MD13G1149000.v1.1

Ribosomal protein S6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013271
Physical Location & Seq
Forward (+)
11711496 .. 11713795
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1149000.v1.1.491

Sequence Viewer

Length: 882 bp
ATGGGGGCACTCACATACTCCTTCTCTGGCGTTTCTCTCACCCCAACGGCTCTAAAACCGGTCGGCACGAATGGGTCCTCCTCCAGAAACCAAAATTCGAGCTTGAAATTCTCAGATGGGTACTCGTCGTTTTCTTCTCGCCGGCCATTTCCCACTCAGAAGCTCTCAGTCGGAGTGAGAAGGTCGGTGATTGTTGGTGCGAGGAAGAACAGCAACAAGGAGAAGAATAAGGAAGACAATCACAGCTTTGCATCGAAACCGGATGAGGCCACTGGGCCTTTCCCTGAATCTGTGCTGCTTAGACAGAAGAAAGTTCAGGAAGATGGTAATCTTGTCCCCGAGTTTGCTGATGCCGAAGAAGAGAAGGTGTATGAATTTTTGAAGCTTCAGCTGCAAAGCGATTTGAATGAGGAACGAATGCGCCACTATGAACTGGTTTATCTGATTCATGAGAAGCATGCGGAAGAGGTTGGGAGTGTCAAGGAGAAAATGGAAGAGTTTCTAGGGCAGAAAAAAGCCAGGATATGGAGAGTGAGTGATTGGGGAATGCGAAGACTAGCATACCAAATAAAGAAAGCGAAGAACGCTTACTATGTTTTGATGAACTTTGAGATGGAAGCCAAATGGATAAATGACTTCAAGACCATGCTGGACAAGGATGAGAGAGTCATTCGGCATCTTGTGATCAAGAGGGACGAGGCAATCACAGAAGACTGCCCTCCTCCTCCTGAGTTTCACACCCTCGGTGGAGGTACAGATTCTGATGAAGAAGAAGAGGATGATATGGAGTATGATGACGAAGAATATGAAGATGAAGATGGGATTATTATTGTCGACGCTGACAATGATGATATAGAAGAAAGGGTAGAACAACTAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S6

Protein Analysis

294

Amino Acids

33.73

Weight (kDa)

