Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0010438

Physical Location & Seq

Genomic Coordinates Reverse (-)

35669850 .. 35687564

Gene Structure 17715 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG140400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3705 bp

ATGGGACATAGTTCGAAAAAGAAGAAGCGCGGAGGAGGCAGTGGGAAGAAGAAGAAGCCCTTGAAAGATCATGCCGGGGCTTACGATAATGACCTTCTTTCTGAAGAGATCACTGCTTTGTGTGCAATATTTCAAGATGACTGCAAAATTGCGTCCGGAGCACAACCTCAAATCACCATCAAGCTTAGGCCTCACTCAAAGGATATGGGTTATGAGGATCTGGATGTCTCTGCACTTCTTTTAGTTAGGTGCTTACCAGGGTATCCTTACAAGTGTCCCAAGTTGCAGATAACTCCAGAGAAAGGATTATCGAAAAGTGACTCTGATAAGCTATTATCTCTTATCAATGATCAGGCAAATTCCAATGCCCGAGAAGGACGGGTAATGATCTTCAATTTGGTCGAGGCAGCTCAGGAATTTCTATCTGAAATTGTGCCAGTGGGTCAATCACAGGGACCAGTAATATGTTCAACTATGGATAACAGTGCTCAGTTGTTTCAAAATGACACTGCAATTTCTAGTAACAAGAAGGGGCCTTTTGTTTATGGTTTCATAGACCTCTTCAGTGGCTCTGGAAAGTCTTGGAGTTGGAGTTTTGGAGTTGACGAGACTAAAGGGATAAATTCTACTGTCCCATTGCCTAGGGTGGATGGGTCCAAACTCATGCATGAGATTCAGGAAAAGAAACATGATAAGCAAGCAGAACCTTTAAAATCACAAGATATAAAGCAAGGGCCAATACTTTCCCCTAATGTCAAGTTAGAAACGCTTGAAGAAGAGAGTGAAGGCAGGGATTCAAGTAGATTTTTGTTGGAAGAAATGGACAGAAATGGTGGTGAAACTGAGAAAGAGAGTTCAGTTCCTGAGGAGGATTCAACAGAAGATGATTGGGAATTCGGAAGTCAGCAGTCGGACTCGCTCTCTTTTACTTCCTTGGCTCATGAGCAAGGATCCCAAAATGTTAAAAGGGATCTAATAATGGTTCATCTGCTCCGTCTCGCCTGCACTAAAGGACCATTGGCTGATGCTTTGCCACAGATAACCACAGAACTGCAAAACATAGGGGTCTTATCAGAATGGGCAAGGGATTTAGCTTCAAAACCAATTTCACTTTTAAATCGAACATTTAATCATGCCTTCGGACAACAGATGGTTTCATCCCGTATCTCTCAATTCTGGGAGCTCACTTCTGATTTAGAAGGTCCAAGTACATCTCTCCCAAGTTCTCGATATCTCAATGACTTTGAGGAGCTACAGTCTCTTGGTCATGGTGGCTTTGGCCATGTTGTGTTGTGCAAAAATAAGCTAGATGGAAGGCAGTATGCAGTAAAGAAAATCCGCCTTAAGGATAAAAGCCTGCCACTTGATGACCGCATATTGAGGGAAGTCGCTACACTTTCTCGTTTGCAACATCAACACGTTGTACGATACTATCAGGCCTGGTTTGAAACAGGAGGTGTTGGTGCTCATGGTGATACTACCTGGGGTTCAAGGACTGCAGCCAGCTCCATTTTTAGCTTTAAAGGTACAAGCTCAGCAGATGATCTTGGGAATGAGAATAAACTTGAATCAACATATTTGTACATTCAAATGGAGTATTGCCCTAGGACATTACGCCAGGTTTTTGAGTCATATAGTCAATTTGACAAAGAATTAGCATGGCATTTGTTTCGTCAAATTGTTGAAGGCCTGGCACACATACATGGACAAGGAATCATCCATCGGGACTTAACGCCAAATAACATATTCTTTGATGCACGTAATGATATTAAAATTGGAGATTTTGGCTTGGCCAAGTTTTTGAAGTTGGAGCAGCTGGATCAAGATCCAAGTTTTCCTGCAGATACAACTGGAGTTTCTCTCGATGGTACTGGTCAAGTTGGTACATATTTTTACACCGCACCTGAAATTGAGCAAGGCTGGCCAAAGATTGATGAAAAGGCTGATATGTACAGCTTGGGAATTGTATTTTTCGAGCTATGGCACCCATTTGGGACTACAATGGAACGGCACCTTGTCCTATCCGACTTGAAACAGAAGGGTGAACTGCCTTCTGCTTGGGTTGCTGAATTTCCAGAACAAGCATCCTTGTTGCGGAGCTTAATGTCCCCAAGTCCATCAGATCGCCCTTCTGCTACAGAACTTATAAAGCATGCATTTCCACCACGAATGGAATCTGAGTTGCTAGACAATATTCTACGAACAATGCAAACCTCTGAGGACAGAAGTGTGTATGATAAAGTTCTGAATGCTATATTTGATGAAGAGATGCTAAGCTTGAAAGACCAGCAACATCATGATGGTAGGTTGAGGTTGGCTGGTAGTGATACTTCTGCGATTCAGTATGCAGATTTAGATTCGGAGGCTCGTGATTATGTTGTTGAGATCACAAGGGAGGTGTTCAGACAACATTGTGCGAAGCACCTTGAAGTAATACCCATGCGTCTGTTGGATGATTGCCAACATTTTATGAGAAGGAACACTGTCAAACTTTTGACCCATGGAGGAGATATGCTTGAACTTTTGCATGAGCTGCGTTTGCCTTTTGTTAGTTGGGTGATTTCGAATCAGAAATCTTCATTCAAAAGATATGAAATTTCATGTGTCTACAGAAGACCAATTGGACATGCACCCCCAAGTCGCTACCTGCAGGGTGATTTCGACATTATTGGAGGTGCATCAGCATTGACAGAGGCAGAAGTGATCAAGGTGACAAGGGACATTGTAACCCGTTTTGTCAATTGGGAGTTCTGCGACATTCATCTAAACCACGGGGACTTACTGGAGGCAATTTGGTCTTGGGTAGGAGTCAAGGCAGACCATAGACAAAAAGTAGCGGAGCTTCTTTCTATGATGGGCTCCTTACGTCCTCAGTCTTCTGAACGGAAATCCAAATGGGTGGTAATAAGGCGTCAGCTTTTGCAGGAACTTAATTTACAAGAGGCTGTTGTGAATAGGTTGCAGACTGTTGGTTTAAGGTTCTGTGGAGCTGCAGATCAGTCACTTCATAGACTTAGGGGAGCCCTGCCAAACGATAAACCAACACGGAAGGCACTTGATGAGTTGTCAGATCTCTGTAGCTACTTGAGAGCTTGGAGGATTGAAGAACATGTTTATATAGATCCACTTATCCCCCCAACTGAGAGTTATCATAGAGACTTGTTTTTCCAGGTATATTTGGTGAAGGATAGTAATCCTGGATCAGTTACCGAAGGTGCACTACTTGCAATTGGGGGTCGGTATGACTATTTGCTTCATCATATGTGGGGTCTTGAACATAAATCTAATCCTCCTGGTTCTGTTGGGACCAGCCTTGCTTTAGAGACCATAATTCAGCATTCTCCTGTGGATTTTAAACCAATTAGCAAAAATGAAGTTAGCAATAGTGTTCTTGTTTGTTCAAAAGGAGGAGGGGGTCTATTGGCAGAACGCATGGAACTAGTCAATGAGCTTTGGGAAGAAAACATTAAGGCTGAGTTTCTCCCAACACCAGATCCAAGCCTTACGGAGCAGTATGAATATGCCAACGAACATGATATTAAATGTCTTGTCATCATAACAGACACTGGTGTTTCTCAGAAAGGTTCTGTTAAGGTCCGTCATCTTGAACTGAAGAAGGAAAAGGAGGTTGAAAGGGAAATTCTCGTCAGGTTTCTGCTGGATGCAATGGCGATACAATTTAAAAATCCTTCCATTTGGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0006950 GO:0006979 GO:0007154 GO:0008150 GO:0009267 GO:0009268 GO:0009605 GO:0009628 GO:0009636 GO:0009719 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010447 GO:0014070 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034198 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042594 GO:0046677 GO:0050896 GO:0051716 GO:0060992 GO:0070301 GO:0070887 GO:0071214 GO:0071236 GO:0071310 GO:0071407 GO:0071417 GO:0071467 GO:0071468 GO:0071495 GO:0071496 GO:0072755 GO:0097237 GO:0104004 GO:1901561 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1990451 GO:1990928

