1 cut(s)	3079
BisI	GCNGC	5 cut(s)	417, 1509, 1823, 2543, 3000
BlnI	CCTAGG	2 cut(s)	650, 1613
BlpI	GCTNAGC	2 cut(s)	1543, 2282
BlsI	GCNGC	5 cut(s)	418, 1510, 1824, 2544, 3001
Bme1390I	CCNGG	9 cut(s)	85, 267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
Bme18I	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3315, 3604
BmeT110I	CYCGRG	1 cut(s)	378
BmgT120I	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3315, 3604
BmiI	GGNNCC	9 cut(s)	465, 543, 664, 961, 1994, 2021, 2869, 3031, 3316
BmrFI	CCNGG	9 cut(s)	85, 267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
BmsI	GCATC	6 cut(s)	1024, 1753, 2102, 2268, 2696, 3661
BpiI	GAAGAC	2 cut(s)	2629, 2877
BplI	GAGNNNNNCTC	2 cut(s)	1854, 1886
BpmI	CTGGAG	3 cut(s)	288, 1881, 2813
Bpu10I	CCTNAGC	2 cut(s)	194, 420
Bpu1102I	GCTNAGC	2 cut(s)	1543, 2282
Bpu14I	TTCGAA	2 cut(s)	14, 2573
BpuEI	CTTGAG	1 cut(s)	3115
BpuMI	CCSGG	1 cut(s)	85
BsaAI	YACGTR	1 cut(s)	1769
BsaBI	GATNNNNATC	2 cut(s)	1833, 3201
BsaHI	GRCGYC	1 cut(s)	2920
BsaI	GGTCTC	1 cut(s)	3326
BsaJI	CCNNGG	8 cut(s)	84, 266, 650, 942, 1491, 1613, 2509, 2779
BsaWI	WCCGGW	1 cut(s)	164
BsaXI	ACNNNNNCTCC	4 cut(s)	586, 616, 1250, 1280
Bsc4I	CCNNNNNNNGG	3 cut(s)	311, 1354, 2103
Bse1I	ACTGG	6 cut(s)	446, 467, 1864, 1885, 2796, 3580
Bse21I	CCTNAGG	1 cut(s)	873
Bse3DI	GCAATG	3 cut(s)	644, 2016, 3681
Bse8I	GATNNNNATC	2 cut(s)	1833, 3201
BseAI	TCCGGA	1 cut(s)	164
BseBI	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
BseDI	CCNNGG	8 cut(s)	84, 266, 650, 942, 1491, 1613, 2509, 2779
BseGI	GGATG	7 cut(s)	238, 664, 1166, 1725, 2093, 2467, 3676
BseJI	GATNNNNATC	2 cut(s)	1833, 3201
BseLI	CCNNNNNNNGG	3 cut(s)	311, 1354, 2103
BseMI	GCAATG	3 cut(s)	644, 2016, 3681
BseNI	ACTGG	6 cut(s)	446, 467, 1864, 1885, 2796, 3580
BseRI	GAGGAG	8 cut(s)	48, 51, 54, 57, 890, 1271, 2529, 3432
BseSI	GKGCMC	1 cut(s)	3229
BseXI	GCAGC	5 cut(s)	428, 1520, 1834, 2529, 2986
BsgI	GTGCAG	2 cut(s)	225, 997
Bsh1236I	CGCG	1 cut(s)	30
BshFI	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
BshNI	GGYRCC	2 cut(s)	1992, 2019
BsiHKAI	GWGCWC	5 cut(s)	172, 499, 1194, 1477, 3229
BsiHKCI	CYCGRG	1 cut(s)	378
BsiSI	CCGG	2 cut(s)	84, 165
BslI	CCNNNNNNNGG	3 cut(s)	311, 1354, 2103
BsmAI	GTCTC	6 cut(s)	241, 611, 1010, 1272, 3159, 3326
BsmBI	CGTCTC	1 cut(s)	1010
BsmI	GAATGC	2 cut(s)	2263, 3346
BsnI	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
Bso31I	GGTCTC	1 cut(s)	3326
BsoBI	CYCGRG	1 cut(s)	378
Bsp119I	TTCGAA	2 cut(s)	14, 2573
Bsp1286I	GDGCHC	7 cut(s)	172, 499, 1194, 1477, 2870, 3034, 3229
