Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010438

Physical Location & Seq

Genomic Coordinates Forward (+)

4720270 .. 4742970

Gene Structure 22701 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1040400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3750 bp

ATGGGGCAGAAGAAGAAGAAGCGTGGAGGAGGGAGTGGGAAGAAGAAGAAGGCCTTGAAAGATCATGGGGCTTATGTTGGTGATGATGATAATGAACTTCTTTCTGAAGAGATCACTGCCTTGTGTGCAATATTTCAAGACGACTGCAAAGTTATGTCTGGATCACATCCTCAAATCATTATTAAGATTAGGCCTCATTCTAAGGATATGGGTTATGAGGATCTAGATGTTTCTGCACTTCTTTCAGTTAGGTGCTTACCGGGGTATCCTAACAAGTGCCCAAAGTTGCAGATAACTCCAGAGAAAGGTTTATCAAAAACTGATACTGATAGACTGCTATCTCTTATTCATGATCAGGCAAATTCTAATGCTCGAGAAGGACGGGTAATGATTTTCAATTTGGTAGAGACTGCCCAGGAATTCCTATCTGAAATTGTGCCAGTGAGCCAATCACATGGACCTGTAATATGTCCGACTACGGATAGCAGTGCTCAATTGTTTCAGAAGGACATTGCAATTTTAAGTAACAAGAAGGGGCCTTTTGTTTATGGTTTCATAGACCTGTTCATTGGTTCTGGAGAGTCTTGGAATTGGGGTTTTGGAGTGGATGAAACCAGTGGGATAAATCCATCGGTACCATCCCACACGGATATAAAGCAAAGCCCATTACAAAAGGCCCATCCTAAAGTTATGCGTGATATTCAGGAAAAGAAACTGGATAAGCATGCAGAGCAGACAAGTTTACAAGATGTAAAGCAAAGCCCATTACCTTCCCCTACGGTCCAGTTAGATACCCTTGGAGAAGATAGTGAAGATGGCAACAAGAGCATGTATTTTACAGATTCAAGTAGATTGTTGTTGGAGGAATCTGTAGAAAATGGTGGTAAAGCTGAGAAAGAGAATTTGGTCGTTAAGGATGATTCAACAGAAGATGAGTGGGAATCTGGAAGTGAGCAATCAGAGTCACTCTCATTTGCTTCCTTGGGTCATGATCAAGTGTCTCAAGATGTTACAAAGGATCTCATAATGGTTCATCTACTCCGTCTTGCCTGCTCTTCGAAAGGACCATTGGCTGATGCCTTGCCACAAATAACCACAGAATTGGAGAAGTTAGGAATTTTATCAGAATGGGCAAAGGAGTTAACTTCTAAACCACCTTCAGTTTTGAACAGAATATTTGATCATGCTTTCAGACAACATATGGTTGCATCCCAAATCTCTCAATTTTGGGAACCCGCGTCTGATTTCGATGGGCCAAGTACATCTCTCCCAAGCTCTCGGTATCTTAATGACTTTGAGGAGCTACAGTCCCTTGGTCATGGTGGCTTTGGCCATGTTGTATTGTGCAAAAATAAGCTAGATGGAAGGCAGTATGCAGTGAAGAAAATTCGCCTCAAGGATAAAAGCCTTCCTGTTAATGATCGAATATTGAGGGAAGTAGCTACACTTTCTCGTTTGCAGCATCAACATGTTGTACGATACTATCAGGCGTGGTTTGAAACAGGGATTATTGGTGTTCATGGTGATACCATGTGGGGTTCAATGACTGCAGCCAGCACCACTTTCAGCTTCAAAGGTACAAACTCGGCAGATGCTCTTGGGAATGAGAATAAGCTTGAATCGACTTATCTGTACATTCAAATGGAATATTGCCCAAGGACTTTACGCCAAATTTTCGAGTCATATAGTCGTGTTGACAAAGAATTAGCATGGCATTTGTGTCGTCAAATTGTTGAAGGCTTGGCACATATACATGGACAAGGCATCATTCATCGGGACTTAACCCCAAGCAACATATTCTTTGATGCGCGTAATGATATTAAAATTGGTGATTTTGGTCTGGCCAAGTTTTTTAAGTTGGAGCAGTTGGACCAAGAACCAAGTTTTCCTCCAGATACAGCCGGAGTTTCTCTTGATGGTACTGGCCAAGTTGGTACATATTTTTACACTGCACCAGAAATTGAGCAAGGATGGCCAAAGATAGATGAAAAGGCTGATATGTACAGCTTGGGAGTTGTATTCTTTGAGCTATGGCACCCATTTGGGACTGCAATGGAACGACACCATGTCCTATCTGACTTGAAACAGAAGGGCGAACTTCCTCCTGCTTGGGTTGCTGAATTTCCTGAACAGGCATCTCTGTTGCGGCGTTTAATGTCTCCAAGTCCATCAGATCGTCCTTCTGCCACTGAACTTCTAAAGCATGCATTTCCACCACGAATGGAGTCTGAGTTGTTAGACAATATTCTACGAACAATGCAAACCTCAGAGGACAGAAGTGTGTATGACAGAGTTCTGAATGCCATCTTTGATGAGGAGATGTTAAGCGTGAAAGATCAGCAACATCGTGATGGAAGTGTGGGATTAGGTGGAAGGGATACTTCTGCTGTGCAGTATGCAGATTTACAAACCGAGGCTCGTGATTACGTTGTTGACATCACAAGGGAGGTGTTCAGACAGCATTGTGCAAAACACCTTGAAGTAATACCTATGCATTTGCTAGATGATTGCCAACAGTTCAATAGGAACACTGTCAAACTTTTAACCCACGGAGGAGATATGCTTGAACTTTGTCATGAGCTGCGCTTGCCTTTTGTTAGTTGGGCGATCTCCAGCCAGAAATCTTCATTCAAACGTTACGAAATATCCTCTGTGTACAGAAGACCTGTTGGACATTCACCCCCAAGTCGCTACCTGCAGGGTGACTTTGATATTATTGGAGGCGCATCAGCATTAACAGAGGCAGAAGTCATCAAGGTGACAAGGGACATTATAGCTAGTTTTTTTCATTCAGTTTTTTGTGATATTCATCTAAACCATGGGGACTTACTGGAGGCAATTTGGGCTTGGGTAGGAGTCAAGGCAGAGCATAGGCAGAAAGTAGCAGAGCTTCTGTCTATGATGGGCTCTTTACGTCCTCAATCTTCTGAGCGGAAATCCAAATGGGTGGTAATCAGGCGTCAGCTTTTGCAGGAACTTAATTTACCGCAAGCTGTTGTAAATAGGTTGCAAACTGTTGGTTTACGGTTTTGTGGACCTGCAGATCAGGCACTTGCTAGACTGAGGGGAGCCTTGCCAACTGATAAACAAACACGGAAGGCACTTGATGAGCTGTCGGATCTCTACAGCCACTTGAGAGTTTGGAGGATCGAACGAAATGTTTACGTAGATGCACTTATGCCACCAACTGAGAGTTATCATAGAGATTTGTTCTTCCAGGTATATTTAGTGAAGGACAACAATCCTGGATCACTTGCCGAAGGTGCACTGCTTGCTGTTGGCGGGCGCTATGACTATTTACTTCGTCAAATGTGGGGTCTTGAACATAAATCAAATCCTCCGGGTGCTGTTGGGACCAGCCTTGCATTAGAGACAATAATTCAGCTTTCTTCTATAGATTTTAAACCAATCAGAAATGAAGTTAGCAACATTGTTCTTGTTTGTTCAAAAGGAGGAGGTGGTCTATTGAAAGAGCGCATGGAACTAGTCGCCGAGCTATGGGAGGAAAACATTAAGGCTGAGTTTGTCCCAACACCTGATCCAAGTCTTACAGAGCAGTATGAATATGCAAATGAACATGACATAAAGTGCCTTGTCATCATAACAGACACAGGTGTTTCACAGAAAGGCTCTGTTAAGGTCCGTCATCTTGAACTCAAAAAAGAAAAGGAAGTTGACAGAGAAGATCTTGTCAGGTTTCTGCTGGATGCAATGGCGATACAATTTAGAAATCCTTCCATTTGGACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0014070 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034198 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0046677 GO:0050896 GO:0051716 GO:0060992 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928

