FvH4_4g19970

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015336
Physical Location & Seq
Forward (+)
23417850 .. 23418311
462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19970.t1

Sequence Viewer

Length: 462 bp
ATGTGTCCCACCGGCCGGACCCTGACATCTTCGTCCTCGTTCTCCGACCTCCGCCCCGCATTCGACATCCTCGACGCCGACCGCGACGGCAAGATCAGCAAGGACGACCTCCGCGCCTTCTACGTCGGATTCTCCTCCTCCGGCGCCGACGAGAGCCTCATCCGCGCCATGATGTCCGCTGCCGACTCCAACTCCGACGGCTTCGTCGAGTACCACGAGTTCGAGCGCGTCCTCGCCTGTCAGAACCCCTCCGGCGTCATGGAGGACGTGTTCAAGGTCATGGACCGCGACGGCGACGGCAAGCTCAGCCACGAAGACTTGAAAAGCTACATGAACTTGGCCGGGTTTCCGGCCAGCGACGACGACATTGCGGGGATGATCAAATTGGGCGGCGGAGATGAAGCTCAAGGCGTGGACTACGCCGGGTTGCTGAAGATCCTGGCCGTTGATCAAGCCAGCTAG

Protein Analysis

154

Amino Acids

16.41

Weight (kDa)

