Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0016104

Physical Location & Seq

Genomic Coordinates Forward (+)

39186843 .. 39189365

Gene Structure 2523 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00359130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGTCCACCACCACCACCATGCAGCTGAACCACCTCTGCTTCATTTTGCCACCAGACTGCGACGAAATCGAGCCACCGGACCACGAAAACCAGCAAAAACCCACCAAGGAGGTGTTAGAGGTCAGAAAGAATCACACCCGTCGCGAATGCCTGAGTCATGTTCGTGCTTTCGTCAAAGAGTCACTCTGCAGGCTCTACCACCTGAAATGGATCAGTCTCTGTCACCATGACACGAGAAAAAACCGAGACCAGCACCATTCTGGGGTGTTTCAGGACATGGATGGGATACAACTCAAGGACAAGGTCGGAGGAGACAACCCGAGGATCTTCAGCTTCACGGAGCTTTATATTGGTTCGAATGGTTTCAGTGAGGATCAACTTTTAGGGAGTGGAGGTTTTGGGAAAGTGTACAGAGCGGTTCTGCCTAGTGATGGAACTGTTGTTGCTGTGAAGTGCTTGGTCGAGAGAGGAGAGCGGTTCGAGAAGACTTTTGTGGCGGAGTTGGTTGCGGTGGCTCACCTTCGTCACCGGAATCTTGTCCGGCTAAGGGGGTGGTGTGTTCATGAAAAAGAGCTGTTTTTGGTTTATGACTACATGCCGAACCTCAGCCTTGACCGCATACTTTTCAGGAAACCTGAGAATATAGGGTCTCCGACATCAGTGCCTCTCAATTGGGAGCGTAGAAGGAACATAATCGGTGGCTTGGCAGCGGCGCTTTACTATCTTCACGAACAGTTGGAAACTCAAATCATTCACCGGGATGTCAAGACGAGTAATGTGATGCTTGATTCCCATTTTAATGCTCGGCTAGGCGACTTTGGCTTGGCACGGTGGCTGGAACATGAAATTGAGCACGAGATAAAGACACCATCGATGCAAAACCACCAGTTTCGGCTAGCAGAAACGACCAAAATTGGTGGAACAATCGGGTATTTGCCACCTGAAAGCTTCCAGAAACGAAGCGTGGCTACTGCAAAATCTGATGTTTTCAGCTTTGGGATTGTTGTTCTGGAGGTGGTGTCGGGTAGAAGGGCAGTAGATCTCACTTGTCCAGATGATCAGATCATATTGCTGGACTGGATCCGGAAGCTGGCTGATGAAGGGAAGCTTTTAAAAGCAAGGGACAATAGGATTCCAGACGGGTCTTATCAGCTTCTTGACATGGAGTATCACTTGCATCTTGCACTTCTTTGTACACTTCAAAATCCACAATCGCGACCAAACATGAAATGGGTTGTGGAAGCACTTTCTGGCAACATCCATGGCAAATTGCCGAGCCTCCCATCCTTTCAGTGCCATCCTCTCTACATCTCTCTATCCTCTGCCAGCAATACTAGCACAAACACCAGGTACACCATAGCCACCACCACTGCATCTCTTGCATCATCTATTCTATCAACCTATGTCACAGCCACTGGAGAAACAATATATGCAACTGCTGACAATGGAAGCAGTGAGGTCAGCTCGTGTGAAAGCTTCGTACATAAGGAAGCAACATTTCCCCTGGTTGAAACTCCACGAGAAATATCCTACATGGAAATTGTATCTGCCACAAATAATTTCGCAGATTCTCAAAGGGTAGCAGAGCTGGACTTTGGGACCGCCTACCAGGGTTTTCTCAACAACCGCCATCATGTCCTGGTAAAGCGGCTTGGAATGAAGACTTGCCCTGCATTGCGGGCACGATTCTCAAATGAACTTAAAAACTTGGGAAGGCTCCGCCACCGAAACTTGGTTCAGCTCCGTGGATGGTGCACTGAGCAAGGAGAAATGCTAGTTGTCTATGATTACTTGGCTGATCGCCTTCTAAGCCACCTACTTTTTCGTGGAGACTACAGATTTGGCCATTCAATCCTGCAATGGCGTCATAGATACAACATTATCAAATCACTTGCTTCTGCGATTCTTTATCTCCACGAGGAATGGGACGAACAAGTTATTCACAGAAACATTACCTCTTCTGCTGTTATTCTCGATCCAGACATGAACCCAAGACTCAGTTCTTTCGCTCTTGCTGAATTCTTGACACGGAACGACCATGGGCATCATGCAGTTGCTGCCACAAGCAAATCTGCTCGAGGGATTTTTGGTTACATGTCACCTGAATGTATGGAATCTGGAGAAGCAACCACAATGACTGACATATACAGCTTTGGTGTAGTAATACTTGAGGTTATAACCGGACAGATGGCAGTTGACTTTCGGCGGCCTGAGGTGCTACTGGTCAAGAGGGTCCGTGAATTTGGGGCACGGAAAAAGACTTTAGAGGATATGGCAGATTTTAGGTTGAATGGAGTGTACAATCAGAAGGAACTGTTGAGACTGTTTAAACTAGGAATTGCTTGTACACGCTCCAATCCTCAACTAAGACCGAGCATGAGGCAGATTGTAAGGATACTTGATGGGAATGACAAATGCTTGACGGAAATATGCAAAAAGGACGAAAGTGGGGAAGAATGGAGACAAGTGAATGATTCTGCTTTGTCGCTAATTAAGAGGATCCAAGCTCTAGGAATACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0008150 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0043207 GO:0044464 GO:0046677 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071944 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

840

Amino Acids

95.63

Weight (kDa)

8.05

Isoelectric Point (pI)

42.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	123 - 348	5.5e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	125 - 414	5.2e-35	Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr	PF07714	527 - 800	1.3e-29	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	531 - 797	4.7e-24	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32800
fragaria_vesca	FvH4_4g14630 FvH4_4g15750 FvH4_5g04690
malus_domestica	MD04G1008600.v1.1
prunus_persica	Prupe.1G007200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0415351
rosa_laevigata	RLG00000008076
rosa_multiflora	Rmu_sc0013726.1_g000001 Rmu_sc0028326.1_g000002
rosa_roxburghii	Rroxscaffold_5G00359130
rosa_rugosa	Rorug04G0133800
rosa_samantha	Rh4BG192800 Rh4DG191900
rosa_wichuraiana	Rw4G016520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2177
AccBSI	CCGCTC	2 cut(s)	416, 475
AccII	CGCG	2 cut(s)	144, 1216
AccIII	TCCGGA	1 cut(s)	1083
AclWI	GGATC	8 cut(s)	218, 332, 381, 1075, 1088, 1970, 2494, 2507
AcoI	YGGCCR	1 cut(s)	1843
AcsI	RAATTY	2 cut(s)	2018, 2240
AcuI	CTGAAG	1 cut(s)	313
AcyI	GRCGYC	1 cut(s)	1864
AfaI	GTAC	6 cut(s)	410, 1195, 1352, 1482, 2300, 2347
AfiI	CCNNNNNNNGG	5 cut(s)	262, 431, 547, 1090, 1732
AflIII	ACRYGT	1 cut(s)	2094
