Rw4G023370

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013145
Physical Location & Seq
Forward (+)
48996533 .. 48997615
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G023370.1

Sequence Viewer

Length: 894 bp
ATGAGGTCTCTCTCAAAACCAGTCACCATCATCAACTTCATAGTAGTACTCCTCTCACAGCTCCCTACCCTAACTCTCTCCCAATGCCGCGACTCCTGCGGCGACATCCCCATAAACTACCCTTTTGCCCTCGATGATGGCTGCGGCGCCCCGCAGTTCCGCCGCATGCTCAATTGCTCCACCGACCTCTTCTTCCTCACCCCCTCCGGCTCCTACAAAGTCGAGTCCATCAATTACGACAAGAAAACCATCGTCGTTTACGACCCCGCCATGTCCACCTGCTCCATCCTCCAGCCCCACCACGACTTCCTCATGACCGATATCCAGTCGGCCCTAATTCCCCCGACCTCTGACACCGTCTTTGCCCTCCTCAACTGCTCTATTGACTCCCCTGTCCTCAACCACTACAGTTACCTCTGCTTCAACTTCTCTTCTCACTCCTGCGACGAGCTCTACGGCTCGTGCAACGCGTTTAGGGTTTTCCACATGGTTGCCAACACTACTTCGCCGCCGTGCTGCTTTACAGGTTACGACACCGTTAAGTTCATGAGCATGAACATCTTGGACTGCACGCATTACACAACGGTGACGAACACGGATGAGTTGAGGGGGATTGGACCGCAGGATTGGGTTTATGGGATCAAGATGACCTTCGCCGTGCCGGATTCCGGTTGCAACAGGTGTGCGATGTCCGGTGGCACGTGCGGGTTTGATACAGAGACCGAAGGGGCACTGTGTCTTTGCTCGAGTTCGTCGAATTATACTAGAGAATGTGCTGTAGGCAGTGTCACGGCGGGAGTGAACAACTGTGCAACCAGCAGCATATTCTTGCAATCAATTTTTGTGATCGTGTTGATCCATAATTTATTCAACCTAGTATTTTTGTCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

297

Amino Acids

32.58

Weight (kDa)

