Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0010438

Physical Location & Seq

Genomic Coordinates Reverse (-)

24302512 .. 24494012

Gene Structure 191501 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG137400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3945 bp

ATGACCCTCGGGTGTGTCGTCGTTGAGCTCCCAGATGAATTCGTTAGGGAAAGCTCTGTAGTTGTACTGGTCGACCTCGGTGTCGAGCTTCTTCATCCACCCGACTTTGATGAAGAAGTTGGTGAAGTCCTGGTTAACCTTTTCGAAGAATATCCTCTTCTGGACGGAGTAGCCGAACCTGTTGTTGCTGTGCTGTCTCCAGAGGTCGTCAATGGCTTTGAGGTCGGCTTCGGGGATGAACTGGACCTCGGAGAAAAAGACGTATCCTCGCTTTTGGGCTGCCTCACCTGCGAGGACGATGAGGAGGCGGCGAGTCTCTTCGTCGGCTTCGCGGAAGTTTTGGGCGGAGAGGTGCTGCTGTGTGCAATATTTCAAGATGACTGCAAAATTGCGTCCGGATCACAACCTCAAATCACCATCAAGCTTAGGCCTCACTCAAAGGATATGGGTTATGAGGATCTGGATGTCTCTGCACTTCTTTTAGTTAGGTGCTTGCCAGGGTATCCTTACAAGTGTCCCAAGTTGCAGATAACTCCAGAGAAAGGATTATCGAAAAGTGACTCTGATAAGCTATTATCTCTTATCAATGATCAGGCAAATTCCAATGCCCGAGAAGGACGGGTAATGATCTTCAATTTGGTCGAGGCAGCTCAGGAATTTCTATCTGAAATTGTGCCAGTGGGTCAATCACAGGGACCGGTAATATGTTCAACTACGGATAACAGTGCTCAGTTGTTTCAAAATGACACTGCAATTTCTAGTAACAAGAAGGGGCCTTTTGTTTATGGTTTCATAGACCTCTTCAGTGGCTCTGGAAAGTCTTGGAGTTGGAGTTTTGGAGTTGACGAGACTAAAGGGATAAATTCTACTGTCCCATTGCCTAGGGTGGATGGGTCCAAACTCATGCATGAGATTCAGGAAAAGAAACATGATAAGCAAGCAGAACCTTTAAAATCACAAGATATAAAGCAAGGGCCAGTACTTTCCCCTAATGTCAAGTTAGAAACGCTTGAAGAAGAGAGTGAAGGCAGGGATTCAAGTAGATTTTTGTTGGAAGAAATGGACAGAAATGGTGGTGAAACTGAGAAAGAGAGTTCAGTTCTTGAGGAGGATTCAACAGAAGATGATTGGGAATTCGGAAGTCAGCAGTCGGACTCGCTCTCTTTTACTTCCTTGGCTCATGAGCAAGGATCCCAAAATGTTAAAAGGGATCTAATAATGGTTCATCTGCTCCGTCTCGCCTGCACTAAAGGACCATTGGCTGATGCTTTGCCACAGATAACCACAGAACTGCAAAACATAGGAGTCTTATCAGAATGGGCAAGGGATTTAGCTTCAAAACCAATTTCACTTTTAAATCGAACATTTAATCATGCCTTCGGACAACAGATGGTTTCATCCCGTATCTCTCAATTCTGGGAGCTCACTTCTGATTTAGAAGGACCAAGTACATCTCTCCCAAGTTCTCGATATCTCAATGACTTTGAGGAGCTACAGTCTCTTGGTCATGGTGGCTTTGGCCATGTTGTGTTGTGCAAAAATAAGCTAGATGGAAGGCAGTATGCAGTAAAGAAAATCCGCCTTAAGGATAAAAGCCTGCCACTTGATGACCGCATATTGAGGGAAGTCGCTACACTTTCTCGTTTGCAACATCAACACGTTGTACGATACTATCAGGCCTGGTTTGAAACAGGAGGTGTTGGTGCTCATGGTGATACTACCTGGGGTTCAAGGACTGCAGCCAGCTCCACTTTTAGCTTTAAAGGTACAAGCTCAGCAGATGATCTTGGGAATGAGAATAAACTTGAATCAACATATTTGTACATTCAAATGGAGTACTGCCCTAGGACATTACGCCAGGTTTTTGAGTCGTATAGTCAATTTGACAAAGAATTAGCATGGCATTTGTTTCGTCAAATTGTTGAAGGCCTGGCACACATACATGGACAAGGAATCATCCATCGGGACTTAACGCCAAATAACATATTCTTTGATGCACGTAATGATATTAAAATTGGAGATTTTGGTTTGGCCAAGTTTTTGAAGTTGGAGCAGCTGGATCAAGATCCAAGTTTTCCTGCAGATACAACTGGAGTTTCTCTTGATGGTACTGGTCAAGTTGGTACATATTTTTACACCGCACCTGAAATTGAGCAAGGCTGGCCAAAGATTGATGAAAAGGCTGATATGTACAGCTTGGGAATTGTATTTTTCGAGCTATGGCACCCATTTGGGACTGCAATGGAACGGCACCTTGTCCTATCTGACTTGAAACAGAATGGTGAACTGCCTTCTGCTTGGGTTGCTGAATTTCCGGAACAAGCATCCTTGTTGCGGAGCTTAATGTCCCCAAGTCCGTCAGATCGCCCTTCTGCTACAGAACTTATAAAGCATGCATTTCCACCACGAATGGAATCTGAGTTGCTAGACAATATTCTACGAACAATGCAAACCTCAGAGGACAGAAGTGTGTATGATAAAGTTCTGAATGCTATATTTGATGAAGAGATGCTAAGCTTGAAAGACCAGCAACATCATGATGGTAGGTTGAGGTTGGCTGGTAGCGATACTTCTGCGATTCAGTATGCAGATTTAGATTCGGAGGCTCGTGATTATGTTGTTGAGATCACAAGGGAGGTGTTCAGACAACATTGTGCGAAGCACCTTGAAGTAATACCCATGCGTCTGTTGGATGATTGCCAACATTTTATGAGAAGGAACACTGTCAAACTTTTGACCCATGGAGGAGATATGCTTGAACTTTTGCATGAGCTGCGTTTGCCTTTTGTTAGTTGGGTGATTTCGAATCAGAAATCTTCATTCAAAAGATATGAAATTTCATGTGTCTACAGAAGACCAATTGGACATGCACCCCCAAGTCGCTACCTGCAGGGTGATTTCGACATTATTGGAGGTGCATCAGCATTGACAGAGGCAGAAGTGATCAAGGTGACAAGGGACATTATAACCCGTTTTTTCAATTGGGAGTTCTGCGACATTCATCTAAACCACGGGGACTTACTGGAGGCAATTTGGTCTTGGGTAGGAGTCAAGGCAGACCGTAGACAAAAAGTAGCGGAGCTTCTTTCTATGATGGGCTCCTTACGTCCTCAGTCTTCTGAACGGAAATCCAAATGGGTGGTAATAAGGCGTCAGCTTTTGCAGGAACTTAATTTACAAGAGGCTGTTGTGAATAGGTTGCAGACTGTTGGTTTAAGGTTCTGTGGAGCTGCAGATCAGGCACTTCCTAGACTTAGGGGTGCCCTGCCAAACGATAAACCAACACGGAAGGCACTGGATGAGTTGTCAGATCTCTGTAGCTACTTGAGAGCTTGGAGGATTGAAGAACATGTTTATATAGATCCACTTATCCCCCCAACTGAGAGTTATCATAGAGACTTGTTTTTCCAGGTATATTTGGTGAAGGATAGTAATCCTGGATCACTTACCGAAGGTGCACTACTTGCAATTGGGGGTCGGTATGACTATTTGCTTCATCATATGTGGGGTCTTGAACATAAATCTAATCCTCCTGGTTCTGTTGGGACCAGCCTTGCTTTAGAGACCATAATTCAGCATTCTCCTGTGGATTTTAAACCAATTAGCAGAAATGAAGTTAGCAATAGTGTTCTTGTTTGTTCAAAAGGAGGAGGGGGTCTATTGGCAGAACGCATGGAACTAGTCAATGAGCTTTGGGAAGAAAACATTAAGGCTGAGTTTCTCCCAACACCAGATCCAAGCCTTACGGAGCAGTATGAATATGCCAACGAACATGATATTAAATGTCTTGTCATCATAACAGACACTGGTGTTTCTCAGAAAGGTTCTGTTAAGGTCCGTCATCTTGAACTGAAGAAGGAAAAGGAGGTTGAAAGGGAAATTCTCGTCAGGTTTCTGCTGGATGCAATGGCAATACAATTTAAAAATCCTTCCATTTGGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0014070 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034198 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0046677 GO:0050896 GO:0051716 GO:0060992 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928