AgsI	TTSAA	4 cut(s)	1202, 1511, 1851, 2290
AjnI	CCWGG	4 cut(s)	1346, 1503, 1608, 1638
AjuI	GAANNNNNNNTTGG	2 cut(s)	1984, 2016
Alw21I	GWGCWC	2 cut(s)	855, 1757
Alw26I	GTCTC	7 cut(s)	221, 240, 306, 654, 1824, 2314, 2455
Alw44I	GTGCAC	1 cut(s)	1753
AlwI	GGATC	8 cut(s)	218, 332, 381, 1075, 1088, 1970, 2494, 2507
AlwNI	CAGNNNCTG	3 cut(s)	219, 1415, 2057
Ama87I	CYCGRG	2 cut(s)	319, 2076
Aor13HI	TCCGGA	1 cut(s)	1083
AoxI	GGCC	2 cut(s)	1843, 2207
ApaLI	GTGCAC	1 cut(s)	1753
ApeKI	GCWGC	3 cut(s)	22, 707, 2057
ApoI	RAATTY	2 cut(s)	2018, 2240
ArsI	GACNNNNNNTTYG	2 cut(s)	1823, 1855
Asp700I	GAANNNNTTC	1 cut(s)	362
AspLEI	GCGC	1 cut(s)	715
AspS9I	GGNCC	3 cut(s)	79, 1599, 2233
AsuC2I	CCSGG	1 cut(s)	758
AsuHPI	GGTGA	5 cut(s)	215, 509, 518, 746, 2091
AsuII	TTCGAA	1 cut(s)	356
AsuNHI	GCTAGC	1 cut(s)	895
AvaI	CYCGRG	2 cut(s)	319, 2076
AvaII	GGWCC	3 cut(s)	79, 1599, 2233
AxyI	CCTNAGG	1 cut(s)	2211
BaeGI	GKGCMC	3 cut(s)	1684, 1757, 2251
BalI	TGGCCA	1 cut(s)	1845
BamHI	GGATCC	2 cut(s)	1080, 2499
BarI	GAAGNNNNNNTAC	2 cut(s)	952, 984
BauI	CACGAG	5 cut(s)	232, 854, 1465, 1518, 1916
BbsI	GAAGAC	2 cut(s)	491, 1667
Bbv12I	GWGCWC	2 cut(s)	855, 1757
BbvCI	CCTCAGC	1 cut(s)	605
BbvI	GCAGC	3 cut(s)	34, 719, 2044
BccI	CCATC	9 cut(s)	275, 425, 877, 1291, 1305, 1638, 1743, 2182, 2396
BcgI	CGANNNNNNTGC	2 cut(s)	643, 677
BciT130I	CCWGG	4 cut(s)	1348, 1505, 1610, 1640
BciVI	GTATCC	2 cut(s)	279, 2388
BclI	TGATCA	1 cut(s)	1057
BcnI	CCSGG	1 cut(s)	758
BcoDI	GTCTC	7 cut(s)	221, 240, 306, 654, 1824, 2314, 2455
BfaI	CTAG	7 cut(s)	426, 809, 896, 1335, 1775, 2333, 2510
BfmI	CTRYAG	2 cut(s)	187, 1834
BfoI	RGCGCY	1 cut(s)	716
BfuI	GTATCC	2 cut(s)	279, 2388
BglII	AGATCT	1 cut(s)	1039
BisI	GCNGC	6 cut(s)	23, 708, 711, 1649, 2058, 2207
BlsI	GCNGC	6 cut(s)	24, 709, 712, 1650, 2059, 2208
Bme1390I	CCNGG	5 cut(s)	758, 1348, 1505, 1610, 1640
Bme18I	GGWCC	3 cut(s)	79, 1599, 2233
BmeT110I	CYCGRG	2 cut(s)	319, 2076
BmgT120I	GGNCC	3 cut(s)	79, 1599, 2233
BmiI	GGNNCC	5 cut(s)	1082, 1600, 1718, 2234, 2501
BmrFI	CCNGG	5 cut(s)	758, 1348, 1505, 1610, 1640
BmsI	GCATC	6 cut(s)	771, 864, 1186, 1382, 1391, 2053
BmtI	GCTAGC	1 cut(s)	899
BpiI	GAAGAC	2 cut(s)	491, 1667