KEGG Pathways

Metabolic & Signaling

ko04138 ko04140 ko04141 ko05160 ko05162 ko05164 ko05168 ko05169 map04138 map04140 map04141 map05160 map05162 map05164 map05168 map05169

Pfam Domains

Protein Families

HGTP_anticodon2 Pkinase RWD tRNA-synt_His

Protein Analysis

1250

Amino Acids

140.49

Weight (kDa)

5.74

Isoelectric Point (pI)

43.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWD	PF05773	32 - 142	4e-19	RWD domain
Pkinase	PF00069	432 - 737	8e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	435 - 734	3.2e-23	Protein tyrosine and serine/threonine kinase
tRNA-synt_His	PF13393	887 - 1126	6.5e-11	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	1145 - 1239	1.4e-10	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	1145 - 1235	1.5e-08	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59410 AT3G59410 AT3G59410 AT3G59410 AT3G59410
fragaria_vesca	FvH4_4g10800 FvH4_4g10800
malus_domestica	MD04G1040400.v1.1
prunus_persica	Prupe.1G194100_v2.0.a1 Prupe.1G194100_v2.0.a1
pyrus_communis	pycom04g03520
rosa_chinensis	RchiOBHm_Chr4g0406781
rosa_laevigata	RLG00000008759
rosa_multiflora	Rmu_sc0006408.1_g000006
rosa_roxburghii	Rroxscaffold_5G00351540
rosa_rugosa	Rorug04G0072900
rosa_samantha	Rh4AG140400 Rh4BG137400 Rh4CG147300 Rh4DG134400
rosa_wichuraiana	Rw4G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	2703, 3046
Acc65I	GGTACC	1 cut(s)	634
AccB1I	GGYRCC	2 cut(s)	634, 2034
AccBSI	CCGCTC	1 cut(s)	2932
AccII	CGCG	2 cut(s)	1238, 1810
AciI	CCGC	5 cut(s)	1236, 2146, 2932, 2987, 3282
AclI	AACGTT	1 cut(s)	2635
AclWI	GGATC	7 cut(s)	169, 228, 1026, 3126, 3155, 3256, 3533
AcoI	YGGCCR	4 cut(s)	1330, 1842, 1924, 1973
AcsI	RAATTY	7 cut(s)	361, 419, 901, 1116, 1386, 1671, 2120
AcuI	CTGAAG	2 cut(s)	126, 1143
AcyI	GRCGYC	1 cut(s)	2959
AfaI	GTAC	9 cut(s)	636, 1261, 1476, 1579, 1634, 1921, 1936, 2003, 2657
AfiI	CCNNNNNNNGG	2 cut(s)	305, 3498
AflIII	ACRYGT	1 cut(s)	1468
AhdI	GACNNNNNGTC	1 cut(s)	2066
AhlI	ACTAGT	1 cut(s)	3484
AjnI	CCWGG	3 cut(s)	414, 3216, 3244
AjuI	GAANNNNNNNTTGG	4 cut(s)	887, 919, 1052, 1084
Alw21I	GWGCWC	2 cut(s)	493, 3268
Alw26I	GTCTC	4 cut(s)	401, 1005, 2163, 3365
Alw44I	GTGCAC	1 cut(s)	3264
AlwI	GGATC	7 cut(s)	169, 228, 1026, 3126, 3155, 3256, 3533
Ama87I	CYCGRG	1 cut(s)	372
AoxI	GGCC	9 cut(s)	51, 191, 536, 675, 1253, 1330, 1842, 1924, 1973
ApaLI	GTGCAC	1 cut(s)	3264
ApeKI	GCWGC	3 cut(s)	1459, 1550, 2581
ApoI	RAATTY	7 cut(s)	361, 419, 901, 1116, 1386, 1671, 2120
ArsI	GACNNNNNNTTYG	2 cut(s)	3301, 3333
Asp700I	GAANNNNTTC	1 cut(s)	3733
Asp718I	GGTACC	1 cut(s)	634
