pycom16g15460

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014114
Physical Location & Seq
Forward (+)
11404228 .. 11404644
417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g15460.1

Sequence Viewer

Length: 417 bp
ATGGATGCAGGGATCGACACACAGCAATCCTCAGGATTTGGAACTAGGGTTTGGAACCTGAGTGACAAGCCTGTGGAGCTGCAGATAAGAGTTGGGTCCATACTAAAAAAAGTTCACACTTTGAAACCAGGCTGCTCAAAGAGGCTGAAATGCAAGAGCATATACAAGGCCTACATGCCCGGCGGAGAGAGTGGCGGCAGCGGCGGTGGTGGCATGAAGGGCTTGCTGTATTACTACGACGAGACCTGCCACCCTTACGTTTGGATTCATGACACTGGTGGTGATACTTTGAGGATGGTCAAGCAGCAATATATCAGCCTTGAGGACCTCAGGGACTCTTGTGAGATCATGATTTTCAGGGATCATCAGAGAGGCTGCGTTTTGGTTCGGAAGAAACCGAGGCCGGATTTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.54

Weight (kDa)

9.04

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06145
fragaria_vesca FvH4_4g14500
malus_domestica MD13G1183200.v1.1 MD16G1184100.v1.1
prunus_persica Prupe.1G151500_v2.0.a1
pyrus_communis pycom13g15850 pycom16g15460
rosa_chinensis RchiOBHm_Chr4g0418331
rosa_laevigata RLG00000007875
rosa_multiflora Rmu_sc0011505.1_g000002
rosa_roxburghii Rroxscaffold_5G00361520
rosa_rugosa Rorug04G0152200
rosa_samantha Rh4AG213800 Rh4BG216600 Rh4CG225400 Rh4DG212300
rosa_wichuraiana Rw4G018160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 254
AciI CCGC 4 cut(s) 183, 195, 201, 204
AclWI GGATC 2 cut(s) 20, 369
AgsI TTSAA 1 cut(s) 124
AjnI CCWGG 1 cut(s) 127
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 1 cut(s) 79
AluI AGCT 1 cut(s) 79
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 2 cut(s) 20, 369
AoxI GGCC 2 cut(s) 168, 401
ApeKI GCWGC 5 cut(s) 79, 132, 198, 304, 375
AspS9I GGNCC 2 cut(s) 96, 325
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 1 cut(s) 293
AvaII GGWCC 2 cut(s) 96, 325
AxyI CCTNAGG 2 cut(s) 31, 329
BbvI GCAGC 5 cut(s) 66, 119, 210, 316, 362
BccI CCATC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 129
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 236
BfaI CTAG 1 cut(s) 45
BfmI CTRYAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 254
BisI GCNGC 7 cut(s) 80, 133, 196, 199, 202, 305, 376
BlsI GCNGC 7 cut(s) 81, 134, 197, 200, 203, 306, 377
Bme1390I CCNGG 2 cut(s) 129, 180
Bme18I GGWCC 2 cut(s) 96, 325
BmgT120I GGNCC 2 cut(s) 96, 325
BmiI GGNNCC 2 cut(s) 56, 97
BmrFI CCNGG 2 cut(s) 129, 180
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 280
Bse21I CCTNAGG 2 cut(s) 31, 329
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 2 cut(s) 10, 300
BseMII CTCAG 3 cut(s) 45, 50, 343
BseNI ACTGG 1 cut(s) 280
BseXI GCAGC 5 cut(s) 66, 119, 210, 316, 362
BshFI GGCC 2 cut(s) 170, 403
BsiSI CCGG 2 cut(s) 180, 404
BslFI GGGAC 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 236
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 2 cut(s) 170, 403
Bso31I GGTCTC 1 cut(s) 236
Bsp143I GATC 3 cut(s) 12, 345, 361
BspACI CCGC 4 cut(s) 183, 195, 201, 204
BspANI GGCC 2 cut(s) 170, 403
BspCNI CTCAG 3 cut(s) 44, 51, 342
BspHI TCATGA 2 cut(s) 268, 348
BspLI GGNNCC 2 cut(s) 56, 97
BspMAI CTGCAG 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 2 cut(s) 20, 369
BspTNI GGTCTC 1 cut(s) 236
BsrI ACTGG 1 cut(s) 280
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 3 cut(s) 12, 345, 361
Bst2UI CCWGG 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 3 cut(s) 31, 59, 329
BstF5I GGATG 2 cut(s) 10, 300
BstKTI GATC 3 cut(s) 15, 348, 364
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 3 cut(s) 12, 345, 361
BstMWI GCNNNNNNNGC 4 cut(s) 76, 201, 210, 219
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 178
BstSCI CCNGG 2 cut(s) 127, 178
BstSFI CTRYAG 1 cut(s) 80
