FvH4_4g20350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011555
Physical Location & Seq
Reverse (-)
23659863 .. 23661801
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20350.t1

Sequence Viewer

Length: 627 bp
ATGATGAGAGAGTTCCAAACACCTCACAAGGACCATGCTCGACCTTATCCCAAGCTCTCCGCTCGCGTCTCCAAGAAGACCGTTCCGAAGAAGAAGAGCTTGAACTCTGCGTTTTCTTCAATCTCCGAAGATCTACTAGTCCCGCACTCGGTGGATGCCACTCCGATCTCTGTGATTTCTGAGATCGACGAGAGTCTCATGACCCTGCCAGATCCATCTCTCTCAGCTTCAACAGAAACCTTGTTTACCTCTGATCTCTCTCCATCTTCAGAGTCCACGATTTCAAGCCATGCCTCTTGGAGTGTTGCTAAATGTGAAAATGATGGCTCAAAGTTTGGTTCTATGGGGCCTGAGGTTGTAGCAAGATTTCTCAGGCAAGCTCGGACTCAGGTTCTAAACTCTGCTGGTGGGGATGCCAAATCCAAGAAGCTTCTGGATGCATTGGTTGAGATTGTGATAGAGGATTTCCACACTGTGCCTGAAGAGAGGGACCGGATTGCTGAACTTCTTTCAGCAAAAGCTATAATACTGGTTGTGTGTTCTTTGCTATGGATCCTTGCAATGGCTGTGTTTTTGGTGTTCAGCTCTGGAGCACAAAGCACTTTTCCTGGACCACTGCCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

22.5

Weight (kDa)

