MD13G1150800.v1.1

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015096
Physical Location & Seq
Reverse (-)
11825627 .. 11828627
3001 bp
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UTR
Exon/CDS
Intron
MD13G1150800.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGCTACGGAATCGCCATCATCGTCGTCTGCGGCGGTGACAGAGCGGAGAGGAATACCAGGAGCGCAGTTCGTAGAGGACGTGCAGACCTATCTCACCCAGTTAGGCCTCGACGTCAACTCTGCCCTCGCCTTTCTCCAAGAAAGACTTCAGCAGTACAAATTGGTTGAAATGAAGCTTCAAGCTCAGCAGAGAGATCTTCAGGCAAAGATCCCTGATATTGAGAAGTGCTTAGACGTTGTTGCCACGTTGCAAGCCAAGAAGGGTACTGGTGAGGAACTTATTACAGATTTTGAGGTATCTGAAGGGATATATTCACGAGCTAGCATTGACGATACTGAGTCAGTCTGTTTATGGCTGGGTGCAAATGTCATGTTGGAGTATTCTTGTGAAGAGGCTACTGCTCTTCTCCGAAAGAATTTAGACAATGCTAGAGCCAGTTTAGAAGTTCTTCTTGCTGATCTACAATTCTTGAGAGATCAAGTGACAATAACCCAGGTAACAATTGCTCGGGTGTACAACTGGGATGTTCATCAGCGCAGACTTCGACAATCTTCCCCTAAAGATTCGTGA

Protein Analysis

191

Amino Acids

21.28

Weight (kDa)

