MD16G1151500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011555
Physical Location & Seq
Reverse (-)
11951552 .. 11951989
438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1151500.v1.1.491

Sequence Viewer

Length: 438 bp
ATGCTGATGCCGGATCCACCTTTTTCGGCTTCATCAGAGACATTGTTTCAGTGTGATCTCTCACTGTCTCCGGAGGCTAGTGTTAATAAGGATGGACCGGGCAATGTCTCTCTGAGTACTGGTAAATCGTGCGAATTTGATGGATCGAACTTTGGTTCTGTGGAGGCTGACATTGTAGCGAGATTTCTCAGGCAAGCACGGACTCAGGTCTTGAATTCTGAGCTGGATACTAAATCTAAGAAGATTCTGGATGCACTGGTTGAGATTGTCATAGAGGAGCTCCACACTTCGCCTGCAGAGAGGGACCGGGTTGCTGAACTTGTGTCGGCAAAAGCTTACATAACGATGGCGTGTTTCCTGTTCTGGATCATTGCATTGGCGGTATGGGTGTTCAGCTCTGGAGGCCGAAGCACTTTCCATGGACCTCTGCCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.73

Weight (kDa)

4.83

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 70
AciI CCGC 1 cut(s) 380
AclWI GGATC 4 cut(s) 8, 21, 151, 374
AcsI RAATTY 2 cut(s) 134, 214
AfaI GTAC 1 cut(s) 118
AgsI TTSAA 1 cut(s) 214
AluBI AGCT 4 cut(s) 223, 280, 335, 396
AluI AGCT 4 cut(s) 223, 280, 335, 396
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 3 cut(s) 32, 72, 112
AlwI GGATC 4 cut(s) 8, 21, 151, 374
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 1 cut(s) 403
ApoI RAATTY 2 cut(s) 134, 214
AspS9I GGNCC 3 cut(s) 95, 304, 422
AsuC2I CCSGG 2 cut(s) 99, 308
AvaII GGWCC 3 cut(s) 95, 304, 422
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 282
BccI CCATC 3 cut(s) 86, 134, 340
BciVI GTATCC 1 cut(s) 220
BcnI CCSGG 2 cut(s) 99, 308
BcoDI GTCTC 3 cut(s) 32, 72, 112
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 294
BfuI GTATCC 1 cut(s) 220
BmcAI AGTACT 1 cut(s) 118
Bme1390I CCNGG 2 cut(s) 99, 308
Bme18I GGWCC 3 cut(s) 95, 304, 422
BmgT120I GGNCC 3 cut(s) 95, 304, 422
BmiI GGNNCC 2 cut(s) 15, 305
BmrFI CCNGG 2 cut(s) 99, 308
BmsI GCATC 1 cut(s) 241
BoxI GACNNNNGTC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 420
BpuMI CCSGG 2 cut(s) 99, 308
BsaJI CCNNGG 1 cut(s) 418
BsaWI WCCGGW 1 cut(s) 70
Bse1I ACTGG 2 cut(s) 124, 261
Bse3DI GCAATG 2 cut(s) 109, 369
BseAI TCCGGA 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 2 cut(s) 97, 256
BseMI GCAATG 2 cut(s) 109, 369
BseMII CTCAG 4 cut(s) 104, 202, 210, 218
BseNI ACTGG 2 cut(s) 124, 261
BseRI GAGGAG 1 cut(s) 290
BshFI GGCC 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 4 cut(s) 11, 71, 98, 307
BslFI GGGAC 1 cut(s) 317
BsmAI GTCTC 3 cut(s) 32, 72, 112
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 282
Bsp13I TCCGGA 1 cut(s) 70
Bsp143I GATC 4 cut(s) 13, 55, 143, 366
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 1 cut(s) 380
BspANI GGCC 1 cut(s) 405
BspCNI CTCAG 4 cut(s) 105, 201, 211, 217
BspEI TCCGGA 1 cut(s) 70
BspLI GGNNCC 2 cut(s) 15, 305
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 4 cut(s) 8, 21, 151, 374
BsrDI GCAATG 2 cut(s) 109, 369
BsrI ACTGG 2 cut(s) 124, 261
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 4 cut(s) 13, 55, 143, 366
BssT1I CCWWGG 1 cut(s) 418
Bst4CI ACNGT 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 195, 294
BstDEI CTNAG 5 cut(s) 113, 188, 204, 219, 237
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 2 cut(s) 97, 256
BstKTI GATC 4 cut(s) 16, 58, 146, 369
BstMAI GTCTC 3 cut(s) 32, 72, 112
BstMBI GATC 4 cut(s) 13, 55, 143, 366
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstPAI GACNNNNGTC 1 cut(s) 206
BstSCI CCNGG 2 cut(s) 97, 306
BstSFI CTRYAG 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuI GTATCC 1 cut(s) 220
