Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0010438

Physical Location & Seq

Genomic Coordinates Reverse (-)

27001593 .. 27025885

Gene Structure 24293 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G011440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3708 bp

ATGGGACATAGTTCGAAGAAGAAGAAGCGCGGAGGAGGAGGAGGAGGCAGTGGGAAGAAGAAGAAGCCCTTGAAAGATCATGCCGGGGCTTACGATAATGACCTTCTTTCTGAAGAGATCACTGCTTTGTGTGCAATATTTCAAGATGACTGCAAAATTGCGTCCGGAGCACAACCTCAAATCACCATCAAGCTTAGGCCTCACTCAAAGGATATGGGTTATGAGGATCTGGATGTCTCTGCACTTCTTTTAGTTAGGTGCTTACCAGGGTATCCTTACAAGTGTCCCAAGTTGCAGATAACTCCCGAGAAAGGATTATCAAAAAGTGACTCTGATAAGCTATTATCTCTTATCAATGATCAGGCAAATTCCAATGCCCGAGAAGGACGGGTAATGATCTTCAATTTGGTCGAGGCAGCTCAGGAATTTCTATCTGAAATTGTGCCAGTGGGTCAATCACAGGGACCAGTAATATGTTCAACTATGGATAACAGTGCTCAGTTGTTTCAAAATGACACTGCAATTTCTAGTAACAAGAAGGGGCCTTTTGTTTATGGTTTCATAGACCTCTTCAGTGGCTCTGGAAAGTCTTGGAGTTGGAGTTTTGGAGTTGACGAGACTAAAGGGATAAATTCTACTGTCCCATTGCCTAGGGTGGATGGGTCCAAACTCATGCATGAGATTCAGGAAAAGAAACATGATAAGCAAGCAGAACCTTTAAAATCACAAGATATAAAGCAAGGGCCAATACTTTCCCCTAATGTCAAGTTAGAAACGCTTGAAGAAGAGAGTGAAGGCAGGGATTCAAGTAGATTTTTGTTGGAAGAAATGGACAGAAATGGTGGTGAAACTGAGAAAGAGAGTTCAGTTCCTGAGGAGGATTCAACAGAAGATGATTGGGAATTCGGAAGTCAGCAGTCGGACTCGCTCTCTTTTACTTCCTTGGCTCATGAGCAAGGATCCCAAAATGTTAAAAGGGATCTAATAATGGTTCATCTGCTCCGTCTCGCCTGCACTAAAGGACCATTGGCTGATGCTTTGCCACAGATAACCACAGAACTGCAAAACATAGGGGTCTTATCAGAATGGGCAAGGGATTTAGCTTCAAAACCAGTTTCACTTTTAAATCGAACATTTAATCATGCCTTCGGACAACAGATGGTTTCATCCCGTATCTCTCAATTCTGGGAGCTCACTTCTGATTTAGAAGGACCAAGTACATCTCTCCCAAGTTCTCGATATCTCAATGACTTTGAGGAGCTACAGTCTCTTGGTCATGGTGGCTTTGGCCATGTTGTGTTGTGCAAAAATAAGCTAGATGGAAGGCAGTATGCAGTAAAGAAAATCCGCCTCAAGGATAAAAGCCTGCCACTAGATGACCGCATATTGAGGGAAGTCGCTACACTTTCTCGTTTGCAACATCAACACGTTGTACGATACTATCAGGCCTGGTTTGAAACAGGAGGTGTTGGTGCTCATGGTGATACTACCTGGGGTTCAAGGACTGCAGCCAGCTCCACTTTTAGCTTTAAAGGTACAAGTTCGGCAGATGATCTTGGGAATGAGAATAAACTTGAATCAACATATTTGTACATTCAAATGGAGTACTGCCCTAGGACATTACGCCAGGTTTTTGAGTCGTATAGTCAATTTGACAAAGAATTAGCATGGCATTTGTTTCGTCAAATTGTTGAAGGCCTGGCACACATACATGGACAAGGAATCATCCATCGGGACTTAACGCCAAATAACATATTCTTTGATGCACGTAATGATATTAAAATTGGAGATTTTGGTTTGGCCAAGTTTTTGAAGTTGGAGCAGCTGGATCAAGATCCAAGTTTTCCTGCAGATACAACTGGAGTTTCTCTCGATGGTACTGGTCAAGTTGGTACATATTTTTACACTGCACCTGAAATTGAGCAAGGCTGGCCAAAGATTGATGAAAAGGCTGATATGTACAGCTTGGGAATTGTATTTTTCGAGCTATGGCACCCATTTGGGACTGCAATGGAATGGCACCTTGTCCTATCCGACTTGAAACTGAAGGGTGAACTGCCTTCTGCTTGGGTTGCTGAATTTCCGGAACAAGCATCCTTGTTGCGGAGCTTAATGTCCCCAAGTCCATCAGATCGCCCTTCTGCTACAGAACTTATAAAGCATGCATTTCCACCACGAATGGAATCTGAGTTGCTAGACAATATTCTACGAACAATGCAAACCTCAGAGGACAGAAGTGTGTATGATAAAGTTCTGAATGCTATATTTGATGAAGAGATGCTAAGCTTGAAAGACCAGCAACATCATGATGGTAGGTTGAGGTTGGCTGGTAGCGATACTTCTGCGATTCAGTATGCAGATTTAGATTCGGAGGCTCGTGATTATGTTGTTGAGATCACAAGGGAGGTGTTCAGACAACATTGTGCGAAGCACCTTGAAGTAATACCCATGCGTCTGTTGGATGATTGCCAACATTTTATGAGGAACACTGTCAAACTTTTGACCCATGGAGGAGATATGCTTGAACTTTTGCATGAGCTGCGTTTGCCTTTTGTTAGTTGGGTGATTTCGAATCAGAAATCTTCATTCAAAAGATATGAAATTTCATGTGTCTACAGAAGACCAATTGGACATGCACCCCCAAGTCGCTACCTGCAGGGTGATTTCGACATTATTGGAGGTGCATCAGCATTGACAGAGGCAGAAGTGATCAAGGTGACAAGGGACATTGTAACCCGTTTTTTCAATTGGGAGTTCTGCGACATTCATCTAAACCACGGGGACTTACTGGAGGCAATTTGGTCTTGGGTAGGAGTCAAGGCAGACCATAGACAAAAAGTAGCGGAGCTTCTTTCTATGATGGGCTCCTTACGTCCTCAGTCTTCTGAACGGAAATCCAAATGGGTGGTAATAAGGCGTCAGCTTTTGCAGGAACTTAATTTACAAGCGGCTGTTGTGAATAGGTTGCAGACTGTTGGTTTAAGGTTCTGTGGAGCTGCAGATCAGGCACTTCCTAGACTTAGGGGAGCCCTGCCAAACGATAAACCAACACGGAAGGCACTTGATGAGTTGTCAGATCTCTGTAGCTACTTGAGAGCTTGGAGGATTGAAGAACATGTTTATATAGATCCACTTATCCCCCCAACTGAGAGTTATCATAGAGACTTGTTTTTCCAGGTATATTTGGTGAAGGATAGTAATCCTGGATCACTTACCGAAGGTGCACTACTTGCAATTGGGGGTCGGTATGACTATTTGCTTCATCATATGTGGGGTCTTGAACATAAATCTAATCCTCCTGGTTCTGTTGGGACCAGCCTTGCTTTAGAGACCATAATTCAGCATTCTCCTGTGGATTTTAAACCAATTAGAAATGAAGTTAGCAATAGTGTTCTTGTTTGTTCAAAAGGAGGAGGGGGTCTATTGGCAGAACGCATGGAACTAGTCAATGAGCTTTGGGAAGAAAACATTAAGGCTGAGTTTCTCCCAACACCAGATCCAAGCCTTACGGAGCAGTATGAATATGCCAACGAACATGATATCAAATGTCTTGTCATCATAACAGACACTGGTGTTTCTCAGAAAGGTTCTGTTATGGTCCGTCATCTTGAACTGAAGAAGGAGAAGGAGGTTGAAAGGGAAATTCTTGTCAGGTTTCTGCTGGATGCAATGGCAATACAATTTAAAAATCCTTCCATTTGGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0014070 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034198 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0046677 GO:0050896 GO:0051716 GO:0060992 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928

