Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0011314

Physical Location & Seq

Genomic Coordinates Forward (+)

19775458 .. 19777692

Gene Structure 2235 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g15850.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1353 bp

ATGGAGAAGGAAGAGAACAACTACTCGGCTGCTTTCCTGCCACCAAGCTTTAACGGTCTCGATTACACCTTAGACCACCATCATGATCAAGATCAGCAGCAGCTGATGAACTCATCCCATGTCGACCAGACTTGTTCGAGTCACGAAAACAACAACAATGGAATGGTCAATTACTTGCTGAATGATCATCACCATGATCGGCTTCAACTTCAACATCAACATCAACAACCACAGCAGACGCAACATCCTCCTCCGGCAGGATTTGGTGGTGCTTCAAGTACTTCGTTTGATAAGCTGAGCTTTGCCGACGTGATGCAGTTTGCGGATTTTGGACCCAAACTAGCCTTAAACCAAACAAAGATTTCAGAAGACGAATCCGGGATTGACCCGGTTTACTTCCTTAAGTTTCCTGTTTTGAACGACAATAAGTTAGACGACGATGAGGATCAGTCTCTGATGGTTCCGCAGCAGGATGGACCAGACAGTCGTCAGGAAGAGAGATTTACAAAGTCCGGTGAAGAAGAGGAAACTCGAGGGGTTTCGGGTAGTACTAATAATTCGGTGCAGCTCCGGTTTCTTGGAGATGATGTTCAAAACCCTAGAGGAGGACTACTGGAGCCGCCTAAGAACAAGAGGAAAAGGCCAAGAAGTACTAAGACGAGTCAGGAAGTTGAAAGCCAGCGGATGACTCATATCGCCGTTGAGAGGAACCGAAGGAAGCAAATGAATGAGCATCTTCGAGTTCTCAGGTCACTCATGCCCGGTTCGTATGTACAAAGGGGGGATCAAGCTTCTATAATTGGAGGAGCTATAGACTTTGTTAGAGAGTTGGAGCAGCTCCTGCAGTGCTTGGAATCACAGAAGCGGAGAAGGCTCTTAGGCGAAGCGCCTCCTCCAAGACAAGTAGGAGCTGATTCCGCTGCGGCGGCTAATATGGCGGTGCTGCCTTCACAACCAGATCATCAAGGGGGTGGTGGCGTGCCATTCTTTCCGGTGCCTGATTTGCCTCTGAATCCAAATGATCCAAACAAGTTCGTGGACTTTGAAACGGGGCTCCGAGAAGAAACTGCCGAGAGCAAGTCGTGCTTCGCAGATGTTGAAGTGAAGGTTTTAGGGGGTGATGCCATGATCAAGATTCTTTCTCAGAGAAGACCTGGACAGCTCATTAAGGCCATTGCTGCCCTTGAAGATTTGCAGCTGACGATTCTTCACACTAACATCACCACAATCGAACAAACTGTTCTATATTGCTTTAAGGTTGCAAGTGAATCGAGGTTCACGGCTGAAGATATTGCAAGTTCGGTTCAACAGATATTCAGCTTTATCCATGCAAACACCGCCAGCGTCATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010016 GO:0010052 GO:0010103 GO:0010374 GO:0010377 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0019219 GO:0019222 GO:0030154 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045165 GO:0045595 GO:0045597 GO:0045893 GO:0045935 GO:0048367 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051302 GO:0051782 GO:0060255 GO:0061085 GO:0061086 GO:0065007 GO:0080090 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0140110 GO:1902275 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:2000112 GO:2001141 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

451

Amino Acids

50.26

Weight (kDa)

5.29

Isoelectric Point (pI)

