MD04G1009500.v1.1

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014577
Physical Location & Seq
Forward (+)
1094586 .. 1097466
2881 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1009500.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGCTCCACTCTCACTCTCCAACCAGCACCCATTAATCTCCTTCCTCTTCTTCCTCACACCCATTGTCATGGTGGTGGGCATGCTTGGAGTTTTCGGGGTGGACGGAGCAAATTCTCCGACGTCTTCCAGCACAACATGGTGTGTGGCTAGGAGTGATGCGAGCGCACAGGCTCTTCAGACAGCCTTGGACTATGCATGCGCAGCTGGTGCGGACTGTTCTCCAATCCAATCCGACGGACTATGTTATCTCCCAAACACCATCCAAGCTCATGCCTCTTATGCCTTCAACAGTTACTACCAGCGCCAGGCTAAGGCCCCTGGTTCTTGCGGCTTCTCCGGCACTGCCACCATTGCCCAAACAGACCCCAGTTATGGATCTTGTGATTACCCATCTTCCACAAGCGCGGCTGGCGGGACAACTTCACCTTCCACACCACCAACAGTTCCGAATCCACAAACGCCGCCATCAACATTCGCACCACCCTTCACCGCCTCCACCGGCGGATTCACTCCTGGATCGACCCCGATGATCCCTGACGATAACTCAAAAGCGTCCCTCGACTCCATGTCCCCAACAATCTTGATGCCCATTTCCCTTGTGCTCGTTCTTTTATTCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

208

Amino Acids

21.22

Weight (kDa)