5.96

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 62
AccII CGCG 1 cut(s) 66
AciI CCGC 2 cut(s) 60, 143
AclWI GGATC 3 cut(s) 206, 547, 560
AcuI CTGAAG 2 cut(s) 252, 501
AdeI CACNNNGTG 2 cut(s) 151, 475
AfiI CCNNNNNNNGG 2 cut(s) 148, 562
AgsI TTSAA 4 cut(s) 103, 120, 231, 285
AhlI ACTAGT 1 cut(s) 136
AjnI CCWGG 1 cut(s) 607
AluBI AGCT 7 cut(s) 55, 99, 227, 380, 430, 521, 585
AluI AGCT 7 cut(s) 55, 99, 227, 380, 430, 521, 585
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 2 cut(s) 73, 200
AlwI GGATC 3 cut(s) 206, 547, 560
AoxI GGCC 1 cut(s) 347
AspS9I GGNCC 4 cut(s) 31, 347, 490, 611
AvaII GGWCC 3 cut(s) 31, 490, 611
AxyI CCTNAGG 1 cut(s) 351
BamHI GGATCC 1 cut(s) 552
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 595
BccI CCATC 3 cut(s) 223, 271, 317
BciT130I CCWGG 1 cut(s) 609
BcoDI GTCTC 2 cut(s) 73, 200
BcuI ACTAGT 1 cut(s) 136
BfaI CTAG 1 cut(s) 137
BglII AGATCT 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 609
Bme18I GGWCC 3 cut(s) 31, 490, 611
BmgT120I GGNCC 4 cut(s) 31, 347, 490, 611
BmiI GGNNCC 3 cut(s) 348, 491, 554
BmrFI CCNGG 1 cut(s) 609
BmsI GCATC 3 cut(s) 145, 403, 427
BoxI GACNNNNGTC 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 609
BsaWI WCCGGW 1 cut(s) 492
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 562
Bse1I ACTGG 1 cut(s) 534
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 1 cut(s) 609
BseGI GGATG 3 cut(s) 160, 418, 442
BseLI CCNNNNNNNGG 2 cut(s) 148, 562
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 5 cut(s) 171, 237, 342, 385, 401
BseNI ACTGG 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 1 cut(s) 493
BslFI GGGAC 2 cut(s) 125, 503
BslI CCNNNNNNNGG 2 cut(s) 148, 562
BsmAI GTCTC 2 cut(s) 73, 200
BsmBI CGTCTC 1 cut(s) 73
BsmFI GGGAC 2 cut(s) 125, 503
BsnI GGCC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 595
Bsp143I GATC 6 cut(s) 130, 165, 183, 211, 253, 552
BspACI CCGC 2 cut(s) 60, 143
BspANI GGCC 1 cut(s) 349
BspCNI CTCAG 5 cut(s) 172, 236, 343, 384, 400
BspFNI CGCG 1 cut(s) 66
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 348, 491, 554
BspPI GGATC 3 cut(s) 206, 547, 560
BspQI GCTCTTC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 567
BsrI ACTGG 1 cut(s) 534
BssMI GATC 6 cut(s) 130, 165, 183, 211, 253, 552
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 2 cut(s) 82, 475
Bst6I CTCTTC 2 cut(s) 89, 477
BstC8I GCNNGC 2 cut(s) 64, 378
BstDEI CTNAG 5 cut(s) 180, 223, 351, 371, 387
BstF5I GGATG 3 cut(s) 160, 418, 442
BstFNI CGCG 1 cut(s) 66
BstKTI GATC 6 cut(s) 133, 168, 186, 214, 256, 555
BstMAI GTCTC 2 cut(s) 73, 200
BstMBI GATC 6 cut(s) 130, 165, 183, 211, 253, 552
BstNI CCWGG 1 cut(s) 609
BstPAI GACNNNNGTC 1 cut(s) 192
BstSCI CCNGG 1 cut(s) 607
BstUI CGCG 1 cut(s) 66
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 3 cut(s) 130, 211, 552
BstYI RGATCY 3 cut(s) 130, 211, 552
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 1 cut(s) 349
BtsCI GGATG 3 cut(s) 160, 418, 442
BtsI GCAGTG 1 cut(s) 614
BtsIMutI CAGTG 2 cut(s) 471, 614
Cac8I GCNNGC 2 cut(s) 64, 378
CciI TCATGA 1 cut(s) 198
Cfr13I GGNCC 4 cut(s) 31, 347, 490, 611
CseI GACGC 1 cut(s) 55
CviAII CATG 3 cut(s) 35, 199, 290
DdeI CTNAG 5 cut(s) 180, 223, 351, 371, 387
DpnI GATC 6 cut(s) 132, 167, 185, 213, 255, 554
DpnII GATC 6 cut(s) 130, 165, 183, 211, 253, 552
DraIII CACNNNGTG 2 cut(s) 151, 475
Eam1104I CTCTTC 2 cut(s) 89, 477
EarI CTCTTC 2 cut(s) 89, 477
Eco47I GGWCC 3 cut(s) 31, 490, 611
Eco57I CTGAAG 2 cut(s) 252, 501
Eco81I CCTNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 607
EcoT22I ATGCAT 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 3 cut(s) 38, 202, 293
FaiI YATR 6 cut(s) 36, 200, 291, 344, 524, 550
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 2 cut(s) 125, 503
FatI CATG 3 cut(s) 34, 198, 289
FauI CCCGC 1 cut(s) 150
FokI GGATG 3 cut(s) 167, 425, 449
FspBI CTAG 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 609
HaeIII GGCC 1 cut(s) 349
HapII CCGG 1 cut(s) 493
HgaI GACGC 1 cut(s) 55
Hin1II CATG 3 cut(s) 38, 202, 293
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 3 cut(s) 193, 272, 385
HpaII CCGG 1 cut(s) 493
Hpy166II GTNNAC 2 cut(s) 246, 276
Hpy188I TCNGA 7 cut(s) 87, 127, 165, 181, 253, 271, 384
Hpy188III TCNNGA 3 cut(s) 199, 434, 588
Hpy8I GTNNAC 2 cut(s) 246, 276
Hpy99I CGWCG 1 cut(s) 191
HpyCH4III ACNGT 2 cut(s) 82, 475
HpyCH4V TGCA 2 cut(s) 440, 560
HpyF3I CTNAG 5 cut(s) 180, 223, 351, 371, 387
Hsp92II CATG 3 cut(s) 38, 202, 293
Kzo9I GATC 6 cut(s) 130, 165, 183, 211, 253, 552
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 1 cut(s) 590
LweI GCATC 3 cut(s) 145, 403, 427
MaeI CTAG 1 cut(s) 137
MalI GATC 6 cut(s) 132, 167, 185, 213, 255, 554
MbiI CCGCTC 1 cut(s) 62
MboI GATC 6 cut(s) 130, 165, 183, 211, 253, 552
MboII GAAGA 8 cut(s) 88, 100, 103, 106, 108, 140, 258, 494
MflI RGATCY 3 cut(s) 130, 211, 552
MhlI GDGCHC 1 cut(s) 595
MlyI GAGTC 3 cut(s) 202, 281, 379
MnlI CCTC 6 cut(s) 33, 259, 304, 346, 454, 480
Mph1103I ATGCAT 1 cut(s) 442
MspI CCGG 1 cut(s) 493
MspR9I CCNGG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 1 cut(s) 66
NdeII GATC 6 cut(s) 130, 165, 183, 211, 253, 552
NlaIII CATG 3 cut(s) 38, 202, 293
NlaIV GGNNCC 3 cut(s) 348, 491, 554
NsiI ATGCAT 1 cut(s) 442
PagI TCATGA 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 607
PleI GAGTC 3 cut(s) 201, 280, 379
PpsI GAGTC 3 cut(s) 201, 280, 379
PshAI GACNNNNGTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 3 cut(s) 348, 491, 554
PspPI GGNCC 4 cut(s) 31, 347, 490, 611
PsuI RGATCY 3 cut(s) 130, 211, 552
SapI GCTCTTC 1 cut(s) 89
Sau3AI GATC 6 cut(s) 130, 165, 183, 211, 253, 552
Sau96I GGNCC 4 cut(s) 31, 347, 490, 611
SchI GAGTC 3 cut(s) 202, 281, 379
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 1 cut(s) 595
SfaNI GCATC 3 cut(s) 145, 403, 427
SinI GGWCC 3 cut(s) 31, 490, 611
SpeI ACTAGT 1 cut(s) 136
SsiI CCGC 2 cut(s) 60, 143
SspMI CTAG 1 cut(s) 137
StyD4I CCNGG 1 cut(s) 607
TaaI ACNGT 2 cut(s) 82, 475
TaqI TCGA 2 cut(s) 40, 186
TscAI CASTG 2 cut(s) 478, 621
TspRI CASTG 2 cut(s) 478, 621
VpaK11BI GGWCC 3 cut(s) 31, 490, 611
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XspI CTAG 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.