Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0015336

Physical Location & Seq

Genomic Coordinates Forward (+)

4098127 .. 4098438

Gene Structure 312 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1036600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 312 bp

ATGATGTCTGCCGCTGATTCCGACAACGACGGGTTCGTCCAGTATGAGGAGTTCGAGCGCGTCTTGGCTTGCGGCGACAGGGCAAAAAGCTCGGGAGTGATGGATGACGTGTTTAAGGTGATGGACAAGGACGACGACGGGAAACTCAGCCACGAGGACATGAAGAATTACATGAGCAGCGCCGGCTTCGTCGTCACCGATGAAGATATCAAGGCCATGATTAAACTAGGCGGCGGCGATGAGAACCAAGGCGTGACGTATGCTGGCTTGCTTAAGATCCTGGCCGTTGATCAAGTCAGCGAAGCGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0009628 GO:0009651 GO:0042538 GO:0043167 GO:0043169 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko04014 ko04015 ko04016 ko04020 ko04022 ko04024 ko04070 ko04114 ko04218 ko04261 ko04270 ko04371 ko04626 ko04713 ko04720 ko04722 ko04728 ko04740 ko04744 ko04745 ko04750 ko04910 ko04912 ko04915 ko04916 ko04921 ko04922 ko04924 ko04925 ko04970 ko04971 ko05010 ko05031 ko05034 ko05133 ko05152 ko05167 ko05200 ko05214 ko05418 map04014 map04015 map04016 map04020 map04022 map04024 map04070 map04114 map04218 map04261 map04270 map04371 map04626 map04713 map04720 map04722 map04728 map04740 map04744 map04745 map04750 map04910 map04912 map04915 map04916 map04921 map04922 map04924 map04925 map04970 map04971 map05010 map05031 map05034 map05133 map05152 map05167 map05200 map05214 map05418

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

104

Amino Acids

11.16

Weight (kDa)

4.19

Isoelectric Point (pI)

