FvH4_4g16510

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014577
Physical Location & Seq
Forward (+)
20424142 .. 20430102
5961 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16510.t1

Sequence Viewer

Length: 591 bp
ATGGCAGCAGCACCACCTTCTCTGTTCCTGTTCTTCCTCCTCCTCACAACCCTCGTGTGTATCAACACTACCGGAGTCGAAGGAGCCACAACAACTTGGTGCGTGGCGAGGAGTGATGCTAGCAACCAGGCTCTCCAGACTGCCTTGGACTATGCATGTGCCTCCGGCGCCGACTGCGTTCCAATCCAGTCCGATGGGCTGTGTTACCTCCCTAACACTATCCAAGCTCACACCTCGTACGCTTTCAACAGTTACTACCAGCGCAAGGCTATGGCTCCTGGCTCCTGTGACTTCTCCGGCACCGCCACCGTTGCCCAGACCGATCCCAGTTATGGATCTTGTGTATACCCTTCTTCTGTAAGCACGGCCGGAGGCACAAGTACTACACCAAACACAACTCCGACCACAACTACAACCATTCCAAGTCCACCAACGACGACAACGACGAATCCGACCTTTGGCAATGGCGTGGTGGGTGGACTCACTCCAGTAACAACTCCGGCTGTCCCCGATGATTCAAAAGCAGCATCCCTTCAATTCATGCTTCCAACATTGATTTCTGTCTCCTTTATTTTTTACATGTTCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

197

Amino Acids

20.34

Weight (kDa)

