FvH4_4g21130

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013145
Physical Location & Seq
Forward (+)
24257037 .. 24258399
1363 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21130.t1

Sequence Viewer

Length: 888 bp
ATGAGGCCTCTCTCTAAACCACTCACCATCATCAACTTCACACTCCTCCTCTCACTTCTCCCTACCCTAACCCTCTCCCAATGCCGCGACTCCTGCGGCGACATCTCCATAAACTACCCTTTTGGCCTCGACGACGGCTGCGGTGCCCCGCAGTTCCGCCGCATGCTCAACTGCTCCACCGACCTCTTCTTCCTCACTCCCTCCGGCTCCTACAAAGTCGAGTCCATCAACTACGACAAGAAAACCATCGTCGTTTACGACCCCGCCATGTCCACCTGCTCCATCCTCCAGCCGCACCACGACTTCCTCATGACGGACATCCAGTCAGCCCTAATCCCTCCGACCTCCGACACCGTCTTTGCCCTCCTCAACTGCTCTATTGACTCCCCTGTCCTCAACCACTACAGCTACCTCTGCTTCAACTTCTCCTCTCACTCATGCGACGAGCTCTACGGCTCGTGCAACGCTTTTAGGGTTTTCCACATGATGGCCAACACTACTTCGCCGCCGTGCTGCTTTACGAGTTACGACACCGTTAAGTTCATGAGCATGAATGTCTTGGACTGCACGCATTACACGACGGTGACGAACACGGACGGGTTGAGTGGGATTGGGCCGCAGGATTGGGTTTATGGGATCAAGATGAGCTTCGCCGTGCCGGATTCCGGTTGCGACAGGTGTGCGGCGTCGGGTGGGACGTGCGGGTTTGATACCGAGACTGAAGGGCAGCTGTGTCTCTGCTCTAGTTCGTCGAATTATACTAGAGAATGTGGTTTAGGCAGTGTGACGGCGGGAGTGAACTGTGCAACCAGCAGCGTAGGCTTCCAGGCATTCTTTGTGATCGTGTTGATCTATAAGTTATTCAACAGTGTAGCACTTTTTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

296

Amino Acids

32.23

Weight (kDa)

