Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0016104

Physical Location & Seq

Genomic Coordinates Reverse (-)

982034 .. 984529

Gene Structure 2496 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1008600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGCAGCTGAATCGTCTTTGCTTCGTACTACCGGAAGACTACAAAGAAATGGAGCCGGTGGATCACCAAAACCTCCACAAACCTACCAAGGAAGACATAAAAAAAAATCCACCACATCGTCACTGCACGAGTCATATTCGTGGTCTCATCAAGGATTCACTATGCAGGCTATATGATTTGATATGGATCAACTTCTGTCACCATGACGCTACGCAACGGAAGCAACACTCGGGCGCGTTTCAAGACATGGACGGGATACAGCTCAAGGACAAGGTCGGAGGAGACAATCCGAGGATCTTTAGCTTTTCCGAGCTTTATATTGGCTCGAACGGGTTCAGCGAGGATCAAGTTCTTGGAAGTGGAGGTTTCGGGAGAGTGTACAGAGCGGTTTTACCGAGTGATGGAACTGTGGTTGCTGTGAAATGCTTGGTGGAGAGAGGAGAGCAGTTTGAGAAGACTTTTGCGGCGGAGTTATTGGCCGTGGCTCACCTCCGTCATAGGAACCTTGTCCGGCTGAGAGGATGGTGTGTTCATGAAAACCAGCTGTTTTTGGTTTATGACTTCATGCCTAACCTTAGCCTCGATCGCGTACTTTTCAGGAGACCTGAAAATATGCGGTCGGTCTCTCTCAGTTGGCAGCGTAGAGCGAACATAGTCAGCGGGTTGGCAGCAGCGCTTTTCTATCTTCACGAACAGTTGGAGACTCAGATCATTCATAGGGATGTTAAGACGAGTAACGTGATGCTTGATTCCCATTACAATGCTCGGCTTGGTGACTTTGGCTTGGCACGGTGGCTAGAACATGAACTTGAGTCCCAAGCACGGACGCCGTCAATGAAAAATGTTCAGTTTCGGATGGCAGAAACCACGAGAATTGGTGGAACAATTGGGTATTTGCCGCCCGAAAGCTTCCAAAAAAGAAGCGTGGCTACTGCGAAATCTGATGTTTTCAGCTTTGGAATTGTTGTGCTTGAGGTGGTGTCAGGTAGGCGGGCAGTAGACCTCGCTTGCTCAGATGATCAGATCATATTGCTCGACTGGATTCGAAGGCTATCTGATGAAGGGAAGCTTTTACAAGCAGGGGACAATAGGATTCCAGATGATTCTTACCAGCTTTCTGATATGGAGCATTTGTTTCATGTTGCTCTTCTTTGCACACTTCACAGCCCGCAATCGCGACCAAACATGAAATGGGTTGTGGATGCACTTTCCGGCAACATTCATGGCAAGTTGCCGGACCTCCCATCGTTTCAGTGCCACCCTTTGTACATCTCTTTATCCTCTCCCTCGAGTGCTGGCACGAACAATGGCAGCAGCGCCAACAGCAGGTACACAATTGGCACCACCAACACAACCACATCTACTACATCAACTTATGTCACAGCCAATGCAGACACCATCTTTGCAAGTGCTGAAAACGGAAGCAGTGATGTCAGCTCTTCTCAAAGCTTCCGTCTGAAGAAATCAACATTTCCCATGGTTGAAACTCCGCAAGAAATATCCTACAAGGAAATTGTATCTGCTACGAATAATTTCGCAGACTCTCAGCGGGTAGCGGAGCTGGACTTTGGAACAGCCTACCAGGGCTTTCTCAACAACCGCCATCATGTCCTTGTGAAGCGGCTTGGCATGAAAACATGCCCAGCACTGAGGGCGCGATTTTCAGATGAACTTCAGAACCTAGGAAGGCTCCGCCACCGAAACTTGGTACAGCTCCGTGGATGGTGCACGGAACAAGGAGAGATGCTTGTTGTCTACGATTACTTGGCAGATCGCCTTCTGAGTAACCTACTTTTCCATCACGACAATAGATTCGTTAATTGTATCCTGCAATGGCGTCACAGATACAACATTATAAAATCGCTTGCTTCCGCCATTCTTTATCTTCACGAGGAATGGGACGAACAAGTGATTCACAGAAATATTACCTCCTCGGCTGTTATTCTTGATCCAGACATGAACCCGAGGCTGACTTCATTTGCCCTTGCTGAATTCTTGACACGAAATGACCACGGGCATCATGCAAGTACCAACGCAACCAAATCAGTTCGTGGAATTTTCGGTTACATGTCACCAGAATATATGTTATCTGGGGAAGCAACACCAATGGCTGATATATATAGCTTCGGGGTGGTAATGCTTGAGGTTGTTAGCGGACAGCTGGCAATCGACTTCAGGCGGCCTGAGGTGCTACTGGTGAAGAGGGTTCATGAATTTGAGGTGCGCAGAAGACCATTCGAGGAGCTGGCAGACATCAGATTGAATGGAGCGTACAATCACAAAGAACTGATGAGACTGATTAAGCTAGGAATTGACTGTACACGCTCCAATCCTCGATCAAGACCAACCATACGGCAGATTGTAAGGATACTTGATGGTGATGACAAATGCTTCATGCAAGAAGGGAAAAGGGCGGAAATTGTCGAAGATTGGAGACGAATGAATGGTTGTTCCTTGTCATTGATTAGGAGTATCCAAGCTCTAGGAATACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0008150 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0043207 GO:0044464 GO:0046677 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071944 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

832

Amino Acids

94.58

Weight (kDa)

8.1

Isoelectric Point (pI)

44.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	113 - 334	3.4e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	115 - 401	1.4e-35	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	518 - 791	2.2e-28	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	522 - 788	1.5e-24	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32800
fragaria_vesca	FvH4_4g14630 FvH4_4g15750 FvH4_5g04690
malus_domestica	MD04G1008600.v1.1
prunus_persica	Prupe.1G007200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0415351
rosa_laevigata	RLG00000008076
rosa_multiflora	Rmu_sc0013726.1_g000001 Rmu_sc0028326.1_g000002
rosa_roxburghii	Rroxscaffold_5G00359130
rosa_rugosa	Rorug04G0133800
rosa_samantha	Rh4BG192800 Rh4DG191900
rosa_wichuraiana	Rw4G016520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1856
Acc16I	TGCGCA	1 cut(s)	2225
Acc36I	ACCTGC	1 cut(s)	1317
AccB1I	GGYRCC	1 cut(s)	1340
AccBSI	CCGCTC	1 cut(s)	386
AccI	GTMKAC	2 cut(s)	999, 1755
AccII	CGCG	4 cut(s)	236, 588, 1177, 1657
AclWI	GGATC	5 cut(s)	69, 194, 302, 351, 1943
AcoI	YGGCCR	1 cut(s)	477
AcsI	RAATTY	3 cut(s)	1991, 2055, 2213
AcuI	CTGAAG	3 cut(s)	1478, 1658, 2158
AcyI	GRCGYC	2 cut(s)	827, 1837
AfaI	GTAC	9 cut(s)	27, 380, 591, 1268, 1331, 1710, 2029, 2273, 2320
AfeI	AGCGCT	1 cut(s)	675
AfiI	CCNNNNNNNGG	4 cut(s)	401, 822, 1326, 1705
AflIII	ACRYGT	1 cut(s)	2067
AgsI	TTSAA	3 cut(s)	242, 1484, 2263
AjnI	CCWGG	1 cut(s)	1581
AloI	GAACNNNNNNTCC	2 cut(s)	513, 545
Alw21I	GWGCWC	1 cut(s)	1730
Alw26I	GTCTC	7 cut(s)	149, 276, 595, 628, 695, 2287, 2428