KEGG Pathways

Metabolic & Signaling

ko04138 ko04140 ko04141 ko05160 ko05162 ko05164 ko05168 ko05169 map04138 map04140 map04141 map05160 map05162 map05164 map05168 map05169

Pfam Domains

Protein Families

HGTP_anticodon2 Pkinase RWD tRNA-synt_His

Protein Analysis

1235

Amino Acids

139.08

Weight (kDa)

5.62

Isoelectric Point (pI)

41.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWD	PF05773	35 - 144	1.6e-18	RWD domain
Pkinase	PF00069	418 - 723	5.3e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	528 - 720	6.2e-15	Protein tyrosine and serine/threonine kinase
tRNA-synt_His	PF13393	869 - 1111	8.6e-11	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	1131 - 1225	7.9e-08	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	1132 - 1221	6.9e-07	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59410 AT3G59410 AT3G59410 AT3G59410 AT3G59410
fragaria_vesca	FvH4_4g10800 FvH4_4g10800
malus_domestica	MD04G1040400.v1.1
prunus_persica	Prupe.1G194100_v2.0.a1 Prupe.1G194100_v2.0.a1
pyrus_communis	pycom04g03520
rosa_chinensis	RchiOBHm_Chr4g0406781
rosa_laevigata	RLG00000008759
rosa_multiflora	Rmu_sc0006408.1_g000006
rosa_roxburghii	Rroxscaffold_5G00351540
rosa_rugosa	Rorug04G0072900
rosa_samantha	Rh4AG140400 Rh4BG137400 Rh4CG147300 Rh4DG134400
rosa_wichuraiana	Rw4G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2156
Acc36I	ACCTGC	1 cut(s)	2661
AccB1I	GGYRCC	2 cut(s)	1992, 2019
AccI	GTMKAC	1 cut(s)	2613
AccII	CGCG	1 cut(s)	30
AccIII	TCCGGA	2 cut(s)	164, 2083
AciI	CCGC	6 cut(s)	30, 1348, 1381, 2104, 2843, 2948
AclWI	GGATC	9 cut(s)	234, 954, 967, 987, 1829, 1836, 3122, 3214, 3491
AcoI	YGGCCR	3 cut(s)	1288, 1800, 1931
AcsI	RAATTY	7 cut(s)	367, 425, 631, 902, 2078, 2601, 3642
AcuI	CTGAAG	4 cut(s)	132, 556, 2066, 3635
AcyI	GRCGYC	1 cut(s)	2917
AfaI	GTAC	8 cut(s)	1219, 1434, 1537, 1592, 1607, 1879, 1894, 1961
AfiI	CCNNNNNNNGG	3 cut(s)	311, 1354, 2103
AflIII	ACRYGT	2 cut(s)	1426, 3115
AhlI	ACTAGT	1 cut(s)	3442
AjnI	CCWGG	8 cut(s)	265, 1448, 1490, 1626, 1698, 3174, 3202, 3298
Alw21I	GWGCWC	5 cut(s)	172, 499, 1194, 1477, 3226
Alw26I	GTCTC	6 cut(s)	241, 611, 1010, 1272, 3156, 3323
Alw44I	GTGCAC	1 cut(s)	3222
AlwI	GGATC	9 cut(s)	234, 954, 967, 987, 1829, 1836, 3122, 3214, 3491
AlwNI	CAGNNNCTG	2 cut(s)	872, 3569
Ama87I	CYCGRG	2 cut(s)	305, 378
Aor13HI	TCCGGA	2 cut(s)	164, 2083
AoxI	GGCC	8 cut(s)	197, 542, 743, 1288, 1446, 1696, 1800, 1931
ApaLI	GTGCAC	1 cut(s)	3222
ApeKI	GCWGC	5 cut(s)	416, 1508, 1822, 2539, 2996
ApoI	RAATTY	7 cut(s)	367, 425, 631, 902, 2078, 2601, 3642
ArsI	GACNNNNNNTTYG	2 cut(s)	3537, 3569
AspA2I	CCTAGG	2 cut(s)	650, 1613
AspLEI	GCGC	1 cut(s)	30
AspS9I	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3312, 3598
AsuC2I	CCSGG	1 cut(s)	85
AsuHPI	GGTGA	8 cut(s)	175, 857, 1493, 2063, 2575, 2672, 2728, 3199