KEGG Pathways

Metabolic & Signaling

ko04138 ko04140 ko04141 ko05160 ko05162 ko05164 ko05168 ko05169 map04138 map04140 map04141 map05160 map05162 map05164 map05168 map05169

Pfam Domains

Protein Families

HGTP_anticodon2 Pkinase RWD tRNA-synt_His

Protein Analysis

1234

Amino Acids

139.22

Weight (kDa)

5.7

Isoelectric Point (pI)

42.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RWD	PF05773	32 - 141	1.6e-18	RWD domain
Pkinase	PF00069	415 - 720	7.1e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	524 - 717	3.8e-16	Protein tyrosine and serine/threonine kinase
tRNA-synt_His	PF13393	867 - 1109	1.4e-11	Histidyl-tRNA synthetase
HGTP_anticodon2	PF12745	1130 - 1224	3.2e-09	Anticodon binding domain of tRNAs
HGTP_anticodon	PF03129	1131 - 1220	2.2e-07	Anticodon binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010438)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59410 AT3G59410 AT3G59410 AT3G59410 AT3G59410
fragaria_vesca	FvH4_4g10800 FvH4_4g10800
malus_domestica	MD04G1040400.v1.1
prunus_persica	Prupe.1G194100_v2.0.a1 Prupe.1G194100_v2.0.a1
pyrus_communis	pycom04g03520
rosa_chinensis	RchiOBHm_Chr4g0406781
rosa_laevigata	RLG00000008759
rosa_multiflora	Rmu_sc0006408.1_g000006
rosa_roxburghii	Rroxscaffold_5G00351540
rosa_rugosa	Rorug04G0072900
rosa_samantha	Rh4AG140400 Rh4BG137400 Rh4CG147300 Rh4DG134400
rosa_wichuraiana	Rw4G011440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2147
Acc36I	ACCTGC	1 cut(s)	2655
AccB1I	GGYRCC	2 cut(s)	1983, 2010
AccI	GTMKAC	1 cut(s)	2607
AccII	CGCG	1 cut(s)	30
AccIII	TCCGGA	1 cut(s)	155
AciI	CCGC	6 cut(s)	30, 1339, 1372, 1899, 2095, 2837
AclWI	GGATC	9 cut(s)	225, 945, 958, 978, 1820, 1827, 3116, 3208, 3488
AcoI	YGGCCR	3 cut(s)	1279, 1791, 1922
AcsI	RAATTY	7 cut(s)	358, 416, 622, 893, 2069, 2595, 3639
AcuI	CTGAAG	3 cut(s)	123, 547, 3632
AcyI	GRCGYC	1 cut(s)	2911
AfaI	GTAC	7 cut(s)	1210, 1425, 1528, 1583, 1870, 1885, 1952
AfiI	CCNNNNNNNGG	3 cut(s)	302, 1345, 2094
AflII	CTTAAG	1 cut(s)	1343
AflIII	ACRYGT	2 cut(s)	1417, 3109
AhlI	ACTAGT	1 cut(s)	3439
AjnI	CCWGG	8 cut(s)	256, 1439, 1481, 1617, 1689, 3168, 3196, 3292
Alw21I	GWGCWC	5 cut(s)	163, 490, 1185, 1468, 3220
Alw26I	GTCTC	6 cut(s)	232, 602, 1001, 1263, 3150, 3317
Alw44I	GTGCAC	1 cut(s)	3216
AlwI	GGATC	9 cut(s)	225, 945, 958, 978, 1820, 1827, 3116, 3208, 3488
AlwNI	CAGNNNCTG	2 cut(s)	863, 3566
Ama87I	CYCGRG	1 cut(s)	369
Aor13HI	TCCGGA	1 cut(s)	155
AoxI	GGCC	8 cut(s)	188, 533, 734, 1279, 1437, 1687, 1791, 1922
ApaLI	GTGCAC	1 cut(s)	3216
ApeKI	GCWGC	5 cut(s)	407, 1499, 1813, 2533, 2990
ApoI	RAATTY	7 cut(s)	358, 416, 622, 893, 2069, 2595, 3639
AspA2I	CCTAGG	2 cut(s)	641, 1604
AspLEI	GCGC	1 cut(s)	30
AspS9I	GGNCC	8 cut(s)	455, 533, 654, 734, 1013, 1202, 3306, 3595
AsuC2I	CCSGG	1 cut(s)	76