Bsp13I	TCCGGA	1 cut(s)	164
Bsp1407I	TGTACA	2 cut(s)	1590, 1959
Bsp1720I	GCTNAGC	2 cut(s)	1543, 2282
Bsp19I	CCATGG	1 cut(s)	2509
BspACI	CCGC	6 cut(s)	30, 1348, 1381, 1908, 2104, 2846
BspANI	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
BspEI	TCCGGA	1 cut(s)	164
BspFNI	CGCG	1 cut(s)	30
BspHI	TCATGA	2 cut(s)	949, 2305
BspLI	GGNNCC	9 cut(s)	465, 543, 664, 961, 1994, 2021, 2869, 3031, 3316
BspMAI	CTGCAG	4 cut(s)	1510, 1852, 2661, 3004
BspMI	ACCTGC	1 cut(s)	2664
BspPI	GGATC	9 cut(s)	234, 954, 967, 987, 1829, 1836, 3125, 3217, 3497
BspT104I	TTCGAA	2 cut(s)	14, 2573
BspT107I	GGYRCC	2 cut(s)	1992, 2019
BspTI	CTTAAG	1 cut(s)	1352
BspTNI	GGTCTC	1 cut(s)	3326
BsrDI	GCAATG	3 cut(s)	644, 2016, 3681
BsrGI	TGTACA	2 cut(s)	1590, 1959
BsrI	ACTGG	6 cut(s)	446, 467, 1864, 1885, 2796, 3580
BssECI	CCNNGG	8 cut(s)	84, 266, 650, 942, 1491, 1613, 2509, 2779
BssNI	GRCGYC	1 cut(s)	2920
BssSI	CACGAG	1 cut(s)	2376
BssT1I	CCWWGG	4 cut(s)	650, 942, 1613, 2509
Bst2BI	CACGAG	1 cut(s)	2376
Bst2UI	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
Bst4CI	ACNGT	5 cut(s)	494, 640, 1266, 2494, 2977
Bst6I	CTCTTC	4 cut(s)	108, 575, 780, 2268
BstACI	GRCGYC	1 cut(s)	2920
BstAFI	CTTAAG	1 cut(s)	1352
BstAPI	GCANNNNNTGC	2 cut(s)	2542, 3233
BstAUI	TGTACA	2 cut(s)	1590, 1959
BstBAI	YACGTR	1 cut(s)	1769
BstBI	TTCGAA	2 cut(s)	14, 2573
BstC8I	GCNNGC	6 cut(s)	708, 1012, 1367, 1513, 1931, 2163
BstDSI	CCRYGG	2 cut(s)	2509, 2779
BstF5I	GGATG	7 cut(s)	238, 664, 1166, 1725, 2093, 2467, 3676
BstFNI	CGCG	1 cut(s)	30
BstHHI	GCGC	1 cut(s)	30
BstMAI	GTCTC	6 cut(s)	241, 611, 1010, 1272, 3159, 3326
BstMWI	GCNNNNNNNGC	7 cut(s)	131, 167, 1930, 2072, 2542, 2548, 3233
BstNI	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
BstNSI	RCATGY	3 cut(s)	2165, 2639, 3122
BstSCI	CCNGG	9 cut(s)	83, 265, 1448, 1490, 1626, 1698, 3177, 3205, 3301
BstSFI	CTRYAG	8 cut(s)	1262, 1506, 1848, 2145, 2617, 2657, 3000, 3085
BstSLI	GKGCMC	1 cut(s)	3229
BstUI	CGCG	1 cut(s)	30
BstV1I	GCAGC	5 cut(s)	428, 1520, 1834, 2529, 2986
BstV2I	GAAGAC	2 cut(s)	2629, 2877
BstX2I	RGATCY	7 cut(s)	226, 959, 979, 1834, 3079, 3130, 3502
BstXI	CCANNNNNNTGG	1 cut(s)	2908
BstYI	RGATCY	7 cut(s)	226, 959, 979, 1834, 3079, 3130, 3502
Bsu36I	CCTNAGG	1 cut(s)	873
BsuI	GTATCC	1 cut(s)	282
BsuRI	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
BtgI	CCRYGG	2 cut(s)	2509, 2779
BtsCI	GGATG	7 cut(s)	238, 664, 1166, 1725, 2093, 2467, 3676
BtsI	GCAGTG	3 cut(s)	55, 120, 516
BtsIMutI	CAGTG	8 cut(s)	55, 120, 453, 499, 516, 580, 2490, 3573
