Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0016104

Physical Location & Seq

Genomic Coordinates Forward (+)

19654034 .. 19657048

Gene Structure 3015 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g15750.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGTCCACCACCACCATGCAGCTGAAGCTGAACCACCTCTGCTTCGTTTTGCCACCAGAGGACTTCGACGATATCGAGCCACCGGACCAAGAAAACCACCACAATCCCACCACGGAGGTGTTGGAGATCAGAAAGAATCATACCCGTCATGAATGCATGAGTCATTTTCGTGCTTTTGTCAAAGACTCTCTCTGCAGGCTCTACGACCTGAAATGGATCAATCTGTGTCACCATGACACGAGAAAGAGTCGACACCGAAACCATTCTGGGGTGTTTCAGGACATGGATGGGATAGAGCTCAAGGACAAGGTCGGAGGAGACAACCCGAGGATCTTCAGCTTCTCGGAGCTTTATATTGGTTCCAATGGTTTCAGTGAGGAACAAGTTTTGGGGAGTGGAGGGTTTGGGAAAGTTTACAGAGCTGTTCTGCCTAGTGATGGAACTCTTGTTGCTGTGAAGTGCTTGGTGGAGAGAGGAGAGCGGTTCGAGAAGACCTTTGTGGCGGAGTTGCTGGCTGTGGCTCATCTACGTCACCGGAATCTTGTCCGGCTAAGAGGGTGGTGTGTTCATGAGAAACAGCTCTTTTTGGTTTATGACTACATGCCAAACCTTAGCCTTGACCGTATACTTTTCAGGAGACCTGAAAATATGGGGTCTCCAGCAGCAGTGCCTCTCAATTGGGAGCGTAGAAGGAACATAATCGGTGGCTTGGCAGCAGCGCTTTACTATCTCCACGAACAGTTGGAAACTCAGATCATTCACCGGGATGTCAAAACGAGTAATGTGATGCTTGATTCACATTTCAATGCTCGGCTAGGCGACTTTGGCTTGGCAAGGTGGCTGGAACATGAGATTGAGTACGAGATAAAGACACCATCTACGCAAAACCACCAGTTTCGGCTAGCAGAAACGACTAAAATTGGTGGAACAATCGGGTATCTGCCACCGGAAAGCTTCCAGAAACGAAGCGTGGCTACTGCGAAGTCTGATGTTTTCAGCTTTGGGATTGTTGTTATGGAGGTTGTGTCAGGTAGAAGGGCAGTGGATCTCGCTTGTCCAGATGATCAGATCATATTGCTAGACTGGATCCGGAAGCTGTCTGATGAAGGGAAGCTTTTACAAGCAGGGGACAGTAGGATTCCAGACGGGTCTTATCAGCTTGTTGACATGGAGTATCAGTTGCATCTTGCACTTCTATGCACACTTCAAAATCCGCAGCTGCGACCAAACATGAAATGGGTTGTGGAAGCACATTCTGGCAACATTTATGGCAAACTGCCGGGCCTCCCATCGTTTCAGTGCCAGCCTCTCTACATCTCTCTGTCCTCTGCCAGTAATTATAGCACCAGGTACACAATAGCCTCCACAACAGCAACTTTTGCATCATCTATTCTATCGAACTATGTGACAGCCACCGGAGAAACAATATATGCAACTGCTGAAAATGGAAGCAGTCGAAGCAGTGAGGTCAGCTCGACTGAAAGCTTCCCACAGAAGAAAGCAACATTTCCTCTTGTTGAAACTCCAAGAGAAATATCCTACATGGAGATCATATCTGCGACAAATAATTTCGCAGATTCTCAGAGGGTAGCAGAGCTGGACTTTGGGACAGCCTACCAGGGTTTTCTCAATAACGGCCATCATGTCCTGGTAAAGCGGCTTGGCATGAAGACTTGCCCTGCATTGCGGGCACGATTCTCAAATGAACTTCAAAACTTGGGAAGGCTCCGTCACAGGAACTTGGTTCAGCTCCGTGGTTGGTGCACTGAGCAAGGAGAAATGCTAGTTGTCTATGATTATTTAGCTGATCGCCTTCTAAGCCACCTACTGTTTAGTAAAGATTACAGATTTGGCAATTCAATCCTGCAATGGCGTCACAGATGCAACATTATCAAATCGCTTGCTTCTGCGATTCTCTATCTCCACGAGGAATGGGACGAACAAGTTATTCACAGAAACATTACCTCTTCTGCTGTTATTCTTGATCCAGACATGAATCCAAGGCTCAGTTCTTTTGCCCTTGCTGAATTCTTGACACGGAACGACCATGGGCATCATGCAGTTACTGACACAAGCAAATCAGCTCGAGGGATTTTCGGTTACATGTCACCTGAATGCATGGAATCTGGAGAAGTGAATACAATGACTGACATATACAGCTTTGGTGTAGTAATGCTTGAGGTCATCACCGGACAGATGGCAGTTGATTTTAGGCGGCCTGAGGTGCTACTGGTCAGGAGGGTTCATGAATTTGAGGCACGCACAAAGACTTATAAGGAAATGGCGGATATTAGGTTGAATGGAGTTTACAATCAGAAGGAACTGATGAGACTGTTCAAACTAGGACTTGCTTGTACACGCTCTAATCCTCAATCAAGACCGAGCATGAGGCAGGTTGTTCGAATACTTGATGGGAATGACAACTGCTTGGCAGAACTACGCAGAAAAGAGGAAAGTAGGGAAGAATGGAGACGAGTGAATGATTCTGCTCTGTCACTAATTAAGAGGATCCAAGCTCTAGGAATACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0008150 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0043207 GO:0044464 GO:0046677 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071944 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

844

Amino Acids

96.26

Weight (kDa)

7.4

Isoelectric Point (pI)

49.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	125 - 348	1.4e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	127 - 345	3.1e-35	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	530 - 803	1.5e-31	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	534 - 800	1.1e-24	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32800
fragaria_vesca	FvH4_4g14630 FvH4_4g15750 FvH4_5g04690
malus_domestica	MD04G1008600.v1.1
prunus_persica	Prupe.1G007200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0415351
rosa_laevigata	RLG00000008076
rosa_multiflora	Rmu_sc0013726.1_g000001 Rmu_sc0028326.1_g000002
rosa_roxburghii	Rroxscaffold_5G00359130
rosa_rugosa	Rorug04G0133800
rosa_samantha	Rh4BG192800 Rh4DG191900
rosa_wichuraiana	Rw4G016520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2274
Acc36I	ACCTGC	1 cut(s)	2383
AccBSI	CCGCTC	1 cut(s)	481
AccI	GTMKAC	2 cut(s)	250, 625
AccIII	TCCGGA	1 cut(s)	1089
AciI	CCGC	7 cut(s)	481, 503, 1214, 1657, 1687, 2215, 2285
AclWI	GGATC	8 cut(s)	224, 338, 1053, 1081, 1094, 1979, 2503, 2516
AcoI	YGGCCR	1 cut(s)	1636
AcsI	RAATTY	2 cut(s)	2027, 2249
AcuI	CTGAAG	2 cut(s)	44, 319
AcyI	GRCGYC	1 cut(s)	1873
AfaI	GTAC	3 cut(s)	860, 1352, 2356
