Rh4AG262000

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0015336
Physical Location & Seq
Forward (+)
57784795 .. 57787794
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG262000.1

Sequence Viewer

Length: 486 bp
ATGTGTCCCACCGGCCGAACCCTAACGTCGACGACGTTGACCTCTTCGTCCTCGTCCTCCGACCTCCGCCCCGCATTCGACGTCCTCGACGCCGACCGCGACGGCAAGATCAGCAAGGACGATCTCCGCACCTTCTACGCCGGATTCTCCAGCTCCGGCGCCGACGAGAGCCTCATCCGGGCGATGATGTCCGCCGCCGACTCCAACTCCGACGGCTTCGTCGAGTACGATGAGTTCGAGCGCGTGCTCGCCTGTCAGAAACCCTCGGCCACTGGCGGCGTCATGGAGGACGTGTTCAAGGTTATGGACCGCGACGGCGACGGCAGGCTTAGCCACGAAGATTTAAAGAGCTACATGAACTTGGCTGGGTTTCCGGCCACCGACGACGACATTGCGGCCATGATCAGATTGGGCGGCGGCGATGAAACTCAAGGCGTCGACTACGCCGGGTTGCTGAAGATCCTGGCCGTTGATCAAGTCAGCTAG

Protein Analysis

161

Amino Acids

17.2

Weight (kDa)