BplI	GAGNNNNNCTC	2 cut(s)	1448, 1480
BpmI	CTGGAG	3 cut(s)	1031, 1437, 2139
Bpu10I	CCTNAGC	2 cut(s)	545, 605
Bpu14I	TTCGAA	1 cut(s)	356
BpuEI	CTTGAG	2 cut(s)	278, 2189
BpuMI	CCSGG	1 cut(s)	758
Bsa29I	ATCGAT	1 cut(s)	872
BsaHI	GRCGYC	1 cut(s)	1864
BsaI	GGTCTC	2 cut(s)	240, 654
BsaJI	CCNNGG	7 cut(s)	105, 320, 1261, 1503, 1609, 1744, 2038
BsaWI	WCCGGW	4 cut(s)	76, 528, 1083, 2180
BsaXI	ACNNNNNCTCC	4 cut(s)	379, 409, 1004, 1034
Bsc4I	CCNNNNNNNGG	5 cut(s)	262, 431, 547, 1090, 1732
Bse1I	ACTGG	4 cut(s)	886, 1082, 1420, 2226
Bse21I	CCTNAGG	1 cut(s)	2211
Bse3DI	GCAATG	2 cut(s)	1673, 1865
BseAI	TCCGGA	1 cut(s)	1083
BseBI	CCWGG	4 cut(s)	1348, 1505, 1610, 1640
BseCI	ATCGAT	1 cut(s)	872
BseDI	CCNNGG	7 cut(s)	105, 320, 1261, 1503, 1609, 1744, 2038
BseGI	GGATG	6 cut(s)	286, 766, 1257, 1283, 1297, 1754
BseLI	CCNNNNNNNGG	5 cut(s)	262, 431, 547, 1090, 1732
BseMI	GCAATG	2 cut(s)	1673, 1865
BseMII	CTCAG	6 cut(s)	143, 619, 627, 1749, 2011, 2202
BseNI	ACTGG	4 cut(s)	886, 1082, 1420, 2226
BseRI	GAGGAG	2 cut(s)	324, 483
BseSI	GKGCMC	3 cut(s)	1684, 1757, 2251
BseXI	GCAGC	3 cut(s)	34, 719, 2044
Bsh1236I	CGCG	2 cut(s)	144, 1216
BshFI	GGCC	2 cut(s)	1845, 2209
BshVI	ATCGAT	1 cut(s)	872
BsiHKAI	GWGCWC	2 cut(s)	855, 1757
BsiHKCI	CYCGRG	2 cut(s)	319, 2076
BsiSI	CCGG	6 cut(s)	77, 529, 541, 757, 1084, 2181
BslFI	GGGAC	3 cut(s)	1136, 1612, 1940
BslI	CCNNNNNNNGG	5 cut(s)	262, 431, 547, 1090, 1732
BsmAI	GTCTC	7 cut(s)	221, 240, 306, 654, 1824, 2314, 2455
BsmFI	GGGAC	3 cut(s)	1136, 1612, 1940
BsmI	GAATGC	1 cut(s)	152
BsnI	GGCC	2 cut(s)	1845, 2209
Bso31I	GGTCTC	2 cut(s)	240, 654
BsoBI	CYCGRG	2 cut(s)	319, 2076
Bsp119I	TTCGAA	1 cut(s)	356
Bsp1286I	GDGCHC	4 cut(s)	855, 1684, 1757, 2251
Bsp13I	TCCGGA	1 cut(s)	1083
Bsp1407I	TGTACA	4 cut(s)	408, 1193, 2298, 2345
Bsp19I	CCATGG	2 cut(s)	1261, 2038
Bsp68I	TCGCGA	2 cut(s)	144, 1216
BspANI	GGCC	2 cut(s)	1845, 2209
BspCNI	CTCAG	6 cut(s)	144, 618, 628, 1750, 2010, 2203
BspDI	ATCGAT	1 cut(s)	872
BspEI	TCCGGA	1 cut(s)	1083
BspFNI	CGCG	2 cut(s)	144, 1216
BspHI	TCATGA	1 cut(s)	562
BspLI	GGNNCC	5 cut(s)	1082, 1600, 1718, 2234, 2501
BspMAI	CTGCAG	1 cut(s)	191
BspOI	GCTAGC	1 cut(s)	899
BspPI	GGATC	8 cut(s)	218, 332, 381, 1075, 1088, 1970, 2494, 2507
