Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0010438

Physical Location & Seq

Genomic Coordinates Reverse (-)

27769860 .. 27789622

Gene Structure 19763 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00351540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3714 bp

ATGGGACATAGTTCGAAGAAGAAGAAGCGCGGAGGAGGAGGAGGAGGAGGCTGTGGGAAGAAGAAGAAGCCCTTGAAAGATCATGCCGGGGCTTACGATAATGACCTTCTTTCTGAAGAGATCACTGCTTTGTGTGCAATATTTCAAGATGACTGCAAAATTGCGTCCGGATCACAACCTCAATTCACCATCAAGCTTAGGCCTCACTCAAAGGATATGGGTTATGAGGATCTGGATGTCTCTGCACTTCTTTTAGTTAGGTGCTTACCAGGGTATCCTTACAAGTGTCCCAAGTTGCAGATAACTCCAGAGAAAGGATTATCGAAAAGTGACTCTGATAAGCTATTATCTCTTATCAATGATCAGGCAAATTCCAATGCCCGAGAAGGACGGGTAATGATCTTCAATTTGGTTGAGGCAGCTCAGGAATTTCTATCTGAAATTGTGCCAGTGGGTCAATCACAGGGACCAGTTATATGTTCAACTACGGATAACAGTGCTCAGTTGTTCCAAAATGACACTGCAATTTCTAGTAACAAGAAGGGGCCTTTTGTTTATGGTTTCATAGACCTCTTCAGTGGCTCTGGAAAGTCTTGGAGTTGGAGTTTTGGAGTTGATGAGACTAAAGGGATAAATTCTACTGTCCCATTGCCTAGGGTGGATGGGTCCAAACTCATGCATGAGATTCAGGAAAAGAAACATGATAAGCAAGCAGAACCTTTAAAATCACAAGATATAAAGCAAGGGCCAGTACTTTCCCCTAATGTCAAGTTAGAAACGCTTGAAGAAGAGAGTGAAGGCAGGGATTCAAGTAGATTTTTGTTGGAAGAAATGGACAGAAATGGTGGTGAAACTGAGAAAGAGAGTTCAGTTCCTGAGGAGGATTCAACAGAAGAAGATTGGGAATTCGGAAGTCAGCAGTCGGAATCGCTCTCTTTTACTTCCTTGGCTCATGAGCAAGGATCCCAAAATGTTAAAAGGGATCTAATAATGGTTCATCTGCTCCGTCTCGCCTGCACTAAAGGACCATTGGCTGATGCTTTGCCACAGATAACCACAGAACTGCAAAACATAGGGGTCTTATCAGAATGGGCAAGGGATTTAGCTTCAAAACCAATTTCACTTTTAAATCGAACATTTAATCATGCCTTCGGACAACAGATGGTTTCATCCCGTATCTCTCAATTCTGGGAGCTCACTTCTGATTTAGAAGGACCAAGTACATCTCTCCCAAGTTCTCGATATCTCAATGACTTTGAGGAGCTACAGTCTCTTGGTCATGGTGGCTTTGGCCATGTTGTGTTGTGCAAAAATAAGCTAGATGGAAGGCAGTATGCAGTAAAGAAAATCCGCCTCAAGGATAAAAGCCTGCCACTTGATGACCGCATATTGAGGGAAGTTGCTACACTTTCTCGTTTGCAACATCAACATGTTGTACGATACTATCAGGCCTGGTTTGAAACAGGAGGTGTTGGTGCTCATGGTGATACTACCTGGGGTTCAAGGACTGCAGCCAGCTCCATTTTTAGCTTTAAAGGTACAAGCTCAGCAGATGATTTTGGGAATGAGAATAAACTTGAATCAACATATTTGTACATTCAAATGGAGTACTGCCCTAGGACATTACGCCAGGTTTTTGAGTCGTATAGTCAATTTGACAAAGAATTAGCATGGCATTTGTTTCGTCAAATTGTTGAAGGCCTGGCACACATACACGGACAAGGAATCATCCATCGGGACTTAACGCCAAATAACATATTCTTTGATGCACGTAATGATATTAAAATTGGAGATTTTGGTTTGGCCAAGTTTTTGAAGTTGGAGCAGCTGGATCAAGATCCAAGTTTTCCTGCAGATACAACTGGAGTTTCTCTTGATGGTACTGGTCAAGTTGGTACATATTTTTACACCGCACCTGAAATTGAGCAAGGCTGGCCAAAGATTGATGAAAAGGCTGATATGTACAGCTTGGGAATTGTATTTTTCGAGCTATGGCACCCATTTGGGACTGCAATGGAACGGCACCTTGTCCTTTCCGACTTGAAACAGAAGGGTGAACTGCCTTCTGCTTGGGTTGCTGAATTTCCGGAACAAGCATCCTTGTTGCGGAGCTTAATGTCCCCAAGTCCGTCAGATCGCCCTTCTGCTACAGAACTTATAAAGCATGCATTTCCACCACGAATGGAATCTGAGTTGCTAGACAATATTCTACGAACAATGCAAACCTCAGAGGACAGAACTGTGTATGATAAAGTTTTGAATACTATATTTGATGAAGAGATGCTAAGCTTGAAAGACCAGCAACATCATGATGGTAGGTTGAGGTTGGCTGGTAGCGATACTTCTGCGATTCAATATGCAGATTTAGATTCGGAGGCTCGTGATTATGTTGTTGAGATCACAAGGGAGGTGTTCAGACAGCATTGTGCGAAGCACCTTGAAGTAATACCCATGCGTCTGTTGGATGATTGCCAACATTTTATGAGAAGGAACACTGTCAAACTTTTGACCCATGGAGGAGATATGCTTGAACTTTTGCATGAGCTGCGTTTGCCTTTCGTTAGTTGGGTGATTTCGAATCAGAAATCTTCATTCAAAAGATATGAAATTTCATGTGTCTACAGAAGACCAATTGGACATGCACCCCCAAGTCGCTACCTGCAGGGTGATTTTGACATTATTGGAGGTGCATCAGCATTGACAGAGGCAGAAGTGATCAAGGTGACAAGGGACATTGTAACCCGTTTTTTCCATTGGGAGTTCTGCGACATTCATCTAAACCACGGGGACTTACTGGAGGCAATTTGGTCTTGGGTAGGAGTCAAGGCAGACCATAGACAAAAAGTAGCGGAGCTTCTTTCTATGATGGGCTCCTTACGTCCTCAGTCTTCTGAACGGAAATCCAAATGGGTGGTAATAAGGCGTCAGCTTTTGCAGGAACTTAATTTACAAGAGGCTGTTGTGAATAGGTTGCAGACTGTTGGTTTAAGGTTCTGTGGAGCTGCAGATCAGGCAATTCCTAGACTTAGGGGAGCCCTGCCAAATGATAAACCAACACGGAAGGCACTAGATGAGTTGTCAGATCTCTGTAGCTACTTGAGAGCTTGGAGGATTGAAGAACATGTTTATATAGATCCACTTATCCCCCCAACTGAGAGTTATCATAGAGACTTGTTTTTCCAGGTATATCTGGTGAAGGATAGTAATCCTGGATCACTTACCGAAGGTGCACTACTTGCAATTGGGGGTCGGTATGACTATTTGCTTCATCATATGTGGAGTCTTGAACATAAATCTAATCCTCCTGGTTCTGTTGGGACCAGCCTTGCTTTAGAGACCATAATTCAGCATTCTCCTGTGGATTTTAAACCAATTAGAAATGAAGTTAGCAATAGTGTTCTTGTTTGTTCAAAAGGAGGAGGGGGTCTATTGGCAGAACGCATGGAACTAGTCAATGAGCTTTGGGAAGAAAACATTAAGGCTGAGTTTCTCCCAACACCAGATCCAAGCCTTACGGAGCAGTATGAATATGCCAACGAACATGATATTAAATGTCTTGTCATCATAACAGACACTGGTGTTTCTCAGAAAGGTTCTGTTAAGGTCCGTCATCTTGAACTGAAGAAGGAAAAGGAGGTTGAAAGGGAAATTCTCGTCAGGTTTCTGCTGGATGCAATGGCAATACAATTTAAAAATCCTTCCATTTGGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0014070 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034198 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0046677 GO:0050896 GO:0051716 GO:0060992 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928

