MD16G1184100.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014114
Physical Location & Seq
Forward (+)
15816284 .. 15816892
609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1184100.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGGGGTCATTACACAGGTTATATATGGACTTAAAGATGTCAAATATGAGTGGTGTGAATCTTCTGCAACACAAAAAACCGAAAAAATGCATGTTGTCTTTAAGTCCAAGGTGACCAAAGTCACCAAACATTTCAAGTCACTCCAATCAAAAATGCCCAGATTTAGTATCTTCCCAACTGGTGGCTGCTTTGATGGATGCAGGGATCACACACAGCAATCCTCAGGATTTGGAACTAGGGTTTGGAACCTGAGTGACAAGCCTGTGGAGCTGCAGATTAGAGTTGGGTCCATACTAAAAAAAGTTCACACTTTGAAACCAGGCTGCTCAAAGAGGCTAAAATGCAAGAGCATATACAAGGCCTACATGCCCGGCGGAGAGAGTGGCGGCAGCGGCGGTGGTGGCATGAAGGGCTTGCTGTATTACTACGACGAGACCTGCCACCCTTACGTTTGGATTCATGACACTGGTGGTGATACTTTGAGGATGGTCAAGCAGCAATATATCAGCCTTGAGGACCTCAGGGACTGTTGTGAGATCAGAATTTTCAGGGATCATCAGAGAGGCTGCGTTTTGGTTCGGAAGAAACCGAGGCCGGAATTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

23.0

Weight (kDa)

9.44

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06145
fragaria_vesca FvH4_4g14500
malus_domestica MD13G1183200.v1.1 MD16G1184100.v1.1
prunus_persica Prupe.1G151500_v2.0.a1
pyrus_communis pycom13g15850 pycom16g15460
rosa_chinensis RchiOBHm_Chr4g0418331
rosa_laevigata RLG00000007875
rosa_multiflora Rmu_sc0011505.1_g000002
rosa_roxburghii Rroxscaffold_5G00361520
rosa_rugosa Rorug04G0152200
rosa_samantha Rh4AG213800 Rh4BG216600 Rh4CG225400 Rh4DG212300
rosa_wichuraiana Rw4G018160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 446
AccB7I CCANNNNNTGG 1 cut(s) 182
AciI CCGC 4 cut(s) 375, 387, 393, 396
AclWI GGATC 2 cut(s) 213, 561
AcsI RAATTY 2 cut(s) 543, 599
AfiI CCNNNNNNNGG 1 cut(s) 182
AgsI TTSAA 2 cut(s) 136, 316
AjnI CCWGG 1 cut(s) 319
AjuI GAANNNNNNNTTGG 2 cut(s) 226, 258
AluBI AGCT 1 cut(s) 271
AluI AGCT 1 cut(s) 271
Alw26I GTCTC 1 cut(s) 428
AlwI GGATC 2 cut(s) 213, 561
AlwNI CAGNNNCTG 1 cut(s) 528
AoxI GGCC 2 cut(s) 360, 593
ApeKI GCWGC 6 cut(s) 186, 271, 324, 390, 496, 567
ApoI RAATTY 2 cut(s) 543, 599
AspS9I GGNCC 2 cut(s) 288, 517
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 3 cut(s) 115, 124, 485
AvaII GGWCC 2 cut(s) 288, 517
AxyI CCTNAGG 2 cut(s) 223, 521
BbvI GCAGC 6 cut(s) 173, 258, 311, 402, 508, 554
BccI CCATC 2 cut(s) 188, 481
BciT130I CCWGG 1 cut(s) 321
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 428
BfaI CTAG 1 cut(s) 237
BfmI CTRYAG 1 cut(s) 272
BfuAI ACCTGC 1 cut(s) 446
BisI GCNGC 8 cut(s) 187, 272, 325, 388, 391, 394, 497, 568
BlsI GCNGC 8 cut(s) 188, 273, 326, 389, 392, 395, 498, 569
Bme1390I CCNGG 2 cut(s) 321, 372
Bme18I GGWCC 2 cut(s) 288, 517
BmgT120I GGNCC 2 cut(s) 288, 517
BmiI GGNNCC 2 cut(s) 248, 289
BmrFI CCNGG 2 cut(s) 321, 372
BmsI GCATC 1 cut(s) 188
BoxI GACNNNNGTC 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 533
BpuMI CCSGG 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 428