BveI	ACCTGC	1 cut(s)	2664
Cac8I	GCNNGC	6 cut(s)	708, 1012, 1367, 1513, 1931, 2163
CaiI	CAGNNNCTG	2 cut(s)	872, 3575
CciI	TCATGA	2 cut(s)	949, 2305
CfoI	GCGC	1 cut(s)	30
Cfr13I	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3315, 3604
CseI	GACGC	3 cut(s)	150, 2441, 2909
Csp6I	GTAC	7 cut(s)	1218, 1433, 1536, 1591, 1878, 1893, 1960
CviQI	GTAC	7 cut(s)	1218, 1433, 1536, 1591, 1878, 1893, 1960
DraI	TTTAAA	5 cut(s)	720, 1125, 1531, 3364, 3691
EaeI	YGGCCR	3 cut(s)	1288, 1800, 1931
Eam1104I	CTCTTC	4 cut(s)	108, 575, 780, 2268
EarI	CTCTTC	4 cut(s)	108, 575, 780, 2268
EciI	GGCGGA	1 cut(s)	1337
Ecl136II	GAGCTC	1 cut(s)	1192
Eco130I	CCWWGG	4 cut(s)	650, 942, 1613, 2509
Eco147I	AGGCCT	3 cut(s)	199, 1448, 1698
Eco24I	GRGCYC	3 cut(s)	1194, 2870, 3034
Eco31I	GGTCTC	1 cut(s)	3326
Eco32I	GATATC	1 cut(s)	1241
Eco47I	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3315, 3604
Eco53kI	GAGCTC	1 cut(s)	1192
Eco57I	CTGAAG	3 cut(s)	132, 556, 3641
Eco81I	CCTNAGG	1 cut(s)	873
Eco88I	CYCGRG	1 cut(s)	378
EcoICRI	GAGCTC	1 cut(s)	1192
EcoO109I	RGGNCCY	1 cut(s)	542
EcoRI	GAATTC	1 cut(s)	902
EcoRII	CCWGG	8 cut(s)	265, 1448, 1490, 1626, 1698, 3177, 3205, 3301
EcoRV	GATATC	1 cut(s)	1241
EcoT14I	CCWWGG	4 cut(s)	650, 942, 1613, 2509
EcoT22I	ATGCAT	2 cut(s)	678, 2167
EcoT38I	GRGCYC	3 cut(s)	1194, 2870, 3034
ErhI	CCWWGG	4 cut(s)	650, 942, 1613, 2509
Esp3I	CGTCTC	1 cut(s)	1010
FalI	AAGNNNNNCTT	2 cut(s)	53, 85
FauNDI	CATATG	1 cut(s)	3270
FbaI	TGATCA	2 cut(s)	358, 2712
FblI	GTMKAC	1 cut(s)	2616
Fnu4HI	GCNGC	5 cut(s)	417, 1509, 1823, 2543, 3000
FokI	GGATG	7 cut(s)	245, 671, 1153, 1712, 2080, 2474, 3683
FriOI	GRGCYC	3 cut(s)	1194, 2870, 3034
Fsp4HI	GCNGC	5 cut(s)	417, 1509, 1823, 2543, 3000
FspBI	CTAG	6 cut(s)	528, 651, 1316, 1614, 2195, 3449
GlaI	GCGC	1 cut(s)	29
GluI	GCNGC	5 cut(s)	417, 1509, 1823, 2543, 3000
GsuI	CTGGAG	3 cut(s)	288, 1881, 2813
HaeIII	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
HapII	CCGG	2 cut(s)	84, 165
HgaI	GACGC	3 cut(s)	150, 2441, 2909
HhaI	GCGC	1 cut(s)	30
Hin1I	GRCGYC	1 cut(s)	2920
Hin6I	GCGC	1 cut(s)	28
HinP1I	GCGC	1 cut(s)	28
HincII	GTYRAC	1 cut(s)	613
HindII	GTYRAC	1 cut(s)	613
HindIII	AAGCTT	2 cut(s)	191, 2284
HpaII	CCGG	2 cut(s)	84, 165
HphI	GGTGA	8 cut(s)	175, 857, 1493, 2063, 2578, 2675, 2731, 3202
Hpy166II	GTNNAC	4 cut(s)	613, 2054, 2617, 3227
Hpy8I	GTNNAC	4 cut(s)	613, 2054, 2617, 3227
HpyCH4III	ACNGT	5 cut(s)	494, 640, 1266, 2494, 2977
HpyCH4IV	ACGT	3 cut(s)	1428, 1768, 2875
