Rh4BG190300

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014577
Physical Location & Seq
Reverse (-)
33012536 .. 33015146
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG190300.1

Sequence Viewer

Length: 582 bp
ATGGCAGCAACACCACCACCTCTGTTCTTCTTCCTCCTCCTAACAATCCTCGTGGGTATCAACACTATCGGAGCCGAAGGAGCCACAACAACTTGGTGCGTGGCGAGGAGCGATGCCAGCAGCCAGGCTCTCCAGACGGCCTTGGACTATGCATGTGCCTCCGGCGCCGACTGTGTTTCAATCCAGTCCGATGGGCTCTGTTACCTCCCCAACACTATCCAAGCTCACGCGTCGTACGCTTTCAACAGTTACTACCAGCGCAGGGCTATGGCTCCTGGCTCCTGTGACTTCTCCGGCACCGCCACCGTTGCCCAGACCGATCCCAGTTATGGATCTTGTGTATACCCATCTTCTGTAAGCACGGCTGGAGGGACAAGTACTACACCAACCACAACTCCGACCACAACTACAACCATTCCAAGTCCACCAACAACGACGAATCCGACCTTCGGCAATGGCGTTGTGGGTGGATTCACTCCAGTTACTCCAGCTGTCCCCGACGATTCAAAAGCAGAATCCGTTCTGTTTATGCTCCCAACAATCTTGATTCATGTCTCCTTTATTTTTTACTTATTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

193

Amino Acids

20.07

Weight (kDa)