BspT104I	TTCGAA	1 cut(s)	356
BspTNI	GGTCTC	2 cut(s)	240, 654
BsrBI	CCGCTC	2 cut(s)	416, 475
BsrDI	GCAATG	2 cut(s)	1673, 1865
BsrGI	TGTACA	4 cut(s)	408, 1193, 2298, 2345
BsrI	ACTGG	4 cut(s)	886, 1082, 1420, 2226
BssECI	CCNNGG	7 cut(s)	105, 320, 1261, 1503, 1609, 1744, 2038
BssNI	GRCGYC	1 cut(s)	1864
BssSI	CACGAG	5 cut(s)	232, 854, 1465, 1518, 1916
BssT1I	CCWWGG	3 cut(s)	105, 1261, 2038
Bst2BI	CACGAG	5 cut(s)	232, 854, 1465, 1518, 1916
Bst2UI	CCWGG	4 cut(s)	1348, 1505, 1610, 1640
Bst4CI	ACNGT	5 cut(s)	439, 735, 831, 2316, 2325
Bst6I	CTCTTC	1 cut(s)	1963
BstACI	GRCGYC	1 cut(s)	1864
BstAPI	GCANNNNNTGC	2 cut(s)	1379, 2057
BstAUI	TGTACA	4 cut(s)	408, 1193, 2298, 2345
BstBI	TTCGAA	1 cut(s)	356
BstC8I	GCNNGC	5 cut(s)	191, 897, 1092, 1327, 1680
BstDEI	CTNAG	9 cut(s)	152, 545, 605, 636, 1758, 1808, 1997, 2211, 2366
BstDSI	CCRYGG	3 cut(s)	1261, 1744, 2038
BstF5I	GGATG	6 cut(s)	286, 766, 1257, 1283, 1297, 1754
BstFNI	CGCG	2 cut(s)	144, 1216
BstH2I	RGCGCY	1 cut(s)	716
BstHHI	GCGC	1 cut(s)	715
BstMAI	GTCTC	7 cut(s)	221, 240, 306, 654, 1824, 2314, 2455
BstMWI	GCNNNNNNNGC	8 cut(s)	615, 819, 1335, 1379, 1679, 1809, 2057, 2215
BstNI	CCWGG	4 cut(s)	1348, 1505, 1610, 1640
BstNSI	RCATGY	2 cut(s)	598, 2098
BstSCI	CCNGG	5 cut(s)	756, 1346, 1503, 1608, 1638
BstSFI	CTRYAG	2 cut(s)	187, 1834
BstSLI	GKGCMC	3 cut(s)	1684, 1757, 2251
BstUI	CGCG	2 cut(s)	144, 1216
BstV1I	GCAGC	3 cut(s)	34, 719, 2044
BstV2I	GAAGAC	2 cut(s)	491, 1667
BstX2I	RGATCY	4 cut(s)	324, 1039, 1080, 2499
BstYI	RGATCY	4 cut(s)	324, 1039, 1080, 2499
Bsu15I	ATCGAT	1 cut(s)	872
Bsu36I	CCTNAGG	1 cut(s)	2211
BsuI	GTATCC	2 cut(s)	279, 2388
BsuRI	GGCC	2 cut(s)	1845, 2209
BsuTUI	ATCGAT	1 cut(s)	872
BtgI	CCRYGG	3 cut(s)	1261, 1744, 2038
BtsCI	GGATG	6 cut(s)	286, 766, 1257, 1283, 1297, 1754
BtsI	GCAGTG	2 cut(s)	1368, 1459
BtsIMutI	CAGTG	7 cut(s)	373, 666, 1298, 1368, 1413, 1459, 1755
BtuMI	TCGCGA	2 cut(s)	144, 1216
Cac8I	GCNNGC	5 cut(s)	191, 897, 1092, 1327, 1680
CaiI	CAGNNNCTG	3 cut(s)	219, 1415, 2057
CciI	TCATGA	1 cut(s)	562
CfoI	GCGC	1 cut(s)	715
Cfr13I	GGNCC	3 cut(s)	79, 1599, 2233
ClaI	ATCGAT	1 cut(s)	872
CseI	GACGC	1 cut(s)	1853
CsiI	ACCWGGT	1 cut(s)	1346
