Rorug04G0204700

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015336
Physical Location & Seq
Reverse (-)
35702036 .. 35702397
362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0204700.1

Sequence Viewer

Length: 333 bp
ATGAAGAGGAACCCGCGCGTCTCGAGCTCCAGGCGCAAGAGCCGCAAGGCCCACTTCACGGCGCCGTCGAGCGTCCGCCGCGTCCTGATGAGCGCGCCGCTCTCCTCCGACCTCCGGTCGAAGTACAACGTCCGGTCCATGCCGGTGCGCAAGGACGACAAGGTCCAGGTGGTGCGTGGGACCTATAAGGTCCGCGAGGGCAAGGTGGTCCAGGTCTACCGCGGCAAGTGGGTCATTGAAAATACAAGGACAGAATATGATTTTCTGGAAAACGAGGATTGCAGTGCACTGATCAATCCTTACTGGGCAGCAGAAACCAAAATAACAAACTAA

Protein Analysis

110

Amino Acids

12.82

Weight (kDa)

10.4

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L26 PF16906 8 - 67 8.3e-21 Ribosomal proteins L26 eukaryotic, L24P archaeal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49480
fragaria_vesca FvH4_4g19970
malus_domestica MD04G1036600.v1.1 MD13G1151300.v1.1 MD16G1152300.v1.1
prunus_persica Prupe.1G115600_v2.0.a1
pyrus_communis pycom16g12960
rosa_chinensis RchiOBHm_Chr4g0425671
rosa_laevigata RLG00000007348
rosa_multiflora Rmu_co8455781.1_g000002
rosa_rugosa Rorug04G0204700
rosa_samantha Rh4AG262000 Rh4BG268100 Rh4CG279200 Rh4DG263800
rosa_wichuraiana Rw4G022640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
Acc16I TGCGCA 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 61
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 216
AccII CGCG 6 cut(s) 16, 18, 81, 95, 195, 222
AciI CCGC 8 cut(s) 14, 43, 76, 79, 98, 193, 220, 222
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 58, 114
AgsI TTSAA 1 cut(s) 239
AhdI GACNNNNNGTC 1 cut(s) 115
AjnI CCWGG 3 cut(s) 29, 165, 210
AluBI AGCT 1 cut(s) 27
AluI AGCT 1 cut(s) 27
Alw21I GWGCWC 2 cut(s) 29, 289
Alw26I GTCTC 1 cut(s) 25
Alw44I GTGCAC 1 cut(s) 285
Ama87I CYCGRG 1 cut(s) 22
AoxI GGCC 1 cut(s) 48
ApaLI GTGCAC 1 cut(s) 285
ApeKI GCWGC 1 cut(s) 308
AspLEI GCGC 6 cut(s) 18, 36, 64, 95, 97, 150
AspS9I GGNCC 6 cut(s) 49, 135, 163, 180, 190, 208
AvaI CYCGRG 1 cut(s) 22
AvaII GGWCC 5 cut(s) 135, 163, 180, 190, 208
BaeGI GKGCMC 1 cut(s) 289
BanI GGYRCC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 29
Bbv12I GWGCWC 2 cut(s) 29, 289
BbvI GCAGC 1 cut(s) 320
BceAI ACGGC 2 cut(s) 49, 75
BciT130I CCWGG 3 cut(s) 31, 167, 212
BclI TGATCA 1 cut(s) 291
BcoDI GTCTC 1 cut(s) 25
BfoI RGCGCY 1 cut(s) 65
BisI GCNGC 5 cut(s) 43, 79, 98, 223, 309
BlsI GCNGC 5 cut(s) 44, 80, 99, 224, 310
Bme1390I CCNGG 3 cut(s) 31, 167, 212
Bme18I GGWCC 5 cut(s) 135, 163, 180, 190, 208
BmeRI GACNNNNNGTC 1 cut(s) 115
BmeT110I CYCGRG 1 cut(s) 22
BmgT120I GGNCC 6 cut(s) 49, 135, 163, 180, 190, 208
BmiI GGNNCC 3 cut(s) 11, 63, 181
BmrFI CCNGG 3 cut(s) 31, 167, 212
BmrI ACTGGG 1 cut(s) 313
BmuI ACTGGG 1 cut(s) 313
BpmI CTGGAG 1 cut(s) 13
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 220
BsaWI WCCGGW 2 cut(s) 114, 132
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 114
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 308
BseBI CCWGG 3 cut(s) 31, 167, 212
BseDI CCNNGG 1 cut(s) 220
BseLI CCNNNNNNNGG 2 cut(s) 58, 114
BseNI ACTGG 1 cut(s) 308
BsePI GCGCGC 1 cut(s) 93
BseRI GAGGAG 1 cut(s) 94
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 1 cut(s) 320