HpyF10VI	GCNNNNNNNGC	7 cut(s)	131, 167, 1930, 2072, 2542, 2548, 3233
HpySE526I	ACGT	3 cut(s)	1428, 1768, 2875
Hsp92I	GRCGYC	1 cut(s)	2920
HspAI	GCGC	1 cut(s)	28
Kpn2I	TCCGGA	1 cut(s)	164
Ksp22I	TGATCA	2 cut(s)	358, 2712
Lsp1109I	GCAGC	5 cut(s)	428, 1520, 1834, 2529, 2986
LweI	GCATC	6 cut(s)	1024, 1753, 2102, 2268, 2696, 3661
MaeI	CTAG	6 cut(s)	528, 651, 1316, 1614, 2195, 3449
MaeII	ACGT	3 cut(s)	1428, 1768, 2875
MaeIII	GTNAC	6 cut(s)	326, 530, 2719, 2734, 3009, 3214
MfeI	CAATTG	3 cut(s)	2628, 2749, 3237
MflI	RGATCY	7 cut(s)	226, 959, 979, 1834, 3079, 3130, 3502
MhlI	GDGCHC	7 cut(s)	172, 499, 1194, 1477, 2870, 3034, 3229
MlsI	TGGCCA	3 cut(s)	1290, 1802, 1933
MluNI	TGGCCA	3 cut(s)	1290, 1802, 1933
MlyI	GAGTC	4 cut(s)	323, 917, 1646, 2826
MmeI	TCCRAC	6 cut(s)	578, 801, 900, 1797, 2058, 2439
Mox20I	TGGCCA	3 cut(s)	1290, 1802, 1933
Mph1103I	ATGCAT	2 cut(s)	678, 2167
MroI	TCCGGA	1 cut(s)	164
MscI	TGGCCA	3 cut(s)	1290, 1802, 1933
MslI	CAYNNNNRTG	3 cut(s)	1598, 1710, 2307
Msp20I	TGGCCA	3 cut(s)	1290, 1802, 1933
MspA1I	CMGCKG	1 cut(s)	1825
MspCI	CTTAAG	1 cut(s)	1352
MspI	CCGG	2 cut(s)	84, 165
MspR9I	CCNGG	9 cut(s)	85, 267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
MunI	CAATTG	3 cut(s)	2628, 2749, 3237
Mva1269I	GAATGC	2 cut(s)	2263, 3346
MvaI	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
MvnI	CGCG	1 cut(s)	30
MwoI	GCNNNNNNNGC	7 cut(s)	131, 167, 1930, 2072, 2542, 2548, 3233
NciI	CCSGG	1 cut(s)	85
NcoI	CCATGG	1 cut(s)	2509
NdeI	CATATG	1 cut(s)	3270
NlaIV	GGNNCC	9 cut(s)	465, 543, 664, 961, 1994, 2021, 2869, 3031, 3316
NmuCI	GTSAC	3 cut(s)	326, 2719, 3009
NsiI	ATGCAT	2 cut(s)	678, 2167
NspI	RCATGY	3 cut(s)	2165, 2639, 3122
NspV	TTCGAA	2 cut(s)	14, 2573
PaeI	GCATGC	1 cut(s)	2165
PagI	TCATGA	2 cut(s)	949, 2305
PceI	AGGCCT	3 cut(s)	199, 1448, 1698
PciI	ACATGT	1 cut(s)	3118
PctI	GAATGC	2 cut(s)	2263, 3346
PfeI	GAWTC	9 cut(s)	682, 803, 881, 1577, 1722, 2183, 2347, 2366, 2575
PfoI	TCCNGGA	1 cut(s)	3205
PkrI	GCNGC	5 cut(s)	418, 1510, 1824, 2544, 3001
PleI	GAGTC	4 cut(s)	323, 917, 1645, 2825
PpsI	GAGTC	4 cut(s)	323, 917, 1645, 2825
Ppu21I	YACGTR	1 cut(s)	1769
PscI	ACATGT	1 cut(s)	3118
PsiI	TTATAA	1 cut(s)	2156
Psp124BI	GAGCTC	1 cut(s)	1194
Psp6I	CCWGG	8 cut(s)	265, 1448, 1490, 1626, 1698, 3177, 3205, 3301
PspGI	CCWGG	8 cut(s)	265, 1448, 1490, 1626, 1698, 3177, 3205, 3301
PspN4I	GGNNCC	9 cut(s)	465, 543, 664, 961, 1994, 2021, 2869, 3031, 3316
PspPI	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3315, 3604
PsrI	GAACNNNNNNTAC	2 cut(s)	2928, 2960
