Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0011314

Physical Location & Seq

Genomic Coordinates Forward (+)

47795397 .. 47797721

Gene Structure 2325 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG194600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGAGAAGGAAGAGAACAACTACTCGGCTGCTTTCCTGCCAACTAATTTTAATGGTCTGGATTACACCTTAGACCACCATCATGATGAAGATCAGCAGCAGCAGCAGATGAACTCATCCCATGTCGACCTGACTTCTTCGAGTCACGAAAACAACAACAATGGAATGATCAATTACTTGATGAATGATCATCACCATGATCAGCTTCAACTTCAACATCAACAGCCACAACAGACGCAACATCCTCCTCCGGCAGGGTTTAGTGGTGCTTCAACTACTTCTTTTGATAAGCTGAGCTTCGCGGACGTGATGCAGTTTGCCGATTTTGGACCCAAACTAGCCTTAAACCAAACCAAGATCTCAGAAGAAGAATCCGGGATTGACCCGGTTTACTTCCTCAAGTTTCCGGTTTTGAACGACAAGTTGGACGACGATGACGATCAGTCTCTGATGGTTCCGCGGCAGGATGGACCGGACAGTCATCAGGAAGAGAGATTTACAAAGTCCGGGGAAGAAGAGGAAACTCGAGGGGTTTCGGGTAGTACTAATTCGGTGCAGCTCCGGTTTCTTGGAGATGATGTTCCAAACCCTAGAGGAGGACTACCGGAGCTGCCAAAGAACAAGAGGAAAAGACCAAGAAGTACTAAGACGAGTCAGGAAGTTGAGAGCCAGAGGATGACTCACATCGCCGTTGAGAGGAACCGAAGGAAGCAAATGAATGAGCATCTTCGAGTGCTCAGGTCCCTCATGCCCGGTTCATATGTACAAAGGGGGGATCAAGCTTCTATTATTGGAGGAGCCATAGACTTTGTTAGAGAATTGGAGCAGCTCCTGCAGTGCTTGGAATCACAGAAGCGGAGAAGGCTCTTAGGTGAAGCGCCTCCTCCGAGACAAGTGGGAGCTGACCCTACTGCGGCTGCTAATATGGCGGTGCTACCTTCACAGCCAGATCATCAAGGCGGTGGTGGTGTGCCGTTCTTTCCGGCTCCTAATCTGCCTCTTAATCCAAATGATCCAATCAAGTTTGTGGACTTTGAAACGGGGCTCCGCGAAGAAACTGCCGAGATCAAGTCGTGCTTCGCAGATGTTGAAGTGAAGGTTTTAGGGGGTGATGCCATGATCAAGATTCTTTCTCAGAGAAGACCGGGCCAGCTCATTAAGGCCATTGCTGCCCTTGAAGATTTGCAGCTTAAAATTCTTCACACTAACATCACCACCATCGAACAAACTGTTCTATATTGCTTTAAGGTATGTACTTCTATACATCTATGTATATGTGTGTACTTATATCCAGCTGATTACTTGTATATAATGAATGTACGTGATATATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010016 GO:0010052 GO:0010103 GO:0010374 GO:0010377 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010628 GO:0010639 GO:0019219 GO:0019222 GO:0030154 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045165 GO:0045595 GO:0045597 GO:0045893 GO:0045935 GO:0048367 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051302 GO:0051782 GO:0060255 GO:0061085 GO:0061086 GO:0065007 GO:0080090 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0140110 GO:1902275 GO:1902680 GO:1903506 GO:1903508 GO:1905268 GO:2000112 GO:2001141 GO:2001251

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

444

Amino Acids

49.96

Weight (kDa)

5.34

Isoelectric Point (pI)