4.33

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 31 - 102 2.7e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13600
fragaria_vesca FvH4_4g16510 FvH4_4g16510
malus_domestica MD04G1009500.v1.1
prunus_persica Prupe.1G008800_v2.0.a1
pyrus_communis pycom04g00730
rosa_chinensis RchiOBHm_Chr4g0415121
rosa_laevigata RLG00000008096
rosa_multiflora Rmu_sc0000635.1_g000003 Rmu_sc0003748.1_g000016
rosa_roxburghii Rroxscaffold_5G00358920
rosa_rugosa Rorug04G0132400
rosa_samantha Rh4AG192200 Rh4BG190300 Rh4CG203000 Rh4DG190100
rosa_wichuraiana Rw4G016350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 164, 296
AccI GTMKAC 1 cut(s) 342
AccII CGCG 1 cut(s) 230
AciI CCGC 1 cut(s) 300
AclWI GGATC 2 cut(s) 314, 340
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 2 cut(s) 236, 379
AfiI CCNNNNNNNGG 3 cut(s) 262, 329, 449
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 3 cut(s) 180, 244, 507
AjnI CCWGG 2 cut(s) 123, 274
AluBI AGCT 2 cut(s) 224, 491
AluI AGCT 2 cut(s) 224, 491
Alw26I GTCTC 1 cut(s) 559
AlwI GGATC 2 cut(s) 314, 340
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 5, 120
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 2 cut(s) 167, 261
BanI GGYRCC 2 cut(s) 164, 296
BanII GRGCYC 1 cut(s) 198
BauI CACGAG 1 cut(s) 50
BbvI GCAGC 2 cut(s) 17, 132
BccI CCATC 2 cut(s) 185, 355
BceAI ACGGC 2 cut(s) 153, 378
BciT130I CCWGG 2 cut(s) 125, 276
BcoDI GTCTC 1 cut(s) 559
BfaI CTAG 1 cut(s) 580
BfoI RGCGCY 1 cut(s) 168
BisI GCNGC 2 cut(s) 6, 121
BlsI GCNGC 2 cut(s) 7, 122
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 2 cut(s) 125, 276
BmiI GGNNCC 6 cut(s) 73, 82, 166, 273, 280, 298
BmrFI CCNGG 2 cut(s) 125, 276
BmrI ACTGGG 1 cut(s) 318
BmsI GCATC 1 cut(s) 103
BmuI ACTGGG 1 cut(s) 318
BpmI CTGGAG 4 cut(s) 116, 387, 462, 471
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 329, 449
Bse1I ACTGG 3 cut(s) 184, 324, 479
Bse3DI GCAATG 1 cut(s) 460
BseBI CCWGG 2 cut(s) 125, 276
BseDI CCNNGG 1 cut(s) 141
BseLI CCNNNNNNNGG 3 cut(s) 262, 329, 449
BseMI GCAATG 1 cut(s) 460
BseNI ACTGG 3 cut(s) 184, 324, 479
BseRI GAGGAG 2 cut(s) 26, 121
BseXI GCAGC 2 cut(s) 17, 132
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 2 cut(s) 164, 296
BsiSI CCGG 2 cut(s) 162, 294
BsiWI CGTACG 1 cut(s) 234
BslFI GGGAC 2 cut(s) 385, 479
BslI CCNNNNNNNGG 3 cut(s) 262, 329, 449
BsmAI GTCTC 1 cut(s) 559
BsmFI GGGAC 2 cut(s) 385, 479
BsnI GGCC 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 319, 332
BspACI CCGC 1 cut(s) 300
BspANI GGCC 1 cut(s) 140
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 6 cut(s) 73, 82, 166, 273, 280, 298
BspPI GGATC 2 cut(s) 314, 340
BspT107I GGYRCC 2 cut(s) 164, 296
BsrDI GCAATG 1 cut(s) 460
BsrI ACTGG 3 cut(s) 184, 324, 479
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 2 cut(s) 319, 332
BssNAI GTATAC 1 cut(s) 343
BssNI GRCGYC 1 cut(s) 165
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 141
Bst1107I GTATAC 1 cut(s) 343
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 125, 276
Bst4CI ACNGT 3 cut(s) 173, 248, 307
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 118
BstFNI CGCG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 2 cut(s) 167, 261
BstKTI GATC 2 cut(s) 322, 335
BstMAI GTCTC 1 cut(s) 559
BstMBI GATC 2 cut(s) 319, 332
BstMWI GCNNNNNNNGC 5 cut(s) 80, 117, 164, 236, 308
BstNI CCWGG 2 cut(s) 125, 276
BstNSI RCATGY 1 cut(s) 156
BstSCI CCNGG 2 cut(s) 123, 274
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 2 cut(s) 17, 132
BstX2I RGATCY 1 cut(s) 332
BstXI CCANNNNNNTGG 1 cut(s) 191
BstYI RGATCY 1 cut(s) 332
BstZ17I GTATAC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 140
BtgZI GCGATG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 2 cut(s) 167, 261
CseI GACGC 1 cut(s) 219
Csp6I GTAC 2 cut(s) 235, 378
CspCI CAANNNNNGTGG 2 cut(s) 33, 68
CviAII CATG 2 cut(s) 153, 551