Alw44I	GTGCAC	1 cut(s)	1726
AlwI	GGATC	5 cut(s)	69, 194, 302, 351, 1943
Ama87I	CYCGRG	3 cut(s)	229, 1288, 1963
Aor51HI	AGCGCT	1 cut(s)	675
AoxI	GGCC	2 cut(s)	477, 2180
ApaLI	GTGCAC	1 cut(s)	1726
ApeKI	GCWGC	6 cut(s)	4, 637, 668, 671, 1311, 1314
ApoI	RAATTY	3 cut(s)	1991, 2055, 2213
ArsI	GACNNNNNNTTYG	6 cut(s)	1385, 1417, 1438, 1470, 1796, 1828
Asp700I	GAANNNNTTC	2 cut(s)	332, 1532
AspA2I	CCTAGG	1 cut(s)	1681
AspLEI	GCGC	5 cut(s)	236, 676, 1319, 1657, 2226
AspS9I	GGNCC	1 cut(s)	1237
AsuHPI	GGTGA	7 cut(s)	56, 191, 479, 785, 2064, 2209, 2390
AsuII	TTCGAA	1 cut(s)	1045
AvaI	CYCGRG	3 cut(s)	229, 1288, 1963
AvaII	GGWCC	1 cut(s)	1237
AvrII	CCTAGG	1 cut(s)	1681
AxyI	CCTNAGG	1 cut(s)	2184
BaeGI	GKGCMC	1 cut(s)	1730
BanI	GGYRCC	1 cut(s)	1340
BarI	GAAGNNNNNNTAC	2 cut(s)	913, 945
BauI	CACGAG	3 cut(s)	127, 868, 1889
BbsI	GAAGAC	4 cut(s)	42, 99, 461, 2236
Bbv12I	GWGCWC	1 cut(s)	1730
BbvI	GCAGC	6 cut(s)	16, 649, 680, 683, 1323, 1326
BccI	CCATC	9 cut(s)	395, 516, 850, 1252, 1406, 1611, 1716, 1806, 2369
BceAI	ACGGC	3 cut(s)	464, 814, 2369
BcgI	CGANNNNNNTGC	1 cut(s)	27
BciT130I	CCWGG	1 cut(s)	1583
BciVI	GTATCC	4 cut(s)	249, 1835, 2361, 2483
BclI	TGATCA	1 cut(s)	1018
BcoDI	GTCTC	7 cut(s)	149, 276, 595, 628, 695, 2287, 2428
BfaI	CTAG	4 cut(s)	797, 1682, 2306, 2483
BfoI	RGCGCY	2 cut(s)	677, 1320
BfuAI	ACCTGC	1 cut(s)	1317
BfuI	GTATCC	4 cut(s)	249, 1835, 2361, 2483
BlnI	CCTAGG	1 cut(s)	1681
Bme1390I	CCNGG	1 cut(s)	1583
Bme18I	GGWCC	1 cut(s)	1237
BmeT110I	CYCGRG	3 cut(s)	229, 1288, 1963
BmgT120I	GGNCC	1 cut(s)	1237
BmiI	GGNNCC	4 cut(s)	54, 503, 1342, 1691
BmrFI	CCNGG	1 cut(s)	1583
BmsI	GCATC	4 cut(s)	732, 1192, 1734, 2026
BpiI	GAAGAC	4 cut(s)	42, 99, 461, 2236
Bpu10I	CCTNAGC	1 cut(s)	575
Bpu14I	TTCGAA	1 cut(s)	1045
BpuEI	CTTGAG	4 cut(s)	248, 830, 992, 2162
BsaBI	GATNNNNATC	1 cut(s)	185
BsaHI	GRCGYC	2 cut(s)	827, 1837
BsaI	GGTCTC	3 cut(s)	149, 595, 628
BsaWI	WCCGGW	1 cut(s)	31
BsaXI	ACNNNNNCTCC	2 cut(s)	1118, 1148
Bsc4I	CCNNNNNNNGG	4 cut(s)	401, 822, 1326, 1705
Bse118I	RCCGGY	1 cut(s)	55
Bse1I	ACTGG	2 cut(s)	1043, 2199
Bse21I	CCTNAGG	1 cut(s)	2184
Bse3DI	GCAATG	1 cut(s)	1838
Bse8I	GATNNNNATC	1 cut(s)	185
BseBI	CCWGG	1 cut(s)	1583
BseGI	GGATG	5 cut(s)	527, 727, 861, 1207, 1727
BseJI	GATNNNNATC	1 cut(s)	185
BseLI	CCNNNNNNNGG	4 cut(s)	401, 822, 1326, 1705
BseMI	GCAATG	1 cut(s)	1838
