MD13G1183200.v1.1

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014114
Physical Location & Seq
Forward (+)
15423586 .. 15424107
522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1183200.v1.1.491

Sequence Viewer

Length: 522 bp
ATGCACGTTGTCCTTAAATCCAAGGTGACCAAAGTCACCAAACATTTCAAGTCCCTGAAATCAAAGATGCCCAGATTTGGTATCTTCCCAACTGGTGGCTGCTTTGATGGATGCAGGGATCACACACAGCAATCCTCAGGATTTGGAACTAGGGTTTGGAACCTGAGTGACAAGCCTGTGGAACTGCAGATAAGAGTTGGGTCCATACTAAAAAAGGTTCACACTTTGAAACCAGGCTCCTCCAAGAGGCTGAAGTGCAAGAGCATATACAAGGCCTATATGCCCGGCGGAGAGGGTGGCGGCAGCGGCCGTGGCAGCATGAGGGGCTTGCTGTATTACTACGACGAAACTTGCCACCCTTACGTTTGGATTCATGACACTGGTGGTGATACATTGAGGATGGTCAAGCAGCAGTATATCAGTCTTGAGGATCTCAGGGAGTGTTGTGAGATCAGGATTTTCAGGGATCATCAGAGAGGCTGCGTTTCGGTTCGGAAGAAACCGAGGCCGGATTTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.7

Weight (kDa)