4.22

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 32 - 103 4.1e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13600
fragaria_vesca FvH4_4g16510 FvH4_4g16510
malus_domestica MD04G1009500.v1.1
prunus_persica Prupe.1G008800_v2.0.a1
pyrus_communis pycom04g00730
rosa_chinensis RchiOBHm_Chr4g0415121
rosa_laevigata RLG00000008096
rosa_multiflora Rmu_sc0000635.1_g000003 Rmu_sc0003748.1_g000016
rosa_roxburghii Rroxscaffold_5G00358920
rosa_rugosa Rorug04G0132400
rosa_samantha Rh4AG192200 Rh4BG190300 Rh4CG203000 Rh4DG190100
rosa_wichuraiana Rw4G016350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 167, 299
AccI GTMKAC 1 cut(s) 345
AciI CCGC 1 cut(s) 303
AclWI GGATC 2 cut(s) 317, 343
AcoI YGGCCR 1 cut(s) 366
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 2 cut(s) 239, 382
AfiI CCNNNNNNNGG 3 cut(s) 265, 332, 458
AflIII ACRYGT 1 cut(s) 579
AgsI TTSAA 3 cut(s) 247, 519, 536
AjnI CCWGG 2 cut(s) 126, 277
AjuI GAANNNNNNNTTGG 2 cut(s) 541, 573
AluBI AGCT 1 cut(s) 227
AluI AGCT 1 cut(s) 227
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 2 cut(s) 317, 343
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 3 cut(s) 5, 8, 524
AspLEI GCGC 2 cut(s) 170, 264
AsuNHI GCTAGC 1 cut(s) 119
BanI GGYRCC 2 cut(s) 167, 299
BauI CACGAG 1 cut(s) 53
BbvI GCAGC 3 cut(s) 17, 20, 536
BccI CCATC 1 cut(s) 188
BceAI ACGGC 1 cut(s) 381
BciT130I CCWGG 2 cut(s) 128, 279
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 3 cut(s) 6, 9, 525
BlsI GCNGC 3 cut(s) 7, 10, 526
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 128, 279
BmiI GGNNCC 5 cut(s) 85, 169, 276, 283, 301
BmrFI CCNGG 2 cut(s) 128, 279
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 106, 536
BmtI GCTAGC 1 cut(s) 123
BmuI ACTGGG 1 cut(s) 321
BpmI CTGGAG 2 cut(s) 119, 471
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 144
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 332, 458
Bse1I ACTGG 3 cut(s) 187, 327, 488
Bse3DI GCAATG 1 cut(s) 469
BseBI CCWGG 2 cut(s) 128, 279
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 527
BseLI CCNNNNNNNGG 3 cut(s) 265, 332, 458
BseMI GCAATG 1 cut(s) 469
BseNI ACTGG 3 cut(s) 187, 327, 488
BseRI GAGGAG 3 cut(s) 29, 32, 124
BseX3I CGGCCG 1 cut(s) 366
BseXI GCAGC 3 cut(s) 17, 20, 536
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 368
BshNI GGYRCC 2 cut(s) 167, 299
BsiEI CGRYCG 1 cut(s) 369
BsiSI CCGG 5 cut(s) 72, 165, 297, 369, 500
BsiWI CGTACG 1 cut(s) 237
BslFI GGGAC 1 cut(s) 491
BslI CCNNNNNNNGG 3 cut(s) 265, 332, 458
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 1 cut(s) 491
BsnI GGCC 1 cut(s) 368
Bsp143I GATC 2 cut(s) 322, 335
BspACI CCGC 1 cut(s) 303
BspANI GGCC 1 cut(s) 368
BspLI GGNNCC 5 cut(s) 85, 169, 276, 283, 301
BspOI GCTAGC 1 cut(s) 123
BspPI GGATC 2 cut(s) 317, 343
BspT107I GGYRCC 2 cut(s) 167, 299
BsrDI GCAATG 1 cut(s) 469
BsrI ACTGG 3 cut(s) 187, 327, 488
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 2 cut(s) 322, 335
BssNAI GTATAC 1 cut(s) 346
BssNI GRCGYC 1 cut(s) 168
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 144
Bst1107I GTATAC 1 cut(s) 346
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 128, 279
Bst4CI ACNGT 2 cut(s) 251, 310
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 121
BstF5I GGATG 1 cut(s) 527
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 170, 264
BstKTI GATC 2 cut(s) 325, 338
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 2 cut(s) 322, 335
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 3 cut(s) 167, 174, 311
BstNI CCWGG 2 cut(s) 128, 279
BstNSI RCATGY 2 cut(s) 159, 583
BstSCI CCNGG 2 cut(s) 126, 277
BstV1I GCAGC 3 cut(s) 17, 20, 536
BstX2I RGATCY 1 cut(s) 335
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 1 cut(s) 335
BstZ17I GTATAC 1 cut(s) 346
BstZI CGGCCG 1 cut(s) 366
BsuRI GGCC 1 cut(s) 368
BtsCI GGATG 1 cut(s) 527
Cac8I GCNNGC 1 cut(s) 121
CfoI GCGC 2 cut(s) 170, 264
Csp6I GTAC 2 cut(s) 238, 381
CviAII CATG 3 cut(s) 156, 541, 580
CviJI RGCY 9 cut(s) 86, 131, 199, 227, 269, 275, 282, 368, 503
