Rroxscaffold_5G00367780

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015096
Physical Location & Seq
Forward (+)
49639094 .. 49642445
3352 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00367780.1

Sequence Viewer

Length: 636 bp
ATGGCTACGTCGTCGTCGTCGTCGTCGGCAGAGGTGGTAACAGAGCGAAGAGGAATACCTGGAGCTCAGTTCGTAGAAGATGTGCAGACCTATCTCACCCAGACCGGCTTCGACTGTAACTCTGCCCTCGCTTTTCTCCAAGAAAGACTTCAGCAATACAAAATGGTTGAAATGAAACTTCAAGCTCAACAAAGGGAGCTTCTGTGTACTGACGCGGTTGCATCTGACATAGAGTTACAGCTTCTTGCTGATACTTACCAAGCAAAGATTCCTGACATTGAGAAGTGCTTAGATGTTGTTGCCACGTTGCAAGCTAAGAAGGGTACTGGTGAGGCACTTATTACTGATTTTGAGATCTCTGAAGGGATATATTCACGAGCTAGCATTGAGGATACTGATTCAGTATGTTTATGGCTGGGAGCTAATGTCATGTTGGAGTATTCTTGTGAAGAGGCTACTGCCCTTCTACGAAAGAATTTAGATAATGCTAAAGCCAGTTTAGAAGTTCTTCTTGCTGATCTACAGTTCTTGAGAGATCAAGTAACAATTACCCAGGTAACAATTGCTCGGGTGTACAACTGGGACGTTCATCAGCGCAGACTCCGACAAGCTTCCCCTTCTGCTAAGGACTCCTGA

Protein Analysis

211

Amino Acids

23.47

Weight (kDa)

4.6

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 84 - 194 1.8e-27 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49510 AT5G49510
fragaria_vesca FvH4_4g20250
malus_domestica MD13G1150800.v1.1 MD16G1151300.v1.1
prunus_persica Prupe.1G115000_v2.0.a1
pyrus_communis pycom13g12960 pycom16g12930
rosa_chinensis RchiOBHm_Chr4g0425791
rosa_multiflora Rmu_sc0002094.1_g000001 Rmu_sc0010899.1_g000002
rosa_roxburghii Rroxscaffold_5G00367780
rosa_rugosa Rorug04G0206200
rosa_samantha Rh4AG263400 Rh4BG269300
rosa_wichuraiana Rw4G022730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 215
AciI CCGC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 2 cut(s) 134, 381
AfaI GTAC 3 cut(s) 208, 325, 575
AgsI TTSAA 2 cut(s) 170, 182
AjnI CCWGG 2 cut(s) 58, 552
AjuI GAANNNNNNNTTGG 4 cut(s) 132, 164, 252, 284
AluBI AGCT 8 cut(s) 65, 185, 199, 241, 314, 380, 422, 611
AluI AGCT 8 cut(s) 65, 185, 199, 241, 314, 380, 422, 611
Alw21I GWGCWC 1 cut(s) 67
Ama87I CYCGRG 1 cut(s) 567
ApoI RAATTY 1 cut(s) 475
Asp700I GAANNNNTTC 2 cut(s) 147, 507
AspLEI GCGC 1 cut(s) 597
AsuHPI GGTGA 2 cut(s) 88, 341
AsuNHI GCTAGC 1 cut(s) 380
AvaI CYCGRG 1 cut(s) 567
BanII GRGCYC 1 cut(s) 67
BauI CACGAG 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 67
BciT130I CCWGG 2 cut(s) 60, 554
BciVI GTATCC 1 cut(s) 385
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 521
BfuI GTATCC 1 cut(s) 385
BglII AGATCT 1 cut(s) 354
Bme1390I CCNGG 2 cut(s) 60, 554
BmeT110I CYCGRG 1 cut(s) 567
BmrFI CCNGG 2 cut(s) 60, 554
BmrI ACTGGG 1 cut(s) 589
BmsI GCATC 1 cut(s) 230
BmtI GCTAGC 1 cut(s) 384
BmuI ACTGGG 1 cut(s) 589
BpmI CTGGAG 1 cut(s) 81
Bpu10I CCTNAGC 1 cut(s) 624
BpuEI CTTGAG 1 cut(s) 550
BsaJI CCNNGG 1 cut(s) 552
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 411, 441
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 3 cut(s) 331, 495, 584
BseBI CCWGG 2 cut(s) 60, 554
BseDI CCNNGG 1 cut(s) 552
BseMII CTCAG 1 cut(s) 80
BseNI ACTGG 3 cut(s) 331, 495, 584
BseYI CCCAGC 1 cut(s) 415
BsgI GTGCAG 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 567
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 596
BsoBI CYCGRG 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 67
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 3 cut(s) 354, 517, 535
BspACI CCGC 1 cut(s) 215
BspCNI CTCAG 1 cut(s) 79
BspFNI CGCG 1 cut(s) 215
BspOI GCTAGC 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 104
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 3 cut(s) 331, 495, 584
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 552
BssMI GATC 3 cut(s) 354, 517, 535
BssSI CACGAG 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 2 cut(s) 60, 554
Bst4CI ACNGT 2 cut(s) 116, 525
Bst6I CTCTTC 2 cut(s) 43, 444
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 2 cut(s) 312, 382
BstDEI CTNAG 4 cut(s) 66, 289, 315, 624
BstFNI CGCG 1 cut(s) 215
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 3 cut(s) 357, 520, 538
BstMBI GATC 3 cut(s) 354, 517, 535
BstNI CCWGG 2 cut(s) 60, 554
BstSCI CCNGG 2 cut(s) 58, 552
BstSFI CTRYAG 1 cut(s) 521
BstUI CGCG 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BsuI GTATCC 1 cut(s) 385
Cac8I GCNNGC 2 cut(s) 312, 382
CfoI GCGC 1 cut(s) 597
Cfr10I RCCGGY 1 cut(s) 104
CseI GACGC 1 cut(s) 221
Csp6I GTAC 3 cut(s) 207, 324, 574
CviAII CATG 1 cut(s) 430
CviQI GTAC 3 cut(s) 207, 324, 574
DdeI CTNAG 4 cut(s) 66, 289, 315, 624
DpnI GATC 3 cut(s) 356, 519, 537
DpnII GATC 3 cut(s) 354, 517, 535
Eam1104I CTCTTC 2 cut(s) 43, 444
EarI CTCTTC 2 cut(s) 43, 444
Ecl136II GAGCTC 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 65
Eco57I CTGAAG 2 cut(s) 134, 381
Eco88I CYCGRG 1 cut(s) 567
EcoICRI GAGCTC 1 cut(s) 65
EcoRII CCWGG 2 cut(s) 58, 552
EcoT38I GRGCYC 1 cut(s) 67
FaeI CATG 1 cut(s) 433
FaiI YATR 5 cut(s) 230, 370, 406, 412, 431
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 495, 527
FaqI GGGAC 1 cut(s) 596
FatI CATG 1 cut(s) 429
FriOI GRGCYC 1 cut(s) 67
FspBI CTAG 1 cut(s) 381
GlaI GCGC 1 cut(s) 596
GsaI CCCAGC 1 cut(s) 419
GsuI CTGGAG 1 cut(s) 81
HapII CCGG 1 cut(s) 105
HgaI GACGC 1 cut(s) 221
HhaI GCGC 1 cut(s) 597
Hin1II CATG 1 cut(s) 433
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 4 cut(s) 268, 398, 600, 629
HpaII CCGG 1 cut(s) 105
HphI GGTGA 2 cut(s) 88, 341
Hpy166II GTNNAC 2 cut(s) 207, 574
Hpy188I TCNGA 3 cut(s) 226, 361, 605
Hpy188III TCNNGA 4 cut(s) 272, 375, 529, 633
Hpy8I GTNNAC 2 cut(s) 207, 574
Hpy99I CGWCG 6 cut(s) 13, 16, 19, 22, 25, 28
HpyAV CCTTC 4 cut(s) 313, 356, 473, 627
HpyCH4III ACNGT 2 cut(s) 116, 525
HpyCH4IV ACGT 3 cut(s) 8, 305, 585
HpyCH4V TGCA 3 cut(s) 85, 221, 310
HpyF3I CTNAG 4 cut(s) 66, 289, 315, 624
HpySE526I ACGT 3 cut(s) 8, 305, 585
Hsp92II CATG 1 cut(s) 433
HspAI GCGC 1 cut(s) 595
Kzo9I GATC 3 cut(s) 354, 517, 535
LmnI GCTCC 3 cut(s) 62, 196, 419
LweI GCATC 1 cut(s) 230
MaeI CTAG 1 cut(s) 381
MaeII ACGT 3 cut(s) 8, 305, 585
MaeIII GTNAC 5 cut(s) 37, 116, 234, 541, 556
MalI GATC 3 cut(s) 356, 519, 537
MboI GATC 3 cut(s) 354, 517, 535
MboII GAAGA 4 cut(s) 60, 89, 461, 500
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 3 cut(s) 475, 546, 561
MlyI GAGTC 2 cut(s) 594, 623
MmeI TCCRAC 2 cut(s) 414, 628
MnlI CCTC 6 cut(s) 25, 44, 137, 325, 382, 445
MroXI GAANNNNTTC 2 cut(s) 147, 507
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 2 cut(s) 60, 554
MunI CAATTG 1 cut(s) 561
MvaI CCWGG 2 cut(s) 60, 554
MvnI CGCG 1 cut(s) 215
NdeII GATC 3 cut(s) 354, 517, 535
NheI GCTAGC 1 cut(s) 380
NlaIII CATG 1 cut(s) 433
PcsI WCGNNNNNNNCGW 3 cut(s) 14, 17, 20
PdmI GAANNNNTTC 2 cut(s) 147, 507
PfeI GAWTC 2 cut(s) 268, 398
PleI GAGTC 2 cut(s) 594, 623
PpsI GAGTC 2 cut(s) 594, 623
Psp124BI GAGCTC 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 58, 552
PspFI CCCAGC 1 cut(s) 415
PspGI CCWGG 2 cut(s) 58, 552
PsuI RGATCY 1 cut(s) 354
RsaI GTAC 3 cut(s) 208, 325, 575
RsaNI GTAC 3 cut(s) 207, 324, 574
SacI GAGCTC 1 cut(s) 67
Sau3AI GATC 3 cut(s) 354, 517, 535
SchI GAGTC 2 cut(s) 594, 623
ScrFI CCNGG 2 cut(s) 60, 554
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 1 cut(s) 230
SfcI CTRYAG 1 cut(s) 521
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
Sse9I AATT 3 cut(s) 475, 546, 561
SsiI CCGC 1 cut(s) 215
SspMI CTAG 1 cut(s) 381
SstI GAGCTC 1 cut(s) 67
StyD4I CCNGG 2 cut(s) 58, 552
TaaI ACNGT 2 cut(s) 116, 525
TaiI ACGT 3 cut(s) 11, 308, 588
TaqI TCGA 1 cut(s) 111
TasI AATT 3 cut(s) 475, 546, 561
TatI WGTACW 2 cut(s) 206, 573
TfiI GAWTC 2 cut(s) 268, 398
TspDTI ATGAA 2 cut(s) 188, 578
XapI RAATTY 1 cut(s) 475
XmnI GAANNNNTTC 2 cut(s) 147, 507
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.