FvH4_4g14500

E3 ubiquitin-protein ligase LUL4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014114
Physical Location & Seq
Forward (+)
18168618 .. 18169801
1184 bp
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UTR
Exon/CDS
Intron
FvH4_4g14500.t1

Sequence Viewer

Length: 501 bp
ATGCATGTTTTCTTCAAGTCTAAGGTCATCAAACACTTGAAGTCCCTGAAAACAAAAATGCCCAGATTGTGCATTTTCCCAATTGGGGGATGTTTTGATGGGTGCAGAGACCACACTCAGCAGTCCTCAGGGTTTGGCACAAGGATTTGGAACCTGACTGACAAGCCTGTGGAGCTTCAGATAAGAGTTGGTTCCATATTGAAGAAGGTGCACACTTTGAAACCAGGCTCTTCCAAGAGACTGAAATGCAAGAGCATATACAAAGCTTATATGCCTGGAGAGAATGGCAAGGCAGGTATGAAGAGCTTGCTGTATTACTATGATGAGACTTGTCACCCTTATGTTTGGATTCATGAGACTGGTGGTGATAGCATGAGGATGGTTAAGCAGCAATATATCAGCCTTGAGGATTTGAGGGATTGTTGTGAGATCAGAATCTTTAGGGATCATCATCGAGGCTGCTTATCTGTCAGGAAAAAACCCAGGCCAGACTTTTGCTGA

Protein Analysis

167

Amino Acids

19.29

Weight (kDa)