AspLEI	GCGC	5 cut(s)	1810, 2586, 2726, 3288, 3477
AsuC2I	CCSGG	2 cut(s)	261, 3342
AsuHPI	GGTGA	6 cut(s)	92, 1535, 1841, 2670, 2714, 2770
AsuII	TTCGAA	1 cut(s)	1058
AvaI	CYCGRG	1 cut(s)	372
AvaII	GGWCC	7 cut(s)	458, 781, 1064, 1870, 3035, 3354, 3640
BaeGI	GKGCMC	2 cut(s)	281, 3268
BaeI	ACNNNNGTAYC	2 cut(s)	248, 281
BalI	TGGCCA	4 cut(s)	1332, 1844, 1926, 1975
BanI	GGYRCC	2 cut(s)	634, 2034
BanII	GRGCYC	1 cut(s)	2909
BarI	GAAGNNNNNNTAC	2 cut(s)	2874, 2906
BauI	CACGAG	1 cut(s)	2418
BbsI	GAAGAC	1 cut(s)	2668
Bbv12I	GWGCWC	2 cut(s)	493, 3268
BbvI	GCAGC	3 cut(s)	1471, 1562, 2568
BcgI	CGANNNNNNTGC	2 cut(s)	1703, 1737
BciT130I	CCWGG	3 cut(s)	416, 3218, 3246
BciVI	GTATCC	2 cut(s)	276, 2371
BclI	TGATCA	3 cut(s)	352, 991, 1180
BcnI	CCSGG	2 cut(s)	261, 3342
BcoDI	GTCTC	4 cut(s)	401, 1005, 2163, 3365
BcuI	ACTAGT	1 cut(s)	3484
BfaI	CTAG	6 cut(s)	224, 1358, 2501, 2778, 3057, 3485
BfmI	CTRYAG	7 cut(s)	870, 1304, 1548, 2696, 3039, 3124, 3393
BfoI	RGCGCY	1 cut(s)	3289
BfuAI	ACCTGC	2 cut(s)	2703, 3046
BfuI	GTATCC	2 cut(s)	276, 2371
BglII	AGATCT	1 cut(s)	3685
BisI	GCNGC	4 cut(s)	1460, 1551, 2147, 2582
BlsI	GCNGC	4 cut(s)	1461, 1552, 2148, 2583
Bme1390I	CCNGG	5 cut(s)	261, 416, 3218, 3246, 3342
Bme18I	GGWCC	7 cut(s)	458, 781, 1064, 1870, 3035, 3354, 3640
BmeRI	GACNNNNNGTC	1 cut(s)	2066
BmeT110I	CYCGRG	1 cut(s)	372
BmiI	GGNNCC	6 cut(s)	537, 636, 1233, 2036, 3070, 3355
BmrFI	CCNGG	5 cut(s)	261, 416, 3218, 3246, 3342
BpiI	GAAGAC	1 cut(s)	2668
BplI	GAGNNNNNCTC	2 cut(s)	953, 985
BpmI	CTGGAG	5 cut(s)	282, 597, 1875, 2596, 2852
Bpu14I	TTCGAA	1 cut(s)	1058
BpuEI	CTTGAG	3 cut(s)	987, 1379, 3154
BpuMI	CCSGG	2 cut(s)	261, 3342
BsaAI	YACGTR	1 cut(s)	3166
BsaBI	GATNNNNATC	1 cut(s)	2808
BsaHI	GRCGYC	1 cut(s)	2959
BsaJI	CCNNGG	9 cut(s)	260, 414, 796, 981, 1312, 1655, 2412, 2548, 2818
BsaXI	ACNNNNNCTCC	4 cut(s)	1292, 1322, 2216, 2246
Bsc4I	CCNNNNNNNGG	2 cut(s)	305, 3498
Bse1I	ACTGG	6 cut(s)	440, 615, 720, 784, 1927, 2835
Bse3DI	GCAATG	3 cut(s)	510, 2058, 3717
Bse8I	GATNNNNATC	1 cut(s)	2808
BseBI	CCWGG	3 cut(s)	416, 3218, 3246
BseDI	CCNNGG	9 cut(s)	260, 414, 796, 981, 1312, 1655, 2412, 2548, 2818
BseGI	GGATG	8 cut(s)	166, 613, 638, 679, 922, 1208, 1976, 3712
BseJI	GATNNNNATC	1 cut(s)	2808
BseLI	CCNNNNNNNGG	2 cut(s)	305, 3498
BseMI	GCAATG	3 cut(s)	510, 2058, 3717
BseMII	CTCAG	7 cut(s)	882, 2220, 2280, 2919, 3053, 3180, 3510
BseNI	ACTGG	6 cut(s)	440, 615, 720, 784, 1927, 2835
BseRI	GAGGAG	5 cut(s)	42, 1313, 2330, 2568, 3468
BseSI	GKGCMC	2 cut(s)	281, 3268
BseXI	GCAGC	3 cut(s)	1471, 1562, 2568
BsgI	GTGCAG	3 cut(s)	219, 1935, 2411
