MD13G1151300.v1.1

EF-hand domain pair

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015336
Physical Location & Seq
Reverse (-)
11891185 .. 11891688
504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1151300.v1.1.491

Sequence Viewer

Length: 504 bp
ATGTGTCCCTCCGGCCGAACGCTCAGCTCGCAGACGGAGACGACGGCGCTCACCTCCGACTTCCTTCCAGCATTCAACGTCCTGGACGCCGACCGCGACGGAAAGATCAGCAAAGACGACCTCCGAGCCTTCTACGCCGGCTTCTCCAGCTCCGGCGTTGACGAAAACCTCATCGGTTCAATGATGTCGGCGGCCGACTCCGACAACGACGGCTTCGTCCAGTACGACGAGTTCGAGCGCGTTTTGGCGGGCGGCCGCTGGCCGAGAAGCTCGGGAGTGATGGAGGACGTGTTCAAGGTGATGGACAAGGACGGGGACGGCAAGCTGAGCCACGAGGATCTGAAGAGCTACATGAGCAGCGCAGGCTTTGCCGCCAGCGACGAAGAAATCAAGGCCATGATAAAACTGGGCGGTGGCGATGAGAACCAAGGGGTGACTTACGCTGGCTTGCTTAAGATCCTGGCCGTTGATCAAGTCGGCCAAGTCCAAGTTGCCAGGTCTTAA

Protein Analysis

168

Amino Acids

17.89

Weight (kDa)

