Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0010438

Physical Location & Seq

Genomic Coordinates Forward (+)

36474418 .. 36493679

Gene Structure 19262 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000008759

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3711 bp

ATGGGACATAGTTCGAAGAAGAAGAAGCGCGGAGGAGGAGGAGGCAGTGGGAAGAAGAAGAAGCCCTTGAAAGATCATGCCGAGGCTTACGATAATGACCTTCTTTCTGAAGAGATCACTGCTTTGTGTGCAATATTTCAAGATGACTGCAAAATTGCGTCCGGATCACAACCTCAAATCACCATCAAGCTTAGGCCTCACTCAAAGGATATGGGTTATGAGGATCTGGATGTCTCTGCACTTCTTTTAGTTAGGTGCTTACCAGGGTATCCTTACAAGTGTCCCAAGTTGCAGATAACTCCAGAGAAAGGATTATCGAAAAGTGACTCTGATAAGCTATTATCTCTTATCAATGATCAGGCAAATTCCAATGCCCGAGAAGGACGGGTAATGATCTTCAATTTGGTCGAGGCAGCTCAGGAATTTCTATCTGAAATTGTGCCAGTGGGTCAATCACAGGGACCAGTAATATGTTCAACTACGGATAACAGTGCTCAGTTGTTTCAAAATGACACTGCAATTTCTAGTAACAAGAAGGGGCCTTTTGTTTATGGTTTCATAGACCTCTTCAGTGGCTCTGGAAAGTCTTGGAGTTGGAGTTTTGGAGTTGATGAGACTAAAGGGATAAATTCTACTGTCCCATTGCCTAGGGTGGATGGGTCCAAACTCATGCATGAGATTCAGGAAAAGAAACATGATAAGCAAACAGAACCTTTAAAATCACAAGATATAAAGCAAGGGCCAGTACTTTCCCCTAATGTCAAGTTAGAAACGCTTGAAGAAGAGAGTGAGGGCAGGGATTCAAGTAGATTTTTGTTGGAAGAAATGGACAGCAATGGTGGTGAACCTGAGAAAGAGAGTTCAGTTCTTGAGGAGGATTCAACAGAAGATGATTGGGAATTTGGAAGTCAGCAGTCGGACTCACTCTCTTTTACTTCCTTGGCTGATGAGCAAGGATCCCAAAATGTTAAAAGGGATCTAATAATGGTTCATCTGCTCCGTCTCGCCTGCACTAAAGGACCATTGGCTGATGCTTTGCCGCAGATAACCACAGAACTGCAAAACATAGGGGTCTTATCAGAATGGGCAAGGGATTTAGCTTCAAAACCAATTTCACTTTTAAATCGAACATTTAATCATGCCTTTGGACAACATATGGTTTCATCCCGTATCTCTCAATTCTGGGAGCTCACTTCTGATTTAGAAGGGCCAAGTACATCTCTCCCAAGTTCTCGATATCTCAACGACTTTGAGGAGCTACAGTCTCTTGGTCATGGTGGCTTTGGCCATGTTGTGTTGTGCAAAAATAAGCTAGATGGAAGGCAGTATGCAGTAAAGAAAATCCGCCTTAAGGATAAAAGCCTGCCACTTGATGACCGCATATTGAGGGAAGTCGCTACACTTTCTCGTTTGCAACATCAACACGTTGTACGATACTATCAGGCCTGGTTTGAAACAGGAGGCGTTGGTGCTCATGGTGATACTACTTGGGGTTCAAGGACTGCAGCCAGCTCCACTTTCAGCTTTAAAGGTACAAGCTCAGCAGATGATCTTGGGAATGAGAATAAACTTGAATCAACATATTTGTACATTCAAATGGAGTACTGCCCTAGGACATTACGCCAGGTTTTTGAGTCGTATAGTCAGTTTGACAAAGAATTAGCATGGCATTTGTTTCGTCAAATTGTTGAAGGCCTGGCACACATACATGGACAAGGAATCATCCATCGGGACTTAACGCCAAATAACATATTCTTTGATGCACGTAATGATATTAAAATTGGAGATTTTGGTTTGGCCAAGTTTTTGAAGTTGGAGCAGCTGGATCAAGATCCAAGTTTTCCTGCAGATACAACTGGAGTTTCTCTCGATGGTACTGGTCAAGTTGGTACATATTTTTACACCGCACCTGAAATTGAGCAAGGCTGGCCAAAGATTGATGAAAAGGCTGATATGTACAGCTTGGGAATTGTATTTTTCGAGCTATGGCACCCATTTGGGACTGCAATGGAACGGCACTTTGTCCTATCCGACTTGAAACAGAAGGGTGAACTGCCTTCTGCTTGGGTTGCTGAATTTCCGGAACAAGCATCGTTGTTGCGGAGCTTAATGTCCCCAAGTCCGTCAGATCGCCCTTCTGCTACAGAACTTATAAAGCATGCATTTCCACCACGAATGGAATCTGAGTTGCTAGACAATATTCTACGAACAATGCAAACCTCAGAGGACAGAAGTGTGTATGATAAAGTTCTGAATGCTATATTTGATGAAGAGATGCTAAGCTTGAAAGACCAGCAACATCATGATGGTAGGTTGAGGTTGGCTGGTAGCGATACTTCTGCGATTCAGTATGCAGATTTAGATTCAGAGGCTCGTGATTATGTTGTTGAGATCACAAGGGAGGTGTTTAGACAACATTGTGCAAAGCACCTTGAAGTAATACCCATGCGTCTGTTGGATGATTGCCAACATTTTATGAGAAGGAACACTGTCAAACTTTTGACCCATGGAGGAGATGTGCTTGAACTTTTGCATGAGCTGCGTTTGCCTTTTGTTGGTTGGGTGATTTCGAATCAGAAATCTTCATTCAAAAGATATGAAATTTCATGTGTCTACAGAAGACCAATTGGACATGCACCCCCAAGTCGCTACCTGCAGGGTGATTTCGACATTATTGGAGGTGCATCAGCATTGACAGAGGCAGAAGTGATCAAGGTGACAAGGGACATTGTAACCCGTTTTTTCAATTGGGAGTTCTGCGACATTCATCTAAACCACGGGGACTTACTGGAGGCAATTTGGTCTTGGGTAGGAGTCAAGGCAGACCATAGACAAAAAGTAGCGGAGCTTCTTTCTATTATGGGCTCCTTACGTCCTCAGTCTTCTGAACGGAAATCCAAATGGGTGGTAATAAGGCGTCAGCTTTTGCAGGAACTTAATTTACAAGAGGCTGTTGTGAATAGGTTGCAGACTGTTGGTTTAAGGTTCTGTGGAGCTGCAGATCAGGCACTTCCTAGACTTAGGGGAGCCCTGCCAAACGATAAACCAACACGGAAGGCACTTGATGAGTTGTCAGATCTCTGTAGCTACTTGAGAGCTTGGAGGATTGAAGAACATGTTTATATAGATCCACTTATCCCCCCAACAGAGAGTTATCATAGAGACTTGTTTTTCCAGGTATATTTGGTAAAGGATAGTAATCCTGGATCACTTACTGAAGGTGCACTACTTGCAATTGGGGGTCGGTATGACTATTTGCTTCATCATATGTGGGGCCTTGAACATAAATCTAATCCTCCTGGTTCTGTTGGGACCAGCCTTGCTTTAGAGACCATAATTCAGCATTCTCCTGTGGATTTTAAACCAATTAGCAGAAATGAAGTTAGCAATAGTGTTCTTGTTTGTTCAAAAGGCGGAGGGGGTCTATTGGCAGAACGCATGGAGCTAGTCAATGAGCTTTGGGAAGAAAACATTAAGGCTGAGTTTCTCCCAACACCAGATCCAAGCCTTACGGAGCAGTATGAATATGCCAACGAACATGATATTAAATGTCTTGTCATCATAACAGACACTGGTGTTTCTCAGAAAGGTTCTGTTAAGGTCCGTCATCTTGAACTGAAGAAGGAAAAGGAGGTTGAAAGGGAAATTCTCGTCAGGTTTCTGCTGGATGCAATGGCAATACAATTTAAAAATCCTTCCATTTGGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0014070 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034198 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0046677 GO:0050896 GO:0051716 GO:0060992 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928

