RLG00000008096

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014577
Physical Location & Seq
Forward (+)
25567341 .. 25570349
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008096

Sequence Viewer

Length: 585 bp
ATGGCAGCAACACCACCACCTCTGTTCTTCTTCCTCCTCCTCCTAACAACCCTCGTGGTTATCAACACTATCGGAGCCGAAGGAGCCACAACAACTTGGTGCGTGGCGAGGAGCGATGCCAGCAGCCAGGCTCTCCAGACGGCCTTGGACTATGCATGTGCCTCCGGCGCCGATTGTGTTTCAATCCAGTCCGATGGGCTCTGTTACCTCCCCAACACTATCCAAGCTCACGCGTCGTACGCTTTCAACAGTTACTACCAGCGCAGGGCTATGGCTCCTGGCTCCTGTGACTTCTCCGGCACCGCCACCGTTGCCCAGACCGATCCCAGTTATGGATCTTGTGTATACCCATCTTCTGTAAGCACGGCTGGAGGGACAAGTACTACACCAACCACAACTCCGACCACAACTACAACCATTCCAAGTCCACCAACAATGACGAATCCGACCTTCGGCAATGGCGTTGTGGGTGGATTCACTCCAGTTACTCCAGCTGTCCCCGACGATTCAAAAGCAGCATCCTTTCAGTTCATGCTCCCAACAATCTTGATTCCTGTCTCCTTTATTTTTTACTTATTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

195

Amino Acids

20.21

Weight (kDa)

