RLG00000007284

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013145
Physical Location & Seq
Reverse (-)
15311121 .. 15312199
1079 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007284

Sequence Viewer

Length: 882 bp
ATGAGGTCTCTCTCAAAACCAGTCACCATCATCAACTTCATAGTAGTACTCCTCTTTTTCCCTACCCTAACTCTCTCCCAGTGCCGCGACTCCTGCGGCGACATCGCCATAAAGTACCCTTTTGCCCTCGATGATGGCTGCGGCGCTCCGCAGTTCCGCCGCATGCTCAACTGCTCCACCGACCTCTTCTTCCTCACCCCGTCCGGCTCCTACAAAGTCGAGTCCATCAACTACGACAAGAAAACCATCGTCGTTTACGACCCCGCCATGTCCACCTGCTCCATCCTCCAGCCCCACCACGACTTCCTCATGACCGACATCCAGTCAGCCCTAATCCCCCCTACCTCCGACACCGTCTTTGCCCTCCTCAACTGCTCAATTGACTCCCCTGTCCTCAACCACTACAGTTACCTCTGCTTCAACTTCTCTTCTCACTCCTGCGATGAGCTCTACGGCTCGTGCAACGCGTTTAGGGTTTTCCACATGGTTGCCAACACTACTTCGCCGCCGTGCTGCTTTACAGGTTACGACACCGTTAAGTTCATGAGCATGAACGTCTTGGACTGCACGCATTACACCACGGTGACGAACACGGATGAGTTGAGGGGGATTGGACCGCAGGATTGGGTTTATGGGATCAAGATGACCTTCGCCGTGCCGGATTCCGGTTGCGAGAGGTGTGCGATGTCCGGTGGCACGTGCGGGTTTGATACAGAGACCGAAGGGGCACTGTGTCTTTGCTCGAGTTCGTCGAATTATACTAGAGAATGTGCTGTAGGCAGTGTCAGGGCGGGAGTGAACTGTGCAACCAGCAGCATACTCTTGCAATCAGTTTTTGTGATCGTGTTGATCTATAATTTGTTCAGCCTAGTACTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

294

Amino Acids

32.17

Weight (kDa)

