FvH4_4g20250

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015096
Physical Location & Seq
Reverse (-)
23582583 .. 23586227
3645 bp
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UTR
Exon/CDS
Intron
FvH4_4g20250.t1

Sequence Viewer

Length: 573 bp
ATGGCCACGTCATCATCTTCAACCGAGGTGGTTACAGAGCGCAGAGGAATACCTGGAGCTCAGTTCGTAGAAGATGTGCAGACCTATCTCACTCAAACTGGCTTCGACTGTAACTCTGCCCTCGCTTTTCTCCAAGAAAGACTTCAGCAATACAAAATGGTTGAAATGAAGCTTCAAGCTCAACAAAGGGAACTTCTGGCAAAGATTCCTGACATTGAGAAGTGCTTAGATGTTGTTGCCACTTTGCAAGCTAAGAAGGGTACTGGTGAGGCACTTATTACTGATTTTGAGATCTGTGAAGGGATATATTCACGAGCTAGCATTGAGGACACTGATTCAGTATGTTTATGGCTGGGAGCAAATGTCATGCTGGAGTATTCATGTGAAGAGGCTACTGCTCTTCTACGAAAGAATTTAGATAATGCTAAAGCCAGTTTGGAAGTTCTTCTTGCTGATCTACAGTTCTTGAGAGATCAAGTAACAATTACCCAGGTAACAATTGCTCGGGTGTACAACTGGGATGTTCATCAGCGCAGACTCCGACAAGCTTCCCCTTCTGCCATGGAATCTTGA

Protein Analysis

191

Amino Acids

21.3

Weight (kDa)