4.22

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 32 - 103 2.7e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014577)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13600
fragaria_vesca FvH4_4g16510 FvH4_4g16510
malus_domestica MD04G1009500.v1.1
prunus_persica Prupe.1G008800_v2.0.a1
pyrus_communis pycom04g00730
rosa_chinensis RchiOBHm_Chr4g0415121
rosa_laevigata RLG00000008096
rosa_multiflora Rmu_sc0000635.1_g000003 Rmu_sc0003748.1_g000016
rosa_roxburghii Rroxscaffold_5G00358920
rosa_rugosa Rorug04G0132400
rosa_samantha Rh4AG192200 Rh4BG190300 Rh4CG203000 Rh4DG190100
rosa_wichuraiana Rw4G016350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 167, 299
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 233
AciI CCGC 1 cut(s) 303
AclWI GGATC 2 cut(s) 317, 343
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 2 cut(s) 239, 382
AfiI CCNNNNNNNGG 3 cut(s) 265, 332, 452
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 3 cut(s) 183, 247, 510
AjnI CCWGG 2 cut(s) 126, 277
AluBI AGCT 2 cut(s) 227, 494
AluI AGCT 2 cut(s) 227, 494
Alw26I GTCTC 1 cut(s) 562
AlwI GGATC 2 cut(s) 317, 343
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 3 cut(s) 5, 123, 515
AspLEI GCGC 2 cut(s) 170, 264
BanI GGYRCC 2 cut(s) 167, 299
BanII GRGCYC 1 cut(s) 201
BauI CACGAG 1 cut(s) 53
BbvI GCAGC 3 cut(s) 17, 135, 527
BccI CCATC 2 cut(s) 188, 358
BceAI ACGGC 2 cut(s) 156, 381
BciT130I CCWGG 2 cut(s) 128, 279
BcoDI GTCTC 1 cut(s) 562
BfaI CTAG 1 cut(s) 583
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 3 cut(s) 6, 124, 516
BlsI GCNGC 3 cut(s) 7, 125, 517
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 128, 279
BmiI GGNNCC 6 cut(s) 76, 85, 169, 276, 283, 301
BmrFI CCNGG 2 cut(s) 128, 279
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 106, 527
BmuI ACTGGG 1 cut(s) 321
BpmI CTGGAG 4 cut(s) 119, 390, 465, 474
BsaHI GRCGYC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 144
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 332, 452
Bse1I ACTGG 3 cut(s) 187, 327, 482
Bse3DI GCAATG 1 cut(s) 463
BseBI CCWGG 2 cut(s) 128, 279
BseDI CCNNGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 518
BseLI CCNNNNNNNGG 3 cut(s) 265, 332, 452
BseMI GCAATG 1 cut(s) 463
BseNI ACTGG 3 cut(s) 187, 327, 482
BseRI GAGGAG 3 cut(s) 26, 29, 124
BseXI GCAGC 3 cut(s) 17, 135, 527
Bsh1236I CGCG 1 cut(s) 233
BshFI GGCC 1 cut(s) 143
BshNI GGYRCC 2 cut(s) 167, 299
BsiSI CCGG 2 cut(s) 165, 297
BsiWI CGTACG 1 cut(s) 237
BslFI GGGAC 2 cut(s) 388, 482
BslI CCNNNNNNNGG 3 cut(s) 265, 332, 452
BsmAI GTCTC 1 cut(s) 562
BsmFI GGGAC 2 cut(s) 388, 482
BsnI GGCC 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 2 cut(s) 322, 335
BspACI CCGC 1 cut(s) 303
BspANI GGCC 1 cut(s) 143
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 6 cut(s) 76, 85, 169, 276, 283, 301
BspPI GGATC 2 cut(s) 317, 343
BspT107I GGYRCC 2 cut(s) 167, 299
BsrDI GCAATG 1 cut(s) 463
BsrI ACTGG 3 cut(s) 187, 327, 482
BssECI CCNNGG 1 cut(s) 144
BssMI GATC 2 cut(s) 322, 335
BssNAI GTATAC 1 cut(s) 346
BssNI GRCGYC 1 cut(s) 168
BssSI CACGAG 1 cut(s) 53
BssT1I CCWWGG 1 cut(s) 144
Bst1107I GTATAC 1 cut(s) 346
Bst2BI CACGAG 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 128, 279
Bst4CI ACNGT 2 cut(s) 251, 310
BstACI GRCGYC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 121
BstF5I GGATG 1 cut(s) 518
BstFNI CGCG 1 cut(s) 233
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 2 cut(s) 170, 264
BstKTI GATC 2 cut(s) 325, 338
BstMAI GTCTC 1 cut(s) 562
BstMBI GATC 2 cut(s) 322, 335
BstMWI GCNNNNNNNGC 5 cut(s) 83, 120, 167, 239, 311
BstNI CCWGG 2 cut(s) 128, 279
BstNSI RCATGY 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 126, 277
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 3 cut(s) 17, 135, 527
BstX2I RGATCY 1 cut(s) 335
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 1 cut(s) 335
BstZ17I GTATAC 1 cut(s) 346
BsuRI GGCC 1 cut(s) 143
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 518