BstV1I GCAGC 5 cut(s) 66, 119, 210, 316, 362
Bsu36I CCTNAGG 2 cut(s) 31, 329
BsuRI GGCC 2 cut(s) 170, 403
BtsCI GGATG 2 cut(s) 10, 300
BtsIMutI CAGTG 1 cut(s) 273
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 1 cut(s) 224
CciI TCATGA 2 cut(s) 268, 348
Cfr13I GGNCC 2 cut(s) 96, 325
CviAII CATG 4 cut(s) 175, 214, 269, 349
CviJI RGCY 9 cut(s) 70, 79, 132, 145, 170, 222, 318, 375, 403
CviKI_1 RGCY 9 cut(s) 70, 79, 132, 145, 170, 222, 318, 375, 403
DdeI CTNAG 3 cut(s) 31, 59, 329
DpnI GATC 3 cut(s) 14, 347, 363
DpnII GATC 3 cut(s) 12, 345, 361
EciI GGCGGA 1 cut(s) 198
Eco147I AGGCCT 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 96, 325
Eco81I CCTNAGG 2 cut(s) 31, 329
EcoO109I RGGNCCY 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 127
FaeI CATG 4 cut(s) 178, 217, 272, 352
FaiI YATR 8 cut(s) 101, 161, 163, 176, 215, 270, 312, 350
FaqI GGGAC 1 cut(s) 347
FatI CATG 4 cut(s) 174, 213, 268, 348
Fnu4HI GCNGC 7 cut(s) 80, 133, 196, 199, 202, 305, 376
FokI GGATG 2 cut(s) 17, 307
Fsp4HI GCNGC 7 cut(s) 80, 133, 196, 199, 202, 305, 376
FspBI CTAG 1 cut(s) 45
GluI GCNGC 7 cut(s) 80, 133, 196, 199, 202, 305, 376
HaeIII GGCC 2 cut(s) 170, 403
HapII CCGG 2 cut(s) 180, 404
Hin1II CATG 4 cut(s) 178, 217, 272, 352
HinfI GANTC 2 cut(s) 265, 335
HpaII CCGG 2 cut(s) 180, 404
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 2 cut(s) 369, 390
Hpy188III TCNNGA 3 cut(s) 33, 269, 349
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 3 cut(s) 8, 82, 153
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 201, 210, 219
HpyF3I CTNAG 3 cut(s) 31, 59, 329
HpySE526I ACGT 1 cut(s) 258
Hsp92II CATG 4 cut(s) 178, 217, 272, 352
Kzo9I GATC 3 cut(s) 12, 345, 361
LmnI GCTCC 1 cut(s) 76
Lsp1109I GCAGC 5 cut(s) 66, 119, 210, 316, 362
MaeI CTAG 1 cut(s) 45
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 62
MalI GATC 3 cut(s) 14, 347, 363
MboI GATC 3 cut(s) 12, 345, 361
MboII GAAGA 1 cut(s) 403
MlyI GAGTC 1 cut(s) 329
MnlI CCTC 7 cut(s) 40, 135, 285, 316, 338, 365, 393
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 2 cut(s) 180, 404
MspR9I CCNGG 2 cut(s) 129, 180
MvaI CCWGG 1 cut(s) 129
MwoI GCNNNNNNNGC 4 cut(s) 76, 201, 210, 219
NciI CCSGG 1 cut(s) 180
NdeII GATC 3 cut(s) 12, 345, 361
NlaIII CATG 4 cut(s) 178, 217, 272, 352
NlaIV GGNNCC 2 cut(s) 56, 97
NmuCI GTSAC 1 cut(s) 62
NspI RCATGY 1 cut(s) 178
PagI TCATGA 2 cut(s) 268, 348
PceI AGGCCT 1 cut(s) 170
PfeI GAWTC 1 cut(s) 265
PkrI GCNGC 7 cut(s) 81, 134, 197, 200, 203, 306, 377
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
PpuMI RGGWCCY 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 2 cut(s) 56, 97
PspPI GGNCC 2 cut(s) 96, 325
PspPPI RGGWCCY 1 cut(s) 325
PstI CTGCAG 1 cut(s) 84
SatI GCNGC 7 cut(s) 80, 133, 196, 199, 202, 305, 376
Sau3AI GATC 3 cut(s) 12, 345, 361
Sau96I GGNCC 2 cut(s) 96, 325
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 129, 180
SetI ASST 5 cut(s) 60, 81, 248, 261, 330
SfcI CTRYAG 1 cut(s) 80
SinI GGWCC 2 cut(s) 96, 325
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 4 cut(s) 183, 195, 201, 204
SspMI CTAG 1 cut(s) 45
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 127, 178
TaiI ACGT 1 cut(s) 261
TaqI TCGA 1 cut(s) 15
TauI GCSGC 2 cut(s) 198, 204
TfiI GAWTC 1 cut(s) 265
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 5 cut(s) 79, 132, 198, 304, 375
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 2 cut(s) 230, 257
TspRI CASTG 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 96, 325
XceI RCATGY 1 cut(s) 178
XspI CTAG 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.