57.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	232 - 283	1.9e-11	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	370 - 444	1.8e-07	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011314)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24140 AT3G24140
fragaria_vesca	FvH4_1g23290 FvH4_4g15850 FvH4_4g15850
malus_domestica	MD04G1006400.v1.1
prunus_persica	Prupe.1G006600_v2.0.a1 Prupe.1G006600_v2.0.a1 Prupe.1G006600_v2.0.a1
pyrus_communis	pycom04g00580
rosa_chinensis	RchiOBHm_Chr4g0415421
rosa_laevigata	RLG00000008071
rosa_multiflora	Rmu_sc0002402.1_g000006
rosa_roxburghii	Rroxscaffold_5G00359180
rosa_rugosa	Rorug04G0134300
rosa_samantha	Rh4AG194600 Rh4BG193300 Rh4CG205600 Rh4DG192300
rosa_wichuraiana	Rw4G016570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	483
AccB1I	GGYRCC	1 cut(s)	994
AccI	GTMKAC	1 cut(s)	123
AclWI	GGATC	3 cut(s)	453, 792, 1016
AcuI	CTGAAG	1 cut(s)	1305
AfaI	GTAC	4 cut(s)	280, 550, 652, 774
AfiI	CCNNNNNNNGG	3 cut(s)	257, 605, 705
AflII	CTTAAG	1 cut(s)	401
AjiI	CACGTC	1 cut(s)	310
AjnI	CCWGG	1 cut(s)	1153
AloI	GAACNNNNNNTCC	2 cut(s)	573, 605
Alw26I	GTCTC	2 cut(s)	62, 456
AlwI	GGATC	3 cut(s)	453, 792, 1016
AlwNI	CAGNNNCTG	3 cut(s)	103, 454, 841
Ama87I	CYCGRG	1 cut(s)	531
AoxI	GGCC	2 cut(s)	641, 1170
AspLEI	GCGC	1 cut(s)	889
AspS9I	GGNCC	2 cut(s)	332, 476
AsuC2I	CCSGG	3 cut(s)	379, 389, 762
AsuHPI	GGTGA	4 cut(s)	182, 527, 1130, 1213
AvaI	CYCGRG	1 cut(s)	531
AvaII	GGWCC	2 cut(s)	332, 476
BanI	GGYRCC	1 cut(s)	994
BanII	GRGCYC	1 cut(s)	1056
BbsI	GAAGAC	2 cut(s)	375, 1156
BccI	CCATC	3 cut(s)	87, 451, 467
BceAI	ACGGC	2 cut(s)	683, 1296
BcgI	CGANNNNNNTGC	2 cut(s)	1251, 1285
BciT130I	CCWGG	1 cut(s)	1155
BclI	TGATCA	3 cut(s)	85, 184, 1128
BcnI	CCSGG	3 cut(s)	379, 389, 762
BcoDI	GTCTC	2 cut(s)	62, 456
BfaI	CTAG	2 cut(s)	341, 600
BfmI	CTRYAG	2 cut(s)	810, 842
BfoI	RGCGCY	1 cut(s)	890
BfrI	CTTAAG	1 cut(s)	401
BlpI	GCTNAGC	1 cut(s)	296
BmcAI	AGTACT	3 cut(s)	280, 550, 652
Bme1390I	CCNGG	4 cut(s)	379, 389, 762, 1155
Bme18I	GGWCC	2 cut(s)	332, 476
BmeT110I	CYCGRG	1 cut(s)	531