Bsh1236I	CGCG	2 cut(s)	1238, 1810
BshFI	GGCC	9 cut(s)	53, 193, 538, 677, 1255, 1332, 1844, 1926, 1975
BshNI	GGYRCC	2 cut(s)	634, 2034
BsiHKAI	GWGCWC	2 cut(s)	493, 3268
BsiHKCI	CYCGRG	1 cut(s)	372
BsiSI	CCGG	3 cut(s)	260, 1902, 3341
BslFI	GGGAC	7 cut(s)	1294, 1790, 2059, 2780, 2837, 3367, 3512
BslI	CCNNNNNNNGG	2 cut(s)	305, 3498
BsmAI	GTCTC	4 cut(s)	401, 1005, 2163, 3365
BsmFI	GGGAC	7 cut(s)	1294, 1790, 2059, 2780, 2837, 3367, 3512
BsmI	GAATGC	1 cut(s)	2305
BsnI	GGCC	9 cut(s)	53, 193, 538, 677, 1255, 1332, 1844, 1926, 1975
BsoBI	CYCGRG	1 cut(s)	372
Bsp119I	TTCGAA	1 cut(s)	1058
Bsp1286I	GDGCHC	4 cut(s)	281, 493, 2909, 3268
Bsp1407I	TGTACA	3 cut(s)	1632, 2001, 2655
Bsp19I	CCATGG	1 cut(s)	2818
BspACI	CCGC	5 cut(s)	1236, 2146, 2932, 2987, 3282
BspANI	GGCC	9 cut(s)	53, 193, 538, 677, 1255, 1332, 1844, 1926, 1975
BspCNI	CTCAG	7 cut(s)	883, 2221, 2279, 2920, 3054, 3181, 3511
BspFNI	CGCG	2 cut(s)	1238, 1810
BspHI	TCATGA	3 cut(s)	349, 988, 2575
BspLI	GGNNCC	6 cut(s)	537, 636, 1233, 2036, 3070, 3355
BspMAI	CTGCAG	3 cut(s)	1552, 2700, 3043
BspMI	ACCTGC	2 cut(s)	2703, 3046
BspPI	GGATC	7 cut(s)	169, 228, 1026, 3126, 3155, 3256, 3533
BspQI	GCTCTTC	1 cut(s)	1060
BspT104I	TTCGAA	1 cut(s)	1058
BspT107I	GGYRCC	2 cut(s)	634, 2034
BsrBI	CCGCTC	1 cut(s)	2932
BsrDI	GCAATG	3 cut(s)	510, 2058, 3717
BsrGI	TGTACA	3 cut(s)	1632, 2001, 2655
BsrI	ACTGG	6 cut(s)	440, 615, 720, 784, 1927, 2835
BssECI	CCNNGG	9 cut(s)	260, 414, 796, 981, 1312, 1655, 2412, 2548, 2818
BssNI	GRCGYC	1 cut(s)	2959
BssSI	CACGAG	1 cut(s)	2418
BssT1I	CCWWGG	5 cut(s)	796, 981, 1312, 1655, 2818
Bst2BI	CACGAG	1 cut(s)	2418
Bst2UI	CCWGG	3 cut(s)	416, 3218, 3246
Bst4CI	ACNGT	6 cut(s)	781, 1308, 2517, 2533, 3016, 3027
Bst6I	CTCTTC	2 cut(s)	102, 1060
BstACI	GRCGYC	1 cut(s)	2959
BstAPI	GCANNNNNTGC	1 cut(s)	3272
BstAUI	TGTACA	3 cut(s)	1632, 2001, 2655
BstBAI	YACGTR	1 cut(s)	3166
BstBI	TTCGAA	1 cut(s)	1058
BstC8I	GCNNGC	8 cut(s)	726, 1051, 1555, 2205, 2588, 2991, 3273, 3284
BstDEI	CTNAG	9 cut(s)	201, 891, 2229, 2266, 2928, 3062, 3189, 3519, 3747
BstDSI	CCRYGG	2 cut(s)	2548, 2818
BstF5I	GGATG	8 cut(s)	166, 613, 638, 679, 922, 1208, 1976, 3712
BstFNI	CGCG	2 cut(s)	1238, 1810
BstH2I	RGCGCY	1 cut(s)	3289
BstHHI	GCGC	5 cut(s)	1810, 2586, 2726, 3288, 3477
BstMAI	GTCTC	4 cut(s)	401, 1005, 2163, 3365
BstNI	CCWGG	3 cut(s)	416, 3218, 3246
BstNSI	RCATGY	4 cut(s)	728, 832, 1472, 2207
BstSCI	CCNGG	5 cut(s)	259, 414, 3216, 3244, 3340
BstSFI	CTRYAG	7 cut(s)	870, 1304, 1548, 2696, 3039, 3124, 3393
BstSLI	GKGCMC	2 cut(s)	281, 3268
BstSNI	TACGTA	1 cut(s)	3166
