Rh4AG192200

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014577
Physical Location & Seq
Reverse (-)
47318740 .. 47324034
5295 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG192200.1

Sequence Viewer

Length: 597 bp
ATGGCAGCAACACCACCACCTCTGTTCTTCTTCCTCCTGCTCCTCCTAACAACCCTCGTGGGTATCAATACTATCGGAGCCGAAGGAGCCACAACAACTTGGTGCGTGGCGAGGAGCGATGCCAGCAGCCAGGCTCTCCAGACGGCCTTGGACTATGCATGTGCCTCCGGCGCCGACTGTGTTTCAATCCAGTCCGATGGGCTCTGTTACCTCCCCAACACTATCCAAGCACACGCGTCGTACGCTTTCAACAGTTACTACCAGCGCAGGGCTATGGCTCCTGGCTCCTGTGACTTCTCCGGCACCGCCACCGTTGCCCAGACCGATCCCAGTTATGGATCTTGTGTATACCCATCTTCTGTAAGCACGGCTGGAGGGACAAGTACTACACCAACCACAACTCCGACCACAACTACAACCATTCCAAGTCCACCAACAACGACGAATCCGACCTTCGGTAATGGCATTGTGGGTGGATTCACTCCAGTTACTCCAGCTGTCCCCGACGATTCAAAAGCAGAATCCGTTCAGTTTATGCTCCCAACAATCTTGATTCATGTCTCCTTTATTTTTTACTTATTCTTCTCGTTACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

198

Amino Acids

20.61

Weight (kDa)

