Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0015336

Physical Location & Seq

Genomic Coordinates Reverse (-)

12007509 .. 12008000

Gene Structure 492 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1152300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 492 bp

ATGTGTCCCTCCGGCCGAACTCTCAGCGTGGAGACCGCGACGACTACGCTCACCTCTGACTTCCGTCCAGCATTTGACGTTCTGGACGCGGACCACGACGGCAAGATCAGCAAAGACGACCTCCGAGCCTTCTACGCCGGCTTCTCCAGCTCCGCCGCGGACGAAAACCTCATCAGATCGATGATTTCTGCCGCCGATTCCGACAACGACGGGTTCGTCCAGTACGAGGAGTTCGAGCGCGTCTTGGCTTGCGGTGACGGGGAGAAAAGCTCGGGAGTGATGGATGACGTGTTCAAGGTGATGGACAAGGACGGCGACGGGAAGCTCAGCCACGAGGACCTGAAGAACTACATGAGCAGCGCCGGCTTCGTCGCCACCGATGAAGATATCAAGGCCATGATTAAACTGGGCGGCGGCGATGAGAACCAAGGCGTGACGTATGCTGGCTTGCTTAAGATCCTGGCCGTTGATCAAGTCGGCGAAGCGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0009628 GO:0009651 GO:0042538 GO:0043167 GO:0043169 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko04014 ko04015 ko04016 ko04020 ko04022 ko04024 ko04070 ko04114 ko04218 ko04261 ko04270 ko04371 ko04626 ko04713 ko04720 ko04722 ko04728 ko04740 ko04744 ko04745 ko04750 ko04910 ko04912 ko04915 ko04916 ko04921 ko04922 ko04924 ko04925 ko04970 ko04971 ko05010 ko05031 ko05034 ko05133 ko05152 ko05167 ko05200 ko05214 ko05418 map04014 map04015 map04016 map04020 map04022 map04024 map04070 map04114 map04218 map04261 map04270 map04371 map04626 map04713 map04720 map04722 map04728 map04740 map04744 map04745 map04750 map04910 map04912 map04915 map04916 map04921 map04922 map04924 map04925 map04970 map04971 map05010 map05031 map05034 map05133 map05152 map05167 map05200 map05214 map05418

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

164

Amino Acids

17.43

Weight (kDa)

4.24

Isoelectric Point (pI)

