Rh4BG269300

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015096
Physical Location & Seq
Forward (+)
44340310 .. 44343152
2843 bp
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UTR
Exon/CDS
Intron
Rh4BG269300.1

Sequence Viewer

Length: 582 bp
ATGGCTACGTCGTCGTCGTCGTCGTCGTCGGCAGAGGTGGTAACAGAGCGAAGAGGAATACCAGGAGCTCAGTTCGTAGAAGATGTGCAGACCTATCTCACCCAGACCGGCTTCGACTGTAACTCTGCCCTCGCTTTTCTCCAAGAAAGACTTCAGCAATACAAAATGGTTGAAATGAAACTTCAAGCTCAACAAAGGGAGCTTCTGGCAAAGATTCCTGACATTGAGAAGTGCTTAGATGTTGTTGCCACGTTGCAAGCTAAGAAGGGTACTGGTGAGGCACTTATTACTGATTTTGAGATCTCTGAAGGGATATATTCACGAGCTAGCATTGAGGATACTGATTCAGTATGTTTATGGCTGGGAGCTAATGTCATGTTGGAGTATTCTTGTGAAGAGGCTACTGCCCTTCTACGAAAGAATTTAGATAATGCTAAAGCCAGTTTAGAAGTTCTTCTTGCTGATCTACAGTTCTTGAGAGATCAAGTGACAATTACCCAGGTAACAATTGCTCGGGTGTACAACTGGGACGTTCATCAGCGCAGACTCCGACAAGCTTCCCCTTCTGCTAAGGACTCCTGA

Protein Analysis

193

Amino Acids

21.5

Weight (kDa)