9.62

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06145
fragaria_vesca FvH4_4g14500
malus_domestica MD13G1183200.v1.1 MD16G1184100.v1.1
prunus_persica Prupe.1G151500_v2.0.a1
pyrus_communis pycom13g15850 pycom16g15460
rosa_chinensis RchiOBHm_Chr4g0418331
rosa_laevigata RLG00000007875
rosa_multiflora Rmu_sc0011505.1_g000002
rosa_roxburghii Rroxscaffold_5G00361520
rosa_rugosa Rorug04G0152200
rosa_samantha Rh4AG213800 Rh4BG216600 Rh4CG225400 Rh4DG212300
rosa_wichuraiana Rw4G018160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 284
AclWI GGATC 1 cut(s) 453
AcuI CTGAAG 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 85, 86
AgsI TTSAA 4 cut(s) 16, 40, 202, 220
AjnI CCWGG 3 cut(s) 223, 274, 482
AluBI AGCT 3 cut(s) 175, 266, 306
AluI AGCT 3 cut(s) 175, 266, 306
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 4 cut(s) 102, 232, 320, 350
Alw44I GTGCAC 1 cut(s) 209
AlwI GGATC 1 cut(s) 453
AoxI GGCC 1 cut(s) 485
ApaLI GTGCAC 1 cut(s) 209
ApeKI GCWGC 2 cut(s) 388, 459
AsuHPI GGTGA 2 cut(s) 326, 377
AxyI CCTNAGG 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 400, 446
BccI CCATC 2 cut(s) 92, 373
BciT130I CCWGG 3 cut(s) 225, 276, 484
BcoDI GTCTC 4 cut(s) 102, 232, 320, 350
BfuAI ACCTGC 1 cut(s) 284
BisI GCNGC 2 cut(s) 389, 460
BlsI GCNGC 2 cut(s) 390, 461
Bme1390I CCNGG 3 cut(s) 225, 276, 484
BmiI GGNNCC 2 cut(s) 152, 193
BmrFI CCNGG 3 cut(s) 225, 276, 484
BpmI CTGGAG 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 425
BsaBI GATNNNNATC 2 cut(s) 434, 450
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 482
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 86
Bse1I ACTGG 1 cut(s) 364
Bse21I CCTNAGG 1 cut(s) 127
Bse8I GATNNNNATC 2 cut(s) 434, 450
BseBI CCWGG 3 cut(s) 225, 276, 484
BseDI CCNNGG 1 cut(s) 482
BseGI GGATG 2 cut(s) 95, 384
BseJI GATNNNNATC 2 cut(s) 434, 450
BseLI CCNNNNNNNGG 2 cut(s) 85, 86
BseMII CTCAG 2 cut(s) 131, 141
BseNI ACTGG 1 cut(s) 364
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 2 cut(s) 400, 446
BsgI GTGCAG 1 cut(s) 124
BshFI GGCC 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 213
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 2 cut(s) 85, 86
BsmAI GTCTC 4 cut(s) 102, 232, 320, 350
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 1 cut(s) 487
Bso31I GGTCTC 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 2 cut(s) 429, 445
BspANI GGCC 1 cut(s) 487
BspCNI CTCAG 2 cut(s) 130, 140
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 152, 193
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 1 cut(s) 453
BspQI GCTCTTC 2 cut(s) 235, 296
BspTNI GGTCTC 1 cut(s) 102
BsrI ACTGG 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 482
BssMI GATC 2 cut(s) 429, 445
Bst2UI CCWGG 3 cut(s) 225, 276, 484
Bst6I CTCTTC 2 cut(s) 235, 296
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 3 cut(s) 21, 117, 127
BstF5I GGATG 2 cut(s) 95, 384
BstKTI GATC 2 cut(s) 432, 448
BstMAI GTCTC 4 cut(s) 102, 232, 320, 350
BstMBI GATC 2 cut(s) 429, 445
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 3 cut(s) 225, 276, 484
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 3 cut(s) 223, 274, 482
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 400, 446
Bsu36I CCTNAGG 1 cut(s) 127
BsuRI GGCC 1 cut(s) 487
BtsCI GGATG 2 cut(s) 95, 384
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 308
CciI TCATGA 1 cut(s) 352
CviAII CATG 3 cut(s) 5, 353, 373
CviJI RGCY 8 cut(s) 166, 175, 228, 266, 306, 402, 459, 487
CviKI_1 RGCY 8 cut(s) 166, 175, 228, 266, 306, 402, 459, 487
DdeI CTNAG 3 cut(s) 21, 117, 127
DpnI GATC 2 cut(s) 431, 447
DpnII GATC 2 cut(s) 429, 445
Eam1104I CTCTTC 2 cut(s) 235, 296
EarI CTCTTC 2 cut(s) 235, 296
Eco31I GGTCTC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 161
Eco81I CCTNAGG 1 cut(s) 127
EcoRII CCWGG 3 cut(s) 223, 274, 482
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 3 cut(s) 8, 356, 376
FaqI GGGAC 1 cut(s) 28
FatI CATG 3 cut(s) 4, 352, 372
Fnu4HI GCNGC 2 cut(s) 389, 460
FokI GGATG 2 cut(s) 102, 391
Fsp4HI GCNGC 2 cut(s) 389, 460
GluI GCNGC 2 cut(s) 389, 460
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 1 cut(s) 487
Hin1II CATG 3 cut(s) 8, 356, 376
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 2 cut(s) 349, 435
HphI GGTGA 2 cut(s) 326, 377
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 2 cut(s) 180, 434
Hpy188III TCNNGA 2 cut(s) 353, 472
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 1 cut(s) 199
HpyCH4V TGCA 5 cut(s) 4, 72, 105, 211, 249
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 3 cut(s) 21, 117, 127
Hsp92II CATG 3 cut(s) 8, 356, 376
Kzo9I GATC 2 cut(s) 429, 445
LguI GCTCTTC 2 cut(s) 235, 296
LmnI GCTCC 1 cut(s) 172
Lsp1109I GCAGC 2 cut(s) 400, 446
MaeIII GTNAC 1 cut(s) 332
MalI GATC 2 cut(s) 431, 447
MboI GATC 2 cut(s) 429, 445
MboII GAAGA 4 cut(s) 4, 214, 222, 313
MfeI CAATTG 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 1 cut(s) 81
MnlI CCTC 5 cut(s) 136, 369, 400, 408, 449
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 2 cut(s) 339, 377
MspR9I CCNGG 3 cut(s) 225, 276, 484
MunI CAATTG 1 cut(s) 81
MvaI CCWGG 3 cut(s) 225, 276, 484
MwoI GCNNNNNNNGC 1 cut(s) 172
NdeII GATC 2 cut(s) 429, 445
NlaIII CATG 3 cut(s) 8, 356, 376
NlaIV GGNNCC 2 cut(s) 152, 193
NmuCI GTSAC 1 cut(s) 332
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PagI TCATGA 1 cut(s) 352
PciSI GCTCTTC 2 cut(s) 235, 296
PfeI GAWTC 2 cut(s) 349, 435
PkrI GCNGC 2 cut(s) 390, 461
Psp6I CCWGG 3 cut(s) 223, 274, 482
PspGI CCWGG 3 cut(s) 223, 274, 482
PspN4I GGNNCC 2 cut(s) 152, 193
RseI CAYNNNNRTG 2 cut(s) 339, 377
SapI GCTCTTC 2 cut(s) 235, 296
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 389, 460
Sau3AI GATC 2 cut(s) 429, 445
ScrFI CCNGG 3 cut(s) 225, 276, 484
SduI GDGCHC 1 cut(s) 213
SetI ASST 7 cut(s) 27, 156, 177, 210, 268, 298, 308
SmiMI CAYNNNNRTG 2 cut(s) 339, 377
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 1 cut(s) 81
StyD4I CCNGG 3 cut(s) 223, 274, 482
TaqI TCGA 1 cut(s) 454
TasI AATT 1 cut(s) 81
TfiI GAWTC 2 cut(s) 349, 435
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 388, 459
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 2 cut(s) 314, 341
VneI GTGCAC 1 cut(s) 209
XceI RCATGY 1 cut(s) 8
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.