4.76

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 52 - 173 2.2e-30 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49510 AT5G49510
fragaria_vesca FvH4_4g20250
malus_domestica MD13G1150800.v1.1 MD16G1151300.v1.1
prunus_persica Prupe.1G115000_v2.0.a1
pyrus_communis pycom13g12960 pycom16g12930
rosa_chinensis RchiOBHm_Chr4g0425791
rosa_multiflora Rmu_sc0002094.1_g000001 Rmu_sc0010899.1_g000002
rosa_roxburghii Rroxscaffold_5G00367780
rosa_rugosa Rorug04G0206200
rosa_samantha Rh4AG263400 Rh4BG269300
rosa_wichuraiana Rw4G022730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 412
AcuI CTGAAG 1 cut(s) 128
AfaI GTAC 2 cut(s) 262, 512
AgsI TTSAA 3 cut(s) 21, 164, 176
AjiI CACGTC 1 cut(s) 9
AjnI CCWGG 2 cut(s) 52, 489
AjuI GAANNNNNNNTTGG 2 cut(s) 126, 158
AluBI AGCT 6 cut(s) 59, 172, 179, 251, 317, 548
AluI AGCT 6 cut(s) 59, 172, 179, 251, 317, 548
Alw21I GWGCWC 1 cut(s) 61
Ama87I CYCGRG 1 cut(s) 504
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 412
Asp700I GAANNNNTTC 2 cut(s) 141, 444
AspLEI GCGC 2 cut(s) 42, 534
AsuHPI GGTGA 1 cut(s) 278
AsuNHI GCTAGC 1 cut(s) 317
AvaI CYCGRG 1 cut(s) 504
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 61
BauI CACGAG 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 61
BciT130I CCWGG 2 cut(s) 54, 491
BfaI CTAG 1 cut(s) 318
BfmI CTRYAG 1 cut(s) 458
BglII AGATCT 1 cut(s) 291
Bme1390I CCNGG 2 cut(s) 54, 491
BmeT110I CYCGRG 1 cut(s) 504
BmgBI CACGTC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 54, 491
BmrI ACTGGG 1 cut(s) 526
BmtI GCTAGC 1 cut(s) 321
BmuI ACTGGG 1 cut(s) 526
BpmI CTGGAG 2 cut(s) 75, 392
BpuEI CTTGAG 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 525
BsaJI CCNNGG 3 cut(s) 24, 489, 561
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bse1I ACTGG 4 cut(s) 103, 268, 432, 521
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 2 cut(s) 54, 491
BseDI CCNNGG 3 cut(s) 24, 489, 561
BseGI GGATG 1 cut(s) 526
BseJI GATNNNNATC 1 cut(s) 525
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 4 cut(s) 103, 268, 432, 521
BseYI CCCAGC 1 cut(s) 352
BsgI GTGCAG 1 cut(s) 98
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 504
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 61
Bsp1407I TGTACA 1 cut(s) 510
Bsp143I GATC 3 cut(s) 291, 454, 472
Bsp19I CCATGG 1 cut(s) 561
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 73
BspOI GCTAGC 1 cut(s) 321
BspQI GCTCTTC 1 cut(s) 405
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 4 cut(s) 103, 268, 432, 521
BssECI CCNNGG 3 cut(s) 24, 489, 561
BssMI GATC 3 cut(s) 291, 454, 472
BssSI CACGAG 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 312
Bst2UI CCWGG 2 cut(s) 54, 491
Bst4CI ACNGT 2 cut(s) 110, 462
Bst6I CTCTTC 2 cut(s) 381, 405
BstAUI TGTACA 1 cut(s) 510
BstC8I GCNNGC 2 cut(s) 249, 319
BstDEI CTNAG 3 cut(s) 60, 226, 252
BstDSI CCRYGG 1 cut(s) 561
BstF5I GGATG 1 cut(s) 526
BstHHI GCGC 2 cut(s) 42, 534
BstKTI GATC 3 cut(s) 294, 457, 475
BstMBI GATC 3 cut(s) 291, 454, 472
BstNI CCWGG 2 cut(s) 54, 491
BstSCI CCNGG 2 cut(s) 52, 489
BstSFI CTRYAG 1 cut(s) 458
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 561
BtrI CACGTC 1 cut(s) 9
BtsCI GGATG 1 cut(s) 526
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 2 cut(s) 249, 319
CfoI GCGC 2 cut(s) 42, 534
Csp6I GTAC 2 cut(s) 261, 511
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 3 cut(s) 367, 381, 562
CviQI GTAC 2 cut(s) 261, 511
DdeI CTNAG 3 cut(s) 60, 226, 252
DpnI GATC 3 cut(s) 293, 456, 474
DpnII GATC 3 cut(s) 291, 454, 472
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 381, 405
EarI CTCTTC 2 cut(s) 381, 405
Ecl136II GAGCTC 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 504
EcoICRI GAGCTC 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 52, 489
EcoT14I CCWWGG 1 cut(s) 561
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 3 cut(s) 370, 384, 565
FaiI YATR 6 cut(s) 307, 343, 349, 368, 382, 563
FalI AAGNNNNNCTT 4 cut(s) 126, 158, 432, 464
FatI CATG 3 cut(s) 366, 380, 561
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 61
FspBI CTAG 1 cut(s) 318
GlaI GCGC 2 cut(s) 41, 533
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 2 cut(s) 75, 392
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 2 cut(s) 42, 534
Hin1II CATG 3 cut(s) 370, 384, 565
Hin6I GCGC 2 cut(s) 40, 532
HinP1I GCGC 2 cut(s) 40, 532
HindIII AAGCTT 2 cut(s) 170, 546
HinfI GANTC 4 cut(s) 205, 335, 537, 566
HphI GGTGA 1 cut(s) 278
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 1 cut(s) 542
Hpy188III TCNNGA 4 cut(s) 209, 312, 466, 570
Hpy8I GTNNAC 1 cut(s) 511
HpyAV CCTTC 3 cut(s) 250, 293, 564
HpyCH4III ACNGT 2 cut(s) 110, 462
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 2 cut(s) 79, 247
HpyF3I CTNAG 3 cut(s) 60, 226, 252
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 3 cut(s) 370, 384, 565
HspAI GCGC 2 cut(s) 40, 532
Kzo9I GATC 3 cut(s) 291, 454, 472
LguI GCTCTTC 1 cut(s) 405
LmnI GCTCC 2 cut(s) 56, 356
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 4 cut(s) 31, 110, 478, 493
MalI GATC 3 cut(s) 293, 456, 474
MboI GATC 3 cut(s) 291, 454, 472
MboII GAAGA 5 cut(s) 9, 83, 392, 398, 437
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 61
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 412, 483, 498
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 531
MmeI TCCRAC 1 cut(s) 565
MnlI CCTC 6 cut(s) 19, 38, 131, 262, 319, 382
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 141, 444
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 2 cut(s) 54, 491
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 2 cut(s) 54, 491
NcoI CCATGG 1 cut(s) 561
NdeII GATC 3 cut(s) 291, 454, 472
NheI GCTAGC 1 cut(s) 317
NlaIII CATG 3 cut(s) 370, 384, 565
PciSI GCTCTTC 1 cut(s) 405
PdmI GAANNNNTTC 2 cut(s) 141, 444
PfeI GAWTC 3 cut(s) 205, 335, 566
PleI GAGTC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 61
Psp6I CCWGG 2 cut(s) 52, 489
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 2 cut(s) 52, 489
PsuI RGATCY 1 cut(s) 291
RsaI GTAC 2 cut(s) 262, 512
RsaNI GTAC 2 cut(s) 261, 511
SacI GAGCTC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 405
Sau3AI GATC 3 cut(s) 291, 454, 472
SchI GAGTC 1 cut(s) 531
ScrFI CCNGG 2 cut(s) 54, 491
SduI GDGCHC 1 cut(s) 61
SfcI CTRYAG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 466
SmoI CTYRAG 1 cut(s) 466
Sse9I AATT 3 cut(s) 412, 483, 498
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 61
StyD4I CCNGG 2 cut(s) 52, 489
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 2 cut(s) 110, 462
TaiI ACGT 1 cut(s) 11
TaqI TCGA 1 cut(s) 105
TasI AATT 3 cut(s) 412, 483, 498
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 3 cut(s) 205, 335, 566
TscAI CASTG 1 cut(s) 337
TspDTI ATGAA 3 cut(s) 182, 369, 515
TspRI CASTG 1 cut(s) 337
XapI RAATTY 1 cut(s) 412
XmnI GAANNNNTTC 2 cut(s) 141, 444
XspI CTAG 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.