4.25

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 20 - 82 2.4e-13 EF-hand domain pair
EF-hand_5 PF13202 24 - 45 9.6e-07 EF hand
EF-hand_7 PF13499 95 - 137 1.5e-06 EF-hand domain pair
EF-hand_5 PF13202 95 - 117 1e-05 EF hand
EF-hand_6 PF13405 95 - 121 8.1e-06 EF-hand domain
EF-hand_1 PF00036 96 - 120 1e-05 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49480
fragaria_vesca FvH4_4g19970
malus_domestica MD04G1036600.v1.1 MD13G1151300.v1.1 MD16G1152300.v1.1
prunus_persica Prupe.1G115600_v2.0.a1
pyrus_communis pycom16g12960
rosa_chinensis RchiOBHm_Chr4g0425671
rosa_laevigata RLG00000007348
rosa_multiflora Rmu_co8455781.1_g000002
rosa_rugosa Rorug04G0204700
rosa_samantha Rh4AG262000 Rh4BG268100 Rh4CG279200 Rh4DG263800
rosa_wichuraiana Rw4G022640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 46, 218
AatII GACGTC 1 cut(s) 84
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 2 cut(s) 29, 438
AccII CGCG 3 cut(s) 99, 243, 312
AclWI GGATC 1 cut(s) 454
AcoI YGGCCR 5 cut(s) 13, 267, 375, 396, 465
AcuI CTGAAG 1 cut(s) 476
AcyI GRCGYC 5 cut(s) 81, 90, 159, 279, 435
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 178
AflIII ACRYGT 1 cut(s) 291
AgsI TTSAA 1 cut(s) 298
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 462
AloI GAACNNNNNNTCC 2 cut(s) 278, 310
AluBI AGCT 3 cut(s) 153, 351, 483
AluI AGCT 3 cut(s) 153, 351, 483
Alw21I GWGCWC 1 cut(s) 249
AlwI GGATC 1 cut(s) 454
AoxI GGCC 5 cut(s) 13, 267, 375, 396, 465
AspLEI GCGC 2 cut(s) 161, 243
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 2 cut(s) 179, 448
AvaII GGWCC 1 cut(s) 307
BanI GGYRCC 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 249
BceAI ACGGC 5 cut(s) 118, 229, 331, 337, 452
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 1 cut(s) 464
BclI TGATCA 2 cut(s) 402, 472
BcnI CCSGG 2 cut(s) 179, 448
BfaI CTAG 1 cut(s) 484
BfoI RGCGCY 1 cut(s) 162
BisI GCNGC 5 cut(s) 195, 277, 396, 415, 418
BlpI GCTNAGC 1 cut(s) 329
BlsI GCNGC 5 cut(s) 196, 278, 397, 416, 419
Bme1390I CCNGG 3 cut(s) 179, 448, 464
Bme18I GGWCC 1 cut(s) 307
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 3 cut(s) 179, 448, 464
BpmI CTGGAG 1 cut(s) 133
Bpu1102I GCTNAGC 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 414
BpuMI CCSGG 2 cut(s) 179, 448
BsaHI GRCGYC 5 cut(s) 81, 90, 159, 279, 435
BsaJI CCNNGG 1 cut(s) 264
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 278, 308
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 1 cut(s) 390
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 264
BseGI GGATG 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 1 cut(s) 390
BseNI ACTGG 1 cut(s) 277
BseX3I CGGCCG 1 cut(s) 13
BseYI CCCAGC 1 cut(s) 365
Bsh1236I CGCG 3 cut(s) 99, 243, 312
Bsh1285I CGRYCG 2 cut(s) 16, 97
BshFI GGCC 5 cut(s) 15, 269, 377, 398, 467
BshNI GGYRCC 1 cut(s) 158
BsiEI CGRYCG 2 cut(s) 16, 97
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 6 cut(s) 12, 141, 156, 178, 374, 447
BslI CCNNNNNNNGG 1 cut(s) 178
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 5 cut(s) 15, 269, 377, 398, 467
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 5 cut(s) 108, 121, 402, 459, 472
Bsp1720I GCTNAGC 1 cut(s) 329
BspANI GGCC 5 cut(s) 15, 269, 377, 398, 467
BspFNI CGCG 3 cut(s) 99, 243, 312
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 1 cut(s) 277
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 5 cut(s) 108, 121, 402, 459, 472
BssNI GRCGYC 5 cut(s) 81, 90, 159, 279, 435
Bst2UI CCWGG 1 cut(s) 464
Bst6I CTCTTC 1 cut(s) 49
BstACI GRCGYC 5 cut(s) 81, 90, 159, 279, 435
BstC8I GCNNGC 3 cut(s) 245, 249, 326
BstDEI CTNAG 1 cut(s) 329
BstF5I GGATG 1 cut(s) 174
BstFNI CGCG 3 cut(s) 99, 243, 312
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 2 cut(s) 161, 243
BstKTI GATC 5 cut(s) 111, 124, 405, 462, 475
BstMBI GATC 5 cut(s) 108, 121, 402, 459, 472
BstMCI CGRYCG 2 cut(s) 16, 97
BstMWI GCNNNNNNNGC 2 cut(s) 111, 330
BstNI CCWGG 1 cut(s) 464
BstSCI CCNGG 3 cut(s) 177, 446, 462
BstUI CGCG 3 cut(s) 99, 243, 312
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BstZI CGGCCG 1 cut(s) 13
BsuRI GGCC 5 cut(s) 15, 269, 377, 398, 467
BtgZI GCGATG 2 cut(s) 197, 435
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 270
Cac8I GCNNGC 3 cut(s) 245, 249, 326
CfoI GCGC 2 cut(s) 161, 243