32.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	20 - 81	2.7e-13	EF-hand domain pair
EF-hand_5	PF13202	24 - 44	2.3e-06	EF hand
EF-hand_1	PF00036	94 - 120	7e-07	EF hand domain
EF-hand_5	PF13202	94 - 116	3.4e-06	EF hand
EF-hand_6	PF13405	95 - 122	1.9e-06	EF-hand domain
EF-hand_7	PF13499	96 - 148	1.3e-06	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49480
fragaria_vesca	FvH4_4g19970
malus_domestica	MD04G1036600.v1.1 MD13G1151300.v1.1 MD16G1152300.v1.1
prunus_persica	Prupe.1G115600_v2.0.a1
pyrus_communis	pycom16g12960
rosa_chinensis	RchiOBHm_Chr4g0425671
rosa_laevigata	RLG00000007348
rosa_multiflora	Rmu_co8455781.1_g000002
rosa_rugosa	Rorug04G0204700
rosa_samantha	Rh4AG262000 Rh4BG268100 Rh4CG279200 Rh4DG263800
rosa_wichuraiana	Rw4G022640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	215
AccII	CGCG	4 cut(s)	38, 89, 158, 240
AciI	CCGC	9 cut(s)	36, 89, 153, 156, 158, 192, 252, 411, 414
AclWI	GGATC	1 cut(s)	451
AcoI	YGGCCR	2 cut(s)	13, 462
AcuI	CTGAAG	1 cut(s)	362
AfaI	GTAC	1 cut(s)	224
AfiI	CCNNNNNNNGG	1 cut(s)	226
AflII	CTTAAG	1 cut(s)	452
AflIII	ACRYGT	1 cut(s)	288
AgsI	TTSAA	1 cut(s)	295
AjiI	CACGTC	1 cut(s)	289
AjnI	CCWGG	1 cut(s)	459
AloI	GAACNNNNNNTCC	2 cut(s)	275, 307
AluBI	AGCT	3 cut(s)	150, 270, 325
AluI	AGCT	3 cut(s)	150, 270, 325
Alw26I	GTCTC	1 cut(s)	26
AlwI	GGATC	1 cut(s)	451
Ama87I	CYCGRG	1 cut(s)	271
AoxI	GGCC	3 cut(s)	13, 393, 462
ApeKI	GCWGC	1 cut(s)	357
AspLEI	GCGC	3 cut(s)	240, 362, 487
AspS9I	GGNCC	2 cut(s)	91, 337
AsuHPI	GGTGA	3 cut(s)	43, 266, 310
AvaI	CYCGRG	1 cut(s)	271
AvaII	GGWCC	2 cut(s)	91, 337
BauI	CACGAG	1 cut(s)	332
BbvI	GCAGC	1 cut(s)	369
BccI	CCATC	2 cut(s)	274, 295
BceAI	ACGGC	3 cut(s)	115, 328, 449
BciT130I	CCWGG	1 cut(s)	461
BclI	TGATCA	1 cut(s)	469
BcoDI	GTCTC	1 cut(s)	26
BfoI	RGCGCY	2 cut(s)	363, 488
BfrI	CTTAAG	1 cut(s)	452
BisI	GCNGC	5 cut(s)	156, 192, 358, 412, 415
BlpI	GCTNAGC	1 cut(s)	326
BlsI	GCNGC	5 cut(s)	157, 193, 359, 413, 416
Bme1390I	CCNGG	1 cut(s)	461
Bme18I	GGWCC	2 cut(s)	91, 337
BmeT110I	CYCGRG	1 cut(s)	271
BmgBI	CACGTC	1 cut(s)	289
BmgT120I	GGNCC	2 cut(s)	91, 337
BmrFI	CCNGG	1 cut(s)	461
BmrI	ACTGGG	1 cut(s)	416
BmuI	ACTGGG	1 cut(s)	416
BoxI	GACNNNNGTC	1 cut(s)	63