9.76

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06145
fragaria_vesca FvH4_4g14500
malus_domestica MD13G1183200.v1.1 MD16G1184100.v1.1
prunus_persica Prupe.1G151500_v2.0.a1
pyrus_communis pycom13g15850 pycom16g15460
rosa_chinensis RchiOBHm_Chr4g0418331
rosa_laevigata RLG00000007875
rosa_multiflora Rmu_sc0011505.1_g000002
rosa_roxburghii Rroxscaffold_5G00361520
rosa_rugosa Rorug04G0152200
rosa_samantha Rh4AG213800 Rh4BG216600 Rh4CG225400 Rh4DG212300
rosa_wichuraiana Rw4G018160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AciI CCGC 3 cut(s) 288, 300, 306
AclWI GGATC 3 cut(s) 126, 438, 474
AcoI YGGCCR 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 272
AfiI CCNNNNNNNGG 3 cut(s) 77, 95, 246
AgsI TTSAA 2 cut(s) 49, 229
AjnI CCWGG 1 cut(s) 232
AjuI GAANNNNNNNTTGG 2 cut(s) 139, 171
AlwI GGATC 3 cut(s) 126, 438, 474
AoxI GGCC 3 cut(s) 273, 307, 506
ApeKI GCWGC 5 cut(s) 99, 303, 315, 409, 480
AspS9I GGNCC 1 cut(s) 201
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 3 cut(s) 28, 37, 398
AvaII GGWCC 1 cut(s) 201
AxyI CCTNAGG 1 cut(s) 136
BbvI GCAGC 5 cut(s) 86, 315, 327, 421, 467
BccI CCATC 2 cut(s) 101, 394
BceAI ACGGC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 234
BcnI CCSGG 1 cut(s) 285
BfaI CTAG 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 185
BisI GCNGC 7 cut(s) 100, 301, 304, 307, 316, 410, 481
BlsI GCNGC 7 cut(s) 101, 302, 305, 308, 317, 411, 482
Bme1390I CCNGG 2 cut(s) 234, 285
Bme18I GGWCC 1 cut(s) 201
BmgT120I GGNCC 1 cut(s) 201
BmiI GGNNCC 3 cut(s) 161, 202, 238
BmrFI CCNGG 2 cut(s) 234, 285
BmsI GCATC 2 cut(s) 57, 101
BoxI GACNNNNGTC 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 446
BpuMI CCSGG 1 cut(s) 285
BsaJI CCNNGG 3 cut(s) 21, 310, 503
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 95, 246
Bse1I ACTGG 2 cut(s) 97, 385
Bse21I CCTNAGG 1 cut(s) 136
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 21, 310, 503
BseGI GGATG 2 cut(s) 116, 405
BseLI CCNNNNNNNGG 3 cut(s) 77, 95, 246
BseMII CTCAG 3 cut(s) 150, 155, 448
BseNI ACTGG 2 cut(s) 97, 385
BseRI GAGGAG 1 cut(s) 229
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 5 cut(s) 86, 315, 327, 421, 467
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 3 cut(s) 275, 309, 508
BsiEI CGRYCG 1 cut(s) 310
BsiSI CCGG 2 cut(s) 285, 509
BslFI GGGAC 1 cut(s) 37
BslI CCNNNNNNNGG 3 cut(s) 77, 95, 246
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 3 cut(s) 275, 309, 508
Bsp143I GATC 4 cut(s) 118, 430, 450, 466
BspACI CCGC 3 cut(s) 288, 300, 306
BspANI GGCC 3 cut(s) 275, 309, 508
BspCNI CTCAG 3 cut(s) 149, 156, 447
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 3 cut(s) 161, 202, 238
BspMAI CTGCAG 1 cut(s) 189
BspPI GGATC 3 cut(s) 126, 438, 474
BsrI ACTGG 2 cut(s) 97, 385
BssECI CCNNGG 3 cut(s) 21, 310, 503
BssMI GATC 4 cut(s) 118, 430, 450, 466
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 3 cut(s) 136, 164, 434
BstDSI CCRYGG 1 cut(s) 310
BstEII GGTNACC 1 cut(s) 25
BstENI CCTNNNNNAGG 1 cut(s) 244
BstF5I GGATG 2 cut(s) 116, 405
BstKTI GATC 4 cut(s) 121, 433, 453, 469
BstMBI GATC 4 cut(s) 118, 430, 450, 466
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 4 cut(s) 306, 312, 315, 324
BstNI CCWGG 1 cut(s) 234
BstPAI GACNNNNGTC 1 cut(s) 32
BstPI GGTNACC 1 cut(s) 25
BstSCI CCNGG 2 cut(s) 232, 283
BstSFI CTRYAG 1 cut(s) 185
BstV1I GCAGC 5 cut(s) 86, 315, 327, 421, 467
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BstZI CGGCCG 1 cut(s) 307
Bsu36I CCTNAGG 1 cut(s) 136
BsuRI GGCC 3 cut(s) 275, 309, 508
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 2 cut(s) 116, 405
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 329
CciI TCATGA 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 201
