FvH4_4g20660

Ribosomal protein S6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013271
Physical Location & Seq
Reverse (-)
23793235 .. 23795772
2538 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20660.t1

Sequence Viewer

Length: 885 bp
ATGGGGGCTTTCTTACACTCCTCTGTAGCTCTCTTCACTCCACCGGCTCTGAAACTGGTCACCAGAAATGGGTCTTCTCGGAATCACAGTTCTTGCTCCGAATTCTCAACTGGGTGCCCTTCTTTTTCTTGTTCTTGCAGACCATTTAGAGCTCAGAAGCTACCAATTGGGCCCAGAAGGTCAGTGATTGTTGGGGCTAAGAAGAAGAACAACAAGGAGAGGAAGAAGGAAGATAAGCATAGCTTTGTGTCTAAGCCTGATGAGGCTACTGGGCATTTTCCTGAAGCTGTTCTGCTTAGACAGAAGAAAGTTCAGGAAGATGGTGCAGTTCTCCCTGAGTTTGCCGATGAAGACGAAGAAAAGATCTATGAATTTCTGAAGCTTCAGCTGCAAGACGATCTGGATGAGGAACGACTGCGCCACTATGAGGTGGTGTATCTAATTCATGAGAAGCATGCAGAAGAGGTGGGGAGTGTAAAGGAGAAAATTGAAGCTTTTCTAGGGGAGAAGAGAGCCAGGATATGGAGACTGAGTGATTGGGGAATGCGAAGACTAGCATACAAAATACAAAAAGCCAAGAATGCCTACTACGTTCTGATGAACTTTGAGATTGATGCCAAATGGATAAATGATTTCAAGAGTCTGTTGGACAAGGATGAAAGAGTCATTCGGCATCTGGTGATGAAGAGGGATGAGGCTATCACAGAGGATTGTCCTCCTCCTTCTGAGTTTCAGTCCATGAATGCCGGTACAGATTCTGAAGAGGAAGATGAGGATGATATTGAATATGATGATGAAGATTACGATGATGAAGATGGGATTATCATTGTTGATGCTGATAATGATGATGTAGAAAATAGTAAACTAGTAGAGCAACTAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S6

Protein Analysis

295

Amino Acids

33.92

Weight (kDa)

4.94

Isoelectric Point (pI)