KEGG Pathways

Metabolic & Signaling

ko04138 ko04140 ko04141 ko05160 ko05162 ko05164 ko05168 ko05169 map04138 map04140 map04141 map05160 map05162 map05164 map05168 map05169

Pfam Domains

Protein Families

HGTP_anticodon2 Pkinase RWD tRNA-synt_His

Protein Analysis

1314

Amino Acids

147.73

Weight (kDa)

5.02

Isoelectric Point (pI)

41.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWD	PF05773	120 - 221	8.2e-16	RWD domain
Pkinase	PF00069	495 - 800	1.1e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	605 - 797	4.8e-16	Protein tyrosine and serine/threonine kinase
tRNA-synt_His	PF13393	947 - 1189	6.2e-11	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	1210 - 1304	3.4e-09	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	1211 - 1300	2.4e-07	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59410 AT3G59410 AT3G59410 AT3G59410 AT3G59410
fragaria_vesca	FvH4_4g10800 FvH4_4g10800
malus_domestica	MD04G1040400.v1.1
prunus_persica	Prupe.1G194100_v2.0.a1 Prupe.1G194100_v2.0.a1
pyrus_communis	pycom04g03520
rosa_chinensis	RchiOBHm_Chr4g0406781
rosa_laevigata	RLG00000008759
rosa_multiflora	Rmu_sc0006408.1_g000006
rosa_roxburghii	Rroxscaffold_5G00351540
rosa_rugosa	Rorug04G0072900
rosa_samantha	Rh4AG140400 Rh4BG137400 Rh4CG147300 Rh4DG134400
rosa_wichuraiana	Rw4G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2387, 2966
AarI	CACCTGC	1 cut(s)	296
AasI	GACNNNNNNGTC	1 cut(s)	80
Acc36I	ACCTGC	2 cut(s)	296, 2895
AccB1I	GGYRCC	3 cut(s)	2223, 2250, 3260
AccI	GTMKAC	3 cut(s)	72, 2847, 3064
AccII	CGCG	1 cut(s)	332
AccIII	TCCGGA	2 cut(s)	395, 2314
AciI	CCGC	8 cut(s)	308, 332, 345, 1579, 1612, 2139, 2335, 3077
AcoI	YGGCCR	3 cut(s)	1519, 2031, 2162
AcsI	RAATTY	8 cut(s)	38, 598, 656, 862, 1133, 2309, 2835, 3879
AcuI	CTGAAG	2 cut(s)	787, 3872
AcyI	GRCGYC	1 cut(s)	3151
AfiI	CCNNNNNNNGG	3 cut(s)	542, 1585, 2334
AflII	CTTAAG	1 cut(s)	1583
AflIII	ACRYGT	2 cut(s)	1657, 3349
AgeI	ACCGGT	1 cut(s)	697
AhlI	ACTAGT	1 cut(s)	3679
AjnI	CCWGG	9 cut(s)	129, 496, 1679, 1721, 1857, 1929, 3408, 3436, 3532
Alw21I	GWGCWC	5 cut(s)	30, 730, 1425, 1708, 3460
Alw26I	GTCTC	8 cut(s)	201, 320, 472, 842, 1241, 1503, 3390, 3557
Alw44I	GTGCAC	1 cut(s)	3456
AlwNI	CAGNNNCTG	1 cut(s)	3806
Ama87I	CYCGRG	2 cut(s)	8, 609
Aor13HI	TCCGGA	2 cut(s)	395, 2314
AoxI	GGCC	8 cut(s)	428, 773, 974, 1519, 1677, 1927, 2031, 2162
ApaLI	GTGCAC	1 cut(s)	3456
ApeKI	GCWGC	7 cut(s)	279, 355, 647, 1739, 2053, 2773, 3230
ApoI	RAATTY	8 cut(s)	38, 598, 656, 862, 1133, 2309, 2835, 3879
AsiGI	ACCGGT	1 cut(s)	697
AspA2I	CCTAGG	2 cut(s)	881, 1844
AspS9I	GGNCC	9 cut(s)	244, 695, 773, 894, 974, 1253, 1442, 3546, 3835
AsuII	TTCGAA	2 cut(s)	144, 2804
AvaI	CYCGRG	2 cut(s)	8, 609
AvaII	GGWCC	7 cut(s)	244, 695, 894, 1253, 1442, 3546, 3835