4.94

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 29 - 88 1.4e-13 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 213 - 250 5e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17350 AT3G17350
fragaria_vesca FvH4_4g21130
malus_domestica MD16G1139400.v1.1
prunus_persica Prupe.1G108900_v2.0.a1
pyrus_communis pycom16g12380
rosa_chinensis RchiOBHm_Chr4g0426711
rosa_laevigata RLG00000007284
rosa_multiflora Rmu_co8392541.1_g000001 Rmu_sc0010934.1_g000002 Rmu_sc0021908.1_g000002
rosa_roxburghii Rroxscaffold_5G00368680
rosa_rugosa Rorug04G0214400
rosa_samantha Rh4AG269400 Rh4BG275700 Rh4CG290200 Rh4DG272700
rosa_wichuraiana Rw4G023370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 287
AasI GACNNNNNNGTC 1 cut(s) 392
Acc36I ACCTGC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 146
AccII CGCG 2 cut(s) 90, 470
AclWI GGATC 2 cut(s) 647, 850
AcvI CACGTG 1 cut(s) 702
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 1 cut(s) 668
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 2 cut(s) 424, 871
AleI CACNNNNGTG 1 cut(s) 584
AluBI AGCT 2 cut(s) 61, 451
AluI AGCT 2 cut(s) 61, 451
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 2 cut(s) 12, 713
AlwI GGATC 2 cut(s) 647, 850
Ama87I CYCGRG 1 cut(s) 745
AoxI GGCC 1 cut(s) 330
ApeKI GCWGC 3 cut(s) 141, 516, 819
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 331, 617
AsuHPI GGTGA 3 cut(s) 16, 190, 598
AvaI CYCGRG 1 cut(s) 745
AvaII GGWCC 1 cut(s) 617
BaeGI GKGCMC 1 cut(s) 733
BanI GGYRCC 1 cut(s) 146
BanII GRGCYC 1 cut(s) 453
BauI CACGAG 1 cut(s) 460
BbrPI CACGTG 1 cut(s) 702
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 128, 503, 831
BccI CCATC 5 cut(s) 35, 131, 236, 257, 293
BceAI ACGGC 4 cut(s) 472, 496, 641, 807
BcoDI GTCTC 2 cut(s) 12, 713
BfaI CTAG 2 cut(s) 765, 875
BfmI CTRYAG 2 cut(s) 406, 777
BfoI RGCGCY 1 cut(s) 150
BfuAI ACCTGC 1 cut(s) 287
BisI GCNGC 8 cut(s) 88, 100, 142, 145, 163, 509, 517, 820
BlsI GCNGC 8 cut(s) 89, 101, 143, 146, 164, 510, 518, 821
BmcAI AGTACT 1 cut(s) 48
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 745
BmgT120I GGNCC 2 cut(s) 331, 617
BmiI GGNNCC 2 cut(s) 148, 211
BpmI CTGGAG 1 cut(s) 275
BsaAI YACGTR 1 cut(s) 702
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 2 cut(s) 12, 713
BsaWI WCCGGW 2 cut(s) 668, 692
Bsc4I CCNNNNNNNGG 1 cut(s) 668
Bse1I ACTGG 2 cut(s) 20, 325
BseGI GGATG 3 cut(s) 105, 285, 604
BseLI CCNNNNNNNGG 1 cut(s) 668
BseNI ACTGG 2 cut(s) 20, 325
BseRI GAGGAG 2 cut(s) 41, 359
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 3 cut(s) 128, 503, 831
BsgI GTGCAG 1 cut(s) 553
Bsh1236I CGCG 2 cut(s) 90, 470
BshFI GGCC 1 cut(s) 332
BshNI GGYRCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 453
BsiHKCI CYCGRG 1 cut(s) 745
BsiSI CCGG 4 cut(s) 207, 662, 669, 693
BslI CCNNNNNNNGG 1 cut(s) 668
BsmAI GTCTC 2 cut(s) 12, 713
BsnI GGCC 1 cut(s) 332
Bso31I GGTCTC 2 cut(s) 12, 713
BsoBI CYCGRG 1 cut(s) 745
Bsp1286I GDGCHC 2 cut(s) 453, 733
Bsp143I GATC 3 cut(s) 639, 846, 855
BspANI GGCC 1 cut(s) 332
BspFNI CGCG 2 cut(s) 90, 470
BspHI TCATGA 2 cut(s) 312, 546
BspLI GGNNCC 2 cut(s) 148, 211
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 2 cut(s) 647, 850
