Rroxscaffold_5G00368060

Ribosomal protein S6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013271
Physical Location & Seq
Reverse (-)
49827948 .. 49830481
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00368060.1

Sequence Viewer

Length: 852 bp
ATGGCCTCGGTAGCTTTCTTCTCTCCACCGGCTCTGAAACTGGTCACCGGAAATGGGTCTTCTCGGAACCAGAGTTCTTGCTCGGAATTCTCAAATGGGTACCCTTCGTTTTCTTCTTCTTGCAGACCATTTAGAGCTCAGAAGCTCTCAGTTGGGCCCAGAAGGTCAGTGATTGTTGGGGCGAAGAAGAAGAACAACAAGGAGAGGAAGAAGGAAGATAAGCATAGCTTTGTGTCTAAGCCTGATGAGGCTACTGGGCCTTTTCCTGAAGCTGTGCTGCTTAAACAGTCGAAAGTTCAGGAAGATGGGGCACTTCTCCCTGAGTTTGCGGACGCAGATGAAGAGAAGGTCTATGAATTTCTGAAGCTTCAGCTGCAAGATGATCTGGATGAGGAAAGACTGCGCCACTATGAGGTGGTTTATCTGATTCATGAGAAACATGCTGAGGAGGTGGGGAGTGTGAAGGAGAAAATTGAAGCTTTTCTAGGGGAGAAGAGAGCCAGGATATGGAGACTGAGTGATTGGGGAATGCGAAGACTAGCATACAAAATACAGAAAGCCAAGAACGCCTACTACGTTCTGATGAACTTTGAGATAGACGCCAAATGGATAAATGATTTCAAGAGCCTGTTGGACAAGGATGAAAGAGTCATTCGGCATCTGGTGATGAAGAGGGATGAGGCTATCACAGAGGACTGCCCTGCTCCTCCTGAGTTTCAGTCCATGAATGCAGGTACGGATTCTGAAGAGGATGAGGATGATATGGAATATGATGATGAAGATGGGATTATCATTGTTGACGCTGACAATGATGATGTAGAAGATAGTAAACTACTAGAACAACTAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S6

Protein Analysis

283

Amino Acids

32.41

Weight (kDa)

4.91

Isoelectric Point (pI)