BsaJI CCNNGG 2 cut(s) 108, 590
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 184, 472
Bse21I CCTNAGG 2 cut(s) 223, 521
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 108, 590
BseGI GGATG 2 cut(s) 203, 492
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMII CTCAG 3 cut(s) 237, 242, 535
BseNI ACTGG 2 cut(s) 184, 472
BseXI GCAGC 6 cut(s) 173, 258, 311, 402, 508, 554
BshFI GGCC 2 cut(s) 362, 595
BsiSI CCGG 2 cut(s) 372, 596
BslFI GGGAC 1 cut(s) 539
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 539
BsnI GGCC 2 cut(s) 362, 595
Bso31I GGTCTC 1 cut(s) 428
Bsp143I GATC 3 cut(s) 205, 537, 553
BspACI CCGC 4 cut(s) 375, 387, 393, 396
BspANI GGCC 2 cut(s) 362, 595
BspCNI CTCAG 3 cut(s) 236, 243, 534
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 2 cut(s) 248, 289
BspMAI CTGCAG 1 cut(s) 276
BspMI ACCTGC 1 cut(s) 446
BspPI GGATC 2 cut(s) 213, 561
BspTNI GGTCTC 1 cut(s) 428
BsrI ACTGG 2 cut(s) 184, 472
BssECI CCNNGG 2 cut(s) 108, 590
BssMI GATC 3 cut(s) 205, 537, 553
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 416
BstDEI CTNAG 3 cut(s) 223, 251, 521
BstEII GGTNACC 1 cut(s) 112
BstF5I GGATG 2 cut(s) 203, 492
BstKTI GATC 3 cut(s) 208, 540, 556
BstMAI GTCTC 1 cut(s) 428
BstMBI GATC 3 cut(s) 205, 537, 553
BstMWI GCNNNNNNNGC 4 cut(s) 268, 393, 402, 411
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 2 cut(s) 95, 370
BstPAI GACNNNNGTC 1 cut(s) 119
BstPI GGTNACC 1 cut(s) 112
BstSCI CCNGG 2 cut(s) 319, 370
BstSFI CTRYAG 1 cut(s) 272
BstV1I GCAGC 6 cut(s) 173, 258, 311, 402, 508, 554
Bsu36I CCTNAGG 2 cut(s) 223, 521
BsuRI GGCC 2 cut(s) 362, 595
BtsCI GGATG 2 cut(s) 203, 492
BtsIMutI CAGTG 1 cut(s) 465
BveI ACCTGC 1 cut(s) 446
Cac8I GCNNGC 1 cut(s) 416
CaiI CAGNNNCTG 1 cut(s) 528
CciI TCATGA 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 288, 517
CviAII CATG 4 cut(s) 92, 367, 406, 461
DdeI CTNAG 3 cut(s) 223, 251, 521
DpnI GATC 3 cut(s) 207, 539, 555
DpnII GATC 3 cut(s) 205, 537, 553
EciI GGCGGA 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 428
Eco47I GGWCC 2 cut(s) 288, 517
Eco81I CCTNAGG 2 cut(s) 223, 521
Eco91I GGTNACC 1 cut(s) 112
EcoO109I RGGNCCY 1 cut(s) 517
EcoO65I GGTNACC 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 4 cut(s) 95, 370, 409, 464
FaqI GGGAC 1 cut(s) 539
FatI CATG 4 cut(s) 91, 366, 405, 460
Fnu4HI GCNGC 8 cut(s) 187, 272, 325, 388, 391, 394, 497, 568
FokI GGATG 2 cut(s) 210, 499
Fsp4HI GCNGC 8 cut(s) 187, 272, 325, 388, 391, 394, 497, 568
FspBI CTAG 1 cut(s) 237
GluI GCNGC 8 cut(s) 187, 272, 325, 388, 391, 394, 497, 568
HaeIII GGCC 2 cut(s) 362, 595
HapII CCGG 2 cut(s) 372, 596
Hin1II CATG 4 cut(s) 95, 370, 409, 464
HinfI GANTC 2 cut(s) 59, 457
HpaII CCGG 2 cut(s) 372, 596
HphI GGTGA 3 cut(s) 115, 124, 485
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 3 cut(s) 542, 561, 582