Bsh1236I CGCG 6 cut(s) 16, 18, 81, 95, 195, 222
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 61
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 2 cut(s) 29, 289
BsiHKCI CYCGRG 1 cut(s) 22
BsiSI CCGG 3 cut(s) 115, 133, 143
BslFI GGGAC 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 58, 114
BsmAI GTCTC 1 cut(s) 25
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 22
Bsp1286I GDGCHC 2 cut(s) 29, 289
Bsp143I GATC 1 cut(s) 291
BspACI CCGC 8 cut(s) 14, 43, 76, 79, 98, 193, 220, 222
BspANI GGCC 1 cut(s) 50
BspFNI CGCG 6 cut(s) 16, 18, 81, 95, 195, 222
BspLI GGNNCC 3 cut(s) 11, 63, 181
BspT107I GGYRCC 1 cut(s) 61
BsrBI CCGCTC 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 1 cut(s) 308
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 220
BssHII GCGCGC 1 cut(s) 93
BssMI GATC 1 cut(s) 291
BssNI GRCGYC 1 cut(s) 62
Bst2UI CCWGG 3 cut(s) 31, 167, 212
BstACI GRCGYC 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 95
BstDSI CCRYGG 1 cut(s) 220
BstFNI CGCG 6 cut(s) 16, 18, 81, 95, 195, 222
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 6 cut(s) 18, 36, 64, 95, 97, 150
BstKTI GATC 1 cut(s) 294
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 1 cut(s) 291
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 4 cut(s) 24, 33, 42, 78
BstNI CCWGG 3 cut(s) 31, 167, 212
BstSCI CCNGG 3 cut(s) 29, 165, 210
BstSLI GKGCMC 1 cut(s) 289
BstUI CGCG 6 cut(s) 16, 18, 81, 95, 195, 222
BstV1I GCAGC 1 cut(s) 320
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 220
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 2 cut(s) 287, 289
Cac8I GCNNGC 1 cut(s) 95
CfoI GCGC 6 cut(s) 18, 36, 64, 95, 97, 150
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 6 cut(s) 49, 135, 163, 180, 190, 208
Cfr42I CCGCGG 1 cut(s) 223
CseI GACGC 3 cut(s) 7, 61, 70
Csp6I GTAC 1 cut(s) 124
CviAII CATG 1 cut(s) 139
CviJI RGCY 3 cut(s) 27, 42, 50
CviKI_1 RGCY 3 cut(s) 27, 42, 50
CviQI GTAC 1 cut(s) 124
DinI GGCGCC 1 cut(s) 63
DpnI GATC 1 cut(s) 293
DpnII GATC 1 cut(s) 291
DrdI GACNNNNNNGTC 1 cut(s) 161
DriI GACNNNNNGTC 1 cut(s) 115
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1105I GACNNNNNGTC 1 cut(s) 115
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 29
Eco47I GGWCC 5 cut(s) 135, 163, 180, 190, 208
Eco53kI GAGCTC 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 27
EcoO109I RGGNCCY 1 cut(s) 180
EcoRII CCWGG 3 cut(s) 29, 165, 210
EcoT38I GRGCYC 1 cut(s) 29
EgeI GGCGCC 1 cut(s) 63
EheI GGCGCC 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 1 cut(s) 142
FaiI YATR 3 cut(s) 140, 186, 258
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 1 cut(s) 193
FatI CATG 1 cut(s) 138
FauI CCCGC 1 cut(s) 21
FbaI TGATCA 1 cut(s) 291
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 5 cut(s) 43, 79, 98, 223, 309
FriOI GRGCYC 1 cut(s) 29
Fsp4HI GCNGC 5 cut(s) 43, 79, 98, 223, 309
FspI TGCGCA 1 cut(s) 149
GlaI GCGC 6 cut(s) 17, 35, 63, 94, 96, 149
GluI GCNGC 5 cut(s) 43, 79, 98, 223, 309
GsuI CTGGAG 1 cut(s) 13
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 1 cut(s) 50
HapII CCGG 3 cut(s) 115, 133, 143
HgaI GACGC 3 cut(s) 7, 61, 70