BmgBI	CACGTC	1 cut(s)	310
BmgT120I	GGNCC	2 cut(s)	332, 476
BmiI	GGNNCC	6 cut(s)	334, 462, 618, 710, 996, 1055
BmrFI	CCNGG	4 cut(s)	379, 389, 762, 1155
BmsI	GCATC	3 cut(s)	303, 742, 1111
BoxI	GACNNNNGTC	1 cut(s)	486
BpiI	GAAGAC	2 cut(s)	375, 1156
BpmI	CTGGAG	1 cut(s)	635
Bpu1102I	GCTNAGC	1 cut(s)	296
BpuMI	CCSGG	3 cut(s)	379, 389, 762
BsaBI	GATNNNNATC	2 cut(s)	90, 444
BsaI	GGTCTC	1 cut(s)	62
BsaWI	WCCGGW	3 cut(s)	512, 570, 991
BsaXI	ACNNNNNCTCC	2 cut(s)	573, 603
Bsc4I	CCNNNNNNNGG	3 cut(s)	257, 605, 705
Bse1I	ACTGG	1 cut(s)	618
Bse3DI	GCAATG	1 cut(s)	1173
Bse8I	GATNNNNATC	2 cut(s)	90, 444
BseBI	CCWGG	1 cut(s)	1155
BseGI	GGATG	4 cut(s)	113, 244, 478, 690
BseJI	GATNNNNATC	2 cut(s)	90, 444
BseLI	CCNNNNNNNGG	3 cut(s)	257, 605, 705
BseMI	GCAATG	1 cut(s)	1173
BseMII	CTCAG	3 cut(s)	287, 760, 1157
BseNI	ACTGG	1 cut(s)	618
BseRI	GAGGAG	4 cut(s)	240, 618, 819, 882
BsgI	GTGCAG	1 cut(s)	584
BshFI	GGCC	2 cut(s)	643, 1172
BshNI	GGYRCC	1 cut(s)	994
BsiHKCI	CYCGRG	1 cut(s)	531
BsiSI	CCGG	7 cut(s)	254, 378, 389, 513, 571, 762, 992
BslI	CCNNNNNNNGG	3 cut(s)	257, 605, 705
BsmAI	GTCTC	2 cut(s)	62, 456
BsnI	GGCC	2 cut(s)	643, 1172
Bso31I	GGTCTC	1 cut(s)	62
BsoBI	CYCGRG	1 cut(s)	531
Bsp1286I	GDGCHC	1 cut(s)	1056
Bsp1407I	TGTACA	1 cut(s)	772
Bsp143I	GATC	9 cut(s)	85, 91, 184, 196, 445, 784, 958, 1021, 1128
Bsp1720I	GCTNAGC	1 cut(s)	296
BspANI	GGCC	2 cut(s)	643, 1172
BspCNI	CTCAG	3 cut(s)	288, 759, 1156
BspHI	TCATGA	1 cut(s)	82
BspLI	GGNNCC	6 cut(s)	334, 462, 618, 710, 996, 1055
BspMAI	CTGCAG	1 cut(s)	846
BspPI	GGATC	3 cut(s)	453, 792, 1016
BspT107I	GGYRCC	1 cut(s)	994
BspTI	CTTAAG	1 cut(s)	401
BspTNI	GGTCTC	1 cut(s)	62
BsrDI	GCAATG	1 cut(s)	1173
BsrGI	TGTACA	1 cut(s)	772
BsrI	ACTGG	1 cut(s)	618
BssMI	GATC	9 cut(s)	85, 91, 184, 196, 445, 784, 958, 1021, 1128
Bst2UI	CCWGG	1 cut(s)	1155
Bst4CI	ACNGT	3 cut(s)	56, 485, 1240
Bst6I	CTCTTC	3 cut(s)	6, 489, 516
BstAFI	CTTAAG	1 cut(s)	401
BstAPI	GCANNNNNTGC	2 cut(s)	841, 1083
BstAUI	TGTACA	1 cut(s)	772
BstC8I	GCNNGC	3 cut(s)	680, 980, 1342
BstDEI	CTNAG	7 cut(s)	70, 296, 624, 654, 746, 877, 1143