AsuHPI	GGTGA	8 cut(s)	166, 848, 1484, 2054, 2569, 2666, 2722, 3193
AsuII	TTCGAA	2 cut(s)	14, 2564
AvaI	CYCGRG	1 cut(s)	369
AvaII	GGWCC	6 cut(s)	455, 654, 1013, 1202, 3306, 3595
AvrII	CCTAGG	2 cut(s)	641, 1604
AxyI	CCTNAGG	1 cut(s)	864
BaeGI	GKGCMC	1 cut(s)	3220
BaeI	ACNNNNGTAYC	2 cut(s)	245, 278
BalI	TGGCCA	3 cut(s)	1281, 1793, 1924
BamHI	GGATCC	1 cut(s)	950
BanI	GGYRCC	2 cut(s)	1983, 2010
BanII	GRGCYC	3 cut(s)	1185, 2861, 3025
BarI	GAAGNNNNNNTAC	6 cut(s)	2314, 2346, 2826, 2858, 3204, 3236
BauI	CACGAG	1 cut(s)	2367
BbsI	GAAGAC	2 cut(s)	2620, 2868
Bbv12I	GWGCWC	5 cut(s)	163, 490, 1185, 1468, 3220
BbvI	GCAGC	5 cut(s)	419, 1511, 1825, 2520, 2977
BccI	CCATC	9 cut(s)	185, 644, 1144, 1304, 1728, 1859, 2125, 2294, 2848
BceAI	ACGGC	1 cut(s)	2024
BcgI	CGANNNNNNTGC	2 cut(s)	1652, 1686
BciT130I	CCWGG	8 cut(s)	258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
BciVI	GTATCC	1 cut(s)	273
BclI	TGATCA	2 cut(s)	349, 2703
BcnI	CCSGG	1 cut(s)	76
BcoDI	GTCTC	6 cut(s)	232, 602, 1001, 1263, 3150, 3317
BcuI	ACTAGT	1 cut(s)	3439
BfaI	CTAG	6 cut(s)	519, 642, 1307, 1605, 2186, 3440
BfmI	CTRYAG	8 cut(s)	1253, 1497, 1839, 2136, 2608, 2648, 2991, 3076
BfrI	CTTAAG	1 cut(s)	1343
BfuAI	ACCTGC	1 cut(s)	2655
BfuI	GTATCC	1 cut(s)	273
BglII	AGATCT	1 cut(s)	3070
BisI	GCNGC	5 cut(s)	408, 1500, 1814, 2534, 2991
BlnI	CCTAGG	2 cut(s)	641, 1604
BlpI	GCTNAGC	2 cut(s)	1534, 2273
BlsI	GCNGC	5 cut(s)	409, 1501, 1815, 2535, 2992
Bme1390I	CCNGG	9 cut(s)	76, 258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
Bme18I	GGWCC	6 cut(s)	455, 654, 1013, 1202, 3306, 3595
BmeT110I	CYCGRG	1 cut(s)	369
BmgT120I	GGNCC	8 cut(s)	455, 533, 654, 734, 1013, 1202, 3306, 3595
BmiI	GGNNCC	9 cut(s)	456, 534, 655, 952, 1985, 2012, 2860, 3022, 3307
BmrFI	CCNGG	9 cut(s)	76, 258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
BmsI	GCATC	6 cut(s)	1015, 1744, 2093, 2259, 2687, 3652
BpiI	GAAGAC	2 cut(s)	2620, 2868
BplI	GAGNNNNNCTC	2 cut(s)	1845, 1877
BpmI	CTGGAG	3 cut(s)	279, 1872, 2804
Bpu10I	CCTNAGC	2 cut(s)	185, 411
Bpu1102I	GCTNAGC	2 cut(s)	1534, 2273
Bpu14I	TTCGAA	2 cut(s)	14, 2564
BpuEI	CTTGAG	1 cut(s)	3106
BpuMI	CCSGG	1 cut(s)	76
BsaAI	YACGTR	1 cut(s)	1760
BsaBI	GATNNNNATC	2 cut(s)	1824, 3192
BsaHI	GRCGYC	1 cut(s)	2911
BsaI	GGTCTC	1 cut(s)	3317
BsaJI	CCNNGG	8 cut(s)	75, 257, 641, 933, 1482, 1604, 2500, 2770
BsaWI	WCCGGW	1 cut(s)	155
BsaXI	ACNNNNNCTCC	4 cut(s)	577, 607, 1241, 1271
Bsc4I	CCNNNNNNNGG	3 cut(s)	302, 1345, 2094
Bse1I	ACTGG	6 cut(s)	437, 458, 1855, 1876, 2787, 3571
Bse21I	CCTNAGG	1 cut(s)	864
Bse3DI	GCAATG	2 cut(s)	635, 3672
Bse8I	GATNNNNATC	2 cut(s)	1824, 3192
BseAI	TCCGGA	1 cut(s)	155
BseBI	CCWGG	8 cut(s)	258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
BseDI	CCNNGG	8 cut(s)	75, 257, 641, 933, 1482, 1604, 2500, 2770