4.38

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 22 - 81 3.5e-11 EF-hand domain pair
EF-hand_5 PF13202 24 - 44 2.8e-06 EF hand
EF-hand_7 PF13499 94 - 150 1.2e-07 EF-hand domain pair
EF-hand_5 PF13202 94 - 116 4.9e-06 EF hand
EF-hand_6 PF13405 94 - 122 2e-06 EF-hand domain
EF-hand_1 PF00036 95 - 120 1e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015336)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49480
fragaria_vesca FvH4_4g19970
malus_domestica MD04G1036600.v1.1 MD13G1151300.v1.1 MD16G1152300.v1.1
prunus_persica Prupe.1G115600_v2.0.a1
pyrus_communis pycom16g12960
rosa_chinensis RchiOBHm_Chr4g0425671
rosa_laevigata RLG00000007348
rosa_multiflora Rmu_co8455781.1_g000002
rosa_rugosa Rorug04G0204700
rosa_samantha Rh4AG262000 Rh4BG268100 Rh4CG279200 Rh4DG263800
rosa_wichuraiana Rw4G022640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccII CGCG 2 cut(s) 96, 240
AciI CCGC 7 cut(s) 94, 191, 248, 252, 256, 372, 411
AclWI GGATC 2 cut(s) 345, 451
AcoI YGGCCR 6 cut(s) 13, 192, 253, 260, 462, 478
AcuI CTGAAG 1 cut(s) 362
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 1 cut(s) 224
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 3 cut(s) 76, 180, 295
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 3 cut(s) 81, 459, 494
AloI GAACNNNNNNTCC 2 cut(s) 275, 307
AluBI AGCT 5 cut(s) 27, 150, 270, 325, 348
AluI AGCT 5 cut(s) 27, 150, 270, 325, 348
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 345, 451
Ama87I CYCGRG 1 cut(s) 271
AoxI GGCC 7 cut(s) 13, 192, 253, 260, 393, 462, 478
ApeKI GCWGC 1 cut(s) 357
AspLEI GCGC 3 cut(s) 49, 240, 362
AsuHPI GGTGA 3 cut(s) 43, 310, 445
AvaI CYCGRG 1 cut(s) 271
BauI CACGAG 1 cut(s) 332
BbvI GCAGC 1 cut(s) 369
BccI CCATC 2 cut(s) 274, 295
BceAI ACGGC 4 cut(s) 60, 226, 334, 449
BciT130I CCWGG 3 cut(s) 83, 461, 496
BclI TGATCA 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 50
BfrI CTTAAG 1 cut(s) 452
BisI GCNGC 5 cut(s) 192, 253, 256, 358, 372
BlpI GCTNAGC 2 cut(s) 23, 326
BlsI GCNGC 5 cut(s) 193, 254, 257, 359, 373
Bme1390I CCNGG 3 cut(s) 83, 461, 496
BmeT110I CYCGRG 1 cut(s) 271
BmgBI CACGTC 1 cut(s) 289
BmrFI CCNGG 3 cut(s) 83, 461, 496
BmrI ACTGGG 1 cut(s) 416
BmuI ACTGGG 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 130
Bpu1102I GCTNAGC 2 cut(s) 23, 326
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 427
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 2 cut(s) 220, 411
BseBI CCWGG 3 cut(s) 83, 461, 496
BseDI CCNNGG 1 cut(s) 427
BseMII CTCAG 2 cut(s) 37, 317
BseNI ACTGG 2 cut(s) 220, 411
BseX3I CGGCCG 3 cut(s) 13, 192, 253
BseXI GCAGC 1 cut(s) 369
Bsh1236I CGCG 2 cut(s) 96, 240
Bsh1285I CGRYCG 4 cut(s) 16, 94, 195, 256
BshFI GGCC 7 cut(s) 15, 194, 255, 262, 395, 464, 480
BsiEI CGRYCG 4 cut(s) 16, 94, 195, 256
BsiHKCI CYCGRG 1 cut(s) 271
BsiSI CCGG 3 cut(s) 12, 138, 153
BslFI GGGAC 1 cut(s) 329
BsmAI GTCTC 1 cut(s) 32
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 7 cut(s) 15, 194, 255, 262, 395, 464, 480
BsoBI CYCGRG 1 cut(s) 271
Bsp143I GATC 4 cut(s) 105, 337, 456, 469
Bsp1720I GCTNAGC 2 cut(s) 23, 326
BspACI CCGC 7 cut(s) 94, 191, 248, 252, 256, 372, 411
BspANI GGCC 7 cut(s) 15, 194, 255, 262, 395, 464, 480
BspCNI CTCAG 2 cut(s) 36, 318
BspFNI CGCG 2 cut(s) 96, 240
BspPI GGATC 2 cut(s) 345, 451
BspQI GCTCTTC 1 cut(s) 338
BspTI CTTAAG 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 2 cut(s) 220, 411
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 4 cut(s) 105, 337, 456, 469
BssNI GRCGYC 1 cut(s) 87
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 427
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 3 cut(s) 83, 461, 496
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 87
BstAFI CTTAAG 1 cut(s) 452
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 9 cut(s) 29, 139, 250, 260, 323, 364, 376, 445, 449
BstDEI CTNAG 2 cut(s) 23, 326
BstFNI CGCG 2 cut(s) 96, 240
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 3 cut(s) 49, 240, 362
BstKTI GATC 4 cut(s) 108, 340, 459, 472
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 4 cut(s) 105, 337, 456, 469
BstMCI CGRYCG 4 cut(s) 16, 94, 195, 256
BstMWI GCNNNNNNNGC 7 cut(s) 28, 134, 147, 327, 354, 363, 368
BstNI CCWGG 3 cut(s) 83, 461, 496
BstSCI CCNGG 3 cut(s) 81, 459, 494
BstUI CGCG 2 cut(s) 96, 240
BstV1I GCAGC 1 cut(s) 369
BstX2I RGATCY 2 cut(s) 337, 456
BstYI RGATCY 2 cut(s) 337, 456
BstZI CGGCCG 3 cut(s) 13, 192, 253
BsuRI GGCC 7 cut(s) 15, 194, 255, 262, 395, 464, 480