KEGG Pathways

Metabolic & Signaling

ko04138 ko04140 ko04141 ko05160 ko05162 ko05164 ko05168 ko05169 map04138 map04140 map04141 map05160 map05162 map05164 map05168 map05169

Pfam Domains

Protein Families

HGTP_anticodon2 Pkinase RWD tRNA-synt_His

Protein Analysis

1237

Amino Acids

139.28

Weight (kDa)

5.6

Isoelectric Point (pI)

43.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWD	PF05773	34 - 143	6.9e-19	RWD domain
Pkinase	PF00069	417 - 722	1.4e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	527 - 719	5.5e-16	Protein tyrosine and serine/threonine kinase
tRNA-synt_His	PF13393	869 - 1111	6.5e-11	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	1132 - 1226	3.2e-09	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	1133 - 1222	2.3e-07	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59410 AT3G59410 AT3G59410 AT3G59410 AT3G59410
fragaria_vesca	FvH4_4g10800 FvH4_4g10800
malus_domestica	MD04G1040400.v1.1
prunus_persica	Prupe.1G194100_v2.0.a1 Prupe.1G194100_v2.0.a1
pyrus_communis	pycom04g03520
rosa_chinensis	RchiOBHm_Chr4g0406781
rosa_laevigata	RLG00000008759
rosa_multiflora	Rmu_sc0006408.1_g000006
rosa_roxburghii	Rroxscaffold_5G00351540
rosa_rugosa	Rorug04G0072900
rosa_samantha	Rh4AG140400 Rh4BG137400 Rh4CG147300 Rh4DG134400
rosa_wichuraiana	Rw4G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2153
Acc36I	ACCTGC	1 cut(s)	2661
AccB1I	GGYRCC	1 cut(s)	1989
AccI	GTMKAC	1 cut(s)	2613
AccII	CGCG	1 cut(s)	30
AccIII	TCCGGA	2 cut(s)	161, 2080
AciI	CCGC	8 cut(s)	30, 1040, 1345, 1378, 1905, 2101, 2843, 3414
AcoI	YGGCCR	3 cut(s)	1285, 1797, 1928
AcsI	RAATTY	7 cut(s)	364, 422, 628, 899, 2075, 2601, 3645
AcuI	CTGAAG	4 cut(s)	129, 553, 3237, 3638
AcyI	GRCGYC	1 cut(s)	2917
AfaI	GTAC	9 cut(s)	747, 1216, 1431, 1534, 1589, 1604, 1876, 1891, 1958
AfiI	CCNNNNNNNGG	3 cut(s)	308, 1351, 2555
AflII	CTTAAG	1 cut(s)	1349
AflIII	ACRYGT	2 cut(s)	1423, 3115
AjnI	CCWGG	7 cut(s)	262, 1445, 1623, 1695, 3174, 3202, 3298
Alw21I	GWGCWC	4 cut(s)	496, 1191, 1474, 3226
Alw26I	GTCTC	6 cut(s)	238, 608, 1007, 1269, 3156, 3323
Alw44I	GTGCAC	1 cut(s)	3222
AlwNI	CAGNNNCTG	1 cut(s)	3572
Ama87I	CYCGRG	1 cut(s)	375
Aor13HI	TCCGGA	2 cut(s)	161, 2080
ApaLI	GTGCAC	1 cut(s)	3222
ApeKI	GCWGC	5 cut(s)	413, 1505, 1819, 2539, 2996
ApoI	RAATTY	7 cut(s)	364, 422, 628, 899, 2075, 2601, 3645
AspA2I	CCTAGG	2 cut(s)	647, 1610
AspLEI	GCGC	1 cut(s)	30
AspS9I	GGNCC	9 cut(s)	461, 539, 660, 740, 1019, 1208, 3274, 3312, 3601
AsuHPI	GGTGA	7 cut(s)	172, 854, 1490, 2060, 2575, 2672, 2728
AsuII	TTCGAA	2 cut(s)	14, 2570
AvaI	CYCGRG	1 cut(s)	375
AvaII	GGWCC	5 cut(s)	461, 660, 1019, 3312, 3601