BstUI	CGCG	2 cut(s)	1238, 1810
BstV1I	GCAGC	3 cut(s)	1471, 1562, 2568
BstV2I	GAAGAC	1 cut(s)	2668
BstX2I	RGATCY	4 cut(s)	220, 1018, 3118, 3685
BstXI	CCANNNNNNTGG	3 cut(s)	455, 1102, 2947
BstYI	RGATCY	4 cut(s)	220, 1018, 3118, 3685
BsuI	GTATCC	2 cut(s)	276, 2371
BsuRI	GGCC	9 cut(s)	53, 193, 538, 677, 1255, 1332, 1844, 1926, 1975
BtgI	CCRYGG	2 cut(s)	2548, 2818
BtsCI	GGATG	8 cut(s)	166, 613, 638, 679, 922, 1208, 1976, 3712
BtsI	GCAGTG	5 cut(s)	114, 493, 1383, 1947, 3266
BtsIMutI	CAGTG	9 cut(s)	114, 447, 493, 622, 1383, 1947, 2187, 2529, 3266
BveI	ACCTGC	2 cut(s)	2703, 3046
Cac8I	GCNNGC	8 cut(s)	726, 1051, 1555, 2205, 2588, 2991, 3273, 3284
CciI	TCATGA	3 cut(s)	349, 988, 2575
CfoI	GCGC	5 cut(s)	1810, 2586, 2726, 3288, 3477
CseI	GACGC	2 cut(s)	1227, 2948
Csp6I	GTAC	9 cut(s)	635, 1260, 1475, 1578, 1633, 1920, 1935, 2002, 2656
CviQI	GTAC	9 cut(s)	635, 1260, 1475, 1578, 1633, 1920, 1935, 2002, 2656
DdeI	CTNAG	9 cut(s)	201, 891, 2229, 2266, 2928, 3062, 3189, 3519, 3747
DraI	TTTAAA	1 cut(s)	3403
DriI	GACNNNNNGTC	1 cut(s)	2066
EaeI	YGGCCR	4 cut(s)	1330, 1842, 1924, 1973
Eam1104I	CTCTTC	2 cut(s)	102, 1060
Eam1105I	GACNNNNNGTC	1 cut(s)	2066
EarI	CTCTTC	2 cut(s)	102, 1060
Eco105I	TACGTA	1 cut(s)	3166
Eco130I	CCWWGG	5 cut(s)	796, 981, 1312, 1655, 2818
Eco147I	AGGCCT	2 cut(s)	53, 193
Eco24I	GRGCYC	1 cut(s)	2909
Eco47I	GGWCC	7 cut(s)	458, 781, 1064, 1870, 3035, 3354, 3640
Eco57I	CTGAAG	2 cut(s)	126, 1143
Eco88I	CYCGRG	1 cut(s)	372
EcoO109I	RGGNCCY	1 cut(s)	536
EcoRI	GAATTC	1 cut(s)	419
EcoRII	CCWGG	3 cut(s)	414, 3216, 3244
EcoT14I	CCWWGG	5 cut(s)	796, 981, 1312, 1655, 2818
EcoT22I	ATGCAT	2 cut(s)	2209, 2496
EcoT38I	GRGCYC	1 cut(s)	2909
ErhI	CCWWGG	5 cut(s)	796, 981, 1312, 1655, 2818
FalI	AAGNNNNNCTT	4 cut(s)	38, 70, 2365, 2397
FaqI	GGGAC	7 cut(s)	1294, 1790, 2059, 2780, 2837, 3367, 3512
FauI	CCCGC	2 cut(s)	1243, 3275
FauNDI	CATATG	1 cut(s)	1200
FbaI	TGATCA	3 cut(s)	352, 991, 1180
Fnu4HI	GCNGC	4 cut(s)	1460, 1551, 2147, 2582
FokI	GGATG	8 cut(s)	153, 620, 625, 666, 929, 1195, 1983, 3719
FriOI	GRGCYC	1 cut(s)	2909
Fsp4HI	GCNGC	4 cut(s)	1460, 1551, 2147, 2582
FspBI	CTAG	6 cut(s)	224, 1358, 2501, 2778, 3057, 3485
GlaI	GCGC	5 cut(s)	1809, 2585, 2725, 3287, 3476
GluI	GCNGC	4 cut(s)	1460, 1551, 2147, 2582
GsuI	CTGGAG	5 cut(s)	282, 597, 1875, 2596, 2852
HaeII	RGCGCY	1 cut(s)	3289
HaeIII	GGCC	9 cut(s)	53, 193, 538, 677, 1255, 1332, 1844, 1926, 1975
HapII	CCGG	3 cut(s)	260, 1902, 3341
HgaI	GACGC	2 cut(s)	1227, 2948
HhaI	GCGC	5 cut(s)	1810, 2586, 2726, 3288, 3477
Hin1I	GRCGYC	1 cut(s)	2959