BseMII	CTCAG	8 cut(s)	506, 643, 719, 1026, 1559, 1640, 1772, 2175
BseNI	ACTGG	2 cut(s)	1043, 2199
BseRI	GAGGAG	4 cut(s)	294, 453, 1921, 2255
BseSI	GKGCMC	1 cut(s)	1730
BseXI	GCAGC	6 cut(s)	16, 649, 680, 683, 1323, 1326
BseYI	CCCAGC	1 cut(s)	1642
BsgI	GTGCAG	1 cut(s)	109
Bsh1236I	CGCG	4 cut(s)	236, 588, 1177, 1657
Bsh1285I	CGRYCG	2 cut(s)	586, 620
BshFI	GGCC	2 cut(s)	479, 2182
BshNI	GGYRCC	1 cut(s)	1340
BsiEI	CGRYCG	2 cut(s)	586, 620
BsiHKAI	GWGCWC	1 cut(s)	1730
BsiHKCI	CYCGRG	3 cut(s)	229, 1288, 1963
BsiSI	CCGG	5 cut(s)	32, 56, 511, 1212, 1235
BslFI	GGGAC	3 cut(s)	799, 1097, 1913
BslI	CCNNNNNNNGG	4 cut(s)	401, 822, 1326, 1705
BsmAI	GTCTC	7 cut(s)	149, 276, 595, 628, 695, 2287, 2428
BsmBI	CGTCTC	1 cut(s)	2428
BsmFI	GGGAC	3 cut(s)	799, 1097, 1913
BsnI	GGCC	2 cut(s)	479, 2182
Bso31I	GGTCTC	3 cut(s)	149, 595, 628
BsoBI	CYCGRG	3 cut(s)	229, 1288, 1963
Bsp119I	TTCGAA	1 cut(s)	1045
Bsp1286I	GDGCHC	1 cut(s)	1730
Bsp1407I	TGTACA	3 cut(s)	378, 1266, 2318
Bsp19I	CCATGG	1 cut(s)	1476
Bsp68I	TCGCGA	1 cut(s)	1177
BspANI	GGCC	2 cut(s)	479, 2182
BspCNI	CTCAG	8 cut(s)	507, 642, 718, 1025, 1558, 1641, 1773, 2176
BspFNI	CGCG	4 cut(s)	236, 588, 1177, 1657
BspHI	TCATGA	2 cut(s)	532, 2209
BspLI	GGNNCC	4 cut(s)	54, 503, 1342, 1691
BspMI	ACCTGC	1 cut(s)	1317
BspPI	GGATC	5 cut(s)	69, 194, 302, 351, 1943
BspQI	GCTCTTC	2 cut(s)	1152, 1444
BspT104I	TTCGAA	1 cut(s)	1045
BspT107I	GGYRCC	1 cut(s)	1340
BspTNI	GGTCTC	3 cut(s)	149, 595, 628
BsrBI	CCGCTC	1 cut(s)	386
BsrDI	GCAATG	1 cut(s)	1838
BsrFI	RCCGGY	1 cut(s)	55
BsrGI	TGTACA	3 cut(s)	378, 1266, 2318
BsrI	ACTGG	2 cut(s)	1043, 2199
BssAI	RCCGGY	1 cut(s)	55
BssNI	GRCGYC	2 cut(s)	827, 1837
BssSI	CACGAG	3 cut(s)	127, 868, 1889
BssT1I	CCWWGG	3 cut(s)	87, 1476, 1681
Bst2BI	CACGAG	3 cut(s)	127, 868, 1889
Bst2UI	CCWGG	1 cut(s)	1583
Bst4CI	ACNGT	4 cut(s)	409, 696, 792, 2318
Bst6I	CTCTTC	3 cut(s)	1152, 1444, 2195
BstACI	GRCGYC	2 cut(s)	827, 1837
BstAUI	TGTACA	3 cut(s)	378, 1266, 2318
BstBI	TTCGAA	1 cut(s)	1045
BstC8I	GCNNGC	8 cut(s)	167, 993, 1009, 1169, 1297, 1866, 2163, 2247
BstDEI	CTNAG	9 cut(s)	515, 575, 629, 705, 1012, 1545, 1649, 1781, 2184
BstDSI	CCRYGG	4 cut(s)	480, 1476, 1717, 2011
BstF5I	GGATG	5 cut(s)	527, 727, 861, 1207, 1727
BstFNI	CGCG	4 cut(s)	236, 588, 1177, 1657
BstH2I	RGCGCY	2 cut(s)	677, 1320
BstHHI	GCGC	5 cut(s)	236, 676, 1319, 1657, 2226