AvrII	CCTAGG	2 cut(s)	647, 1610
BaeGI	GKGCMC	1 cut(s)	3226
BaeI	ACNNNNGTAYC	2 cut(s)	251, 284
BalI	TGGCCA	3 cut(s)	1287, 1799, 1930
BamHI	GGATCC	1 cut(s)	956
BanI	GGYRCC	1 cut(s)	1989
BanII	GRGCYC	3 cut(s)	1191, 2867, 3031
BarI	GAAGNNNNNNTAC	6 cut(s)	2320, 2352, 2832, 2864, 3210, 3242
BauI	CACGAG	1 cut(s)	2373
BbsI	GAAGAC	2 cut(s)	2626, 2874
Bbv12I	GWGCWC	4 cut(s)	496, 1191, 1474, 3226
BbvI	GCAGC	5 cut(s)	425, 1517, 1831, 2526, 2983
BccI	CCATC	6 cut(s)	191, 650, 1310, 1734, 1865, 2300
BceAI	ACGGC	1 cut(s)	2030
BcgI	CGANNNNNNTGC	4 cut(s)	1658, 1692, 2321, 2355
BciT130I	CCWGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
BciVI	GTATCC	1 cut(s)	279
BclI	TGATCA	2 cut(s)	355, 2709
BcoDI	GTCTC	6 cut(s)	238, 608, 1007, 1269, 3156, 3323
BfaI	CTAG	7 cut(s)	525, 648, 1313, 1611, 2192, 3015, 3446
BfmI	CTRYAG	8 cut(s)	1259, 1503, 1845, 2142, 2614, 2654, 2997, 3082
BfrI	CTTAAG	1 cut(s)	1349
BfuAI	ACCTGC	1 cut(s)	2661
BfuI	GTATCC	1 cut(s)	279
BglII	AGATCT	1 cut(s)	3076
BisI	GCNGC	6 cut(s)	414, 1040, 1506, 1820, 2540, 2997
BlnI	CCTAGG	2 cut(s)	647, 1610
BlpI	GCTNAGC	2 cut(s)	1540, 2279
BlsI	GCNGC	6 cut(s)	415, 1041, 1507, 1821, 2541, 2998
BmcAI	AGTACT	2 cut(s)	747, 1604
Bme1390I	CCNGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
Bme18I	GGWCC	5 cut(s)	461, 660, 1019, 3312, 3601
BmeT110I	CYCGRG	1 cut(s)	375
BmgT120I	GGNCC	9 cut(s)	461, 539, 660, 740, 1019, 1208, 3274, 3312, 3601
BmiI	GGNNCC	9 cut(s)	462, 540, 661, 958, 1991, 2866, 3028, 3275, 3313
BmrFI	CCNGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
BmsI	GCATC	6 cut(s)	1021, 1750, 2099, 2265, 2693, 3658
BpiI	GAAGAC	2 cut(s)	2626, 2874
BplI	GAGNNNNNCTC	2 cut(s)	1851, 1883
BpmI	CTGGAG	3 cut(s)	285, 1878, 2810
Bpu10I	CCTNAGC	2 cut(s)	191, 417
Bpu1102I	GCTNAGC	2 cut(s)	1540, 2279
Bpu14I	TTCGAA	2 cut(s)	14, 2570
BpuEI	CTTGAG	2 cut(s)	890, 3112
BsaAI	YACGTR	1 cut(s)	1766
BsaBI	GATNNNNATC	2 cut(s)	1830, 3198
BsaHI	GRCGYC	1 cut(s)	2917
BsaI	GGTCTC	1 cut(s)	3323
BsaJI	CCNNGG	7 cut(s)	81, 263, 647, 939, 1610, 2506, 2776
BsaWI	WCCGGW	2 cut(s)	161, 2080
BsaXI	ACNNNNNCTCC	6 cut(s)	583, 613, 1247, 1277, 2502, 2532
Bsc4I	CCNNNNNNNGG	3 cut(s)	308, 1351, 2555
Bse1I	ACTGG	7 cut(s)	443, 464, 743, 1861, 1882, 2793, 3577
Bse3DI	GCAATG	4 cut(s)	641, 841, 2013, 3678
Bse8I	GATNNNNATC	2 cut(s)	1830, 3198
BseAI	TCCGGA	2 cut(s)	161, 2080
BseBI	CCWGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
BseDI	CCNNGG	7 cut(s)	81, 263, 647, 939, 1610, 2506, 2776
BseGI	GGATG	6 cut(s)	235, 661, 1163, 1722, 2464, 3673
BseJI	GATNNNNATC	2 cut(s)	1830, 3198
BseLI	CCNNNNNNNGG	3 cut(s)	308, 1351, 2555
BseMI	GCAATG	4 cut(s)	641, 841, 2013, 3678