PstI	CTGCAG	4 cut(s)	1510, 1852, 2661, 3004
PstNI	CAGNNNCTG	2 cut(s)	872, 3575
PsuI	RGATCY	7 cut(s)	226, 959, 979, 1834, 3079, 3130, 3502
PvuII	CAGCTG	1 cut(s)	1825
RsaI	GTAC	7 cut(s)	1219, 1434, 1537, 1592, 1879, 1894, 1961
RsaNI	GTAC	7 cut(s)	1218, 1433, 1536, 1591, 1878, 1893, 1960
RseI	CAYNNNNRTG	3 cut(s)	1598, 1710, 2307
SacI	GAGCTC	1 cut(s)	1194
SatI	GCNGC	5 cut(s)	417, 1509, 1823, 2543, 3000
Sau96I	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3315, 3604
SbfI	CCTGCAGG	1 cut(s)	2661
SchI	GAGTC	4 cut(s)	323, 917, 1646, 2826
ScrFI	CCNGG	9 cut(s)	85, 267, 1450, 1492, 1628, 1700, 3179, 3207, 3303
SdaI	CCTGCAGG	1 cut(s)	2661
SduI	GDGCHC	7 cut(s)	172, 499, 1194, 1477, 2870, 3034, 3229
SfaNI	GCATC	6 cut(s)	1024, 1753, 2102, 2268, 2696, 3661
SfcI	CTRYAG	8 cut(s)	1262, 1506, 1848, 2145, 2617, 2657, 3000, 3085
SfuI	TTCGAA	2 cut(s)	14, 2573
SinI	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3315, 3604
SmiMI	CAYNNNNRTG	3 cut(s)	1598, 1710, 2307
SmlI	CTYRAG	2 cut(s)	1352, 3094
SmoI	CTYRAG	2 cut(s)	1352, 3094
SpeI	ACTAGT	1 cut(s)	3448
SphI	GCATGC	1 cut(s)	2165
Sse8387I	CCTGCAGG	1 cut(s)	2661
SseBI	AGGCCT	3 cut(s)	199, 1448, 1698
SsiI	CCGC	6 cut(s)	30, 1348, 1381, 1908, 2104, 2846
SspI	AATATT	2 cut(s)	138, 2203
SspMI	CTAG	6 cut(s)	528, 651, 1316, 1614, 2195, 3449
SstI	GAGCTC	1 cut(s)	1194
StuI	AGGCCT	3 cut(s)	199, 1448, 1698
StyD4I	CCNGG	9 cut(s)	83, 265, 1448, 1490, 1626, 1698, 3177, 3205, 3301
StyI	CCWWGG	4 cut(s)	650, 942, 1613, 2509
TaaI	ACNGT	5 cut(s)	494, 640, 1266, 2494, 2977
TaiI	ACGT	3 cut(s)	1431, 1771, 2878
TaqI	TCGA	9 cut(s)	14, 320, 411, 1129, 1237, 1872, 1983, 2573, 2670
TatI	WGTACW	3 cut(s)	1217, 1590, 1959
TfiI	GAWTC	9 cut(s)	682, 803, 881, 1577, 1722, 2183, 2347, 2366, 2575
TscAI	CASTG	8 cut(s)	55, 127, 453, 499, 523, 580, 2497, 3580
TseFI	GTSAC	3 cut(s)	326, 2719, 3009
TseI	GCWGC	5 cut(s)	416, 1508, 1822, 2542, 2999
Tsp45I	GTSAC	3 cut(s)	326, 2719, 3009
TspGWI	ACGGA	5 cut(s)	992, 2908, 3070, 3530, 3596
TspRI	CASTG	8 cut(s)	55, 127, 453, 499, 523, 580, 2497, 3580
Vha464I	CTTAAG	1 cut(s)	1352
VneI	GTGCAC	1 cut(s)	3225
VpaK11BI	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3315, 3604
XapI	RAATTY	7 cut(s)	367, 425, 631, 902, 2078, 2604, 3648
XceI	RCATGY	3 cut(s)	2165, 2639, 3122
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2455
XmaJI	CCTAGG	2 cut(s)	650, 1613
XmiI	GTMKAC	1 cut(s)	2616
XspI	CTAG	6 cut(s)	528, 651, 1316, 1614, 2195, 3449
Zsp2I	ATGCAT	2 cut(s)	678, 2167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.