4.33

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 33 - 104 2.9e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13600
fragaria_vesca FvH4_4g16510 FvH4_4g16510
malus_domestica MD04G1009500.v1.1
prunus_persica Prupe.1G008800_v2.0.a1
pyrus_communis pycom04g00730
rosa_chinensis RchiOBHm_Chr4g0415121
rosa_laevigata RLG00000008096
rosa_multiflora Rmu_sc0000635.1_g000003 Rmu_sc0003748.1_g000016
rosa_roxburghii Rroxscaffold_5G00358920
rosa_rugosa Rorug04G0132400
rosa_samantha Rh4AG192200 Rh4BG190300 Rh4CG203000 Rh4DG190100
rosa_wichuraiana Rw4G016350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 170, 302
AccI GTMKAC 1 cut(s) 348
AccII CGCG 1 cut(s) 236
AciI CCGC 1 cut(s) 306
AclWI GGATC 2 cut(s) 320, 346
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 2 cut(s) 242, 385
AfiI CCNNNNNNNGG 3 cut(s) 268, 335, 455
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 3 cut(s) 186, 250, 513
AjnI CCWGG 2 cut(s) 129, 280
AluBI AGCT 1 cut(s) 497
AluI AGCT 1 cut(s) 497
Alw26I GTCTC 1 cut(s) 565
AlwI GGATC 2 cut(s) 320, 346
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 2 cut(s) 5, 126
Asp700I GAANNNNTTC 1 cut(s) 525
AspLEI GCGC 2 cut(s) 173, 267
BanI GGYRCC 2 cut(s) 170, 302
BanII GRGCYC 1 cut(s) 204
BauI CACGAG 1 cut(s) 56
BbvI GCAGC 2 cut(s) 17, 138
BccI CCATC 2 cut(s) 191, 361
BceAI ACGGC 2 cut(s) 159, 384
BcgI CGANNNNNNTGC 2 cut(s) 219, 253
BciT130I CCWGG 2 cut(s) 131, 282
BcoDI GTCTC 1 cut(s) 565
BfoI RGCGCY 1 cut(s) 174
BisI GCNGC 2 cut(s) 6, 127
BlsI GCNGC 2 cut(s) 7, 128
BmcAI AGTACT 1 cut(s) 385
Bme1390I CCNGG 2 cut(s) 131, 282
BmiI GGNNCC 6 cut(s) 79, 88, 172, 279, 286, 304
BmrFI CCNGG 2 cut(s) 131, 282
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 1 cut(s) 109
BmuI ACTGGG 1 cut(s) 324
BpmI CTGGAG 4 cut(s) 122, 393, 468, 477
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 147
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 335, 455
Bse1I ACTGG 3 cut(s) 190, 330, 485
BseBI CCWGG 2 cut(s) 131, 282
BseDI CCNNGG 1 cut(s) 147
BseLI CCNNNNNNNGG 3 cut(s) 268, 335, 455
BseNI ACTGG 3 cut(s) 190, 330, 485
BseRI GAGGAG 2 cut(s) 32, 127
BseXI GCAGC 2 cut(s) 17, 138
Bsh1236I CGCG 1 cut(s) 236
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 2 cut(s) 170, 302
BsiSI CCGG 2 cut(s) 168, 300
BsiWI CGTACG 1 cut(s) 240
BslFI GGGAC 2 cut(s) 391, 485
BslI CCNNNNNNNGG 3 cut(s) 268, 335, 455
BsmAI GTCTC 1 cut(s) 565
BsmFI GGGAC 2 cut(s) 391, 485
BsnI GGCC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 2 cut(s) 325, 338
BspACI CCGC 1 cut(s) 306
BspANI GGCC 1 cut(s) 146
BspFNI CGCG 1 cut(s) 236
BspLI GGNNCC 6 cut(s) 79, 88, 172, 279, 286, 304
BspPI GGATC 2 cut(s) 320, 346
BspT107I GGYRCC 2 cut(s) 170, 302
BsrI ACTGG 3 cut(s) 190, 330, 485
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 2 cut(s) 325, 338
BssNAI GTATAC 1 cut(s) 349
BssNI GRCGYC 1 cut(s) 171
BssSI CACGAG 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 147
Bst1107I GTATAC 1 cut(s) 349
Bst2BI CACGAG 1 cut(s) 56
Bst2UI CCWGG 2 cut(s) 131, 282
Bst4CI ACNGT 3 cut(s) 179, 254, 313
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 124
BstFNI CGCG 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 2 cut(s) 173, 267
BstKTI GATC 2 cut(s) 328, 341
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 2 cut(s) 325, 338
BstMWI GCNNNNNNNGC 5 cut(s) 86, 123, 170, 242, 314
BstNI CCWGG 2 cut(s) 131, 282
BstNSI RCATGY 1 cut(s) 162
BstSCI CCNGG 2 cut(s) 129, 280
BstUI CGCG 1 cut(s) 236
BstV1I GCAGC 2 cut(s) 17, 138
BstX2I RGATCY 1 cut(s) 338
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 1 cut(s) 338
BstZ17I GTATAC 1 cut(s) 349
BsuRI GGCC 1 cut(s) 146
BtgZI GCGATG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 124
CfoI GCGC 2 cut(s) 173, 267
CseI GACGC 1 cut(s) 225
Csp6I GTAC 2 cut(s) 241, 384
CspCI CAANNNNNGTGG 2 cut(s) 39, 74
CviAII CATG 2 cut(s) 159, 557