AsuII	TTCGAA	2 cut(s)	14, 2570
AvaI	CYCGRG	2 cut(s)	305, 378
AvaII	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3312, 3598
AvrII	CCTAGG	2 cut(s)	650, 1613
AxyI	CCTNAGG	1 cut(s)	873
BaeGI	GKGCMC	1 cut(s)	3226
BaeI	ACNNNNGTAYC	2 cut(s)	254, 287
BalI	TGGCCA	3 cut(s)	1290, 1802, 1933
BamHI	GGATCC	1 cut(s)	959
BanI	GGYRCC	2 cut(s)	1992, 2019
BanII	GRGCYC	3 cut(s)	1194, 2867, 3031
BarI	GAAGNNNNNNTAC	6 cut(s)	2323, 2355, 2832, 2864, 3210, 3242
BauI	CACGAG	1 cut(s)	2376
BbsI	GAAGAC	2 cut(s)	2626, 2874
Bbv12I	GWGCWC	5 cut(s)	172, 499, 1194, 1477, 3226
BbvI	GCAGC	5 cut(s)	428, 1520, 1834, 2526, 2983
BccI	CCATC	9 cut(s)	194, 653, 1153, 1313, 1737, 1868, 2134, 2303, 2854
BcgI	CGANNNNNNTGC	4 cut(s)	1661, 1695, 2324, 2358
BciT130I	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
BciVI	GTATCC	1 cut(s)	282
BclI	TGATCA	2 cut(s)	358, 2709
BcnI	CCSGG	1 cut(s)	85
BcoDI	GTCTC	6 cut(s)	241, 611, 1010, 1272, 3156, 3323
BcuI	ACTAGT	1 cut(s)	3442
BfaI	CTAG	8 cut(s)	528, 651, 1316, 1373, 1614, 2195, 3015, 3443
BfmI	CTRYAG	8 cut(s)	1262, 1506, 1848, 2145, 2614, 2654, 2997, 3082
BfuAI	ACCTGC	1 cut(s)	2661
BfuI	GTATCC	1 cut(s)	282
BglII	AGATCT	1 cut(s)	3076
BisI	GCNGC	6 cut(s)	417, 1509, 1823, 2540, 2949, 2997
BlnI	CCTAGG	2 cut(s)	650, 1613
BlpI	GCTNAGC	1 cut(s)	2282
BlsI	GCNGC	6 cut(s)	418, 1510, 1824, 2541, 2950, 2998
BmcAI	AGTACT	1 cut(s)	1607
Bme1390I	CCNGG	9 cut(s)	85, 267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
Bme18I	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3312, 3598
BmeT110I	CYCGRG	2 cut(s)	305, 378
BmgT120I	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3312, 3598
BmiI	GGNNCC	9 cut(s)	465, 543, 664, 961, 1994, 2021, 2866, 3028, 3313
BmrFI	CCNGG	9 cut(s)	85, 267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
BmsI	GCATC	6 cut(s)	1024, 1753, 2102, 2268, 2693, 3655
BpiI	GAAGAC	2 cut(s)	2626, 2874
BplI	GAGNNNNNCTC	2 cut(s)	1854, 1886
BpmI	CTGGAG	2 cut(s)	1881, 2810
Bpu10I	CCTNAGC	2 cut(s)	194, 420
Bpu1102I	GCTNAGC	1 cut(s)	2282
Bpu14I	TTCGAA	2 cut(s)	14, 2570
BpuEI	CTTGAG	2 cut(s)	1337, 3112
BpuMI	CCSGG	1 cut(s)	85
BsaAI	YACGTR	1 cut(s)	1769
BsaBI	GATNNNNATC	2 cut(s)	1833, 3198
BsaHI	GRCGYC	1 cut(s)	2917
BsaI	GGTCTC	1 cut(s)	3323
BsaJI	CCNNGG	8 cut(s)	84, 266, 650, 942, 1491, 1613, 2506, 2776
BsaWI	WCCGGW	2 cut(s)	164, 2083
BsaXI	ACNNNNNCTCC	4 cut(s)	586, 616, 1250, 1280
Bsc4I	CCNNNNNNNGG	3 cut(s)	311, 1354, 2103
Bse1I	ACTGG	7 cut(s)	446, 467, 1112, 1864, 1885, 2793, 3574
Bse21I	CCTNAGG	1 cut(s)	873
Bse3DI	GCAATG	3 cut(s)	644, 2016, 3675
Bse8I	GATNNNNATC	2 cut(s)	1833, 3198
BseAI	TCCGGA	2 cut(s)	164, 2083
BseBI	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
BseDI	CCNNGG	8 cut(s)	84, 266, 650, 942, 1491, 1613, 2506, 2776
BseGI	GGATG	7 cut(s)	238, 664, 1166, 1725, 2093, 2467, 3670