4.81

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 87 1.9e-14 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17350 AT3G17350
fragaria_vesca FvH4_4g21130
malus_domestica MD16G1139400.v1.1
prunus_persica Prupe.1G108900_v2.0.a1
pyrus_communis pycom16g12380
rosa_chinensis RchiOBHm_Chr4g0426711
rosa_laevigata RLG00000007284
rosa_multiflora Rmu_co8392541.1_g000001 Rmu_sc0010934.1_g000002 Rmu_sc0021908.1_g000002
rosa_roxburghii Rroxscaffold_5G00368680
rosa_rugosa Rorug04G0214400
rosa_samantha Rh4AG269400 Rh4BG275700 Rh4CG290200 Rh4DG272700
rosa_wichuraiana Rw4G023370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
AasI GACNNNNNNGTC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 487
AccII CGCG 1 cut(s) 87
AclWI GGATC 1 cut(s) 644
AcoI YGGCCR 1 cut(s) 489
AcuI CTGAAG 1 cut(s) 741
AcyI GRCGYC 1 cut(s) 686
AfiI CCNNNNNNNGG 3 cut(s) 313, 487, 665
AgsI TTSAA 2 cut(s) 421, 865
AhdI GACNNNNNGTC 1 cut(s) 322
AjiI CACGTC 1 cut(s) 699
AjnI CCWGG 1 cut(s) 825
AleI CACNNNNGTG 1 cut(s) 581
AluBI AGCT 4 cut(s) 408, 448, 648, 730
AluI AGCT 4 cut(s) 408, 448, 648, 730
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 710, 740
AlwI GGATC 1 cut(s) 644
AoxI GGCC 4 cut(s) 5, 124, 489, 614
ApeKI GCWGC 4 cut(s) 138, 513, 727, 813
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AspS9I GGNCC 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 16, 595
BaeGI GKGCMC 1 cut(s) 148
BalI TGGCCA 1 cut(s) 491
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 450
BauI CACGAG 1 cut(s) 457
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 4 cut(s) 125, 500, 739, 825
BccI CCATC 5 cut(s) 35, 233, 254, 290, 481
BceAI ACGGC 5 cut(s) 151, 469, 493, 638, 804
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciT130I CCWGG 1 cut(s) 827
BcoDI GTCTC 2 cut(s) 710, 740
BfaI CTAG 2 cut(s) 744, 762
BfmI CTRYAG 1 cut(s) 403
BfuAI ACCTGC 1 cut(s) 284
Bme1390I CCNGG 1 cut(s) 827
BmeRI GACNNNNNGTC 1 cut(s) 322
BmgBI CACGTC 1 cut(s) 699
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 2 cut(s) 145, 208
BmrFI CCNGG 1 cut(s) 827
BpmI CTGGAG 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 686
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 487, 665
Bse1I ACTGG 1 cut(s) 322
BseBI CCWGG 1 cut(s) 827
BseGI GGATG 2 cut(s) 282, 318
BseLI CCNNNNNNNGG 3 cut(s) 313, 487, 665
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 4 cut(s) 35, 38, 356, 418
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 4 cut(s) 125, 500, 739, 825
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 4 cut(s) 7, 126, 491, 616
BshNI GGYRCC 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 3 cut(s) 204, 659, 666
BslFI GGGAC 1 cut(s) 709
BslI CCNNNNNNNGG 3 cut(s) 313, 487, 665
BsmAI GTCTC 2 cut(s) 710, 740
BsmFI GGGAC 1 cut(s) 709
BsmI GAATGC 1 cut(s) 830
BsnI GGCC 4 cut(s) 7, 126, 491, 616
Bsp1286I GDGCHC 2 cut(s) 148, 450
Bsp143I GATC 3 cut(s) 636, 840, 849
BspANI GGCC 4 cut(s) 7, 126, 491, 616
BspFNI CGCG 1 cut(s) 87
BspHI TCATGA 2 cut(s) 309, 543
BspLI GGNNCC 2 cut(s) 145, 208
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 1 cut(s) 644
BspT107I GGYRCC 1 cut(s) 143
BsrI ACTGG 1 cut(s) 322
BssMI GATC 3 cut(s) 636, 840, 849
BssNI GRCGYC 1 cut(s) 686
BssSI CACGAG 1 cut(s) 457
Bst2BI CACGAG 1 cut(s) 457
Bst2UI CCWGG 1 cut(s) 827
Bst4CI ACNGT 5 cut(s) 355, 535, 583, 803, 869
Bst6I CTCTTC 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 686
BstC8I GCNNGC 2 cut(s) 164, 569
BstF5I GGATG 2 cut(s) 282, 318
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 3 cut(s) 639, 843, 852
BstMAI GTCTC 2 cut(s) 710, 740
BstMBI GATC 3 cut(s) 636, 840, 849
BstMWI GCNNNNNNNGC 3 cut(s) 93, 414, 819
BstNI CCWGG 1 cut(s) 827
BstNSI RCATGY 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 825
BstSFI CTRYAG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 148
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 4 cut(s) 125, 500, 739, 825
BsuRI GGCC 4 cut(s) 7, 126, 491, 616
BtrI CACGTC 1 cut(s) 699
BtsCI GGATG 2 cut(s) 282, 318
BtsI GCAGTG 1 cut(s) 787
BtsIMutI CAGTG 2 cut(s) 787, 874
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 2 cut(s) 164, 569
CciI TCATGA 2 cut(s) 309, 543
Cfr13I GGNCC 1 cut(s) 614
CseI GACGC 1 cut(s) 675
CviAII CATG 7 cut(s) 163, 268, 310, 438, 484, 544, 550
DpnI GATC 3 cut(s) 638, 842, 851
DpnII GATC 3 cut(s) 636, 840, 849