KEGG Pathways

Metabolic & Signaling

ko04138 ko04140 ko04141 ko05160 ko05162 ko05164 ko05168 ko05169 map04138 map04140 map04141 map05160 map05162 map05164 map05168 map05169

Pfam Domains

Protein Families

HGTP_anticodon2 Pkinase RWD tRNA-synt_His

Protein Analysis

1237

Amino Acids

139.55

Weight (kDa)

5.7

Isoelectric Point (pI)

42.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWD	PF05773	36 - 145	1e-18	RWD domain
Pkinase	PF00069	419 - 724	8.5e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	529 - 721	4.3e-16	Protein tyrosine and serine/threonine kinase
tRNA-synt_His	PF13393	871 - 1113	2.7e-10	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	1133 - 1227	3.1e-09	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	1134 - 1223	2.3e-07	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59410 AT3G59410 AT3G59410 AT3G59410 AT3G59410
fragaria_vesca	FvH4_4g10800 FvH4_4g10800
malus_domestica	MD04G1040400.v1.1
prunus_persica	Prupe.1G194100_v2.0.a1 Prupe.1G194100_v2.0.a1
pyrus_communis	pycom04g03520
rosa_chinensis	RchiOBHm_Chr4g0406781
rosa_laevigata	RLG00000008759
rosa_multiflora	Rmu_sc0006408.1_g000006
rosa_roxburghii	Rroxscaffold_5G00351540
rosa_rugosa	Rorug04G0072900
rosa_samantha	Rh4AG140400 Rh4BG137400 Rh4CG147300 Rh4DG134400
rosa_wichuraiana	Rw4G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2159
Acc36I	ACCTGC	1 cut(s)	2667
AccB1I	GGYRCC	2 cut(s)	1995, 2022
AccI	GTMKAC	1 cut(s)	2619
AccII	CGCG	1 cut(s)	30
AccIII	TCCGGA	2 cut(s)	167, 2086
AciI	CCGC	6 cut(s)	30, 1351, 1384, 1911, 2107, 2849
AcoI	YGGCCR	3 cut(s)	1291, 1803, 1934
AcsI	RAATTY	7 cut(s)	370, 428, 634, 905, 2081, 2607, 3648
AcuI	CTGAAG	3 cut(s)	135, 559, 3641
AcyI	GRCGYC	1 cut(s)	2923
AfaI	GTAC	9 cut(s)	753, 1222, 1437, 1540, 1595, 1610, 1882, 1897, 1964
AfiI	CCNNNNNNNGG	3 cut(s)	314, 1357, 2106
AflIII	ACRYGT	2 cut(s)	1429, 3121
AhlI	ACTAGT	1 cut(s)	3448
AjnI	CCWGG	8 cut(s)	268, 1451, 1493, 1629, 1701, 3180, 3208, 3304
Alw21I	GWGCWC	4 cut(s)	502, 1197, 1480, 3232
Alw26I	GTCTC	6 cut(s)	244, 614, 1013, 1275, 3162, 3329
Alw44I	GTGCAC	1 cut(s)	3228
AlwNI	CAGNNNCTG	2 cut(s)	875, 3575
Ama87I	CYCGRG	1 cut(s)	381
Aor13HI	TCCGGA	2 cut(s)	167, 2086
AoxI	GGCC	8 cut(s)	200, 545, 746, 1291, 1449, 1699, 1803, 1934
ApaLI	GTGCAC	1 cut(s)	3228
ApeKI	GCWGC	5 cut(s)	419, 1511, 1825, 2545, 3002
ApoI	RAATTY	7 cut(s)	370, 428, 634, 905, 2081, 2607, 3648
AspA2I	CCTAGG	2 cut(s)	653, 1616
AspLEI	GCGC	1 cut(s)	30
AspS9I	GGNCC	8 cut(s)	467, 545, 666, 746, 1025, 1214, 3318, 3604
AsuC2I	CCSGG	1 cut(s)	88
AsuHPI	GGTGA	8 cut(s)	178, 860, 1496, 2066, 2581, 2678, 2734, 3205
AsuII	TTCGAA	2 cut(s)	14, 2576
AvaI	CYCGRG	1 cut(s)	381