CviAII CATG 2 cut(s) 319, 374
CviJI RGCY 9 cut(s) 99, 175, 237, 250, 275, 309, 327, 480, 508
CviKI_1 RGCY 9 cut(s) 99, 175, 237, 250, 275, 309, 327, 480, 508
DdeI CTNAG 3 cut(s) 136, 164, 434
DpnI GATC 4 cut(s) 120, 432, 452, 468
DpnII GATC 4 cut(s) 118, 430, 450, 466
EaeI YGGCCR 1 cut(s) 307
EagI CGGCCG 1 cut(s) 307
EciI GGCGGA 1 cut(s) 303
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 21
Eco147I AGGCCT 1 cut(s) 275
Eco47I GGWCC 1 cut(s) 201
Eco52I CGGCCG 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 272
Eco81I CCTNAGG 1 cut(s) 136
Eco91I GGTNACC 1 cut(s) 25
EcoNI CCTNNNNNAGG 1 cut(s) 244
EcoO65I GGTNACC 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 2 cut(s) 322, 377
FaiI YATR 8 cut(s) 206, 266, 268, 279, 281, 320, 375, 417
FaqI GGGAC 1 cut(s) 37
FatI CATG 2 cut(s) 318, 373
Fnu4HI GCNGC 7 cut(s) 100, 301, 304, 307, 316, 410, 481
FokI GGATG 2 cut(s) 123, 412
Fsp4HI GCNGC 7 cut(s) 100, 301, 304, 307, 316, 410, 481
FspBI CTAG 1 cut(s) 150
GluI GCNGC 7 cut(s) 100, 301, 304, 307, 316, 410, 481
HaeIII GGCC 3 cut(s) 275, 309, 508
HapII CCGG 2 cut(s) 285, 509
Hin1II CATG 2 cut(s) 322, 377
HinfI GANTC 1 cut(s) 370
HpaII CCGG 2 cut(s) 285, 509
HphI GGTGA 3 cut(s) 28, 37, 398
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 2 cut(s) 474, 495
Hpy188III TCNNGA 4 cut(s) 138, 374, 425, 454
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 347
HpyCH4IV ACGT 2 cut(s) 6, 363
HpyCH4V TGCA 4 cut(s) 4, 114, 187, 258
HpyF10VI GCNNNNNNNGC 4 cut(s) 306, 312, 315, 324
HpyF3I CTNAG 3 cut(s) 136, 164, 434
HpySE526I ACGT 2 cut(s) 6, 363
Hsp92II CATG 2 cut(s) 322, 377
Kzo9I GATC 4 cut(s) 118, 430, 450, 466
LmnI GCTCC 1 cut(s) 242
Lsp1109I GCAGC 5 cut(s) 86, 315, 327, 421, 467
LweI GCATC 2 cut(s) 57, 101
MaeI CTAG 1 cut(s) 150
MaeII ACGT 2 cut(s) 6, 363
MaeIII GTNAC 3 cut(s) 25, 34, 167
MalI GATC 4 cut(s) 120, 432, 452, 468
MboI GATC 4 cut(s) 118, 430, 450, 466
MboII GAAGA 2 cut(s) 76, 508
MflI RGATCY 1 cut(s) 430
MnlI CCTC 9 cut(s) 145, 240, 250, 286, 315, 390, 421, 470, 498
MseI TTAA 1 cut(s) 15
MspA1I CMGCKG 1 cut(s) 306
MspI CCGG 2 cut(s) 285, 509
MspR9I CCNGG 2 cut(s) 234, 285
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 4 cut(s) 306, 312, 315, 324
NciI CCSGG 1 cut(s) 285
NdeII GATC 4 cut(s) 118, 430, 450, 466
NlaIII CATG 2 cut(s) 322, 377
NlaIV GGNNCC 3 cut(s) 161, 202, 238
NmuCI GTSAC 3 cut(s) 25, 34, 167
PagI TCATGA 1 cut(s) 373
PceI AGGCCT 1 cut(s) 275
PfeI GAWTC 1 cut(s) 370
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 7 cut(s) 101, 302, 305, 308, 317, 411, 482
PshAI GACNNNNGTC 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 232
PspEI GGTNACC 1 cut(s) 25
PspGI CCWGG 1 cut(s) 232
PspN4I GGNNCC 3 cut(s) 161, 202, 238
PspPI GGNCC 1 cut(s) 201
PstI CTGCAG 1 cut(s) 189
PsuI RGATCY 1 cut(s) 430
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 7 cut(s) 100, 301, 304, 307, 316, 410, 481
Sau3AI GATC 4 cut(s) 118, 430, 450, 466
Sau96I GGNCC 1 cut(s) 201
ScrFI CCNGG 2 cut(s) 234, 285
SetI ASST 5 cut(s) 9, 27, 165, 219, 366
SfaNI GCATC 2 cut(s) 57, 101
SfcI CTRYAG 1 cut(s) 185
SinI GGWCC 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
SseBI AGGCCT 1 cut(s) 275
SsiI CCGC 3 cut(s) 288, 300, 306
SspMI CTAG 1 cut(s) 150
StuI AGGCCT 1 cut(s) 275
StyD4I CCNGG 2 cut(s) 232, 283
StyI CCWWGG 1 cut(s) 21
TaiI ACGT 2 cut(s) 9, 366
TauI GCSGC 2 cut(s) 303, 309
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 3 cut(s) 25, 34, 167
TseI GCWGC 5 cut(s) 99, 303, 315, 409, 480
Tsp45I GTSAC 3 cut(s) 25, 34, 167
TspDTI ATGAA 1 cut(s) 362
TspRI CASTG 1 cut(s) 385
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 201
XagI CCTNNNNNAGG 1 cut(s) 244
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.