4.25

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 46 - 117 4.7e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13600
fragaria_vesca FvH4_4g16510 FvH4_4g16510
malus_domestica MD04G1009500.v1.1
prunus_persica Prupe.1G008800_v2.0.a1
pyrus_communis pycom04g00730
rosa_chinensis RchiOBHm_Chr4g0415121
rosa_laevigata RLG00000008096
rosa_multiflora Rmu_sc0000635.1_g000003 Rmu_sc0003748.1_g000016
rosa_roxburghii Rroxscaffold_5G00358920
rosa_rugosa Rorug04G0132400
rosa_samantha Rh4AG192200 Rh4BG190300 Rh4CG203000 Rh4DG190100
rosa_wichuraiana Rw4G016350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 125
Acc16I TGCGCA 1 cut(s) 202
AccII CGCG 1 cut(s) 407
AciI CCGC 7 cut(s) 212, 330, 407, 414, 464, 492, 504
AclWI GGATC 3 cut(s) 385, 526, 526
AcsI RAATTY 1 cut(s) 112
AcuI CTGAAG 1 cut(s) 161
AcyI GRCGYC 1 cut(s) 122
AfiI CCNNNNNNNGG 3 cut(s) 307, 313, 374
AgsI TTSAA 1 cut(s) 289
AhdI GACNNNNNGTC 1 cut(s) 568
AjnI CCWGG 3 cut(s) 306, 319, 514
AluBI AGCT 2 cut(s) 206, 269
AluI AGCT 2 cut(s) 206, 269
Alw21I GWGCWC 1 cut(s) 606
AlwI GGATC 3 cut(s) 385, 526, 526
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 1 cut(s) 112
AseI ATTAAT 1 cut(s) 35
AspLEI GCGC 4 cut(s) 167, 203, 306, 407
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 3 cut(s) 417, 481, 610
BbsI GAAGAC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 606
BbvI GCAGC 1 cut(s) 215
BccI CCATC 3 cut(s) 269, 400, 475
BciT130I CCWGG 3 cut(s) 308, 321, 516
BfaI CTAG 1 cut(s) 150
BfoI RGCGCY 1 cut(s) 307
BisI GCNGC 4 cut(s) 204, 331, 408, 464
BlsI GCNGC 4 cut(s) 205, 332, 409, 465
Bme1390I CCNGG 3 cut(s) 308, 321, 516
BmeRI GACNNNNNGTC 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 2 cut(s) 6, 318
BmrFI CCNGG 3 cut(s) 308, 321, 516
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 2 cut(s) 148, 576
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 186, 319
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 313, 374
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 1 cut(s) 369
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 3 cut(s) 308, 321, 516
BseDI CCNNGG 2 cut(s) 186, 319
BseGI GGATG 1 cut(s) 261
BseLI CCNNNNNNNGG 3 cut(s) 307, 313, 374
BseMI GCAATG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 369
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 407
BshFI GGCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 606
BsiSI CCGG 2 cut(s) 339, 501
BslFI GGGAC 3 cut(s) 430, 541, 556
BslI CCNNNNNNNGG 3 cut(s) 307, 313, 374
BsmFI GGGAC 3 cut(s) 430, 541, 556
BsnI GGCC 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 606
Bsp143I GATC 3 cut(s) 377, 518, 531
BspACI CCGC 7 cut(s) 212, 330, 407, 414, 464, 492, 504
BspANI GGCC 1 cut(s) 317
BspFNI CGCG 1 cut(s) 407
BspLI GGNNCC 2 cut(s) 6, 318
BspPI GGATC 3 cut(s) 385, 526, 526
BspQI GCTCTTC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 351
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 1 cut(s) 369
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 2 cut(s) 186, 319
BssMI GATC 3 cut(s) 377, 518, 531
BssNI GRCGYC 1 cut(s) 122
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 308, 321, 516
Bst4CI ACNGT 3 cut(s) 218, 293, 445
Bst6I CTCTTC 2 cut(s) 53, 180
BstACI GRCGYC 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 4 cut(s) 83, 163, 199, 412
BstDEI CTNAG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 261
BstFNI CGCG 1 cut(s) 407
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 4 cut(s) 167, 203, 306, 407
BstKTI GATC 3 cut(s) 380, 521, 534
BstMBI GATC 3 cut(s) 377, 518, 531
BstMWI GCNNNNNNNGC 6 cut(s) 203, 209, 281, 339, 353, 411
BstNI CCWGG 3 cut(s) 308, 321, 516
BstNSI RCATGY 2 cut(s) 85, 201
BstSCI CCNGG 3 cut(s) 306, 319, 514
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 1 cut(s) 215
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 377
BstXI CCANNNNNNTGG 1 cut(s) 70
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 1 cut(s) 317
BtsCI GGATG 1 cut(s) 261
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 4 cut(s) 83, 163, 199, 412
CfoI GCGC 4 cut(s) 167, 203, 306, 407
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 316
CseI GACGC 1 cut(s) 543
CviAII CATG 6 cut(s) 70, 82, 138, 198, 272, 568
DdeI CTNAG 1 cut(s) 312
DpnI GATC 3 cut(s) 379, 520, 533
DpnII GATC 3 cut(s) 377, 518, 531
DriI GACNNNNNGTC 1 cut(s) 568
Eam1104I CTCTTC 2 cut(s) 53, 180