BseGI	GGATG	7 cut(s)	229, 655, 1157, 1716, 2084, 2458, 3667
BseJI	GATNNNNATC	2 cut(s)	1824, 3192
BseLI	CCNNNNNNNGG	3 cut(s)	302, 1345, 2094
BseMI	GCAATG	2 cut(s)	635, 3672
BseNI	ACTGG	6 cut(s)	437, 458, 1855, 1876, 2787, 3571
BseRI	GAGGAG	5 cut(s)	48, 881, 1262, 2520, 3423
BseSI	GKGCMC	1 cut(s)	3220
BseXI	GCAGC	5 cut(s)	419, 1511, 1825, 2520, 2977
BsgI	GTGCAG	2 cut(s)	216, 988
Bsh1236I	CGCG	1 cut(s)	30
BshFI	GGCC	8 cut(s)	190, 535, 736, 1281, 1439, 1689, 1793, 1924
BshNI	GGYRCC	2 cut(s)	1983, 2010
BsiHKAI	GWGCWC	5 cut(s)	163, 490, 1185, 1468, 3220
BsiHKCI	CYCGRG	1 cut(s)	369
BsiSI	CCGG	2 cut(s)	75, 156
BslI	CCNNNNNNNGG	3 cut(s)	302, 1345, 2094
BsmAI	GTCTC	6 cut(s)	232, 602, 1001, 1263, 3150, 3317
BsmBI	CGTCTC	1 cut(s)	1001
BsmI	GAATGC	2 cut(s)	2254, 3337
BsnI	GGCC	8 cut(s)	190, 535, 736, 1281, 1439, 1689, 1793, 1924
Bso31I	GGTCTC	1 cut(s)	3317
BsoBI	CYCGRG	1 cut(s)	369
Bsp119I	TTCGAA	2 cut(s)	14, 2564
Bsp1286I	GDGCHC	7 cut(s)	163, 490, 1185, 1468, 2861, 3025, 3220
Bsp13I	TCCGGA	1 cut(s)	155
Bsp1407I	TGTACA	2 cut(s)	1581, 1950
Bsp1720I	GCTNAGC	2 cut(s)	1534, 2273
Bsp19I	CCATGG	1 cut(s)	2500
BspACI	CCGC	6 cut(s)	30, 1339, 1372, 1899, 2095, 2837
BspANI	GGCC	8 cut(s)	190, 535, 736, 1281, 1439, 1689, 1793, 1924
BspEI	TCCGGA	1 cut(s)	155
BspFNI	CGCG	1 cut(s)	30
BspHI	TCATGA	2 cut(s)	940, 2296
BspLI	GGNNCC	9 cut(s)	456, 534, 655, 952, 1985, 2012, 2860, 3022, 3307
BspMAI	CTGCAG	4 cut(s)	1501, 1843, 2652, 2995
BspMI	ACCTGC	1 cut(s)	2655
BspPI	GGATC	9 cut(s)	225, 945, 958, 978, 1820, 1827, 3116, 3208, 3488
BspT104I	TTCGAA	2 cut(s)	14, 2564
BspT107I	GGYRCC	2 cut(s)	1983, 2010
BspTI	CTTAAG	1 cut(s)	1343
BspTNI	GGTCTC	1 cut(s)	3317
BsrDI	GCAATG	2 cut(s)	635, 3672
BsrGI	TGTACA	2 cut(s)	1581, 1950
BsrI	ACTGG	6 cut(s)	437, 458, 1855, 1876, 2787, 3571
BssECI	CCNNGG	8 cut(s)	75, 257, 641, 933, 1482, 1604, 2500, 2770
BssNI	GRCGYC	1 cut(s)	2911
BssSI	CACGAG	1 cut(s)	2367
BssT1I	CCWWGG	4 cut(s)	641, 933, 1604, 2500
Bst2BI	CACGAG	1 cut(s)	2367
Bst2UI	CCWGG	8 cut(s)	258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
Bst4CI	ACNGT	5 cut(s)	485, 631, 1257, 2485, 2968
Bst6I	CTCTTC	4 cut(s)	99, 566, 771, 2259
BstACI	GRCGYC	1 cut(s)	2911
BstAFI	CTTAAG	1 cut(s)	1343
BstAPI	GCANNNNNTGC	2 cut(s)	2533, 3224
BstAUI	TGTACA	2 cut(s)	1581, 1950
BstBAI	YACGTR	1 cut(s)	1760
BstBI	TTCGAA	2 cut(s)	14, 2564
BstC8I	GCNNGC	6 cut(s)	699, 1003, 1358, 1504, 1922, 2154
BstDSI	CCRYGG	2 cut(s)	2500, 2770
BstF5I	GGATG	7 cut(s)	229, 655, 1157, 1716, 2084, 2458, 3667
BstFNI	CGCG	1 cut(s)	30
BstHHI	GCGC	1 cut(s)	30
BstMAI	GTCTC	6 cut(s)	232, 602, 1001, 1263, 3150, 3317
BstMWI	GCNNNNNNNGC	8 cut(s)	36, 122, 158, 1921, 2063, 2533, 2539, 3224
BstNI	CCWGG	8 cut(s)	258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