BseJI	GATNNNNATC	2 cut(s)	1833, 3198
BseLI	CCNNNNNNNGG	3 cut(s)	311, 1354, 2103
BseMI	GCAATG	3 cut(s)	644, 2016, 3675
BseNI	ACTGG	7 cut(s)	446, 467, 1112, 1864, 1885, 2793, 3574
BseRI	GAGGAG	8 cut(s)	48, 51, 54, 57, 890, 1271, 2526, 3426
BseSI	GKGCMC	1 cut(s)	3226
BseXI	GCAGC	5 cut(s)	428, 1520, 1834, 2526, 2983
BsgI	GTGCAG	3 cut(s)	225, 997, 1893
Bsh1236I	CGCG	1 cut(s)	30
BshFI	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
BshNI	GGYRCC	2 cut(s)	1992, 2019
BsiHKAI	GWGCWC	5 cut(s)	172, 499, 1194, 1477, 3226
BsiHKCI	CYCGRG	2 cut(s)	305, 378
BsiSI	CCGG	3 cut(s)	84, 165, 2084
BslI	CCNNNNNNNGG	3 cut(s)	311, 1354, 2103
BsmAI	GTCTC	6 cut(s)	241, 611, 1010, 1272, 3156, 3323
BsmBI	CGTCTC	1 cut(s)	1010
BsmI	GAATGC	2 cut(s)	2263, 3343
BsnI	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
Bso31I	GGTCTC	1 cut(s)	3323
BsoBI	CYCGRG	2 cut(s)	305, 378
Bsp119I	TTCGAA	2 cut(s)	14, 2570
Bsp1286I	GDGCHC	7 cut(s)	172, 499, 1194, 1477, 2867, 3031, 3226
Bsp13I	TCCGGA	2 cut(s)	164, 2083
Bsp1407I	TGTACA	2 cut(s)	1590, 1959
Bsp1720I	GCTNAGC	1 cut(s)	2282
Bsp19I	CCATGG	1 cut(s)	2506
BspACI	CCGC	6 cut(s)	30, 1348, 1381, 2104, 2843, 2948
BspANI	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
BspEI	TCCGGA	2 cut(s)	164, 2083
BspFNI	CGCG	1 cut(s)	30
BspHI	TCATGA	2 cut(s)	949, 2305
BspLI	GGNNCC	9 cut(s)	465, 543, 664, 961, 1994, 2021, 2866, 3028, 3313
BspMAI	CTGCAG	4 cut(s)	1510, 1852, 2658, 3001
BspMI	ACCTGC	1 cut(s)	2661
BspPI	GGATC	9 cut(s)	234, 954, 967, 987, 1829, 1836, 3122, 3214, 3491
BspT104I	TTCGAA	2 cut(s)	14, 2570
BspT107I	GGYRCC	2 cut(s)	1992, 2019
BspTNI	GGTCTC	1 cut(s)	3323
BsrDI	GCAATG	3 cut(s)	644, 2016, 3675
BsrGI	TGTACA	2 cut(s)	1590, 1959
BsrI	ACTGG	7 cut(s)	446, 467, 1112, 1864, 1885, 2793, 3574
BssECI	CCNNGG	8 cut(s)	84, 266, 650, 942, 1491, 1613, 2506, 2776
BssNI	GRCGYC	1 cut(s)	2917
BssSI	CACGAG	1 cut(s)	2376
BssT1I	CCWWGG	4 cut(s)	650, 942, 1613, 2506
Bst2BI	CACGAG	1 cut(s)	2376
Bst2UI	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
Bst4CI	ACNGT	5 cut(s)	494, 640, 1266, 2491, 2974
Bst6I	CTCTTC	4 cut(s)	108, 575, 780, 2268
BstACI	GRCGYC	1 cut(s)	2917
BstAPI	GCANNNNNTGC	2 cut(s)	2539, 3230
BstAUI	TGTACA	2 cut(s)	1590, 1959
BstBAI	YACGTR	1 cut(s)	1769
BstBI	TTCGAA	2 cut(s)	14, 2570
BstC8I	GCNNGC	6 cut(s)	708, 1012, 1367, 1513, 1931, 2163
BstDSI	CCRYGG	2 cut(s)	2506, 2776
BstF5I	GGATG	7 cut(s)	238, 664, 1166, 1725, 2093, 2467, 3670
BstFNI	CGCG	1 cut(s)	30
BstHHI	GCGC	1 cut(s)	30
BstMAI	GTCTC	6 cut(s)	241, 611, 1010, 1272, 3156, 3323
BstMWI	GCNNNNNNNGC	8 cut(s)	131, 167, 1930, 2072, 2539, 2545, 3005, 3230
BstNI	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
BstNSI	RCATGY	3 cut(s)	2165, 2636, 3119
BstSCI	CCNGG	9 cut(s)	83, 265, 1448, 1490, 1626, 1698, 3174, 3202, 3298