4.98

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 87 8.3e-14 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 212 - 249 8e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17350 AT3G17350
fragaria_vesca FvH4_4g21130
malus_domestica MD16G1139400.v1.1
prunus_persica Prupe.1G108900_v2.0.a1
pyrus_communis pycom16g12380
rosa_chinensis RchiOBHm_Chr4g0426711
rosa_laevigata RLG00000007284
rosa_multiflora Rmu_co8392541.1_g000001 Rmu_sc0010934.1_g000002 Rmu_sc0021908.1_g000002
rosa_roxburghii Rroxscaffold_5G00368680
rosa_rugosa Rorug04G0214400
rosa_samantha Rh4AG269400 Rh4BG275700 Rh4CG290200 Rh4DG272700
rosa_wichuraiana Rw4G023370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
AasI GACNNNNNNGTC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 284
AccII CGCG 2 cut(s) 87, 467
AclWI GGATC 1 cut(s) 644
AcvI CACGTG 1 cut(s) 699
AfaI GTAC 3 cut(s) 48, 116, 873
AfiI CCNNNNNNNGG 1 cut(s) 665
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 1 cut(s) 421
AhdI GACNNNNNGTC 1 cut(s) 322
AleI CACNNNNGTG 1 cut(s) 581
AluBI AGCT 1 cut(s) 448
AluI AGCT 1 cut(s) 448
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 12, 710
AlwI GGATC 1 cut(s) 644
Ama87I CYCGRG 1 cut(s) 742
ApeKI GCWGC 3 cut(s) 138, 513, 813
ArsI GACNNNNNNTTYG 2 cut(s) 341, 373
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 614
AsuHPI GGTGA 3 cut(s) 16, 187, 595
AvaI CYCGRG 1 cut(s) 742
AvaII GGWCC 1 cut(s) 614
BaeGI GKGCMC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 450
BauI CACGAG 1 cut(s) 457
BbrPI CACGTG 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 125, 500, 825
BccI CCATC 5 cut(s) 35, 128, 233, 254, 290
BceAI ACGGC 3 cut(s) 469, 493, 638
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BcoDI GTCTC 2 cut(s) 12, 710
BfaI CTAG 2 cut(s) 762, 869
BfmI CTRYAG 2 cut(s) 403, 774
BfoI RGCGCY 1 cut(s) 147
BfuAI ACCTGC 1 cut(s) 284
BisI GCNGC 8 cut(s) 85, 97, 139, 142, 160, 506, 514, 814
BlsI GCNGC 8 cut(s) 86, 98, 140, 143, 161, 507, 515, 815
BmcAI AGTACT 2 cut(s) 48, 873
Bme18I GGWCC 1 cut(s) 614
BmeRI GACNNNNNGTC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 742
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 1 cut(s) 208
BmrI ACTGGG 1 cut(s) 73
BmuI ACTGGG 1 cut(s) 73
BpmI CTGGAG 1 cut(s) 272
BsaAI YACGTR 1 cut(s) 699
BsaI GGTCTC 2 cut(s) 12, 710
BsaJI CCNNGG 1 cut(s) 579
BsaWI WCCGGW 2 cut(s) 665, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 665
Bse1I ACTGG 3 cut(s) 20, 79, 322
BseDI CCNNGG 1 cut(s) 579
BseGI GGATG 3 cut(s) 282, 318, 601
BseLI CCNNNNNNNGG 1 cut(s) 665
BseNI ACTGG 3 cut(s) 20, 79, 322
BseRI GAGGAG 2 cut(s) 41, 356
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 3 cut(s) 125, 500, 825
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 2 cut(s) 87, 467
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 1 cut(s) 742
BsiSI CCGG 4 cut(s) 204, 659, 666, 690
BslI CCNNNNNNNGG 1 cut(s) 665
BsmAI GTCTC 2 cut(s) 12, 710
Bso31I GGTCTC 2 cut(s) 12, 710
BsoBI CYCGRG 1 cut(s) 742
Bsp1286I GDGCHC 2 cut(s) 450, 730
Bsp143I GATC 3 cut(s) 636, 840, 849
BspFNI CGCG 2 cut(s) 87, 467
BspHI TCATGA 2 cut(s) 309, 543