BseMII	CTCAG	9 cut(s)	431, 509, 840, 1554, 2175, 2235, 2891, 3471, 3596
BseNI	ACTGG	7 cut(s)	443, 464, 743, 1861, 1882, 2793, 3577
BseRI	GAGGAG	6 cut(s)	48, 51, 54, 887, 1268, 2526
BseSI	GKGCMC	1 cut(s)	3226
BseXI	GCAGC	5 cut(s)	425, 1517, 1831, 2526, 2983
BsgI	GTGCAG	2 cut(s)	222, 994
Bsh1236I	CGCG	1 cut(s)	30
BshNI	GGYRCC	1 cut(s)	1989
BsiHKAI	GWGCWC	4 cut(s)	496, 1191, 1474, 3226
BsiHKCI	CYCGRG	1 cut(s)	375
BsiSI	CCGG	2 cut(s)	162, 2081
BslI	CCNNNNNNNGG	3 cut(s)	308, 1351, 2555
BsmAI	GTCTC	6 cut(s)	238, 608, 1007, 1269, 3156, 3323
BsmBI	CGTCTC	1 cut(s)	1007
BsmI	GAATGC	2 cut(s)	2260, 3343
Bso31I	GGTCTC	1 cut(s)	3323
BsoBI	CYCGRG	1 cut(s)	375
Bsp119I	TTCGAA	2 cut(s)	14, 2570
Bsp1286I	GDGCHC	6 cut(s)	496, 1191, 1474, 2867, 3031, 3226
Bsp13I	TCCGGA	2 cut(s)	161, 2080
Bsp1407I	TGTACA	2 cut(s)	1587, 1956
Bsp1720I	GCTNAGC	2 cut(s)	1540, 2279
Bsp19I	CCATGG	1 cut(s)	2506
BspACI	CCGC	8 cut(s)	30, 1040, 1345, 1378, 1905, 2101, 2843, 3414
BspCNI	CTCAG	9 cut(s)	430, 508, 841, 1553, 2176, 2234, 2890, 3472, 3595
BspEI	TCCGGA	2 cut(s)	161, 2080
BspFNI	CGCG	1 cut(s)	30
BspHI	TCATGA	1 cut(s)	2302
BspLI	GGNNCC	9 cut(s)	462, 540, 661, 958, 1991, 2866, 3028, 3275, 3313
BspMAI	CTGCAG	4 cut(s)	1507, 1849, 2658, 3001
BspMI	ACCTGC	1 cut(s)	2661
BspT104I	TTCGAA	2 cut(s)	14, 2570
BspT107I	GGYRCC	1 cut(s)	1989
BspTI	CTTAAG	1 cut(s)	1349
BspTNI	GGTCTC	1 cut(s)	3323
BsrDI	GCAATG	4 cut(s)	641, 841, 2013, 3678
BsrGI	TGTACA	2 cut(s)	1587, 1956
BsrI	ACTGG	7 cut(s)	443, 464, 743, 1861, 1882, 2793, 3577
BssECI	CCNNGG	7 cut(s)	81, 263, 647, 939, 1610, 2506, 2776
BssNI	GRCGYC	1 cut(s)	2917
BssSI	CACGAG	1 cut(s)	2373
BssT1I	CCWWGG	4 cut(s)	647, 939, 1610, 2506
Bst2BI	CACGAG	1 cut(s)	2373
Bst2UI	CCWGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
Bst4CI	ACNGT	5 cut(s)	491, 637, 1263, 2491, 2974
Bst6I	CTCTTC	4 cut(s)	105, 572, 777, 2265
BstACI	GRCGYC	1 cut(s)	2917
BstAFI	CTTAAG	1 cut(s)	1349
BstAPI	GCANNNNNTGC	2 cut(s)	2539, 3230
BstAUI	TGTACA	2 cut(s)	1587, 1956
BstBAI	YACGTR	1 cut(s)	1766
BstBI	TTCGAA	2 cut(s)	14, 2570
BstC8I	GCNNGC	5 cut(s)	1009, 1364, 1510, 1928, 2160
BstDSI	CCRYGG	2 cut(s)	2506, 2776
BstF5I	GGATG	6 cut(s)	235, 661, 1163, 1722, 2464, 3673
BstFNI	CGCG	1 cut(s)	30
BstHHI	GCGC	1 cut(s)	30
BstMAI	GTCTC	6 cut(s)	238, 608, 1007, 1269, 3156, 3323
BstMWI	GCNNNNNNNGC	7 cut(s)	128, 1927, 2069, 2539, 2545, 3005, 3230
BstNI	CCWGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
BstNSI	RCATGY	3 cut(s)	2162, 2636, 3119
BstSCI	CCNGG	7 cut(s)	262, 1445, 1623, 1695, 3174, 3202, 3298
BstSFI	CTRYAG	8 cut(s)	1259, 1503, 1845, 2142, 2614, 2654, 2997, 3082
BstSLI	GKGCMC	1 cut(s)	3226