4.84

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 55 - 176 2.7e-30 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49510 AT5G49510
fragaria_vesca FvH4_4g20250
malus_domestica MD13G1150800.v1.1 MD16G1151300.v1.1
prunus_persica Prupe.1G115000_v2.0.a1
pyrus_communis pycom13g12960 pycom16g12930
rosa_chinensis RchiOBHm_Chr4g0425791
rosa_multiflora Rmu_sc0002094.1_g000001 Rmu_sc0010899.1_g000002
rosa_roxburghii Rroxscaffold_5G00367780
rosa_rugosa Rorug04G0206200
rosa_samantha Rh4AG263400 Rh4BG269300
rosa_wichuraiana Rw4G022730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 421
AcuI CTGAAG 2 cut(s) 137, 327
AfaI GTAC 2 cut(s) 271, 521
AgsI TTSAA 2 cut(s) 173, 185
AjnI CCWGG 2 cut(s) 61, 498
AjuI GAANNNNNNNTTGG 2 cut(s) 135, 167
AluBI AGCT 7 cut(s) 68, 188, 202, 260, 326, 368, 557
AluI AGCT 7 cut(s) 68, 188, 202, 260, 326, 368, 557
Alw21I GWGCWC 1 cut(s) 70
Ama87I CYCGRG 1 cut(s) 513
ApoI RAATTY 1 cut(s) 421
Asp700I GAANNNNTTC 2 cut(s) 150, 453
AspLEI GCGC 1 cut(s) 543
AsuHPI GGTGA 2 cut(s) 91, 287
AsuNHI GCTAGC 1 cut(s) 326
AvaI CYCGRG 1 cut(s) 513
BanII GRGCYC 1 cut(s) 70
BauI CACGAG 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 70
BciT130I CCWGG 2 cut(s) 63, 500
BciVI GTATCC 1 cut(s) 331
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 1 cut(s) 467
BfuI GTATCC 1 cut(s) 331
BglII AGATCT 1 cut(s) 300
Bme1390I CCNGG 2 cut(s) 63, 500
BmeT110I CYCGRG 1 cut(s) 513
BmrFI CCNGG 2 cut(s) 63, 500
BmrI ACTGGG 1 cut(s) 535
BmtI GCTAGC 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 535
Bpu10I CCTNAGC 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 496
BsaJI CCNNGG 1 cut(s) 498
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 3 cut(s) 277, 441, 530
BseBI CCWGG 2 cut(s) 63, 500
BseDI CCNNGG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 3 cut(s) 277, 441, 530
BseYI CCCAGC 1 cut(s) 361
BsgI GTGCAG 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 542
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 70
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 3 cut(s) 300, 463, 481
BspCNI CTCAG 1 cut(s) 82
BspOI GCTAGC 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 107
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 3 cut(s) 277, 441, 530
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 3 cut(s) 300, 463, 481
BssSI CACGAG 1 cut(s) 321
Bst2BI CACGAG 1 cut(s) 321
Bst2UI CCWGG 2 cut(s) 63, 500
Bst4CI ACNGT 2 cut(s) 119, 471
Bst6I CTCTTC 2 cut(s) 46, 390
BstAUI TGTACA 1 cut(s) 519
BstC8I GCNNGC 2 cut(s) 258, 328
BstDEI CTNAG 4 cut(s) 69, 235, 261, 570
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 3 cut(s) 303, 466, 484
BstMBI GATC 3 cut(s) 300, 463, 481
BstNI CCWGG 2 cut(s) 63, 500
BstSCI CCNGG 2 cut(s) 61, 498
BstSFI CTRYAG 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BsuI GTATCC 1 cut(s) 331
Cac8I GCNNGC 2 cut(s) 258, 328
CfoI GCGC 1 cut(s) 543
Cfr10I RCCGGY 1 cut(s) 107
Csp6I GTAC 2 cut(s) 270, 520
CviAII CATG 1 cut(s) 376
CviQI GTAC 2 cut(s) 270, 520
DdeI CTNAG 4 cut(s) 69, 235, 261, 570
DpnI GATC 3 cut(s) 302, 465, 483
DpnII GATC 3 cut(s) 300, 463, 481
Eam1104I CTCTTC 2 cut(s) 46, 390
EarI CTCTTC 2 cut(s) 46, 390
Ecl136II GAGCTC 1 cut(s) 68
Eco24I GRGCYC 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 137, 327
Eco88I CYCGRG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 61, 498
EcoT38I GRGCYC 1 cut(s) 70
FaeI CATG 1 cut(s) 379
FaiI YATR 4 cut(s) 316, 352, 358, 377
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 441, 473
FaqI GGGAC 1 cut(s) 542
FatI CATG 1 cut(s) 375
FriOI GRGCYC 1 cut(s) 70
FspBI CTAG 1 cut(s) 327
GlaI GCGC 1 cut(s) 542
GsaI CCCAGC 1 cut(s) 365
HapII CCGG 1 cut(s) 108
HhaI GCGC 1 cut(s) 543
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 4 cut(s) 214, 344, 546, 575
HpaII CCGG 1 cut(s) 108
HphI GGTGA 2 cut(s) 91, 287
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 2 cut(s) 307, 551
Hpy188III TCNNGA 4 cut(s) 218, 321, 475, 579
Hpy8I GTNNAC 1 cut(s) 520
Hpy99I CGWCG 7 cut(s) 13, 16, 19, 22, 25, 28, 31
HpyAV CCTTC 4 cut(s) 259, 302, 419, 573
HpyCH4III ACNGT 2 cut(s) 119, 471
HpyCH4IV ACGT 3 cut(s) 8, 251, 531
HpyCH4V TGCA 2 cut(s) 88, 256
HpyF3I CTNAG 4 cut(s) 69, 235, 261, 570
HpySE526I ACGT 3 cut(s) 8, 251, 531
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 1 cut(s) 541
Kzo9I GATC 3 cut(s) 300, 463, 481
LmnI GCTCC 3 cut(s) 65, 199, 365
MaeI CTAG 1 cut(s) 327
MaeII ACGT 3 cut(s) 8, 251, 531
MaeIII GTNAC 4 cut(s) 40, 119, 487, 502
MalI GATC 3 cut(s) 302, 465, 483
MboI GATC 3 cut(s) 300, 463, 481
MboII GAAGA 4 cut(s) 63, 92, 407, 446
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 3 cut(s) 421, 492, 507
MlyI GAGTC 2 cut(s) 540, 569
MmeI TCCRAC 2 cut(s) 360, 574
MnlI CCTC 6 cut(s) 28, 47, 140, 271, 328, 391
MroXI GAANNNNTTC 2 cut(s) 150, 453
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 2 cut(s) 63, 500
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 2 cut(s) 63, 500
NdeII GATC 3 cut(s) 300, 463, 481
NheI GCTAGC 1 cut(s) 326
NlaIII CATG 1 cut(s) 379
NmuCI GTSAC 1 cut(s) 487
PcsI WCGNNNNNNNCGW 4 cut(s) 14, 17, 20, 23
PdmI GAANNNNTTC 2 cut(s) 150, 453
PfeI GAWTC 2 cut(s) 214, 344
PleI GAGTC 2 cut(s) 540, 569
PpsI GAGTC 2 cut(s) 540, 569
Psp124BI GAGCTC 1 cut(s) 70
Psp6I CCWGG 2 cut(s) 61, 498
PspFI CCCAGC 1 cut(s) 361
PspGI CCWGG 2 cut(s) 61, 498
PsuI RGATCY 1 cut(s) 300
RsaI GTAC 2 cut(s) 271, 521
RsaNI GTAC 2 cut(s) 270, 520
SacI GAGCTC 1 cut(s) 70
Sau3AI GATC 3 cut(s) 300, 463, 481
SchI GAGTC 2 cut(s) 540, 569
ScrFI CCNGG 2 cut(s) 63, 500
SduI GDGCHC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 3 cut(s) 421, 492, 507
SspMI CTAG 1 cut(s) 327
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 2 cut(s) 61, 498
TaaI ACNGT 2 cut(s) 119, 471
TaiI ACGT 3 cut(s) 11, 254, 534
TaqI TCGA 1 cut(s) 114
TasI AATT 3 cut(s) 421, 492, 507
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 2 cut(s) 214, 344
TseFI GTSAC 1 cut(s) 487
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 2 cut(s) 191, 524
XapI RAATTY 1 cut(s) 421
XmnI GAANNNNTTC 2 cut(s) 150, 453
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.