BstMAI	GTCTC	7 cut(s)	149, 276, 595, 628, 695, 2287, 2428
BstMCI	CGRYCG	2 cut(s)	586, 620
BstMWI	GCNNNNNNNGC	5 cut(s)	220, 585, 1323, 1652, 2188
BstNI	CCWGG	1 cut(s)	1583
BstNSI	RCATGY	2 cut(s)	1641, 2071
BstSCI	CCNGG	1 cut(s)	1581
BstSLI	GKGCMC	1 cut(s)	1730
BstUI	CGCG	4 cut(s)	236, 588, 1177, 1657
BstV1I	GCAGC	6 cut(s)	16, 649, 680, 683, 1323, 1326
BstV2I	GAAGAC	4 cut(s)	42, 99, 461, 2236
BstX2I	RGATCY	1 cut(s)	294
BstYI	RGATCY	1 cut(s)	294
Bsu36I	CCTNAGG	1 cut(s)	2184
BsuI	GTATCC	4 cut(s)	249, 1835, 2361, 2483
BsuRI	GGCC	2 cut(s)	479, 2182
BtgI	CCRYGG	4 cut(s)	480, 1476, 1717, 2011
BtsCI	GGATG	5 cut(s)	527, 727, 861, 1207, 1727
BtsI	GCAGTG	2 cut(s)	121, 1432
BtsIMutI	CAGTG	4 cut(s)	121, 1259, 1432, 1646
BtuMI	TCGCGA	1 cut(s)	1177
BveI	ACCTGC	1 cut(s)	1317
Cac8I	GCNNGC	8 cut(s)	167, 993, 1009, 1169, 1297, 1866, 2163, 2247
CciI	TCATGA	2 cut(s)	532, 2209
CfoI	GCGC	5 cut(s)	236, 676, 1319, 1657, 2226
Cfr10I	RCCGGY	1 cut(s)	55
Cfr13I	GGNCC	1 cut(s)	1237
CseI	GACGC	3 cut(s)	215, 835, 1826
Csp6I	GTAC	9 cut(s)	26, 379, 590, 1267, 1330, 1709, 2028, 2272, 2319
CspCI	CAANNNNNGTGG	2 cut(s)	856, 891
CviQI	GTAC	9 cut(s)	26, 379, 590, 1267, 1330, 1709, 2028, 2272, 2319
DdeI	CTNAG	9 cut(s)	515, 575, 629, 705, 1012, 1545, 1649, 1781, 2184
EaeI	YGGCCR	1 cut(s)	477
Eam1104I	CTCTTC	3 cut(s)	1152, 1444, 2195
EarI	CTCTTC	3 cut(s)	1152, 1444, 2195
EciI	GGCGGA	4 cut(s)	482, 1682, 1861, 2429
Eco130I	CCWWGG	3 cut(s)	87, 1476, 1681
Eco31I	GGTCTC	3 cut(s)	149, 595, 628
Eco47I	GGWCC	1 cut(s)	1237
Eco47III	AGCGCT	1 cut(s)	675
Eco57I	CTGAAG	3 cut(s)	1478, 1658, 2158
Eco81I	CCTNAGG	1 cut(s)	2184
Eco88I	CYCGRG	3 cut(s)	229, 1288, 1963
EcoRI	GAATTC	1 cut(s)	1991
EcoRII	CCWGG	1 cut(s)	1581
EcoT14I	CCWWGG	3 cut(s)	87, 1476, 1681
ErhI	CCWWGG	3 cut(s)	87, 1476, 1681
Esp3I	CGTCTC	1 cut(s)	2428
FalI	AAGNNNNNCTT	2 cut(s)	1053, 1085
FaqI	GGGAC	3 cut(s)	799, 1097, 1913
FauI	CCCGC	4 cut(s)	653, 984, 1176, 1542
FbaI	TGATCA	1 cut(s)	1018
FblI	GTMKAC	2 cut(s)	999, 1755
FokI	GGATG	5 cut(s)	534, 734, 868, 1214, 1734
FspBI	CTAG	4 cut(s)	797, 1682, 2306, 2483
FspI	TGCGCA	1 cut(s)	2225
GlaI	GCGC	5 cut(s)	235, 675, 1318, 1656, 2225
GsaI	CCCAGC	1 cut(s)	1646
HaeII	RGCGCY	2 cut(s)	677, 1320
HaeIII	GGCC	2 cut(s)	479, 2182
HapII	CCGG	5 cut(s)	32, 56, 511, 1212, 1235
HgaI	GACGC	3 cut(s)	215, 835, 1826