AfeI	AGCGCT	1 cut(s)	720
AfiI	CCNNNNNNNGG	2 cut(s)	268, 437
AflIII	ACRYGT	1 cut(s)	2103
AgsI	TTSAA	7 cut(s)	805, 1208, 1520, 1712, 1860, 2299, 2338
AjnI	CCWGG	3 cut(s)	1346, 1617, 1647
AjuI	GAANNNNNNNTTGG	2 cut(s)	1993, 2025
AleI	CACNNNNGTG	1 cut(s)	116
Alw21I	GWGCWC	2 cut(s)	300, 1766
Alw26I	GTCTC	5 cut(s)	312, 631, 660, 2323, 2464
Alw44I	GTGCAC	1 cut(s)	1762
AlwI	GGATC	8 cut(s)	224, 338, 1053, 1081, 1094, 1979, 2503, 2516
AlwNI	CAGNNNCTG	1 cut(s)	2066
Ama87I	CYCGRG	2 cut(s)	325, 2085
Aor13HI	TCCGGA	1 cut(s)	1089
Aor51HI	AGCGCT	1 cut(s)	720
AoxI	GGCC	3 cut(s)	1282, 1636, 2216
ApaLI	GTGCAC	1 cut(s)	1762
ApeKI	GCWGC	6 cut(s)	19, 662, 713, 716, 1216, 1219
ApoI	RAATTY	2 cut(s)	2027, 2249
Asp700I	GAANNNNTTC	1 cut(s)	262
AspLEI	GCGC	1 cut(s)	721
AspS9I	GGNCC	2 cut(s)	85, 1282
AsuC2I	CCSGG	2 cut(s)	764, 1281
AsuHPI	GGTGA	5 cut(s)	221, 524, 752, 2100, 2179
AsuII	TTCGAA	1 cut(s)	2401
AsuNHI	GCTAGC	1 cut(s)	901
AvaI	CYCGRG	2 cut(s)	325, 2085
AvaII	GGWCC	1 cut(s)	85
AxyI	CCTNAGG	1 cut(s)	2220
BaeGI	GKGCMC	2 cut(s)	1693, 1766
BamHI	GGATCC	2 cut(s)	1086, 2508
BanII	GRGCYC	1 cut(s)	300
BarI	GAAGNNNNNNTAC	2 cut(s)	958, 990
BauI	CACGAG	2 cut(s)	238, 1925
BbsI	GAAGAC	2 cut(s)	497, 1676
Bbv12I	GWGCWC	2 cut(s)	300, 1766
BbvI	GCAGC	6 cut(s)	31, 674, 725, 728, 1206, 1228
BccI	CCATC	7 cut(s)	281, 431, 883, 1297, 1647, 2191, 2405
BceAI	ACGGC	1 cut(s)	1651
BcgI	CGANNNNNNTGC	2 cut(s)	2381, 2415
BciT130I	CCWGG	3 cut(s)	1348, 1619, 1649
BclI	TGATCA	1 cut(s)	1063
BcnI	CCSGG	2 cut(s)	764, 1281
BcoDI	GTCTC	5 cut(s)	312, 631, 660, 2323, 2464
BfaI	CTAG	7 cut(s)	432, 815, 902, 1079, 1784, 2342, 2519
BfmI	CTRYAG	1 cut(s)	193
BfoI	RGCGCY	1 cut(s)	722
BfuAI	ACCTGC	1 cut(s)	2383
BisI	GCNGC	8 cut(s)	20, 663, 714, 717, 1217, 1220, 1658, 2216
BlsI	GCNGC	8 cut(s)	21, 664, 715, 718, 1218, 1221, 1659, 2217
Bme1390I	CCNGG	5 cut(s)	764, 1281, 1348, 1619, 1649
Bme18I	GGWCC	1 cut(s)	85
BmeT110I	CYCGRG	2 cut(s)	325, 2085
BmgT120I	GGNCC	2 cut(s)	85, 1282
BmiI	GGNNCC	4 cut(s)	361, 1088, 1727, 2510
BmrFI	CCNGG	5 cut(s)	764, 1281, 1348, 1619, 1649
BmsI	GCATC	5 cut(s)	777, 1192, 1391, 1871, 2062
BmtI	GCTAGC	1 cut(s)	905
BpiI	GAAGAC	2 cut(s)	497, 1676
