MD13G1151000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011555
Physical Location & Seq
Reverse (-)
11879977 .. 11880739
763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1151000.v1.1.491

Sequence Viewer

Length: 459 bp
ATGTGGATTTCAACGAGTCTGATGCTGGTGCCGGATCCAGCTCTGTTGATGCCATCAGAGACATTGCTTCCATCCAATGTCTCACTGTCTCCGGAGGCCAGTGTTAATATGGACGGGCTGGGCAATGTCTCTCTGAGTAGTGGTAAATCAAGCGAATTTGATGGCTCAAACTTTGGTTCTATGGAGGCCGACATTGTAACGAGATTTCTCAGGCAAGCGCAGACTCAGGTCTTGAATTCCGAGGTGGATACTAAATGTAAGAAGATTCTGGATGCGCTGGTTGACTTTGTCTTAGAGGAGCTCCATACTTCACCTGCAGAGAGGGACCGGGTTACTGAACTTCTGTCGACAAAAGTTTACATAACAATGGCGTGTTTCGTGCTCTGGATCATTGCATTGTCTGTCTGGGCGTTCAGCTCTGGACACCGAAGCACCTTTCATGGACCTCTGCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.62

Weight (kDa)

4.78

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 322
Acc36I ACCTGC 1 cut(s) 322
AccB1I GGYRCC 1 cut(s) 28
AccI GTMKAC 1 cut(s) 347
AccIII TCCGGA 1 cut(s) 91
AclWI GGATC 3 cut(s) 29, 42, 395
AcsI RAATTY 2 cut(s) 155, 235
AgsI TTSAA 2 cut(s) 12, 235
AluBI AGCT 3 cut(s) 41, 301, 417
AluI AGCT 3 cut(s) 41, 301, 417
Alw21I GWGCWC 2 cut(s) 303, 384
Alw26I GTCTC 4 cut(s) 53, 85, 93, 133
AlwI GGATC 3 cut(s) 29, 42, 395
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 2 cut(s) 96, 186
ApoI RAATTY 2 cut(s) 155, 235
AspLEI GCGC 2 cut(s) 220, 277
AspS9I GGNCC 2 cut(s) 325, 443
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 303
AvaII GGWCC 2 cut(s) 325, 443
BamHI GGATCC 1 cut(s) 34
BanI GGYRCC 1 cut(s) 28
BanII GRGCYC 1 cut(s) 303
Bbv12I GWGCWC 2 cut(s) 303, 384
BccI CCATC 3 cut(s) 61, 79, 155
BcgI CGANNNNNNTGC 2 cut(s) 4, 38
BciVI GTATCC 1 cut(s) 241
BcnI CCSGG 1 cut(s) 329
BcoDI GTCTC 4 cut(s) 53, 85, 93, 133
BfmI CTRYAG 1 cut(s) 315
BfuAI ACCTGC 1 cut(s) 322
BfuI GTATCC 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 329
Bme18I GGWCC 2 cut(s) 325, 443
BmgT120I GGNCC 2 cut(s) 325, 443
BmiI GGNNCC 3 cut(s) 30, 36, 326
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 3 cut(s) 12, 39, 262
BoxI GACNNNNGTC 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 240
BsaWI WCCGGW 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 99
Bse3DI GCAATG 3 cut(s) 62, 130, 390
BseAI TCCGGA 1 cut(s) 91
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 2 cut(s) 71, 277
BseMI GCAATG 3 cut(s) 62, 130, 390
BseMII CTCAG 3 cut(s) 125, 223, 239
BseNI ACTGG 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 311
BseYI CCCAGC 1 cut(s) 118
BshFI GGCC 2 cut(s) 98, 188
BshNI GGYRCC 1 cut(s) 28
BsiHKAI GWGCWC 2 cut(s) 303, 384
BsiSI CCGG 3 cut(s) 32, 92, 328
BslFI GGGAC 1 cut(s) 338
BsmAI GTCTC 4 cut(s) 53, 85, 93, 133
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 2 cut(s) 98, 188
Bsp1286I GDGCHC 2 cut(s) 303, 384
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 2 cut(s) 34, 387
BspANI GGCC 2 cut(s) 98, 188
BspCNI CTCAG 3 cut(s) 126, 222, 238
BspEI TCCGGA 1 cut(s) 91
BspLI GGNNCC 3 cut(s) 30, 36, 326
BspMAI CTGCAG 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 3 cut(s) 29, 42, 395
BspT107I GGYRCC 1 cut(s) 28
BsrDI GCAATG 3 cut(s) 62, 130, 390
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 2 cut(s) 34, 387
Bst4CI ACNGT 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 4 cut(s) 134, 209, 225, 292
BstF5I GGATG 2 cut(s) 71, 277
BstHHI GCGC 2 cut(s) 220, 277
BstKTI GATC 2 cut(s) 37, 390
BstMAI GTCTC 4 cut(s) 53, 85, 93, 133
BstMBI GATC 2 cut(s) 34, 387
BstPAI GACNNNNGTC 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 327
BstSFI CTRYAG 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 2 cut(s) 98, 188