BspT107I GGYRCC 1 cut(s) 146
BspTNI GGTCTC 2 cut(s) 12, 713
BsrI ACTGG 2 cut(s) 20, 325
BssMI GATC 3 cut(s) 639, 846, 855
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 460
Bst2BI CACGAG 1 cut(s) 460
Bst4CI ACNGT 6 cut(s) 358, 410, 538, 586, 735, 809
Bst6I CTCTTC 2 cut(s) 194, 436
BstACI GRCGYC 1 cut(s) 147
BstBAI YACGTR 1 cut(s) 702
BstC8I GCNNGC 2 cut(s) 167, 572
BstF5I GGATG 3 cut(s) 105, 285, 604
BstFNI CGCG 2 cut(s) 90, 470
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 3 cut(s) 642, 849, 858
BstMAI GTCTC 2 cut(s) 12, 713
BstMBI GATC 3 cut(s) 639, 846, 855
BstMWI GCNNNNNNNGC 1 cut(s) 96
BstNSI RCATGY 1 cut(s) 169
BstSFI CTRYAG 2 cut(s) 406, 777
BstSLI GKGCMC 1 cut(s) 733
BstUI CGCG 2 cut(s) 90, 470
BstV1I GCAGC 3 cut(s) 128, 503, 831
BsuRI GGCC 1 cut(s) 332
BtgZI GCGATG 1 cut(s) 701
BtsCI GGATG 3 cut(s) 105, 285, 604
BtsI GCAGTG 1 cut(s) 790
BtsIMutI CAGTG 2 cut(s) 731, 790
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 2 cut(s) 167, 572
CciI TCATGA 2 cut(s) 312, 546
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 331, 617
Csp6I GTAC 1 cut(s) 47
CviAII CATG 6 cut(s) 166, 271, 313, 487, 547, 553
CviJI RGCY 7 cut(s) 61, 141, 210, 295, 332, 451, 459
CviKI_1 RGCY 7 cut(s) 61, 141, 210, 295, 332, 451, 459
CviQI GTAC 1 cut(s) 47
DinI GGCGCC 1 cut(s) 148
DpnI GATC 3 cut(s) 641, 848, 857
DpnII GATC 3 cut(s) 639, 846, 855
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 194, 436
EarI CTCTTC 2 cut(s) 194, 436
EciI GGCGGA 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 453
Eco31I GGTCTC 2 cut(s) 12, 713
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 617
Eco53kI GAGCTC 1 cut(s) 451
Eco72I CACGTG 1 cut(s) 702
Eco88I CYCGRG 1 cut(s) 745
EcoICRI GAGCTC 1 cut(s) 451
EcoRV GATATC 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 453
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
FaeI CATG 6 cut(s) 169, 274, 316, 490, 550, 556
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FatI CATG 6 cut(s) 165, 270, 312, 486, 546, 552
FauI CCCGC 4 cut(s) 159, 274, 698, 787
Fnu4HI GCNGC 8 cut(s) 88, 100, 142, 145, 163, 509, 517, 820
FokI GGATG 3 cut(s) 92, 272, 611
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 8 cut(s) 88, 100, 142, 145, 163, 509, 517, 820
FspBI CTAG 2 cut(s) 765, 875
GlaI GCGC 1 cut(s) 148
GluI GCNGC 8 cut(s) 88, 100, 142, 145, 163, 509, 517, 820
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 1 cut(s) 332
HapII CCGG 4 cut(s) 207, 662, 669, 693
HhaI GCGC 1 cut(s) 149
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 6 cut(s) 169, 274, 316, 490, 550, 556
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HinfI GANTC 4 cut(s) 92, 224, 386, 665
HpaII CCGG 4 cut(s) 207, 662, 669, 693
HphI GGTGA 3 cut(s) 16, 190, 598
Hpy166II GTNNAC 3 cut(s) 259, 276, 802
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 3 cut(s) 313, 547, 643
Hpy8I GTNNAC 3 cut(s) 259, 276, 802
Hpy99I CGWCG 3 cut(s) 257, 449, 757
HpyAV CCTTC 2 cut(s) 661, 719
HpyCH4III ACNGT 6 cut(s) 358, 410, 538, 586, 735, 809
HpyCH4IV ACGT 1 cut(s) 701
HpyCH4V TGCA 5 cut(s) 465, 570, 675, 812, 832
HpyF10VI GCNNNNNNNGC 1 cut(s) 96
HpySE526I ACGT 1 cut(s) 701