AvaII	GGWCC	6 cut(s)	467, 666, 1025, 1214, 3318, 3604
AvrII	CCTAGG	2 cut(s)	653, 1616
AxyI	CCTNAGG	1 cut(s)	876
BaeGI	GKGCMC	1 cut(s)	3232
BaeI	ACNNNNGTAYC	2 cut(s)	257, 290
BalI	TGGCCA	3 cut(s)	1293, 1805, 1936
BamHI	GGATCC	1 cut(s)	962
BanI	GGYRCC	2 cut(s)	1995, 2022
BanII	GRGCYC	3 cut(s)	1197, 2873, 3037
BarI	GAAGNNNNNNTAC	6 cut(s)	2326, 2358, 2838, 2870, 3216, 3248
BauI	CACGAG	1 cut(s)	2379
BbsI	GAAGAC	2 cut(s)	2632, 2880
Bbv12I	GWGCWC	4 cut(s)	502, 1197, 1480, 3232
BbvI	GCAGC	5 cut(s)	431, 1523, 1837, 2532, 2989
BccI	CCATC	8 cut(s)	197, 656, 1156, 1316, 1740, 1871, 2306, 2860
BceAI	ACGGC	1 cut(s)	2036
BcgI	CGANNNNNNTGC	4 cut(s)	1664, 1698, 2327, 2361
BciT130I	CCWGG	8 cut(s)	270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
BciVI	GTATCC	1 cut(s)	285
BclI	TGATCA	2 cut(s)	361, 2715
BcnI	CCSGG	1 cut(s)	88
BcoDI	GTCTC	6 cut(s)	244, 614, 1013, 1275, 3162, 3329
BcuI	ACTAGT	1 cut(s)	3448
BfaI	CTAG	8 cut(s)	531, 654, 1319, 1617, 2198, 3021, 3068, 3449
BfmI	CTRYAG	8 cut(s)	1265, 1509, 1851, 2148, 2620, 2660, 3003, 3088
BfuAI	ACCTGC	1 cut(s)	2667
BfuI	GTATCC	1 cut(s)	285
BglII	AGATCT	1 cut(s)	3082
BisI	GCNGC	5 cut(s)	420, 1512, 1826, 2546, 3003
BlnI	CCTAGG	2 cut(s)	653, 1616
BlpI	GCTNAGC	2 cut(s)	1546, 2285
BlsI	GCNGC	5 cut(s)	421, 1513, 1827, 2547, 3004
BmcAI	AGTACT	2 cut(s)	753, 1610
Bme1390I	CCNGG	9 cut(s)	88, 270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
Bme18I	GGWCC	6 cut(s)	467, 666, 1025, 1214, 3318, 3604
BmeT110I	CYCGRG	1 cut(s)	381
BmgT120I	GGNCC	8 cut(s)	467, 545, 666, 746, 1025, 1214, 3318, 3604
BmiI	GGNNCC	9 cut(s)	468, 546, 667, 964, 1997, 2024, 2872, 3034, 3319
BmrFI	CCNGG	9 cut(s)	88, 270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
BmsI	GCATC	6 cut(s)	1027, 1756, 2105, 2271, 2699, 3661
BpiI	GAAGAC	2 cut(s)	2632, 2880
BpmI	CTGGAG	3 cut(s)	291, 1884, 2816
Bpu10I	CCTNAGC	2 cut(s)	197, 423
Bpu1102I	GCTNAGC	2 cut(s)	1546, 2285
Bpu14I	TTCGAA	2 cut(s)	14, 2576
BpuEI	CTTGAG	2 cut(s)	1340, 3118
BpuMI	CCSGG	1 cut(s)	88
BsaAI	YACGTR	1 cut(s)	1772
BsaBI	GATNNNNATC	2 cut(s)	1836, 3204
BsaHI	GRCGYC	1 cut(s)	2923
BsaI	GGTCTC	1 cut(s)	3329
BsaJI	CCNNGG	8 cut(s)	87, 269, 653, 945, 1494, 1616, 2512, 2782
BsaWI	WCCGGW	2 cut(s)	167, 2086
BsaXI	ACNNNNNCTCC	4 cut(s)	589, 619, 1253, 1283
Bsc4I	CCNNNNNNNGG	3 cut(s)	314, 1357, 2106
Bse1I	ACTGG	7 cut(s)	449, 470, 749, 1867, 1888, 2799, 3580
Bse21I	CCTNAGG	1 cut(s)	876
Bse3DI	GCAATG	3 cut(s)	647, 2019, 3681
Bse8I	GATNNNNATC	2 cut(s)	1836, 3204
BseAI	TCCGGA	2 cut(s)	167, 2086
BseBI	CCWGG	8 cut(s)	270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
BseDI	CCNNGG	8 cut(s)	87, 269, 653, 945, 1494, 1616, 2512, 2782
BseGI	GGATG	7 cut(s)	241, 667, 1169, 1728, 2096, 2470, 3676