BstUI	CGCG	1 cut(s)	30
BstV1I	GCAGC	5 cut(s)	425, 1517, 1831, 2526, 2983
BstV2I	GAAGAC	2 cut(s)	2626, 2874
BstX2I	RGATCY	7 cut(s)	223, 956, 976, 1831, 3076, 3127, 3499
BstXI	CCANNNNNNTGG	1 cut(s)	2905
BstYI	RGATCY	7 cut(s)	223, 956, 976, 1831, 3076, 3127, 3499
BsuI	GTATCC	1 cut(s)	279
BtgI	CCRYGG	2 cut(s)	2506, 2776
BtsCI	GGATG	6 cut(s)	235, 661, 1163, 1722, 2464, 3673
BtsI	GCAGTG	3 cut(s)	52, 117, 513
BtsIMutI	CAGTG	8 cut(s)	52, 117, 450, 496, 513, 577, 2487, 3570
BveI	ACCTGC	1 cut(s)	2661
Cac8I	GCNNGC	5 cut(s)	1009, 1364, 1510, 1928, 2160
CaiI	CAGNNNCTG	1 cut(s)	3572
CciI	TCATGA	1 cut(s)	2302
CfoI	GCGC	1 cut(s)	30
Cfr13I	GGNCC	9 cut(s)	461, 539, 660, 740, 1019, 1208, 3274, 3312, 3601
CseI	GACGC	3 cut(s)	147, 2438, 2906
Csp6I	GTAC	9 cut(s)	746, 1215, 1430, 1533, 1588, 1603, 1875, 1890, 1957
CviQI	GTAC	9 cut(s)	746, 1215, 1430, 1533, 1588, 1603, 1875, 1890, 1957
DraI	TTTAAA	5 cut(s)	717, 1122, 1528, 3361, 3688
EaeI	YGGCCR	3 cut(s)	1285, 1797, 1928
Eam1104I	CTCTTC	4 cut(s)	105, 572, 777, 2265
EarI	CTCTTC	4 cut(s)	105, 572, 777, 2265
EciI	GGCGGA	2 cut(s)	1334, 3429
Ecl136II	GAGCTC	1 cut(s)	1189
Eco130I	CCWWGG	4 cut(s)	647, 939, 1610, 2506
Eco147I	AGGCCT	3 cut(s)	196, 1445, 1695
Eco24I	GRGCYC	3 cut(s)	1191, 2867, 3031
Eco31I	GGTCTC	1 cut(s)	3323
Eco32I	GATATC	1 cut(s)	1238
Eco47I	GGWCC	5 cut(s)	461, 660, 1019, 3312, 3601
Eco53kI	GAGCTC	1 cut(s)	1189
Eco57I	CTGAAG	4 cut(s)	129, 553, 3237, 3638
Eco88I	CYCGRG	1 cut(s)	375
EcoICRI	GAGCTC	1 cut(s)	1189
EcoO109I	RGGNCCY	2 cut(s)	539, 3274
EcoRII	CCWGG	7 cut(s)	262, 1445, 1623, 1695, 3174, 3202, 3298
EcoRV	GATATC	1 cut(s)	1238
EcoT14I	CCWWGG	4 cut(s)	647, 939, 1610, 2506
EcoT22I	ATGCAT	2 cut(s)	675, 2164
EcoT38I	GRGCYC	3 cut(s)	1191, 2867, 3031
ErhI	CCWWGG	4 cut(s)	647, 939, 1610, 2506
Esp3I	CGTCTC	1 cut(s)	1007
FalI	AAGNNNNNCTT	2 cut(s)	50, 82
FauNDI	CATATG	2 cut(s)	1155, 3267
FbaI	TGATCA	2 cut(s)	355, 2709
FblI	GTMKAC	1 cut(s)	2613
Fnu4HI	GCNGC	6 cut(s)	414, 1040, 1506, 1820, 2540, 2997
FokI	GGATG	6 cut(s)	242, 668, 1150, 1709, 2471, 3680
FriOI	GRGCYC	3 cut(s)	1191, 2867, 3031
Fsp4HI	GCNGC	6 cut(s)	414, 1040, 1506, 1820, 2540, 2997
FspBI	CTAG	7 cut(s)	525, 648, 1313, 1611, 2192, 3015, 3446
GlaI	GCGC	1 cut(s)	29
GluI	GCNGC	6 cut(s)	414, 1040, 1506, 1820, 2540, 2997
GsuI	CTGGAG	3 cut(s)	285, 1878, 2810
HapII	CCGG	2 cut(s)	162, 2081
HgaI	GACGC	3 cut(s)	147, 2438, 2906
HhaI	GCGC	1 cut(s)	30
Hin1I	GRCGYC	1 cut(s)	2917
Hin6I	GCGC	1 cut(s)	28
HinP1I	GCGC	1 cut(s)	28
HindIII	AAGCTT	2 cut(s)	188, 2281
HpaII	CCGG	2 cut(s)	162, 2081
HphI	GGTGA	7 cut(s)	172, 854, 1490, 2060, 2575, 2672, 2728
