pycom13g12980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011555
Physical Location & Seq
Reverse (-)
9193037 .. 9194200
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g12980.1

Sequence Viewer

Length: 816 bp
ATGGACCTTTTGTTGTGTCTCTTCTTTCTTCTGCATTTTAGGCCCATATATAACATCGTCGCCCAAACGCGCTCTTTAAAAAGAAAGGAAAATCAAAACTCGTTTTTCTCCCGCCAAGCAAACGAACCAGAGAAAATGAGAGAGAACCGAACACCGCTCAAGGACCAATCCCGACCTGTGAAGCTCGCGACTCCCGTTCCCAAGAAGAAGATTCCGAAGAAGAGCTTGAACACCGTCTTTTCTGCAGTTTCGGAAGACGAACCGGTGGATAATTTCAAGATTTCAGTGGATTCTACTCCGATTTCTGAGAGTTCCAAGATTTCAGTGGATTACACTCCGATTTCTGTGATTTCTGCTGTGGATTTCACCGAGAGTATGATGCTGGTGCCGGATCCAGCTCTGTCGATGCCATCAGAGACATTGCTTCCATCCAATGTCTCACTGTCTCCGGAGGCCAGTGTCAATAAGGATGGACTGGGCAATGTCTCTCTAAGTAGTGGTAAATCAAGCGAATTTGATGGCTCGAACTTTGGTTCTATGGAGGCTGACATTGTGGAGAGATTTCTCAGGCAAGCGCGGACTCAGGTCTTGAATTCTGAGGTGGATACTAAATCTAAGAAGATTCTGGATGCGCTGGTTGACATTGTCTTAGAGGAGCTCCACACTTCACCTGCAGAGAGGGACTGGGTAACTGAACTTCTGTCGACAAAAATTTACATAACAATGGTGTGTTTTGTGCTCTGGATCATTGGATTGGCTGTCTGGATGTTCGGCTCTGGACGCCGAAACACTTTTCGTGGACCTCTGCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

30.29

Weight (kDa)