4.28

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 14 - 77 1e-12 EF-hand domain pair
EF-hand_5 PF13202 19 - 40 1.7e-06 EF hand
EF-hand_7 PF13499 87 - 128 4.5e-06 EF-hand domain pair
EF-hand_5 PF13202 87 - 109 5.4e-06 EF hand
EF-hand_6 PF13405 87 - 113 5e-06 EF-hand domain
EF-hand_1 PF00036 88 - 112 3.6e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49480
fragaria_vesca FvH4_4g19970
malus_domestica MD04G1036600.v1.1 MD13G1151300.v1.1 MD16G1152300.v1.1
prunus_persica Prupe.1G115600_v2.0.a1
pyrus_communis pycom16g12960
rosa_chinensis RchiOBHm_Chr4g0425671
rosa_laevigata RLG00000007348
rosa_multiflora Rmu_co8455781.1_g000002
rosa_rugosa Rorug04G0204700
rosa_samantha Rh4AG262000 Rh4BG268100 Rh4CG279200 Rh4DG263800
rosa_wichuraiana Rw4G022640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 31, 203
AccB1I GGYRCC 1 cut(s) 143
AccII CGCG 5 cut(s) 84, 114, 165, 228, 288
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 4 cut(s) 13, 339, 351, 441
AcuI CTGAAG 1 cut(s) 452
AcyI GRCGYC 3 cut(s) 75, 144, 255
AfaI GTAC 1 cut(s) 212
AfiI CCNNNNNNNGG 1 cut(s) 15
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 2 cut(s) 274, 322
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 1 cut(s) 438
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
AluBI AGCT 4 cut(s) 304, 327, 404, 459
AluI AGCT 4 cut(s) 304, 327, 404, 459
AlwI GGATC 1 cut(s) 430
AoxI GGCC 4 cut(s) 13, 339, 351, 441
ApeKI GCWGC 1 cut(s) 179
AspLEI GCGC 4 cut(s) 116, 146, 167, 228
AspS9I GGNCC 2 cut(s) 18, 283
AsuC2I CCSGG 2 cut(s) 343, 424
AvaII GGWCC 2 cut(s) 18, 283
BanI GGYRCC 1 cut(s) 143
BauI CACGAG 1 cut(s) 215
BbsI GAAGAC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 166
BceAI ACGGC 5 cut(s) 103, 214, 307, 313, 428
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 1 cut(s) 440
BclI TGATCA 2 cut(s) 378, 448
BcnI CCSGG 2 cut(s) 343, 424
BfaI CTAG 1 cut(s) 460
BfoI RGCGCY 1 cut(s) 147
BisI GCNGC 2 cut(s) 180, 391
BlpI GCTNAGC 1 cut(s) 305
BlsI GCNGC 2 cut(s) 181, 392
Bme1390I CCNGG 3 cut(s) 343, 424, 440
Bme18I GGWCC 2 cut(s) 18, 283
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 18, 283
BmiI GGNNCC 2 cut(s) 20, 145
BmrFI CCNGG 3 cut(s) 343, 424, 440
BpiI GAAGAC 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 390
BpuMI CCSGG 2 cut(s) 343, 424
BsaHI GRCGYC 3 cut(s) 75, 144, 255
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 254, 284
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 366
BseBI CCWGG 1 cut(s) 440
BseGI GGATG 3 cut(s) 66, 159, 381
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 319
BseRI GAGGAG 2 cut(s) 124, 127
BseX3I CGGCCG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 166
Bsh1236I CGCG 5 cut(s) 84, 114, 165, 228, 288
Bsh1285I CGRYCG 2 cut(s) 16, 82
BshFI GGCC 4 cut(s) 15, 341, 353, 443
BshNI GGYRCC 1 cut(s) 143
BsiEI CGRYCG 2 cut(s) 16, 82
BsiSI CCGG 7 cut(s) 12, 16, 141, 252, 342, 350, 423
BslI CCNNNNNNNGG 1 cut(s) 15
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 4 cut(s) 15, 341, 353, 443
Bsp143I GATC 4 cut(s) 93, 378, 435, 448
Bsp1720I GCTNAGC 1 cut(s) 305
BspANI GGCC 4 cut(s) 15, 341, 353, 443
BspCNI CTCAG 1 cut(s) 318
BspFNI CGCG 5 cut(s) 84, 114, 165, 228, 288
BspLI GGNNCC 2 cut(s) 20, 145
BspPI GGATC 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 11
BssMI GATC 4 cut(s) 93, 378, 435, 448
BssNI GRCGYC 3 cut(s) 75, 144, 255
BssSI CACGAG 1 cut(s) 215
Bst2BI CACGAG 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 440
BstACI GRCGYC 3 cut(s) 75, 144, 255
BstC8I GCNNGC 3 cut(s) 302, 355, 457
BstDEI CTNAG 1 cut(s) 305
BstF5I GGATG 3 cut(s) 66, 159, 381
BstFNI CGCG 5 cut(s) 84, 114, 165, 228, 288
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 4 cut(s) 116, 146, 167, 228
BstKTI GATC 4 cut(s) 96, 381, 438, 451
BstMBI GATC 4 cut(s) 93, 378, 435, 448
BstMCI CGRYCG 2 cut(s) 16, 82
BstMWI GCNNNNNNNGC 3 cut(s) 96, 162, 306
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 3 cut(s) 341, 422, 438
BstUI CGCG 5 cut(s) 84, 114, 165, 228, 288
BstV1I GCAGC 1 cut(s) 166
BstV2I GAAGAC 1 cut(s) 321
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