Csp6I	GTAC	6 cut(s)	409, 1194, 1351, 1481, 2299, 2346
CspCI	CAANNNNNGTGG	2 cut(s)	898, 933
CviQI	GTAC	6 cut(s)	409, 1194, 1351, 1481, 2299, 2346
DdeI	CTNAG	9 cut(s)	152, 545, 605, 636, 1758, 1808, 1997, 2211, 2366
DraI	TTTAAA	2 cut(s)	1113, 2329
EaeI	YGGCCR	1 cut(s)	1843
Eam1104I	CTCTTC	1 cut(s)	1963
EarI	CTCTTC	1 cut(s)	1963
EciI	GGCGGA	2 cut(s)	512, 1709
Eco130I	CCWWGG	3 cut(s)	105, 1261, 2038
Eco31I	GGTCTC	2 cut(s)	240, 654
Eco47I	GGWCC	3 cut(s)	79, 1599, 2233
Eco57I	CTGAAG	1 cut(s)	313
Eco81I	CCTNAGG	1 cut(s)	2211
Eco88I	CYCGRG	2 cut(s)	319, 2076
EcoRI	GAATTC	1 cut(s)	2018
EcoRII	CCWGG	4 cut(s)	1346, 1503, 1608, 1638
EcoT14I	CCWWGG	3 cut(s)	105, 1261, 2038
ErhI	CCWWGG	3 cut(s)	105, 1261, 2038
FalI	AAGNNNNNCTT	2 cut(s)	1092, 1124
FaqI	GGGAC	3 cut(s)	1136, 1612, 1940
FauI	CCCGC	1 cut(s)	1671
FbaI	TGATCA	1 cut(s)	1057
Fnu4HI	GCNGC	6 cut(s)	23, 708, 711, 1649, 2058, 2207
FokI	GGATG	6 cut(s)	293, 773, 1244, 1270, 1284, 1761
Fsp4HI	GCNGC	6 cut(s)	23, 708, 711, 1649, 2058, 2207
FspBI	CTAG	7 cut(s)	426, 809, 896, 1335, 1775, 2333, 2510
GlaI	GCGC	1 cut(s)	714
GluI	GCNGC	6 cut(s)	23, 708, 711, 1649, 2058, 2207
GsuI	CTGGAG	3 cut(s)	1031, 1437, 2139
HaeII	RGCGCY	1 cut(s)	716
HaeIII	GGCC	2 cut(s)	1845, 2209
HapII	CCGG	6 cut(s)	77, 529, 541, 757, 1084, 2181
HgaI	GACGC	1 cut(s)	1853
HhaI	GCGC	1 cut(s)	715
Hin1I	GRCGYC	1 cut(s)	1864
Hin6I	GCGC	1 cut(s)	713
HinP1I	GCGC	1 cut(s)	713
HincII	GTYRAC	1 cut(s)	2197
HindII	GTYRAC	1 cut(s)	2197
HindIII	AAGCTT	3 cut(s)	946, 1106, 1474
HpaII	CCGG	6 cut(s)	77, 529, 541, 757, 1084, 2181
HphI	GGTGA	5 cut(s)	215, 509, 518, 746, 2091
Hpy166II	GTNNAC	8 cut(s)	6, 409, 1196, 1353, 1755, 2197, 2299, 2348
Hpy188I	TCNGA	6 cut(s)	125, 308, 654, 982, 1062, 2307
Hpy8I	GTNNAC	8 cut(s)	6, 409, 1196, 1353, 1755, 2197, 2299, 2348
Hpy99I	CGWCG	2 cut(s)	65, 144
HpyAV	CCTTC	7 cut(s)	530, 678, 1023, 1094, 1707, 1814, 2302
HpyCH4III	ACNGT	5 cut(s)	439, 735, 831, 2316, 2325
HpyF10VI	GCNNNNNNNGC	8 cut(s)	615, 819, 1335, 1379, 1679, 1809, 2057, 2215
HpyF3I	CTNAG	9 cut(s)	152, 545, 605, 636, 1758, 1808, 1997, 2211, 2366
Hsp92I	GRCGYC	1 cut(s)	1864
HspAI	GCGC	1 cut(s)	713
Kpn2I	TCCGGA	1 cut(s)	1083
Ksp22I	TGATCA	1 cut(s)	1057