AvrII	CCTAGG	2 cut(s)	881, 1844
BaeGI	GKGCMC	2 cut(s)	3265, 3460
BalI	TGGCCA	3 cut(s)	1521, 2033, 2164
BamHI	GGATCC	1 cut(s)	1190
BanI	GGYRCC	3 cut(s)	2223, 2250, 3260
BanII	GRGCYC	3 cut(s)	30, 1425, 3101
BarI	GAAGNNNNNNTAC	6 cut(s)	2554, 2586, 3066, 3098, 3444, 3476
BauI	CACGAG	1 cut(s)	2607
BbsI	GAAGAC	2 cut(s)	2860, 3108
Bbv12I	GWGCWC	5 cut(s)	30, 730, 1425, 1708, 3460
BbvI	GCAGC	7 cut(s)	266, 342, 659, 1751, 2065, 2760, 3217
BccI	CCATC	8 cut(s)	425, 884, 1384, 1544, 1968, 2099, 2534, 3088
BceAI	ACGGC	1 cut(s)	2264
BcgI	CGANNNNNNTGC	4 cut(s)	1892, 1926, 2555, 2589
BciT130I	CCWGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
BciVI	GTATCC	2 cut(s)	274, 513
BclI	TGATCA	2 cut(s)	589, 2943
BcoDI	GTCTC	8 cut(s)	201, 320, 472, 842, 1241, 1503, 3390, 3557
BcuI	ACTAGT	1 cut(s)	3679
BfaI	CTAG	7 cut(s)	759, 882, 1547, 1845, 2426, 3249, 3680
BfmI	CTRYAG	9 cut(s)	57, 1493, 1737, 2079, 2376, 2848, 2888, 3231, 3316
BfrI	CTTAAG	1 cut(s)	1583
BfuAI	ACCTGC	2 cut(s)	296, 2895
BfuI	GTATCC	2 cut(s)	274, 513
BglII	AGATCT	1 cut(s)	3310
BisI	GCNGC	8 cut(s)	280, 309, 356, 648, 1740, 2054, 2774, 3231
BlnI	CCTAGG	2 cut(s)	881, 1844
BlpI	GCTNAGC	2 cut(s)	1774, 2513
BlsI	GCNGC	8 cut(s)	281, 310, 357, 649, 1741, 2055, 2775, 3232
BmcAI	AGTACT	2 cut(s)	981, 1838
Bme1390I	CCNGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
Bme18I	GGWCC	7 cut(s)	244, 695, 894, 1253, 1442, 3546, 3835
BmeT110I	CYCGRG	2 cut(s)	8, 609
BmgT120I	GGNCC	9 cut(s)	244, 695, 773, 894, 974, 1253, 1442, 3546, 3835
BmiI	GGNNCC	9 cut(s)	696, 774, 895, 1192, 2225, 2252, 3100, 3262, 3547
BmrFI	CCNGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
BmsI	GCATC	6 cut(s)	1255, 1984, 2333, 2499, 2927, 3892
BpiI	GAAGAC	2 cut(s)	2860, 3108
BpmI	CTGGAG	4 cut(s)	183, 519, 2112, 3044
Bpu10I	CCTNAGC	2 cut(s)	425, 651
Bpu1102I	GCTNAGC	2 cut(s)	1774, 2513
Bpu14I	TTCGAA	2 cut(s)	144, 2804
BpuEI	CTTGAG	2 cut(s)	1124, 3346
BsaAI	YACGTR	1 cut(s)	2000
BsaBI	GATNNNNATC	2 cut(s)	2064, 3432
BsaHI	GRCGYC	1 cut(s)	3151
BsaI	GGTCTC	1 cut(s)	3557
BsaWI	WCCGGW	3 cut(s)	395, 697, 2314
BsaXI	ACNNNNNCTCC	4 cut(s)	817, 847, 1481, 1511
Bsc4I	CCNNNNNNNGG	3 cut(s)	542, 1585, 2334
Bse118I	RCCGGY	1 cut(s)	697
Bse1I	ACTGG	9 cut(s)	72, 246, 677, 977, 2095, 2116, 3027, 3300, 3811
Bse3DI	GCAATG	3 cut(s)	875, 2247, 3912
Bse8I	GATNNNNATC	2 cut(s)	2064, 3432
BseAI	TCCGGA	2 cut(s)	395, 2314
BseBI	CCWGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
BseJI	GATNNNNATC	2 cut(s)	2064, 3432
BseLI	CCNNNNNNNGG	3 cut(s)	542, 1585, 2334
BseMI	GCAATG	3 cut(s)	875, 2247, 3912
BseNI	ACTGG	9 cut(s)	72, 246, 677, 977, 2095, 2116, 3027, 3300, 3811
BseRI	GAGGAG	5 cut(s)	317, 1121, 1502, 2760, 3663
BseSI	GKGCMC	2 cut(s)	3265, 3460