BseJI	GATNNNNATC	2 cut(s)	1836, 3204
BseLI	CCNNNNNNNGG	3 cut(s)	314, 1357, 2106
BseMI	GCAATG	3 cut(s)	647, 2019, 3681
BseNI	ACTGG	7 cut(s)	449, 470, 749, 1867, 1888, 2799, 3580
BseRI	GAGGAG	9 cut(s)	48, 51, 54, 57, 60, 893, 1274, 2532, 3432
BseSI	GKGCMC	1 cut(s)	3232
BseXI	GCAGC	5 cut(s)	431, 1523, 1837, 2532, 2989
BsgI	GTGCAG	2 cut(s)	228, 1000
Bsh1236I	CGCG	1 cut(s)	30
BshFI	GGCC	8 cut(s)	202, 547, 748, 1293, 1451, 1701, 1805, 1936
BshNI	GGYRCC	2 cut(s)	1995, 2022
BsiHKAI	GWGCWC	4 cut(s)	502, 1197, 1480, 3232
BsiHKCI	CYCGRG	1 cut(s)	381
BsiSI	CCGG	3 cut(s)	87, 168, 2087
BslI	CCNNNNNNNGG	3 cut(s)	314, 1357, 2106
BsmAI	GTCTC	6 cut(s)	244, 614, 1013, 1275, 3162, 3329
BsmBI	CGTCTC	1 cut(s)	1013
BsmI	GAATGC	1 cut(s)	3349
BsnI	GGCC	8 cut(s)	202, 547, 748, 1293, 1451, 1701, 1805, 1936
Bso31I	GGTCTC	1 cut(s)	3329
BsoBI	CYCGRG	1 cut(s)	381
Bsp119I	TTCGAA	2 cut(s)	14, 2576
Bsp1286I	GDGCHC	6 cut(s)	502, 1197, 1480, 2873, 3037, 3232
Bsp13I	TCCGGA	2 cut(s)	167, 2086
Bsp1407I	TGTACA	2 cut(s)	1593, 1962
Bsp1720I	GCTNAGC	2 cut(s)	1546, 2285
Bsp19I	CCATGG	1 cut(s)	2512
BspACI	CCGC	6 cut(s)	30, 1351, 1384, 1911, 2107, 2849
BspANI	GGCC	8 cut(s)	202, 547, 748, 1293, 1451, 1701, 1805, 1936
BspEI	TCCGGA	2 cut(s)	167, 2086
BspFNI	CGCG	1 cut(s)	30
BspHI	TCATGA	2 cut(s)	952, 2308
BspLI	GGNNCC	9 cut(s)	468, 546, 667, 964, 1997, 2024, 2872, 3034, 3319
BspMAI	CTGCAG	4 cut(s)	1513, 1855, 2664, 3007
BspMI	ACCTGC	1 cut(s)	2667
BspT104I	TTCGAA	2 cut(s)	14, 2576
BspT107I	GGYRCC	2 cut(s)	1995, 2022
BspTNI	GGTCTC	1 cut(s)	3329
BsrDI	GCAATG	3 cut(s)	647, 2019, 3681
BsrGI	TGTACA	2 cut(s)	1593, 1962
BsrI	ACTGG	7 cut(s)	449, 470, 749, 1867, 1888, 2799, 3580
BssECI	CCNNGG	8 cut(s)	87, 269, 653, 945, 1494, 1616, 2512, 2782
BssNI	GRCGYC	1 cut(s)	2923
BssSI	CACGAG	1 cut(s)	2379
BssT1I	CCWWGG	4 cut(s)	653, 945, 1616, 2512
Bst2BI	CACGAG	1 cut(s)	2379
Bst2UI	CCWGG	8 cut(s)	270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
Bst4CI	ACNGT	6 cut(s)	497, 643, 1269, 2242, 2497, 2980
Bst6I	CTCTTC	4 cut(s)	111, 578, 783, 2271
BstACI	GRCGYC	1 cut(s)	2923
BstAPI	GCANNNNNTGC	2 cut(s)	2545, 3236
BstAUI	TGTACA	2 cut(s)	1593, 1962
BstBAI	YACGTR	1 cut(s)	1772
BstBI	TTCGAA	2 cut(s)	14, 2576
BstC8I	GCNNGC	6 cut(s)	711, 1015, 1370, 1516, 1934, 2166
BstDSI	CCRYGG	2 cut(s)	2512, 2782
BstF5I	GGATG	7 cut(s)	241, 667, 1169, 1728, 2096, 2470, 3676
BstFNI	CGCG	1 cut(s)	30
BstHHI	GCGC	1 cut(s)	30
BstMAI	GTCTC	6 cut(s)	244, 614, 1013, 1275, 3162, 3329
BstMWI	GCNNNNNNNGC	7 cut(s)	134, 1933, 2075, 2545, 2551, 3011, 3236
BstNI	CCWGG	8 cut(s)	270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
BstNSI	RCATGY	4 cut(s)	1433, 2168, 2642, 3125