LmnI	GCTCC	5 cut(s)	340, 676, 1722, 1746, 2357
Lsp1109I	GCAGC	3 cut(s)	34, 719, 2044
LweI	GCATC	6 cut(s)	771, 864, 1186, 1382, 1391, 2053
MabI	ACCWGGT	1 cut(s)	1346
MaeI	CTAG	7 cut(s)	426, 809, 896, 1335, 1775, 2333, 2510
MaeIII	GTNAC	6 cut(s)	180, 221, 524, 1405, 2090, 2097
MbiI	CCGCTC	2 cut(s)	416, 475
MboII	GAAGA	6 cut(s)	319, 496, 716, 1672, 1950, 2465
MfeI	CAATTG	1 cut(s)	670
MflI	RGATCY	4 cut(s)	324, 1039, 1080, 2499
MhlI	GDGCHC	4 cut(s)	855, 1684, 1757, 2251
MlsI	TGGCCA	1 cut(s)	1845
MluNI	TGGCCA	1 cut(s)	1845
MlyI	GAGTC	3 cut(s)	163, 188, 1989
MmeI	TCCRAC	3 cut(s)	286, 677, 717
Mox20I	TGGCCA	1 cut(s)	1845
MroI	TCCGGA	1 cut(s)	1083
MroXI	GAANNNNTTC	1 cut(s)	362
MscI	TGGCCA	1 cut(s)	1845
MseI	TTAA	5 cut(s)	798, 1112, 1701, 2328, 2493
MslI	CAYNNNNRTG	5 cut(s)	17, 162, 759, 798, 2104
Msp20I	TGGCCA	1 cut(s)	1845
MspA1I	CMGCKG	2 cut(s)	25, 710
MspI	CCGG	6 cut(s)	77, 529, 541, 757, 1084, 2181
MspR9I	CCNGG	5 cut(s)	758, 1348, 1505, 1610, 1640
MssI	GTTTAAAC	1 cut(s)	2329
MunI	CAATTG	1 cut(s)	670
Mva1269I	GAATGC	1 cut(s)	152
MvaI	CCWGG	4 cut(s)	1348, 1505, 1610, 1640
MvnI	CGCG	2 cut(s)	144, 1216
MwoI	GCNNNNNNNGC	8 cut(s)	615, 819, 1335, 1379, 1679, 1809, 2057, 2215
NciI	CCSGG	1 cut(s)	758
NcoI	CCATGG	2 cut(s)	1261, 2038
NheI	GCTAGC	1 cut(s)	895
NlaIV	GGNNCC	5 cut(s)	1082, 1600, 1718, 2234, 2501
NmeAIII	GCCGAG	2 cut(s)	784, 1299
NmuCI	GTSAC	5 cut(s)	180, 221, 524, 1405, 2097
NruI	TCGCGA	2 cut(s)	144, 1216
NspI	RCATGY	2 cut(s)	598, 2098
NspV	TTCGAA	1 cut(s)	356
PaeR7I	CTCGAG	1 cut(s)	2076
PagI	TCATGA	1 cut(s)	562
PciI	ACATGT	1 cut(s)	2094
PctI	GAATGC	1 cut(s)	152
PdmI	GAANNNNTTC	1 cut(s)	362
PfeI	GAWTC	9 cut(s)	130, 532, 788, 1132, 1568, 1686, 1903, 2114, 2474
PflFI	GACNNNGTC	1 cut(s)	302
PkrI	GCNGC	6 cut(s)	24, 709, 712, 1650, 2059, 2208
PleI	GAGTC	3 cut(s)	162, 187, 1989
PmeI	GTTTAAAC	1 cut(s)	2329
PpsI	GAGTC	3 cut(s)	162, 187, 1989
PscI	ACATGT	1 cut(s)	2094
PsiI	TTATAA	1 cut(s)	2177
Psp6I	CCWGG	4 cut(s)	1346, 1503, 1608, 1638
PspGI	CCWGG	4 cut(s)	1346, 1503, 1608, 1638
PspN4I	GGNNCC	5 cut(s)	1082, 1600, 1718, 2234, 2501
PspPI	GGNCC	3 cut(s)	79, 1599, 2233
PspXI	VCTCGAGB	1 cut(s)	2076
PstI	CTGCAG	1 cut(s)	191
PstNI	CAGNNNCTG	3 cut(s)	219, 1415, 2057