BplI	GAGNNNNNCTC	2 cut(s)	254, 286
BpmI	CTGGAG	1 cut(s)	130
Bpu1102I	GCTNAGC	1 cut(s)	326
Bsa29I	ATCGAT	1 cut(s)	179
BsaI	GGTCTC	1 cut(s)	26
BsaJI	CCNNGG	2 cut(s)	156, 427
Bsc4I	CCNNNNNNNGG	1 cut(s)	226
Bse118I	RCCGGY	2 cut(s)	137, 362
Bse1I	ACTGG	2 cut(s)	220, 411
BseBI	CCWGG	1 cut(s)	461
BseCI	ATCGAT	1 cut(s)	179
BseDI	CCNNGG	2 cut(s)	156, 427
BseGI	GGATG	1 cut(s)	289
BseLI	CCNNNNNNNGG	1 cut(s)	226
BseMII	CTCAG	2 cut(s)	37, 340
BseNI	ACTGG	2 cut(s)	220, 411
BseRI	GAGGAG	1 cut(s)	242
BseX3I	CGGCCG	1 cut(s)	13
BseXI	GCAGC	1 cut(s)	369
Bsh1236I	CGCG	4 cut(s)	38, 89, 158, 240
Bsh1285I	CGRYCG	1 cut(s)	16
BshFI	GGCC	3 cut(s)	15, 395, 464
BshVI	ATCGAT	1 cut(s)	179
BsiEI	CGRYCG	1 cut(s)	16
BsiHKCI	CYCGRG	1 cut(s)	271
BsiSI	CCGG	3 cut(s)	12, 138, 363
BslI	CCNNNNNNNGG	1 cut(s)	226
BsmAI	GTCTC	1 cut(s)	26
BsnI	GGCC	3 cut(s)	15, 395, 464
Bso31I	GGTCTC	1 cut(s)	26
BsoBI	CYCGRG	1 cut(s)	271
Bsp143I	GATC	4 cut(s)	105, 176, 456, 469
Bsp1720I	GCTNAGC	1 cut(s)	326
BspACI	CCGC	9 cut(s)	36, 89, 153, 156, 158, 192, 252, 411, 414
BspANI	GGCC	3 cut(s)	15, 395, 464
BspCNI	CTCAG	2 cut(s)	36, 339
BspDI	ATCGAT	1 cut(s)	179
BspFNI	CGCG	4 cut(s)	38, 89, 158, 240
BspPI	GGATC	1 cut(s)	451
BspTI	CTTAAG	1 cut(s)	452
BspTNI	GGTCTC	1 cut(s)	26
BsrFI	RCCGGY	2 cut(s)	137, 362
BsrI	ACTGG	2 cut(s)	220, 411
BssAI	RCCGGY	2 cut(s)	137, 362
BssECI	CCNNGG	2 cut(s)	156, 427
BssMI	GATC	4 cut(s)	105, 176, 456, 469
BssSI	CACGAG	1 cut(s)	332
BssT1I	CCWWGG	1 cut(s)	427
Bst2BI	CACGAG	1 cut(s)	332
Bst2UI	CCWGG	1 cut(s)	461
BstAFI	CTTAAG	1 cut(s)	452
BstC8I	GCNNGC	5 cut(s)	139, 250, 364, 445, 449
BstDEI	CTNAG	2 cut(s)	23, 326
BstDSI	CCRYGG	1 cut(s)	156
BstF5I	GGATG	1 cut(s)	289
BstFNI	CGCG	4 cut(s)	38, 89, 158, 240
BstH2I	RGCGCY	2 cut(s)	363, 488
BstHHI	GCGC	3 cut(s)	240, 362, 487
BstKTI	GATC	4 cut(s)	108, 179, 459, 472
BstMAI	GTCTC	1 cut(s)	26
BstMBI	GATC	4 cut(s)	105, 176, 456, 469
BstMCI	CGRYCG	1 cut(s)	16
BstMWI	GCNNNNNNNGC	4 cut(s)	108, 134, 147, 363
BstNI	CCWGG	1 cut(s)	461
BstPAI	GACNNNNGTC	1 cut(s)	63
BstSCI	CCNGG	1 cut(s)	459
BstUI	CGCG	4 cut(s)	38, 89, 158, 240
BstV1I	GCAGC	1 cut(s)	369
BstX2I	RGATCY	1 cut(s)	456
BstYI	RGATCY	1 cut(s)	456
BstZI	CGGCCG	1 cut(s)	13
Bsu15I	ATCGAT	1 cut(s)	179