53.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	224 - 275	1.9e-11	Helix-loop-helix DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011314)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24140 AT3G24140
fragaria_vesca	FvH4_1g23290 FvH4_4g15850 FvH4_4g15850
malus_domestica	MD04G1006400.v1.1
prunus_persica	Prupe.1G006600_v2.0.a1 Prupe.1G006600_v2.0.a1 Prupe.1G006600_v2.0.a1
pyrus_communis	pycom04g00580
rosa_chinensis	RchiOBHm_Chr4g0415421
rosa_laevigata	RLG00000008071
rosa_multiflora	Rmu_sc0002402.1_g000006
rosa_roxburghii	Rroxscaffold_5G00359180
rosa_rugosa	Rorug04G0134300
rosa_samantha	Rh4AG194600 Rh4BG193300 Rh4CG205600 Rh4DG192300
rosa_wichuraiana	Rw4G016570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	477
AccI	GTMKAC	1 cut(s)	126
AccII	CGCG	3 cut(s)	302, 460, 1050
AciI	CCGC	8 cut(s)	302, 458, 460, 856, 914, 929, 960, 1048
AclWI	GGATC	2 cut(s)	783, 1007
AcsI	RAATTY	1 cut(s)	1194
AfaI	GTAC	6 cut(s)	544, 643, 765, 1255, 1283, 1320
AfiI	CCNNNNNNNGG	4 cut(s)	254, 596, 696, 913
AgsI	TTSAA	7 cut(s)	209, 215, 273, 415, 1037, 1091, 1178
AhdI	GACNNNNNGTC	1 cut(s)	442
AjiI	CACGTC	1 cut(s)	307
AjuI	GAANNNNNNNTTGG	2 cut(s)	407, 439
AloI	GAACNNNNNNTCC	2 cut(s)	564, 596
Alw21I	GWGCWC	1 cut(s)	738
Alw26I	GTCTC	2 cut(s)	450, 883
AlwI	GGATC	2 cut(s)	783, 1007
AlwNI	CAGNNNCTG	2 cut(s)	448, 832
Ama87I	CYCGRG	1 cut(s)	525
AoxI	GGCC	2 cut(s)	1147, 1161
ApoI	RAATTY	1 cut(s)	1194
AspLEI	GCGC	1 cut(s)	880
AspS9I	GGNCC	4 cut(s)	329, 470, 741, 1147
AsuC2I	CCSGG	5 cut(s)	376, 386, 508, 753, 1146
AsuHPI	GGTGA	4 cut(s)	185, 884, 1121, 1204
AvaI	CYCGRG	1 cut(s)	525
AvaII	GGWCC	3 cut(s)	329, 470, 741
BanII	GRGCYC	1 cut(s)	1047
BbsI	GAAGAC	1 cut(s)	1147
Bbv12I	GWGCWC	1 cut(s)	738
BccI	CCATC	4 cut(s)	87, 445, 461, 1226
BceAI	ACGGC	2 cut(s)	674, 958
BcgI	CGANNNNNNTGC	2 cut(s)	1040, 1074
BclI	TGATCA	4 cut(s)	168, 187, 199, 1119
BcnI	CCSGG	5 cut(s)	376, 386, 508, 753, 1146
BcoDI	GTCTC	2 cut(s)	450, 883
BfaI	CTAG	2 cut(s)	338, 591
BfmI	CTRYAG	1 cut(s)	833
BfoI	RGCGCY	1 cut(s)	881
BglII	AGATCT	1 cut(s)	357