BtsCI GGATG 2 cut(s) 71, 277
BtsIMutI CAGTG 2 cut(s) 83, 106
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 2 cut(s) 220, 277
Cfr13I GGNCC 2 cut(s) 325, 443
CviAII CATG 1 cut(s) 440
CviJI RGCY 7 cut(s) 41, 98, 118, 165, 188, 301, 417
CviKI_1 RGCY 7 cut(s) 41, 98, 118, 165, 188, 301, 417
DdeI CTNAG 4 cut(s) 134, 209, 225, 292
DpnI GATC 2 cut(s) 36, 389
DpnII GATC 2 cut(s) 34, 387
Ecl136II GAGCTC 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 303
Eco47I GGWCC 2 cut(s) 325, 443
Eco53kI GAGCTC 1 cut(s) 301
EcoICRI GAGCTC 1 cut(s) 301
EcoRI GAATTC 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 303
FaeI CATG 1 cut(s) 443
FaiI YATR 5 cut(s) 110, 182, 306, 362, 441
FaqI GGGAC 1 cut(s) 338
FatI CATG 1 cut(s) 439
FblI GTMKAC 1 cut(s) 347
FokI GGATG 2 cut(s) 58, 284
FriOI GRGCYC 1 cut(s) 303
GlaI GCGC 2 cut(s) 219, 276
GsaI CCCAGC 1 cut(s) 122
HaeIII GGCC 2 cut(s) 98, 188
HapII CCGG 3 cut(s) 32, 92, 328
HhaI GCGC 2 cut(s) 220, 277
Hin1II CATG 1 cut(s) 443
Hin6I GCGC 2 cut(s) 218, 275
HinP1I GCGC 2 cut(s) 218, 275
HincII GTYRAC 2 cut(s) 283, 348
HindII GTYRAC 2 cut(s) 283, 348
HinfI GANTC 3 cut(s) 16, 223, 265
HpaII CCGG 3 cut(s) 32, 92, 328
HphI GGTGA 1 cut(s) 303
Hpy166II GTNNAC 3 cut(s) 283, 348, 358
Hpy188I TCNGA 4 cut(s) 21, 58, 135, 241
Hpy188III TCNNGA 5 cut(s) 92, 232, 269, 385, 420
Hpy8I GTNNAC 3 cut(s) 283, 348, 358
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 2 cut(s) 317, 395
HpyF3I CTNAG 4 cut(s) 134, 209, 225, 292
Hsp92II CATG 1 cut(s) 443
HspAI GCGC 2 cut(s) 218, 275
Kpn2I TCCGGA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 34, 387
LmnI GCTCC 2 cut(s) 298, 306
LweI GCATC 3 cut(s) 12, 39, 262
MaeIII GTNAC 2 cut(s) 196, 331
MalI GATC 2 cut(s) 36, 389
MboI GATC 2 cut(s) 34, 387
MboII GAAGA 1 cut(s) 274
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 2 cut(s) 303, 384
MluCI AATT 2 cut(s) 155, 235
MlyI GAGTC 2 cut(s) 25, 217
MnlI CCTC 6 cut(s) 88, 178, 235, 289, 315, 456
MroI TCCGGA 1 cut(s) 91
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 1 cut(s) 365
MspI CCGG 3 cut(s) 32, 92, 328
MspR9I CCNGG 1 cut(s) 329
NciI CCSGG 1 cut(s) 329
NdeII GATC 2 cut(s) 34, 387
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 3 cut(s) 30, 36, 326
PaqCI CACCTGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 265
PflFI GACNNNGTC 1 cut(s) 287
PleI GAGTC 2 cut(s) 24, 217
PpsI GAGTC 2 cut(s) 24, 217
PshAI GACNNNNGTC 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 303
PspFI CCCAGC 1 cut(s) 118
PspN4I GGNNCC 3 cut(s) 30, 36, 326
PspPI GGNCC 2 cut(s) 325, 443
PstI CTGCAG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 34
PsyI GACNNNGTC 1 cut(s) 287
RseI CAYNNNNRTG 1 cut(s) 365
SacI GAGCTC 1 cut(s) 303
SalI GTCGAC 1 cut(s) 346
SaqAI TTAA 1 cut(s) 105
Sau3AI GATC 2 cut(s) 34, 387
Sau96I GGNCC 2 cut(s) 325, 443
SchI GAGTC 2 cut(s) 25, 217
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 2 cut(s) 303, 384
SetI ASST 8 cut(s) 43, 231, 246, 303, 316, 419, 437, 448
SfaNI GCATC 3 cut(s) 12, 39, 262
SfcI CTRYAG 1 cut(s) 315
SinI GGWCC 2 cut(s) 325, 443
SmiMI CAYNNNNRTG 1 cut(s) 365
Sse9I AATT 2 cut(s) 155, 235
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 347
TasI AATT 2 cut(s) 155, 235
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 2 cut(s) 90, 106
TspDTI ATGAA 1 cut(s) 428
TspRI CASTG 2 cut(s) 90, 106
Tth111I GACNNNGTC 1 cut(s) 287
VpaK11BI GGWCC 2 cut(s) 325, 443
XapI RAATTY 2 cut(s) 155, 235
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XmiI GTMKAC 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.