BsuRI GGCC 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 418
BtsCI GGATG 2 cut(s) 97, 256
BtsIMutI CAGTG 3 cut(s) 56, 62, 254
Cac8I GCNNGC 2 cut(s) 195, 294
Cfr13I GGNCC 3 cut(s) 95, 304, 422
Csp6I GTAC 1 cut(s) 117
CviAII CATG 1 cut(s) 419
CviJI RGCY 8 cut(s) 29, 77, 167, 223, 280, 335, 396, 405
CviKI_1 RGCY 8 cut(s) 29, 77, 167, 223, 280, 335, 396, 405
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 5 cut(s) 113, 188, 204, 219, 237
DpnI GATC 4 cut(s) 15, 57, 145, 368
DpnII GATC 4 cut(s) 13, 55, 143, 366
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 3 cut(s) 95, 304, 422
Eco53kI GAGCTC 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 1 cut(s) 422
FaiI YATR 4 cut(s) 272, 341, 385, 420
FaqI GGGAC 1 cut(s) 317
FatI CATG 1 cut(s) 418
FokI GGATG 2 cut(s) 104, 263
FriOI GRGCYC 1 cut(s) 282
FspBI CTAG 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 420
HaeIII GGCC 1 cut(s) 405
HapII CCGG 4 cut(s) 11, 71, 98, 307
Hin1II CATG 1 cut(s) 422
HindIII AAGCTT 1 cut(s) 333
HinfI GANTC 2 cut(s) 202, 244
HpaII CCGG 4 cut(s) 11, 71, 98, 307
Hpy188I TCNGA 3 cut(s) 37, 114, 220
Hpy188III TCNNGA 5 cut(s) 71, 211, 248, 364, 399
HpyCH4III ACNGT 1 cut(s) 66
HpyCH4V TGCA 3 cut(s) 254, 296, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpyF3I CTNAG 5 cut(s) 113, 188, 204, 219, 237
Hsp92II CATG 1 cut(s) 422
Kpn2I TCCGGA 1 cut(s) 70
Kzo9I GATC 4 cut(s) 13, 55, 143, 366
LmnI GCTCC 2 cut(s) 277, 285
LweI GCATC 1 cut(s) 241
MaeI CTAG 1 cut(s) 78
MalI GATC 4 cut(s) 15, 57, 145, 368
MboI GATC 4 cut(s) 13, 55, 143, 366
MboII GAAGA 1 cut(s) 253
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 2 cut(s) 134, 214
MlyI GAGTC 1 cut(s) 196
MnlI CCTC 6 cut(s) 67, 157, 268, 294, 395, 435
MroI TCCGGA 1 cut(s) 70
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 344
MspI CCGG 4 cut(s) 11, 71, 98, 307
MspR9I CCNGG 2 cut(s) 99, 308
MwoI GCNNNNNNNGC 1 cut(s) 402
NciI CCSGG 2 cut(s) 99, 308
NcoI CCATGG 1 cut(s) 418
NdeII GATC 4 cut(s) 13, 55, 143, 366
NlaIII CATG 1 cut(s) 422
NlaIV GGNNCC 2 cut(s) 15, 305
PfeI GAWTC 1 cut(s) 244
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PshAI GACNNNNGTC 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 282
PspN4I GGNNCC 2 cut(s) 15, 305
PspPI GGNCC 3 cut(s) 95, 304, 422
PstI CTGCAG 1 cut(s) 298
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
RseI CAYNNNNRTG 1 cut(s) 344
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 1 cut(s) 84
Sau3AI GATC 4 cut(s) 13, 55, 143, 366
Sau96I GGNCC 3 cut(s) 95, 304, 422
ScaI AGTACT 1 cut(s) 118
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 2 cut(s) 99, 308
SduI GDGCHC 1 cut(s) 282
SetI ASST 7 cut(s) 22, 210, 225, 282, 337, 398, 427
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 3 cut(s) 95, 304, 422
SmiMI CAYNNNNRTG 1 cut(s) 344
Sse9I AATT 2 cut(s) 134, 214
SsiI CCGC 1 cut(s) 380
SspMI CTAG 1 cut(s) 78
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 2 cut(s) 97, 306
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 146
TasI AATT 2 cut(s) 134, 214
TatI WGTACW 1 cut(s) 116
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 3 cut(s) 56, 69, 261
TspDTI ATGAA 1 cut(s) 21
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 3 cut(s) 56, 69, 261
VpaK11BI GGWCC 3 cut(s) 95, 304, 422
XapI RAATTY 2 cut(s) 134, 214
XspI CTAG 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.