DrdI GACNNNNNNGTC 1 cut(s) 389
DriI GACNNNNNGTC 1 cut(s) 322
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 1 cut(s) 191
Eam1105I GACNNNNNGTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 191
EciI GGCGGA 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 448
Eco147I AGGCCT 1 cut(s) 7
Eco24I GRGCYC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 741
EcoICRI GAGCTC 1 cut(s) 448
EcoRII CCWGG 1 cut(s) 825
EcoT38I GRGCYC 1 cut(s) 450
FaeI CATG 7 cut(s) 166, 271, 313, 441, 487, 547, 553
FalI AAGNNNNNCTT 2 cut(s) 632, 664
FaqI GGGAC 1 cut(s) 709
FatI CATG 7 cut(s) 162, 267, 309, 437, 483, 543, 549
FauI CCCGC 4 cut(s) 156, 271, 695, 784
FokI GGATG 2 cut(s) 269, 305
FriOI GRGCYC 1 cut(s) 450
FspBI CTAG 2 cut(s) 744, 762
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 4 cut(s) 7, 126, 491, 616
HapII CCGG 3 cut(s) 204, 659, 666
HgaI GACGC 1 cut(s) 675
Hin1I GRCGYC 1 cut(s) 686
Hin1II CATG 7 cut(s) 166, 271, 313, 441, 487, 547, 553
HinfI GANTC 4 cut(s) 89, 221, 383, 662
HpaII CCGG 3 cut(s) 204, 659, 666
HphI GGTGA 2 cut(s) 16, 595
Hpy166II GTNNAC 3 cut(s) 256, 273, 799
Hpy188I TCNGA 2 cut(s) 342, 349
Hpy188III TCNNGA 3 cut(s) 310, 544, 640
Hpy8I GTNNAC 3 cut(s) 256, 273, 799
Hpy99I CGWCG 7 cut(s) 134, 137, 254, 446, 583, 691, 754
HpyAV CCTTC 1 cut(s) 716
HpyCH4III ACNGT 5 cut(s) 355, 535, 583, 803, 869
HpyCH4IV ACGT 1 cut(s) 698
HpyCH4V TGCA 3 cut(s) 462, 567, 806
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 414, 819
HpySE526I ACGT 1 cut(s) 698
Hsp92I GRCGYC 1 cut(s) 686
Hsp92II CATG 7 cut(s) 166, 271, 313, 441, 487, 547, 553
Kzo9I GATC 3 cut(s) 636, 840, 849
LmnI GCTCC 3 cut(s) 179, 212, 284
Lsp1109I GCAGC 4 cut(s) 125, 500, 739, 825
MaeI CTAG 2 cut(s) 744, 762
MaeII ACGT 1 cut(s) 698
MaeIII GTNAC 3 cut(s) 524, 583, 784
MalI GATC 3 cut(s) 638, 842, 851
MboI GATC 3 cut(s) 636, 840, 849
MboII GAAGA 2 cut(s) 178, 181
MhlI GDGCHC 2 cut(s) 148, 450
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 1 cut(s) 754
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 3 cut(s) 83, 230, 377
MmeI TCCRAC 2 cut(s) 365, 372
Mox20I TGGCCA 1 cut(s) 491
MscI TGGCCA 1 cut(s) 491
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 2 cut(s) 548, 581
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 730
MspI CCGG 3 cut(s) 204, 659, 666
MspR9I CCNGG 1 cut(s) 827
Mva1269I GAATGC 1 cut(s) 830
MvaI CCWGG 1 cut(s) 827
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 93, 414, 819
NdeII GATC 3 cut(s) 636, 840, 849
NlaIII CATG 7 cut(s) 166, 271, 313, 441, 487, 547, 553
NlaIV GGNNCC 2 cut(s) 145, 208
NmuCI GTSAC 2 cut(s) 583, 784
NspI RCATGY 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 581
PaeI GCATGC 1 cut(s) 166
PagI TCATGA 2 cut(s) 309, 543
PaqCI CACCTGC 1 cut(s) 284
PceI AGGCCT 1 cut(s) 7
PcsI WCGNNNNNNNCGW 4 cut(s) 255, 575, 584, 695
PctI GAATGC 1 cut(s) 830
PfeI GAWTC 1 cut(s) 662
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 487
PleI GAGTC 3 cut(s) 83, 229, 377
PpsI GAGTC 3 cut(s) 83, 229, 377
Psp124BI GAGCTC 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 825
PspGI CCWGG 1 cut(s) 825
PspN4I GGNNCC 2 cut(s) 145, 208
PspPI GGNCC 1 cut(s) 614
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 730
RseI CAYNNNNRTG 2 cut(s) 548, 581
SacI GAGCTC 1 cut(s) 450
SaqAI TTAA 1 cut(s) 537
Sau3AI GATC 3 cut(s) 636, 840, 849
Sau96I GGNCC 1 cut(s) 614
SchI GAGTC 3 cut(s) 83, 230, 377
ScrFI CCNGG 1 cut(s) 827
SduI GDGCHC 2 cut(s) 148, 450
SfcI CTRYAG 1 cut(s) 403
SmiMI CAYNNNNRTG 2 cut(s) 548, 581
SphI GCATGC 1 cut(s) 166
Sse9I AATT 1 cut(s) 754
SseBI AGGCCT 1 cut(s) 7
SspMI CTAG 2 cut(s) 744, 762
SstI GAGCTC 1 cut(s) 450
StuI AGGCCT 1 cut(s) 7
StyD4I CCNGG 1 cut(s) 825
TaaI ACNGT 5 cut(s) 355, 535, 583, 803, 869
TaiI ACGT 1 cut(s) 701
TaqI TCGA 3 cut(s) 129, 219, 752
TasI AATT 1 cut(s) 754
TauI GCSGC 7 cut(s) 87, 99, 162, 295, 508, 619, 686
TfiI GAWTC 1 cut(s) 662
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 2 cut(s) 787, 874
TseFI GTSAC 2 cut(s) 583, 784
TseI GCWGC 4 cut(s) 138, 513, 727, 813
Tsp45I GTSAC 2 cut(s) 583, 784
TspDTI ATGAA 2 cut(s) 532, 566
TspGWI ACGGA 2 cut(s) 329, 608
TspRI CASTG 2 cut(s) 787, 874
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 487
XceI RCATGY 1 cut(s) 166
XspI CTAG 2 cut(s) 744, 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.