BstNSI	RCATGY	3 cut(s)	2156, 2630, 3113
BstSCI	CCNGG	9 cut(s)	74, 256, 1439, 1481, 1617, 1689, 3168, 3196, 3292
BstSFI	CTRYAG	8 cut(s)	1253, 1497, 1839, 2136, 2608, 2648, 2991, 3076
BstSLI	GKGCMC	1 cut(s)	3220
BstUI	CGCG	1 cut(s)	30
BstV1I	GCAGC	5 cut(s)	419, 1511, 1825, 2520, 2977
BstV2I	GAAGAC	2 cut(s)	2620, 2868
BstX2I	RGATCY	7 cut(s)	217, 950, 970, 1825, 3070, 3121, 3493
BstXI	CCANNNNNNTGG	1 cut(s)	2899
BstYI	RGATCY	7 cut(s)	217, 950, 970, 1825, 3070, 3121, 3493
Bsu36I	CCTNAGG	1 cut(s)	864
BsuI	GTATCC	1 cut(s)	273
BsuRI	GGCC	8 cut(s)	190, 535, 736, 1281, 1439, 1689, 1793, 1924
BtgI	CCRYGG	2 cut(s)	2500, 2770
BtsCI	GGATG	7 cut(s)	229, 655, 1157, 1716, 2084, 2458, 3667
BtsI	GCAGTG	3 cut(s)	46, 111, 507
BtsIMutI	CAGTG	8 cut(s)	46, 111, 444, 490, 507, 571, 2481, 3564
BveI	ACCTGC	1 cut(s)	2655
Cac8I	GCNNGC	6 cut(s)	699, 1003, 1358, 1504, 1922, 2154
CaiI	CAGNNNCTG	2 cut(s)	863, 3566
CciI	TCATGA	2 cut(s)	940, 2296
CfoI	GCGC	1 cut(s)	30
Cfr13I	GGNCC	8 cut(s)	455, 533, 654, 734, 1013, 1202, 3306, 3595
CseI	GACGC	3 cut(s)	141, 2432, 2900
Csp6I	GTAC	7 cut(s)	1209, 1424, 1527, 1582, 1869, 1884, 1951
CviQI	GTAC	7 cut(s)	1209, 1424, 1527, 1582, 1869, 1884, 1951
DraI	TTTAAA	5 cut(s)	711, 1116, 1522, 3355, 3682
EaeI	YGGCCR	3 cut(s)	1279, 1791, 1922
Eam1104I	CTCTTC	4 cut(s)	99, 566, 771, 2259
EarI	CTCTTC	4 cut(s)	99, 566, 771, 2259
EciI	GGCGGA	1 cut(s)	1328
Ecl136II	GAGCTC	1 cut(s)	1183
Eco130I	CCWWGG	4 cut(s)	641, 933, 1604, 2500
Eco147I	AGGCCT	3 cut(s)	190, 1439, 1689
Eco24I	GRGCYC	3 cut(s)	1185, 2861, 3025
Eco31I	GGTCTC	1 cut(s)	3317
Eco32I	GATATC	1 cut(s)	1232
Eco47I	GGWCC	6 cut(s)	455, 654, 1013, 1202, 3306, 3595
Eco53kI	GAGCTC	1 cut(s)	1183
Eco57I	CTGAAG	3 cut(s)	123, 547, 3632
Eco81I	CCTNAGG	1 cut(s)	864
Eco88I	CYCGRG	1 cut(s)	369
EcoICRI	GAGCTC	1 cut(s)	1183
EcoO109I	RGGNCCY	1 cut(s)	533
EcoRI	GAATTC	1 cut(s)	893
EcoRII	CCWGG	8 cut(s)	256, 1439, 1481, 1617, 1689, 3168, 3196, 3292
EcoRV	GATATC	1 cut(s)	1232
EcoT14I	CCWWGG	4 cut(s)	641, 933, 1604, 2500
EcoT22I	ATGCAT	2 cut(s)	669, 2158
EcoT38I	GRGCYC	3 cut(s)	1185, 2861, 3025
ErhI	CCWWGG	4 cut(s)	641, 933, 1604, 2500
Esp3I	CGTCTC	1 cut(s)	1001
FalI	AAGNNNNNCTT	2 cut(s)	44, 76
FauNDI	CATATG	1 cut(s)	3261
FbaI	TGATCA	2 cut(s)	349, 2703
FblI	GTMKAC	1 cut(s)	2607
Fnu4HI	GCNGC	5 cut(s)	408, 1500, 1814, 2534, 2991
FokI	GGATG	7 cut(s)	236, 662, 1144, 1703, 2071, 2465, 3674
FriOI	GRGCYC	3 cut(s)	1185, 2861, 3025
Fsp4HI	GCNGC	5 cut(s)	408, 1500, 1814, 2534, 2991
FspBI	CTAG	6 cut(s)	519, 642, 1307, 1605, 2186, 3440
GlaI	GCGC	1 cut(s)	29
GluI	GCNGC	5 cut(s)	408, 1500, 1814, 2534, 2991
GsuI	CTGGAG	3 cut(s)	279, 1872, 2804
HaeIII	GGCC	8 cut(s)	190, 535, 736, 1281, 1439, 1689, 1793, 1924
HapII	CCGG	2 cut(s)	75, 156