BstENI	CCTNNNNNAGG	2 cut(s)	255, 603
BstF5I	GGATG	4 cut(s)	113, 244, 478, 690
BstH2I	RGCGCY	1 cut(s)	890
BstHHI	GCGC	1 cut(s)	889
BstKTI	GATC	9 cut(s)	88, 94, 187, 199, 448, 787, 961, 1024, 1131
BstMAI	GTCTC	2 cut(s)	62, 456
BstMBI	GATC	9 cut(s)	85, 91, 184, 196, 445, 784, 958, 1021, 1128
BstMWI	GCNNNNNNNGC	9 cut(s)	841, 871, 917, 926, 935, 1003, 1083, 1178, 1337
BstNI	CCWGG	1 cut(s)	1155
BstPAI	GACNNNNGTC	1 cut(s)	486
BstSCI	CCNGG	4 cut(s)	377, 387, 760, 1153
BstSFI	CTRYAG	2 cut(s)	810, 842
BstV2I	GAAGAC	2 cut(s)	375, 1156
BsuRI	GGCC	2 cut(s)	643, 1172
BtrI	CACGTC	1 cut(s)	310
BtsCI	GGATG	4 cut(s)	113, 244, 478, 690
BtsI	GCAGTG	1 cut(s)	851
BtsIMutI	CAGTG	1 cut(s)	851
Cac8I	GCNNGC	3 cut(s)	680, 980, 1342
CaiI	CAGNNNCTG	3 cut(s)	103, 454, 841
CciI	TCATGA	1 cut(s)	82
CfoI	GCGC	1 cut(s)	889
Cfr13I	GGNCC	2 cut(s)	332, 476
CseI	GACGC	2 cut(s)	247, 1333
Csp6I	GTAC	4 cut(s)	279, 549, 651, 773
CviAII	CATG	7 cut(s)	83, 119, 194, 757, 1126, 1328, 1348
CviQI	GTAC	4 cut(s)	279, 549, 651, 773
DdeI	CTNAG	7 cut(s)	70, 296, 624, 654, 746, 877, 1143
DpnI	GATC	9 cut(s)	87, 93, 186, 198, 447, 786, 960, 1023, 1130
DpnII	GATC	9 cut(s)	85, 91, 184, 196, 445, 784, 958, 1021, 1128
DrdI	GACNNNNNNGTC	1 cut(s)	483
DseDI	GACNNNNNNGTC	1 cut(s)	483
Eam1104I	CTCTTC	3 cut(s)	6, 489, 516
EarI	CTCTTC	3 cut(s)	6, 489, 516
Eco24I	GRGCYC	1 cut(s)	1056
Eco31I	GGTCTC	1 cut(s)	62
Eco47I	GGWCC	2 cut(s)	332, 476
Eco57I	CTGAAG	1 cut(s)	1305
Eco88I	CYCGRG	1 cut(s)	531
EcoNI	CCTNNNNNAGG	2 cut(s)	255, 603
EcoRII	CCWGG	1 cut(s)	1153
EcoT38I	GRGCYC	1 cut(s)	1056
FaeI	CATG	7 cut(s)	86, 122, 197, 760, 1129, 1331, 1351
FalI	AAGNNNNNCTT	4 cut(s)	284, 316, 1070, 1102
FatI	CATG	7 cut(s)	82, 118, 193, 756, 1125, 1327, 1347
FbaI	TGATCA	3 cut(s)	85, 184, 1128
FblI	GTMKAC	1 cut(s)	123
FokI	GGATG	4 cut(s)	100, 231, 485, 697
FriOI	GRGCYC	1 cut(s)	1056
FspBI	CTAG	2 cut(s)	341, 600
GlaI	GCGC	1 cut(s)	888
GsuI	CTGGAG	1 cut(s)	635
HaeII	RGCGCY	1 cut(s)	890
HaeIII	GGCC	2 cut(s)	643, 1172
HapII	CCGG	7 cut(s)	254, 378, 389, 513, 571, 762, 992
HgaI	GACGC	2 cut(s)	247, 1333