Hpy166II	GTNNAC	4 cut(s)	845, 2051, 2614, 3224
Hpy8I	GTNNAC	4 cut(s)	845, 2051, 2614, 3224
HpyCH4III	ACNGT	5 cut(s)	491, 637, 1263, 2491, 2974
HpyCH4IV	ACGT	3 cut(s)	1425, 1765, 2872
HpyF10VI	GCNNNNNNNGC	7 cut(s)	128, 1927, 2069, 2539, 2545, 3005, 3230
HpySE526I	ACGT	3 cut(s)	1425, 1765, 2872
Hsp92I	GRCGYC	1 cut(s)	2917
HspAI	GCGC	1 cut(s)	28
Kpn2I	TCCGGA	2 cut(s)	161, 2080
Ksp22I	TGATCA	2 cut(s)	355, 2709
Lsp1109I	GCAGC	5 cut(s)	425, 1517, 1831, 2526, 2983
LweI	GCATC	6 cut(s)	1021, 1750, 2099, 2265, 2693, 3658
MaeI	CTAG	7 cut(s)	525, 648, 1313, 1611, 2192, 3015, 3446
MaeII	ACGT	3 cut(s)	1425, 1765, 2872
MaeIII	GTNAC	4 cut(s)	323, 527, 2716, 2731
MfeI	CAATTG	3 cut(s)	2625, 2746, 3234
MflI	RGATCY	7 cut(s)	223, 956, 976, 1831, 3076, 3127, 3499
MhlI	GDGCHC	6 cut(s)	496, 1191, 1474, 2867, 3031, 3226
MlsI	TGGCCA	3 cut(s)	1287, 1799, 1930
MluNI	TGGCCA	3 cut(s)	1287, 1799, 1930
MlyI	GAGTC	4 cut(s)	320, 914, 1643, 2823
MmeI	TCCRAC	6 cut(s)	575, 798, 897, 1794, 2055, 2436
Mox20I	TGGCCA	3 cut(s)	1287, 1799, 1930
Mph1103I	ATGCAT	2 cut(s)	675, 2164
MroI	TCCGGA	2 cut(s)	161, 2080
MscI	TGGCCA	3 cut(s)	1287, 1799, 1930
MslI	CAYNNNNRTG	3 cut(s)	1595, 1707, 2304
Msp20I	TGGCCA	3 cut(s)	1287, 1799, 1930
MspA1I	CMGCKG	1 cut(s)	1822
MspCI	CTTAAG	1 cut(s)	1349
MspI	CCGG	2 cut(s)	162, 2081
MspR9I	CCNGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
MunI	CAATTG	3 cut(s)	2625, 2746, 3234
Mva1269I	GAATGC	2 cut(s)	2260, 3343
MvaI	CCWGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
MvnI	CGCG	1 cut(s)	30
MwoI	GCNNNNNNNGC	7 cut(s)	128, 1927, 2069, 2539, 2545, 3005, 3230
NcoI	CCATGG	1 cut(s)	2506
NdeI	CATATG	2 cut(s)	1155, 3267
NlaIV	GGNNCC	9 cut(s)	462, 540, 661, 958, 1991, 2866, 3028, 3275, 3313
NmeAIII	GCCGAG	1 cut(s)	106
NmuCI	GTSAC	2 cut(s)	323, 2716
NsiI	ATGCAT	2 cut(s)	675, 2164
NspI	RCATGY	3 cut(s)	2162, 2636, 3119
NspV	TTCGAA	2 cut(s)	14, 2570
PaeI	GCATGC	1 cut(s)	2162
PagI	TCATGA	1 cut(s)	2302
PceI	AGGCCT	3 cut(s)	196, 1445, 1695
PciI	ACATGT	1 cut(s)	3115
PctI	GAATGC	2 cut(s)	2260, 3343
PfeI	GAWTC	9 cut(s)	679, 800, 878, 1574, 1719, 2180, 2344, 2363, 2572
PfoI	TCCNGGA	1 cut(s)	3202
PkrI	GCNGC	6 cut(s)	415, 1041, 1507, 1821, 2541, 2998
PleI	GAGTC	4 cut(s)	320, 914, 1642, 2822
PpsI	GAGTC	4 cut(s)	320, 914, 1642, 2822
Ppu21I	YACGTR	1 cut(s)	1766
PscI	ACATGT	1 cut(s)	3115
PsiI	TTATAA	1 cut(s)	2153
Psp124BI	GAGCTC	1 cut(s)	1191
Psp6I	CCWGG	7 cut(s)	262, 1445, 1623, 1695, 3174, 3202, 3298
PspGI	CCWGG	7 cut(s)	262, 1445, 1623, 1695, 3174, 3202, 3298
PspN4I	GGNNCC	9 cut(s)	462, 540, 661, 958, 1991, 2866, 3028, 3275, 3313
PspPI	GGNCC	9 cut(s)	461, 539, 660, 740, 1019, 1208, 3274, 3312, 3601