HgaI	GACGC	3 cut(s)	141, 2432, 2900
HhaI	GCGC	1 cut(s)	30
Hin1I	GRCGYC	1 cut(s)	2911
Hin6I	GCGC	1 cut(s)	28
HinP1I	GCGC	1 cut(s)	28
HincII	GTYRAC	1 cut(s)	604
HindII	GTYRAC	1 cut(s)	604
HindIII	AAGCTT	2 cut(s)	182, 2275
HpaII	CCGG	2 cut(s)	75, 156
HphI	GGTGA	8 cut(s)	166, 848, 1484, 2054, 2569, 2666, 2722, 3193
Hpy166II	GTNNAC	4 cut(s)	604, 2045, 2608, 3218
Hpy8I	GTNNAC	4 cut(s)	604, 2045, 2608, 3218
HpyCH4III	ACNGT	5 cut(s)	485, 631, 1257, 2485, 2968
HpyCH4IV	ACGT	3 cut(s)	1419, 1759, 2866
HpyF10VI	GCNNNNNNNGC	8 cut(s)	36, 122, 158, 1921, 2063, 2533, 2539, 3224
HpySE526I	ACGT	3 cut(s)	1419, 1759, 2866
Hsp92I	GRCGYC	1 cut(s)	2911
HspAI	GCGC	1 cut(s)	28
Kpn2I	TCCGGA	1 cut(s)	155
Ksp22I	TGATCA	2 cut(s)	349, 2703
Lsp1109I	GCAGC	5 cut(s)	419, 1511, 1825, 2520, 2977
LweI	GCATC	6 cut(s)	1015, 1744, 2093, 2259, 2687, 3652
MaeI	CTAG	6 cut(s)	519, 642, 1307, 1605, 2186, 3440
MaeII	ACGT	3 cut(s)	1419, 1759, 2866
MaeIII	GTNAC	6 cut(s)	317, 521, 2710, 2725, 3000, 3205
MfeI	CAATTG	3 cut(s)	2619, 2740, 3228
MflI	RGATCY	7 cut(s)	217, 950, 970, 1825, 3070, 3121, 3493
MhlI	GDGCHC	7 cut(s)	163, 490, 1185, 1468, 2861, 3025, 3220
MlsI	TGGCCA	3 cut(s)	1281, 1793, 1924
MluNI	TGGCCA	3 cut(s)	1281, 1793, 1924
MlyI	GAGTC	4 cut(s)	314, 908, 1637, 2817
MmeI	TCCRAC	6 cut(s)	569, 792, 891, 1788, 2049, 2430
Mox20I	TGGCCA	3 cut(s)	1281, 1793, 1924
Mph1103I	ATGCAT	2 cut(s)	669, 2158
MroI	TCCGGA	1 cut(s)	155
MscI	TGGCCA	3 cut(s)	1281, 1793, 1924
MslI	CAYNNNNRTG	3 cut(s)	1589, 1701, 2298
Msp20I	TGGCCA	3 cut(s)	1281, 1793, 1924
MspA1I	CMGCKG	1 cut(s)	1816
MspCI	CTTAAG	1 cut(s)	1343
MspI	CCGG	2 cut(s)	75, 156
MspR9I	CCNGG	9 cut(s)	76, 258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
MunI	CAATTG	3 cut(s)	2619, 2740, 3228
Mva1269I	GAATGC	2 cut(s)	2254, 3337
MvaI	CCWGG	8 cut(s)	258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
MvnI	CGCG	1 cut(s)	30
MwoI	GCNNNNNNNGC	8 cut(s)	36, 122, 158, 1921, 2063, 2533, 2539, 3224
NciI	CCSGG	1 cut(s)	76
NcoI	CCATGG	1 cut(s)	2500
NdeI	CATATG	1 cut(s)	3261
NlaIV	GGNNCC	9 cut(s)	456, 534, 655, 952, 1985, 2012, 2860, 3022, 3307
NmuCI	GTSAC	3 cut(s)	317, 2710, 3000
NsiI	ATGCAT	2 cut(s)	669, 2158
NspI	RCATGY	3 cut(s)	2156, 2630, 3113
NspV	TTCGAA	2 cut(s)	14, 2564
PaeI	GCATGC	1 cut(s)	2156
PagI	TCATGA	2 cut(s)	940, 2296
PceI	AGGCCT	3 cut(s)	190, 1439, 1689
PciI	ACATGT	1 cut(s)	3109
PctI	GAATGC	2 cut(s)	2254, 3337
PfeI	GAWTC	9 cut(s)	673, 794, 872, 1568, 1713, 2174, 2338, 2357, 2566
PfoI	TCCNGGA	1 cut(s)	3196
PkrI	GCNGC	5 cut(s)	409, 1501, 1815, 2535, 2992
PleI	GAGTC	4 cut(s)	314, 908, 1636, 2816
PpsI	GAGTC	4 cut(s)	314, 908, 1636, 2816
Ppu21I	YACGTR	1 cut(s)	1760
PscI	ACATGT	1 cut(s)	3109
PsiI	TTATAA	1 cut(s)	2147
Psp124BI	GAGCTC	1 cut(s)	1185
Psp6I	CCWGG	8 cut(s)	256, 1439, 1481, 1617, 1689, 3168, 3196, 3292