Hin6I	GCGC	5 cut(s)	1808, 2584, 2724, 3286, 3475
HinP1I	GCGC	5 cut(s)	1808, 2584, 2724, 3286, 3475
HincII	GTYRAC	4 cut(s)	1143, 1696, 2434, 3676
HindII	GTYRAC	4 cut(s)	1143, 1696, 2434, 3676
HindIII	AAGCTT	1 cut(s)	1613
HpaI	GTTAAC	1 cut(s)	1143
HpaII	CCGG	3 cut(s)	260, 1902, 3341
HphI	GGTGA	6 cut(s)	92, 1535, 1841, 2670, 2714, 2770
HpyCH4III	ACNGT	6 cut(s)	781, 1308, 2517, 2533, 3016, 3027
HpyCH4IV	ACGT	4 cut(s)	2427, 2635, 2914, 3165
HpyF3I	CTNAG	9 cut(s)	201, 891, 2229, 2266, 2928, 3062, 3189, 3519, 3747
HpySE526I	ACGT	4 cut(s)	2427, 2635, 2914, 3165
Hsp92I	GRCGYC	1 cut(s)	2959
HspAI	GCGC	5 cut(s)	1808, 2584, 2724, 3286, 3475
KpnI	GGTACC	1 cut(s)	638
Ksp22I	TGATCA	3 cut(s)	352, 991, 1180
KspAI	GTTAAC	1 cut(s)	1143
LguI	GCTCTTC	1 cut(s)	1060
LmnI	GCTCC	3 cut(s)	1300, 1861, 3068
Lsp1109I	GCAGC	3 cut(s)	1471, 1562, 2568
MaeI	CTAG	6 cut(s)	224, 1358, 2501, 2778, 3057, 3485
MaeII	ACGT	4 cut(s)	2427, 2635, 2914, 3165
MaeIII	GTNAC	6 cut(s)	524, 963, 1009, 2636, 2702, 2758
MbiI	CCGCTC	1 cut(s)	2932
MfeI	CAATTG	1 cut(s)	494
MflI	RGATCY	4 cut(s)	220, 1018, 3118, 3685
MhlI	GDGCHC	4 cut(s)	281, 493, 2909, 3268
MlsI	TGGCCA	4 cut(s)	1332, 1844, 1926, 1975
MluNI	TGGCCA	4 cut(s)	1332, 1844, 1926, 1975
MlyI	GAGTC	5 cut(s)	590, 971, 1688, 2234, 2865
MmeI	TCCRAC	6 cut(s)	497, 840, 1839, 1848, 2650, 3096
Mox20I	TGGCCA	4 cut(s)	1332, 1844, 1926, 1975
Mph1103I	ATGCAT	2 cut(s)	2209, 2496
MroXI	GAANNNNTTC	1 cut(s)	3733
MscI	TGGCCA	4 cut(s)	1332, 1844, 1926, 1975
MslI	CAYNNNNRTG	5 cut(s)	1467, 1640, 1752, 2349, 2756
Msp20I	TGGCCA	4 cut(s)	1332, 1844, 1926, 1975
MspI	CCGG	3 cut(s)	260, 1902, 3341
MspR9I	CCNGG	5 cut(s)	261, 416, 3218, 3246, 3342
MunI	CAATTG	1 cut(s)	494
Mva1269I	GAATGC	1 cut(s)	2305
MvaI	CCWGG	3 cut(s)	416, 3218, 3246
MvnI	CGCG	2 cut(s)	1238, 1810
NciI	CCSGG	2 cut(s)	261, 3342
NcoI	CCATGG	1 cut(s)	2818
NdeI	CATATG	1 cut(s)	1200
NlaIV	GGNNCC	6 cut(s)	537, 636, 1233, 2036, 3070, 3355
NmeAIII	GCCGAG	2 cut(s)	1565, 3517
NmuCI	GTSAC	3 cut(s)	963, 2702, 2758
NsiI	ATGCAT	2 cut(s)	2209, 2496
NspI	RCATGY	4 cut(s)	728, 832, 1472, 2207
NspV	TTCGAA	1 cut(s)	1058
PaeI	GCATGC	2 cut(s)	728, 2207
PaeR7I	CTCGAG	1 cut(s)	372
PagI	TCATGA	3 cut(s)	349, 988, 2575
PceI	AGGCCT	2 cut(s)	53, 193
PciI	ACATGT	1 cut(s)	1468
PciSI	GCTCTTC	1 cut(s)	1060
PctI	GAATGC	1 cut(s)	2305
PdmI	GAANNNNTTC	1 cut(s)	3733
PfeI	GAWTC	5 cut(s)	842, 866, 920, 941, 1619
PfoI	TCCNGGA	1 cut(s)	3244
PkrI	GCNGC	4 cut(s)	1461, 1552, 2148, 2583
PleI	GAGTC	5 cut(s)	589, 970, 1687, 2233, 2864
PpsI	GAGTC	5 cut(s)	589, 970, 1687, 2233, 2864
Ppu21I	YACGTR	1 cut(s)	3166
