Rh4DG190100

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014577
Physical Location & Seq
Reverse (-)
37410626 .. 37413527
2902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG190100.1

Sequence Viewer

Length: 465 bp
ATGGCAGCAACACCACCACCTCTGTTCTTCTTCCTCCTCCTCCTAACAACCCTCGTGGGTATCAATACTATCGGAGCCGAAGGAGCCACAACAACTTGGTGCGTGGCGAGGAGCGATGCCAGCAGCCAGGCTCTCCAGACGGCCTTGGACTATGCATGTGCCTCCGGCGCCGACTGTGTTTCAATCCAGTCCGATGGGCTCTGTTACCTCCCCAACACTATCCAAGCTCACGCGTCGTACGCTTTCAACAGTTACTACCAGCGCAGGGCTATGGCTCCTGGCTCCTGTGACTTCTCCGGCACAGCCACCGTTGCCCAGACTGATCCCAGTTATGGATCTTGTGTATACCCATCTTCTGTAAGGTATGGTTACAGTCTCTCAACAATCTTATTGGCTTTAGCTATGGGAATGTTCGTAATGTCTTCTTTTTTGACTGGTTCTTCTGGTCGGTATCATTCCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

154

Amino Acids

16.23

Weight (kDa)

5.36

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 32 - 103 1.6e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13600
fragaria_vesca FvH4_4g16510 FvH4_4g16510
malus_domestica MD04G1009500.v1.1
prunus_persica Prupe.1G008800_v2.0.a1
pyrus_communis pycom04g00730
rosa_chinensis RchiOBHm_Chr4g0415121
rosa_laevigata RLG00000008096
rosa_multiflora Rmu_sc0000635.1_g000003 Rmu_sc0003748.1_g000016
rosa_roxburghii Rroxscaffold_5G00358920
rosa_rugosa Rorug04G0132400
rosa_samantha Rh4AG192200 Rh4BG190300 Rh4CG203000 Rh4DG190100
rosa_wichuraiana Rw4G016350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 233
AclWI GGATC 2 cut(s) 317, 343
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 2 cut(s) 265, 332
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 2 cut(s) 183, 247
AjnI CCWGG 2 cut(s) 126, 277
AluBI AGCT 2 cut(s) 227, 401
AluI AGCT 2 cut(s) 227, 401
Alw26I GTCTC 1 cut(s) 380
AlwI GGATC 2 cut(s) 317, 343
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 5, 123
AspLEI GCGC 2 cut(s) 170, 264
BanI GGYRCC 1 cut(s) 167
BanII GRGCYC 1 cut(s) 201
BauI CACGAG 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 414
BbvI GCAGC 2 cut(s) 17, 135
BccI CCATC 2 cut(s) 188, 358
BceAI ACGGC 1 cut(s) 156
BciT130I CCWGG 2 cut(s) 128, 279
BcoDI GTCTC 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 2 cut(s) 6, 124
BlsI GCNGC 2 cut(s) 7, 125
Bme1390I CCNGG 2 cut(s) 128, 279
BmiI GGNNCC 5 cut(s) 76, 85, 169, 276, 283
BmrFI CCNGG 2 cut(s) 128, 279
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 106
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 414
BpmI CTGGAG 1 cut(s) 119
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 144
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 332
Bse1I ACTGG 3 cut(s) 187, 327, 439
BseBI CCWGG 2 cut(s) 128, 279
BseDI CCNNGG 1 cut(s) 144
BseLI CCNNNNNNNGG 2 cut(s) 265, 332
BseNI ACTGG 3 cut(s) 187, 327, 439
BseRI GAGGAG 3 cut(s) 26, 29, 124
BseXI GCAGC 2 cut(s) 17, 135
Bsh1236I CGCG 1 cut(s) 233
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 167
BsiSI CCGG 2 cut(s) 165, 297
BsiWI CGTACG 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 265, 332
BsmAI GTCTC 1 cut(s) 380
BsnI GGCC 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 322, 335
BspANI GGCC 1 cut(s) 143
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 5 cut(s) 76, 85, 169, 276, 283
BspPI GGATC 2 cut(s) 317, 343
BspT107I GGYRCC 1 cut(s) 167
BsrI ACTGG 3 cut(s) 187, 327, 439
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 2 cut(s) 322, 335
BssNAI GTATAC 1 cut(s) 346
BssNI GRCGYC 1 cut(s) 168
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 144
Bst1107I GTATAC 1 cut(s) 346
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 128, 279
Bst4CI ACNGT 4 cut(s) 176, 251, 310, 374
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 121
BstFNI CGCG 1 cut(s) 233
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 170, 264