BtgZI GCGATG 1 cut(s) 432
BtrI CACGTC 1 cut(s) 289
Cac8I GCNNGC 9 cut(s) 29, 139, 250, 260, 323, 364, 376, 445, 449
CciNI GCGGCCGC 1 cut(s) 253
CfoI GCGC 3 cut(s) 49, 240, 362
Cfr10I RCCGGY 1 cut(s) 137
CseI GACGC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 223
CviAII CATG 2 cut(s) 352, 397
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 2 cut(s) 23, 326
DpnI GATC 4 cut(s) 107, 339, 458, 471
DpnII GATC 4 cut(s) 105, 337, 456, 469
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 6 cut(s) 13, 192, 253, 260, 462, 478
EagI CGGCCG 3 cut(s) 13, 192, 253
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
EclXI CGGCCG 3 cut(s) 13, 192, 253
Eco130I CCWWGG 1 cut(s) 427
Eco52I CGGCCG 3 cut(s) 13, 192, 253
Eco57I CTGAAG 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 271
EcoRII CCWGG 3 cut(s) 81, 459, 494
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 2 cut(s) 355, 400
FaiI YATR 2 cut(s) 353, 398
FaqI GGGAC 1 cut(s) 329
FatI CATG 2 cut(s) 351, 396
FauI CCCGC 1 cut(s) 241
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 5 cut(s) 192, 253, 256, 358, 372
Fsp4HI GCNGC 5 cut(s) 192, 253, 256, 358, 372
GlaI GCGC 3 cut(s) 48, 239, 361
GluI GCNGC 5 cut(s) 192, 253, 256, 358, 372
GsuI CTGGAG 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 7 cut(s) 15, 194, 255, 262, 395, 464, 480
HapII CCGG 3 cut(s) 12, 138, 153
HgaI GACGC 1 cut(s) 95
HhaI GCGC 3 cut(s) 49, 240, 362
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 2 cut(s) 355, 400
Hin6I GCGC 3 cut(s) 47, 238, 360
HinP1I GCGC 3 cut(s) 47, 238, 360
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 1 cut(s) 197
HpaII CCGG 3 cut(s) 12, 138, 153
HphI GGTGA 3 cut(s) 43, 310, 445
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 4 cut(s) 58, 125, 202, 342
Hpy188III TCNNGA 1 cut(s) 273
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 5 cut(s) 46, 101, 212, 230, 383
HpyAV CCTTC 2 cut(s) 74, 139
HpyCH4IV ACGT 2 cut(s) 78, 288
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 134, 147, 327, 354, 363, 368
HpyF3I CTNAG 2 cut(s) 23, 326
HpySE526I ACGT 2 cut(s) 78, 288
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 2 cut(s) 355, 400
HspAI GCGC 3 cut(s) 47, 238, 360
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 469
Kzo9I GATC 4 cut(s) 105, 337, 456, 469
LguI GCTCTTC 1 cut(s) 338
LmnI GCTCC 1 cut(s) 155
Lsp1109I GCAGC 1 cut(s) 369
MaeII ACGT 2 cut(s) 78, 288
MaeIII GTNAC 1 cut(s) 433
MalI GATC 4 cut(s) 107, 339, 458, 471
MboI GATC 4 cut(s) 105, 337, 456, 469
MboII GAAGA 2 cut(s) 355, 395
MflI RGATCY 2 cut(s) 337, 456
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 2 cut(s) 81, 225
MnlI CCTC 6 cut(s) 19, 64, 131, 179, 277, 328
MroNI GCCGGC 1 cut(s) 137
MseI TTAA 2 cut(s) 453, 502
MspA1I CMGCKG 1 cut(s) 258
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 3 cut(s) 12, 138, 153
MspR9I CCNGG 3 cut(s) 83, 461, 496
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 3 cut(s) 83, 461, 496
MvnI CGCG 2 cut(s) 96, 240
MwoI GCNNNNNNNGC 7 cut(s) 28, 134, 147, 327, 354, 363, 368
NaeI GCCGGC 1 cut(s) 139
NdeII GATC 4 cut(s) 105, 337, 456, 469
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 2 cut(s) 355, 400
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 1 cut(s) 433
NotI GCGGCCGC 1 cut(s) 253
PciSI GCTCTTC 1 cut(s) 338
PcsI WCGNNNNNNNCGW 4 cut(s) 93, 213, 222, 231
PctI GAATGC 1 cut(s) 71
PdiI GCCGGC 1 cut(s) 139
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 5 cut(s) 193, 254, 257, 359, 373
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Psp6I CCWGG 3 cut(s) 81, 459, 494
PspGI CCWGG 3 cut(s) 81, 459, 494
PsrI GAACNNNNNNTAC 2 cut(s) 215, 247
PsuI RGATCY 2 cut(s) 337, 456
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SapI GCTCTTC 1 cut(s) 338
SaqAI TTAA 2 cut(s) 453, 502
SatI GCNGC 5 cut(s) 192, 253, 256, 358, 372
Sau3AI GATC 4 cut(s) 105, 337, 456, 469
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 3 cut(s) 83, 461, 496
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SsiI CCGC 7 cut(s) 94, 191, 248, 252, 256, 372, 411
StyD4I CCNGG 3 cut(s) 81, 459, 494
StyI CCWWGG 1 cut(s) 427
TaiI ACGT 2 cut(s) 81, 291
TaqI TCGA 1 cut(s) 234
TauI GCSGC 4 cut(s) 194, 255, 258, 374
Tru1I TTAA 2 cut(s) 453, 502
Tru9I TTAA 2 cut(s) 453, 502
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 433
TspGWI ACGGA 2 cut(s) 50, 114
Vha464I CTTAAG 1 cut(s) 452
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.