CviQI GTAC 2 cut(s) 235, 378
DinI GGCGCC 1 cut(s) 166
DpnI GATC 2 cut(s) 321, 334
DpnII GATC 2 cut(s) 319, 332
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 198
EcoRII CCWGG 2 cut(s) 123, 274
EcoT14I CCWWGG 1 cut(s) 141
EcoT22I ATGCAT 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 198
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 2 cut(s) 156, 554
FaiI YATR 7 cut(s) 150, 154, 269, 330, 343, 530, 552
FaqI GGGAC 2 cut(s) 385, 479
FatI CATG 2 cut(s) 152, 550
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 2 cut(s) 6, 121
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 2 cut(s) 6, 121
FspBI CTAG 1 cut(s) 580
GlaI GCGC 2 cut(s) 166, 260
GluI GCNGC 2 cut(s) 6, 121
GsuI CTGGAG 4 cut(s) 116, 387, 462, 471
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 1 cut(s) 140
HapII CCGG 2 cut(s) 162, 294
HgaI GACGC 1 cut(s) 219
HhaI GCGC 2 cut(s) 167, 261
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 2 cut(s) 156, 554
Hin6I GCGC 2 cut(s) 165, 259
HinP1I GCGC 2 cut(s) 165, 259
HinfI GANTC 5 cut(s) 439, 471, 503, 515, 547
HpaII CCGG 2 cut(s) 162, 294
Hpy166II GTNNAC 2 cut(s) 343, 425
Hpy188I TCNGA 4 cut(s) 71, 190, 399, 444
Hpy188III TCNNGA 2 cut(s) 133, 544
Hpy8I GTNNAC 2 cut(s) 343, 425
Hpy99I CGWCG 3 cut(s) 235, 439, 503
HpyAV CCTTC 2 cut(s) 71, 457
HpyCH4III ACNGT 3 cut(s) 173, 248, 307
HpyCH4V TGCA 1 cut(s) 152
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 117, 164, 236, 308
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 2 cut(s) 156, 554
HspAI GCGC 2 cut(s) 165, 259
KasI GGCGCC 1 cut(s) 164
Kzo9I GATC 2 cut(s) 319, 332
LmnI GCTCC 6 cut(s) 71, 80, 108, 277, 284, 537
Lsp1109I GCAGC 2 cut(s) 17, 132
LweI GCATC 1 cut(s) 103
MaeI CTAG 1 cut(s) 580
MaeIII GTNAC 4 cut(s) 200, 248, 284, 481
MalI GATC 2 cut(s) 321, 334
MboI GATC 2 cut(s) 319, 332
MboII GAAGA 4 cut(s) 19, 22, 342, 568
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 1 cut(s) 198
MluI ACGCGT 1 cut(s) 228
Mly113I GGCGCC 1 cut(s) 165
MmeI TCCRAC 2 cut(s) 422, 467
MnlI CCTC 8 cut(s) 30, 44, 47, 59, 99, 169, 215, 362
Mph1103I ATGCAT 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 2 cut(s) 162, 294
MspR9I CCNGG 2 cut(s) 125, 276
MvaI CCWGG 2 cut(s) 125, 276
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 5 cut(s) 80, 117, 164, 236, 308
NarI GGCGCC 1 cut(s) 165
NdeII GATC 2 cut(s) 319, 332
NlaIII CATG 2 cut(s) 156, 554
NlaIV GGNNCC 6 cut(s) 73, 82, 166, 273, 280, 298
NmuCI GTSAC 1 cut(s) 284
NsiI ATGCAT 1 cut(s) 154
NspI RCATGY 1 cut(s) 156
PcsI WCGNNNNNNNCGW 2 cut(s) 440, 456
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 5 cut(s) 439, 471, 503, 515, 547
Pfl23II CGTACG 1 cut(s) 234
PkrI GCNGC 2 cut(s) 7, 122
PluTI GGCGCC 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 123, 274
PspGI CCWGG 2 cut(s) 123, 274
PspLI CGTACG 1 cut(s) 234
PspN4I GGNNCC 6 cut(s) 73, 82, 166, 273, 280, 298
PsuI RGATCY 1 cut(s) 332
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 2 cut(s) 236, 379
RsaNI GTAC 2 cut(s) 235, 378
SatI GCNGC 2 cut(s) 6, 121
Sau3AI GATC 2 cut(s) 319, 332
ScaI AGTACT 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 125, 276
SduI GDGCHC 1 cut(s) 198
SetI ASST 5 cut(s) 22, 207, 226, 449, 493
SfaNI GCATC 1 cut(s) 103
SfoI GGCGCC 1 cut(s) 166
SsiI CCGC 1 cut(s) 300
SspDI GGCGCC 1 cut(s) 164
SspMI CTAG 1 cut(s) 580
StyD4I CCNGG 2 cut(s) 123, 274
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 3 cut(s) 173, 248, 307
TaqII GACCGA 1 cut(s) 332
TatI WGTACW 1 cut(s) 377
TfiI GAWTC 5 cut(s) 439, 471, 503, 515, 547
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 2 cut(s) 5, 120
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 1 cut(s) 539
TspGWI ACGGA 1 cut(s) 508
XceI RCATGY 1 cut(s) 156
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 1 cut(s) 580
ZrmI AGTACT 1 cut(s) 379
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.