BseXI	GCAGC	7 cut(s)	266, 342, 659, 1751, 2065, 2760, 3217
BsgI	GTGCAG	2 cut(s)	456, 1228
Bsh1236I	CGCG	1 cut(s)	332
BshFI	GGCC	8 cut(s)	430, 775, 976, 1521, 1679, 1929, 2033, 2164
BshNI	GGYRCC	3 cut(s)	2223, 2250, 3260
BshTI	ACCGGT	1 cut(s)	697
BsiHKAI	GWGCWC	5 cut(s)	30, 730, 1425, 1708, 3460
BsiHKCI	CYCGRG	2 cut(s)	8, 609
BsiSI	CCGG	3 cut(s)	396, 698, 2315
BslFI	GGGAC	9 cut(s)	501, 708, 857, 1979, 2248, 2332, 2972, 3029, 3559
BslI	CCNNNNNNNGG	3 cut(s)	542, 1585, 2334
BsmAI	GTCTC	8 cut(s)	201, 320, 472, 842, 1241, 1503, 3390, 3557
BsmBI	CGTCTC	1 cut(s)	1241
BsmFI	GGGAC	9 cut(s)	501, 708, 857, 1979, 2248, 2332, 2972, 3029, 3559
BsmI	GAATGC	2 cut(s)	2494, 3577
BsnI	GGCC	8 cut(s)	430, 775, 976, 1521, 1679, 1929, 2033, 2164
Bso31I	GGTCTC	1 cut(s)	3557
BsoBI	CYCGRG	2 cut(s)	8, 609
Bsp119I	TTCGAA	2 cut(s)	144, 2804
Bsp1286I	GDGCHC	7 cut(s)	30, 730, 1425, 1708, 3101, 3265, 3460
Bsp13I	TCCGGA	2 cut(s)	395, 2314
Bsp1407I	TGTACA	2 cut(s)	1821, 2190
Bsp1720I	GCTNAGC	2 cut(s)	1774, 2513
Bsp19I	CCATGG	1 cut(s)	2740
BspACI	CCGC	8 cut(s)	308, 332, 345, 1579, 1612, 2139, 2335, 3077
BspANI	GGCC	8 cut(s)	430, 775, 976, 1521, 1679, 1929, 2033, 2164
BspEI	TCCGGA	2 cut(s)	395, 2314
BspFNI	CGCG	1 cut(s)	332
BspHI	TCATGA	2 cut(s)	1180, 2536
BspLI	GGNNCC	9 cut(s)	696, 774, 895, 1192, 2225, 2252, 3100, 3262, 3547
BspMAI	CTGCAG	4 cut(s)	1741, 2083, 2892, 3235
BspMI	ACCTGC	2 cut(s)	296, 2895
BspT104I	TTCGAA	2 cut(s)	144, 2804
BspT107I	GGYRCC	3 cut(s)	2223, 2250, 3260
BspTI	CTTAAG	1 cut(s)	1583
BspTNI	GGTCTC	1 cut(s)	3557
BsrDI	GCAATG	3 cut(s)	875, 2247, 3912
BsrFI	RCCGGY	1 cut(s)	697
BsrGI	TGTACA	2 cut(s)	1821, 2190
BsrI	ACTGG	9 cut(s)	72, 246, 677, 977, 2095, 2116, 3027, 3300, 3811
BssAI	RCCGGY	1 cut(s)	697
BssNI	GRCGYC	1 cut(s)	3151
BssSI	CACGAG	1 cut(s)	2607
BssT1I	CCWWGG	4 cut(s)	881, 1173, 1844, 2740
Bst2BI	CACGAG	1 cut(s)	2607
Bst2UI	CCWGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
Bst4CI	ACNGT	6 cut(s)	725, 871, 1497, 2725, 3062, 3208
Bst6I	CTCTTC	5 cut(s)	162, 323, 806, 1011, 2499
BstACI	GRCGYC	1 cut(s)	3151
BstAFI	CTTAAG	1 cut(s)	1583
BstAPI	GCANNNNNTGC	2 cut(s)	2773, 3464
BstAUI	TGTACA	2 cut(s)	1821, 2190
BstBAI	YACGTR	1 cut(s)	2000
BstBI	TTCGAA	2 cut(s)	144, 2804
BstC8I	GCNNGC	7 cut(s)	494, 939, 1243, 1598, 1744, 2162, 2394
BstDSI	CCRYGG	2 cut(s)	2740, 3010
BstFNI	CGCG	1 cut(s)	332
BstMAI	GTCTC	8 cut(s)	201, 320, 472, 842, 1241, 1503, 3390, 3557
BstMWI	GCNNNNNNNGC	7 cut(s)	288, 2161, 2303, 2773, 2779, 3239, 3464
BstNI	CCWGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
BstNSI	RCATGY	3 cut(s)	2396, 2870, 3353
BstSCI	CCNGG	9 cut(s)	129, 496, 1679, 1721, 1857, 1929, 3408, 3436, 3532
BstSFI	CTRYAG	9 cut(s)	57, 1493, 1737, 2079, 2376, 2848, 2888, 3231, 3316