BspLI GGNNCC 1 cut(s) 208
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 1 cut(s) 644
BspTNI GGTCTC 2 cut(s) 12, 710
BsrI ACTGG 3 cut(s) 20, 79, 322
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 3 cut(s) 636, 840, 849
BssSI CACGAG 1 cut(s) 457
Bst2BI CACGAG 1 cut(s) 457
Bst4CI ACNGT 6 cut(s) 355, 407, 535, 583, 732, 803
Bst6I CTCTTC 2 cut(s) 191, 433
BstBAI YACGTR 1 cut(s) 699
BstC8I GCNNGC 2 cut(s) 164, 569
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 3 cut(s) 282, 318, 601
BstFNI CGCG 2 cut(s) 87, 467
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 3 cut(s) 639, 843, 852
BstMAI GTCTC 2 cut(s) 12, 710
BstMBI GATC 3 cut(s) 636, 840, 849
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNSI RCATGY 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 403, 774
BstSLI GKGCMC 1 cut(s) 730
BstUI CGCG 2 cut(s) 87, 467
BstV1I GCAGC 3 cut(s) 125, 500, 825
BtgI CCRYGG 1 cut(s) 579
BtgZI GCGATG 3 cut(s) 88, 456, 698
BtsCI GGATG 3 cut(s) 282, 318, 601
BtsI GCAGTG 1 cut(s) 787
BtsIMutI CAGTG 3 cut(s) 86, 728, 787
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 2 cut(s) 164, 569
CciI TCATGA 2 cut(s) 309, 543
CfoI GCGC 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 614
Csp6I GTAC 3 cut(s) 47, 115, 872
CviAII CATG 6 cut(s) 163, 268, 310, 484, 544, 550
CviJI RGCY 7 cut(s) 138, 207, 292, 329, 448, 456, 867
CviKI_1 RGCY 7 cut(s) 138, 207, 292, 329, 448, 456, 867
CviQI GTAC 3 cut(s) 47, 115, 872
DpnI GATC 3 cut(s) 638, 842, 851
DpnII GATC 3 cut(s) 636, 840, 849
DrdI GACNNNNNNGTC 1 cut(s) 389
DriI GACNNNNNGTC 1 cut(s) 322
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 2 cut(s) 191, 433
Eam1105I GACNNNNNGTC 1 cut(s) 322
EarI CTCTTC 2 cut(s) 191, 433
EciI GGCGGA 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 450
Eco31I GGTCTC 2 cut(s) 12, 710
Eco47I GGWCC 1 cut(s) 614
Eco53kI GAGCTC 1 cut(s) 448
Eco72I CACGTG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 450
FaeI CATG 6 cut(s) 166, 271, 313, 487, 547, 553
FalI AAGNNNNNCTT 2 cut(s) 632, 664
FatI CATG 6 cut(s) 162, 267, 309, 483, 543, 549
FauI CCCGC 3 cut(s) 271, 695, 784
Fnu4HI GCNGC 8 cut(s) 85, 97, 139, 142, 160, 506, 514, 814
FokI GGATG 3 cut(s) 269, 305, 608
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 8 cut(s) 85, 97, 139, 142, 160, 506, 514, 814
FspBI CTAG 2 cut(s) 762, 869
GlaI GCGC 1 cut(s) 145
GluI GCNGC 8 cut(s) 85, 97, 139, 142, 160, 506, 514, 814
GsuI CTGGAG 1 cut(s) 272
HaeII RGCGCY 1 cut(s) 147
HapII CCGG 4 cut(s) 204, 659, 666, 690
HhaI GCGC 1 cut(s) 146
Hin1II CATG 6 cut(s) 166, 271, 313, 487, 547, 553
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HinfI GANTC 4 cut(s) 89, 221, 383, 662
HpaII CCGG 4 cut(s) 204, 659, 666, 690
HphI GGTGA 3 cut(s) 16, 187, 595
Hpy166II GTNNAC 3 cut(s) 256, 273, 799
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 4 cut(s) 310, 544, 640, 879
Hpy8I GTNNAC 3 cut(s) 256, 273, 799
Hpy99I CGWCG 2 cut(s) 254, 754
HpyAV CCTTC 2 cut(s) 658, 716
HpyCH4III ACNGT 6 cut(s) 355, 407, 535, 583, 732, 803
HpyCH4IV ACGT 2 cut(s) 555, 698
HpyCH4V TGCA 4 cut(s) 462, 567, 806, 826