BstZI CGGCCG 1 cut(s) 13
BsuRI GGCC 4 cut(s) 15, 341, 353, 443
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 3 cut(s) 66, 159, 381
Cac8I GCNNGC 3 cut(s) 302, 355, 457
CfoI GCGC 4 cut(s) 116, 146, 167, 228
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 18, 283
CseI GACGC 3 cut(s) 83, 217, 244
Csp6I GTAC 1 cut(s) 211
CviAII CATG 4 cut(s) 169, 259, 280, 331
CviQI GTAC 1 cut(s) 211
DdeI CTNAG 1 cut(s) 305
DinI GGCGCC 1 cut(s) 145
DpnI GATC 4 cut(s) 95, 380, 437, 450
DpnII GATC 4 cut(s) 93, 378, 435, 448
DrdI GACNNNNNNGTC 2 cut(s) 31, 203
DseDI GACNNNNNNGTC 2 cut(s) 31, 203
EaeI YGGCCR 4 cut(s) 13, 339, 351, 441
EagI CGGCCG 1 cut(s) 13
EciI GGCGGA 2 cut(s) 41, 408
EclXI CGGCCG 1 cut(s) 13
Eco47I GGWCC 2 cut(s) 18, 283
Eco52I CGGCCG 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 438
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
FaeI CATG 4 cut(s) 172, 262, 283, 334
FaiI YATR 4 cut(s) 170, 260, 281, 332
FatI CATG 4 cut(s) 168, 258, 279, 330
FauI CCCGC 2 cut(s) 64, 364
FbaI TGATCA 2 cut(s) 378, 448
Fnu4HI GCNGC 2 cut(s) 180, 391
FokI GGATG 3 cut(s) 53, 146, 388
Fsp4HI GCNGC 2 cut(s) 180, 391
FspBI CTAG 1 cut(s) 460
GlaI GCGC 4 cut(s) 115, 145, 166, 227
GluI GCNGC 2 cut(s) 180, 391
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 4 cut(s) 15, 341, 353, 443
HapII CCGG 7 cut(s) 12, 16, 141, 252, 342, 350, 423
HgaI GACGC 3 cut(s) 83, 217, 244
HhaI GCGC 4 cut(s) 116, 146, 167, 228
Hin1I GRCGYC 3 cut(s) 75, 144, 255
Hin1II CATG 4 cut(s) 172, 262, 283, 334
Hin6I GCGC 4 cut(s) 114, 144, 165, 226
HinP1I GCGC 4 cut(s) 114, 144, 165, 226
HinfI GANTC 2 cut(s) 129, 185
HpaII CCGG 7 cut(s) 12, 16, 141, 252, 342, 350, 423
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 4 cut(s) 46, 128, 196, 243
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 1 cut(s) 127
HpyCH4IV ACGT 2 cut(s) 123, 267
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 162, 306
HpyF3I CTNAG 1 cut(s) 305
HpySE526I ACGT 2 cut(s) 123, 267
Hsp92I GRCGYC 3 cut(s) 75, 144, 255
Hsp92II CATG 4 cut(s) 172, 262, 283, 334
HspAI GCGC 4 cut(s) 114, 144, 165, 226
KasI GGCGCC 1 cut(s) 143
Ksp22I TGATCA 2 cut(s) 378, 448
Kzo9I GATC 4 cut(s) 93, 378, 435, 448
Lsp1109I GCAGC 1 cut(s) 166
MaeI CTAG 1 cut(s) 460
MaeII ACGT 2 cut(s) 123, 267
MalI GATC 4 cut(s) 95, 380, 437, 450
MboI GATC 4 cut(s) 93, 378, 435, 448
MboII GAAGA 3 cut(s) 21, 326, 445
MflI RGATCY 1 cut(s) 435
MluCI AATT 1 cut(s) 383
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 4 cut(s) 69, 106, 213, 219
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 7 cut(s) 12, 16, 141, 252, 342, 350, 423
MspR9I CCNGG 3 cut(s) 343, 424, 440
Mva1269I GAATGC 1 cut(s) 59
MvaI CCWGG 1 cut(s) 440
MvnI CGCG 5 cut(s) 84, 114, 165, 228, 288
MwoI GCNNNNNNNGC 3 cut(s) 96, 162, 306
NarI GGCGCC 1 cut(s) 144
NciI CCSGG 2 cut(s) 343, 424
NdeII GATC 4 cut(s) 93, 378, 435, 448
NlaIII CATG 4 cut(s) 172, 262, 283, 334
NlaIV GGNNCC 2 cut(s) 20, 145
PcsI WCGNNNNNNNCGW 4 cut(s) 69, 81, 204, 213
PctI GAATGC 1 cut(s) 59
PfeI GAWTC 1 cut(s) 129
PkrI GCNGC 2 cut(s) 181, 392
PleI GAGTC 1 cut(s) 179
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 179
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 2 cut(s) 20, 145
PspPI GGNCC 2 cut(s) 18, 283
PsrI GAACNNNNNNTAC 2 cut(s) 203, 235
PsuI RGATCY 1 cut(s) 435
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
SatI GCNGC 2 cut(s) 180, 391
Sau3AI GATC 4 cut(s) 93, 378, 435, 448
Sau96I GGNCC 2 cut(s) 18, 283
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 3 cut(s) 343, 424, 440
SetI ASST 9 cut(s) 51, 111, 126, 270, 279, 306, 329, 406, 461
SfoI GGCGCC 1 cut(s) 145
SinI GGWCC 2 cut(s) 18, 283
SmlI CTYRAG 1 cut(s) 405
SmoI CTYRAG 1 cut(s) 405
Sse9I AATT 1 cut(s) 383
SspDI GGCGCC 1 cut(s) 143
SspMI CTAG 1 cut(s) 460
StyD4I CCNGG 3 cut(s) 341, 422, 438
TaiI ACGT 2 cut(s) 126, 270
TaqI TCGA 4 cut(s) 63, 72, 207, 222
TasI AATT 1 cut(s) 383
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 1 cut(s) 129
TseI GCWGC 1 cut(s) 179
TspDTI ATGAA 2 cut(s) 347, 414
VpaK11BI GGWCC 2 cut(s) 18, 283
XspI CTAG 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.