BplI	GAGNNNNNCTC	2 cut(s)	1457, 1489
BpmI	CTGGAG	2 cut(s)	642, 2148
Bpu10I	CCTNAGC	1 cut(s)	611
Bpu14I	TTCGAA	1 cut(s)	2401
BpuEI	CTTGAG	2 cut(s)	284, 2198
BpuMI	CCSGG	2 cut(s)	764, 1281
BsaHI	GRCGYC	1 cut(s)	1873
BsaI	GGTCTC	2 cut(s)	631, 660
BsaJI	CCNNGG	6 cut(s)	111, 326, 1618, 1753, 2000, 2047
BsaWI	WCCGGW	6 cut(s)	82, 534, 946, 1089, 1415, 2189
BsaXI	ACNNNNNCTCC	4 cut(s)	1010, 1040, 1163, 1193
Bsc4I	CCNNNNNNNGG	2 cut(s)	268, 437
Bse1I	ACTGG	4 cut(s)	892, 1088, 1332, 2235
Bse21I	CCTNAGG	1 cut(s)	2220
Bse3DI	GCAATG	2 cut(s)	1682, 1874
BseAI	TCCGGA	1 cut(s)	1089
BseBI	CCWGG	3 cut(s)	1348, 1619, 1649
BseDI	CCNNGG	6 cut(s)	111, 326, 1618, 1753, 2000, 2047
BseGI	GGATG	2 cut(s)	292, 772
BseLI	CCNNNNNNNGG	2 cut(s)	268, 437
BseMI	GCAATG	2 cut(s)	1682, 1874
BseMII	CTCAG	5 cut(s)	764, 1595, 1758, 2020, 2211
BseNI	ACTGG	4 cut(s)	892, 1088, 1332, 2235
BseRI	GAGGAG	2 cut(s)	330, 489
BseSI	GKGCMC	2 cut(s)	1693, 1766
BseXI	GCAGC	6 cut(s)	31, 674, 725, 728, 1206, 1228
BshFI	GGCC	3 cut(s)	1284, 1638, 2218
BsiHKAI	GWGCWC	2 cut(s)	300, 1766
BsiHKCI	CYCGRG	2 cut(s)	325, 2085
BsiSI	CCGG	9 cut(s)	83, 535, 547, 763, 947, 1090, 1280, 1416, 2190
BslFI	GGGAC	3 cut(s)	1142, 1621, 1949
BslI	CCNNNNNNNGG	2 cut(s)	268, 437
BsmAI	GTCTC	5 cut(s)	312, 631, 660, 2323, 2464
BsmBI	CGTCTC	1 cut(s)	2464
BsmFI	GGGAC	3 cut(s)	1142, 1621, 1949
BsmI	GAATGC	2 cut(s)	158, 2120
BsnI	GGCC	3 cut(s)	1284, 1638, 2218
Bso31I	GGTCTC	2 cut(s)	631, 660
BsoBI	CYCGRG	2 cut(s)	325, 2085
Bsp119I	TTCGAA	1 cut(s)	2401
Bsp1286I	GDGCHC	3 cut(s)	300, 1693, 1766
Bsp13I	TCCGGA	1 cut(s)	1089
Bsp1407I	TGTACA	1 cut(s)	2354
Bsp19I	CCATGG	1 cut(s)	2047
BspACI	CCGC	7 cut(s)	481, 503, 1214, 1657, 1687, 2215, 2285
BspANI	GGCC	3 cut(s)	1284, 1638, 2218
BspCNI	CTCAG	5 cut(s)	763, 1594, 1759, 2019, 2212
BspEI	TCCGGA	1 cut(s)	1089
BspHI	TCATGA	3 cut(s)	148, 568, 2245
BspLI	GGNNCC	4 cut(s)	361, 1088, 1727, 2510
BspMAI	CTGCAG	1 cut(s)	197
BspMI	ACCTGC	1 cut(s)	2383
BspOI	GCTAGC	1 cut(s)	905
BspPI	GGATC	8 cut(s)	224, 338, 1053, 1081, 1094, 1979, 2503, 2516
BspT104I	TTCGAA	1 cut(s)	2401
BspTNI	GGTCTC	2 cut(s)	631, 660
BsrBI	CCGCTC	1 cut(s)	481
BsrDI	GCAATG	2 cut(s)	1682, 1874
BsrGI	TGTACA	1 cut(s)	2354