PspGI	CCWGG	8 cut(s)	256, 1439, 1481, 1617, 1689, 3168, 3196, 3292
PspN4I	GGNNCC	9 cut(s)	456, 534, 655, 952, 1985, 2012, 2860, 3022, 3307
PspPI	GGNCC	8 cut(s)	455, 533, 654, 734, 1013, 1202, 3306, 3595
PsrI	GAACNNNNNNTAC	2 cut(s)	2919, 2951
PstI	CTGCAG	4 cut(s)	1501, 1843, 2652, 2995
PstNI	CAGNNNCTG	2 cut(s)	863, 3566
PsuI	RGATCY	7 cut(s)	217, 950, 970, 1825, 3070, 3121, 3493
PvuII	CAGCTG	1 cut(s)	1816
RsaI	GTAC	7 cut(s)	1210, 1425, 1528, 1583, 1870, 1885, 1952
RsaNI	GTAC	7 cut(s)	1209, 1424, 1527, 1582, 1869, 1884, 1951
RseI	CAYNNNNRTG	3 cut(s)	1589, 1701, 2298
SacI	GAGCTC	1 cut(s)	1185
SatI	GCNGC	5 cut(s)	408, 1500, 1814, 2534, 2991
Sau96I	GGNCC	8 cut(s)	455, 533, 654, 734, 1013, 1202, 3306, 3595
SbfI	CCTGCAGG	1 cut(s)	2652
SchI	GAGTC	4 cut(s)	314, 908, 1637, 2817
ScrFI	CCNGG	9 cut(s)	76, 258, 1441, 1483, 1619, 1691, 3170, 3198, 3294
SdaI	CCTGCAGG	1 cut(s)	2652
SduI	GDGCHC	7 cut(s)	163, 490, 1185, 1468, 2861, 3025, 3220
SfaNI	GCATC	6 cut(s)	1015, 1744, 2093, 2259, 2687, 3652
SfcI	CTRYAG	8 cut(s)	1253, 1497, 1839, 2136, 2608, 2648, 2991, 3076
SfuI	TTCGAA	2 cut(s)	14, 2564
SinI	GGWCC	6 cut(s)	455, 654, 1013, 1202, 3306, 3595
SmiMI	CAYNNNNRTG	3 cut(s)	1589, 1701, 2298
SmlI	CTYRAG	2 cut(s)	1343, 3085
SmoI	CTYRAG	2 cut(s)	1343, 3085
SpeI	ACTAGT	1 cut(s)	3439
SphI	GCATGC	1 cut(s)	2156
Sse8387I	CCTGCAGG	1 cut(s)	2652
SseBI	AGGCCT	3 cut(s)	190, 1439, 1689
SsiI	CCGC	6 cut(s)	30, 1339, 1372, 1899, 2095, 2837
SspI	AATATT	2 cut(s)	129, 2194
SspMI	CTAG	6 cut(s)	519, 642, 1307, 1605, 2186, 3440
SstI	GAGCTC	1 cut(s)	1185
StuI	AGGCCT	3 cut(s)	190, 1439, 1689
StyD4I	CCNGG	9 cut(s)	74, 256, 1439, 1481, 1617, 1689, 3168, 3196, 3292
StyI	CCWWGG	4 cut(s)	641, 933, 1604, 2500
TaaI	ACNGT	5 cut(s)	485, 631, 1257, 2485, 2968
TaiI	ACGT	3 cut(s)	1422, 1762, 2869
TaqI	TCGA	9 cut(s)	14, 311, 402, 1120, 1228, 1863, 1974, 2564, 2661
TatI	WGTACW	3 cut(s)	1208, 1581, 1950
TfiI	GAWTC	9 cut(s)	673, 794, 872, 1568, 1713, 2174, 2338, 2357, 2566
TscAI	CASTG	8 cut(s)	46, 118, 444, 490, 514, 571, 2488, 3571
TseFI	GTSAC	3 cut(s)	317, 2710, 3000
TseI	GCWGC	5 cut(s)	407, 1499, 1813, 2533, 2990
Tsp45I	GTSAC	3 cut(s)	317, 2710, 3000
TspGWI	ACGGA	5 cut(s)	983, 2899, 3061, 3521, 3587
TspRI	CASTG	8 cut(s)	46, 118, 444, 490, 514, 571, 2488, 3571
Vha464I	CTTAAG	1 cut(s)	1343
VneI	GTGCAC	1 cut(s)	3216
VpaK11BI	GGWCC	6 cut(s)	455, 654, 1013, 1202, 3306, 3595
XapI	RAATTY	7 cut(s)	358, 416, 622, 893, 2069, 2595, 3639
XceI	RCATGY	3 cut(s)	2156, 2630, 3113
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2446
XmaJI	CCTAGG	2 cut(s)	641, 1604
XmiI	GTMKAC	1 cut(s)	2607
XspI	CTAG	6 cut(s)	519, 642, 1307, 1605, 2186, 3440
Zsp2I	ATGCAT	2 cut(s)	669, 2158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.