BstSCI	CCNGG	9 cut(s)	86, 268, 1451, 1493, 1629, 1701, 3180, 3208, 3304
BstSFI	CTRYAG	8 cut(s)	1265, 1509, 1851, 2148, 2620, 2660, 3003, 3088
BstSLI	GKGCMC	1 cut(s)	3232
BstUI	CGCG	1 cut(s)	30
BstV1I	GCAGC	5 cut(s)	431, 1523, 1837, 2532, 2989
BstV2I	GAAGAC	2 cut(s)	2632, 2880
BstX2I	RGATCY	7 cut(s)	229, 962, 982, 1837, 3082, 3133, 3502
BstXI	CCANNNNNNTGG	1 cut(s)	2911
BstYI	RGATCY	7 cut(s)	229, 962, 982, 1837, 3082, 3133, 3502
Bsu36I	CCTNAGG	1 cut(s)	876
BsuI	GTATCC	1 cut(s)	285
BsuRI	GGCC	8 cut(s)	202, 547, 748, 1293, 1451, 1701, 1805, 1936
BtgI	CCRYGG	2 cut(s)	2512, 2782
BtsCI	GGATG	7 cut(s)	241, 667, 1169, 1728, 2096, 2470, 3676
BtsI	GCAGTG	2 cut(s)	123, 519
BtsIMutI	CAGTG	7 cut(s)	123, 456, 502, 519, 583, 2493, 3573
BveI	ACCTGC	1 cut(s)	2667
Cac8I	GCNNGC	6 cut(s)	711, 1015, 1370, 1516, 1934, 2166
CaiI	CAGNNNCTG	2 cut(s)	875, 3575
CciI	TCATGA	2 cut(s)	952, 2308
CfoI	GCGC	1 cut(s)	30
Cfr13I	GGNCC	8 cut(s)	467, 545, 666, 746, 1025, 1214, 3318, 3604
CseI	GACGC	3 cut(s)	153, 2444, 2912
Csp6I	GTAC	9 cut(s)	752, 1221, 1436, 1539, 1594, 1609, 1881, 1896, 1963
CviQI	GTAC	9 cut(s)	752, 1221, 1436, 1539, 1594, 1609, 1881, 1896, 1963
DraI	TTTAAA	5 cut(s)	723, 1128, 1534, 3367, 3691
EaeI	YGGCCR	3 cut(s)	1291, 1803, 1934
Eam1104I	CTCTTC	4 cut(s)	111, 578, 783, 2271
EarI	CTCTTC	4 cut(s)	111, 578, 783, 2271
EciI	GGCGGA	1 cut(s)	1340
Ecl136II	GAGCTC	1 cut(s)	1195
Eco130I	CCWWGG	4 cut(s)	653, 945, 1616, 2512
Eco147I	AGGCCT	3 cut(s)	202, 1451, 1701
Eco24I	GRGCYC	3 cut(s)	1197, 2873, 3037
Eco31I	GGTCTC	1 cut(s)	3329
Eco32I	GATATC	1 cut(s)	1244
Eco47I	GGWCC	6 cut(s)	467, 666, 1025, 1214, 3318, 3604
Eco53kI	GAGCTC	1 cut(s)	1195
Eco57I	CTGAAG	3 cut(s)	135, 559, 3641
Eco81I	CCTNAGG	1 cut(s)	876
Eco88I	CYCGRG	1 cut(s)	381
EcoICRI	GAGCTC	1 cut(s)	1195
EcoO109I	RGGNCCY	1 cut(s)	545
EcoRI	GAATTC	1 cut(s)	905
EcoRII	CCWGG	8 cut(s)	268, 1451, 1493, 1629, 1701, 3180, 3208, 3304
EcoRV	GATATC	1 cut(s)	1244
EcoT14I	CCWWGG	4 cut(s)	653, 945, 1616, 2512
EcoT22I	ATGCAT	2 cut(s)	681, 2170
EcoT38I	GRGCYC	3 cut(s)	1197, 2873, 3037
ErhI	CCWWGG	4 cut(s)	653, 945, 1616, 2512
Esp3I	CGTCTC	1 cut(s)	1013
FalI	AAGNNNNNCTT	2 cut(s)	56, 88
FauNDI	CATATG	1 cut(s)	3273
FbaI	TGATCA	2 cut(s)	361, 2715
FblI	GTMKAC	1 cut(s)	2619
Fnu4HI	GCNGC	5 cut(s)	420, 1512, 1826, 2546, 3003
FokI	GGATG	7 cut(s)	248, 674, 1156, 1715, 2083, 2477, 3683
FriOI	GRGCYC	3 cut(s)	1197, 2873, 3037
Fsp4HI	GCNGC	5 cut(s)	420, 1512, 1826, 2546, 3003
FspBI	CTAG	8 cut(s)	531, 654, 1319, 1617, 2198, 3021, 3068, 3449
GlaI	GCGC	1 cut(s)	29
GluI	GCNGC	5 cut(s)	420, 1512, 1826, 2546, 3003
GsuI	CTGGAG	3 cut(s)	291, 1884, 2816
HaeIII	GGCC	8 cut(s)	202, 547, 748, 1293, 1451, 1701, 1805, 1936
HapII	CCGG	3 cut(s)	87, 168, 2087