31.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	3 - 59	2e-09	EF-hand domain pair
EF-hand_6	PF13405	35 - 62	7.9e-06	EF-hand domain
EF-hand_7	PF13499	36 - 91	5.6e-07	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49480
fragaria_vesca	FvH4_4g19970
malus_domestica	MD04G1036600.v1.1 MD13G1151300.v1.1 MD16G1152300.v1.1
prunus_persica	Prupe.1G115600_v2.0.a1
pyrus_communis	pycom16g12960
rosa_chinensis	RchiOBHm_Chr4g0425671
rosa_laevigata	RLG00000007348
rosa_multiflora	Rmu_co8455781.1_g000002
rosa_rugosa	Rorug04G0204700
rosa_samantha	Rh4AG262000 Rh4BG268100 Rh4CG279200 Rh4DG263800
rosa_wichuraiana	Rw4G022640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	35
AccII	CGCG	1 cut(s)	60
AciI	CCGC	4 cut(s)	12, 72, 231, 234
AclWI	GGATC	1 cut(s)	271
AcoI	YGGCCR	1 cut(s)	282
AfiI	CCNNNNNNNGG	1 cut(s)	46
AflII	CTTAAG	1 cut(s)	272
AflIII	ACRYGT	1 cut(s)	108
AjiI	CACGTC	1 cut(s)	109
AjnI	CCWGG	1 cut(s)	279
AluBI	AGCT	1 cut(s)	90
AluI	AGCT	1 cut(s)	90
AlwI	GGATC	1 cut(s)	271
Ama87I	CYCGRG	1 cut(s)	91
AoxI	GGCC	2 cut(s)	213, 282
ApeKI	GCWGC	1 cut(s)	177
AspLEI	GCGC	2 cut(s)	60, 182
AsuHPI	GGTGA	2 cut(s)	130, 187
AvaI	CYCGRG	1 cut(s)	91
BauI	CACGAG	1 cut(s)	152
BbvI	GCAGC	1 cut(s)	189
BccI	CCATC	2 cut(s)	94, 115
BceAI	ACGGC	1 cut(s)	269
BcgI	CGANNNNNNTGC	2 cut(s)	72, 106
BciT130I	CCWGG	1 cut(s)	281
BclI	TGATCA	1 cut(s)	289
BfaI	CTAG	1 cut(s)	227
BfoI	RGCGCY	1 cut(s)	183
BfrI	CTTAAG	1 cut(s)	272
BisI	GCNGC	5 cut(s)	12, 73, 178, 232, 235
BlsI	GCNGC	5 cut(s)	13, 74, 179, 233, 236
Bme1390I	CCNGG	1 cut(s)	281
BmeT110I	CYCGRG	1 cut(s)	91
BmgBI	CACGTC	1 cut(s)	109
BmrFI	CCNGG	1 cut(s)	281
BsaJI	CCNNGG	1 cut(s)	247
Bsc4I	CCNNNNNNNGG	1 cut(s)	46
Bse118I	RCCGGY	1 cut(s)	182
Bse1I	ACTGG	1 cut(s)	40
BseBI	CCWGG	1 cut(s)	281
BseDI	CCNNGG	1 cut(s)	247
BseGI	GGATG	1 cut(s)	109
BseLI	CCNNNNNNNGG	1 cut(s)	46
BseMII	CTCAG	1 cut(s)	160
BseNI	ACTGG	1 cut(s)	40
BseRI	GAGGAG	1 cut(s)	62
BseXI	GCAGC	1 cut(s)	189
Bsh1236I	CGCG	1 cut(s)	60
BshFI	GGCC	2 cut(s)	215, 284
BsiHKCI	CYCGRG	1 cut(s)	91
BsiSI	CCGG	1 cut(s)	183
BslI	CCNNNNNNNGG	1 cut(s)	46
BsnI	GGCC	2 cut(s)	215, 284
BsoBI	CYCGRG	1 cut(s)	91
Bsp143I	GATC	2 cut(s)	276, 289
BspACI	CCGC	4 cut(s)	12, 72, 231, 234
BspANI	GGCC	2 cut(s)	215, 284
BspCNI	CTCAG	1 cut(s)	159
BspFNI	CGCG	1 cut(s)	60
BspPI	GGATC	1 cut(s)	271
BspTI	CTTAAG	1 cut(s)	272
BsrFI	RCCGGY	1 cut(s)	182
BsrI	ACTGG	1 cut(s)	40
BssAI	RCCGGY	1 cut(s)	182
BssECI	CCNNGG	1 cut(s)	247
BssMI	GATC	2 cut(s)	276, 289
BssSI	CACGAG	1 cut(s)	152
BssT1I	CCWWGG	1 cut(s)	247
Bst2BI	CACGAG	1 cut(s)	152
Bst2UI	CCWGG	1 cut(s)	281
BstAFI	CTTAAG	1 cut(s)	272
BstC8I	GCNNGC	4 cut(s)	70, 184, 265, 269
BstDEI	CTNAG	1 cut(s)	146
BstF5I	GGATG	1 cut(s)	109
BstFNI	CGCG	1 cut(s)	60
BstH2I	RGCGCY	1 cut(s)	183
BstHHI	GCGC	2 cut(s)	60, 182
BstKTI	GATC	2 cut(s)	279, 292
BstMBI	GATC	2 cut(s)	276, 289
BstMWI	GCNNNNNNNGC	1 cut(s)	183
BstNI	CCWGG	1 cut(s)	281
BstSCI	CCNGG	1 cut(s)	279
BstUI	CGCG	1 cut(s)	60
BstV1I	GCAGC	1 cut(s)	189
BstX2I	RGATCY	1 cut(s)	276
BstYI	RGATCY	1 cut(s)	276
BsuRI	GGCC	2 cut(s)	215, 284
BtgZI	GCGATG	1 cut(s)	252
BtrI	CACGTC	1 cut(s)	109
BtsCI	GGATG	1 cut(s)	109
Cac8I	GCNNGC	4 cut(s)	70, 184, 265, 269
CfoI	GCGC	2 cut(s)	60, 182
Cfr10I	RCCGGY	1 cut(s)	182
CseI	GACGC	2 cut(s)	49, 294
CviAII	CATG	3 cut(s)	160, 172, 217