Cac8I GCNNGC 1 cut(s) 121
CfoI GCGC 2 cut(s) 170, 264
CseI GACGC 1 cut(s) 222
Csp6I GTAC 2 cut(s) 238, 381
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviAII CATG 2 cut(s) 156, 532
CviQI GTAC 2 cut(s) 238, 381
DinI GGCGCC 1 cut(s) 169
DpnI GATC 2 cut(s) 324, 337
DpnII GATC 2 cut(s) 322, 335
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 201
EcoRII CCWGG 2 cut(s) 126, 277
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 201
EgeI GGCGCC 1 cut(s) 169
EheI GGCGCC 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 2 cut(s) 159, 535
FaiI YATR 6 cut(s) 153, 157, 272, 333, 346, 533
FaqI GGGAC 2 cut(s) 388, 482
FatI CATG 2 cut(s) 155, 531
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 3 cut(s) 6, 124, 516
FokI GGATG 1 cut(s) 505
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 3 cut(s) 6, 124, 516
FspBI CTAG 1 cut(s) 583
GlaI GCGC 2 cut(s) 169, 263
GluI GCNGC 3 cut(s) 6, 124, 516
GsuI CTGGAG 4 cut(s) 119, 390, 465, 474
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 143
HapII CCGG 2 cut(s) 165, 297
HgaI GACGC 1 cut(s) 222
HhaI GCGC 2 cut(s) 170, 264
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 2 cut(s) 159, 535
Hin6I GCGC 2 cut(s) 168, 262
HinP1I GCGC 2 cut(s) 168, 262
HinfI GANTC 4 cut(s) 442, 474, 506, 550
HpaII CCGG 2 cut(s) 165, 297
Hpy166II GTNNAC 2 cut(s) 346, 428
Hpy188I TCNGA 4 cut(s) 74, 193, 402, 447
Hpy188III TCNNGA 2 cut(s) 136, 547
Hpy8I GTNNAC 2 cut(s) 346, 428
Hpy99I CGWCG 2 cut(s) 238, 506
HpyAV CCTTC 2 cut(s) 74, 460
HpyCH4III ACNGT 2 cut(s) 251, 310
HpyCH4V TGCA 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 120, 167, 239, 311
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 2 cut(s) 159, 535
HspAI GCGC 2 cut(s) 168, 262
KasI GGCGCC 1 cut(s) 167
Kzo9I GATC 2 cut(s) 322, 335
LmnI GCTCC 6 cut(s) 74, 83, 111, 280, 287, 540
Lsp1109I GCAGC 3 cut(s) 17, 135, 527
LweI GCATC 2 cut(s) 106, 527
MaeI CTAG 1 cut(s) 583
MaeIII GTNAC 4 cut(s) 203, 251, 287, 484
MalI GATC 2 cut(s) 324, 337
MboI GATC 2 cut(s) 322, 335
MboII GAAGA 4 cut(s) 19, 22, 345, 571
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 201
MluI ACGCGT 1 cut(s) 231
Mly113I GGCGCC 1 cut(s) 168
MmeI TCCRAC 2 cut(s) 425, 470
MnlI CCTC 9 cut(s) 30, 44, 47, 50, 62, 102, 172, 218, 365
Mph1103I ATGCAT 1 cut(s) 157
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 2 cut(s) 165, 297
MspR9I CCNGG 2 cut(s) 128, 279
MvaI CCWGG 2 cut(s) 128, 279
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 5 cut(s) 83, 120, 167, 239, 311
NarI GGCGCC 1 cut(s) 168
NdeII GATC 2 cut(s) 322, 335
NlaIII CATG 2 cut(s) 159, 535
NlaIV GGNNCC 6 cut(s) 76, 85, 169, 276, 283, 301
NmuCI GTSAC 1 cut(s) 287
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PfeI GAWTC 4 cut(s) 442, 474, 506, 550
Pfl23II CGTACG 1 cut(s) 237
PkrI GCNGC 3 cut(s) 7, 125, 517
PluTI GGCGCC 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 126, 277
PspGI CCWGG 2 cut(s) 126, 277
PspLI CGTACG 1 cut(s) 237
PspN4I GGNNCC 6 cut(s) 76, 85, 169, 276, 283, 301
PsuI RGATCY 1 cut(s) 335
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 239, 382
RsaNI GTAC 2 cut(s) 238, 381
SatI GCNGC 3 cut(s) 6, 124, 516
Sau3AI GATC 2 cut(s) 322, 335
ScaI AGTACT 1 cut(s) 382
ScrFI CCNGG 2 cut(s) 128, 279
SduI GDGCHC 1 cut(s) 201
SetI ASST 5 cut(s) 22, 210, 229, 452, 496
SfaNI GCATC 2 cut(s) 106, 527
SfoI GGCGCC 1 cut(s) 169
SsiI CCGC 1 cut(s) 303
SspDI GGCGCC 1 cut(s) 167
SspMI CTAG 1 cut(s) 583
StyD4I CCNGG 2 cut(s) 126, 277
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 2 cut(s) 251, 310
TaqII GACCGA 1 cut(s) 335
TatI WGTACW 1 cut(s) 380
TfiI GAWTC 4 cut(s) 442, 474, 506, 550
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 3 cut(s) 5, 123, 515
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 1 cut(s) 520
XceI RCATGY 1 cut(s) 159
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 1 cut(s) 583
ZrmI AGTACT 1 cut(s) 382
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.