HhaI	GCGC	1 cut(s)	889
Hin1II	CATG	7 cut(s)	86, 122, 197, 760, 1129, 1331, 1351
Hin6I	GCGC	1 cut(s)	887
HinP1I	GCGC	1 cut(s)	887
HincII	GTYRAC	1 cut(s)	124
HindII	GTYRAC	1 cut(s)	124
HindIII	AAGCTT	2 cut(s)	46, 789
HpaII	CCGG	7 cut(s)	254, 378, 389, 513, 571, 762, 992
HphI	GGTGA	4 cut(s)	182, 527, 1130, 1213
Hpy166II	GTNNAC	4 cut(s)	124, 394, 1039, 1278
Hpy188I	TCNGA	5 cut(s)	367, 456, 1011, 1058, 1146
Hpy188III	TCNNGA	7 cut(s)	59, 83, 89, 143, 491, 665, 1132
Hpy8I	GTNNAC	4 cut(s)	124, 394, 1039, 1278
Hpy99I	CGWCG	2 cut(s)	311, 440
HpyAV	CCTTC	4 cut(s)	708, 864, 957, 1099
HpyCH4III	ACNGT	3 cut(s)	56, 485, 1240
HpyCH4IV	ACGT	1 cut(s)	309
HpyCH4V	TGCA	7 cut(s)	316, 565, 844, 1195, 1262, 1295, 1331
HpyF10VI	GCNNNNNNNGC	9 cut(s)	841, 871, 917, 926, 935, 1003, 1083, 1178, 1337
HpyF3I	CTNAG	7 cut(s)	70, 296, 624, 654, 746, 877, 1143
HpySE526I	ACGT	1 cut(s)	309
Hsp92II	CATG	7 cut(s)	86, 122, 197, 760, 1129, 1331, 1351
HspAI	GCGC	1 cut(s)	887
Ksp22I	TGATCA	3 cut(s)	85, 184, 1128
Kzo9I	GATC	9 cut(s)	85, 91, 184, 196, 445, 784, 958, 1021, 1128
LmnI	GCTCC	7 cut(s)	573, 616, 806, 832, 843, 908, 1059
LweI	GCATC	3 cut(s)	303, 742, 1111
MaeI	CTAG	2 cut(s)	341, 600
MaeII	ACGT	1 cut(s)	309
MaeIII	GTNAC	2 cut(s)	140, 750
MalI	GATC	9 cut(s)	87, 93, 186, 198, 447, 786, 960, 1023, 1130
MboI	GATC	9 cut(s)	85, 91, 184, 196, 445, 784, 958, 1021, 1128
MhlI	GDGCHC	1 cut(s)	1056
MluCI	AATT	3 cut(s)	169, 556, 798
MlyI	GAGTC	3 cut(s)	148, 670, 682
MmeI	TCCRAC	1 cut(s)	810
MseI	TTAA	5 cut(s)	51, 347, 402, 1167, 1254
MslI	CAYNNNNRTG	2 cut(s)	81, 192
MspA1I	CMGCKG	4 cut(s)	103, 682, 920, 1198
MspCI	CTTAAG	1 cut(s)	401
MspI	CCGG	7 cut(s)	254, 378, 389, 513, 571, 762, 992
MspR9I	CCNGG	4 cut(s)	379, 389, 762, 1155
MvaI	CCWGG	1 cut(s)	1155
MwoI	GCNNNNNNNGC	9 cut(s)	841, 871, 917, 926, 935, 1003, 1083, 1178, 1337
NciI	CCSGG	3 cut(s)	379, 389, 762
NdeII	GATC	9 cut(s)	85, 91, 184, 196, 445, 784, 958, 1021, 1128
NlaIII	CATG	7 cut(s)	86, 122, 197, 760, 1129, 1331, 1351
NlaIV	GGNNCC	6 cut(s)	334, 462, 618, 710, 996, 1055
NmeAIII	GCCGAG	2 cut(s)	5, 1096