CviJI	RGCY	7 cut(s)	68, 90, 150, 186, 215, 267, 284
CviKI_1	RGCY	7 cut(s)	68, 90, 150, 186, 215, 267, 284
DdeI	CTNAG	1 cut(s)	146
DpnI	GATC	2 cut(s)	278, 291
DpnII	GATC	2 cut(s)	276, 289
DrdI	GACNNNNNNGTC	1 cut(s)	35
DseDI	GACNNNNNNGTC	1 cut(s)	35
EaeI	YGGCCR	1 cut(s)	282
Eco130I	CCWWGG	1 cut(s)	247
Eco32I	GATATC	1 cut(s)	208
Eco88I	CYCGRG	1 cut(s)	91
EcoRII	CCWGG	1 cut(s)	279
EcoRV	GATATC	1 cut(s)	208
EcoT14I	CCWWGG	1 cut(s)	247
ErhI	CCWWGG	1 cut(s)	247
FaeI	CATG	3 cut(s)	163, 175, 220
FaiI	YATR	5 cut(s)	45, 161, 173, 218, 261
FatI	CATG	3 cut(s)	159, 171, 216
FbaI	TGATCA	1 cut(s)	289
Fnu4HI	GCNGC	5 cut(s)	12, 73, 178, 232, 235
FokI	GGATG	1 cut(s)	116
Fsp4HI	GCNGC	5 cut(s)	12, 73, 178, 232, 235
FspBI	CTAG	1 cut(s)	227
GlaI	GCGC	2 cut(s)	59, 181
GluI	GCNGC	5 cut(s)	12, 73, 178, 232, 235
HaeII	RGCGCY	1 cut(s)	183
HaeIII	GGCC	2 cut(s)	215, 284
HapII	CCGG	1 cut(s)	183
HgaI	GACGC	2 cut(s)	49, 294
HhaI	GCGC	2 cut(s)	60, 182
Hin1II	CATG	3 cut(s)	163, 175, 220
Hin6I	GCGC	2 cut(s)	58, 180
HinP1I	GCGC	2 cut(s)	58, 180
HinfI	GANTC	1 cut(s)	17
HpaII	CCGG	1 cut(s)	183
HphI	GGTGA	2 cut(s)	130, 187
Hpy188I	TCNGA	1 cut(s)	22
Hpy188III	TCNNGA	2 cut(s)	93, 309
Hpy99I	CGWCG	4 cut(s)	32, 137, 140, 194
HpyCH4IV	ACGT	2 cut(s)	108, 257
HpyF10VI	GCNNNNNNNGC	1 cut(s)	183
HpyF3I	CTNAG	1 cut(s)	146
HpySE526I	ACGT	2 cut(s)	108, 257
Hsp92II	CATG	3 cut(s)	163, 175, 220
HspAI	GCGC	2 cut(s)	58, 180
KroI	GCCGGC	1 cut(s)	182
KroNI	GCCGGC	1 cut(s)	184
Ksp22I	TGATCA	1 cut(s)	289
Kzo9I	GATC	2 cut(s)	276, 289
LpnPI	CCDG	6 cut(s)	53, 64, 196, 249, 266, 293
Lsp1109I	GCAGC	1 cut(s)	189
MaeI	CTAG	1 cut(s)	227
MaeII	ACGT	2 cut(s)	108, 257
MaeIII	GTNAC	2 cut(s)	193, 253
MalI	GATC	2 cut(s)	278, 291
MboI	GATC	2 cut(s)	276, 289
MboII	GAAGA	2 cut(s)	175, 215
MflI	RGATCY	1 cut(s)	276
MluCI	AATT	1 cut(s)	166
MmeI	TCCRAC	1 cut(s)	45
MnlI	CCTC	2 cut(s)	40, 148
MroNI	GCCGGC	1 cut(s)	182
MseI	TTAA	3 cut(s)	114, 222, 273
MspA1I	CMGCKG	1 cut(s)	14
MspCI	CTTAAG	1 cut(s)	272
MspI	CCGG	1 cut(s)	183
MspR9I	CCNGG	1 cut(s)	281
MvaI	CCWGG	1 cut(s)	281
MvnI	CGCG	1 cut(s)	60
MwoI	GCNNNNNNNGC	1 cut(s)	183
NaeI	GCCGGC	1 cut(s)	184
NdeII	GATC	2 cut(s)	276, 289
NgoMIV	GCCGGC	1 cut(s)	182
NlaIII	CATG	3 cut(s)	163, 175, 220
NmuCI	GTSAC	2 cut(s)	193, 253
PcsI	WCGNNNNNNNCGW	2 cut(s)	33, 195
PdiI	GCCGGC	1 cut(s)	184
PfeI	GAWTC	1 cut(s)	17
PkrI	GCNGC	5 cut(s)	13, 74, 179, 233, 236
Psp6I	CCWGG	1 cut(s)	279
PspGI	CCWGG	1 cut(s)	279
PsrI	GAACNNNNNNTAC	2 cut(s)	35, 67
PsuI	RGATCY	1 cut(s)	276
SaqAI	TTAA	3 cut(s)	114, 222, 273
SatI	GCNGC	5 cut(s)	12, 73, 178, 232, 235
Sau3AI	GATC	2 cut(s)	276, 289
ScrFI	CCNGG	1 cut(s)	281
SetI	ASST	4 cut(s)	92, 111, 120, 260
SmlI	CTYRAG	1 cut(s)	272
SmoI	CTYRAG	1 cut(s)	272
Sse9I	AATT	1 cut(s)	166
SsiI	CCGC	4 cut(s)	12, 72, 231, 234
SspMI	CTAG	1 cut(s)	227
StyD4I	CCNGG	1 cut(s)	279
StyI	CCWWGG	1 cut(s)	247
TaiI	ACGT	2 cut(s)	111, 260
TaqI	TCGA	1 cut(s)	54
TasI	AATT	1 cut(s)	166
TauI	GCSGC	4 cut(s)	14, 75, 234, 237
TfiI	GAWTC	1 cut(s)	17
Tru1I	TTAA	3 cut(s)	114, 222, 273
Tru9I	TTAA	3 cut(s)	114, 222, 273
TseFI	GTSAC	2 cut(s)	193, 253
TseI	GCWGC	1 cut(s)	177
Tsp45I	GTSAC	2 cut(s)	193, 253
TspDTI	ATGAA	2 cut(s)	176, 216
Vha464I	CTTAAG	1 cut(s)	272
XspI	CTAG	1 cut(s)	227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.