52.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S6 PF01250 136 - 224 6.4e-24 Ribosomal protein S6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17170
fragaria_vesca FvH4_4g20660
malus_domestica MD13G1149000.v1.1 MD16G1149200.v1.1
prunus_persica Prupe.1G113000_v2.0.a1
pyrus_communis pycom13g12820 pycom16g12750
rosa_chinensis RchiOBHm_Chr4g0426061
rosa_laevigata RLG00000007320
rosa_multiflora Rmu_ssc0000281.1_g000002 Rmu_ssc0000281.1_g000007
rosa_roxburghii Rroxscaffold_5G00368060
rosa_rugosa Rorug04G0208200
rosa_samantha Rh4AG265200 Rh4BG271300 Rh4CG284300 Rh4DG267600
rosa_wichuraiana Rw4G022930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 722
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 507
AciI CCGC 1 cut(s) 329
AcsI RAATTY 2 cut(s) 86, 356
AcuI CTGAAG 4 cut(s) 288, 353, 383, 765
AcyI GRCGYC 1 cut(s) 600
AfaI GTAC 2 cut(s) 101, 736
AfiI CCNNNNNNNGG 3 cut(s) 54, 412, 507
AgsI TTSAA 2 cut(s) 476, 622
AhlI ACTAGT 1 cut(s) 844
AjnI CCWGG 1 cut(s) 500
AloI GAACNNNNNNTCC 2 cut(s) 58, 90
AluBI AGCT 8 cut(s) 14, 137, 145, 228, 272, 367, 373, 479
AluI AGCT 8 cut(s) 14, 137, 145, 228, 272, 367, 373, 479
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 1 cut(s) 505
AoxI GGCC 3 cut(s) 3, 155, 257
ApaI GGGCCC 1 cut(s) 159
ApeKI GCWGC 2 cut(s) 277, 373
ApoI RAATTY 2 cut(s) 86, 356
Asp700I GAANNNNTTC 1 cut(s) 480
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 155, 156, 257
AsuHPI GGTGA 2 cut(s) 37, 676
BaeGI GKGCMC 2 cut(s) 159, 313
BanI GGYRCC 1 cut(s) 99
BanII GRGCYC 2 cut(s) 139, 159
BbsI GAAGAC 2 cut(s) 51, 541
Bbv12I GWGCWC 1 cut(s) 139
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 2 cut(s) 264, 360
BccI CCATC 2 cut(s) 299, 776
BciT130I CCWGG 1 cut(s) 502
BcoDI GTCTC 1 cut(s) 505
BcuI ACTAGT 1 cut(s) 844
BfaI CTAG 4 cut(s) 485, 539, 836, 845
BfuAI ACCTGC 1 cut(s) 722
BisI GCNGC 2 cut(s) 278, 374
BlsI GCNGC 2 cut(s) 279, 375
Bme1390I CCNGG 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 155, 156, 257
BmiI GGNNCC 3 cut(s) 68, 101, 157
BmrFI CCNGG 1 cut(s) 502
BmrI ACTGGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 1 cut(s) 264
BpiI GAAGAC 2 cut(s) 51, 541
Bpu10I CCTNAGC 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 600
BsaJI CCNNGG 1 cut(s) 6
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 502, 532
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 412, 507
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 2 cut(s) 45, 259
BseBI CCWGG 1 cut(s) 502
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 5 cut(s) 394, 646, 682, 757, 763
BseLI CCNNNNNNNGG 3 cut(s) 54, 412, 507
BseMII CTCAG 6 cut(s) 152, 162, 312, 435, 506, 702
BseNI ACTGG 2 cut(s) 45, 259
BseRI GAGGAG 2 cut(s) 461, 696
BseSI GKGCMC 2 cut(s) 159, 313
BseXI GCAGC 2 cut(s) 264, 360
BshFI GGCC 3 cut(s) 5, 157, 259
BshNI GGYRCC 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 2 cut(s) 29, 48
BslI CCNNNNNNNGG 3 cut(s) 54, 412, 507
BsmAI GTCTC 1 cut(s) 505
BsmI GAATGC 2 cut(s) 534, 733
BsnI GGCC 3 cut(s) 5, 157, 259
Bsp120I GGGCCC 1 cut(s) 155
Bsp1286I GDGCHC 3 cut(s) 139, 159, 313
Bsp143I GATC 1 cut(s) 382
BspACI CCGC 1 cut(s) 329
BspANI GGCC 3 cut(s) 5, 157, 259
BspCNI CTCAG 6 cut(s) 151, 161, 313, 436, 507, 703
BspHI TCATGA 1 cut(s) 430
BspLI GGNNCC 3 cut(s) 68, 101, 157
BspMI ACCTGC 1 cut(s) 722
BspT107I GGYRCC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 28
BsrI ACTGG 2 cut(s) 45, 259
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 1 cut(s) 382
BssNI GRCGYC 1 cut(s) 600
Bst2UI CCWGG 1 cut(s) 502
Bst4CI ACNGT 1 cut(s) 288
Bst6I CTCTTC 4 cut(s) 336, 488, 665, 741
BstACI GRCGYC 1 cut(s) 600
BstDEI CTNAG 7 cut(s) 138, 148, 237, 321, 444, 515, 711
BstEII GGTNACC 1 cut(s) 43
BstF5I GGATG 5 cut(s) 394, 646, 682, 757, 763
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 1 cut(s) 385
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 1 cut(s) 382
BstMWI GCNNNNNNNGC 3 cut(s) 11, 373, 566
BstNI CCWGG 1 cut(s) 502
BstNSI RCATGY 1 cut(s) 443
BstPI GGTNACC 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 500
BstSLI GKGCMC 2 cut(s) 159, 313
BstV1I GCAGC 2 cut(s) 264, 360
BstV2I GAAGAC 2 cut(s) 51, 541
BsuRI GGCC 3 cut(s) 5, 157, 259
BtsCI GGATG 5 cut(s) 394, 646, 682, 757, 763
BtsIMutI CAGTG 1 cut(s) 174
BveI ACCTGC 1 cut(s) 722
CciI TCATGA 1 cut(s) 430
CfoI GCGC 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 3 cut(s) 155, 156, 257