HhaI	GCGC	5 cut(s)	236, 676, 1319, 1657, 2226
Hin1I	GRCGYC	2 cut(s)	827, 1837
Hin6I	GCGC	5 cut(s)	234, 674, 1317, 1655, 2224
HinP1I	GCGC	5 cut(s)	234, 674, 1317, 1655, 2224
HindIII	AAGCTT	3 cut(s)	907, 1067, 1447
HpaII	CCGG	5 cut(s)	32, 56, 511, 1212, 1235
HphI	GGTGA	7 cut(s)	56, 191, 479, 785, 2064, 2209, 2390
Hpy166II	GTNNAC	6 cut(s)	379, 1000, 1332, 1728, 1756, 2321
Hpy8I	GTNNAC	6 cut(s)	379, 1000, 1332, 1728, 1756, 2321
HpyAV	CCTTC	5 cut(s)	1041, 1055, 1680, 1787, 2396
HpyCH4III	ACNGT	4 cut(s)	409, 696, 792, 2318
HpyCH4IV	ACGT	1 cut(s)	738
HpyF10VI	GCNNNNNNNGC	5 cut(s)	220, 585, 1323, 1652, 2188
HpyF3I	CTNAG	9 cut(s)	515, 575, 629, 705, 1012, 1545, 1649, 1781, 2184
HpySE526I	ACGT	1 cut(s)	738
Hsp92I	GRCGYC	2 cut(s)	827, 1837
HspAI	GCGC	5 cut(s)	234, 674, 1317, 1655, 2224
Ksp22I	TGATCA	1 cut(s)	1018
LguI	GCTCTTC	2 cut(s)	1152, 1444
LmnI	GCTCC	8 cut(s)	52, 1126, 1558, 1695, 1719, 2242, 2267, 2330
Lsp1109I	GCAGC	6 cut(s)	16, 649, 680, 683, 1323, 1326
LweI	GCATC	4 cut(s)	732, 1192, 1734, 2026
MaeI	CTAG	4 cut(s)	797, 1682, 2306, 2483
MaeII	ACGT	1 cut(s)	738
MaeIII	GTNAC	9 cut(s)	119, 197, 734, 773, 1378, 1784, 1838, 2063, 2070
MbiI	CCGCTC	1 cut(s)	386
MfeI	CAATTG	2 cut(s)	885, 1335
MflI	RGATCY	1 cut(s)	294
MhlI	GDGCHC	1 cut(s)	1730
MlyI	GAGTC	4 cut(s)	139, 697, 821, 1535
MmeI	TCCRAC	2 cut(s)	256, 678
MroXI	GAANNNNTTC	2 cut(s)	332, 1532
MseI	TTAA	3 cut(s)	726, 1818, 2301
MslI	CAYNNNNRTG	3 cut(s)	138, 720, 759
MspA1I	CMGCKG	5 cut(s)	7, 544, 660, 1549, 2161
MspI	CCGG	5 cut(s)	32, 56, 511, 1212, 1235
MspR9I	CCNGG	1 cut(s)	1583
MunI	CAATTG	2 cut(s)	885, 1335
MvaI	CCWGG	1 cut(s)	1583
MvnI	CGCG	4 cut(s)	236, 588, 1177, 1657
MwoI	GCNNNNNNNGC	5 cut(s)	220, 585, 1323, 1652, 2188
NcoI	CCATGG	1 cut(s)	1476
NlaIV	GGNNCC	4 cut(s)	54, 503, 1342, 1691
NmeAIII	GCCGAG	2 cut(s)	745, 1913
NmuCI	GTSAC	6 cut(s)	119, 197, 773, 1378, 1838, 2070
NruI	TCGCGA	1 cut(s)	1177
NsbI	TGCGCA	1 cut(s)	2225
NspI	RCATGY	2 cut(s)	1641, 2071
NspV	TTCGAA	1 cut(s)	1045
PaeR7I	CTCGAG	1 cut(s)	1288
PagI	TCATGA	2 cut(s)	532, 2209
PciI	ACATGT	1 cut(s)	2067
PciSI	GCTCTTC	2 cut(s)	1152, 1444
PcsI	WCGNNNNNNNCGW	1 cut(s)	336
PdmI	GAANNNNTTC	2 cut(s)	332, 1532
PfeI	GAWTC	8 cut(s)	10, 155, 749, 1042, 1093, 1103, 1812, 1912
PflFI	GACNNNGTC	2 cut(s)	272, 829
Ple19I	CGATCG	1 cut(s)	586
PleI	GAGTC	4 cut(s)	138, 697, 820, 1535