Hpy188III TCNNGA 2 cut(s) 225, 461
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 5 cut(s) 68, 91, 201, 274, 345
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 393, 402, 411
HpyF3I CTNAG 3 cut(s) 223, 251, 521
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 4 cut(s) 95, 370, 409, 464
Kzo9I GATC 3 cut(s) 205, 537, 553
LmnI GCTCC 1 cut(s) 268
Lsp1109I GCAGC 6 cut(s) 173, 258, 311, 402, 508, 554
LweI GCATC 1 cut(s) 188
MaeI CTAG 1 cut(s) 237
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 4 cut(s) 112, 121, 138, 254
MalI GATC 3 cut(s) 207, 539, 555
MboI GATC 3 cut(s) 205, 537, 553
MboII GAAGA 3 cut(s) 54, 163, 595
MluCI AATT 2 cut(s) 543, 599
MnlI CCTC 7 cut(s) 232, 327, 477, 508, 530, 557, 585
Mph1103I ATGCAT 1 cut(s) 93
MseI TTAA 2 cut(s) 33, 102
MspA1I CMGCKG 1 cut(s) 393
MspI CCGG 2 cut(s) 372, 596
MspR9I CCNGG 2 cut(s) 321, 372
MvaI CCWGG 1 cut(s) 321
MwoI GCNNNNNNNGC 4 cut(s) 268, 393, 402, 411
NciI CCSGG 1 cut(s) 372
NdeII GATC 3 cut(s) 205, 537, 553
NlaIII CATG 4 cut(s) 95, 370, 409, 464
NlaIV GGNNCC 2 cut(s) 248, 289
NmuCI GTSAC 4 cut(s) 112, 121, 138, 254
NsiI ATGCAT 1 cut(s) 93
NspI RCATGY 2 cut(s) 95, 370
PagI TCATGA 1 cut(s) 460
PceI AGGCCT 1 cut(s) 362
PfeI GAWTC 2 cut(s) 59, 457
PflMI CCANNNNNTGG 1 cut(s) 182
PkrI GCNGC 8 cut(s) 188, 273, 326, 389, 392, 395, 498, 569
PpuMI RGGWCCY 1 cut(s) 517
PshAI GACNNNNGTC 1 cut(s) 119
Psp5II RGGWCCY 1 cut(s) 517
Psp6I CCWGG 1 cut(s) 319
PspEI GGTNACC 1 cut(s) 112
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 2 cut(s) 248, 289
PspPI GGNCC 2 cut(s) 288, 517
PspPPI RGGWCCY 1 cut(s) 517
PstI CTGCAG 1 cut(s) 276
PstNI CAGNNNCTG 1 cut(s) 528
SaqAI TTAA 2 cut(s) 33, 102
SatI GCNGC 8 cut(s) 187, 272, 325, 388, 391, 394, 497, 568
Sau3AI GATC 3 cut(s) 205, 537, 553
Sau96I GGNCC 2 cut(s) 288, 517
ScrFI CCNGG 2 cut(s) 321, 372
SetI ASST 7 cut(s) 21, 114, 252, 273, 440, 453, 522
SfaNI GCATC 1 cut(s) 188
SfcI CTRYAG 1 cut(s) 272
SinI GGWCC 2 cut(s) 288, 517
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 2 cut(s) 543, 599
SseBI AGGCCT 1 cut(s) 362
SsiI CCGC 4 cut(s) 375, 387, 393, 396
SspMI CTAG 1 cut(s) 237
StuI AGGCCT 1 cut(s) 362
StyD4I CCNGG 2 cut(s) 319, 370
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 1 cut(s) 453
TasI AATT 2 cut(s) 543, 599
TauI GCSGC 2 cut(s) 390, 396
TfiI GAWTC 2 cut(s) 59, 457
Tru1I TTAA 2 cut(s) 33, 102
Tru9I TTAA 2 cut(s) 33, 102
TscAI CASTG 1 cut(s) 472
TseFI GTSAC 4 cut(s) 112, 121, 138, 254
TseI GCWGC 6 cut(s) 186, 271, 324, 390, 496, 567
Tsp45I GTSAC 4 cut(s) 112, 121, 138, 254
TspDTI ATGAA 2 cut(s) 422, 449
TspRI CASTG 1 cut(s) 472
Van91I CCANNNNNTGG 1 cut(s) 182
VpaK11BI GGWCC 2 cut(s) 288, 517
XapI RAATTY 2 cut(s) 543, 599
XceI RCATGY 2 cut(s) 95, 370
XspI CTAG 1 cut(s) 237
Zsp2I ATGCAT 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.