BsrI	ACTGG	4 cut(s)	892, 1088, 1332, 2235
BssECI	CCNNGG	6 cut(s)	111, 326, 1618, 1753, 2000, 2047
BssNAI	GTATAC	1 cut(s)	626
BssNI	GRCGYC	1 cut(s)	1873
BssSI	CACGAG	2 cut(s)	238, 1925
BssT1I	CCWWGG	2 cut(s)	2000, 2047
Bst1107I	GTATAC	1 cut(s)	626
Bst2BI	CACGAG	2 cut(s)	238, 1925
Bst2UI	CCWGG	3 cut(s)	1348, 1619, 1649
Bst4CI	ACNGT	5 cut(s)	623, 741, 1133, 1830, 2334
Bst6I	CTCTTC	1 cut(s)	1972
BstACI	GRCGYC	1 cut(s)	1873
BstAPI	GCANNNNNTGC	1 cut(s)	1379
BstAUI	TGTACA	1 cut(s)	2354
BstBI	TTCGAA	1 cut(s)	2401
BstC8I	GCNNGC	7 cut(s)	197, 513, 903, 1304, 1689, 1902, 2260
BstDEI	CTNAG	8 cut(s)	551, 611, 750, 1581, 1767, 1817, 2006, 2220
BstDSI	CCRYGG	3 cut(s)	111, 1753, 2047
BstF5I	GGATG	2 cut(s)	292, 772
BstH2I	RGCGCY	1 cut(s)	722
BstHHI	GCGC	1 cut(s)	721
BstMAI	GTCTC	5 cut(s)	312, 631, 660, 2323, 2464
BstMWI	GCNNNNNNNGC	7 cut(s)	25, 825, 1379, 1458, 1688, 1818, 2224
BstNI	CCWGG	3 cut(s)	1348, 1619, 1649
BstNSI	RCATGY	2 cut(s)	604, 2107
BstSCI	CCNGG	5 cut(s)	762, 1279, 1346, 1617, 1647
BstSFI	CTRYAG	1 cut(s)	193
BstSLI	GKGCMC	2 cut(s)	1693, 1766
BstV1I	GCAGC	6 cut(s)	31, 674, 725, 728, 1206, 1228
BstV2I	GAAGAC	2 cut(s)	497, 1676
BstX2I	RGATCY	4 cut(s)	330, 1045, 1086, 2508
BstYI	RGATCY	4 cut(s)	330, 1045, 1086, 2508
BstZ17I	GTATAC	1 cut(s)	626
Bsu36I	CCTNAGG	1 cut(s)	2220
BsuRI	GGCC	3 cut(s)	1284, 1638, 2218
BtgI	CCRYGG	3 cut(s)	111, 1753, 2047
BtsCI	GGATG	2 cut(s)	292, 772
BtsI	GCAGTG	3 cut(s)	672, 1047, 1468
BtsIMutI	CAGTG	6 cut(s)	379, 672, 1047, 1304, 1468, 1764
BveI	ACCTGC	1 cut(s)	2383
Cac8I	GCNNGC	7 cut(s)	197, 513, 903, 1304, 1689, 1902, 2260
CaiI	CAGNNNCTG	1 cut(s)	2066
CciI	TCATGA	3 cut(s)	148, 568, 2245
CfoI	GCGC	1 cut(s)	721
Cfr13I	GGNCC	2 cut(s)	85, 1282
CseI	GACGC	1 cut(s)	1862
CsiI	ACCWGGT	1 cut(s)	1346
Csp6I	GTAC	3 cut(s)	859, 1351, 2355
CviQI	GTAC	3 cut(s)	859, 1351, 2355
DdeI	CTNAG	8 cut(s)	551, 611, 750, 1581, 1767, 1817, 2006, 2220
EaeI	YGGCCR	1 cut(s)	1636
Eam1104I	CTCTTC	1 cut(s)	1972
EarI	CTCTTC	1 cut(s)	1972
EciI	GGCGGA	2 cut(s)	518, 2300
Ecl136II	GAGCTC	1 cut(s)	298
Eco130I	CCWWGG	2 cut(s)	2000, 2047
Eco24I	GRGCYC	1 cut(s)	300
Eco31I	GGTCTC	2 cut(s)	631, 660
Eco32I	GATATC	1 cut(s)	73
Eco47I	GGWCC	1 cut(s)	85