PscI	ACATGT	1 cut(s)	1468
Psp1406I	AACGTT	1 cut(s)	2635
Psp6I	CCWGG	3 cut(s)	414, 3216, 3244
PspGI	CCWGG	3 cut(s)	414, 3216, 3244
PspN4I	GGNNCC	6 cut(s)	537, 636, 1233, 2036, 3070, 3355
PsrI	GAACNNNNNNTAC	2 cut(s)	2967, 2999
PstI	CTGCAG	3 cut(s)	1552, 2700, 3043
PsuI	RGATCY	4 cut(s)	220, 1018, 3118, 3685
RsaI	GTAC	9 cut(s)	636, 1261, 1476, 1579, 1634, 1921, 1936, 2003, 2657
RsaNI	GTAC	9 cut(s)	635, 1260, 1475, 1578, 1633, 1920, 1935, 2002, 2656
RseI	CAYNNNNRTG	5 cut(s)	1467, 1640, 1752, 2349, 2756
SapI	GCTCTTC	1 cut(s)	1060
SatI	GCNGC	4 cut(s)	1460, 1551, 2147, 2582
SbfI	CCTGCAGG	1 cut(s)	2700
SchI	GAGTC	5 cut(s)	590, 971, 1688, 2234, 2865
ScrFI	CCNGG	5 cut(s)	261, 416, 3218, 3246, 3342
SdaI	CCTGCAGG	1 cut(s)	2700
SduI	GDGCHC	4 cut(s)	281, 493, 2909, 3268
SfcI	CTRYAG	7 cut(s)	870, 1304, 1548, 2696, 3039, 3124, 3393
Sfr274I	CTCGAG	1 cut(s)	372
SfuI	TTCGAA	1 cut(s)	1058
SinI	GGWCC	7 cut(s)	458, 781, 1064, 1870, 3035, 3354, 3640
SlaI	CTCGAG	1 cut(s)	372
SmiMI	CAYNNNNRTG	5 cut(s)	1467, 1640, 1752, 2349, 2756
SmlI	CTYRAG	4 cut(s)	372, 1002, 1394, 3133
SmoI	CTYRAG	4 cut(s)	372, 1002, 1394, 3133
SnaBI	TACGTA	1 cut(s)	3166
SpeI	ACTAGT	1 cut(s)	3484
SphI	GCATGC	2 cut(s)	728, 2207
Sse8387I	CCTGCAGG	1 cut(s)	2700
SseBI	AGGCCT	2 cut(s)	53, 193
SsiI	CCGC	5 cut(s)	1236, 2146, 2932, 2987, 3282
SspI	AATATT	5 cut(s)	132, 1176, 1428, 1649, 2245
SspMI	CTAG	6 cut(s)	224, 1358, 2501, 2778, 3057, 3485
StuI	AGGCCT	2 cut(s)	53, 193
StyD4I	CCNGG	5 cut(s)	259, 414, 3216, 3244, 3340
StyI	CCWWGG	5 cut(s)	796, 981, 1312, 1655, 2818
TaaI	ACNGT	6 cut(s)	781, 1308, 2517, 2533, 3016, 3027
TaiI	ACGT	4 cut(s)	2430, 2638, 2917, 3168
TaqI	TCGA	7 cut(s)	373, 1058, 1248, 1423, 1622, 1677, 3150
TatI	WGTACW	4 cut(s)	1259, 1632, 2001, 2655
TauI	GCSGC	1 cut(s)	2149
TfiI	GAWTC	5 cut(s)	842, 866, 920, 941, 1619
TscAI	CASTG	9 cut(s)	121, 447, 493, 622, 1383, 1954, 2194, 2536, 3273
TseFI	GTSAC	3 cut(s)	963, 2702, 2758
TseI	GCWGC	3 cut(s)	1459, 1550, 2581
Tsp45I	GTSAC	3 cut(s)	963, 2702, 2758
TspGWI	ACGGA	6 cut(s)	494, 662, 1031, 2565, 3109, 3632
TspRI	CASTG	9 cut(s)	121, 447, 493, 622, 1383, 1954, 2194, 2536, 3273
VneI	GTGCAC	1 cut(s)	3264
VpaK11BI	GGWCC	7 cut(s)	458, 781, 1064, 1870, 3035, 3354, 3640
XapI	RAATTY	7 cut(s)	361, 419, 901, 1116, 1386, 1671, 2120
XbaI	TCTAGA	1 cut(s)	223
XceI	RCATGY	4 cut(s)	728, 832, 1472, 2207
XhoI	CTCGAG	1 cut(s)	372
XmnI	GAANNNNTTC	1 cut(s)	3733
XspI	CTAG	6 cut(s)	224, 1358, 2501, 2778, 3057, 3485
Zsp2I	ATGCAT	2 cut(s)	2209, 2496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.