4.83

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S6 PF01250 142 - 230 1.3e-24 Ribosomal protein S6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17170
fragaria_vesca FvH4_4g20660
malus_domestica MD13G1149000.v1.1 MD16G1149200.v1.1
prunus_persica Prupe.1G113000_v2.0.a1
pyrus_communis pycom13g12820 pycom16g12750
rosa_chinensis RchiOBHm_Chr4g0426061
rosa_laevigata RLG00000007320
rosa_multiflora Rmu_ssc0000281.1_g000002 Rmu_ssc0000281.1_g000007
rosa_roxburghii Rroxscaffold_5G00368060
rosa_rugosa Rorug04G0208200
rosa_samantha Rh4AG265200 Rh4BG271300 Rh4CG284300 Rh4DG267600
rosa_wichuraiana Rw4G022930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 525
AccI GTMKAC 1 cut(s) 834
AciI CCGC 1 cut(s) 461
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 3 cut(s) 94, 107, 374
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 2 cut(s) 122, 754
AfiI CCNNNNNNNGG 1 cut(s) 525
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 4 cut(s) 106, 382, 406, 640
AhlI ACTAGT 1 cut(s) 874
AjnI CCWGG 1 cut(s) 518
AluBI AGCT 5 cut(s) 102, 163, 246, 385, 391
AluI AGCT 5 cut(s) 102, 163, 246, 385, 391
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 3 cut(s) 143, 267, 275
ApeKI GCWGC 2 cut(s) 295, 391
ApoI RAATTY 3 cut(s) 94, 107, 374
AsiGI ACCGGT 1 cut(s) 58
Asp700I GAANNNNTTC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 75, 275
AsuHPI GGTGA 2 cut(s) 31, 199
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 1 cut(s) 75
BaeGI GKGCMC 1 cut(s) 10
BarI GAAGNNNNNNTAC 2 cut(s) 572, 604
BbsI GAAGAC 3 cut(s) 240, 559, 717
BbvI GCAGC 2 cut(s) 282, 378
BccI CCATC 4 cut(s) 110, 317, 607, 812
BceAI ACGGC 1 cut(s) 63
BciT130I CCWGG 1 cut(s) 520
BclI TGATCA 1 cut(s) 684
BcuI ACTAGT 1 cut(s) 874
BfaI CTAG 3 cut(s) 503, 557, 875
BisI GCNGC 2 cut(s) 296, 392
BlsI GCNGC 2 cut(s) 297, 393
Bme1390I CCNGG 1 cut(s) 520
Bme18I GGWCC 1 cut(s) 75
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 75, 275
BmiI GGNNCC 1 cut(s) 76
BmrFI CCNGG 1 cut(s) 520
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 260, 340, 685
BmuI ACTGGG 1 cut(s) 282
BpiI GAAGAC 3 cut(s) 240, 559, 717
BpmI CTGGAG 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 742
BsaWI WCCGGW 2 cut(s) 58, 259
BsaXI ACNNNNNCTCC 2 cut(s) 520, 550
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse118I RCCGGY 2 cut(s) 58, 141
Bse1I ACTGG 2 cut(s) 277, 438
Bse8I GATNNNNATC 1 cut(s) 327
BseBI CCWGG 1 cut(s) 520
BseDI CCNNGG 1 cut(s) 742
BseGI GGATG 3 cut(s) 268, 664, 784
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMII CTCAG 4 cut(s) 126, 170, 180, 720
BseNI ACTGG 2 cut(s) 277, 438
BseRI GAGGAG 3 cut(s) 70, 711, 714
BseSI GKGCMC 1 cut(s) 10
BseXI GCAGC 2 cut(s) 282, 378
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 3 cut(s) 145, 269, 277
BshTI ACCGGT 1 cut(s) 58
BsiEI CGRYCG 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 3 cut(s) 59, 142, 260
BslFI GGGAC 2 cut(s) 320, 707
BslI CCNNNNNNNGG 1 cut(s) 525
BsmFI GGGAC 2 cut(s) 320, 707
BsmI GAATGC 2 cut(s) 423, 552
BsnI GGCC 3 cut(s) 145, 269, 277
BsoBI CYCGRG 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 1 cut(s) 684
BspACI CCGC 1 cut(s) 461
BspANI GGCC 3 cut(s) 145, 269, 277
BspCNI CTCAG 4 cut(s) 125, 169, 179, 721
BspHI TCATGA 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 76
BsrFI RCCGGY 2 cut(s) 58, 141
BsrI ACTGG 2 cut(s) 277, 438
BssAI RCCGGY 2 cut(s) 58, 141
BssECI CCNNGG 1 cut(s) 742
BssMI GATC 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 520
Bst6I CTCTTC 4 cut(s) 354, 459, 489, 768
BstC8I GCNNGC 2 cut(s) 143, 459
BstDEI CTNAG 5 cut(s) 112, 156, 166, 299, 729
BstF5I GGATG 3 cut(s) 268, 664, 784
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 1 cut(s) 687
BstMBI GATC 1 cut(s) 684
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 2 cut(s) 391, 584
BstNI CCWGG 1 cut(s) 520
BstNSI RCATGY 1 cut(s) 461
BstSCI CCNGG 1 cut(s) 518
BstSLI GKGCMC 1 cut(s) 10
BstV1I GCAGC 2 cut(s) 282, 378
BstV2I GAAGAC 3 cut(s) 240, 559, 717
BsuRI GGCC 3 cut(s) 145, 269, 277
BtsCI GGATG 3 cut(s) 268, 664, 784
BtsIMutI CAGTG 1 cut(s) 270
Cac8I GCNNGC 2 cut(s) 143, 459