Eco47III	AGCGCT	1 cut(s)	720
Eco53kI	GAGCTC	1 cut(s)	298
Eco57I	CTGAAG	2 cut(s)	44, 319
Eco81I	CCTNAGG	1 cut(s)	2220
Eco88I	CYCGRG	2 cut(s)	325, 2085
EcoICRI	GAGCTC	1 cut(s)	298
EcoRI	GAATTC	1 cut(s)	2027
EcoRII	CCWGG	3 cut(s)	1346, 1617, 1647
EcoRV	GATATC	1 cut(s)	73
EcoT14I	CCWWGG	2 cut(s)	2000, 2047
EcoT22I	ATGCAT	2 cut(s)	158, 2120
EcoT38I	GRGCYC	1 cut(s)	300
ErhI	CCWWGG	2 cut(s)	2000, 2047
Esp3I	CGTCTC	1 cut(s)	2464
FalI	AAGNNNNNCTT	2 cut(s)	1098, 1130
FaqI	GGGAC	3 cut(s)	1142, 1621, 1949
FauI	CCCGC	1 cut(s)	1680
FbaI	TGATCA	1 cut(s)	1063
FblI	GTMKAC	2 cut(s)	250, 625
Fnu4HI	GCNGC	8 cut(s)	20, 663, 714, 717, 1217, 1220, 1658, 2216
FokI	GGATG	2 cut(s)	299, 779
FriOI	GRGCYC	1 cut(s)	300
Fsp4HI	GCNGC	8 cut(s)	20, 663, 714, 717, 1217, 1220, 1658, 2216
FspBI	CTAG	7 cut(s)	432, 815, 902, 1079, 1784, 2342, 2519
GlaI	GCGC	1 cut(s)	720
GluI	GCNGC	8 cut(s)	20, 663, 714, 717, 1217, 1220, 1658, 2216
GsuI	CTGGAG	2 cut(s)	642, 2148
HaeII	RGCGCY	1 cut(s)	722
HaeIII	GGCC	3 cut(s)	1284, 1638, 2218
HapII	CCGG	9 cut(s)	83, 535, 547, 763, 947, 1090, 1280, 1416, 2190
HgaI	GACGC	1 cut(s)	1862
HhaI	GCGC	1 cut(s)	721
Hin1I	GRCGYC	1 cut(s)	1873
Hin6I	GCGC	1 cut(s)	719
HinP1I	GCGC	1 cut(s)	719
HincII	GTYRAC	2 cut(s)	251, 1165
HindII	GTYRAC	2 cut(s)	251, 1165
HindIII	AAGCTT	3 cut(s)	952, 1112, 1483
HpaII	CCGG	9 cut(s)	83, 535, 547, 763, 947, 1090, 1280, 1416, 2190
HphI	GGTGA	5 cut(s)	221, 524, 752, 2100, 2179
Hpy166II	GTNNAC	9 cut(s)	6, 251, 415, 626, 1165, 1353, 1764, 2308, 2357
Hpy188I	TCNGA	9 cut(s)	131, 314, 346, 753, 988, 1068, 1102, 1584, 2316
Hpy8I	GTNNAC	9 cut(s)	6, 251, 415, 626, 1165, 1353, 1764, 2308, 2357
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	6 cut(s)	684, 1029, 1100, 1716, 1823, 2311
HpyCH4III	ACNGT	5 cut(s)	623, 741, 1133, 1830, 2334
HpyCH4IV	ACGT	1 cut(s)	529
HpyF10VI	GCNNNNNNNGC	7 cut(s)	25, 825, 1379, 1458, 1688, 1818, 2224
HpyF3I	CTNAG	8 cut(s)	551, 611, 750, 1581, 1767, 1817, 2006, 2220
HpySE526I	ACGT	1 cut(s)	529
Hsp92I	GRCGYC	1 cut(s)	1873
HspAI	GCGC	1 cut(s)	719
Kpn2I	TCCGGA	1 cut(s)	1089
Ksp22I	TGATCA	1 cut(s)	1063
LmnI	GCTCC	4 cut(s)	346, 682, 1731, 1755
Lsp1109I	GCAGC	6 cut(s)	31, 674, 725, 728, 1206, 1228