4.76

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 53 - 175 1.9e-30 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015096)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49510 AT5G49510
fragaria_vesca FvH4_4g20250
malus_domestica MD13G1150800.v1.1 MD16G1151300.v1.1
prunus_persica Prupe.1G115000_v2.0.a1
pyrus_communis pycom13g12960 pycom16g12930
rosa_chinensis RchiOBHm_Chr4g0425791
rosa_multiflora Rmu_sc0002094.1_g000001 Rmu_sc0010899.1_g000002
rosa_roxburghii Rroxscaffold_5G00367780
rosa_rugosa Rorug04G0206200
rosa_samantha Rh4AG263400 Rh4BG269300
rosa_wichuraiana Rw4G022730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
AccBSI CCGCTC 1 cut(s) 46
AciI CCGC 3 cut(s) 32, 35, 46
AclWI GGATC 1 cut(s) 205
AcsI RAATTY 1 cut(s) 418
AcuI CTGAAG 3 cut(s) 134, 185, 324
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 3 cut(s) 158, 268, 518
AgsI TTSAA 2 cut(s) 170, 182
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 2 cut(s) 58, 495
AjuI GAANNNNNNNTTGG 2 cut(s) 132, 164
AluBI AGCT 3 cut(s) 178, 185, 323
AluI AGCT 3 cut(s) 178, 185, 323
AlwI GGATC 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 1 cut(s) 106
ApoI RAATTY 1 cut(s) 418
Asp700I GAANNNNTTC 2 cut(s) 147, 450
AspLEI GCGC 2 cut(s) 67, 540
AsuHPI GGTGA 3 cut(s) 49, 88, 284
AsuNHI GCTAGC 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 510
BauI CACGAG 1 cut(s) 318
BccI CCATC 1 cut(s) 25
BciT130I CCWGG 2 cut(s) 60, 497
BfaI CTAG 2 cut(s) 324, 432
BglII AGATCT 1 cut(s) 196
BisI GCNGC 1 cut(s) 33
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 2 cut(s) 60, 497
BmeT110I CYCGRG 1 cut(s) 510
BmgBI CACGTC 1 cut(s) 82
BmrFI CCNGG 2 cut(s) 60, 497
BmrI ACTGGG 2 cut(s) 94, 532
BmtI GCTAGC 1 cut(s) 327
BmuI ACTGGG 2 cut(s) 94, 532
Bpu1102I GCTNAGC 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 493
BsaBI GATNNNNATC 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 495
Bse1I ACTGG 4 cut(s) 100, 274, 438, 527
Bse8I GATNNNNATC 1 cut(s) 531
BseBI CCWGG 2 cut(s) 60, 497
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 1 cut(s) 532
BseJI GATNNNNATC 1 cut(s) 531
BseMII CTCAG 2 cut(s) 200, 330
BseNI ACTGG 4 cut(s) 100, 274, 438, 527
BseYI CCCAGC 1 cut(s) 358
BsgI GTGCAG 1 cut(s) 104
BshFI GGCC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 510
BsnI GGCC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 510
Bsp1407I TGTACA 1 cut(s) 516
Bsp143I GATC 4 cut(s) 196, 210, 460, 478
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 3 cut(s) 32, 35, 46
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 2 cut(s) 199, 331
BspOI GCTAGC 1 cut(s) 327
BspPI GGATC 1 cut(s) 205
BspQI GCTCTTC 1 cut(s) 411
BsrBI CCGCTC 1 cut(s) 46
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 4 cut(s) 100, 274, 438, 527
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 4 cut(s) 196, 210, 460, 478
BssNI GRCGYC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 318
Bst2BI CACGAG 1 cut(s) 318
Bst2UI CCWGG 2 cut(s) 60, 497
Bst6I CTCTTC 2 cut(s) 387, 411
BstACI GRCGYC 1 cut(s) 114
BstAUI TGTACA 1 cut(s) 516
BstC8I GCNNGC 2 cut(s) 255, 325
BstDEI CTNAG 3 cut(s) 186, 232, 339
BstF5I GGATG 1 cut(s) 532
BstHHI GCGC 2 cut(s) 67, 540
BstKTI GATC 4 cut(s) 199, 213, 463, 481
BstMBI GATC 4 cut(s) 196, 210, 460, 478
BstNI CCWGG 2 cut(s) 60, 497
BstSCI CCNGG 2 cut(s) 58, 495
BstX2I RGATCY 2 cut(s) 196, 210
BstYI RGATCY 2 cut(s) 196, 210
BsuRI GGCC 1 cut(s) 108
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 532
Cac8I GCNNGC 2 cut(s) 255, 325
CfoI GCGC 2 cut(s) 67, 540
Csp6I GTAC 3 cut(s) 157, 267, 517
CviAII CATG 1 cut(s) 373