BlpI	GCTNAGC	1 cut(s)	293
BmcAI	AGTACT	2 cut(s)	544, 643
Bme1390I	CCNGG	5 cut(s)	376, 386, 508, 753, 1146
Bme18I	GGWCC	3 cut(s)	329, 470, 741
BmeRI	GACNNNNNGTC	1 cut(s)	442
BmeT110I	CYCGRG	1 cut(s)	525
BmgBI	CACGTC	1 cut(s)	307
BmgT120I	GGNCC	4 cut(s)	329, 470, 741, 1147
BmiI	GGNNCC	7 cut(s)	331, 456, 701, 743, 799, 987, 1046
BmrFI	CCNGG	5 cut(s)	376, 386, 508, 753, 1146
BmsI	GCATC	3 cut(s)	300, 733, 1102
BpiI	GAAGAC	1 cut(s)	1147
BplI	GAGNNNNNCTC	2 cut(s)	664, 696
Bpu10I	CCTNAGC	1 cut(s)	737
Bpu1102I	GCTNAGC	1 cut(s)	293
BpuEI	CTTGAG	1 cut(s)	383
BpuMI	CCSGG	5 cut(s)	376, 386, 508, 753, 1146
BsaAI	YACGTR	1 cut(s)	1322
BsaBI	GATNNNNATC	2 cut(s)	90, 438
BsaJI	CCNNGG	2 cut(s)	458, 507
BsaWI	WCCGGW	4 cut(s)	406, 472, 561, 604
BsaXI	ACNNNNNCTCC	2 cut(s)	564, 594
Bsc4I	CCNNNNNNNGG	4 cut(s)	254, 596, 696, 913
Bse3DI	GCAATG	1 cut(s)	1164
Bse8I	GATNNNNATC	2 cut(s)	90, 438
BseDI	CCNNGG	2 cut(s)	458, 507
BseGI	GGATG	4 cut(s)	116, 241, 472, 681
BseJI	GATNNNNATC	2 cut(s)	90, 438
BseLI	CCNNNNNNNGG	4 cut(s)	254, 596, 696, 913
BseMI	GCAATG	1 cut(s)	1164
BseMII	CTCAG	4 cut(s)	284, 375, 751, 1148
BseRI	GAGGAG	4 cut(s)	237, 609, 810, 873
BsgI	GTGCAG	1 cut(s)	575
Bsh1236I	CGCG	3 cut(s)	302, 460, 1050
BshFI	GGCC	2 cut(s)	1149, 1163
BsiHKAI	GWGCWC	1 cut(s)	738
BsiHKCI	CYCGRG	1 cut(s)	525
BslFI	GGGAC	1 cut(s)	727
BslI	CCNNNNNNNGG	4 cut(s)	254, 596, 696, 913
BsmAI	GTCTC	2 cut(s)	450, 883
BsmFI	GGGAC	1 cut(s)	727
BsnI	GGCC	2 cut(s)	1149, 1163
BsoBI	CYCGRG	1 cut(s)	525
Bsp1286I	GDGCHC	2 cut(s)	738, 1047
Bsp1407I	TGTACA	1 cut(s)	763
Bsp1720I	GCTNAGC	1 cut(s)	293
BspACI	CCGC	8 cut(s)	302, 458, 460, 856, 914, 929, 960, 1048
BspANI	GGCC	2 cut(s)	1149, 1163
BspCNI	CTCAG	4 cut(s)	285, 374, 750, 1147
BspFNI	CGCG	3 cut(s)	302, 460, 1050
BspHI	TCATGA	1 cut(s)	82
BspLI	GGNNCC	7 cut(s)	331, 456, 701, 743, 799, 987, 1046
BspMAI	CTGCAG	1 cut(s)	837
BspPI	GGATC	2 cut(s)	783, 1007
BsrDI	GCAATG	1 cut(s)	1164
BsrGI	TGTACA	1 cut(s)	763
BssECI	CCNNGG	2 cut(s)	458, 507
Bst4CI	ACNGT	2 cut(s)	479, 1231
Bst6I	CTCTTC	3 cut(s)	6, 483, 510