CaiI CAGNNNCTG 1 cut(s) 761
CciI TCATGA 1 cut(s) 448
CfoI GCGC 1 cut(s) 423
Cfr10I RCCGGY 2 cut(s) 58, 141
Cfr13I GGNCC 2 cut(s) 75, 275
CseI GACGC 1 cut(s) 845
Csp6I GTAC 2 cut(s) 121, 753
CspAI ACCGGT 1 cut(s) 58
CviAII CATG 3 cut(s) 449, 458, 646
CviQI GTAC 2 cut(s) 121, 753
DdeI CTNAG 5 cut(s) 112, 156, 166, 299, 729
DpnI GATC 1 cut(s) 686
DpnII GATC 1 cut(s) 684
EaeI YGGCCR 1 cut(s) 143
Eam1104I CTCTTC 4 cut(s) 354, 459, 489, 768
EarI CTCTTC 4 cut(s) 354, 459, 489, 768
Eco47I GGWCC 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 518
FaeI CATG 3 cut(s) 452, 461, 649
FaqI GGGAC 2 cut(s) 320, 707
FatI CATG 3 cut(s) 448, 457, 645
FbaI TGATCA 1 cut(s) 684
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 2 cut(s) 296, 392
FokI GGATG 3 cut(s) 275, 671, 791
Fsp4HI GCNGC 2 cut(s) 296, 392
FspBI CTAG 3 cut(s) 503, 557, 875
GlaI GCGC 1 cut(s) 422
GluI GCNGC 2 cut(s) 296, 392
GsuI CTGGAG 1 cut(s) 67
HaeIII GGCC 3 cut(s) 145, 269, 277
HapII CCGG 3 cut(s) 59, 142, 260
HgaI GACGC 1 cut(s) 845
HhaI GCGC 1 cut(s) 423
Hin1II CATG 3 cut(s) 452, 461, 649
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HincII GTYRAC 1 cut(s) 835
HindII GTYRAC 1 cut(s) 835
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 4 cut(s) 287, 445, 666, 758
HpaII CCGG 3 cut(s) 59, 142, 260
HphI GGTGA 2 cut(s) 31, 199
Hpy166II GTNNAC 1 cut(s) 835
Hpy188I TCNGA 5 cut(s) 115, 159, 173, 444, 763
Hpy188III TCNNGA 6 cut(s) 84, 317, 449, 640, 688, 728
Hpy8I GTNNAC 1 cut(s) 835
Hpy99I CGWCG 2 cut(s) 130, 839
HpyAV CCTTC 3 cut(s) 31, 174, 358
HpyCH4V TGCA 2 cut(s) 251, 394
HpyF10VI GCNNNNNNNGC 2 cut(s) 391, 584
HpyF3I CTNAG 5 cut(s) 112, 156, 166, 299, 729
Hsp92II CATG 3 cut(s) 452, 461, 649
HspAI GCGC 1 cut(s) 421
KroI GCCGGC 1 cut(s) 141
KroNI GCCGGC 1 cut(s) 143
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 1 cut(s) 684
Lsp1109I GCAGC 2 cut(s) 282, 378
LweI GCATC 3 cut(s) 260, 340, 685
MaeI CTAG 3 cut(s) 503, 557, 875
MalI GATC 1 cut(s) 686
MboI GATC 1 cut(s) 684
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 3 cut(s) 94, 107, 374
MlyI GAGTC 1 cut(s) 675
MmeI TCCRAC 1 cut(s) 151
MroNI GCCGGC 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 3 cut(s) 59, 142, 260
MspR9I CCNGG 1 cut(s) 520
Mva1269I GAATGC 2 cut(s) 423, 552
MvaI CCWGG 1 cut(s) 520
MwoI GCNNNNNNNGC 2 cut(s) 391, 584
NaeI GCCGGC 1 cut(s) 143
NdeII GATC 1 cut(s) 684
NgoMIV GCCGGC 1 cut(s) 141
NlaIII CATG 3 cut(s) 452, 461, 649
NlaIV GGNNCC 1 cut(s) 76
NspI RCATGY 1 cut(s) 461
PaeI GCATGC 1 cut(s) 461
PagI TCATGA 1 cut(s) 448
PctI GAATGC 2 cut(s) 423, 552
PdiI GCCGGC 1 cut(s) 143
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 3 cut(s) 287, 445, 758
PflMI CCANNNNNTGG 1 cut(s) 525
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 2 cut(s) 297, 393
PleI GAGTC 1 cut(s) 674
PpsI GAGTC 1 cut(s) 674
PpuMI RGGWCCY 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 518
PspGI CCWGG 1 cut(s) 518
PspN4I GGNNCC 1 cut(s) 76
PspPI GGNCC 2 cut(s) 75, 275
PspPPI RGGWCCY 1 cut(s) 75
PstNI CAGNNNCTG 1 cut(s) 761
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 2 cut(s) 122, 754
RsaNI GTAC 2 cut(s) 121, 753
SalI GTCGAC 1 cut(s) 833
SatI GCNGC 2 cut(s) 296, 392
Sau3AI GATC 1 cut(s) 684
Sau96I GGNCC 2 cut(s) 75, 275
SchI GAGTC 1 cut(s) 675
ScrFI CCNGG 1 cut(s) 520
SduI GDGCHC 1 cut(s) 10
SetI ASST 9 cut(s) 104, 165, 185, 248, 369, 387, 393, 471, 754
SfaNI GCATC 3 cut(s) 260, 340, 685
SinI GGWCC 1 cut(s) 75
SpeI ACTAGT 1 cut(s) 874
SphI GCATGC 1 cut(s) 461
Sse9I AATT 3 cut(s) 94, 107, 374
SsiI CCGC 1 cut(s) 461
SspMI CTAG 3 cut(s) 503, 557, 875
StyD4I CCNGG 1 cut(s) 518
TaqI TCGA 3 cut(s) 98, 254, 834
TasI AATT 3 cut(s) 94, 107, 374
TfiI GAWTC 3 cut(s) 287, 445, 758
TscAI CASTG 1 cut(s) 277
TseI GCWGC 2 cut(s) 295, 391
TspDTI ATGAA 7 cut(s) 387, 437, 444, 617, 780, 822, 828
TspRI CASTG 1 cut(s) 277
Van91I CCANNNNNTGG 1 cut(s) 525
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 3 cut(s) 94, 107, 374
XceI RCATGY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 3 cut(s) 503, 557, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.