CviQI GTAC 2 cut(s) 241, 384
DinI GGCGCC 1 cut(s) 172
DpnI GATC 2 cut(s) 327, 340
DpnII GATC 2 cut(s) 325, 338
Eco130I CCWWGG 1 cut(s) 147
Eco24I GRGCYC 1 cut(s) 204
EcoRII CCWGG 2 cut(s) 129, 280
EcoT14I CCWWGG 1 cut(s) 147
EcoT22I ATGCAT 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 204
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 2 cut(s) 162, 560
FaiI YATR 7 cut(s) 156, 160, 275, 336, 349, 536, 558
FaqI GGGAC 2 cut(s) 391, 485
FatI CATG 2 cut(s) 158, 556
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 2 cut(s) 6, 127
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 2 cut(s) 6, 127
GlaI GCGC 2 cut(s) 172, 266
GluI GCNGC 2 cut(s) 6, 127
GsuI CTGGAG 4 cut(s) 122, 393, 468, 477
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 168, 300
HgaI GACGC 1 cut(s) 225
HhaI GCGC 2 cut(s) 173, 267
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 2 cut(s) 162, 560
Hin6I GCGC 2 cut(s) 171, 265
HinP1I GCGC 2 cut(s) 171, 265
HinfI GANTC 5 cut(s) 445, 477, 509, 521, 553
HpaII CCGG 2 cut(s) 168, 300
Hpy166II GTNNAC 2 cut(s) 349, 431
Hpy188I TCNGA 4 cut(s) 77, 196, 405, 450
Hpy188III TCNNGA 2 cut(s) 139, 550
Hpy8I GTNNAC 2 cut(s) 349, 431
Hpy99I CGWCG 3 cut(s) 241, 445, 509
HpyAV CCTTC 2 cut(s) 77, 463
HpyCH4III ACNGT 3 cut(s) 179, 254, 313
HpyCH4V TGCA 1 cut(s) 158
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 123, 170, 242, 314
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 2 cut(s) 162, 560
HspAI GCGC 2 cut(s) 171, 265
KasI GGCGCC 1 cut(s) 170
Kzo9I GATC 2 cut(s) 325, 338
LmnI GCTCC 7 cut(s) 45, 77, 86, 114, 283, 290, 543
Lsp1109I GCAGC 2 cut(s) 17, 138
LweI GCATC 1 cut(s) 109
MaeIII GTNAC 5 cut(s) 206, 254, 290, 487, 588
MalI GATC 2 cut(s) 327, 340
MboI GATC 2 cut(s) 325, 338
MboII GAAGA 4 cut(s) 19, 22, 348, 574
MflI RGATCY 1 cut(s) 338
MhlI GDGCHC 1 cut(s) 204
MluI ACGCGT 1 cut(s) 234
Mly113I GGCGCC 1 cut(s) 171
MmeI TCCRAC 2 cut(s) 428, 473
MnlI CCTC 8 cut(s) 30, 44, 53, 65, 105, 175, 221, 368
Mph1103I ATGCAT 1 cut(s) 160
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 1 cut(s) 595
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 2 cut(s) 168, 300
MspR9I CCNGG 2 cut(s) 131, 282
MvaI CCWGG 2 cut(s) 131, 282
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 5 cut(s) 86, 123, 170, 242, 314
NarI GGCGCC 1 cut(s) 171
NdeII GATC 2 cut(s) 325, 338
NlaIII CATG 2 cut(s) 162, 560
NlaIV GGNNCC 6 cut(s) 79, 88, 172, 279, 286, 304
NmuCI GTSAC 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 1 cut(s) 162
PcsI WCGNNNNNNNCGW 1 cut(s) 446
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 5 cut(s) 445, 477, 509, 521, 553
Pfl23II CGTACG 1 cut(s) 240
PkrI GCNGC 2 cut(s) 7, 128
PluTI GGCGCC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 129, 280
PspGI CCWGG 2 cut(s) 129, 280
PspLI CGTACG 1 cut(s) 240
PspN4I GGNNCC 6 cut(s) 79, 88, 172, 279, 286, 304
PsuI RGATCY 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 2 cut(s) 242, 385
RsaNI GTAC 2 cut(s) 241, 384
SaqAI TTAA 1 cut(s) 595
SatI GCNGC 2 cut(s) 6, 127
Sau3AI GATC 2 cut(s) 325, 338
ScaI AGTACT 1 cut(s) 385
ScrFI CCNGG 2 cut(s) 131, 282
SduI GDGCHC 1 cut(s) 204
SetI ASST 5 cut(s) 22, 213, 455, 499, 595
SfaNI GCATC 1 cut(s) 109
SfoI GGCGCC 1 cut(s) 172
SsiI CCGC 1 cut(s) 306
SspDI GGCGCC 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 129, 280
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 3 cut(s) 179, 254, 313
TaqII GACCGA 1 cut(s) 338
TatI WGTACW 1 cut(s) 383
TfiI GAWTC 5 cut(s) 445, 477, 509, 521, 553
Tru1I TTAA 1 cut(s) 595
Tru9I TTAA 1 cut(s) 595
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 2 cut(s) 5, 126
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 545
TspGWI ACGGA 1 cut(s) 514
XceI RCATGY 1 cut(s) 162
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 525
ZrmI AGTACT 1 cut(s) 385
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.