BstAPI	GCANNNNNTGC	1 cut(s)	832
BstAUI	TGTACA	1 cut(s)	763
BstBAI	YACGTR	1 cut(s)	1322
BstC8I	GCNNGC	1 cut(s)	1151
BstDEI	CTNAG	7 cut(s)	70, 293, 361, 645, 737, 868, 1134
BstDSI	CCRYGG	1 cut(s)	458
BstENI	CCTNNNNNAGG	2 cut(s)	252, 594
BstF5I	GGATG	4 cut(s)	116, 241, 472, 681
BstFNI	CGCG	3 cut(s)	302, 460, 1050
BstH2I	RGCGCY	1 cut(s)	881
BstHHI	GCGC	1 cut(s)	880
BstMAI	GTCTC	2 cut(s)	450, 883
BstMWI	GCNNNNNNNGC	5 cut(s)	103, 832, 862, 926, 1169
BstSCI	CCNGG	5 cut(s)	374, 384, 506, 751, 1144
BstSFI	CTRYAG	1 cut(s)	833
BstUI	CGCG	3 cut(s)	302, 460, 1050
BstV2I	GAAGAC	1 cut(s)	1147
BstX2I	RGATCY	1 cut(s)	357
BstYI	RGATCY	1 cut(s)	357
BsuRI	GGCC	2 cut(s)	1149, 1163
BtgI	CCRYGG	1 cut(s)	458
BtgZI	GCGATG	1 cut(s)	670
BtrI	CACGTC	1 cut(s)	307
BtsCI	GGATG	4 cut(s)	116, 241, 472, 681
BtsI	GCAGTG	1 cut(s)	842
BtsIMutI	CAGTG	1 cut(s)	842
Cac8I	GCNNGC	1 cut(s)	1151
CaiI	CAGNNNCTG	2 cut(s)	448, 832
CciI	TCATGA	1 cut(s)	82
CfoI	GCGC	1 cut(s)	880
Cfr13I	GGNCC	4 cut(s)	329, 470, 741, 1147
Cfr42I	CCGCGG	1 cut(s)	461
CseI	GACGC	1 cut(s)	244
Csp6I	GTAC	6 cut(s)	543, 642, 764, 1254, 1282, 1319
CviAII	CATG	5 cut(s)	83, 122, 197, 748, 1117
CviQI	GTAC	6 cut(s)	543, 642, 764, 1254, 1282, 1319
DdeI	CTNAG	7 cut(s)	70, 293, 361, 645, 737, 868, 1134
DrdI	GACNNNNNNGTC	1 cut(s)	477
DriI	GACNNNNNGTC	1 cut(s)	442
DseDI	GACNNNNNNGTC	1 cut(s)	477
Eam1104I	CTCTTC	3 cut(s)	6, 483, 510
Eam1105I	GACNNNNNGTC	1 cut(s)	442
EarI	CTCTTC	3 cut(s)	6, 483, 510
Eco24I	GRGCYC	1 cut(s)	1047
Eco47I	GGWCC	3 cut(s)	329, 470, 741
Eco88I	CYCGRG	1 cut(s)	525
EcoNI	CCTNNNNNAGG	2 cut(s)	252, 594
EcoO109I	RGGNCCY	1 cut(s)	741
EcoT38I	GRGCYC	1 cut(s)	1047
FaeI	CATG	5 cut(s)	86, 125, 200, 751, 1120
FalI	AAGNNNNNCTT	4 cut(s)	281, 313, 1061, 1093
FaqI	GGGAC	1 cut(s)	727
FatI	CATG	5 cut(s)	82, 121, 196, 747, 1116
FauNDI	CATATG	1 cut(s)	760
FbaI	TGATCA	4 cut(s)	168, 187, 199, 1119
FblI	GTMKAC	1 cut(s)	126
FokI	GGATG	4 cut(s)	103, 228, 479, 688
FriOI	GRGCYC	1 cut(s)	1047
FspBI	CTAG	2 cut(s)	338, 591
GlaI	GCGC	1 cut(s)	879
HaeII	RGCGCY	1 cut(s)	881