BsuRI	GGCC	3 cut(s)	15, 395, 464
BsuTUI	ATCGAT	1 cut(s)	179
BtgI	CCRYGG	1 cut(s)	156
BtgZI	GCGATG	1 cut(s)	432
BtrI	CACGTC	1 cut(s)	289
BtsCI	GGATG	1 cut(s)	289
Cac8I	GCNNGC	5 cut(s)	139, 250, 364, 445, 449
CfoI	GCGC	3 cut(s)	240, 362, 487
Cfr10I	RCCGGY	2 cut(s)	137, 362
Cfr13I	GGNCC	2 cut(s)	91, 337
Cfr42I	CCGCGG	1 cut(s)	159
ClaI	ATCGAT	1 cut(s)	179
CseI	GACGC	2 cut(s)	95, 229
Csp6I	GTAC	1 cut(s)	223
CviAII	CATG	2 cut(s)	352, 397
CviQI	GTAC	1 cut(s)	223
DdeI	CTNAG	2 cut(s)	23, 326
DpnI	GATC	4 cut(s)	107, 178, 458, 471
DpnII	GATC	4 cut(s)	105, 176, 456, 469
DrdI	GACNNNNNNGTC	1 cut(s)	215
DseDI	GACNNNNNNGTC	1 cut(s)	215
EaeI	YGGCCR	2 cut(s)	13, 462
EagI	CGGCCG	1 cut(s)	13
EciI	GGCGGA	1 cut(s)	142
EclXI	CGGCCG	1 cut(s)	13
Eco130I	CCWWGG	1 cut(s)	427
Eco31I	GGTCTC	1 cut(s)	26
Eco32I	GATATC	1 cut(s)	388
Eco47I	GGWCC	2 cut(s)	91, 337
Eco52I	CGGCCG	1 cut(s)	13
Eco57I	CTGAAG	1 cut(s)	362
Eco88I	CYCGRG	1 cut(s)	271
EcoO109I	RGGNCCY	1 cut(s)	337
EcoRII	CCWGG	1 cut(s)	459
EcoRV	GATATC	1 cut(s)	388
EcoT14I	CCWWGG	1 cut(s)	427
ErhI	CCWWGG	1 cut(s)	427
FaeI	CATG	2 cut(s)	355, 400
FaiI	YATR	3 cut(s)	353, 398, 441
FatI	CATG	2 cut(s)	351, 396
FbaI	TGATCA	1 cut(s)	469
Fnu4HI	GCNGC	5 cut(s)	156, 192, 358, 412, 415
FokI	GGATG	1 cut(s)	296
Fsp4HI	GCNGC	5 cut(s)	156, 192, 358, 412, 415
GlaI	GCGC	3 cut(s)	239, 361, 486
GluI	GCNGC	5 cut(s)	156, 192, 358, 412, 415
GsuI	CTGGAG	1 cut(s)	130
HaeII	RGCGCY	2 cut(s)	363, 488
HaeIII	GGCC	3 cut(s)	15, 395, 464
HapII	CCGG	3 cut(s)	12, 138, 363
HgaI	GACGC	2 cut(s)	95, 229
HhaI	GCGC	3 cut(s)	240, 362, 487
Hin1II	CATG	2 cut(s)	355, 400
Hin6I	GCGC	3 cut(s)	238, 360, 485
HinP1I	GCGC	3 cut(s)	238, 360, 485
HinfI	GANTC	1 cut(s)	197
HpaII	CCGG	3 cut(s)	12, 138, 363
HphI	GGTGA	3 cut(s)	43, 266, 310
Hpy188I	TCNGA	4 cut(s)	58, 125, 176, 202
Hpy188III	TCNNGA	2 cut(s)	83, 273
Hpy99I	CGWCG	5 cut(s)	43, 101, 212, 320, 374
HpyAV	CCTTC	1 cut(s)	139
HpyCH4IV	ACGT	3 cut(s)	78, 288, 437
HpyF10VI	GCNNNNNNNGC	4 cut(s)	108, 134, 147, 363
HpyF3I	CTNAG	2 cut(s)	23, 326
HpySE526I	ACGT	3 cut(s)	78, 288, 437
Hsp92II	CATG	2 cut(s)	355, 400
HspAI	GCGC	3 cut(s)	238, 360, 485
KroI	GCCGGC	2 cut(s)	137, 362
KroNI	GCCGGC	2 cut(s)	139, 364
Ksp22I	TGATCA	1 cut(s)	469