HgaI	GACGC	3 cut(s)	153, 2444, 2912
HhaI	GCGC	1 cut(s)	30
Hin1I	GRCGYC	1 cut(s)	2923
Hin6I	GCGC	1 cut(s)	28
HinP1I	GCGC	1 cut(s)	28
HindIII	AAGCTT	2 cut(s)	194, 2287
HpaII	CCGG	3 cut(s)	87, 168, 2087
HphI	GGTGA	8 cut(s)	178, 860, 1496, 2066, 2581, 2678, 2734, 3205
Hpy166II	GTNNAC	3 cut(s)	2057, 2620, 3230
Hpy8I	GTNNAC	3 cut(s)	2057, 2620, 3230
HpyCH4III	ACNGT	6 cut(s)	497, 643, 1269, 2242, 2497, 2980
HpyCH4IV	ACGT	2 cut(s)	1771, 2878
HpyF10VI	GCNNNNNNNGC	7 cut(s)	134, 1933, 2075, 2545, 2551, 3011, 3236
HpySE526I	ACGT	2 cut(s)	1771, 2878
Hsp92I	GRCGYC	1 cut(s)	2923
HspAI	GCGC	1 cut(s)	28
Kpn2I	TCCGGA	2 cut(s)	167, 2086
Ksp22I	TGATCA	2 cut(s)	361, 2715
Lsp1109I	GCAGC	5 cut(s)	431, 1523, 1837, 2532, 2989
LweI	GCATC	6 cut(s)	1027, 1756, 2105, 2271, 2699, 3661
MaeI	CTAG	8 cut(s)	531, 654, 1319, 1617, 2198, 3021, 3068, 3449
MaeII	ACGT	2 cut(s)	1771, 2878
MaeIII	GTNAC	4 cut(s)	329, 533, 2722, 2737
MfeI	CAATTG	2 cut(s)	2631, 3240
MflI	RGATCY	7 cut(s)	229, 962, 982, 1837, 3082, 3133, 3502
MhlI	GDGCHC	6 cut(s)	502, 1197, 1480, 2873, 3037, 3232
MlsI	TGGCCA	3 cut(s)	1293, 1805, 1936
MluNI	TGGCCA	3 cut(s)	1293, 1805, 1936
MlyI	GAGTC	4 cut(s)	326, 1649, 2829, 3289
MmeI	TCCRAC	6 cut(s)	581, 804, 903, 1800, 2061, 2442
Mox20I	TGGCCA	3 cut(s)	1293, 1805, 1936
Mph1103I	ATGCAT	2 cut(s)	681, 2170
MroI	TCCGGA	2 cut(s)	167, 2086
MscI	TGGCCA	3 cut(s)	1293, 1805, 1936
MslI	CAYNNNNRTG	3 cut(s)	1428, 1601, 2310
Msp20I	TGGCCA	3 cut(s)	1293, 1805, 1936
MspA1I	CMGCKG	1 cut(s)	1828
MspI	CCGG	3 cut(s)	87, 168, 2087
MspR9I	CCNGG	9 cut(s)	88, 270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
MunI	CAATTG	2 cut(s)	2631, 3240
Mva1269I	GAATGC	1 cut(s)	3349
MvaI	CCWGG	8 cut(s)	270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
MvnI	CGCG	1 cut(s)	30
MwoI	GCNNNNNNNGC	7 cut(s)	134, 1933, 2075, 2545, 2551, 3011, 3236
NciI	CCSGG	1 cut(s)	88
NcoI	CCATGG	1 cut(s)	2512
NdeI	CATATG	1 cut(s)	3273
NlaIV	GGNNCC	9 cut(s)	468, 546, 667, 964, 1997, 2024, 2872, 3034, 3319
NmuCI	GTSAC	2 cut(s)	329, 2722
NsiI	ATGCAT	2 cut(s)	681, 2170
NspI	RCATGY	4 cut(s)	1433, 2168, 2642, 3125
NspV	TTCGAA	2 cut(s)	14, 2576
PaeI	GCATGC	1 cut(s)	2168
PagI	TCATGA	2 cut(s)	952, 2308
PceI	AGGCCT	3 cut(s)	202, 1451, 1701
PciI	ACATGT	2 cut(s)	1429, 3121
PctI	GAATGC	1 cut(s)	3349
PfoI	TCCNGGA	1 cut(s)	3208
PkrI	GCNGC	5 cut(s)	421, 1513, 1827, 2547, 3004
PleI	GAGTC	4 cut(s)	326, 1648, 2828, 3288
PpsI	GAGTC	4 cut(s)	326, 1648, 2828, 3288
Ppu21I	YACGTR	1 cut(s)	1772
PscI	ACATGT	2 cut(s)	1429, 3121
PsiI	TTATAA	1 cut(s)	2159
Psp124BI	GAGCTC	1 cut(s)	1197
Psp6I	CCWGG	8 cut(s)	268, 1451, 1493, 1629, 1701, 3180, 3208, 3304
PspGI	CCWGG	8 cut(s)	268, 1451, 1493, 1629, 1701, 3180, 3208, 3304