HaeIII	GGCC	2 cut(s)	1149, 1163
HgaI	GACGC	1 cut(s)	244
HhaI	GCGC	1 cut(s)	880
Hin1II	CATG	5 cut(s)	86, 125, 200, 751, 1120
Hin6I	GCGC	1 cut(s)	878
HinP1I	GCGC	1 cut(s)	878
HincII	GTYRAC	1 cut(s)	127
HindII	GTYRAC	1 cut(s)	127
HindIII	AAGCTT	1 cut(s)	780
HinfI	GANTC	6 cut(s)	142, 371, 652, 679, 845, 1126
HphI	GGTGA	4 cut(s)	185, 884, 1121, 1204
Hpy166II	GTNNAC	4 cut(s)	127, 391, 1030, 1282
Hpy188I	TCNGA	4 cut(s)	364, 450, 888, 1137
Hpy188III	TCNNGA	6 cut(s)	59, 83, 146, 485, 656, 1123
Hpy8I	GTNNAC	4 cut(s)	127, 391, 1030, 1282
Hpy99I	CGWCG	1 cut(s)	434
HpyAV	CCTTC	4 cut(s)	699, 855, 948, 1090
HpyCH4III	ACNGT	2 cut(s)	479, 1231
HpyCH4IV	ACGT	2 cut(s)	306, 1321
HpyCH4V	TGCA	4 cut(s)	313, 556, 835, 1186
HpyF10VI	GCNNNNNNNGC	5 cut(s)	103, 832, 862, 926, 1169
HpyF3I	CTNAG	7 cut(s)	70, 293, 361, 645, 737, 868, 1134
HpySE526I	ACGT	2 cut(s)	306, 1321
Hsp92II	CATG	5 cut(s)	86, 125, 200, 751, 1120
HspAI	GCGC	1 cut(s)	878
Ksp22I	TGATCA	4 cut(s)	168, 187, 199, 1119
KspI	CCGCGG	1 cut(s)	461
LmnI	GCTCC	8 cut(s)	564, 607, 797, 823, 834, 899, 991, 1050
LweI	GCATC	3 cut(s)	300, 733, 1102
MaeI	CTAG	2 cut(s)	338, 591
MaeII	ACGT	2 cut(s)	306, 1321
MaeIII	GTNAC	1 cut(s)	143
MflI	RGATCY	1 cut(s)	357
MhlI	GDGCHC	2 cut(s)	738, 1047
MluCI	AATT	5 cut(s)	46, 172, 547, 818, 1194
MlyI	GAGTC	3 cut(s)	151, 661, 673
MmeI	TCCRAC	1 cut(s)	405
MseI	TTAA	6 cut(s)	51, 344, 1002, 1158, 1191, 1245
MslI	CAYNNNNRTG	3 cut(s)	81, 84, 195
MspA1I	CMGCKG	2 cut(s)	460, 1295
MspR9I	CCNGG	5 cut(s)	376, 386, 508, 753, 1146
MvnI	CGCG	3 cut(s)	302, 460, 1050
MwoI	GCNNNNNNNGC	5 cut(s)	103, 832, 862, 926, 1169
NciI	CCSGG	5 cut(s)	376, 386, 508, 753, 1146
NdeI	CATATG	1 cut(s)	760
NlaIII	CATG	5 cut(s)	86, 125, 200, 751, 1120
NlaIV	GGNNCC	7 cut(s)	331, 456, 701, 743, 799, 987, 1046
NmeAIII	GCCGAG	2 cut(s)	5, 1087
NmuCI	GTSAC	1 cut(s)	143
PaeR7I	CTCGAG	1 cut(s)	525
PagI	TCATGA	1 cut(s)	82
PcsI	WCGNNNNNNNCGW	1 cut(s)	435
PfeI	GAWTC	3 cut(s)	371, 845, 1126
PfoI	TCCNGGA	1 cut(s)	374
PleI	GAGTC	3 cut(s)	150, 660, 673
PpsI	GAGTC	3 cut(s)	150, 660, 673