6.21

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 679
Acc36I ACCTGC 1 cut(s) 679
AccB1I GGYRCC 1 cut(s) 385
AccBSI CCGCTC 1 cut(s) 157
AccI GTMKAC 1 cut(s) 704
AccII CGCG 3 cut(s) 70, 188, 577
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 3 cut(s) 112, 155, 577
AclWI GGATC 3 cut(s) 386, 399, 752
AcsI RAATTY 3 cut(s) 512, 592, 711
AcyI GRCGYC 1 cut(s) 781
AgeI ACCGGT 1 cut(s) 262
AgsI TTSAA 3 cut(s) 229, 277, 592
AluBI AGCT 4 cut(s) 184, 225, 398, 658
AluI AGCT 4 cut(s) 184, 225, 398, 658
Alw21I GWGCWC 2 cut(s) 660, 741
Alw26I GTCTC 5 cut(s) 23, 410, 442, 450, 490
AlwI GGATC 3 cut(s) 386, 399, 752
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 2 cut(s) 41, 453
ApoI RAATTY 3 cut(s) 512, 592, 711
AsiGI ACCGGT 1 cut(s) 262
AspLEI GCGC 3 cut(s) 72, 577, 634
AspS9I GGNCC 4 cut(s) 4, 42, 163, 800
AsuHPI GGTGA 2 cut(s) 358, 660
AvaII GGWCC 3 cut(s) 4, 163, 800
BamHI GGATCC 1 cut(s) 391
BanI GGYRCC 1 cut(s) 385
BanII GRGCYC 1 cut(s) 660
BarI GAAGNNNNNNTAC 2 cut(s) 681, 713
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 2 cut(s) 660, 741
BccI CCATC 4 cut(s) 418, 436, 464, 512
BciVI GTATCC 1 cut(s) 598
BcoDI GTCTC 5 cut(s) 23, 410, 442, 450, 490
BfmI CTRYAG 2 cut(s) 243, 672
BfuAI ACCTGC 1 cut(s) 679
BfuI GTATCC 1 cut(s) 598
Bme18I GGWCC 3 cut(s) 4, 163, 800
BmgT120I GGNCC 4 cut(s) 4, 42, 163, 800
BmiI GGNNCC 2 cut(s) 387, 393
BmrI ACTGGG 2 cut(s) 485, 694
BmsI GCATC 3 cut(s) 369, 396, 619
BmuI ACTGGG 2 cut(s) 485, 694
BoxI GACNNNNGTC 1 cut(s) 584
BpiI GAAGAC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 143
BsaHI GRCGYC 1 cut(s) 781
BsaWI WCCGGW 2 cut(s) 262, 448
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 3 cut(s) 456, 480, 689
Bse3DI GCAATG 2 cut(s) 419, 487
BseAI TCCGGA 1 cut(s) 448
BseGI GGATG 4 cut(s) 428, 475, 634, 771
BseMI GCAATG 2 cut(s) 419, 487
BseMII CTCAG 4 cut(s) 297, 580, 588, 596
BseNI ACTGG 3 cut(s) 456, 480, 689
BseRI GAGGAG 1 cut(s) 668
Bsh1236I CGCG 3 cut(s) 70, 188, 577
BshFI GGCC 2 cut(s) 43, 455
BshNI GGYRCC 1 cut(s) 385
BshTI ACCGGT 1 cut(s) 262
BsiHKAI GWGCWC 2 cut(s) 660, 741
BsiSI CCGG 3 cut(s) 263, 389, 449
BslFI GGGAC 1 cut(s) 695
BsmAI GTCTC 5 cut(s) 23, 410, 442, 450, 490
BsmFI GGGAC 1 cut(s) 695
BsnI GGCC 2 cut(s) 43, 455
Bsp1286I GDGCHC 2 cut(s) 660, 741
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 2 cut(s) 391, 744
Bsp68I TCGCGA 1 cut(s) 188
BspACI CCGC 3 cut(s) 112, 155, 577
BspANI GGCC 2 cut(s) 43, 455
BspCNI CTCAG 4 cut(s) 298, 579, 589, 595
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 3 cut(s) 70, 188, 577
BspLI GGNNCC 2 cut(s) 387, 393
BspMAI CTGCAG 2 cut(s) 247, 676
BspMI ACCTGC 1 cut(s) 679
BspPI GGATC 3 cut(s) 386, 399, 752
BspQI GCTCTTC 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 385
BsrBI CCGCTC 1 cut(s) 157
BsrDI GCAATG 2 cut(s) 419, 487
BsrFI RCCGGY 1 cut(s) 262
BsrI ACTGG 3 cut(s) 456, 480, 689
BssAI RCCGGY 1 cut(s) 262
BssMI GATC 2 cut(s) 391, 744
BssNI GRCGYC 1 cut(s) 781
Bst4CI ACNGT 2 cut(s) 235, 444
Bst6I CTCTTC 2 cut(s) 26, 215
BstACI GRCGYC 1 cut(s) 781
BstC8I GCNNGC 2 cut(s) 186, 573
BstDEI CTNAG 7 cut(s) 306, 491, 566, 582, 597, 615, 649
BstF5I GGATG 4 cut(s) 428, 475, 634, 771
BstFNI CGCG 3 cut(s) 70, 188, 577
BstHHI GCGC 3 cut(s) 72, 577, 634
BstKTI GATC 2 cut(s) 394, 747
BstMAI GTCTC 5 cut(s) 23, 410, 442, 450, 490
BstMBI GATC 2 cut(s) 391, 744
BstMWI GCNNNNNNNGC 2 cut(s) 40, 780
BstPAI GACNNNNGTC 1 cut(s) 584
BstSFI CTRYAG 2 cut(s) 243, 672
BstUI CGCG 3 cut(s) 70, 188, 577
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuI GTATCC 1 cut(s) 598
BsuRI GGCC 2 cut(s) 43, 455
BtsCI GGATG 4 cut(s) 428, 475, 634, 771
BtsIMutI CAGTG 4 cut(s) 291, 330, 440, 463
BtuMI TCGCGA 1 cut(s) 188
BveI ACCTGC 1 cut(s) 679
Cac8I GCNNGC 2 cut(s) 186, 573
CfoI GCGC 3 cut(s) 72, 577, 634
Cfr10I RCCGGY 1 cut(s) 262
Cfr13I GGNCC 4 cut(s) 4, 42, 163, 800