KspI	CCGCGG	1 cut(s)	159
Kzo9I	GATC	4 cut(s)	105, 176, 456, 469
LmnI	GCTCC	1 cut(s)	155
Lsp1109I	GCAGC	1 cut(s)	369
MaeII	ACGT	3 cut(s)	78, 288, 437
MaeIII	GTNAC	2 cut(s)	254, 433
MalI	GATC	4 cut(s)	107, 178, 458, 471
MboI	GATC	4 cut(s)	105, 176, 456, 469
MboII	GAAGA	2 cut(s)	355, 395
MflI	RGATCY	1 cut(s)	456
MmeI	TCCRAC	1 cut(s)	225
MnlI	CCTC	6 cut(s)	19, 64, 131, 179, 220, 328
MroNI	GCCGGC	2 cut(s)	137, 362
MseI	TTAA	2 cut(s)	402, 453
MspA1I	CMGCKG	1 cut(s)	158
MspCI	CTTAAG	1 cut(s)	452
MspI	CCGG	3 cut(s)	12, 138, 363
MspR9I	CCNGG	1 cut(s)	461
MvaI	CCWGG	1 cut(s)	461
MvnI	CGCG	4 cut(s)	38, 89, 158, 240
MwoI	GCNNNNNNNGC	4 cut(s)	108, 134, 147, 363
NaeI	GCCGGC	2 cut(s)	139, 364
NdeII	GATC	4 cut(s)	105, 176, 456, 469
NgoMIV	GCCGGC	2 cut(s)	137, 362
NlaIII	CATG	2 cut(s)	355, 400
NmuCI	GTSAC	2 cut(s)	254, 433
PcsI	WCGNNNNNNNCGW	5 cut(s)	93, 213, 222, 231, 375
PdiI	GCCGGC	2 cut(s)	139, 364
PfeI	GAWTC	1 cut(s)	197
PkrI	GCNGC	5 cut(s)	157, 193, 359, 413, 416
PpuMI	RGGWCCY	1 cut(s)	337
PshAI	GACNNNNGTC	1 cut(s)	63
Psp5II	RGGWCCY	1 cut(s)	337
Psp6I	CCWGG	1 cut(s)	459
PspGI	CCWGG	1 cut(s)	459
PspPI	GGNCC	2 cut(s)	91, 337
PspPPI	RGGWCCY	1 cut(s)	337
PsrI	GAACNNNNNNTAC	2 cut(s)	215, 247
PsuI	RGATCY	1 cut(s)	456
RsaI	GTAC	1 cut(s)	224
RsaNI	GTAC	1 cut(s)	223
SacII	CCGCGG	1 cut(s)	159
SaqAI	TTAA	2 cut(s)	402, 453
SatI	GCNGC	5 cut(s)	156, 192, 358, 412, 415
Sau3AI	GATC	4 cut(s)	105, 176, 456, 469
Sau96I	GGNCC	2 cut(s)	91, 337
ScrFI	CCNGG	1 cut(s)	461
Sfr303I	CCGCGG	1 cut(s)	159
SgrBI	CCGCGG	1 cut(s)	159
SinI	GGWCC	2 cut(s)	91, 337
SmlI	CTYRAG	1 cut(s)	452
SmoI	CTYRAG	1 cut(s)	452
SsiI	CCGC	9 cut(s)	36, 89, 153, 156, 158, 192, 252, 411, 414
StyD4I	CCNGG	1 cut(s)	459
StyI	CCWWGG	1 cut(s)	427
TaiI	ACGT	3 cut(s)	81, 291, 440
TaqI	TCGA	2 cut(s)	179, 234
TauI	GCSGC	4 cut(s)	158, 194, 414, 417
TfiI	GAWTC	1 cut(s)	197
Tru1I	TTAA	2 cut(s)	402, 453
Tru9I	TTAA	2 cut(s)	402, 453
TseFI	GTSAC	2 cut(s)	254, 433
TseI	GCWGC	1 cut(s)	357
Tsp45I	GTSAC	2 cut(s)	254, 433
TspDTI	ATGAA	1 cut(s)	396
TspGWI	ACGGA	1 cut(s)	53
Vha464I	CTTAAG	1 cut(s)	452
VpaK11BI	GGWCC	2 cut(s)	91, 337
XcmI	CCANNNNNNNNNTGG	1 cut(s)	403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.