Ppu21I	YACGTR	1 cut(s)	1322
PpuMI	RGGWCCY	1 cut(s)	741
Psp5II	RGGWCCY	1 cut(s)	741
PspN4I	GGNNCC	7 cut(s)	331, 456, 701, 743, 799, 987, 1046
PspPI	GGNCC	4 cut(s)	329, 470, 741, 1147
PspPPI	RGGWCCY	1 cut(s)	741
PspXI	VCTCGAGB	1 cut(s)	525
PstI	CTGCAG	1 cut(s)	837
PstNI	CAGNNNCTG	2 cut(s)	448, 832
PsuI	RGATCY	1 cut(s)	357
PvuII	CAGCTG	1 cut(s)	1295
RsaI	GTAC	6 cut(s)	544, 643, 765, 1255, 1283, 1320
RsaNI	GTAC	6 cut(s)	543, 642, 764, 1254, 1282, 1319
RseI	CAYNNNNRTG	3 cut(s)	81, 84, 195
SacII	CCGCGG	1 cut(s)	461
SalI	GTCGAC	1 cut(s)	125
SaqAI	TTAA	6 cut(s)	51, 344, 1002, 1158, 1191, 1245
Sau96I	GGNCC	4 cut(s)	329, 470, 741, 1147
ScaI	AGTACT	2 cut(s)	544, 643
SchI	GAGTC	3 cut(s)	151, 661, 673
ScrFI	CCNGG	5 cut(s)	376, 386, 508, 753, 1146
SduI	GDGCHC	2 cut(s)	738, 1047
SfaNI	GCATC	3 cut(s)	300, 733, 1102
SfcI	CTRYAG	1 cut(s)	833
Sfr274I	CTCGAG	1 cut(s)	525
Sfr303I	CCGCGG	1 cut(s)	461
SgrBI	CCGCGG	1 cut(s)	461
SinI	GGWCC	3 cut(s)	329, 470, 741
SlaI	CTCGAG	1 cut(s)	525
SmiMI	CAYNNNNRTG	3 cut(s)	81, 84, 195
SmlI	CTYRAG	2 cut(s)	398, 525
SmoI	CTYRAG	2 cut(s)	398, 525
Sse9I	AATT	5 cut(s)	46, 172, 547, 818, 1194
SsiI	CCGC	8 cut(s)	302, 458, 460, 856, 914, 929, 960, 1048
SspMI	CTAG	2 cut(s)	338, 591
StyD4I	CCNGG	5 cut(s)	374, 384, 506, 751, 1144
TaaI	ACNGT	2 cut(s)	479, 1231
TaiI	ACGT	2 cut(s)	309, 1324
TaqI	TCGA	5 cut(s)	126, 140, 526, 730, 1221
TasI	AATT	5 cut(s)	46, 172, 547, 818, 1194
TatI	WGTACW	5 cut(s)	542, 641, 763, 1253, 1281
TauI	GCSGC	2 cut(s)	463, 917
TfiI	GAWTC	3 cut(s)	371, 845, 1126
Tru1I	TTAA	6 cut(s)	51, 344, 1002, 1158, 1191, 1245
Tru9I	TTAA	6 cut(s)	51, 344, 1002, 1158, 1191, 1245
TscAI	CASTG	1 cut(s)	842
TseFI	GTSAC	1 cut(s)	143
Tsp45I	GTSAC	1 cut(s)	143
TspDTI	ATGAA	6 cut(s)	102, 125, 197, 731, 747, 1328
TspRI	CASTG	1 cut(s)	842
VpaK11BI	GGWCC	3 cut(s)	329, 470, 741
XagI	CCTNNNNNAGG	2 cut(s)	252, 594
XapI	RAATTY	1 cut(s)	1194
XhoI	CTCGAG	1 cut(s)	525
XmiI	GTMKAC	1 cut(s)	126
XspI	CTAG	2 cut(s)	338, 591
ZrmI	AGTACT	2 cut(s)	544, 643

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.