BstSFI	CTRYAG	8 cut(s)	1262, 1506, 1848, 2145, 2614, 2654, 2997, 3082
BstSLI	GKGCMC	1 cut(s)	3226
BstUI	CGCG	1 cut(s)	30
BstV1I	GCAGC	5 cut(s)	428, 1520, 1834, 2526, 2983
BstV2I	GAAGAC	2 cut(s)	2626, 2874
BstX2I	RGATCY	7 cut(s)	226, 959, 979, 1834, 3076, 3127, 3496
BstXI	CCANNNNNNTGG	1 cut(s)	2905
BstYI	RGATCY	7 cut(s)	226, 959, 979, 1834, 3076, 3127, 3496
Bsu36I	CCTNAGG	1 cut(s)	873
BsuI	GTATCC	1 cut(s)	282
BsuRI	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
BtgI	CCRYGG	2 cut(s)	2506, 2776
BtsCI	GGATG	7 cut(s)	238, 664, 1166, 1725, 2093, 2467, 3670
BtsI	GCAGTG	4 cut(s)	55, 120, 516, 1905
BtsIMutI	CAGTG	9 cut(s)	55, 120, 453, 499, 516, 580, 1905, 2487, 3567
BveI	ACCTGC	1 cut(s)	2661
Cac8I	GCNNGC	6 cut(s)	708, 1012, 1367, 1513, 1931, 2163
CaiI	CAGNNNCTG	2 cut(s)	872, 3569
CciI	TCATGA	2 cut(s)	949, 2305
CfoI	GCGC	1 cut(s)	30
Cfr13I	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3312, 3598
CseI	GACGC	3 cut(s)	150, 2441, 2906
Csp6I	GTAC	8 cut(s)	1218, 1433, 1536, 1591, 1606, 1878, 1893, 1960
CviQI	GTAC	8 cut(s)	1218, 1433, 1536, 1591, 1606, 1878, 1893, 1960
DraI	TTTAAA	5 cut(s)	720, 1125, 1531, 3361, 3685
EaeI	YGGCCR	3 cut(s)	1288, 1800, 1931
Eam1104I	CTCTTC	4 cut(s)	108, 575, 780, 2268
EarI	CTCTTC	4 cut(s)	108, 575, 780, 2268
EciI	GGCGGA	1 cut(s)	1337
Ecl136II	GAGCTC	1 cut(s)	1192
Eco130I	CCWWGG	4 cut(s)	650, 942, 1613, 2506
Eco147I	AGGCCT	3 cut(s)	199, 1448, 1698
Eco24I	GRGCYC	3 cut(s)	1194, 2867, 3031
Eco31I	GGTCTC	1 cut(s)	3323
Eco32I	GATATC	2 cut(s)	1241, 3541
Eco47I	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3312, 3598
Eco53kI	GAGCTC	1 cut(s)	1192
Eco57I	CTGAAG	4 cut(s)	132, 556, 2066, 3635
Eco81I	CCTNAGG	1 cut(s)	873
Eco88I	CYCGRG	2 cut(s)	305, 378
EcoICRI	GAGCTC	1 cut(s)	1192
EcoO109I	RGGNCCY	1 cut(s)	542
EcoRI	GAATTC	1 cut(s)	902
EcoRII	CCWGG	8 cut(s)	265, 1448, 1490, 1626, 1698, 3174, 3202, 3298
EcoRV	GATATC	2 cut(s)	1241, 3541
EcoT14I	CCWWGG	4 cut(s)	650, 942, 1613, 2506
EcoT22I	ATGCAT	2 cut(s)	678, 2167
EcoT38I	GRGCYC	3 cut(s)	1194, 2867, 3031
ErhI	CCWWGG	4 cut(s)	650, 942, 1613, 2506
Esp3I	CGTCTC	1 cut(s)	1010
FalI	AAGNNNNNCTT	2 cut(s)	53, 85
FauNDI	CATATG	1 cut(s)	3267
FbaI	TGATCA	2 cut(s)	358, 2709
FblI	GTMKAC	1 cut(s)	2613
Fnu4HI	GCNGC	6 cut(s)	417, 1509, 1823, 2540, 2949, 2997
FokI	GGATG	7 cut(s)	245, 671, 1153, 1712, 2080, 2474, 3677
FriOI	GRGCYC	3 cut(s)	1194, 2867, 3031
Fsp4HI	GCNGC	6 cut(s)	417, 1509, 1823, 2540, 2949, 2997
FspBI	CTAG	8 cut(s)	528, 651, 1316, 1373, 1614, 2195, 3015, 3443
GlaI	GCGC	1 cut(s)	29
GluI	GCNGC	6 cut(s)	417, 1509, 1823, 2540, 2949, 2997
GsuI	CTGGAG	2 cut(s)	1881, 2810
HaeIII	GGCC	8 cut(s)	199, 544, 745, 1290, 1448, 1698, 1802, 1933
HapII	CCGG	3 cut(s)	84, 165, 2084
HgaI	GACGC	3 cut(s)	150, 2441, 2906
HhaI	GCGC	1 cut(s)	30