CviKI_1 RGCY 9 cut(s) 86, 131, 199, 227, 269, 275, 282, 368, 503
CviQI GTAC 2 cut(s) 238, 381
DinI GGCGCC 1 cut(s) 169
DpnI GATC 2 cut(s) 324, 337
DpnII GATC 2 cut(s) 322, 335
EaeI YGGCCR 1 cut(s) 366
EagI CGGCCG 1 cut(s) 366
EclXI CGGCCG 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 144
Eco52I CGGCCG 1 cut(s) 366
EcoRII CCWGG 2 cut(s) 126, 277
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 157
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 3 cut(s) 159, 544, 583
FaiI YATR 7 cut(s) 153, 157, 272, 333, 346, 542, 581
FaqI GGGAC 1 cut(s) 491
FatI CATG 3 cut(s) 155, 540, 579
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 3 cut(s) 6, 9, 525
FokI GGATG 1 cut(s) 514
Fsp4HI GCNGC 3 cut(s) 6, 9, 525
FspBI CTAG 1 cut(s) 120
GlaI GCGC 2 cut(s) 169, 263
GluI GCNGC 3 cut(s) 6, 9, 525
GsuI CTGGAG 2 cut(s) 119, 471
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 368
HapII CCGG 5 cut(s) 72, 165, 297, 369, 500
HhaI GCGC 2 cut(s) 170, 264
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 3 cut(s) 159, 544, 583
Hin6I GCGC 2 cut(s) 168, 262
HinP1I GCGC 2 cut(s) 168, 262
HinfI GANTC 4 cut(s) 75, 448, 480, 515
HpaII CCGG 5 cut(s) 72, 165, 297, 369, 500
Hpy166II GTNNAC 3 cut(s) 346, 428, 479
Hpy188I TCNGA 3 cut(s) 193, 402, 453
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 3 cut(s) 346, 428, 479
Hpy99I CGWCG 2 cut(s) 439, 448
HpyAV CCTTC 4 cut(s) 27, 74, 360, 542
HpyCH4III ACNGT 2 cut(s) 251, 310
HpyCH4V TGCA 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 174, 311
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 3 cut(s) 159, 544, 583
HspAI GCGC 2 cut(s) 168, 262
KasI GGCGCC 1 cut(s) 167
Kzo9I GATC 2 cut(s) 322, 335
LmnI GCTCC 3 cut(s) 83, 280, 287
Lsp1109I GCAGC 3 cut(s) 17, 20, 536
LweI GCATC 2 cut(s) 106, 536
MaeI CTAG 1 cut(s) 120
MaeIII GTNAC 4 cut(s) 203, 251, 287, 490
MalI GATC 2 cut(s) 324, 337
MboI GATC 2 cut(s) 322, 335
MboII GAAGA 2 cut(s) 25, 345
MflI RGATCY 1 cut(s) 335
MluCI AATT 1 cut(s) 536
Mly113I GGCGCC 1 cut(s) 168
MlyI GAGTC 2 cut(s) 84, 474
MmeI TCCRAC 3 cut(s) 425, 476, 572
MnlI CCTC 9 cut(s) 47, 50, 53, 62, 102, 172, 218, 244, 365
Mph1103I ATGCAT 1 cut(s) 157
MspI CCGG 5 cut(s) 72, 165, 297, 369, 500
MspR9I CCNGG 2 cut(s) 128, 279
MvaI CCWGG 2 cut(s) 128, 279
MwoI GCNNNNNNNGC 3 cut(s) 167, 174, 311
NarI GGCGCC 1 cut(s) 168
NdeII GATC 2 cut(s) 322, 335
NheI GCTAGC 1 cut(s) 119
NlaIII CATG 3 cut(s) 159, 544, 583
NlaIV GGNNCC 5 cut(s) 85, 169, 276, 283, 301
NmuCI GTSAC 1 cut(s) 287
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 2 cut(s) 159, 583
PciI ACATGT 1 cut(s) 579
PcsI WCGNNNNNNNCGW 3 cut(s) 440, 443, 449
PfeI GAWTC 2 cut(s) 448, 515
Pfl23II CGTACG 1 cut(s) 237
PkrI GCNGC 3 cut(s) 7, 10, 526
PleI GAGTC 2 cut(s) 83, 474
PluTI GGCGCC 1 cut(s) 171
PpsI GAGTC 2 cut(s) 83, 474
PscI ACATGT 1 cut(s) 579
Psp6I CCWGG 2 cut(s) 126, 277
PspGI CCWGG 2 cut(s) 126, 277
PspLI CGTACG 1 cut(s) 237
PspN4I GGNNCC 5 cut(s) 85, 169, 276, 283, 301
PsuI RGATCY 1 cut(s) 335
RsaI GTAC 2 cut(s) 239, 382
RsaNI GTAC 2 cut(s) 238, 381
SatI GCNGC 3 cut(s) 6, 9, 525
Sau3AI GATC 2 cut(s) 322, 335
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 2 cut(s) 84, 474
ScrFI CCNGG 2 cut(s) 128, 279
SetI ASST 5 cut(s) 19, 210, 229, 236, 458
SfaNI GCATC 2 cut(s) 106, 536
SfoI GGCGCC 1 cut(s) 169
Sse9I AATT 1 cut(s) 536
SsiI CCGC 1 cut(s) 303
SspDI GGCGCC 1 cut(s) 167
SspMI CTAG 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 126, 277
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 251, 310
TaqI TCGA 1 cut(s) 78
TaqII GACCGA 1 cut(s) 335
TasI AATT 1 cut(s) 536
TatI WGTACW 1 cut(s) 380
TfiI GAWTC 2 cut(s) 448, 515
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 3 cut(s) 5, 8, 524
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 1 cut(s) 529
XceI RCATGY 2 cut(s) 159, 583
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 1 cut(s) 120
ZrmI AGTACT 1 cut(s) 382
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.