52.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S6 PF01250 141 - 229 6.9e-24 Ribosomal protein S6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17170
fragaria_vesca FvH4_4g20660
malus_domestica MD13G1149000.v1.1 MD16G1149200.v1.1
prunus_persica Prupe.1G113000_v2.0.a1
pyrus_communis pycom13g12820 pycom16g12750
rosa_chinensis RchiOBHm_Chr4g0426061
rosa_laevigata RLG00000007320
rosa_multiflora Rmu_ssc0000281.1_g000002 Rmu_ssc0000281.1_g000007
rosa_roxburghii Rroxscaffold_5G00368060
rosa_rugosa Rorug04G0208200
rosa_samantha Rh4AG265200 Rh4BG271300 Rh4CG284300 Rh4DG267600
rosa_wichuraiana Rw4G022930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 114
AccB7I CCANNNNNTGG 1 cut(s) 522
AcsI RAATTY 2 cut(s) 101, 371
AcuI CTGAAG 4 cut(s) 303, 368, 398, 780
AfaI GTAC 1 cut(s) 751
AfiI CCNNNNNNNGG 3 cut(s) 69, 427, 522
AgsI TTSAA 3 cut(s) 491, 637, 785
AhlI ACTAGT 2 cut(s) 865, 877
AjnI CCWGG 1 cut(s) 515
AloI GAACNNNNNNTCC 2 cut(s) 73, 105
AluBI AGCT 8 cut(s) 29, 152, 160, 243, 287, 382, 388, 494
AluI AGCT 8 cut(s) 29, 152, 160, 243, 287, 382, 388, 494
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 520
AlwNI CAGNNNCTG 1 cut(s) 758
AoxI GGCC 1 cut(s) 170
ApaI GGGCCC 1 cut(s) 174
ApeKI GCWGC 1 cut(s) 388
ApoI RAATTY 2 cut(s) 101, 371
Asp700I GAANNNNTTC 2 cut(s) 288, 495
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 2 cut(s) 170, 171
AsuHPI GGTGA 2 cut(s) 52, 691
BaeGI GKGCMC 2 cut(s) 119, 174
BanI GGYRCC 1 cut(s) 114
BanII GRGCYC 2 cut(s) 154, 174
BbsI GAAGAC 3 cut(s) 66, 357, 556
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 1 cut(s) 375
BccI CCATC 2 cut(s) 314, 809
BciT130I CCWGG 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 520
BcuI ACTAGT 2 cut(s) 865, 877
BfaI CTAG 4 cut(s) 500, 554, 866, 878
BfmI CTRYAG 1 cut(s) 24
BglII AGATCT 1 cut(s) 363
BisI GCNGC 1 cut(s) 389
BlsI GCNGC 1 cut(s) 390
Bme1390I CCNGG 1 cut(s) 517
BmgT120I GGNCC 2 cut(s) 170, 171
BmiI GGNNCC 2 cut(s) 116, 172
BmrFI CCNGG 1 cut(s) 517
BmrI ACTGGG 2 cut(s) 120, 279
BmsI GCATC 3 cut(s) 604, 682, 823
BmuI ACTGGG 2 cut(s) 120, 279
BpiI GAAGAC 3 cut(s) 66, 357, 556
BsaXI ACNNNNNCTCC 2 cut(s) 517, 547
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 427, 522
Bse118I RCCGGY 2 cut(s) 43, 746
Bse1I ACTGG 3 cut(s) 60, 115, 274
BseBI CCWGG 1 cut(s) 517
BseGI GGATG 4 cut(s) 409, 661, 697, 781
BseLI CCNNNNNNNGG 3 cut(s) 69, 427, 522
BseMII CTCAG 4 cut(s) 167, 327, 521, 717
BseNI ACTGG 3 cut(s) 60, 115, 274
BseRI GAGGAG 2 cut(s) 10, 708
BseSI GKGCMC 2 cut(s) 119, 174
BseXI GCAGC 1 cut(s) 375
BsgI GTGCAG 1 cut(s) 345
BshFI GGCC 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 114
BsiHKAI GWGCWC 1 cut(s) 154
BsiSI CCGG 2 cut(s) 44, 747
BslI CCNNNNNNNGG 3 cut(s) 69, 427, 522
BsmAI GTCTC 1 cut(s) 520
BsmI GAATGC 3 cut(s) 549, 586, 748
BsnI GGCC 1 cut(s) 172
Bsp120I GGGCCC 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 119, 154, 174
Bsp143I GATC 2 cut(s) 363, 397
BspANI GGCC 1 cut(s) 172
BspCNI CTCAG 4 cut(s) 166, 328, 522, 718
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 2 cut(s) 116, 172
BspT107I GGYRCC 1 cut(s) 114
BsrFI RCCGGY 2 cut(s) 43, 746
BsrI ACTGG 3 cut(s) 60, 115, 274
BssAI RCCGGY 2 cut(s) 43, 746
BssMI GATC 2 cut(s) 363, 397
Bst2UI CCWGG 1 cut(s) 517
Bst4CI ACNGT 1 cut(s) 89
Bst6I CTCTTC 5 cut(s) 38, 456, 503, 680, 756
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 7 cut(s) 153, 198, 252, 296, 336, 530, 726
BstEII GGTNACC 1 cut(s) 58
BstF5I GGATG 4 cut(s) 409, 661, 697, 781
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 2 cut(s) 366, 400
BstMAI GTCTC 1 cut(s) 520
BstMBI GATC 2 cut(s) 363, 397
BstMWI GCNNNNNNNGC 2 cut(s) 388, 581
BstNI CCWGG 1 cut(s) 517
BstNSI RCATGY 1 cut(s) 458
BstPI GGTNACC 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 515
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 2 cut(s) 119, 174
BstV1I GCAGC 1 cut(s) 375
BstV2I GAAGAC 3 cut(s) 66, 357, 556
BstX2I RGATCY 1 cut(s) 363
BstYI RGATCY 1 cut(s) 363
BsuRI GGCC 1 cut(s) 172
BtsCI GGATG 4 cut(s) 409, 661, 697, 781
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 456
CaiI CAGNNNCTG 1 cut(s) 758
CciI TCATGA 1 cut(s) 445
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 2 cut(s) 43, 746
Cfr13I GGNCC 2 cut(s) 170, 171
Csp6I GTAC 1 cut(s) 750