PspN4I	GGNNCC	9 cut(s)	468, 546, 667, 964, 1997, 2024, 2872, 3034, 3319
PspPI	GGNCC	8 cut(s)	467, 545, 666, 746, 1025, 1214, 3318, 3604
PsrI	GAACNNNNNNTAC	2 cut(s)	2931, 2963
PstI	CTGCAG	4 cut(s)	1513, 1855, 2664, 3007
PstNI	CAGNNNCTG	2 cut(s)	875, 3575
PsuI	RGATCY	7 cut(s)	229, 962, 982, 1837, 3082, 3133, 3502
PvuII	CAGCTG	1 cut(s)	1828
RsaI	GTAC	9 cut(s)	753, 1222, 1437, 1540, 1595, 1610, 1882, 1897, 1964
RsaNI	GTAC	9 cut(s)	752, 1221, 1436, 1539, 1594, 1609, 1881, 1896, 1963
RseI	CAYNNNNRTG	3 cut(s)	1428, 1601, 2310
SacI	GAGCTC	1 cut(s)	1197
SatI	GCNGC	5 cut(s)	420, 1512, 1826, 2546, 3003
Sau96I	GGNCC	8 cut(s)	467, 545, 666, 746, 1025, 1214, 3318, 3604
SbfI	CCTGCAGG	1 cut(s)	2664
ScaI	AGTACT	2 cut(s)	753, 1610
SchI	GAGTC	4 cut(s)	326, 1649, 2829, 3289
ScrFI	CCNGG	9 cut(s)	88, 270, 1453, 1495, 1631, 1703, 3182, 3210, 3306
SdaI	CCTGCAGG	1 cut(s)	2664
SduI	GDGCHC	6 cut(s)	502, 1197, 1480, 2873, 3037, 3232
SfaNI	GCATC	6 cut(s)	1027, 1756, 2105, 2271, 2699, 3661
SfcI	CTRYAG	8 cut(s)	1265, 1509, 1851, 2148, 2620, 2660, 3003, 3088
SfuI	TTCGAA	2 cut(s)	14, 2576
SinI	GGWCC	6 cut(s)	467, 666, 1025, 1214, 3318, 3604
SmiMI	CAYNNNNRTG	3 cut(s)	1428, 1601, 2310
SmlI	CTYRAG	2 cut(s)	1355, 3097
SmoI	CTYRAG	2 cut(s)	1355, 3097
SpeI	ACTAGT	1 cut(s)	3448
SphI	GCATGC	1 cut(s)	2168
Sse8387I	CCTGCAGG	1 cut(s)	2664
SseBI	AGGCCT	3 cut(s)	202, 1451, 1701
SsiI	CCGC	6 cut(s)	30, 1351, 1384, 1911, 2107, 2849
SspI	AATATT	2 cut(s)	141, 2206
SspMI	CTAG	8 cut(s)	531, 654, 1319, 1617, 2198, 3021, 3068, 3449
SstI	GAGCTC	1 cut(s)	1197
StuI	AGGCCT	3 cut(s)	202, 1451, 1701
StyD4I	CCNGG	9 cut(s)	86, 268, 1451, 1493, 1629, 1701, 3180, 3208, 3304
StyI	CCWWGG	4 cut(s)	653, 945, 1616, 2512
TaaI	ACNGT	6 cut(s)	497, 643, 1269, 2242, 2497, 2980
TaiI	ACGT	2 cut(s)	1774, 2881
TaqI	TCGA	6 cut(s)	14, 323, 1132, 1240, 1986, 2576
TatI	WGTACW	5 cut(s)	751, 1220, 1593, 1608, 1962
TscAI	CASTG	7 cut(s)	130, 456, 502, 526, 583, 2500, 3580
TseFI	GTSAC	2 cut(s)	329, 2722
TseI	GCWGC	5 cut(s)	419, 1511, 1825, 2545, 3002
Tsp45I	GTSAC	2 cut(s)	329, 2722
TspGWI	ACGGA	8 cut(s)	503, 995, 1731, 2118, 2911, 3073, 3530, 3596
TspRI	CASTG	7 cut(s)	130, 456, 502, 526, 583, 2500, 3580
VneI	GTGCAC	1 cut(s)	3228
VpaK11BI	GGWCC	6 cut(s)	467, 666, 1025, 1214, 3318, 3604
XapI	RAATTY	7 cut(s)	370, 428, 634, 905, 2081, 2607, 3648
XceI	RCATGY	4 cut(s)	1433, 2168, 2642, 3125
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2458
XmaJI	CCTAGG	2 cut(s)	653, 1616
XmiI	GTMKAC	1 cut(s)	2619
XspI	CTAG	8 cut(s)	531, 654, 1319, 1617, 2198, 3021, 3068, 3449
ZrmI	AGTACT	2 cut(s)	753, 1610
Zsp2I	ATGCAT	2 cut(s)	681, 2170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.