BstSLI	GKGCMC	2 cut(s)	3265, 3460
BstUI	CGCG	1 cut(s)	332
BstV1I	GCAGC	7 cut(s)	266, 342, 659, 1751, 2065, 2760, 3217
BstV2I	GAAGAC	2 cut(s)	2860, 3108
BstX2I	RGATCY	7 cut(s)	457, 1190, 1210, 2065, 3310, 3361, 3733
BstXI	CCANNNNNNTGG	1 cut(s)	3139
BstYI	RGATCY	7 cut(s)	457, 1190, 1210, 2065, 3310, 3361, 3733
BsuI	GTATCC	2 cut(s)	274, 513
BsuRI	GGCC	8 cut(s)	430, 775, 976, 1521, 1679, 1929, 2033, 2164
BtgI	CCRYGG	2 cut(s)	2740, 3010
BtsI	GCAGTG	1 cut(s)	747
BtsIMutI	CAGTG	7 cut(s)	684, 730, 747, 811, 2721, 3293, 3804
BveI	ACCTGC	2 cut(s)	296, 2895
Cac8I	GCNNGC	7 cut(s)	494, 939, 1243, 1598, 1744, 2162, 2394
CaiI	CAGNNNCTG	1 cut(s)	3806
CciI	TCATGA	2 cut(s)	1180, 2536
Cfr10I	RCCGGY	1 cut(s)	697
Cfr13I	GGNCC	9 cut(s)	244, 695, 773, 894, 974, 1253, 1442, 3546, 3835
CseI	GACGC	3 cut(s)	381, 2672, 3140
CspAI	ACCGGT	1 cut(s)	697
DraI	TTTAAA	5 cut(s)	951, 1356, 1762, 3595, 3922
DrdI	GACNNNNNNGTC	1 cut(s)	80
DseDI	GACNNNNNNGTC	1 cut(s)	80
EaeI	YGGCCR	3 cut(s)	1519, 2031, 2162
Eam1104I	CTCTTC	5 cut(s)	162, 323, 806, 1011, 2499
EarI	CTCTTC	5 cut(s)	162, 323, 806, 1011, 2499
EciI	GGCGGA	2 cut(s)	360, 1568
Ecl136II	GAGCTC	2 cut(s)	28, 1423
Eco130I	CCWWGG	4 cut(s)	881, 1173, 1844, 2740
Eco147I	AGGCCT	3 cut(s)	430, 1679, 1929
Eco24I	GRGCYC	3 cut(s)	30, 1425, 3101
Eco31I	GGTCTC	1 cut(s)	3557
Eco32I	GATATC	1 cut(s)	1472
Eco47I	GGWCC	7 cut(s)	244, 695, 894, 1253, 1442, 3546, 3835
Eco53kI	GAGCTC	2 cut(s)	28, 1423
Eco57I	CTGAAG	2 cut(s)	787, 3872
Eco88I	CYCGRG	2 cut(s)	8, 609
EcoICRI	GAGCTC	2 cut(s)	28, 1423
EcoO109I	RGGNCCY	1 cut(s)	773
EcoRI	GAATTC	2 cut(s)	38, 1133
EcoRII	CCWGG	9 cut(s)	129, 496, 1679, 1721, 1857, 1929, 3408, 3436, 3532
EcoRV	GATATC	1 cut(s)	1472
EcoT14I	CCWWGG	4 cut(s)	881, 1173, 1844, 2740
EcoT22I	ATGCAT	2 cut(s)	909, 2398
EcoT38I	GRGCYC	3 cut(s)	30, 1425, 3101
ErhI	CCWWGG	4 cut(s)	881, 1173, 1844, 2740
Esp3I	CGTCTC	1 cut(s)	1241
FaqI	GGGAC	9 cut(s)	501, 708, 857, 1979, 2248, 2332, 2972, 3029, 3559
FauNDI	CATATG	1 cut(s)	3501
FbaI	TGATCA	2 cut(s)	589, 2943
FblI	GTMKAC	3 cut(s)	72, 2847, 3064
Fnu4HI	GCNGC	8 cut(s)	280, 309, 356, 648, 1740, 2054, 2774, 3231
FriOI	GRGCYC	3 cut(s)	30, 1425, 3101
Fsp4HI	GCNGC	8 cut(s)	280, 309, 356, 648, 1740, 2054, 2774, 3231
FspBI	CTAG	7 cut(s)	759, 882, 1547, 1845, 2426, 3249, 3680
GluI	GCNGC	8 cut(s)	280, 309, 356, 648, 1740, 2054, 2774, 3231
GsuI	CTGGAG	4 cut(s)	183, 519, 2112, 3044
HaeIII	GGCC	8 cut(s)	430, 775, 976, 1521, 1679, 1929, 2033, 2164
HapII	CCGG	3 cut(s)	396, 698, 2315
HgaI	GACGC	3 cut(s)	381, 2672, 3140
Hin1I	GRCGYC	1 cut(s)	3151
HincII	GTYRAC	3 cut(s)	73, 136, 844
HindII	GTYRAC	3 cut(s)	73, 136, 844
HindIII	AAGCTT	2 cut(s)	422, 2515
HpaI	GTTAAC	1 cut(s)	136
HpaII	CCGG	3 cut(s)	396, 698, 2315