CviAII CATG 3 cut(s) 446, 455, 739
CviQI GTAC 1 cut(s) 750
DdeI CTNAG 7 cut(s) 153, 198, 252, 296, 336, 530, 726
DpnI GATC 2 cut(s) 365, 399
DpnII GATC 2 cut(s) 363, 397
Eam1104I CTCTTC 5 cut(s) 38, 456, 503, 680, 756
EarI CTCTTC 5 cut(s) 38, 456, 503, 680, 756
Ecl136II GAGCTC 1 cut(s) 152
Eco24I GRGCYC 2 cut(s) 154, 174
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 4 cut(s) 303, 368, 398, 780
Eco91I GGTNACC 1 cut(s) 58
EcoICRI GAGCTC 1 cut(s) 152
EcoO65I GGTNACC 1 cut(s) 58
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 515
EcoT38I GRGCYC 2 cut(s) 154, 174
FaeI CATG 3 cut(s) 449, 458, 742
FaiI YATR 9 cut(s) 240, 369, 426, 447, 456, 523, 559, 740, 789
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FatI CATG 3 cut(s) 445, 454, 738
Fnu4HI GCNGC 1 cut(s) 389
FokI GGATG 4 cut(s) 416, 668, 704, 788
FriOI GRGCYC 2 cut(s) 154, 174
Fsp4HI GCNGC 1 cut(s) 389
FspBI CTAG 4 cut(s) 500, 554, 866, 878
GlaI GCGC 1 cut(s) 419
GluI GCNGC 1 cut(s) 389
HaeIII GGCC 1 cut(s) 172
HapII CCGG 2 cut(s) 44, 747
HhaI GCGC 1 cut(s) 420
Hin1II CATG 3 cut(s) 449, 458, 742
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HindIII AAGCTT 2 cut(s) 380, 492
HinfI GANTC 4 cut(s) 82, 640, 663, 755
HpaII CCGG 2 cut(s) 44, 747
HphI GGTGA 2 cut(s) 52, 691
Hpy166II GTNNAC 1 cut(s) 863
Hpy188I TCNGA 8 cut(s) 51, 81, 100, 156, 378, 597, 727, 760
Hpy188III TCNNGA 5 cut(s) 281, 314, 401, 446, 637
Hpy8I GTNNAC 1 cut(s) 863
HpyAV CCTTC 4 cut(s) 129, 171, 220, 732
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 4 cut(s) 138, 326, 391, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 388, 581
HpyF3I CTNAG 7 cut(s) 153, 198, 252, 296, 336, 530, 726
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 3 cut(s) 449, 458, 742
HspAI GCGC 1 cut(s) 418
Kzo9I GATC 2 cut(s) 363, 397
LmnI GCTCC 1 cut(s) 101
Lsp1109I GCAGC 1 cut(s) 375
LweI GCATC 3 cut(s) 604, 682, 823
MaeI CTAG 4 cut(s) 500, 554, 866, 878
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 1 cut(s) 58
MalI GATC 2 cut(s) 365, 399
MboI GATC 2 cut(s) 363, 397
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 3 cut(s) 119, 154, 174
MluCI AATT 5 cut(s) 101, 165, 371, 441, 486
MlyI GAGTC 2 cut(s) 649, 672
MmeI TCCRAC 1 cut(s) 627
MroXI GAANNNNTTC 2 cut(s) 288, 495
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 2 cut(s) 44, 747
MspR9I CCNGG 1 cut(s) 517
MunI CAATTG 1 cut(s) 165
Mva1269I GAATGC 3 cut(s) 549, 586, 748
MvaI CCWGG 1 cut(s) 517
MwoI GCNNNNNNNGC 2 cut(s) 388, 581
NdeII GATC 2 cut(s) 363, 397
NlaIII CATG 3 cut(s) 449, 458, 742
NlaIV GGNNCC 2 cut(s) 116, 172
NmuCI GTSAC 1 cut(s) 58
NspI RCATGY 1 cut(s) 458
PaeI GCATGC 1 cut(s) 458
PagI TCATGA 1 cut(s) 445
PctI GAATGC 3 cut(s) 549, 586, 748
PdmI GAANNNNTTC 2 cut(s) 288, 495
PfeI GAWTC 2 cut(s) 82, 755
PflMI CCANNNNNTGG 1 cut(s) 522
PkrI GCNGC 1 cut(s) 390
PleI GAGTC 2 cut(s) 648, 671
PpsI GAGTC 2 cut(s) 648, 671
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 1 cut(s) 515
PspEI GGTNACC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 515
PspN4I GGNNCC 2 cut(s) 116, 172
PspOMI GGGCCC 1 cut(s) 170
PspPI GGNCC 2 cut(s) 170, 171
PstNI CAGNNNCTG 1 cut(s) 758
PsuI RGATCY 1 cut(s) 363
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 1 cut(s) 751
RsaNI GTAC 1 cut(s) 750
SacI GAGCTC 1 cut(s) 154
SatI GCNGC 1 cut(s) 389
Sau3AI GATC 2 cut(s) 363, 397
Sau96I GGNCC 2 cut(s) 170, 171
SchI GAGTC 2 cut(s) 649, 672
ScrFI CCNGG 1 cut(s) 517
SduI GDGCHC 3 cut(s) 119, 154, 174
SfaNI GCATC 3 cut(s) 604, 682, 823
SfcI CTRYAG 1 cut(s) 24
SpeI ACTAGT 2 cut(s) 865, 877
SphI GCATGC 1 cut(s) 458
Sse9I AATT 5 cut(s) 101, 165, 371, 441, 486
SspMI CTAG 4 cut(s) 500, 554, 866, 878
SstI GAGCTC 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 515
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 1 cut(s) 594
TasI AATT 5 cut(s) 101, 165, 371, 441, 486
TfiI GAWTC 2 cut(s) 82, 755
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 1 cut(s) 388
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 9 cut(s) 363, 384, 434, 614, 672, 698, 755, 810, 825
TspRI CASTG 1 cut(s) 189
Van91I CCANNNNNTGG 1 cut(s) 522
XapI RAATTY 2 cut(s) 101, 371
XceI RCATGY 1 cut(s) 458
XmnI GAANNNNTTC 2 cut(s) 288, 495
XspI CTAG 4 cut(s) 500, 554, 866, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.