Hin1I	GRCGYC	1 cut(s)	2917
Hin6I	GCGC	1 cut(s)	28
HinP1I	GCGC	1 cut(s)	28
HincII	GTYRAC	1 cut(s)	613
HindII	GTYRAC	1 cut(s)	613
HindIII	AAGCTT	2 cut(s)	191, 2284
HpaII	CCGG	3 cut(s)	84, 165, 2084
HphI	GGTGA	8 cut(s)	175, 857, 1493, 2063, 2575, 2672, 2728, 3199
Hpy166II	GTNNAC	4 cut(s)	613, 2054, 2614, 3224
Hpy8I	GTNNAC	4 cut(s)	613, 2054, 2614, 3224
HpyCH4III	ACNGT	5 cut(s)	494, 640, 1266, 2491, 2974
HpyCH4IV	ACGT	3 cut(s)	1428, 1768, 2872
HpyF10VI	GCNNNNNNNGC	8 cut(s)	131, 167, 1930, 2072, 2539, 2545, 3005, 3230
HpySE526I	ACGT	3 cut(s)	1428, 1768, 2872
Hsp92I	GRCGYC	1 cut(s)	2917
HspAI	GCGC	1 cut(s)	28
Kpn2I	TCCGGA	2 cut(s)	164, 2083
Ksp22I	TGATCA	2 cut(s)	358, 2709
Lsp1109I	GCAGC	5 cut(s)	428, 1520, 1834, 2526, 2983
LweI	GCATC	6 cut(s)	1024, 1753, 2102, 2268, 2693, 3655
MaeI	CTAG	8 cut(s)	528, 651, 1316, 1373, 1614, 2195, 3015, 3443
MaeII	ACGT	3 cut(s)	1428, 1768, 2872
MaeIII	GTNAC	4 cut(s)	326, 530, 2716, 2731
MfeI	CAATTG	3 cut(s)	2625, 2746, 3234
MflI	RGATCY	7 cut(s)	226, 959, 979, 1834, 3076, 3127, 3496
MhlI	GDGCHC	7 cut(s)	172, 499, 1194, 1477, 2867, 3031, 3226
MlsI	TGGCCA	3 cut(s)	1290, 1802, 1933
MluNI	TGGCCA	3 cut(s)	1290, 1802, 1933
MlyI	GAGTC	4 cut(s)	323, 917, 1646, 2823
MmeI	TCCRAC	6 cut(s)	578, 801, 900, 1797, 2058, 2439
Mox20I	TGGCCA	3 cut(s)	1290, 1802, 1933
Mph1103I	ATGCAT	2 cut(s)	678, 2167
MroI	TCCGGA	2 cut(s)	164, 2083
MscI	TGGCCA	3 cut(s)	1290, 1802, 1933
MslI	CAYNNNNRTG	3 cut(s)	1598, 1710, 2307
Msp20I	TGGCCA	3 cut(s)	1290, 1802, 1933
MspA1I	CMGCKG	1 cut(s)	1825
MspI	CCGG	3 cut(s)	84, 165, 2084
MspR9I	CCNGG	9 cut(s)	85, 267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
MunI	CAATTG	3 cut(s)	2625, 2746, 3234
Mva1269I	GAATGC	2 cut(s)	2263, 3343
MvaI	CCWGG	8 cut(s)	267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
MvnI	CGCG	1 cut(s)	30
MwoI	GCNNNNNNNGC	8 cut(s)	131, 167, 1930, 2072, 2539, 2545, 3005, 3230
NciI	CCSGG	1 cut(s)	85
NcoI	CCATGG	1 cut(s)	2506
NdeI	CATATG	1 cut(s)	3267
NlaIV	GGNNCC	9 cut(s)	465, 543, 664, 961, 1994, 2021, 2866, 3028, 3313
NmuCI	GTSAC	2 cut(s)	326, 2716
NsiI	ATGCAT	2 cut(s)	678, 2167
NspI	RCATGY	3 cut(s)	2165, 2636, 3119
NspV	TTCGAA	2 cut(s)	14, 2570
PaeI	GCATGC	1 cut(s)	2165
PagI	TCATGA	2 cut(s)	949, 2305
PceI	AGGCCT	3 cut(s)	199, 1448, 1698
PciI	ACATGT	1 cut(s)	3115
PctI	GAATGC	2 cut(s)	2263, 3343
PfeI	GAWTC	9 cut(s)	682, 803, 881, 1577, 1722, 2183, 2347, 2366, 2572
PfoI	TCCNGGA	1 cut(s)	3202
PkrI	GCNGC	6 cut(s)	418, 1510, 1824, 2541, 2950, 2998
PleI	GAGTC	4 cut(s)	323, 917, 1645, 2822
PpsI	GAGTC	4 cut(s)	323, 917, 1645, 2822
Ppu21I	YACGTR	1 cut(s)	1769
PscI	ACATGT	1 cut(s)	3115
PsiI	TTATAA	1 cut(s)	2156
Psp124BI	GAGCTC	1 cut(s)	1194
Psp6I	CCWGG	8 cut(s)	265, 1448, 1490, 1626, 1698, 3174, 3202, 3298
PspGI	CCWGG	8 cut(s)	265, 1448, 1490, 1626, 1698, 3174, 3202, 3298