CseI GACGC 3 cut(s) 341, 608, 809
Csp6I GTAC 2 cut(s) 100, 735
CviAII CATG 3 cut(s) 431, 440, 724
CviQI GTAC 2 cut(s) 100, 735
DdeI CTNAG 7 cut(s) 138, 148, 237, 321, 444, 515, 711
DpnI GATC 1 cut(s) 384
DpnII GATC 1 cut(s) 382
Eam1104I CTCTTC 4 cut(s) 336, 488, 665, 741
EarI CTCTTC 4 cut(s) 336, 488, 665, 741
Ecl136II GAGCTC 1 cut(s) 137
Eco24I GRGCYC 2 cut(s) 139, 159
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 4 cut(s) 288, 353, 383, 765
Eco91I GGTNACC 1 cut(s) 43
EcoICRI GAGCTC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 43
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 500
EcoT38I GRGCYC 2 cut(s) 139, 159
FaeI CATG 3 cut(s) 434, 443, 727
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FatI CATG 3 cut(s) 430, 439, 723
Fnu4HI GCNGC 2 cut(s) 278, 374
FokI GGATG 5 cut(s) 401, 653, 689, 764, 770
FriOI GRGCYC 2 cut(s) 139, 159
Fsp4HI GCNGC 2 cut(s) 278, 374
FspBI CTAG 4 cut(s) 485, 539, 836, 845
GlaI GCGC 1 cut(s) 404
GluI GCNGC 2 cut(s) 278, 374
HaeIII GGCC 3 cut(s) 5, 157, 259
HapII CCGG 2 cut(s) 29, 48
HgaI GACGC 3 cut(s) 341, 608, 809
HhaI GCGC 1 cut(s) 405
Hin1I GRCGYC 1 cut(s) 600
Hin1II CATG 3 cut(s) 434, 443, 727
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HindIII AAGCTT 2 cut(s) 365, 477
HinfI GANTC 3 cut(s) 427, 648, 740
HpaII CCGG 2 cut(s) 29, 48
HphI GGTGA 2 cut(s) 37, 676
Hpy166II GTNNAC 2 cut(s) 799, 830
Hpy188I TCNGA 8 cut(s) 36, 66, 85, 141, 363, 426, 582, 745
Hpy188III TCNNGA 6 cut(s) 266, 299, 386, 431, 622, 710
Hpy8I GTNNAC 2 cut(s) 799, 830
HpyAV CCTTC 5 cut(s) 114, 156, 205, 340, 457
HpyCH4III ACNGT 1 cut(s) 288
HpyCH4IV ACGT 1 cut(s) 576
HpyCH4V TGCA 3 cut(s) 123, 376, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 373, 566
HpyF3I CTNAG 7 cut(s) 138, 148, 237, 321, 444, 515, 711
HpySE526I ACGT 1 cut(s) 576
Hsp92I GRCGYC 1 cut(s) 600
Hsp92II CATG 3 cut(s) 434, 443, 727
HspAI GCGC 1 cut(s) 403
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 1 cut(s) 382
LmnI GCTCC 1 cut(s) 709
Lsp1109I GCAGC 2 cut(s) 264, 360
LweI GCATC 1 cut(s) 667
MaeI CTAG 4 cut(s) 485, 539, 836, 845
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 1 cut(s) 43
MalI GATC 1 cut(s) 384
MboI GATC 1 cut(s) 382
MhlI GDGCHC 3 cut(s) 139, 159, 313
MluCI AATT 3 cut(s) 86, 356, 471
MlyI GAGTC 1 cut(s) 657
MmeI TCCRAC 1 cut(s) 612
MroXI GAANNNNTTC 1 cut(s) 480
MseI TTAA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 2 cut(s) 29, 48
MspR9I CCNGG 1 cut(s) 502
Mva1269I GAATGC 2 cut(s) 534, 733
MvaI CCWGG 1 cut(s) 502
MwoI GCNNNNNNNGC 3 cut(s) 11, 373, 566
NdeII GATC 1 cut(s) 382
NlaIII CATG 3 cut(s) 434, 443, 727
NlaIV GGNNCC 3 cut(s) 68, 101, 157
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 443
PagI TCATGA 1 cut(s) 430
PcsI WCGNNNNNNNCGW 1 cut(s) 573
PctI GAATGC 2 cut(s) 534, 733
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 2 cut(s) 427, 740
PflMI CCANNNNNTGG 1 cut(s) 507
PkrI GCNGC 2 cut(s) 279, 375
PleI GAGTC 1 cut(s) 656
PpsI GAGTC 1 cut(s) 656
Psp124BI GAGCTC 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 500
PspEI GGTNACC 1 cut(s) 43
PspGI CCWGG 1 cut(s) 500
PspN4I GGNNCC 3 cut(s) 68, 101, 157
PspOMI GGGCCC 1 cut(s) 155
PspPI GGNCC 3 cut(s) 155, 156, 257
PsrI GAACNNNNNNTAC 2 cut(s) 557, 589
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 2 cut(s) 101, 736
RsaNI GTAC 2 cut(s) 100, 735
SacI GAGCTC 1 cut(s) 139
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 2 cut(s) 278, 374
Sau3AI GATC 1 cut(s) 382
Sau96I GGNCC 3 cut(s) 155, 156, 257
SchI GAGTC 1 cut(s) 657
ScrFI CCNGG 1 cut(s) 502
SduI GDGCHC 3 cut(s) 139, 159, 313
SfaNI GCATC 1 cut(s) 667
SpeI ACTAGT 1 cut(s) 844
Sse9I AATT 3 cut(s) 86, 356, 471
SsiI CCGC 1 cut(s) 329
SspMI CTAG 4 cut(s) 485, 539, 836, 845
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 500
TaaI ACNGT 1 cut(s) 288
TaiI ACGT 1 cut(s) 579
TaqI TCGA 1 cut(s) 290
TasI AATT 3 cut(s) 86, 356, 471
TfiI GAWTC 2 cut(s) 427, 740
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 2 cut(s) 277, 373
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 8 cut(s) 354, 369, 419, 599, 657, 683, 740, 792
TspGWI ACGGA 1 cut(s) 752
TspRI CASTG 1 cut(s) 174
Van91I CCANNNNNTGG 1 cut(s) 507
XapI RAATTY 2 cut(s) 86, 356
XceI RCATGY 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 4 cut(s) 485, 539, 836, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.