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 307
CseI GACGC 3 cut(s) 98, 268, 424
Csp6I GTAC 1 cut(s) 226
CviAII CATG 3 cut(s) 283, 355, 400
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 329
DinI GGCGCC 1 cut(s) 160
DpnI GATC 5 cut(s) 110, 123, 404, 461, 474
DpnII GATC 5 cut(s) 108, 121, 402, 459, 472
DraI TTTAAA 1 cut(s) 345
DrdI GACNNNNNNGTC 2 cut(s) 46, 218
DseDI GACNNNNNNGTC 2 cut(s) 46, 218
EaeI YGGCCR 5 cut(s) 13, 267, 375, 396, 465
EagI CGGCCG 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
EciI GGCGGA 2 cut(s) 56, 181
EclXI CGGCCG 1 cut(s) 13
Eco47I GGWCC 1 cut(s) 307
Eco52I CGGCCG 1 cut(s) 13
Eco57I CTGAAG 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 462
EgeI GGCGCC 1 cut(s) 160
EheI GGCGCC 1 cut(s) 160
FaeI CATG 3 cut(s) 286, 358, 403
FaiI YATR 4 cut(s) 284, 305, 356, 401
FatI CATG 3 cut(s) 282, 354, 399
FauI CCCGC 1 cut(s) 79
FbaI TGATCA 2 cut(s) 402, 472
FblI GTMKAC 2 cut(s) 29, 438
Fnu4HI GCNGC 5 cut(s) 195, 277, 396, 415, 418
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 195, 277, 396, 415, 418
FspBI CTAG 1 cut(s) 484
GlaI GCGC 2 cut(s) 160, 242
GluI GCNGC 5 cut(s) 195, 277, 396, 415, 418
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 5 cut(s) 15, 269, 377, 398, 467
HapII CCGG 6 cut(s) 12, 141, 156, 178, 374, 447
HgaI GACGC 3 cut(s) 98, 268, 424
HhaI GCGC 2 cut(s) 161, 243
Hin1I GRCGYC 5 cut(s) 81, 90, 159, 279, 435
Hin1II CATG 3 cut(s) 286, 358, 403
Hin6I GCGC 2 cut(s) 159, 241
HinP1I GCGC 2 cut(s) 159, 241
HincII GTYRAC 3 cut(s) 30, 39, 439
HindII GTYRAC 3 cut(s) 30, 39, 439
HinfI GANTC 2 cut(s) 144, 200
HpaII CCGG 6 cut(s) 12, 141, 156, 178, 374, 447
Hpy166II GTNNAC 3 cut(s) 30, 39, 439
Hpy188I TCNGA 4 cut(s) 61, 211, 258, 407
Hpy8I GTNNAC 3 cut(s) 30, 39, 439
HpyAV CCTTC 1 cut(s) 142
HpyCH4IV ACGT 4 cut(s) 26, 35, 81, 291
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 330
HpyF3I CTNAG 1 cut(s) 329
HpySE526I ACGT 4 cut(s) 26, 35, 81, 291
Hsp92I GRCGYC 5 cut(s) 81, 90, 159, 279, 435
Hsp92II CATG 3 cut(s) 286, 358, 403
HspAI GCGC 2 cut(s) 159, 241
KasI GGCGCC 1 cut(s) 158
Ksp22I TGATCA 2 cut(s) 402, 472
Kzo9I GATC 5 cut(s) 108, 121, 402, 459, 472
LmnI GCTCC 1 cut(s) 158
MaeI CTAG 1 cut(s) 484
MaeII ACGT 4 cut(s) 26, 35, 81, 291
MalI GATC 5 cut(s) 110, 123, 404, 461, 474
MboI GATC 5 cut(s) 108, 121, 402, 459, 472
MboII GAAGA 3 cut(s) 36, 350, 469
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 249
Mly113I GGCGCC 1 cut(s) 159
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 3 cut(s) 84, 228, 234
MnlI CCTC 8 cut(s) 52, 61, 67, 74, 95, 182, 274, 280
MseI TTAA 1 cut(s) 344
MspI CCGG 6 cut(s) 12, 141, 156, 178, 374, 447
MspR9I CCNGG 3 cut(s) 179, 448, 464
Mva1269I GAATGC 1 cut(s) 74
MvaI CCWGG 1 cut(s) 464
MvnI CGCG 3 cut(s) 99, 243, 312
MwoI GCNNNNNNNGC 2 cut(s) 111, 330
NarI GGCGCC 1 cut(s) 159
NciI CCSGG 2 cut(s) 179, 448
NdeII GATC 5 cut(s) 108, 121, 402, 459, 472
NlaIII CATG 3 cut(s) 286, 358, 403
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 245
PcsI WCGNNNNNNNCGW 6 cut(s) 32, 84, 96, 219, 225, 234
PctI GAATGC 1 cut(s) 74
PfeI GAWTC 1 cut(s) 144
PkrI GCNGC 5 cut(s) 196, 278, 397, 416, 419
PleI GAGTC 1 cut(s) 194
PluTI GGCGCC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 307
PsrI GAACNNNNNNTAC 2 cut(s) 218, 250
PsuI RGATCY 1 cut(s) 459
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SalI GTCGAC 2 cut(s) 28, 437
SaqAI TTAA 1 cut(s) 344
SatI GCNGC 5 cut(s) 195, 277, 396, 415, 418
Sau3AI GATC 5 cut(s) 108, 121, 402, 459, 472
Sau96I GGNCC 1 cut(s) 307
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 3 cut(s) 179, 448, 464
SduI GDGCHC 1 cut(s) 249
SfoI GGCGCC 1 cut(s) 160
SgrDI CGTCGACG 1 cut(s) 28
SinI GGWCC 1 cut(s) 307
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
SspDI GGCGCC 1 cut(s) 158
SspMI CTAG 1 cut(s) 484
StyD4I CCNGG 3 cut(s) 177, 446, 462
TaiI ACGT 4 cut(s) 29, 38, 84, 294
TaqI TCGA 6 cut(s) 29, 78, 87, 222, 237, 438
TauI GCSGC 5 cut(s) 197, 279, 398, 417, 420
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TscAI CASTG 1 cut(s) 277
TspDTI ATGAA 2 cut(s) 371, 438
TspRI CASTG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XmiI GTMKAC 2 cut(s) 29, 438
XspI CTAG 1 cut(s) 484
ZraI GACGTC 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.