HhaI GCGC 6 cut(s) 18, 36, 64, 95, 97, 150
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 1 cut(s) 142
Hin6I GCGC 6 cut(s) 16, 34, 62, 93, 95, 148
HinP1I GCGC 6 cut(s) 16, 34, 62, 93, 95, 148
HpaII CCGG 3 cut(s) 115, 133, 143
Hpy166II GTNNAC 2 cut(s) 217, 287
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 3 cut(s) 22, 85, 266
Hpy8I GTNNAC 2 cut(s) 217, 287
Hpy99I CGWCG 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 2 cut(s) 282, 287
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 33, 42, 78
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 1 cut(s) 142
HspAI GCGC 6 cut(s) 16, 34, 62, 93, 95, 148
KasI GGCGCC 1 cut(s) 61
Ksp22I TGATCA 1 cut(s) 291
KspI CCGCGG 1 cut(s) 223
Kzo9I GATC 1 cut(s) 291
LmnI GCTCC 1 cut(s) 32
Lsp1109I GCAGC 1 cut(s) 320
MaeII ACGT 1 cut(s) 129
MalI GATC 1 cut(s) 293
MbiI CCGCTC 1 cut(s) 100
MboI GATC 1 cut(s) 291
MboII GAAGA 1 cut(s) 16
MhlI GDGCHC 2 cut(s) 29, 289
Mly113I GGCGCC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 4 cut(s) 115, 122, 190, 268
MslI CAYNNNNRTG 1 cut(s) 143
MspA1I CMGCKG 1 cut(s) 222
MspI CCGG 3 cut(s) 115, 133, 143
MspR9I CCNGG 3 cut(s) 31, 167, 212
MvaI CCWGG 3 cut(s) 31, 167, 212
MvnI CGCG 6 cut(s) 16, 18, 81, 95, 195, 222
MwoI GCNNNNNNNGC 4 cut(s) 24, 33, 42, 78
NarI GGCGCC 1 cut(s) 62
NdeII GATC 1 cut(s) 291
NlaIII CATG 1 cut(s) 142
NlaIV GGNNCC 3 cut(s) 11, 63, 181
NsbI TGCGCA 1 cut(s) 149
PaeR7I CTCGAG 1 cut(s) 22
PauI GCGCGC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 65
PflFI GACNNNGTC 1 cut(s) 161
PkrI GCNGC 5 cut(s) 44, 80, 99, 224, 310
PluTI GGCGCC 1 cut(s) 65
PpuMI RGGWCCY 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 29
Psp5II RGGWCCY 1 cut(s) 180
Psp6I CCWGG 3 cut(s) 29, 165, 210
PspGI CCWGG 3 cut(s) 29, 165, 210
PspN4I GGNNCC 3 cut(s) 11, 63, 181
PspPI GGNCC 6 cut(s) 49, 135, 163, 180, 190, 208
PspPPI RGGWCCY 1 cut(s) 180
PsyI GACNNNGTC 1 cut(s) 161
PteI GCGCGC 1 cut(s) 93
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 143
SacI GAGCTC 1 cut(s) 29
SacII CCGCGG 1 cut(s) 223
SatI GCNGC 5 cut(s) 43, 79, 98, 223, 309
Sau3AI GATC 1 cut(s) 291
Sau96I GGNCC 6 cut(s) 49, 135, 163, 180, 190, 208
ScrFI CCNGG 3 cut(s) 31, 167, 212
SduI GDGCHC 2 cut(s) 29, 289
SetI ASST 9 cut(s) 29, 114, 132, 165, 171, 185, 192, 207, 216
SfoI GGCGCC 1 cut(s) 63
Sfr274I CTCGAG 1 cut(s) 22
Sfr303I CCGCGG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 223
SinI GGWCC 5 cut(s) 135, 163, 180, 190, 208
SlaI CTCGAG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 22
SmoI CTYRAG 1 cut(s) 22
SsiI CCGC 8 cut(s) 14, 43, 76, 79, 98, 193, 220, 222
SspDI GGCGCC 1 cut(s) 61
SstI GAGCTC 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 29, 165, 210
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 23, 68, 119
TatI WGTACW 1 cut(s) 123
TauI GCSGC 4 cut(s) 45, 81, 100, 225
TscAI CASTG 2 cut(s) 289, 294
TseI GCWGC 1 cut(s) 308
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 289, 294
Tth111I GACNNNGTC 1 cut(s) 161
VneI GTGCAC 1 cut(s) 285
VpaK11BI GGWCC 5 cut(s) 135, 163, 180, 190, 208
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XhoI CTCGAG 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.