PsuI	RGATCY	4 cut(s)	324, 1039, 1080, 2499
PsyI	GACNNNGTC	1 cut(s)	302
PvuII	CAGCTG	1 cut(s)	25
RruI	TCGCGA	2 cut(s)	144, 1216
RsaI	GTAC	6 cut(s)	410, 1195, 1352, 1482, 2300, 2347
RsaNI	GTAC	6 cut(s)	409, 1194, 1351, 1481, 2299, 2346
RseI	CAYNNNNRTG	5 cut(s)	17, 162, 759, 798, 2104
SaqAI	TTAA	5 cut(s)	798, 1112, 1701, 2328, 2493
SatI	GCNGC	6 cut(s)	23, 708, 711, 1649, 2058, 2207
Sau96I	GGNCC	3 cut(s)	79, 1599, 2233
SchI	GAGTC	3 cut(s)	163, 188, 1989
ScrFI	CCNGG	5 cut(s)	758, 1348, 1505, 1610, 1640
SduI	GDGCHC	4 cut(s)	855, 1684, 1757, 2251
SexAI	ACCWGGT	1 cut(s)	1346
SfaNI	GCATC	6 cut(s)	771, 864, 1186, 1382, 1391, 2053
SfcI	CTRYAG	2 cut(s)	187, 1834
Sfr274I	CTCGAG	1 cut(s)	2076
SfuI	TTCGAA	1 cut(s)	356
SinI	GGWCC	3 cut(s)	79, 1599, 2233
SlaI	CTCGAG	1 cut(s)	2076
SmiMI	CAYNNNNRTG	5 cut(s)	17, 162, 759, 798, 2104
SmlI	CTYRAG	3 cut(s)	293, 2076, 2168
SmoI	CTYRAG	3 cut(s)	293, 2076, 2168
SspMI	CTAG	7 cut(s)	426, 809, 896, 1335, 1775, 2333, 2510
StyD4I	CCNGG	5 cut(s)	756, 1346, 1503, 1608, 1638
StyI	CCWWGG	3 cut(s)	105, 1261, 2038
TaaI	ACNGT	5 cut(s)	439, 735, 831, 2316, 2325
TaqI	TCGA	7 cut(s)	69, 356, 462, 480, 872, 1974, 2077
TaqII	GACCGA	1 cut(s)	2386
TatI	WGTACW	4 cut(s)	408, 1193, 2298, 2345
TauI	GCSGC	3 cut(s)	713, 1651, 2209
TfiI	GAWTC	9 cut(s)	130, 532, 788, 1132, 1568, 1686, 1903, 2114, 2474
Tru1I	TTAA	5 cut(s)	798, 1112, 1701, 2328, 2493
Tru9I	TTAA	5 cut(s)	798, 1112, 1701, 2328, 2493
TscAI	CASTG	7 cut(s)	373, 666, 1298, 1375, 1420, 1459, 1762
TseFI	GTSAC	5 cut(s)	180, 221, 524, 1405, 2097
TseI	GCWGC	3 cut(s)	22, 707, 2057
Tsp45I	GTSAC	5 cut(s)	180, 221, 524, 1405, 2097
TspDTI	ATGAA	9 cut(s)	31, 551, 579, 858, 1113, 1241, 1673, 1710, 2000
TspGWI	ACGGA	6 cut(s)	353, 1733, 2044, 2225, 2266, 2438
TspRI	CASTG	7 cut(s)	373, 666, 1298, 1375, 1420, 1459, 1762
Tth111I	GACNNNGTC	1 cut(s)	302
VneI	GTGCAC	1 cut(s)	1753
VpaK11BI	GGWCC	3 cut(s)	79, 1599, 2233
XapI	RAATTY	2 cut(s)	2018, 2240
XceI	RCATGY	2 cut(s)	598, 2098
XcmI	CCANNNNNNNNNTGG	2 cut(s)	257, 1227
XhoI	CTCGAG	1 cut(s)	2076
XmnI	GAANNNNTTC	1 cut(s)	362
XspI	CTAG	7 cut(s)	426, 809, 896, 1335, 1775, 2333, 2510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.