Eam1105I GACNNNNNGTC 1 cut(s) 568
EarI CTCTTC 2 cut(s) 53, 180
EciI GGCGGA 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 161
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 3 cut(s) 306, 319, 514
EcoT14I CCWWGG 1 cut(s) 186
EcoT22I ATGCAT 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 6 cut(s) 73, 85, 141, 201, 275, 571
FaqI GGGAC 3 cut(s) 430, 541, 556
FatI CATG 6 cut(s) 69, 81, 137, 197, 271, 567
FauI CCCGC 1 cut(s) 407
Fnu4HI GCNGC 4 cut(s) 204, 331, 408, 464
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 4 cut(s) 204, 331, 408, 464
FspBI CTAG 1 cut(s) 150
FspI TGCGCA 1 cut(s) 202
GlaI GCGC 4 cut(s) 166, 202, 305, 406
GluI GCNGC 4 cut(s) 204, 331, 408, 464
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 1 cut(s) 317
HapII CCGG 2 cut(s) 339, 501
HgaI GACGC 1 cut(s) 543
HhaI GCGC 4 cut(s) 167, 203, 306, 407
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 6 cut(s) 73, 85, 141, 201, 275, 571
Hin6I GCGC 4 cut(s) 165, 201, 304, 405
HinP1I GCGC 4 cut(s) 165, 201, 304, 405
HinfI GANTC 3 cut(s) 451, 507, 563
HpaII CCGG 2 cut(s) 339, 501
HphI GGTGA 3 cut(s) 417, 481, 610
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 4 cut(s) 120, 180, 235, 450
Hpy188III TCNNGA 1 cut(s) 583
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 2 cut(s) 124, 239
HpyAV CCTTC 4 cut(s) 52, 295, 438, 496
HpyCH4III ACNGT 3 cut(s) 218, 293, 445
HpyCH4IV ACGT 1 cut(s) 122
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 6 cut(s) 203, 209, 281, 339, 353, 411
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 1 cut(s) 122
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 6 cut(s) 73, 85, 141, 201, 275, 571
HspAI GCGC 4 cut(s) 165, 201, 304, 405
Kzo9I GATC 3 cut(s) 377, 518, 531
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 10, 107
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 2 cut(s) 148, 576
MaeI CTAG 1 cut(s) 150
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 1 cut(s) 293
MalI GATC 3 cut(s) 379, 520, 533
MboI GATC 3 cut(s) 377, 518, 531
MboII GAAGA 5 cut(s) 40, 43, 117, 167, 387
MflI RGATCY 1 cut(s) 377
MhlI GDGCHC 1 cut(s) 606
MluCI AATT 1 cut(s) 112
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 3 cut(s) 45, 143, 258
MnlI CCTC 5 cut(s) 56, 65, 286, 505, 569
Mph1103I ATGCAT 1 cut(s) 199
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 2 cut(s) 68, 74
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 2 cut(s) 339, 501
MspR9I CCNGG 3 cut(s) 308, 321, 516
MvaI CCWGG 3 cut(s) 308, 321, 516
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 6 cut(s) 203, 209, 281, 339, 353, 411
NdeII GATC 3 cut(s) 377, 518, 531
NlaIII CATG 6 cut(s) 73, 85, 141, 201, 275, 571
NlaIV GGNNCC 2 cut(s) 6, 318
NsbI TGCGCA 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 199
NspI RCATGY 2 cut(s) 85, 201
PaeI GCATGC 2 cut(s) 85, 201
PciSI GCTCTTC 1 cut(s) 180
PfeI GAWTC 2 cut(s) 451, 507
PfoI TCCNGGA 1 cut(s) 514
PkrI GCNGC 4 cut(s) 205, 332, 409, 465
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
PshBI ATTAAT 1 cut(s) 35
Psp6I CCWGG 3 cut(s) 306, 319, 514
PspGI CCWGG 3 cut(s) 306, 319, 514
PspN4I GGNNCC 2 cut(s) 6, 318
PspPI GGNCC 1 cut(s) 316
PsuI RGATCY 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 206
RseI CAYNNNNRTG 2 cut(s) 68, 74
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 4 cut(s) 204, 331, 408, 464
Sau3AI GATC 3 cut(s) 377, 518, 531
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 3 cut(s) 308, 321, 516
SduI GDGCHC 1 cut(s) 606
SetI ASST 5 cut(s) 125, 208, 271, 430, 623
SfaNI GCATC 2 cut(s) 148, 576
SgrAI CRCCGGYG 1 cut(s) 500
SmiMI CAYNNNNRTG 2 cut(s) 68, 74
SphI GCATGC 2 cut(s) 85, 201
Sse9I AATT 1 cut(s) 112
SsiI CCGC 7 cut(s) 212, 330, 407, 414, 464, 492, 504
SspMI CTAG 1 cut(s) 150
StyD4I CCNGG 3 cut(s) 306, 319, 514
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 3 cut(s) 218, 293, 445
TaiI ACGT 1 cut(s) 125
TaqI TCGA 2 cut(s) 521, 561
TasI AATT 1 cut(s) 112
TauI GCSGC 3 cut(s) 333, 410, 466
TfiI GAWTC 2 cut(s) 451, 507
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 349
TseI GCWGC 1 cut(s) 203
TspGWI ACGGA 2 cut(s) 120, 252
TspRI CASTG 1 cut(s) 349
VspI ATTAAT 1 cut(s) 35
XapI RAATTY 1 cut(s) 112
XceI RCATGY 2 cut(s) 85, 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XspI CTAG 1 cut(s) 150
ZraI GACGTC 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.