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpySE526I ACGT 2 cut(s) 555, 698
Hsp92II CATG 6 cut(s) 166, 271, 313, 487, 547, 553
HspAI GCGC 1 cut(s) 144
Kzo9I GATC 3 cut(s) 636, 840, 849
LmnI GCTCC 4 cut(s) 151, 179, 212, 284
Lsp1109I GCAGC 3 cut(s) 125, 500, 825
MaeI CTAG 2 cut(s) 762, 869
MaeII ACGT 2 cut(s) 555, 698
MaeIII GTNAC 4 cut(s) 22, 407, 524, 583
MalI GATC 3 cut(s) 638, 842, 851
MboI GATC 3 cut(s) 636, 840, 849
MboII GAAGA 3 cut(s) 178, 181, 420
MfeI CAATTG 1 cut(s) 378
MhlI GDGCHC 2 cut(s) 450, 730
MluCI AATT 3 cut(s) 378, 754, 856
MluI ACGCGT 1 cut(s) 465
MlyI GAGTC 3 cut(s) 83, 230, 377
MmeI TCCRAC 1 cut(s) 372
MseI TTAA 1 cut(s) 537
MslI CAYNNNNRTG 2 cut(s) 548, 581
MspI CCGG 4 cut(s) 204, 659, 666, 690
MunI CAATTG 1 cut(s) 378
MvnI CGCG 2 cut(s) 87, 467
MwoI GCNNNNNNNGC 1 cut(s) 93
NdeII GATC 3 cut(s) 636, 840, 849
NlaIII CATG 6 cut(s) 166, 271, 313, 487, 547, 553
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 22, 583
NspI RCATGY 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 581
PaeI GCATGC 1 cut(s) 166
PaeR7I CTCGAG 1 cut(s) 742
PagI TCATGA 2 cut(s) 309, 543
PaqCI CACCTGC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 3 cut(s) 255, 464, 749
PfeI GAWTC 1 cut(s) 662
PflFI GACNNNGTC 1 cut(s) 353
PkrI GCNGC 8 cut(s) 86, 98, 140, 143, 161, 507, 515, 815
PleI GAGTC 3 cut(s) 83, 229, 377
PmaCI CACGTG 1 cut(s) 699
PmlI CACGTG 1 cut(s) 699
PpsI GAGTC 3 cut(s) 83, 229, 377
Ppu21I YACGTR 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 450
PspCI CACGTG 1 cut(s) 699
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 1 cut(s) 614
PspXI VCTCGAGB 1 cut(s) 742
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 3 cut(s) 48, 116, 873
RsaNI GTAC 3 cut(s) 47, 115, 872
RseI CAYNNNNRTG 2 cut(s) 548, 581
SacI GAGCTC 1 cut(s) 450
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 8 cut(s) 85, 97, 139, 142, 160, 506, 514, 814
Sau3AI GATC 3 cut(s) 636, 840, 849
Sau96I GGNCC 1 cut(s) 614
ScaI AGTACT 2 cut(s) 48, 873
SchI GAGTC 3 cut(s) 83, 230, 377
SduI GDGCHC 2 cut(s) 450, 730
SfcI CTRYAG 2 cut(s) 403, 774
Sfr274I CTCGAG 1 cut(s) 742
SinI GGWCC 1 cut(s) 614
SlaI CTCGAG 1 cut(s) 742
SmiMI CAYNNNNRTG 2 cut(s) 548, 581
SmlI CTYRAG 1 cut(s) 742
SmoI CTYRAG 1 cut(s) 742
SphI GCATGC 1 cut(s) 166
Sse9I AATT 3 cut(s) 378, 754, 856
SspMI CTAG 2 cut(s) 762, 869
SstI GAGCTC 1 cut(s) 450
TaaI ACNGT 6 cut(s) 355, 407, 535, 583, 732, 803
TaiI ACGT 2 cut(s) 558, 701
TaqI TCGA 4 cut(s) 129, 219, 743, 752
TaqII GACCGA 2 cut(s) 329, 734
TasI AATT 3 cut(s) 378, 754, 856
TatI WGTACW 2 cut(s) 46, 871
TauI GCSGC 5 cut(s) 87, 99, 144, 162, 508
TfiI GAWTC 1 cut(s) 662
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 3 cut(s) 86, 735, 787
TseFI GTSAC 2 cut(s) 22, 583
TseI GCWGC 3 cut(s) 138, 513, 813
Tsp45I GTSAC 2 cut(s) 22, 583
TspDTI ATGAA 3 cut(s) 28, 532, 566
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 3 cut(s) 86, 735, 787
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 614
XceI RCATGY 1 cut(s) 166
XhoI CTCGAG 1 cut(s) 742
XspI CTAG 2 cut(s) 762, 869
ZrmI AGTACT 2 cut(s) 48, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.