CseI GACGC 1 cut(s) 789
CspAI ACCGGT 1 cut(s) 262
DdeI CTNAG 7 cut(s) 306, 491, 566, 582, 597, 615, 649
DpnI GATC 2 cut(s) 393, 746
DpnII GATC 2 cut(s) 391, 744
DraI TTTAAA 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 26, 215
EarI CTCTTC 2 cut(s) 26, 215
Ecl136II GAGCTC 1 cut(s) 658
Eco24I GRGCYC 1 cut(s) 660
Eco47I GGWCC 3 cut(s) 4, 163, 800
Eco53kI GAGCTC 1 cut(s) 658
EcoICRI GAGCTC 1 cut(s) 658
EcoRI GAATTC 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 660
FaiI YATR 6 cut(s) 47, 49, 51, 377, 539, 719
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FaqI GGGAC 1 cut(s) 695
FauI CCCGC 1 cut(s) 119
FblI GTMKAC 1 cut(s) 704
FokI GGATG 4 cut(s) 415, 482, 641, 778
FriOI GRGCYC 1 cut(s) 660
GlaI GCGC 3 cut(s) 71, 576, 633
HaeIII GGCC 2 cut(s) 43, 455
HapII CCGG 3 cut(s) 263, 389, 449
HgaI GACGC 1 cut(s) 789
HhaI GCGC 3 cut(s) 72, 577, 634
Hin1I GRCGYC 1 cut(s) 781
Hin6I GCGC 3 cut(s) 70, 575, 632
HinP1I GCGC 3 cut(s) 70, 575, 632
HincII GTYRAC 2 cut(s) 640, 705
HindII GTYRAC 2 cut(s) 640, 705
HinfI GANTC 5 cut(s) 190, 211, 290, 580, 622
HpaII CCGG 3 cut(s) 263, 389, 449
HphI GGTGA 2 cut(s) 358, 660
Hpy166II GTNNAC 3 cut(s) 640, 705, 800
Hpy188I TCNGA 7 cut(s) 216, 253, 300, 307, 339, 415, 598
Hpy188III TCNNGA 9 cut(s) 171, 187, 277, 449, 589, 626, 742, 763, 777
Hpy8I GTNNAC 3 cut(s) 640, 705, 800
Hpy99I CGWCG 1 cut(s) 62
HpyCH4III ACNGT 2 cut(s) 235, 444
HpyCH4V TGCA 3 cut(s) 34, 245, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 780
HpyF3I CTNAG 7 cut(s) 306, 491, 566, 582, 597, 615, 649
Hsp92I GRCGYC 1 cut(s) 781
HspAI GCGC 3 cut(s) 70, 575, 632
Kpn2I TCCGGA 1 cut(s) 448
Kzo9I GATC 2 cut(s) 391, 744
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 2 cut(s) 655, 663
LweI GCATC 3 cut(s) 369, 396, 619
MaeIII GTNAC 1 cut(s) 688
MalI GATC 2 cut(s) 393, 746
MbiI CCGCTC 1 cut(s) 157
MboI GATC 2 cut(s) 391, 744
MboII GAAGA 8 cut(s) 13, 20, 217, 220, 229, 232, 266, 631
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 2 cut(s) 660, 741
MluCI AATT 4 cut(s) 271, 512, 592, 711
MlyI GAGTC 2 cut(s) 184, 574
MnlI CCTC 6 cut(s) 445, 535, 592, 646, 672, 813
MroI TCCGGA 1 cut(s) 448
MseI TTAA 1 cut(s) 77
MslI CAYNNNNRTG 1 cut(s) 722
MspI CCGG 3 cut(s) 263, 389, 449
MvnI CGCG 3 cut(s) 70, 188, 577
MwoI GCNNNNNNNGC 2 cut(s) 40, 780
NdeII GATC 2 cut(s) 391, 744
NlaIV GGNNCC 2 cut(s) 387, 393
NruI TCGCGA 1 cut(s) 188
PaqCI CACCTGC 1 cut(s) 679
PciSI GCTCTTC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PfeI GAWTC 3 cut(s) 211, 290, 622
PflFI GACNNNGTC 1 cut(s) 644
PinAI ACCGGT 1 cut(s) 262
PleI GAGTC 2 cut(s) 184, 574
PpsI GAGTC 2 cut(s) 184, 574
PshAI GACNNNNGTC 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 660
PspN4I GGNNCC 2 cut(s) 387, 393
PspPI GGNCC 4 cut(s) 4, 42, 163, 800
PstI CTGCAG 2 cut(s) 247, 676
PsuI RGATCY 1 cut(s) 391
PsyI GACNNNGTC 1 cut(s) 644
RruI TCGCGA 1 cut(s) 188
RseI CAYNNNNRTG 1 cut(s) 722
SacI GAGCTC 1 cut(s) 660
SalI GTCGAC 1 cut(s) 703
SapI GCTCTTC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 77
Sau3AI GATC 2 cut(s) 391, 744
Sau96I GGNCC 4 cut(s) 4, 42, 163, 800
SchI GAGTC 2 cut(s) 184, 574
SduI GDGCHC 2 cut(s) 660, 741
SfaNI GCATC 3 cut(s) 369, 396, 619
SfcI CTRYAG 2 cut(s) 243, 672
SinI GGWCC 3 cut(s) 4, 163, 800
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
Sse9I AATT 4 cut(s) 271, 512, 592, 711
SsiI CCGC 3 cut(s) 112, 155, 577
SstI GAGCTC 1 cut(s) 660
TaaI ACNGT 2 cut(s) 235, 444
TaqI TCGA 3 cut(s) 404, 524, 704
TasI AATT 4 cut(s) 271, 512, 592, 711
TfiI GAWTC 3 cut(s) 211, 290, 622
Tru1I TTAA 1 cut(s) 77
Tru9I TTAA 1 cut(s) 77
TscAI CASTG 4 cut(s) 291, 330, 447, 463
TspRI CASTG 4 cut(s) 291, 330, 447, 463
Tth111I GACNNNGTC 1 cut(s) 644
VpaK11BI GGWCC 3 cut(s) 4, 163, 800
XapI RAATTY 3 cut(s) 512, 592, 711
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.