PpsI	GAGTC	4 cut(s)	138, 697, 820, 1535
PscI	ACATGT	1 cut(s)	2067
PsiI	TTATAA	1 cut(s)	1856
Psp6I	CCWGG	1 cut(s)	1581
PspFI	CCCAGC	1 cut(s)	1642
PspGI	CCWGG	1 cut(s)	1581
PspN4I	GGNNCC	4 cut(s)	54, 503, 1342, 1691
PspPI	GGNCC	1 cut(s)	1237
PspXI	VCTCGAGB	1 cut(s)	1288
PsuI	RGATCY	1 cut(s)	294
PsyI	GACNNNGTC	2 cut(s)	272, 829
PvuI	CGATCG	1 cut(s)	586
PvuII	CAGCTG	3 cut(s)	7, 544, 2161
RruI	TCGCGA	1 cut(s)	1177
RsaI	GTAC	9 cut(s)	27, 380, 591, 1268, 1331, 1710, 2029, 2273, 2320
RsaNI	GTAC	9 cut(s)	26, 379, 590, 1267, 1330, 1709, 2028, 2272, 2319
RseI	CAYNNNNRTG	3 cut(s)	138, 720, 759
SapI	GCTCTTC	2 cut(s)	1152, 1444
SaqAI	TTAA	3 cut(s)	726, 1818, 2301
Sau96I	GGNCC	1 cut(s)	1237
SchI	GAGTC	4 cut(s)	139, 697, 821, 1535
ScrFI	CCNGG	1 cut(s)	1583
SduI	GDGCHC	1 cut(s)	1730
SfaNI	GCATC	4 cut(s)	732, 1192, 1734, 2026
Sfr274I	CTCGAG	1 cut(s)	1288
SfuI	TTCGAA	1 cut(s)	1045
SinI	GGWCC	1 cut(s)	1237
SlaI	CTCGAG	1 cut(s)	1288
SmiMI	CAYNNNNRTG	3 cut(s)	138, 720, 759
SmlI	CTYRAG	5 cut(s)	263, 809, 971, 1288, 2141
SmoI	CTYRAG	5 cut(s)	263, 809, 971, 1288, 2141
SspI	AATATT	1 cut(s)	1924
SspMI	CTAG	4 cut(s)	797, 1682, 2306, 2483
StyD4I	CCNGG	1 cut(s)	1581
StyI	CCWWGG	3 cut(s)	87, 1476, 1681
TaaI	ACNGT	4 cut(s)	409, 696, 792, 2318
TaiI	ACGT	1 cut(s)	741
TaqI	TCGA	9 cut(s)	326, 582, 1035, 1045, 1289, 2169, 2238, 2335, 2424
TaqII	GACCGA	1 cut(s)	610
TatI	WGTACW	3 cut(s)	378, 1266, 2318
TauI	GCSGC	4 cut(s)	467, 901, 1624, 2182
TfiI	GAWTC	8 cut(s)	10, 155, 749, 1042, 1093, 1103, 1812, 1912
Tru1I	TTAA	3 cut(s)	726, 1818, 2301
Tru9I	TTAA	3 cut(s)	726, 1818, 2301
TscAI	CASTG	4 cut(s)	128, 1259, 1432, 1653
TseFI	GTSAC	6 cut(s)	119, 197, 773, 1378, 1838, 2070
TseI	GCWGC	6 cut(s)	4, 637, 668, 671, 1311, 1314
Tsp45I	GTSAC	6 cut(s)	119, 197, 773, 1378, 1838, 2070
TspGWI	ACGGA	7 cut(s)	232, 482, 838, 1434, 1442, 1706, 1745
TspRI	CASTG	4 cut(s)	128, 1259, 1432, 1653
Tth111I	GACNNNGTC	2 cut(s)	272, 829
VneI	GTGCAC	1 cut(s)	1726
VpaK11BI	GGWCC	1 cut(s)	1237
XapI	RAATTY	3 cut(s)	1991, 2055, 2213
XceI	RCATGY	2 cut(s)	1641, 2071
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1188
XhoI	CTCGAG	1 cut(s)	1288
XmaJI	CCTAGG	1 cut(s)	1681
XmiI	GTMKAC	2 cut(s)	999, 1755
XmnI	GAANNNNTTC	2 cut(s)	332, 1532
XspI	CTAG	4 cut(s)	797, 1682, 2306, 2483

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.