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 6 cut(s) 169, 274, 316, 490, 550, 556
HspAI GCGC 1 cut(s) 147
KasI GGCGCC 1 cut(s) 146
Kzo9I GATC 3 cut(s) 639, 846, 855
LmnI GCTCC 4 cut(s) 66, 182, 215, 287
Lsp1109I GCAGC 3 cut(s) 128, 503, 831
MaeI CTAG 2 cut(s) 765, 875
MaeII ACGT 1 cut(s) 701
MaeIII GTNAC 5 cut(s) 22, 410, 527, 586, 787
MalI GATC 3 cut(s) 641, 848, 857
MboI GATC 3 cut(s) 639, 846, 855
MboII GAAGA 3 cut(s) 181, 184, 423
MfeI CAATTG 1 cut(s) 172
MhlI GDGCHC 2 cut(s) 453, 733
MluCI AATT 7 cut(s) 172, 232, 336, 757, 837, 862, 889
MluI ACGCGT 1 cut(s) 468
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 3 cut(s) 86, 233, 380
MseI TTAA 2 cut(s) 540, 892
MslI CAYNNNNRTG 2 cut(s) 551, 584
MspI CCGG 4 cut(s) 207, 662, 669, 693
MunI CAATTG 1 cut(s) 172
MvnI CGCG 2 cut(s) 90, 470
MwoI GCNNNNNNNGC 1 cut(s) 96
NarI GGCGCC 1 cut(s) 147
NdeII GATC 3 cut(s) 639, 846, 855
NlaIII CATG 6 cut(s) 169, 274, 316, 490, 550, 556
NlaIV GGNNCC 2 cut(s) 148, 211
NmuCI GTSAC 3 cut(s) 22, 586, 787
NspI RCATGY 1 cut(s) 169
OliI CACNNNNGTG 1 cut(s) 584
PaeI GCATGC 1 cut(s) 169
PaeR7I CTCGAG 1 cut(s) 745
PagI TCATGA 2 cut(s) 312, 546
PaqCI CACCTGC 1 cut(s) 287
PcsI WCGNNNNNNNCGW 3 cut(s) 258, 467, 752
PfeI GAWTC 1 cut(s) 665
PflFI GACNNNGTC 1 cut(s) 356
PkrI GCNGC 8 cut(s) 89, 101, 143, 146, 164, 510, 518, 821
PleI GAGTC 3 cut(s) 86, 232, 380
PluTI GGCGCC 1 cut(s) 150
PmaCI CACGTG 1 cut(s) 702
PmlI CACGTG 1 cut(s) 702
PpsI GAGTC 3 cut(s) 86, 232, 380
Ppu21I YACGTR 1 cut(s) 702
Psp124BI GAGCTC 1 cut(s) 453
PspCI CACGTG 1 cut(s) 702
PspN4I GGNNCC 2 cut(s) 148, 211
PspPI GGNCC 2 cut(s) 331, 617
PspXI VCTCGAGB 1 cut(s) 745
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 2 cut(s) 551, 584
SacI GAGCTC 1 cut(s) 453
SaqAI TTAA 2 cut(s) 540, 892
SatI GCNGC 8 cut(s) 88, 100, 142, 145, 163, 509, 517, 820
Sau3AI GATC 3 cut(s) 639, 846, 855
Sau96I GGNCC 2 cut(s) 331, 617
ScaI AGTACT 1 cut(s) 48
SchI GAGTC 3 cut(s) 86, 233, 380
SduI GDGCHC 2 cut(s) 453, 733
SfcI CTRYAG 2 cut(s) 406, 777
SfoI GGCGCC 1 cut(s) 148
Sfr274I CTCGAG 1 cut(s) 745
SinI GGWCC 1 cut(s) 617
SlaI CTCGAG 1 cut(s) 745
SmiMI CAYNNNNRTG 2 cut(s) 551, 584
SmlI CTYRAG 1 cut(s) 745
SmoI CTYRAG 1 cut(s) 745
SphI GCATGC 1 cut(s) 169
Sse9I AATT 7 cut(s) 172, 232, 336, 757, 837, 862, 889
SspDI GGCGCC 1 cut(s) 146
SspMI CTAG 2 cut(s) 765, 875
SstI GAGCTC 1 cut(s) 453
TaaI ACNGT 6 cut(s) 358, 410, 538, 586, 735, 809
TaiI ACGT 1 cut(s) 704
TaqI TCGA 4 cut(s) 132, 222, 746, 755
TaqII GACCGA 2 cut(s) 332, 737
TasI AATT 7 cut(s) 172, 232, 336, 757, 837, 862, 889
TatI WGTACW 1 cut(s) 46
TauI GCSGC 5 cut(s) 90, 102, 147, 165, 511
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 2 cut(s) 540, 892
Tru9I TTAA 2 cut(s) 540, 892
TscAI CASTG 2 cut(s) 738, 790
TseFI GTSAC 3 cut(s) 22, 586, 787
TseI GCWGC 3 cut(s) 141, 516, 819
Tsp45I GTSAC 3 cut(s) 22, 586, 787
TspDTI ATGAA 3 cut(s) 28, 535, 569
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 2 cut(s) 738, 790
Tth111I GACNNNGTC 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 617
XceI RCATGY 1 cut(s) 169
XhoI CTCGAG 1 cut(s) 745
XspI CTAG 2 cut(s) 765, 875
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.