CviJI RGCY 9 cut(s) 5, 108, 178, 185, 257, 323, 358, 398, 437
CviKI_1 RGCY 9 cut(s) 5, 108, 178, 185, 257, 323, 358, 398, 437
CviQI GTAC 3 cut(s) 157, 267, 517
DdeI CTNAG 3 cut(s) 186, 232, 339
DpnI GATC 4 cut(s) 198, 212, 462, 480
DpnII GATC 4 cut(s) 196, 210, 460, 478
Eam1104I CTCTTC 2 cut(s) 387, 411
EarI CTCTTC 2 cut(s) 387, 411
Eco147I AGGCCT 1 cut(s) 108
Eco57I CTGAAG 3 cut(s) 134, 185, 324
Eco88I CYCGRG 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 58, 495
FaeI CATG 1 cut(s) 376
FaiI YATR 3 cut(s) 313, 355, 374
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 438, 470
FatI CATG 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 1 cut(s) 539
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 2 cut(s) 324, 432
GlaI GCGC 2 cut(s) 66, 539
GluI GCNGC 1 cut(s) 33
GsaI CCCAGC 1 cut(s) 362
HaeIII GGCC 1 cut(s) 108
HhaI GCGC 2 cut(s) 67, 540
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 1 cut(s) 376
Hin6I GCGC 2 cut(s) 65, 538
HinP1I GCGC 2 cut(s) 65, 538
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 3 cut(s) 11, 341, 566
HphI GGTGA 3 cut(s) 49, 88, 284
Hpy166II GTNNAC 2 cut(s) 118, 517
Hpy188I TCNGA 2 cut(s) 304, 413
Hpy188III TCNNGA 3 cut(s) 318, 472, 570
Hpy8I GTNNAC 2 cut(s) 118, 517
Hpy99I CGWCG 2 cut(s) 28, 116
HpyAV CCTTC 2 cut(s) 256, 299
HpyCH4IV ACGT 4 cut(s) 81, 114, 237, 248
HpyCH4V TGCA 3 cut(s) 85, 253, 365
HpyF3I CTNAG 3 cut(s) 186, 232, 339
HpySE526I ACGT 4 cut(s) 81, 114, 237, 248
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 1 cut(s) 376
HspAI GCGC 2 cut(s) 65, 538
Kzo9I GATC 4 cut(s) 196, 210, 460, 478
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 1 cut(s) 62
MaeI CTAG 2 cut(s) 324, 432
MaeII ACGT 4 cut(s) 81, 114, 237, 248
MaeIII GTNAC 3 cut(s) 37, 484, 499
MalI GATC 4 cut(s) 198, 212, 462, 480
MbiI CCGCTC 1 cut(s) 46
MboI GATC 4 cut(s) 196, 210, 460, 478
MboII GAAGA 5 cut(s) 191, 398, 404, 443, 546
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 2 cut(s) 196, 210
MluCI AATT 4 cut(s) 161, 418, 467, 504
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 357
MnlI CCTC 7 cut(s) 44, 70, 119, 137, 268, 289, 388
MroXI GAANNNNTTC 2 cut(s) 147, 450
MspR9I CCNGG 2 cut(s) 60, 497
MunI CAATTG 1 cut(s) 504
MvaI CCWGG 2 cut(s) 60, 497
NdeII GATC 4 cut(s) 196, 210, 460, 478
NheI GCTAGC 1 cut(s) 323
NlaIII CATG 1 cut(s) 376
NmuCI GTSAC 2 cut(s) 37, 484
PceI AGGCCT 1 cut(s) 108
PciSI GCTCTTC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 78
PdmI GAANNNNTTC 2 cut(s) 147, 450
PfeI GAWTC 2 cut(s) 11, 566
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 58, 495
PspFI CCCAGC 1 cut(s) 358
PspGI CCWGG 2 cut(s) 58, 495
PsuI RGATCY 2 cut(s) 196, 210
RsaI GTAC 3 cut(s) 158, 268, 518
RsaNI GTAC 3 cut(s) 157, 267, 517
SapI GCTCTTC 1 cut(s) 411
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 4 cut(s) 196, 210, 460, 478
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 2 cut(s) 60, 497
SmlI CTYRAG 1 cut(s) 472
SmoI CTYRAG 1 cut(s) 472
Sse9I AATT 4 cut(s) 161, 418, 467, 504
SseBI AGGCCT 1 cut(s) 108
SsiI CCGC 3 cut(s) 32, 35, 46
SspMI CTAG 2 cut(s) 324, 432
StuI AGGCCT 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 58, 495
TaiI ACGT 4 cut(s) 84, 117, 240, 251
TaqI TCGA 2 cut(s) 111, 547
TasI AATT 4 cut(s) 161, 418, 467, 504
TatI WGTACW 2 cut(s) 156, 516
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 2 cut(s) 11, 566
TseFI GTSAC 2 cut(s) 37, 484
Tsp45I GTSAC 2 cut(s) 37, 484
TspDTI ATGAA 2 cut(s) 188, 521
TspGWI ACGGA 1 cut(s) 23
XapI RAATTY 1 cut(s) 418
XmnI GAANNNNTTC 2 cut(s) 147, 450
XspI CTAG 2 cut(s) 324, 432
ZraI GACGTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.