Hpy166II	GTNNAC	7 cut(s)	73, 136, 844, 2285, 2848, 3065, 3458
Hpy8I	GTNNAC	7 cut(s)	73, 136, 844, 2285, 2848, 3065, 3458
Hpy99I	CGWCG	2 cut(s)	23, 326
HpyCH4III	ACNGT	6 cut(s)	725, 871, 1497, 2725, 3062, 3208
HpyCH4IV	ACGT	4 cut(s)	261, 1659, 1999, 3106
HpyF10VI	GCNNNNNNNGC	7 cut(s)	288, 2161, 2303, 2773, 2779, 3239, 3464
HpySE526I	ACGT	4 cut(s)	261, 1659, 1999, 3106
Hsp92I	GRCGYC	1 cut(s)	3151
Kpn2I	TCCGGA	2 cut(s)	395, 2314
Ksp22I	TGATCA	2 cut(s)	589, 2943
KspAI	GTTAAC	1 cut(s)	136
Lsp1109I	GCAGC	7 cut(s)	266, 342, 659, 1751, 2065, 2760, 3217
LweI	GCATC	6 cut(s)	1255, 1984, 2333, 2499, 2927, 3892
MaeI	CTAG	7 cut(s)	759, 882, 1547, 1845, 2426, 3249, 3680
MaeII	ACGT	4 cut(s)	261, 1659, 1999, 3106
MaeIII	GTNAC	3 cut(s)	557, 761, 2950
MfeI	CAATTG	3 cut(s)	2859, 2980, 3468
MflI	RGATCY	7 cut(s)	457, 1190, 1210, 2065, 3310, 3361, 3733
MhlI	GDGCHC	7 cut(s)	30, 730, 1425, 1708, 3101, 3265, 3460
MlsI	TGGCCA	3 cut(s)	1521, 2033, 2164
MluNI	TGGCCA	3 cut(s)	1521, 2033, 2164
MlyI	GAGTC	6 cut(s)	322, 554, 1148, 1314, 1877, 3057
MmeI	TCCRAC	5 cut(s)	809, 1032, 1131, 2028, 2670
Mox20I	TGGCCA	3 cut(s)	1521, 2033, 2164
Mph1103I	ATGCAT	2 cut(s)	909, 2398
MroI	TCCGGA	2 cut(s)	395, 2314
MscI	TGGCCA	3 cut(s)	1521, 2033, 2164
MslI	CAYNNNNRTG	3 cut(s)	1829, 1941, 2538
Msp20I	TGGCCA	3 cut(s)	1521, 2033, 2164
MspA1I	CMGCKG	1 cut(s)	2056
MspCI	CTTAAG	1 cut(s)	1583
MspI	CCGG	3 cut(s)	396, 698, 2315
MspR9I	CCNGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
MunI	CAATTG	3 cut(s)	2859, 2980, 3468
Mva1269I	GAATGC	2 cut(s)	2494, 3577
MvaI	CCWGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
MvnI	CGCG	1 cut(s)	332
MwoI	GCNNNNNNNGC	7 cut(s)	288, 2161, 2303, 2773, 2779, 3239, 3464
NcoI	CCATGG	1 cut(s)	2740
NdeI	CATATG	1 cut(s)	3501
NlaIV	GGNNCC	9 cut(s)	696, 774, 895, 1192, 2225, 2252, 3100, 3262, 3547
NmuCI	GTSAC	2 cut(s)	557, 2950
NsiI	ATGCAT	2 cut(s)	909, 2398
NspI	RCATGY	3 cut(s)	2396, 2870, 3353
NspV	TTCGAA	2 cut(s)	144, 2804
PaeI	GCATGC	1 cut(s)	2396
PagI	TCATGA	2 cut(s)	1180, 2536
PaqCI	CACCTGC	1 cut(s)	296
PceI	AGGCCT	3 cut(s)	430, 1679, 1929
PciI	ACATGT	1 cut(s)	3349
PcsI	WCGNNNNNNNCGW	2 cut(s)	15, 171
PctI	GAATGC	2 cut(s)	2494, 3577
PfeI	GAWTC	9 cut(s)	913, 1034, 1112, 1808, 1953, 2414, 2578, 2597, 2806
PfoI	TCCNGGA	1 cut(s)	3436
PinAI	ACCGGT	1 cut(s)	697
PkrI	GCNGC	8 cut(s)	281, 310, 357, 649, 1741, 2055, 2775, 3232
PleI	GAGTC	6 cut(s)	321, 554, 1148, 1313, 1876, 3056
PpsI	GAGTC	6 cut(s)	321, 554, 1148, 1313, 1876, 3056
Ppu21I	YACGTR	1 cut(s)	2000
PscI	ACATGT	1 cut(s)	3349
PsiI	TTATAA	2 cut(s)	2387, 2966
Psp124BI	GAGCTC	2 cut(s)	30, 1425
Psp6I	CCWGG	9 cut(s)	129, 496, 1679, 1721, 1857, 1929, 3408, 3436, 3532
PspGI	CCWGG	9 cut(s)	129, 496, 1679, 1721, 1857, 1929, 3408, 3436, 3532