LweI	GCATC	5 cut(s)	777, 1192, 1391, 1871, 2062
MabI	ACCWGGT	1 cut(s)	1346
MaeI	CTAG	7 cut(s)	432, 815, 902, 1079, 1784, 2342, 2519
MaeII	ACGT	1 cut(s)	529
MaeIII	GTNAC	9 cut(s)	227, 530, 1405, 1730, 1874, 2062, 2099, 2106, 2493
MbiI	CCGCTC	1 cut(s)	481
MboII	GAAGA	6 cut(s)	325, 502, 1507, 1681, 1959, 2474
MfeI	CAATTG	1 cut(s)	676
MflI	RGATCY	4 cut(s)	330, 1045, 1086, 2508
MhlI	GDGCHC	3 cut(s)	300, 1693, 1766
MluCI	AATT	8 cut(s)	676, 918, 1336, 1567, 1855, 2027, 2249, 2499
MlyI	GAGTC	3 cut(s)	169, 179, 256
MmeI	TCCRAC	3 cut(s)	102, 292, 723
Mph1103I	ATGCAT	2 cut(s)	158, 2120
MroI	TCCGGA	1 cut(s)	1089
MroXI	GAANNNNTTC	1 cut(s)	262
MseI	TTAA	1 cut(s)	2502
MslI	CAYNNNNRTG	7 cut(s)	14, 116, 168, 765, 804, 1195, 2113
MspA1I	CMGCKG	2 cut(s)	22, 1219
MspI	CCGG	9 cut(s)	83, 535, 547, 763, 947, 1090, 1280, 1416, 2190
MspR9I	CCNGG	5 cut(s)	764, 1281, 1348, 1619, 1649
MunI	CAATTG	1 cut(s)	676
Mva1269I	GAATGC	2 cut(s)	158, 2120
MvaI	CCWGG	3 cut(s)	1348, 1619, 1649
MwoI	GCNNNNNNNGC	7 cut(s)	25, 825, 1379, 1458, 1688, 1818, 2224
NciI	CCSGG	2 cut(s)	764, 1281
NcoI	CCATGG	1 cut(s)	2047
NheI	GCTAGC	1 cut(s)	901
NlaIV	GGNNCC	4 cut(s)	361, 1088, 1727, 2510
NmeAIII	GCCGAG	1 cut(s)	790
NmuCI	GTSAC	7 cut(s)	227, 530, 1405, 1730, 1874, 2106, 2493
NsiI	ATGCAT	2 cut(s)	158, 2120
NspI	RCATGY	2 cut(s)	604, 2107
NspV	TTCGAA	1 cut(s)	2401
OliI	CACNNNNGTG	1 cut(s)	116
PaeR7I	CTCGAG	1 cut(s)	2085
PagI	TCATGA	3 cut(s)	148, 568, 2245
PciI	ACATGT	1 cut(s)	2103
PcsI	WCGNNNNNNNCGW	1 cut(s)	72
PctI	GAATGC	2 cut(s)	158, 2120
PdmI	GAANNNNTTC	1 cut(s)	262
PflFI	GACNNNGTC	1 cut(s)	308
PkrI	GCNGC	8 cut(s)	21, 664, 715, 718, 1218, 1221, 1659, 2217
PleI	GAGTC	3 cut(s)	168, 179, 255
PpsI	GAGTC	3 cut(s)	168, 179, 255
PscI	ACATGT	1 cut(s)	2103
PsiI	TTATAA	1 cut(s)	2274
Psp124BI	GAGCTC	1 cut(s)	300
Psp6I	CCWGG	3 cut(s)	1346, 1617, 1647
PspGI	CCWGG	3 cut(s)	1346, 1617, 1647
PspN4I	GGNNCC	4 cut(s)	361, 1088, 1727, 2510
PspPI	GGNCC	2 cut(s)	85, 1282
PspXI	VCTCGAGB	1 cut(s)	2085
PstI	CTGCAG	1 cut(s)	197
PstNI	CAGNNNCTG	1 cut(s)	2066
PsuI	RGATCY	4 cut(s)	330, 1045, 1086, 2508
PsyI	GACNNNGTC	1 cut(s)	308
PvuII	CAGCTG	2 cut(s)	22, 1219
RsaI	GTAC	3 cut(s)	860, 1352, 2356