PspN4I	GGNNCC	9 cut(s)	465, 543, 664, 961, 1994, 2021, 2866, 3028, 3313
PspPI	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3312, 3598
PsrI	GAACNNNNNNTAC	2 cut(s)	2925, 2957
PstI	CTGCAG	4 cut(s)	1510, 1852, 2658, 3001
PstNI	CAGNNNCTG	2 cut(s)	872, 3569
PsuI	RGATCY	7 cut(s)	226, 959, 979, 1834, 3076, 3127, 3496
PvuII	CAGCTG	1 cut(s)	1825
RsaI	GTAC	8 cut(s)	1219, 1434, 1537, 1592, 1607, 1879, 1894, 1961
RsaNI	GTAC	8 cut(s)	1218, 1433, 1536, 1591, 1606, 1878, 1893, 1960
RseI	CAYNNNNRTG	3 cut(s)	1598, 1710, 2307
SacI	GAGCTC	1 cut(s)	1194
SatI	GCNGC	6 cut(s)	417, 1509, 1823, 2540, 2949, 2997
Sau96I	GGNCC	8 cut(s)	464, 542, 663, 743, 1022, 1211, 3312, 3598
SbfI	CCTGCAGG	1 cut(s)	2658
ScaI	AGTACT	1 cut(s)	1607
SchI	GAGTC	4 cut(s)	323, 917, 1646, 2823
ScrFI	CCNGG	9 cut(s)	85, 267, 1450, 1492, 1628, 1700, 3176, 3204, 3300
SdaI	CCTGCAGG	1 cut(s)	2658
SduI	GDGCHC	7 cut(s)	172, 499, 1194, 1477, 2867, 3031, 3226
SfaNI	GCATC	6 cut(s)	1024, 1753, 2102, 2268, 2693, 3655
SfcI	CTRYAG	8 cut(s)	1262, 1506, 1848, 2145, 2614, 2654, 2997, 3082
SfuI	TTCGAA	2 cut(s)	14, 2570
SinI	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3312, 3598
SmiMI	CAYNNNNRTG	3 cut(s)	1598, 1710, 2307
SmlI	CTYRAG	2 cut(s)	1352, 3091
SmoI	CTYRAG	2 cut(s)	1352, 3091
SpeI	ACTAGT	1 cut(s)	3442
SphI	GCATGC	1 cut(s)	2165
Sse8387I	CCTGCAGG	1 cut(s)	2658
SseBI	AGGCCT	3 cut(s)	199, 1448, 1698
SsiI	CCGC	6 cut(s)	30, 1348, 1381, 2104, 2843, 2948
SspI	AATATT	2 cut(s)	138, 2203
SspMI	CTAG	8 cut(s)	528, 651, 1316, 1373, 1614, 2195, 3015, 3443
SstI	GAGCTC	1 cut(s)	1194
StuI	AGGCCT	3 cut(s)	199, 1448, 1698
StyD4I	CCNGG	9 cut(s)	83, 265, 1448, 1490, 1626, 1698, 3174, 3202, 3298
StyI	CCWWGG	4 cut(s)	650, 942, 1613, 2506
TaaI	ACNGT	5 cut(s)	494, 640, 1266, 2491, 2974
TaiI	ACGT	3 cut(s)	1431, 1771, 2875
TaqI	TCGA	8 cut(s)	14, 411, 1129, 1237, 1872, 1983, 2570, 2667
TatI	WGTACW	4 cut(s)	1217, 1590, 1605, 1959
TauI	GCSGC	1 cut(s)	2951
TfiI	GAWTC	9 cut(s)	682, 803, 881, 1577, 1722, 2183, 2347, 2366, 2572
TscAI	CASTG	9 cut(s)	55, 127, 453, 499, 523, 580, 1912, 2494, 3574
TseFI	GTSAC	2 cut(s)	326, 2716
TseI	GCWGC	5 cut(s)	416, 1508, 1822, 2539, 2996
Tsp45I	GTSAC	2 cut(s)	326, 2716
TspGWI	ACGGA	5 cut(s)	992, 2905, 3067, 3524, 3590
TspRI	CASTG	9 cut(s)	55, 127, 453, 499, 523, 580, 1912, 2494, 3574
VneI	GTGCAC	1 cut(s)	3222
VpaK11BI	GGWCC	6 cut(s)	464, 663, 1022, 1211, 3312, 3598
XapI	RAATTY	7 cut(s)	367, 425, 631, 902, 2078, 2601, 3642
XceI	RCATGY	3 cut(s)	2165, 2636, 3119
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2455
XmaJI	CCTAGG	2 cut(s)	650, 1613
XmiI	GTMKAC	1 cut(s)	2613
XspI	CTAG	8 cut(s)	528, 651, 1316, 1373, 1614, 2195, 3015, 3443
ZrmI	AGTACT	1 cut(s)	1607
Zsp2I	ATGCAT	2 cut(s)	678, 2167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.