NmuCI	GTSAC	2 cut(s)	140, 750
PaeR7I	CTCGAG	1 cut(s)	531
PagI	TCATGA	1 cut(s)	82
PfeI	GAWTC	7 cut(s)	374, 854, 914, 1012, 1135, 1204, 1268
PfoI	TCCNGGA	1 cut(s)	377
PleI	GAGTC	3 cut(s)	147, 669, 682
PpsI	GAGTC	3 cut(s)	147, 669, 682
PshAI	GACNNNNGTC	1 cut(s)	486
Psp6I	CCWGG	1 cut(s)	1153
PspGI	CCWGG	1 cut(s)	1153
PspN4I	GGNNCC	6 cut(s)	334, 462, 618, 710, 996, 1055
PspPI	GGNCC	2 cut(s)	332, 476
PspXI	VCTCGAGB	1 cut(s)	531
PstI	CTGCAG	1 cut(s)	846
PstNI	CAGNNNCTG	3 cut(s)	103, 454, 841
PvuII	CAGCTG	2 cut(s)	103, 1198
RsaI	GTAC	4 cut(s)	280, 550, 652, 774
RsaNI	GTAC	4 cut(s)	279, 549, 651, 773
RseI	CAYNNNNRTG	2 cut(s)	81, 192
SalI	GTCGAC	1 cut(s)	122
SaqAI	TTAA	5 cut(s)	51, 347, 402, 1167, 1254
Sau3AI	GATC	9 cut(s)	85, 91, 184, 196, 445, 784, 958, 1021, 1128
Sau96I	GGNCC	2 cut(s)	332, 476
ScaI	AGTACT	3 cut(s)	280, 550, 652
SchI	GAGTC	3 cut(s)	148, 670, 682
ScrFI	CCNGG	4 cut(s)	379, 389, 762, 1155
SduI	GDGCHC	1 cut(s)	1056
SfaNI	GCATC	3 cut(s)	303, 742, 1111
SfcI	CTRYAG	2 cut(s)	810, 842
Sfr274I	CTCGAG	1 cut(s)	531
SinI	GGWCC	2 cut(s)	332, 476
SlaI	CTCGAG	1 cut(s)	531
SmiMI	CAYNNNNRTG	2 cut(s)	81, 192
SmlI	CTYRAG	2 cut(s)	401, 531
SmoI	CTYRAG	2 cut(s)	401, 531
Sse9I	AATT	3 cut(s)	169, 556, 798
SspMI	CTAG	2 cut(s)	341, 600
StyD4I	CCNGG	4 cut(s)	377, 387, 760, 1153
TaaI	ACNGT	3 cut(s)	56, 485, 1240
TaiI	ACGT	1 cut(s)	312
TaqI	TCGA	7 cut(s)	60, 123, 137, 532, 739, 1230, 1271
TasI	AATT	3 cut(s)	169, 556, 798
TatI	WGTACW	4 cut(s)	278, 548, 650, 772
TauI	GCSGC	3 cut(s)	622, 926, 929
TfiI	GAWTC	7 cut(s)	374, 854, 914, 1012, 1135, 1204, 1268
Tru1I	TTAA	5 cut(s)	51, 347, 402, 1167, 1254
Tru9I	TTAA	5 cut(s)	51, 347, 402, 1167, 1254
TscAI	CASTG	1 cut(s)	851
TseFI	GTSAC	2 cut(s)	140, 750
Tsp45I	GTSAC	2 cut(s)	140, 750
TspDTI	ATGAA	2 cut(s)	122, 740
TspRI	CASTG	1 cut(s)	851
Vha464I	CTTAAG	1 cut(s)	401
VpaK11BI	GGWCC	2 cut(s)	332, 476
XagI	CCTNNNNNAGG	2 cut(s)	255, 603
XhoI	CTCGAG	1 cut(s)	531
XmiI	GTMKAC	1 cut(s)	123
XspI	CTAG	2 cut(s)	341, 600
ZrmI	AGTACT	3 cut(s)	280, 550, 652

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.