RsaNI	GTAC	3 cut(s)	859, 1351, 2355
RseI	CAYNNNNRTG	7 cut(s)	14, 116, 168, 765, 804, 1195, 2113
SacI	GAGCTC	1 cut(s)	300
SalI	GTCGAC	1 cut(s)	249
SaqAI	TTAA	1 cut(s)	2502
SatI	GCNGC	8 cut(s)	20, 663, 714, 717, 1217, 1220, 1658, 2216
Sau96I	GGNCC	2 cut(s)	85, 1282
SchI	GAGTC	3 cut(s)	169, 179, 256
ScrFI	CCNGG	5 cut(s)	764, 1281, 1348, 1619, 1649
SduI	GDGCHC	3 cut(s)	300, 1693, 1766
SexAI	ACCWGGT	1 cut(s)	1346
SfaNI	GCATC	5 cut(s)	777, 1192, 1391, 1871, 2062
SfcI	CTRYAG	1 cut(s)	193
Sfr274I	CTCGAG	1 cut(s)	2085
SfuI	TTCGAA	1 cut(s)	2401
SinI	GGWCC	1 cut(s)	85
SlaI	CTCGAG	1 cut(s)	2085
SmiMI	CAYNNNNRTG	7 cut(s)	14, 116, 168, 765, 804, 1195, 2113
SmlI	CTYRAG	3 cut(s)	299, 2085, 2177
SmoI	CTYRAG	3 cut(s)	299, 2085, 2177
Sse9I	AATT	8 cut(s)	676, 918, 1336, 1567, 1855, 2027, 2249, 2499
SsiI	CCGC	7 cut(s)	481, 503, 1214, 1657, 1687, 2215, 2285
SspMI	CTAG	7 cut(s)	432, 815, 902, 1079, 1784, 2342, 2519
SstI	GAGCTC	1 cut(s)	300
StyD4I	CCNGG	5 cut(s)	762, 1279, 1346, 1617, 1647
StyI	CCWWGG	2 cut(s)	2000, 2047
TaaI	ACNGT	5 cut(s)	623, 741, 1133, 1830, 2334
TaiI	ACGT	1 cut(s)	532
TaqI	TCGA	9 cut(s)	66, 75, 250, 486, 1397, 1456, 1475, 2086, 2401
TaqII	GACCGA	1 cut(s)	2395
TasI	AATT	8 cut(s)	676, 918, 1336, 1567, 1855, 2027, 2249, 2499
TatI	WGTACW	1 cut(s)	2354
TauI	GCSGC	2 cut(s)	1660, 2218
Tru1I	TTAA	1 cut(s)	2502
Tru9I	TTAA	1 cut(s)	2502
TscAI	CASTG	6 cut(s)	379, 672, 1047, 1304, 1468, 1771
TseFI	GTSAC	7 cut(s)	227, 530, 1405, 1730, 1874, 2106, 2493
TseI	GCWGC	6 cut(s)	19, 662, 713, 716, 1216, 1219
Tsp45I	GTSAC	7 cut(s)	227, 530, 1405, 1730, 1874, 2106, 2493
TspDTI	ATGAA	9 cut(s)	165, 557, 1119, 1247, 1682, 1719, 2009, 2234, 2262
TspGWI	ACGGA	4 cut(s)	128, 1718, 1742, 2053
TspRI	CASTG	6 cut(s)	379, 672, 1047, 1304, 1468, 1771
Tth111I	GACNNNGTC	1 cut(s)	308
VneI	GTGCAC	1 cut(s)	1762
VpaK11BI	GGWCC	1 cut(s)	85
XapI	RAATTY	2 cut(s)	2027, 2249
XceI	RCATGY	2 cut(s)	604, 2107
XcmI	CCANNNNNNNNNTGG	2 cut(s)	118, 1233
XhoI	CTCGAG	1 cut(s)	2085
XmiI	GTMKAC	2 cut(s)	250, 625
XmnI	GAANNNNTTC	1 cut(s)	262
XspI	CTAG	7 cut(s)	432, 815, 902, 1079, 1784, 2342, 2519
Zsp2I	ATGCAT	2 cut(s)	158, 2120

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.