PsrI	GAACNNNNNNTAC	2 cut(s)	2925, 2957
PstI	CTGCAG	4 cut(s)	1507, 1849, 2658, 3001
PstNI	CAGNNNCTG	1 cut(s)	3572
PsuI	RGATCY	7 cut(s)	223, 956, 976, 1831, 3076, 3127, 3499
PvuII	CAGCTG	1 cut(s)	1822
RsaI	GTAC	9 cut(s)	747, 1216, 1431, 1534, 1589, 1604, 1876, 1891, 1958
RsaNI	GTAC	9 cut(s)	746, 1215, 1430, 1533, 1588, 1603, 1875, 1890, 1957
RseI	CAYNNNNRTG	3 cut(s)	1595, 1707, 2304
SacI	GAGCTC	1 cut(s)	1191
SatI	GCNGC	6 cut(s)	414, 1040, 1506, 1820, 2540, 2997
Sau96I	GGNCC	9 cut(s)	461, 539, 660, 740, 1019, 1208, 3274, 3312, 3601
SbfI	CCTGCAGG	1 cut(s)	2658
ScaI	AGTACT	2 cut(s)	747, 1604
SchI	GAGTC	4 cut(s)	320, 914, 1643, 2823
ScrFI	CCNGG	7 cut(s)	264, 1447, 1625, 1697, 3176, 3204, 3300
SdaI	CCTGCAGG	1 cut(s)	2658
SduI	GDGCHC	6 cut(s)	496, 1191, 1474, 2867, 3031, 3226
SfaNI	GCATC	6 cut(s)	1021, 1750, 2099, 2265, 2693, 3658
SfcI	CTRYAG	8 cut(s)	1259, 1503, 1845, 2142, 2614, 2654, 2997, 3082
SfuI	TTCGAA	2 cut(s)	14, 2570
SinI	GGWCC	5 cut(s)	461, 660, 1019, 3312, 3601
SmiMI	CAYNNNNRTG	3 cut(s)	1595, 1707, 2304
SmlI	CTYRAG	3 cut(s)	869, 1349, 3091
SmoI	CTYRAG	3 cut(s)	869, 1349, 3091
SphI	GCATGC	1 cut(s)	2162
Sse8387I	CCTGCAGG	1 cut(s)	2658
SseBI	AGGCCT	3 cut(s)	196, 1445, 1695
SsiI	CCGC	8 cut(s)	30, 1040, 1345, 1378, 1905, 2101, 2843, 3414
SspI	AATATT	2 cut(s)	135, 2200
SspMI	CTAG	7 cut(s)	525, 648, 1313, 1611, 2192, 3015, 3446
SstI	GAGCTC	1 cut(s)	1191
StuI	AGGCCT	3 cut(s)	196, 1445, 1695
StyD4I	CCNGG	7 cut(s)	262, 1445, 1623, 1695, 3174, 3202, 3298
StyI	CCWWGG	4 cut(s)	647, 939, 1610, 2506
TaaI	ACNGT	5 cut(s)	491, 637, 1263, 2491, 2974
TaiI	ACGT	3 cut(s)	1428, 1768, 2875
TaqI	TCGA	9 cut(s)	14, 317, 408, 1126, 1234, 1869, 1980, 2570, 2667
TatI	WGTACW	5 cut(s)	745, 1214, 1587, 1602, 1956
TauI	GCSGC	1 cut(s)	1042
TfiI	GAWTC	9 cut(s)	679, 800, 878, 1574, 1719, 2180, 2344, 2363, 2572
TscAI	CASTG	8 cut(s)	52, 124, 450, 496, 520, 577, 2494, 3577
TseFI	GTSAC	2 cut(s)	323, 2716
TseI	GCWGC	5 cut(s)	413, 1505, 1819, 2539, 2996
Tsp45I	GTSAC	2 cut(s)	323, 2716
TspGWI	ACGGA	7 cut(s)	497, 989, 2112, 2905, 3067, 3527, 3593
TspRI	CASTG	8 cut(s)	52, 124, 450, 496, 520, 577, 2494, 3577
Vha464I	CTTAAG	1 cut(s)	1349
VneI	GTGCAC	1 cut(s)	3222
VpaK11BI	GGWCC	5 cut(s)	461, 660, 1019, 3312, 3601
XapI	RAATTY	7 cut(s)	364, 422, 628, 899, 2075, 2601, 3645
XceI	RCATGY	3 cut(s)	2162, 2636, 3119
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2452
XmaJI	CCTAGG	2 cut(s)	647, 1610
XmiI	GTMKAC	1 cut(s)	2613
XspI	CTAG	7 cut(s)	525, 648, 1313, 1611, 2192, 3015, 3446
ZrmI	AGTACT	2 cut(s)	747, 1604
Zsp2I	ATGCAT	2 cut(s)	675, 2164

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.