PspN4I	GGNNCC	9 cut(s)	696, 774, 895, 1192, 2225, 2252, 3100, 3262, 3547
PspPI	GGNCC	9 cut(s)	244, 695, 773, 894, 974, 1253, 1442, 3546, 3835
PsrI	GAACNNNNNNTAC	2 cut(s)	3159, 3191
PstI	CTGCAG	4 cut(s)	1741, 2083, 2892, 3235
PstNI	CAGNNNCTG	1 cut(s)	3806
PsuI	RGATCY	7 cut(s)	457, 1190, 1210, 2065, 3310, 3361, 3733
PvuII	CAGCTG	1 cut(s)	2056
RseI	CAYNNNNRTG	3 cut(s)	1829, 1941, 2538
SacI	GAGCTC	2 cut(s)	30, 1425
SalI	GTCGAC	1 cut(s)	71
SatI	GCNGC	8 cut(s)	280, 309, 356, 648, 1740, 2054, 2774, 3231
Sau96I	GGNCC	9 cut(s)	244, 695, 773, 894, 974, 1253, 1442, 3546, 3835
SbfI	CCTGCAGG	1 cut(s)	2892
ScaI	AGTACT	2 cut(s)	981, 1838
SchI	GAGTC	6 cut(s)	322, 554, 1148, 1314, 1877, 3057
ScrFI	CCNGG	9 cut(s)	131, 498, 1681, 1723, 1859, 1931, 3410, 3438, 3534
SdaI	CCTGCAGG	1 cut(s)	2892
SduI	GDGCHC	7 cut(s)	30, 730, 1425, 1708, 3101, 3265, 3460
SfaNI	GCATC	6 cut(s)	1255, 1984, 2333, 2499, 2927, 3892
SfcI	CTRYAG	9 cut(s)	57, 1493, 1737, 2079, 2376, 2848, 2888, 3231, 3316
SfuI	TTCGAA	2 cut(s)	144, 2804
SinI	GGWCC	7 cut(s)	244, 695, 894, 1253, 1442, 3546, 3835
SmiMI	CAYNNNNRTG	3 cut(s)	1829, 1941, 2538
SmlI	CTYRAG	3 cut(s)	1103, 1583, 3325
SmoI	CTYRAG	3 cut(s)	1103, 1583, 3325
SpeI	ACTAGT	1 cut(s)	3679
SphI	GCATGC	1 cut(s)	2396
Sse8387I	CCTGCAGG	1 cut(s)	2892
SseBI	AGGCCT	3 cut(s)	430, 1679, 1929
SsiI	CCGC	8 cut(s)	308, 332, 345, 1579, 1612, 2139, 2335, 3077
SspI	AATATT	2 cut(s)	369, 2434
SspMI	CTAG	7 cut(s)	759, 882, 1547, 1845, 2426, 3249, 3680
SstI	GAGCTC	2 cut(s)	30, 1425
StuI	AGGCCT	3 cut(s)	430, 1679, 1929
StyD4I	CCNGG	9 cut(s)	129, 496, 1679, 1721, 1857, 1929, 3408, 3436, 3532
StyI	CCWWGG	4 cut(s)	881, 1173, 1844, 2740
TaaI	ACNGT	6 cut(s)	725, 871, 1497, 2725, 3062, 3208
TaiI	ACGT	4 cut(s)	264, 1662, 2002, 3109
TatI	WGTACW	6 cut(s)	64, 979, 1448, 1821, 1836, 2190
TauI	GCSGC	1 cut(s)	311
TfiI	GAWTC	9 cut(s)	913, 1034, 1112, 1808, 1953, 2414, 2578, 2597, 2806
TscAI	CASTG	7 cut(s)	684, 730, 754, 811, 2728, 3300, 3811
TseFI	GTSAC	2 cut(s)	557, 2950
TseI	GCWGC	7 cut(s)	279, 355, 647, 1739, 2053, 2773, 3230
Tsp45I	GTSAC	2 cut(s)	557, 2950
TspGWI	ACGGA	8 cut(s)	180, 731, 1223, 2346, 3139, 3301, 3761, 3827
TspRI	CASTG	7 cut(s)	684, 730, 754, 811, 2728, 3300, 3811
Vha464I	CTTAAG	1 cut(s)	1583
VneI	GTGCAC	1 cut(s)	3456
VpaK11BI	GGWCC	7 cut(s)	244, 695, 894, 1253, 1442, 3546, 3835
XapI	RAATTY	8 cut(s)	38, 598, 656, 862, 1133, 2309, 2835, 3879
XceI	RCATGY	3 cut(s)	2396, 2870, 3353
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2686
XmaJI	CCTAGG	2 cut(s)	881, 1844
XmiI	GTMKAC	3 cut(s)	72, 2847, 3064
XspI	CTAG	7 cut(s)	759, 882, 1547, 1845, 2426, 3249, 3680
ZrmI	AGTACT	2 cut(s)	981, 1838
Zsp2I	ATGCAT	2 cut(s)	909, 2398

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.