BstKTI GATC 2 cut(s) 325, 338
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 2 cut(s) 322, 335
BstMWI GCNNNNNNNGC 5 cut(s) 83, 120, 167, 239, 311
BstNI CCWGG 2 cut(s) 128, 279
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 126, 277
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 2 cut(s) 17, 135
BstV2I GAAGAC 1 cut(s) 414
BstX2I RGATCY 1 cut(s) 335
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 1 cut(s) 335
BstZ17I GTATAC 1 cut(s) 346
BsuRI GGCC 1 cut(s) 143
BtgZI GCGATG 1 cut(s) 129
Cac8I GCNNGC 1 cut(s) 121
CfoI GCGC 2 cut(s) 170, 264
CseI GACGC 1 cut(s) 222
Csp6I GTAC 1 cut(s) 238
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviAII CATG 1 cut(s) 156
CviQI GTAC 1 cut(s) 238
DinI GGCGCC 1 cut(s) 169
DpnI GATC 2 cut(s) 324, 337
DpnII GATC 2 cut(s) 322, 335
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 126, 277
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 201
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 1 cut(s) 159
FaiI YATR 7 cut(s) 153, 157, 272, 333, 346, 366, 404
FatI CATG 1 cut(s) 155
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 2 cut(s) 6, 124
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 2 cut(s) 6, 124
GlaI GCGC 2 cut(s) 169, 263
GluI GCNGC 2 cut(s) 6, 124
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 143
HapII CCGG 2 cut(s) 165, 297
HgaI GACGC 1 cut(s) 222
HhaI GCGC 2 cut(s) 170, 264
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 1 cut(s) 159
Hin6I GCGC 2 cut(s) 168, 262
HinP1I GCGC 2 cut(s) 168, 262
HpaII CCGG 2 cut(s) 165, 297
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 2 cut(s) 74, 193
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 1 cut(s) 74
HpyCH4III ACNGT 4 cut(s) 176, 251, 310, 374
HpyCH4V TGCA 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 120, 167, 239, 311
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 1 cut(s) 159
HspAI GCGC 2 cut(s) 168, 262
KasI GGCGCC 1 cut(s) 167
Kzo9I GATC 2 cut(s) 322, 335
LmnI GCTCC 5 cut(s) 74, 83, 111, 280, 287
Lsp1109I GCAGC 2 cut(s) 17, 135
LweI GCATC 1 cut(s) 106
MaeIII GTNAC 4 cut(s) 203, 251, 287, 368
MalI GATC 2 cut(s) 324, 337
MboI GATC 2 cut(s) 322, 335
MboII GAAGA 5 cut(s) 19, 22, 345, 414, 432
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 201
MluI ACGCGT 1 cut(s) 231
Mly113I GGCGCC 1 cut(s) 168
MnlI CCTC 8 cut(s) 30, 44, 47, 50, 62, 102, 172, 218
Mph1103I ATGCAT 1 cut(s) 157
MspI CCGG 2 cut(s) 165, 297
MspR9I CCNGG 2 cut(s) 128, 279
MvaI CCWGG 2 cut(s) 128, 279
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 5 cut(s) 83, 120, 167, 239, 311
NarI GGCGCC 1 cut(s) 168
NdeII GATC 2 cut(s) 322, 335
NlaIII CATG 1 cut(s) 159
NlaIV GGNNCC 5 cut(s) 76, 85, 169, 276, 283
NmuCI GTSAC 1 cut(s) 287
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 1 cut(s) 159
Pfl23II CGTACG 1 cut(s) 237
PkrI GCNGC 2 cut(s) 7, 125
PluTI GGCGCC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 126, 277
PspGI CCWGG 2 cut(s) 126, 277
PspLI CGTACG 1 cut(s) 237
PspN4I GGNNCC 5 cut(s) 76, 85, 169, 276, 283
PsuI RGATCY 1 cut(s) 335
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SatI GCNGC 2 cut(s) 6, 124
Sau3AI GATC 2 cut(s) 322, 335
ScrFI CCNGG 2 cut(s) 128, 279
SduI GDGCHC 1 cut(s) 201
SetI ASST 5 cut(s) 22, 210, 229, 365, 403
SfaNI GCATC 1 cut(s) 106
SfoI GGCGCC 1 cut(s) 169
SspDI GGCGCC 1 cut(s) 167
StyD4I CCNGG 2 cut(s) 126, 277
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 4 cut(s) 176, 251, 310, 374
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 2 cut(s) 5, 123
Tsp45I GTSAC 1 cut(s) 287
XceI RCATGY 1 cut(s) 159
XmiI GTMKAC 1 cut(s) 345
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.